Document 7MZJbV250VqykbmjNK18eJ0ga

y ]-& & & & _ 0 7 % t MICROBIOS MICROTOX TOXICITY TEST TEST SUBSTANCE___________________________________________ _ Identity: Mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC203CF. Current information indicates it is a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fuorochemical component of the test sample. METHOD;__________________________________________________ Method: Microbios Microtox "BASIC" Procedure Test type: Acute static GLP: No Year Completed: 1991 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition. Exposure period: 30-minutes S tatistical m ethods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics' Reagent. Test conditions: Diluent: Millipore Milli-QTM water Test temperature: Not noted. Stock and test solution preparation: A primary 2000 mg/L stock solution was prepared by dissolving 200 mg of test substance in 100 ml_ of Millipore Milli-QTM water. The pH of the stock solution was 6.6. An osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL stock solution. Appearance of test solutions was noted as "clear, colorless". All test solutions were made by proportional dilutions. Exposure vessels: 4 mL cuvettes. Number of replicates: two Number of concentrations: Four plus a blank control Element basis: Percent light loss RESULTS_________________________ !__________________________ Nominal concentrations: Blank control, 125, 250, 500, and 1000 mg/L 001251 Element value: 5-minute EC50 = 470 (420 - 520) mg/L 15-minute EC50 = 360 (330 - 390) mg/L 30-mlnute EC50 = 280 (260 - 300) mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS___________________________ ___________________ The test substance 30-minute EC50 for Photobacterium phosphoreum was determined to be 280 mg/L with a 95% confidence interval of 260 to 300 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, MN, 55133 PAM QUALITY_______________________________________________ R eliability: Klimisch ranking = 2. Testing meets all criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_______________________________ ______________ This study was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number J1884-3,1991. OTHER______________________________________________________ Last changed: 6/28/00. 001252 BEST COPY AVAILABLE f<*,. -M''"'fh^ ' jg3g?c`*i^ *, . 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Xo/It 7/19791 ab ; >ri G-OBS >' 1 G-EST id il4* f,i* ' i**'1 . v*p l:' -. .-. " .y A ff > * ; -.'-'xV. ;>". 123.000 93.0/ 45.0 123.000 96.0/43.0 250.000 98.0/ 36.0 250.000 91.0/ 33.0 500.000 , 92.0/ 26.0 ' 300.000 . 84.0/ 23.0 7C1000.000 94.0/ 17.0 *,:firf8Wfe /.l ooo.ooo .- .- 98.0/ 17.0 '. * - |'JIi-B L A N K Bo/Bt- 99 / 57 & B L A N K RATIC1" 0.5738 0.190 0.285 0.567 0.497 1.037 1.103 -2.184 2.319 \prW ifr' , 1 EC 30 465.422 < 417.172 TO 519.253 > CS o * t7 (1 *0 -S*o) mg/i. . 0.240 ~ 0.240 0.507 0.507 1.075 1.075 2.277 2.277 \ \ r .-- <o(\6f-e6t-nete. )X/t BEST CQPY AVAli R *0.99110 SL OP E * 0.9071 INTERCEPT = +6 .1429 I k rL"vCi;f-lO.RE1S9.5 1 0.155 irto=: PROB. 100 1 +- V i-fl'j + V ` I 7mV, -| -S-*a| 001255 ?~c ' \ \ L * ' ." .- , *'r **- K '. \-:W" :A'-';. `-V ", - V . ; NICRQTQX(r) DATA S t - t E E T ^ Q Q ( F C - 2 0 3 C F L o t 101 13-min b k d . J 1884-3 7/19/91 *b P& 3 ...... .. ^ L E PA IR / `-'CDNC. 1 2 *'3 '4 V- ":;;S x '6 f- 7 -8 123.000 123.000 230.000 . 230.000 500.000 -,300/000 XI000.000 V-Z-xi o o o .ooo Io/It 93.0/ 30.0 96.0/ 29.0 98.0/ 23.0 91.0/ 22.0 92.0/ 15.0 . 84.0/ 14.0 94.0/ 9.0 98.0/ 10.0 B-OBS 0.253 0.338 0.722 0.671 1.478 1.424 3.220 ` 2.960 B-E8T 0.303 0.303 0.661 0.661 1.443 1.443 i 3.152 3.152 L c s & t') a d ---- HO uo 5i 7< 7S~ ' BLANK Bo/Bt 99 / 40 BLANK RATIO 0.4040 y^y-; (: EC 50 '/360.954 ( 334.846 r_ TO 389.097 3d> > " : (,) 6-obs T> (1 --+ 67---cbTsN R 0.99329 SLOPE 0.8792 INTERCEPT +5.8887/ X *73 Uo 76 C`v*p*' I-SMu IKZ7 ..V/ RESVsK\ P R 0 8 . 8----------------- - + t f.i safe??* t* tf'-* ,-La, * -J Rs?Sy:i', A.. ,vvo-fv .... 7' -/r< . ; f e i f e i r . ! . >.. t r a -, - - 001?56 Jm 001257 99632 SLOPE = 0.8431 . INTERCEPT = +5.6212