Document 7OyOYBkk58X7N2ozmGp0Y9mmR

NOTE TO FILE - AR 226 DATE: March 28, 2001 FROM: Karen Lannon SUBJECT: 3/26/01 Perfluoroalkanoic acids VC0^0 /o o ? Rich Purdy, an independent toxicologist, gave the attached presentation to EPA on March 26, 2001. The meeting attendee list is also attached. RECEIVED cCtToD T"OO o f i\) :rr. o oO cn CO Contain NO Cl I Perfluoro fatty acids F(CF2)xCOO F rom : Perfluorooctanoic acid (PPOA) Perfluoro telo 11 er alcoh ols F(CF2)xCH2CH2OH Perfluoro teloi i a er su lfo n a te s Perfluoro teloi a a er iodides -om^n! 2 Perfluorooctanoic acid (PFOA) Not a Problem like PFOS: Not as bioaccum ulative Not as toxic Not Found in w ildlife tissu es or human blood r0nisii 3 Or is it? Relative Toxicity oln a PFOS rat reproduction study the LOBL for the dams was 48 ppm liver (0569) oln a rat PFOA feeding study the female LOBL was 0.3 ppm liver (0445) Wildlife PFOA Levels O 0.04 ppm in California sea lion liver (0962) O 0.18 ppm in albatross liv e r (0962) Detection lim its high Little homologue monitoring 'Oni^fnh H 28 day rat gavage dosing followed by 28 days depuration (0951) 3mg/kg/day PFOS 30mg/kg/ day telomer alcohol Depuration phase data Chemical Dav 1 Dav 14 Dav 28 PFOS 377 240 191 Telomer alcohol PFOA 0.164 0.016 0 C10 1.7 1.4 2.2 C12 0.12 0.08 0.1 Ocnifein s Perfluoro fatty acids look like PFOS: Electronegative rods w ith a negative charge at one end Are th ey in th e sam e class? Sim ilarities: 1. Extrem ely P ersistent 2. Surfactants-less than m onolayer. 3. Bioaccum ulation M echanism: Enterohepatic recycling (0008 &0449) 4. M echanism o f T oxicity a. M itochondrial U ncoupling (0167 &oi69) b. W asting Syndrom e i. PFOS 1. 53 wk feeding (0956) 2. 7 day feeding (Haughom and Spydevold) w'"' v 'W` 7 3. 90 day feeding (0139) 4. Rabbit 13 day gavage (0949) 5. Fish ? 6. Quail 5 day feeding (0953) 7. 90 day rhesus (0137) 8 . 26 wk Cynomolgus (0957 and 0958) ii. PFOA 1. 13 wk feeding S t u d y (0449) 2. 7 day feeding (Haughom and Spydevold) 3. 50 m g/kg 14 days (Cook et al 1992) voir IVO s 4. carcinogenicity S t u d y (0437) c. Upregulate arom atase d. Lower C holesterol e. Not Carcinogenic  Other Possible Class Me 11 hers F(CF2)7 CFjjCHaCHaOH P = 1 .0 F(CF2)7 CF2CH2COOH P = 0.3 Alpha dihydro perfluoro fatty acids F(CF2)7 CF=CHCOOH + HF 0 .9 5 F(CF2)7 cf=choh + c o 2 P = 1 .0 F(CF2)7 c h f -c h o P = 1 .0 F(CF2)7 c h f -c o o h P = .05 Alpha monohydro perfluoro fatty acids. F(CF2)7 CHOH-COOH +HF P = 1 .0 F(CF2)7 CHO-COOH P=? F(CF2)7 c o o h I lo 3w 3 'J 03 MPD FLUORINE CHANNEL RESPONSE MINUTES (/Qi) Example of Incomplete M etabolism OR II I C8F 17s -n -c h 2c h 2o h \/ o C8F 17S-NH2 II 0 PFOSA 1 V PFOS u S p e c ie s PFO S ppb PFO SA L iv e r B ra n d t's c o rm o ra n t W h ite p e lic a n G re a t b lu e h e ro n R iv e r o tte r T u rtle P o la r B e a r M in k D o lp h in C h in o o k L a k e W h ite fis h B ro w n T ro u t 4 4 -2 1 0 0 1 5 -1 3 0 0 5 9 -1 1 0 0 3 3 -1 0 0 0 9 0 -7 0 0 1 7 3 -4 5 0 0 1 0 0 -4 9 0 0 1 0 0 -4 0 0 3 3 -1 7 3 3 3 -7 8 2 L -2 6 1 3 -9 4 <Q L < Q L -1 9 < Q L -7 1 <Q L < Q L (1 0 ) < Q L -5 5 0 1 2 0 -3 5 0 < Q L (1 9 ) < Q L (1 9 ) < Q L (1 9 ) M u s c le C arp 4 3 -3 0 0 < Q L (1 3 ) C h in o o k < Q L -1 9 0 < Q L (1 3 ) L a k e W h ite fis h 9 7 -1 7 0 < Q L (1 3 ) N D = n o n d etect d =d etected Q L=Q u an titatio n lim it PFHS d d d d d nd d d nd nd nd nd nd nd PFO A <Q L <Q L < Q L (7 2 ) < Q L (7 2 ) <Q L < Q L (7 2 ) < Q L (7 2 ) < Q L (7 2 ) < Q L (3 6 ) < Q L (3 6 ) < Q L (3 6 ) ontainNOCB1 Propose PFOA same Class as PFOS 1. Look alike 2. Surfactants 3. Very persistent 4. Biomagnify via enterohepatic recirculation 5. Uncouple mitochondria 6. Cause wasting 7. Up-regulate aromatase 8. Reduce serum cholesterol 9. Not carcinogens r'O i: IM r <s/j(,/o/ Ohv 4jfc*-K<fic S ~/ld>h=.----------- jA ^ T x ) r-- /Wlgf"lAfethi'iQTwJ hf<A ~jx= Uoer'^kc-AJt-z\&l\lS&t7u>.-A_ A> cao dC4> m > /-^ /rtn ? C5 /^ X fu / j , <?J abo 7^^ ox ll ^?(O"-S5/A (tc iX ) ^ (_2ci 2^0-K^ 2'O CLcZT^ j ac> 1!mi v-'j-, 'Z'bi'Z C- 3 3 ^ 2 < T 'l/ '-! ? y ? CV~_ (_ ^ O C ^ C -C ^X X Z - X-kO- ^ <Lr!3 ^ 1 ^ / <3 3>Y> t A cS^3uv^. W txsd P*wb>-es/tky "^WV>u Gnrl VVax^ ccJ O g u a \ a \ cS l-y bUi^ ^ t^ f\fJ ^ (ce A^L^ C o j> E&T^p/ ic C4>l oxn> <=>-c>- 153 S ^Ib ' / 7 3 b Tba-bo-^S^I 7^32-r3.-2- o "3 S<f2-^ -.oOs^fr Z2. Z60. tZ?! %cU 13.lJ)tr fu s M n 7/&*/2-Y Contain NO CFM /r