Document 7Oz12pmQbok5k3nVy2zMjBe0B

To: From: Cc: Bcc: Received Date: Subject: Priston, Robert A SC-CHSE-BP <Robert.A.Priston@OPC.shell.com>; Owen, David DE SC-CHSE-DO <David.E.Owen@OPC.sheILcom>; Gingell, Ralph R SCC <rgingell@SheILCom>; Veenstra, Gauke GE SC-CHSE-GV <Gauke. E. Veenstra@OPC.sheILcom> Cagen, Stuart Z SCC <lO=SHELLlOU=MSXSCC/CN=RECI PI ENTS/CN=SC724012> 2001-08-1020:39:44 GMT FW: animal testing spreadsheet 10-8-01.xls Bob: As per your Urgent, urgent, urgent request, Ralph and I have made significant additions to the spreadsheet. Below are some assumptions that were used to calculate some of the children's health animal numbers: Tier I. HPV battery - 130 rats Acute toxicity: LD50 5 animals/sex/dose, 5 doses = 50 rats. Could use less using up/down approach for oral, but not inhalation. SIDS Combined subchronic/reproduction. 10/dose/sex 4 doses, not counting offspring =80 rats Tier 2. 400 rats, 80 mice 2 generation: 250 rats [not including offspring as per your earlier note] one species developmental [25 females per dose, 4 doses] = 100 rats [Stuart - is a second species developmental required?] chromosomal aberration: none - in vitro mouse lymphoma: none - in vitro immunotoxicity: 10 mice per sex per dose, 4 doses = 80 mice metabolism: two routes, two doses, both sexes, 5 rats per group guesstimate 50 rats Tier 3. 750 rats, 400 mice. Adult neurotoxicity: 12 rats per sex/dose/4 doses, plus dose-setting plus recovery group: 150 rats developmental neurotoxicity: parental animals 12/sex/dose 4 doses; offspring ditto 200 rats, not counting unused offspring. two species carcinogenicity; 50 animals per sex per dose 4 doses; 400 rats, 400 mice I have also added acetone and MEK as chilren's health entries under the solvents product area. made some educated guesses about animal numbers regarding some of the butadiene/ethylene/propylene approved programs. Gauke: I think the BPA numbers are fairly accurate but some of the animal numbers are my best guess. Please check for accuracy as per your recollection. Finally, for completeness, I would ask about epidemiology studies. There is the extensive benzene Shanghai study, via a consortium, but there also other human studies, sole or consortia. I will be out of the office until Thursday of next week (16 August) but will be reachable via email, voice mail, or mobile. SH ELL-MCCLU RG-064243 I hope this helps. Don't be shy about asking Ralph and I for more information. Sewell Stuart Stuart Cagen, Ph.D., Staff Toxicology Advisor Shell Chemical Company, P.O. Box 4320, Houston, Texas 77210 tel: 713-241-1407; fax: 713-241-3325; email: szcagen@shellus.com mobile: 832-309-1984 -----Original Message----- From: Cagen, Stuart Z SCC Sent: Friday, August 10, 2001 3:26 PM To: Cagen, Stuart Z SCC Subject: animal testing spreadsheet 10-8-01 .xls animal testing spreadsheet 10-... Attachments: animal testing spreadsheet 10-8-01.xls SH ELL-MCCLU RG-064244