Document 7MxOLzdk7XdNdBg1RJ68QzRkj

BIO-MEDICAL RESEARCH DOCUMENT DESCRIPTION FORM 63 68 69 76 Duplicate in all cards: ~5> L oscjznro year as-1961- File number [Right justify [Numeric only] 77 78 Sub-Index Code Author(s), as Last Name FS (No Punctuation) and coden for journal as JAMA preceeded by one blank space 1 20 21 40 41 60 61 62 111 12 _____________________________________________________________ R&S 110564 Title of Report; end with space-hyphen-hyphen-space. Follow with Index Terms, separated from each other with comma-space. Avoid other punctuation; do not abbreviate. 61 62 21 22 23 24 Source (Journal, Vol., Number, Pages, Date ) 12 61 62 31 32 Brief Summary 12 10 SUMMARY: 61 62 61 62 63 64 C\ <n n &....... . ,j Q*> EXPERIMENTAL DATA ON THE BLASTOMOGENIC IN FECT OF CO'JMARON'H ANU POLYVINYL GILUkiDE PLATES K. I. Stankcvich [Kiev) The Ukrainian Scientific Research Institute of Communal Ily^ii-ne Translation of: "Hksperimental'nyye Dannyye 0 B1 automogennom in-istvii Kumarnnovykn i Polikhlorvinilovykh Plastin.ok," Fraciiebnoye Delo. 11, pp. 1 OB-114, llJ62. In recent years a number of experimental studies of Soviet (A. Kh. Kogan, L. M. Shabad) and foreign (Drukrey, Nortaurft, Oppenheimer, Turner ct al.) authors have been published indicating the development of tumors in animals upon intro ducing polymer plates into them. Among the tumors induced by polymer plates or metals, fibrosarcomas pre dominate (Yu. M. Vasil'yev). Data on the morphological changes of connective tissue which precede the appearance of sarcomas in experiments with polymer plates are extremely scattered (A. Kh. Kogan and I. T. Raykhlin, L. V. 01'shevskaya). In the party program adopted at the XXII Congress of the Communist Party of the Soviet Union, mention was made of the fact that the production of synthetic resins and plastics in the coming 20 years will be increased by approximately 60 times. Exceptionally wide application will also be found by plastics in living quarters; plastics will be used as floor covering materials. Among the most widespread plastics both in the USSR and abroad arc polyvinyl chloride and coumarone plates and polyvinyl chloride linoleum. Plastics have also been widely introduced into home economics and medicine. In connection with this, it becomes absolutely necessary to make a biological study of plastics with respect to their blastomogenic effect. This report is devoted to an experimental study of the blastomogenic properties of polyvinyl chloride and coumarone plates employed as floor coverings. The experiments were conducted on 310 albino rats weighing from 120 to 150 grams. All the animals were maintained on an ordinary diet in cages in a single room. The experimental animals were subdivided into four series. With regard to to the absence of experimental models, near the conditions of [TR note: word blurred] investigations on people, a method of parenteral implantation was employed. The plastics were implanted subcutaneously and [TR note: rest of sentence blurred] space. R&S 110565 1 \ 1 / The first series of experiments included animals in whom glass plates were implanted. The second series of experiments included animals with implanted .polyvinyl chloride plates, and the third series animals which were implanted with coumarone plates. The fourth series served as the control (intact animals). The first three series of experiments included 70 rats each and the fourth - 100 rats. The sizes of the implanted plates were as follows: subcutarieous -- 2.5 cm X 0.S cm X 0.2 mm; those near the kidney -- 2.0 cm X 1.2 cm X 0.2 mm. Prior to introduction into the animal's organism, the plates.were sterilized in 70 alcohol solution for a period of 5-7 days.- Administration of the implants to the rats was carried out under sterile conditions under ether anesthesia. The plates were implanted subcutaneously in the region of the right shoulder. Implantation in the abdominal cavity was carried out via [TR note: word blurred] section in the region of the right [word blurred]. After introducing the plates, the incision was closed with 2-3 catgut stitches. The surface of the incision was [TR note: 4 or 5 words blurred]. After the beginning (9, 10, 11 and 12 months) of the experiment the animals were killed by means of decapitation. From studying the effect of the implants, pathohistological investigations of the selected internal organs and tissues surrounding the plates-were carried out. At autopsy of the experimental rats the implanted -plates were removed together with the connective tissue capsules surrounding them. The connective tissue capsules as well as the internal organs (lungs, heart, spleen, liver, stomach, small and large intestines, kidneys) were fixed in 10% formalin. After this the capsules and sections cut from the organs [TR Note; several words not readable]. [TR Note: rest of paragraph not readable] The histological investigations (consultants V. S. Vitchenko and Professor M. K. Dalya) established that the capsules consist of fibrose connective tissue. In their histological structure all of the investigated capsules could be divided into two types. A significant majority of them -- 123 of 157 -- belonged to the first type. The capsule has a clearly defined fibrose structure. In it one can distinguish three zones: the inner zone, the mid zone and the outer zone. The inneir zone, directly adjacent to the plate, consists of fibrose connective tissue containing a large number of fibrocvtic cellular elements. The mid zone of the capsule also has a fibrose structure, but is more coarse than the inner structure and consists of tubrous bundles, hyalinized to a significant extent, composed throughout the length of the capsule of fibers and a small number of fibrocvtic cells. The outer zone has a finely fibrose structure; delicate collagen bundles are distributed in the pcricapsular lipid tissue over varying lengths. Vasculari zation in most capsules of the first type is well defined only in the outer zone. The content of cellular elements is occasionally extremely varied in individual regions. In most capsules one notes lymphoid cellular elements, histiocytes, and significantly loss frequently, leucocytes and clots of hemosiderin either diffusely scattered or in adjacent accumulations, occasionally taking the form of infiltrates. 2 \ 33 K(D 99SOVV f The remaining 34 capsules are of the second type with respect to histological structure. They also have a fibrose connective tissue structure. However in differentiation from capsules of the first type, the structure of these capsules . is not so orderly: fibrose bundles of different thickness are located in random fashion; in parts of the capsules and even along the course of individual fibrose bundles of the same capsule, there are different numbers of fibrocytic cellular elements. The lipid tissue through which these bundles pass is in places significantly sclerotic. Vascularization, in view of the porous structure of the capsule, is pronounced in most of these quite well. The inflamatory in filtration is similar to that in the capsules of the first type. The two structural variants of capsules which were established as existing about the plates are probably due to the individual degree of reactiveness of the organism to the experimental animals and the character of the inflamatory reaction to foreign bodies. ? No clear cut nistomorphological difference in overall structure between (/) capsules in experimental animals killed after 9, 10, 11 and 12 months was detected. - However during the microscopic investigation of preparations from the capsules of different series of experiments these characteristics were noted. CD4 In the series of experiments with implantation of glass plates (subcutaneous implantation) 42 capsules were investigated; of these 36 were investigated 11 months, and 6 after 1 year. Twenty capsules were of the first structural - type and 22 of the second. Mild proliferation of fibroblasts at the 11th month of the experiment occurred in 5 of the animals with localization in the inner and mid portions or only in the mid portions, while in the mid regions of two capsules proliferation of fibroblasts occurred a year after the beginning of the experiment. In the experiments in which glass plates were placed in the abdominal cavity near the kidney, 16 capsules were investigated; one after 10 months, 8 after 11 months (in one observation proliferation of fibroblasts was noted in the internal zone), and 7 after 1 year. Twelve capsules were of the first type and 4 were of the second type with respect to histological structure. In the series of. experiments with implantation, of polyvinyl chloride plates (the plates were introduced into the subcutaneous tissue), 32 capsules were'investi gated, of which 4 came from rats after 10 months, 14 after 11 months, and 14 after 12 months. Thirty capsules were of the first type of structure and 2 of the second type. In 20 capsules significant proliferation of fibroblastic cellular elements was noted, while in 6 of these there was cellular polymorphism. The proliferative processes in half the animals were localized in the internal zone, and in 5 capsules they encompassed the inner and mid zones (Figure 1), in S others they only included the mid region of the capsule. It was characteristic that in rats with implantation of the given type of plate, over the course of 10 months proliferation of fibroblasts was observed in the inner zone of 1 capsule; with an implantation time of 11 months proliferation was also noted only in the inner zone of 7 capsules. After 12 months the spread of fibroblasts was noted in the inner and mid regions of 4 capsules. In four rats the wall of the capsule was found to contain malignant tumorous growth in the form of sarcoma, in 2, polymorphiocc1lular (Figure 2) and in 2, ceils having a spindle shape (Figure 3). The first two appeared in the inner zone of the capsules 3 in animals in the 11th month of the experiment; 2 were localized in the mid zone as well as in the inner zone. In one experiment, at the 10th month of observation, a sarcoma weighing 99 grams bad developed surrounding the implanted plate; this sarcoma had a structure with very few polymorphic cellular elements [TR note: about 3 words not readable] in the 11th month from the beginning of the experiment [TR Note: about 3 or 4 more words missing] in another animal of the same observation series. Figure 1. Photomicrograph. Magnification 10 X 15. Pronounced proliferation of fibroblastic cellular elements encompassing the inner and mid zones. With the administration of polyvinyl chloride plates in the abdominal cavity lipid tissue, 32 capsules were investigated. They were all of the first type with respect to histological structure. The capsules were investigated after 10 months in one rat, after 11 months in 25 rats, and after 12 months in 6 rats. Proliferation of fibroblastic cellular elements without polymorphism was noted in 4 capsules after 11 months: in 2 it was noted in the inner zone and in 2 in the mid zones; after 12 months observation indicated this development in one capsule in all zones. All three zones of the capsule were invaded by sarcoma in one spindle cell capsule (after 11 months) and in one capsule consisting of polymorphous cells in regions characteristic of fibrosarcoma (after 11 months) [TR Note: part of sentence was not included in the copy and am sort of lost at this point]; there was one poly morphous capsule with regions characteristic of fibrosarcoma (after 12 months). In the series of experiments with implantation of coumarone plates (plates were introduced into the subcutaneous tissue), 21 capsules were investigated. Of these 16 were of the first type and 5 of the second type; 9 months after the beginning of the experiment capsules were removed from 14 rats, 10 months after the beginning of the experiment from 1 rat and 12 months after the beginning of the experiment from 6 rats. In three of the capsules removed after 9 months significant prolifer ation of fibroblastic cellular elements was noted twice in the mid zone, and in one capsule -- in the inner zone. In one capsule alter 12 months the inner and 'Md zones had developed a spindle cell sarcoma which ir. places had the form oi ! iL'res-'re sai. 4 l In tlit eiperl-implantation of coumarone plates in the abdominal tissue, 14 capsules -.urc investigated. ?/i.ch the exception of 1 capsule, all the capsules were of tuv f: rst type [Til .Note: a significant portion of text missed because of poor cop/]. In tv.-o capsules after 12 months the inner tone had a copious prolifer.': to- of f ib: oh 1 ast ic cellular elements; in one capsule after 12 months (with t!;` involvement of the mid cone as well), there was also copious proliferation of f:robl ustic cellular elements. [TR note: More text missing.] * I* * ** ' ** 4 * \* / ...u '.rifiVoiy/V/ \?r-s * vV* ,&'' /' */ '***' > *?. *V'* ^\, * / 'f' *ti* , i o 4 *N . * *i * *V v / <* v1 i vuy*\ *v- : 'ri -*,/ * ' " Figure 2. Photomicrograph. Magnification 20 X IS. Polymorphous Cellular Sarcoma. R&S 110569 Figure 3. Photomicrograph. Magnification 20 X IS, Spindle Cell Sarcoma. \ > The proliferation of fibroblastic cellular elements which occurred in 39 capsules of all experimental animals in which were noted in IS only in the inner regions were noted in 9 in the inner and mid regions simultaneously. Apnartently, [TR note: Text cannot be read -- text completely cut off on right mar;',in and some of the words are illegible from about the midpoint of civ pago to the right margin Most of the page (page 113) cannot be translated and I am discontinuing it here]. REFERENCES iv ;> c ii ,11, <> H IIM'I. At . f^l.- ,,-l Ki i'. m A, ,''tl*||,| (t A. I#lM, .V: I'i. ( p 11 *' x ;i n /*. ft. K ii r .i * A ,\\ IV: n *.. ij*jj.i t , :.......... ll.jiu.i iiMi.i n h n-j-.ii* . |*i , ; A.* ` |.||K(;, J'Vl .V' "t ` j\ I| I .11, \ V J* , I'.iin.iim II. T. Kill.. A. \ Ithi.f.i n im ii -u .i t I** -i #v. * ,.i 1 r -v ` K I. r . * A ' t A u .* t! 1, I i.| .V f J .1 \ *lt | ' , s ,*1-1 t, *.i'*.i 4 WI, ,V* 'J Ii! i A .*,:/! Ai,f), *v h jO, At C) |t,i mi-1mi ii*`\ xu.int y <i,f *. i .* f . .-ii ii.-.i ii ii i'if *# T'm.* im pUii'ii h . a I.: u- \ 6 30 (/> o m -o4