Document 7Oq3NB7Rr3MZqjXE96V3Km3ro

M A&6 - 0 ALGICIDAL ACTIVITY TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-203. Current information indicates it is a mixture of 1.34% PFS, 35% diethylene glycol butyl ether, 37.85% water, 20% ethylene glycol, 2.66 % Sultone foamer, 3% sodium octyl sulfate, 0.1% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Standard No. 22, Relating to Swimming Pool Water Treatment Chemicals and/or Processes (National Sanitation Foundation) Type (test type): Acute Static GLP: No Year study performed: 1974 Species: Chlorella pyrenoidosa and Phormidium inundatum Source: In-house cultures maintained at Industrial Bio-test Laboratories, Inc., Northbrook, IL. Element basis: Cell populations calculated from hemocytometer counts, turbidimetric measurement. Exposure period: 14 days Statistical Methods: Not given. Analytical monitoring: Not given. Test Conditions: Algal Nutrient Medium: Allen's nutrient medium. Stock and test solution preparation: Algal solutions prepared by blending algae with 100 mL of Allen's medium using a Waring Blender. One mL aliquots of the test substance were handled as 100% active and diluted with distilled water and added to Allen's medium. One mL of adjusted cell suspension was added to each test solution, for a final volume of 100 mL. Exposure vessels: Flasks containing final volume of 100 mL. Number of replicates: 1 Initial cell loading: 7.5 x 104 cells/mL for Chlorella, 12.5 x 104 cells/mL for Phormidium 000574 Number of concentrations: six plus a blank control for Chlorella, five plus a blank control for Phormidium. Lighting: Not given Subculture: Inoculated bottles subcultured to fresh medium after one day incubation, with final readings made after 14 days inoculation. Three dilutions of the test substance were subcultured (1:5,1:10, and 1:100). Water Chemistry: Not given. RESULTS Nominal concentrations: Bk control, 1:5,1:10, 1:100,1:150,1:1000, and 1:10000 dilution for Chlorella pyrenoidosa study. Bk control, 1:5, 1:10,1:100, 1:1000, and 1:10000 dilution for Phorinidium inundatum study. Results of the Study with Chlorella pyrenoidosa Sample (dilution) Control 1:5 1:10 1:100 1:150 1:1000 1:10000 1:5 subculture 1:10 subculture 1:100 subculture Gross Appearance (Color) green none none none Slight green tint green green none none none Turbidity (absorbance) >2.00 <0.01 <0.01 <0.01 0.03 >2.00 >2.00 <0.01 <0.01 <0.01 Results of the Study with Phorinidium inundatum Sample (dilution) Control 1:5 1:10 1:100 1:1000 1:10000 1:5 subculture 1:10 subculture 1:100 subculture Gross Appearance (Color) green none none none green tint green tint none none none Turbidity (absorbance) >2.00 <0.01 <0.01 <0.01 >2.00 >2.00 <0.01 <0.01 <0.01 Element values are based on dilutions of nominal concentrations. 000575 Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ Algicidal rather than algistatic action of the test substance was obtained. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_______________________________________________ Reliability: Klimisch ranking = 3. Study lacks description and records of the method followed. The sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_________________________________________________ Test was conducted by Industrial Bio-Test Laboratories, Inc., of Northbrook, IL at the request of the 3M Company, St. Paul, MN, 1974. OTHER______________________________________________________ Last changed: 6/27/00 000576 MICROBIOLOGY DEPT. B I O -TEST Jtcrf}oicjQ>ue,f Dm. \I8 I0 FRONTAGE ROAD NORTHBROOK, ILLINOIS 60062 FDA RES. NO. I -t4 B 1 8 R EPO R T TO 3M COMPANY ALGICIDAL ACTIVITY OF TWO SAMPLES LABELED "A" AND "B " 7c* 2o*J JANUARY 23, 1974 IBT NO. 633-04473 000577 jjt h tih ia l B I O - T E S T jt ahofidto>Uei, 9*te. REPORT TO 3M COMPANY ALGICIDAL ACTIVITY OF TWO SAMPLES LABELED "A" AND "B" JANUARY 23, 1974 IB T N O . 633-04473 I. Introduction A t the req u est of 3M Com pany, te s ts w ere conducted to d eterm ine the algicidal activity of two sam ples identified as "A" and "B ". Two species of alg ae, C hlorella pyrenoidosa and P horm idium inundatum , w ere used in the eveluation. ) 000578 fneUafokU B I O - T E S T la k a fo U ei, 9ttc. 2 ~*) II. S u m m a ry Two sam ples, identified as "A" and "B ", w ere subm itted on N o v em b er 20, 1973, by 3M Com pany. The a lg ic id a l t e s t w as in itia te d on N ovem ber 30, 1973, using two sp e cie s of alg ae, C h lo rella pyrenoidosa W ise. 2005, P horm idium inundatum W ise. 1093. The te st was conducted according to a m ethod outlined and p rep ared by the N ational Sanitation Foundation, Standard No. 22, R elating to Swimming Pool W ater T reatm en t C hem icals a n d /o r P ro c e sse s. The turbidim etric determ ination conform ed to the pro cedure recom m ended by F itzg erald .* A 1:100 dilution of Sam ple "A " or "B ", effectiv ely co n tro lled the p ro liferatio n of C hlorella sp. and P horm idium sp. fo r a 14-day period. C idal ra th e r than static action of the candidate lgacides w as obtained. * F itzgerald, G. P. Bioassay for A lgicidal v ersu s A lgistatic C hem icals, W ater and Sewage W orks, 110-296 (1963) R espectfully subm itted, INDUSTRIAL BIO-TEST LABORATORIES, INC. R e p o rt p re p a re d by: D aniel Chan, M. S. G ~' R e p o rt approved by: Ja n u a ry 23, 1974 dp A. David B askin, P h. D. Section Head, M icrobiology J 000579 SmduUnial B I O - T E S T Jtcrfunaii>>dei;f*& 3 HI. P ro ced u re A. P re p ara tio n of Inoculum Two species of algae, C hlorella pyrenoidosa W ise. 2005, and P horm idium inundatum W ise. 1093, w ere used as the te st organism s. Each organism was subcultured at weekly in terv als in A llen 's neutrient m edium for three w eeks, p rio r to inception of the te st. The suspensions of C hlorella and Phorm idium w ere adjusted to co n ta in 7. 5 x 10^ and 12. 5 x 10^ c e l ls /m l r e s p e c tiv e ly . T h ey w e re p repared by shaking the algae growth in the fla sk s, to which w as added 100 m l of A lle n 's m edium and b le n d e riz e d fo r one m inute in a W aring B lender. Cell populations w ere calculated fro m hem ocytom eter counts. B. P re p ara tio n of Sample One m l a liq u o ts of t e s t sa m p le s h a n d le d a s 100% a c tiv e w e r e diluted in distilled w ater and added to A llen's m edium for a final c o n c e n tra tio n ranging fro m 1:5 to 1:10,000. T h ese c o n c e n tra tio n s w e re achieved a fte r the addition of 1 m l of the adjusted algae cell su sp en sio n fo r a fin al volum e o f '100 m l. The n u m b ers of C h lo re lla and P h o rm id iu m c e lls used w e re 7. 5 x 10 and 12. 5 x 10* re s p e c tiv e ly . Six control flask s, two per species, w ere also prepared. A ll inoculated bottles w ere subcultured to fre sh m edium after one day incubation. F inal readings for the presence of algal growth in all bottles w ere m ade fourteen days after inoculation. Jkidtiiinlal B I O - T E S T JaiotafoiUA, $*to. 4 I IV. R esults The re su lts of the grow th exam ination, and tu rb id im etric m ea su re m ent of all flask s inoculated w ith C hlorella pyrenoidosa and Phorm idium inundatum used for the algicidal te sts a re presented in Table I and II respectively. ) \ J 0005S1 jndudiU cfl B I O - T E S T JaiotavU&t, ne. 5 ) TABLE I R esults of the G ross and T urbidim etric E xam ination of C hlorella pyrenoidosa A lgicidal T est Sam ple "A " (dilution) Gross A ppearance (color) T u rb id ity (absorbance) 1:5 1:10 1:100 1:150 1:1, 000 1:10.000 1:5 su b c u ltu re 1:10 su b cu ltu re 1:100 su b cu ltu re C o n tro l none none none slight green tint green green none ------- - none none green <0.01 <0.01 <0.01 0.03 > 2.00 >2.00 ' <0.01 <0.01 < 0.01 > 2.00 Sam ple "B" 1:5 1:10 1:100 1:150 1:1,000 1:10,000 1:5 su b c u ltu re 1:10 su b cu ltu re 1:100 su b cu ltu re C o n tro l none none none slight green tint green green none none none green < 0.01 <0.01 <0.01 0.03 >2.00 >2.00 < 0.01 < 0.01 < 0.01 >2.00 ; / >0 7 ' ` ^ / *. ' ! 000588- Onduinidl B I O - T E S T jtato*ato*ie&, Omo. 6 TABLE II R e s u lts of the-. G ro s s and T u r b id im e tr ic Exam ination of Fhorinidium inundatum A lgicidal T est S a m p le MA" (dilution) G ross Appearance (color) T u rb id ity (A bsorbance) 1:5 none *1:10 none l i 100 none 1:1,000 green tint -LOOxO__________ --------- .g re e n tin t 1:5 subculture none 1:10 subculture none 1:100 subculture none C o n tro l green < 0.01 < 0.01 . <0.01 >2.00 >2.00 <0.01 <0.01 < 0.01 > 2. 00 Sam ple "BM 1:5 1:10 1:100 1:1,000 1:10,000 1:5 sub cu ltu re 1:10 subculture 1:100 subculture C o n tro l none none none green tint green tint none none none green < 0.01 < 0.01 < 0.01 >2.00 > 2.00 . < 0.01 < 0.01 . < 0.01 > 2.00 0005S3