Document 7OLw6M467ojZReDYwX6XRJQJE

- i o 1 1 1 1 1.141 1 1 t i TECHNICAL R EPORT !SUMMARY BACK TO MAIN TO: TECHNICAL COMMUNICATIONS CENTER - POI-XN (Importaiit I f report i s printed on both sides of paper, Eend two copies to TCC.) Division E n v i r o n m e n t a l Laboratory (EE & PC), Pr<,lrw I --. t i c T o u.c i. t v TO E. A. Reiner 8 Au thor(rt Testing: C S-2151. Ni>lul,ook Rmfuronco St. LOt Patul, Minnesota 3 (LR 626OS) 0-t. Nu?- 0535 Prolmct Numbu Ro9po9rt7N0um0b1mr2600 I 046 Employr Numkrld 46981 No. of P m s lncludino Coverahhnt SECURlTV I2 Open (Company Confidential) 0 Clod (special hthorization) 3M CHEMICAL REGISTRY New Chomicelr Reported 0 Ya 9 No K EVWORDS: (Select terms from 3M Thesaurus. Suggest other applicable terms.) EE & PC - D i v . (Env. Lab) URRENT OBJECTIVE: To d e t e r m i n e t h e a c u t e a q u a t i c LC f o l l o w i n g exposure t o v a r y i n g c o n c e n t r a t i o n s of cs-zfsl, L o t 3. Toxicity (Aquatic) tEPORT ABSTRACT: (200-250 words) This abstract informationis distributedby the Technical Communications center to llert 3M'rrr to Company R & 0 . It is Company confidential mterial. The a c u t e s t a t i c a q u a t i c 96-hour LC v a l u e s obtained f o r f a t h e a d minnows (Pime]- ~ro&las)following e x p o s u r e t o CS-2151 i n re:plicated e x p e r i m e n t s were 199 (172-231) a n d 153 (126-181) mIg/l. The combined 9 6 - h o u r LC50 w a s 175 (156-195) m g / l . D a t a g e n e r a t e d i n c l u d e LCs0 v a l u e s w i t h 95%c o n f i d e n c e limits. InitJs: BACK TO MAIN CONCLUSIOtJS In these replicated 96-hour LC5o a q u a t i c tests, t h e c a l c u l a t e d v a l u e s f o r f a t h e a d minnows ( P i m e p h a l e s promelas) were 199 and 1 5 3 mg/l. Materials p o s s e s s1 i n g a n LC50 of t h i s order of magnitude a r e c o n s i d e r e d t o be ] ? r a c t i c a . l l y n o n t o x i c based on the NIOSH c r i t e r i a for a q u a t i c t o x i c i t y i n t e r p r e t a t i o n . ( 2 ) INTRODUCTION: The test s u b s t a n c e is a l i g h t yellow l i q u i d , s o l u b l e i n water a t a m b i e n t room t e m p e r a t u r e . T e s t s perforined o n t h i s m a t e r i a l , evaluated its potential aquatic toxicit:y and the data generated form t h e b a s i s of t h i s report. ACCLIMATION AND EXPERIMENTAL - DESIGN: T h e t e s t protocol u t i l i z e d f o r t h i s s t u d y was modeled a f t e r t h a t described by ASTMrE729-80. (1) The f a t h e a d minnows ( P i m e p h a l e s promelas) were o b t a i n e d from a p r i v a t e h a t c h e r y ( a ) . Stock f i s h were Geld in a f l o w t h r o u g h system fiberglass holding tank :Eilled with carbon-filtered w e l l water a t 14-16 d e g r e e s C. A d a i l y photoperiod of 1 6 h o u r s l i g h t and 8 h o u r s d a r k , w i t h a 30-minute t r a n s i t i o n period was m a i n t a i n e d t h r o u g h o u t t h e a c c l i m a t i o n and t e s t i n g period. The f i s h were f e d T e t r a - M i n ( b ) d a i l y , food b e i n g w i t h h e l d 48 h o u r s p r i o r t o a n d t h r o u g h o u t t h e t e s t period. F i s h were acclimated t o t e s t t e m p e r a t u r e f o r 2 d a y s p r i o r t o usle i n a s s a y s . Twenty ( 2 0 ) f a t h e a d minnows, u n i f o r m i n s i z e , were tested a t each chemical. A l l - g l a s s a q u a r i a , 3 ' 5 x 2 0 ~ 2 5cm c o n t a i n i n g 1 6 l i t e r s of d i l u e n t p l u s t o x i c a n t comprised a s t u d y chamber. T e s t f i s h were randomly allocated t o each t e s t chamber w i t h i n 30 m i n u t e s f o l l o w i n g t o x i c a n t a d d i t i o n . The f a t h e a d minnow e x p e r i m e n t s were c o n d u c t e d a t 19+2 degrees C. M o r t a l i t y , t e m p e r a t u r e , d i s s o l v e d o x y g e n l e v e l , and pH of a l l t e s t s o l u t i o n s were measured a t 24-hour i n t e r v a l s or u n t i l t o t a l m o r t a l i t y had o c c u r r e d . General observations were r e c o r d e d r e l a t i v e t o swimming p a t t e r n and b e h a v i o r a l c h a n g e s induced by e x p o s u r e t o t h e t e s t chemical. The a c u t e short-term s t a i c b i o a s s a y s were performed o n CS-2151 u c sing onst car ant bonqua f l i i t lt y ere as d th w e el d l i l water uent of (Ta known bles 1 , co 2) m. p o s i t i o n and a ) Dale F a t t i g F i s h Farm, Brad:y, Nebraska. b ) Commercial F i s h Food of known c o m p o s i t i o n . BACK TO MAIN The 96-hour f i s h s t u d i e s were p e r f o r m e d i n r e p l i c a t e u s i n g t h e f o l l o w i n g CS-2151 l o g a r i t h m i c concentrations: 87, 135, 210 3 2 0 , . and 490 mg/l. The range o f d i l u t i o n s was s e l e c t e d t o b r a c k e t 9 6 - h r . LC50 v a l u e s p r e d i c t e d from p r e l i m i n a r y e x p l o r a t o r y toxicity tests. LC50 v a l u e s w i t h 95% c o n f i d e n c e l i m i t s were c a l c u l a t e d u s i n g t h e p r o b i t program and 3 M TRAC Computer s y s t e m . M o n i t o r i n g e q u i p m e n t u s e d i n c l u d e d ASTM t h e r m o m e t e r s , O r i o n temperature-compensating p H meter, a Y e l l o w S p r i n g s d i s s o l v e d oxygen meter, and a T a y l o r temperature chart r e c o r d e r . RESULTS : The f a t h e a d minnows ( P i m e hales promelas) used i n t h e s e s t u d i e s --fE---- had an a v e r a g e w e i g h t o 0.3 grains a n d a l e n g t h of 3.5 cm. The l o a d i n g f a c t o r was-0.4 g/l; t h e maximum r e c o h e n d e d l o a d i n g . f a c t o r is 0.8 g / 1 -( 1) T e m p e r a t u r e , pH, and d i s s o l v e d oxygen v a l u e s a n d r a n g e s o b t a i n e d d u r i n g t h i s s t u d y were: 19+ 20 C . , - 7 . 6 8.0 p H u n i t s , a n d 96-hour d i s s o l v e d o x y g e n value: r a n g e d - from 5.2 5.9 mg/l. T h e s e l a t t e r v a l u e s c a l c u l a t e d o u t t o a minimal oxygen saturation of 56-63%. I n this r e p l i c a t e d s t u d y , t h e f i s h 96-hour LC50 v a l u e s were 1 9 9 and 1 5 3 mg/l. The 1-, 2-, 3-, arid 4-day LC50 v a l u e s a n d t h e i r 95% confidence l i m i t s and t h e method used to calculate them are l i s t e d i n T a b l e 3. C h e m i c a l s p o s s e s s i n g a n LC50 v a l u e i n t h i s r a n g e a r e c o n s i d e r e d t o be p r a c t i c a l l y n o n t o x i c to t h e aquatic environment (2). The d a t a r e p o r t e d h e r e i n a r e b a s e d o n s t u d i e s p e r f o r m e d by M.T. Elnabarawy and R. R. Robideau. Accompanying d a t a s h e e t s comprise o r i g i n a l d a t a . A c o p y of t h i s r e p o r t is f i l e d i n t h e E n v i r o n m e n t a l L a b o r a t o r y A r c h i v e S y s t e m , B u i l d i n g 2-3E. I t should be noted t h a t t h e generated r e s u l t s w e r e reported in the a b s e n c e of i n f o r m a t i o n r e l a t i v e t o thre " p u r i t y " a n d t h e " s t a b i l i t y " of t h e c h e m i c a l s u b s t a n c e CS-,2151, L o t 3. The r e p o r t e d LC50 v a l u e s were c a l c u l a t e d o n t.he b a s i s o f t h e "observed m o r t a l i t y rates" and of t h e c a l c u l a t e d i n i t i a l concentrations o f CS-2151 i n test s o l u t i o n s . REFERENCES: (1) ASTM-Standard P r a c t i c e E729-80. S t a n d a r d P r a c t i c e f o r C o n d u c t i n g A c u t e T o x i c i t y T e s t s w i t h F i s h e s , Macroi n v e r t e b r a t e s , a n d a m p h i b i a n s . American S o c i e t y f o r T e s t i n g a n d Materials, P h i l a d e l p h i a , PA 1980. ( 2 ) Registry of Toxic E f f e c t s of Chemical Substances, '76 E d i t i o n , C h r i s t e n s e n , H. E., and E. J. F a i r c h i l d , Eds., p . XV and C i , U.S. Department of H e a l t h , E d u c a t i o n a n d W e l f a r e ; National I n s t i t u t e for Occupational Safety and H e a l t h , Washington, D.C. ( J u n e , 1 9 7 6 ) . BACK TO MAIN WELL WATER QUALITY SAMPLE DATE: 9/30/80 SAMPLE TAKEN: 3M Bldg. 2-3E, St.. Paul SAHPLE DATE 9/30/80 (TAKEN FROM TANK 14) PARAMETER CONCENTRATION ENVe LAB WATER QUALITY BOOK REF.. PH Residual Chlorine Ammonia Nitrogen Conductivity M-Alkalinity Total Solids Total Hardness Total Fluoride Dilute COD Total Coliform Standard Plate Count 7.7 8 (0.02 mg/l 9 (0.1 mg/l as N 8 380 umhos/cm at 25OC 9 230 mg/l a s CaC03 8 324 mg/l 0 244 mg/l a s CaC03 8 (0.02 mg/l 9 (0.2 mg/l 9 Negative 10 108 per m l 10 Aluminum as Al* 0.01 mg/l 14 Arsenic as As* <0.005 mg/l 14 Boron as B* <0.2 mg/l 14 Cobalt as Co* <0.002 mg/l 14 Copper as Cu* 0.018 mg/l 14 Chromium as Cr* (0.005 mg/l 14 Cadmium as Cd* (0.002 mg/l 14 Iron as Fe* 0.022 mg/l 14 Nickel as Ni* 0.005 mg/l 14 Lead as Pb* <0.005 mg/l 14 Silver as Ag* <O.O02 mg/l 14 Zinc as Zn* 0.13 mg/l 14 Mercury as Hg* <0.2 ug/l 14 PCB's** < 0 . 5 0 ug/l 36 Total Organochlorine** (0.50 ug/l 36 Pest i c ides Total <0.50 ug/l 36 Organophosphorus** Pesticides * Sample Date 3/31/80 **Sample Date 2/26/80 BACK TO MAIN TABLE 2 ~. .- ENVIRONMENTAL LABORATORY BLM;. 2-3E ST. PAUL, MINNESOTA THIRD QUARTERLY REPORT, 1 9 8 0 WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING PARAMETER TOTAL HARDNESS* as mg/l CaC03 TOTAL "M" ALKALINITY* as mg/l CaCO3 ELECTRICAL SPECIFIC CONDUCTANCE* a s umhos per cm2 a t 25% TOTAL SOLIDS* as mg/l pH** STANDARD PLATE COUNT*** per 1.0 m l JULY 31 256 AUG. 29 244 I SEPT. 30 AVERAGE (-+ S ) 252 2 5 1 -+ 6 221 219 223 2 2 1 -+ 2 380 390 360 377 -+ 5 368 404 350 374 -+ 27 7.6 7.6 7.6 7.6 -+ 0 119 94 Lab 1 0 7 -+ 18 Accident CRITERIA OF CONSTANT QUA.LITY * M o n t h l y r a n g e s s h o u l d be less t h a n 1 0 % of t h e i r respective a v e r a g e s . (ASTM S t a n d a r d E729-1980.) * * M o n t h l y r a n g e of pH s h o u l d b e l e s s t h a n 0 . 4 u n i t . E729-1980.) (ASTM S t a n d a r d *** T o t a l bacterial d e n s i t i e s s h o u l d n o t be g r e a t e r t h a n 500 organisms per m l . (USEPA-600/8-78-017, p. 101.)