Document 7O4R646YazR1V342eELED0qbR

4 K -2 2 6 _ Oi2Z BIODEGRADATION (BOD/COD) TEST SUBSTANCE_______________________________________ ____ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-3003. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not given. The test sample is FC-3003, identified by the laboratory as R1331. Current information indicates it is a mixture of 1% PFOS, 15% diethylene glycol butyl ether, 79.39% water, 2% Sultone foamer, 1.5% sodium octyl sulfate, 1% sodium decyl sulfate, 0.06% sodium lauryl sulfate, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect degradation of the fluorochemical component of the test sample. METHOD:__________________________________________________ _ Method: OECD Guideline. Type: Aerobic GLP: No Year completed: 1997 Contact time: 28 days Inoculum: Polyseed NX, Lot D257, Polybac Corporation, Bethelem, PA RESULTS____________________________________________________ Chemical Oxygen Demand (COD): 377,000 mg/kg Biochemical Oxygen Demand (CBOD): 5-Day: 132,000 mg/kg 10-Day: 247,000 mg/kg 20-Day: 296,000 mg/kg 28-Day: 368,000 mg/kg Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ No conclusions given. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 002687 DATA QUALITY Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. REFERENCES____________________________ _____________ This test was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number R1331, 1997. OTHER______________________________________________________ Last changed: 6/26/00 0026S8 AScI Corporation/AScI-Dulutb Environmental Testing Division Submitted to 3M Company - 8/97 AScI-DETD Study ID #5010-068 R1331 BOD REPORT FOR 3M COMPANY Submitted to 3M Environmental Technology and Safety Services 9 3 5 Bush Avenue Building 2-3E-09 PO Box 33331 St. Paul, MN 55133-3331 Prepared by AScI Corporation/AScl-Duluth Environmental Testing Division 4 4 4 4 Airpark Boulevard Duluth, Minnesota 55811 Sponsor: 3M Company Sponsor Study ID# R1331 AUGUST 1997 002689 COD = 377,000 mg Oz / kg chemical. Dates o f BOD test: 7/18/97 - 8/15/97. Water bath temperature range during the test: 19.8 - 2 0 .6 C . Lot number o f Polyseed NX: D257. AScI Corporation/AScl-Duluth Environmental Testing Division Submitted to 3M Company - 8/97 AScI-DETD Study ID #5010-068 TABLE 1. BOD Dilution Information for R1331 Sample ID (mg/L) 10 18 25 50 Stock Solution Concentration (m g/L) 1000.0 1000.0 1000.0 1000.0 Sample Volume (ml) 3 5.4 7.5 15 Sample Concentration (m g/L) L >: 10 18 25 50 TABLE 2. BOD Blank Calculations for R1331 Sample ID Blank Blank Blank Blank FIRST REPLICATE SECOND REPLICATE Day Initial D.O. Final D.O. D.O. Drop Initial D.O. Final D.O. D.O. Drp 5 8.90 8.84 0.06 8.91 8.90 0.01 10 8.90 8.45 0.45 8.91 8.42 0.49-- 20 8.90 8.30 0.60 8.91 8.42 0 .4 9 - 28 8.90 8.43 0.47 8.91 8.29 0 .6 2 ^ Sponsor: 3M Company Sponsor Study ID# R1331 - 1- 002(ET90 AScI Corporation/AScI-Dululh Environmental Testing Division Submitted to 3M Company - 8/97 AScI-DETD Study ID #5010-068 TABLE 3. BOO Seed Correction Factor Calculations for RT331 Seed Cone. Day I ml/Bottle Initial D.O. 10 5 ]1 8.85 ,0 10 11 8.85 ,0 20 8.85 ,0 28 8.85 FIRST REPLICATE Final D.O. D.O. Drop 5.40 3.45 1.89 6 .96 0.98 7.87 0.72 8.13 SCF* 0.69 1.39 1.57 1.63 Initial D.O. SECOND REPLICATE Final D.O. D.O. Drop 8.90 5.35 3.37 8.90 2.40 6.50 8.90 <0.5 -- 8.90 0.64 8.26 SCF 0.67 1.30 -- 1.65 a SC F = (D.O. drop * ml seed in rest) / ml seed in seed blank. Average SCF 0.68 1.35 1.57 1.64 {>- ^ TABLE 4 . BOD Reference8 and Tox Blanks Calculations for R1331 Cone. Day SCF mg/L 0 5 0.68 50 5 0.68 Initial D.O. 8.94 8.98 FIRST REPLICATE Final D.O. D.O. Corrected Drop Drop 4.04 4.90 4.22 <0.5 8.48 7.80 BOD mg/Lb 211 >390 Initial D.O. 8.90 8.95 SECOND REPLICATE Final D.O. Corrected D.O. Drop Drop 4.40 4.50 3.82 <0.5 8.45 7.77 i| BD mg/L J l9l I >389 1 r? * Reference = 150 mg glucose and 150 mg glutamic acid in 1 liter BOD dilution water, tested at 2% i dilution. b BOD mg/L = (corrected D.O. drop) * 300 ml BOD volume / 6 ml reference. Sponsor: 3M Company Sponsor Study ID# R1331 -2- 002691 TABLE 5. BOD Calculations for R1331 AScI Corporation/AScl-Duluth Environmental Testing Division Submitted to 3M Company - 8/97 AScI-DETD Study ID #5010-068 L1 Cone. Day SCF m g/L Initial D.O. 10 5 0.68 8.94 18 25 1 50 5 0.68 8.90 5 0.68 1! 8.91 5 0.68 J 8.95 10 10 1.35 8.94 IS 10 1.35 8.90 25 10 1.35 8.9) 50 10 1.35 8.95 10 20 1.57 8.94 15 20 1.57 8.90 25 20 1.57 8.91 50 20 1.57 8.95 FIRST REPLICATE j Final D.O. Corrected D.O. Drop Drop BOD Initial m g/kg* 1 D.O. 6.32 2.62 7.00 1.90 3.64 5.27 1.94 1.22 4.59 " 8.90 ~ 8.91 184,000 1 8.92 3.20 5.75 5.07 101,000 8.95 Mean 143,000 5.42 3.52 3.24 5.66 1.72 7.19 <0.5 8.45 2.17 217,000 4.31 239,000 5.84 234,000 7.10 > 142,000 Mean 230,000 8.90 8.91 8.92 8.95 4.50 4.44 2.12 6.78 0.72 8.19 <0.5 -- 2.87 287,000 5.21 289,000 6.62 265,000 -- Mean 280,000 8.90 8.91 8.92 8.95 SECOND REPLICATE Final D.O. Corrected D.O. Drop Drop 7.10 1.80 1.12 6.74 2.17 1.49 6.70 2.22 1.54 1.65 7.30 6.62 BOD ftig/kg __ .-- 132,000 4.96 3.94 3.27 5.64 1.50 7.42 <0.5 8.45 Mein 132,000 .y 1; 2.56 259,000 4.29 238,000 6.07 243,600 7.10 >142,000 Mean'247,000 i 3.75 5.15 3.58 358,000 2.54 6.37 4.80 267,000 0.74 8.18 6.61 264,000 <0.5 -- -- Megji 296,000 'h-r 10 28 1.64 8.94 4.00 4.94 . . 28 1.64 8.90 2.12 6.78 25 28 1.64 8.91 <0.5 50 28 1.64 8.95 <0.5 - 3.30 330,000 5.14 286,000 ---- --~ Mean 308,000 8.90 8.91 8.92 8.95 3.12 1.47 <0.5 <0.5 5.78 7.44 -- -- a BOD = corrected drop x 1,000,000 / test concentration. Omitted data points: D.O. drop corrected for SC F too low (< 2 .0 ). Final D.O. < 0 .5 mg/L. 4.14 414,000 5.80 322,000 -- ?: - - - -Mean 368,000 Sponsor: 3M Company Sponsor Study ID# R1331 3- - dn 002692