Document 7MNpwov2a4KL6D7rb36BjYM5o

M SLSlG - 0 2 6 0 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (ACARTIA TONSA) TEST SUBSTANCE______________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fiuorochemical component of the test sample. METHOD:____________________________________________________ Method: ISO/DIS 14669-1997: Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea). Type: Acute static GLP: No Year completed: 2000 Species: Acartia tonsa Supplier: Test organisms cultivated from eggs, supplied by the Marine Biological Laboratory, University of Copenhagen, Denmark. Analytical monitoring: Dissolved oxygen and pH Exposure period: 48-hours Test organism age: Large copepodites and adults. Statistical method: LC50 values calculated by PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). Calculation of NOEC is by single factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution water: Filtered natural seawater (34 ppt salinity) from an unpolluted site in Byfjord, Norway. Dilution water chemistry: Not given. Lighting: A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by diluting 1.0002 g of test substance in 1 L of water. Aliquots were then added directly to water in test vessels to create test solutions. Exposure vessels: 28 mL rolled rim vials containing 25 mL of test solution 002373 Number of replicates: 4 Number of organisms per replicate: 5 Number of concentrations: seven plus a blank control Water chemistry during the study: pH range (0-48 hours): 8 .2 - 8.2 (control exposure) 8.1 - 8.2 (1000 mg/L exposure) Temperature range: 20 1 C Dissolved oxygen range (0-48 hours): 7.3 - 7.4 mg/L (control exposure) 6.4 - 7.6 mg/L (1000 mg/L exposure) RESULTS_____________________ ___ _________ Nominal concentrations: Blank control, 32, 56,-100,180, 320, 560, and 1000 mg/L. Reference substance (3,5-dichlorophenol) at 1.0 mg/L. Element values: 24-hour LC50 = 610 (490 - 790) mg/L 48-hour LCso = 280 (220 - 360) mg/L 48-hour NOEC = 180 mg/L Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________ :_______________ The test sample 48-hour LC50was determined to be 280 mg/L with a 95% confidence interval of 220 to 360 mg/L. The 48-hour No Observed Effect Concentration (NOEC) was 180 mg/L. Validity criteria fulfilled and reference substance was in normal range. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_____________________________________________ _ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study_ lacks analytical confirmation of the amount of fluorochemical proportion in the solution. 00237' Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-4, 2000. O T H E R _____________________________________________________ Last changed: 6/26/00 002375 L99a3b1.r2e-f4.no.: Lage 1 of 31 (Q i NORSK AKKREDTTERING ROGALAND RESEARCH "FC-603 EF Light W ater TM ATC TM 3% " Toxicity tests Report RF-2000/004 Lab. ref. no.: 99312-4 No. of pages: 31 ISBN: Author Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 /1 4 .0 1 2000 Research Program: Open from (date): . Summary: The following test results were obtained with "FC-603 EF Light Water TMATC TM 3% ": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h EC, 48 h LCM 10 d LCM (m g/l): -170 (m g/l): 280 (mg/kg dry weight): >3060 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part of this docum ent m ay be reproduced in any form without perm ission in writing by RF - Rogaland Research . /S4 v jS tu d y id ire c to r Inger Lisa Andersen for RF - Rogaland Research Kre Netland 00237< 99312-4 CONTENTS: 1. Preface........................... ......................................................................................... l............ ;......3 2. Test for toxicity with Skeletonema costatum........................ .........................................4 3. Test for toxicity w h h Acartia tonsa.......................r".......................,...................... ........10 4. Test for toxicity with Corophium volutator..................................................................15 5. Datasheets.................................................................................................. .............................20 002377  L99a3b1.r2e-f4.no.: Page 3 of31 PREFACE: Ecotoxicological tests were performed on TC-603 EFLight WaterTMATC TM3%"in agreement with Novatech. The sample is a concentrate that normally will be used in a 3% AFFF/3% AR solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format"(OSPARCOM1995/S F T 1998). The project was carried out at RF - Rogaland Researchfor 3M Norge. All the tests were performed by RF - Milj0lab. Study director was Senior Engineer Inger Lisa Andersen. The test results are presented in separate sections. The results relate to the product as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and working proceduresfor all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes internal quality control schemes, participation in interlaboratory comparisons, and the use of certified reference materials and secondary reference materials. . The tests performed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without the permission in writing by RF - Milj0lab. 002378 L9a9b3.1r2e-f.4no.: Page 4 o f31 T est for T oxicity Marine alga: Skeletonema costatum 002379 L99ai3),1r2e-j4.no.: Luge J oj ) i TEST REPORT Grow alga tShkienlheitbointieomn taecstoswtaitthutmh e m a r in e fQ l NORSK Nr.RoilAKKREDITERING ROGALAND RESEARCH TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The 72 hours EC50was calculated to ~170 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: - METHOD: NwSit-hESNkeIlSeOton1e0m2a53c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-cMtylaurminetraiclgoamlugtruomw.th inhibition test TEST ORGANISM: SorkgealentiosnmemisakceopsttaattuRmF, -stRraoignaNlaInVdARBesAeaCrc1h.. A stock culture of the test PREPARATIONS: NaanCnsuaodtbtmruabiremsraniisltiesssffseolaiyarocwhncthoe.aaretedtgeridrn(ogstwaotltoiahnbIimStoyOue:td317i4u05m2%C5.o3)pT,frarhionoemdrwtroaaentcueoursmenw.pmaoTselhnlfuiedlttaeseedtriaoeswdnitsaetthfeirrnroomwBugyathfshjoeaenrPGdraiFcwr/hiCsaesdf.uilwsteeitrdh, Tmegs/1t swoalustpiornepsawreedrebpyrsetpirarriendgfr1o.0m20agsrtoamckssoofluthtieonpr. oAduscttoicnk1solilturteioonf gorfo1w0t2h0 medium. Other concentrations were prepared from this stock solution. INCUBATION: TolTaerhtbseetidtctaeutloslstthauwbarkeaossuerotr,u2f3n05r00ue0nmpdclileecrwlalscte/eormsneftlioinnfrrcuoeuomabucashatelncidgoehinxntpce(o1>nn0t0e5rna0mttipiolaEnflll/.yamtTg-2bre-oossewtttkcoi)numfglrteuoidnrmoefsclfaulwsulkueosrmreoe.nicsnucoaiemncnurte^^"t tubes of universal natural white type, at temperature of 20 2C. r Tbthyheeftlteuesostrtfewlascaseksnsrcueena.cfphoHrdaambyoefuaostru7gr2reomhwoetunhrtssm,waeanersduesrpueebmrsfeaonmrtmsp.elAedslagosafli5ngdmrioclwawttehedrweoantaskdmeanetaafssruhormeeedt. : .-t 002880 ' L99a3b.1r2e-f4.no.: Page 6 of 31 DATA ANALYSIS: CALCULATIONS: Gverroswusthtirmatee,saws dereesccrailbceudlaitnedNbSy-EloNgIlSinOea1r0r2e5g3r.ession on biomass (fluorescence) ErCso'. Tce3of2hfn0eecmceitnngcht/orli.bnaitctieoonrntyroeafftifaoepncrtwoodanustcchtaeglcgivuriolnawtgethd50bo%yf Sirnketedelurepctotoinolaentmiooanf cgboresottwwatteuhemnrailtOse eO(ExmprCgrfeslosas)-enddTahsisthe NOEC: CfcaoacnltccouerlnaAttriNoantOiooVnfAmNfOeoaElnloCww(ietNhdotbhOyebBcsooennrvtfreeordlrEomnfefiseacnpt.rCocoendcuernetrfoartiocnom) ips adroinnge ebaycshingle TEST RESULTS: COMMENTS: VALIDATION: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance W"FaCt-e6r0TM3 EAFTLCigTMht3%' EjC io - ErCso ErCso -170 100<ECso<320 NOEC: 100 VGVaaralluupeehssPianarddeiIccnaaStlectauctloa3nt.e0cd.enbtyraintitoenrps ogliavtiinogn.10N%O, E50C%araencda9lc0u%laitnehdibbyitiuosninogftghreowcothmrpauttee.r pUrongitrsainmmmge/1. The results are based on growth rates for the time span 0 - 72 h, the period d(suereinggrowwhtihchcuthrveegsr).owth is considered to be exponential in the control cultures Tc(3vcea,ho5lrelr-ydrdvbeieacsynhlpismdlooitnirotydyoriiepcnnhrtcghierttaeneonraoisala1ewgd,g0raibvousyweninnatithisftnahrdacetuthtoerreaironntogfegsfmetthmnpoeroorteroreemttsohttachaaolnalnylnd0af,1rot9eh6udefniun'd71l).2ft,hilthleheoetdue,crsostinnptettershrotaiolrtdeptshHueldtcsoidwntnirtohotl LTRSaeaemspbto.pprrleeterfd.rinaoetdoce:e.::ived.: 0170-21.900091..301.219202..990-9490 tX-L Wo An, Elin Horve geMolversmyr " Engineer Analytical technician SenQiouraRlietsyeAarscshurSacniceentis~t Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaulat bthoerapteorrmy iascsciorendiintewd rbiytinNgobrwyeRgFia-nMAiclcjdreladbit.ation (NA). The report may not be reproduced in any 002381 L99a3b.1r2e-f4.no.: L'ge 7 oj 31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: FC-603 EF Light Water TM ATC TM 3% Lab. ref.: 99312-4 Start date: 07.12.99 Cone, unit: mg/l Measurement: The biomass was measured by fluorescence; values in fluorescence units Time: 07.12.99 08.12.99 09.12.99 10.12.99 Concentration 14:50 09:30 10:25 11:55 Growth pH pH rate Corre- at start at end day-1 lation of test of test 0 0.12 0.26 0.93 4 1.22 0.998 .* 0 0.1 0.34 1.7 9.7 1.59 1.000 0 0.1 0.33 1.1 6 1.42 0.998 8.2 8.2 0 0.09 0.3 1.1 6.4 1.48 0.998 0 0.09 0.26 0.88 6 1.46 0.995 0 0.09 0.26 0.9 7 1.51 0.994 3.2 0.09 0.43 1.75 11 -'1.67 0.998 3.2 0.09 0.28 1.2 6.3 1.48 1.000 8.3 8.2 3.2 0.08 0.23 1 4.7 1.42 1.000 10 0.08 0.4 3.4 14.5 1.81 0.995 10 0.09 0.4 2 11.5 1.69 0.999 8.3 8.3 10 0.1 0.4 2.2 12 1.66 1.000 32 0.1 0.28 2.05 9.3 1.57 0.998 32 0.1 0.3 1.9 9.2 1.57 0.999 8.3 8.2 32 0.11 0.3 1.65 8.8 1.52 0.999 100 0.09 0.31 0.77 5.4 1.42 0.991 100 0.11 0.35 0.75 5.1 1.33 0.986 8.3 8.2 100 0.1 0.36 0.78 5.4 1.39 0.987 320 0.07 0.04 0.03 0.04 0.00 320 0.09 .. 0.03 0.01 0.04 0.00 320 0.06 0.04 0.02 0.06 0.00 8.3 8.2 1000 1000 1000 0.09 0.07 0.08 0.05 0.09 0.03 0.03 0.05 0.1 0.03 0.03 0.02 0.00 0.00 0.00 8.3 8.2 Reference substance (3,5-dichlorophenol): 1.5 mg/l 1.5 mg/l 0.09 0.15 0.15 0.08 0.1 0.12 1.5 mg/l 0.08 0.14 0.18 Mean values: 0 3.2 10 32 100 320 1000 0.10 0.09 0.09 0.10 0.10 0.07 0.09 Reference subst. 0.08 0.3 0.3 0.4 0.3 0.3 0.0 0.0 0.13 1 1 3 2 1 0 0 0.2 0.2 0.22 0.2 7 7 13 9 5 0 0 0.2 0.28 0.908 0.35 0.968 0.32 0.921 1.45 1.52 1.72 1.56 1.38 0.00 0.00 0.32 0.998 0.999 0.999 0.999 0.988 0.969 The result of the reference substance (22% inhibition) is within the range 002382normally found (20-80% ) L9a9b3.1r2e-f4.no.: Page 6' oj 31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: FC-603 EF Light W ater TM ATC TM 3% Lab. ref.: 99312-4 7 -, 5- 3S 31 o ?gCO 1 -1 09 1000 mg/I 24 48 72 ItUBSJjt LlUIT^Uu ttw urstg 7 -| | 5 3 3 OS 81 ?.10 -3 320 mg/l 24 48 72 test duration (hourg)* 002383 L9a9b3.1r2e-f4.no.: Page 9 of31 Inhibition of the growth of Skeletonema costatum with: mg/1 ( FC-603 EF Light Water TM ATC TM 3%) Experimental data: Concentration in Growth rate FC-603 EF Light Water TM ATC TM 3% Control 1 Control 2 . Control 3 Control 4 Control 5 Control 6 Control mean : 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 320.00 320.00 320.00 1000.00 1000.00 1000.00 1.22 1.59 1.42 1.48 1.46 1.51 1.45 1.67 1.48 1.42 1.81 1.69 1.66 1.57 1.57 1.52 1.42 1.33 1.39 0.00 0.00 0.00 0.00 0.00 0.00 Inhibitic in percer - 0 0.0 0.0 1.8 0.0 0.0 0.0 0.0 0.0 0.0 1.8 8.1 3.9 100.0 100.0 100.0 100.0 100.0 100.0 002384 L9a9b3.1r2e-f4.no.: Page 10 o f31 T est for T oxicity Marine herbivore: Acartia tonsa 002385 L99a3b1.r2e-f4.no.: Page 11 of 31 TEST REPORT ACUTE TOXICITY TEST WITH Acortiam a r ine co pepo d TtoHnEsa TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The 48 hours LC50 was calculated to 280 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ItoSOm/aDrIiSne1c4o6p6e9p-od1s99(C7:oWpeaptoedraq,uCarliutysta-cDeaet)e. rmination of acute lethal toxicity TEST ORGANISM: Acartia tonsa University of C(Doapnenah),asguepnp. lied by the Marine Biological Laboratory, CULTIVATION: The test organisms were supplied as eggs, and were cultivated in accordance with the "Paris Commission - Ring Test Protocol" (VKI). Large copepodites and adults were used in the test. PREPARATIONS: Npasraibtouarrstaiosl usfeosarew.thaetedril(usatiloinnitwy:at3e4r.%To)hferowmataernwunaspofillltuetreeddstihteroiungBhyawGorFd/Cwafisltuersed Test solutions were prepared from a stock solution. A stock solution of 1000 mwagt/e1rw. aOstphreerpcaornedcebnytrsattiriorninsgw1e.0re00p2regpraarmedsforof mthethpirsosdtuocctkinsol1ultiitorne.of dilution T2v5ihaemlstlewsoetfrwteeakssterpsuotnluunitnidoegnrlaaasns1dc65ohn/a8tnahiimnliegarhlsst/,(d24a8rrkemppllihrcooatltloeepsdefrroiirmodeavacitahalsc)toenemacpcehnertcraoatuntirtoaenion. fiTnghe 20 1 C. pH and oxygen measurements were performed as indicated on the data sheet. ~ 002386 L99a3b1.r2e-f4.no.: Page 12 o f31 DATA ANALYSIS: Calculations: The effect on the survival of Acartia tonsa is expressed as the concentration of paeprrtoedsut cctolnecthenatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLioCn5.0)T. hTeh2e4cohmoubrisnaenddm4o8rhtaoluitrys LstCan50davradlupersoacenddutrheeP9R5%OBcIoTnAfidneanlycseisli,mNiattwioenrael cSawlceudlaisthedEbnyviurosinnmgetnhteal Protection Board (Version 2.3, 1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single fcaocntcoernAtrNatOioVnAmfeoalnlowwiethd tbhyeBcoonntfreorrl omneisanp.rocedure for comparing each TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-603 EF Light Water TMATCTM3%". Acartia tonsa 24 hours 48 hours LCio 280 (130-380) 89 (54 -120) LC50 610 (490-790) 280 (220 - 360) LC90 > 10 0 0 880 (620-1500) NOEC: 320 180 ctVhaaellcucueolsmaitpnedudtibecyraptPerRo1gO0r%Ba,mIT5m0a%enaGalyrnsadips,9h0aP%naddl9eInt5hS%atalcittoy3n.a0fif.dteern2c4e ianntedr4v8alhsoaurres.indUicnaittsedin. mNgO/LECVaarlueecsaalcreulated by V a l id a t io n : The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the testwas greater than or equal to 4 mg/1, the percentage mortality in the controls was less than 10%, and the 48 hours LC50 of 3,5-dichlorophenol (reference) was in the range normally found. Lab. ref. no.: Sample received.: Test period: Report date: 99312-4 1007-2.10021..0.12910..990990 Elin Horve ge Molverrscmrrwyrrr AnalyEticnaglinteecehrnician SenQiouraRlietsyeAarscshurSacniceentist Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaulatbthoeraptoerrmy iascsciorendiintedwrbiytinNgobrwyeRgFia-nMAiclcjpreladbit.ation (NA). The report may not be reproduced in any 002387 L99a3b1.r2e-f4.no.: Page 13 of31 PRIMARY DATA: Acute toxicity test with Acartia tonsa Test substance: Lab. ref.: Start date: Measurement: "FC-603 EF Light W ater TM ATC TM 3%" 99312-4 10.11.99 Number of dead Concentration (mg/l) Control 32 56 100 180 320 560 1000 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 5 56 30 4 5 5 5 55 20 5 5 55 20 5 555 20 5 5 55 20 5 5 65 20 4 6 54 21 6 555 20 5 NUMBER OF DEAD 24 hours 48 hours A CE B DF Total A CE BDF Total 0 00 001 0 00 0 0 00 1 0 00 0 0 00 00 0 0 00 0 0 01 00 1 0 00 1 1 11 11 4 0 00 0 0 11 1 2 13 3 4 54 3 2 12 01 0 24 32 5 33 95 5 55 16 5 6 8 16 20 Reference substance (3,5-dichlorophenol): 1.0 mg/l 5 55 1 10 331 30 6 45 0 01 3 0 11 9 The result with the reference substance is within the range normally found. u St mt 002388 L9a9b3.1r2e-f4.no.: Page 14 of31 Test substance: "FC-603 EF Light W ater TM ATC TM 3%" Lab. ref.: Start date: 99312-4 10.11.99 Measurements o f oxygen and pH. Concentration (mg/l) Start Oxygen (mg/l) pH 24 hours 48 hours Oxygen pH Oxygen (mg/i) (mg/l) Control 7.3 8.2 7.4 32 7.6 8.1 7.1 56 7.7 8.1 7.1 100 7.8 8.2 7.1 180 7.7 8.2 6.8 320 7.7 8.2 6.8 560 7.6 8.2 6.5 1000 7.6 8.2 6.4 Reference substance (3,5-dichlorophenol): 1.0 mg/l 7.3 8.2 7.4 pH 8.2 8.2 8.2 8.2 8.2 8.2 8.1 8.1 8.2 002389 L9a9b3.1r2e-f4.no.: Page 15 o f31 Test for toxicity Marine Amphipode: Corophium volutator 002390 L9a9b3.1r2e-f4.no.: Page 6 o f31 TEST REPORT ACUTE TOXICITY SEDIMENT TEST WITH THE MARINE AMPHIPOD C o w p h i u m V O lutO tO T TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The lOd LC50 was calculated to >3060 mg kg*1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ASTM E1367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments. TESTorganism: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: Tafeeheredatitenedgst.soeragwaantiesrmast w15erCe .suAppslmieadllaasmadouulntst o(4f-n8amtumralinsesaiwzee)eadnwdawseurseehdeflodrin PREPARATION: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjorden outside wStaasvaunsegderfowrapsruepseadrifnogrtdeisltusteiodnimweantetsr.. TShedeismedenimt ceonltleisctaedmautdadnyusannpdolaluntdedwsaiste analysed for organic content and proportion silt for each test. bEd4a0uam0cckhpmettselewsodtaficmssoesneahncwaetkanitneternraaftwpoiorlanas3swtahidcaodsbuepurdsrc.etkpoTeatehr.aeecd1hs5be,0dyaimnmaddledtonhiftneswgbeaatehwsaekdapetirversriodwdweuedacrstienainatdoed6rtea0hdt0reeadgenrodbavmeteahrskeoerfs, ntchaigelchduta.lamt1ep0dsaaendsimimmagelnsktgw*we1arsesedathinmeanleynrsatendddrfoyomrwlweyiagstheeltre. cctoendtefnort, eaancdhablel ackoenrc.enAtrsaatmionpsleaoref INCUBATION: The test was run in glass containers (600 ml) each containing 200 g o f test sceodnicmenetnrta,taiopnp.. 4T0h0embelaokfesresawwearteerkaenpdt u1n0daenricmonaltsin, 3uoruesplaicearatetisofnoarteaach temperature o f 15 1C. pH and oxygen measurements were performed as indicated on the data sheet. 002391 L99a3b1.r2e-f4.no.: Page 17 of 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Corophium volutator is expressed as the concen mtvraaolturioteanlaiotnydf patehprerto9ed5s%ut cctocnloecntehfniadtlretanotci5oe0nl%iwmeoirtfeatrhueesectdaelsfctouarlnactiameldcaublslya(tLuiosCnin5.0g)T.thhTeehs1et0acndodamayrbdLinCe5d0 PROBIT Analysis, National Swedish Environmental Protection Board (Version 920.3%,19o9f t0h)e. tTeshtisanpirmogarlsa.mme also calculate test concentrations lethal tp 10% and NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single fcaocntcoernAtrNatOioVnAmfeoalnlowwiethd tbhyeBcoonntfreorlromneisanp.rocedure for comparing each Test RESULTS: Table 1. LC and NOEC values obtained with "FC-603 EF LightWaterTMATCTM3%" Corophium volutator 10 days LCio 99 ( -760) LC >3060 LC*, >3060 NOEC: 3060 cVVaaalllcuuueelssaaitnerddeibccyaatltechue1l0ac%otem,d5pb0uy%tePraRpnOrdoBg9Ir0Ta%manmlaetelhyGsailsri,tayapanhfdPtea9rd51%I0nScdotaanytfsi3.d.0eUn. cneitsinintermvgalksga''reseidnidmiceanttedd.ryNwOeEigChta. re VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved otmfosshoeexbodredysriiegopmmtrreaevgnreleieacnntdcyentton)iwaicnntbacascugaiesogtnenrnt1tmehtr6ieffae.eino7tcrmr%tiaeotbnnano.ulctriatettTmyattshhltouiihnteerbyetsstsaiehietnlldaniettnitdyfmchcroioeeanenfcnhottotrtiifhgonou2ehnelss0eteci.eswsd9ostnetrimcrsecwoecegnfonaolcFmsetuerslgnnuamsrdttoetrierhoaatnaonattdneinobr(et1nhde1t0he5wwh6na%i0neaigtm.shhkogineTrgn*rohe1.ktaqedgcWsurpic'ga1yeeelndprwtihcrtfoeyeeaicdniv6wgate0lhaneimgtit%g.eih,tst Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0 .8 Lab. ref. no.: TSRaeemsptopprleterdrioaetdce:e:ived.: 99312-4 20.09.99 0-10.12.99 07:01.2000 Enclosed: Datasheets EElinngHinoerevre Analytical technician SeniorgRe eMseoalrvcehrsSmcyiernytist Quality Assurance 002392 L9a9b3.1r2e-f4.no.: Page 8 o f 31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-603 EF Light Water TM ATC TM 3%" Lab. ref.: Start date: Measurement: 99312-4 17.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 10 10 10 222 30 10 10 10 457 30 1060 10 10 10 30 3060 10 10 10 30 \ \ NUMBER OF DEAD 5 days 10 days ACE B DF Total ACE BDF Total 0 00 0 00 0 0 12 3 2 02 4 232 7 3 20 5 r Reference substance (fluoranthene): j 10 10 10 20.9 30 j 1 122 5 002393 L9a9b3.1r2e-f4.no.: Page 19 o f31 Test substance: "FC-603 EF Light Water TM ATC TM 3%" Lab. ref.: Start date: 99312-4 17.12.99 Measurements o f oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH Control 94 8.1 5 days Oxygen (%) pH 90 $4 222 96 8.1 92 8.1 457 97 8.1 93 8.1 1060 92 8.0 62 7.7 3060 100 8.1 93 8.0 10 days Oxygen (%) pH 97 8.1 94 8.0 97 8.1 96 8.1 96 8.1 Reference substance (fluoranthene): 20.9 95 8.1 92 8.1 83 8.0 002394 L99a3b1.r2e-f4.no.: Page 20 o f31 D ata Sheets 002395 L9a93b.1r2e-f4.no.: Page 21 o f31 HMS-DATA3LAD (Heise-, Miljo- og Sikkerhetsdatablad) Dokumentr. : Erscatter : 07--7297--0 Utgitt s 03/11/93 Versjon : 03.00 24/04/97 Skrevet ut : 11/11/98 Side 1 av n HMS-databladet er utarbeidet av Miljeavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIERSJON AV KJEMIXALIST OG ANSVARLIG FIRMA-. Importer: 3M Norge A/S Handels r.avn: FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Deklarasjonsnummer, Produktregisteret: Telefon, Gifcinformas jor.ssentralen: 22 59 13 00 2 OPPLYSNINGER OM KJEMISX SAMMENSETNING. Informasjon om enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VSXT-PROSENT VANN 7732-18-5 1,2-STANDI0L 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 2-(2-3UTOKSYETOKSY)-STANOL / 112-34-5 (DIETYLENGLYKOLMONOBUTYLETER) Faresymboler/risikosetninger: Xi; R: 36 SYNTETISK OVERFLATEAKTIVT STOFF Trade Secret FLUORALKYL OVERFLATEAXTIVE STOFFER . Trade Secret FLUORKJEMISK AKRYLAT Trade Secret TRIETANOLAMIN * 102-71-6 TYKNERE Trade Secret RESTER AV ORGANISES FLUORKJEMIKALIER Trade Secret 52 -- 56 - 22.5 - 10 4-6 1- 5 1- 3 a1 1- 3 Ikke kjent 002396 L9a9b3.1r2eJ-4.no.: Page 22 oj 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF BEST COPY AVAILABLE 3 VIXTIGSTZ FAREMOMENTER. Vikcigste skadevirkninger Eelsefare: Kan vasre farlig ved svelging. Kan irritere aynene. Sikkerhec: Miljafare: Spesialavfall. 4 FORSTESJSLPSTILTAK. Tiltak ayne: Skyll straks aynene med rikelige mengder vann. Kontakt lege umiddelbart. Tiltak hud: Skyll straks huden med s&pe og rikelige mengder vann. rjern tilsalt tay. Evis symptomer oppstar, kor.takt lege. Vask tilsalt tay far det brukes igjer. og kast tilsalt skotey. Tiltak innanding: Evis symptomer oppstir, bring vedkommende bort fra ekspoheringskilden. Sarg for frisk luft. Svis symptomene vedvarer, kontakt lege. I Tiltak svelging: Kontakt lege umiddelbarz. Fremkall brekr.inger kun i samrid med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstlas person. : j ! 5 TILTAX VED BRANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: 02.i97 L9a9b3.1r2e-f4.no.: Page 23 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF BEST COPY AVAILABLE Forbrenningsprodukter ved brann: Prosedyrar ved siukkir.g av brann: 6 TILTAK VED UTILSIXTET UTSLIPP. Personlige vernetiltak: __ Se vernetiltak under andre punkter i dette HMS-databladet. Tiltak ved utilsiktet utslipp: Ventiler omridet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale. Spill sanies opp umiddelbart. Rengjer omridet med vann. Plasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfall: Se punkt 13, Fjerning av kjemikalieavfall. 7 ENDTSRING OG OPP3EVARIMG. if Betingelser for sikker oppbevaring: Ma ikke lagres i omrider hvor produktet kan komme i kontakt med mat eller legemidler. Lagres ved temperatur under 49 grader Celsius. Lagres ved temperaturer over 0 grader Celsius. Hold beholderen tett lukket nir produktet ikke er i bruk. Uforenelige materialer: Ventilasjon: Oppbevar emballasjen pa et godt ventilert sted. Sikkerhetstiltak for i forhindre brann: Sikkerhetstiltak for i forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for i forhindre statisk elektrisitet: Forholdsregler ved bruk: Hold emballasjen tett lukket. Reyking forbudt: Under bruk av o o 2398 produktet kan reyking for&rsake forurensning av tobakken og/eller L9a93b.1r2e-f4.no.: Page 24 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF 8 EKSPONERINGSKONTROLL OG PERSONLIG VSRNEUTSTYR. yevern: Unnga & fk damp, spray eller tike i eynene. Bruk tette vernebriller med ventiler. Hindvern: Bruk egnede vernehansker av hensiktsniessig materiale ved hlndtering av produktet. Vernehansker av felgende materiale(r) anbefales: Butylgummi. Annet hudvern enn hancvern: Unngl hudkontakt. Andedrattsvern: Unr.gi inninding av luftb&rer materiale. 3asert pi konsentrasjonen av luftforurensningar i arbeidsatmosfaren velges en av felgende type(r) godkjent incedrettsvern: halvmaske med filter mot organiske daaper og vaskeforaige partikler: A/P2SL. Svelging: Det mk ikke spises, drikkes eller roykas under arbeid med procuktet. Vask utsatte omracer grundig med s&pe og vann. Vask henden'e etter hanctering og fer spising. Anbefalt ventilasjon: Hvis utsugningsventiiasjon ikke er tilstrekkelig, m& egnet godkjent indedrettsvern benyttes. Sorg for tilstrekkelig ventilasjon slik at dampkor.sentrasjonene holdes under anbefalte administrative normer og/elier kontrolier spray eller t&ke. Ada. normer for forurer.sning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger-: Ingen adm. norm. 1 f2-ETANDIOL (107-21-1) 8-timers verdi ppn: - (stev) 8-timers verdi mg/m3: 10 (srev) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) 2 -(2-BUT0KSYET0KSY)-ETANOL / (DIETYLENGLYKOLMONOBUTYLETER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). SYNTETISK OVERFLATEAXTIVT STOFF (Trade Secret) Anmerkninger: Ingen adm. norm. V* t t ^ w r 002399 L9a9b3.1r2e-f.4no.: Page 25 o[31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF -FSSe raJZMUa AXRYLAT (Trade-Oeu>ei.)-------- -- ------------------ -- ---Anmerkninger: Ingen adm. norm. TRIETANOLAMIN (102-71-6) 8-timers verdi ppra: 8-timers verdi mg/m3: 5 TYKNERS (Trade Secret) Antnerkninger: Ingen adm. norm. RESTER AV ORGANISKE FLUORXJEMIKALIER (Trade Secret) 8-timers verdi mg/m3: 0,1Grenseverdi anbefalt av 3M. ! ' ;| I j i 9 FYSISKS OG KJEMISKS EG2NSXAPER. Tilstandsform, farge, lukt: Gul/brun vaske. IIi I \ pH; 7.5 - 8.5 Xokepunkt/-Intervall: Smeltepunkt/-intervall: < 150 C Ikke relevant ; i ; Fiammepunkt: Ingen (Setaflash) i Selvantennelighet: Ikke kjent Sksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, avre: "* Ikke relevant ! Damptrykk: ca. 15.8 mmHg (Kalkulert v/20C) Laselighet i vann-: Spesifikk vekt: Fullstendig * ca. 1.06 (vann=l) Damptetthet: ca. 0.88 (luft*l) (Kalkulert V/20C) Flyktige organiske forbindelser (VOC): - 355 g/1 SCAQMD (Kalkulert v/20C). Fordampningshastighet: Ikke kjent Viskositet: < 5000 centipoise 002400 L9a9b3.1r2e-f4.no.: Page 26 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF IO STABILITT OG REAKTIVITT. Forhold som skal unngis: Materialer som skal unngis: Ikke kjent. Farlige spaltningsprodukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller partikler. Dekomponering av bruksferdig blanding forventes ikke i representere noen risiko. Stabilitt og reaktivitet: Stabilt. Farlig polymerisasjon vil ikke forekomme. j ! t { II OPPLYSNINGER OM EZLSEFAR2. Sffekrer avne: Mild ayeirritasjon: symptomer kan vare radhet, hevelse, smerter og tirar. j j Effekter hud: Mild hudirritasjon .-(etter langvarig eller gjentatt kontakt): symptomer kan vare radhet, hevelse og klae. Langvarig eller gjentatt eksponering kan fare til: Allercisk hudreaksjon: symptomer kan vare rcdhet, hevelse, blemmer og klae. Effekter inninding: En enkelt overeksponering somoverstiger anbefalteadministrative normer, kan fare til: j j Pvirkning av sentralnervesystemet: symptomer kan vare hodepine, arhet, savnighet, manglende koordinsjonsevne, nedsatt reaksjonsevne, slaret tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon (avre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsek utfart med organiske fluorkjemikalier, som finnes i dette produktet, visereffekter somleverforstyrrelser, vekttap, i : manglende appetitt, slavhet samt effekter som pivirker neurologi,? I bukspytt, binyre og hematologi. Det foreliooer v-s-** L9a9b3.1r2e-f4.no.: P a g e l! o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF veiledning. Svelging kan fare til: Pivirkning av nyrene: symptomer kan vare redusert urinmengde, bld i uriner, og ryggsmerter. Aspirasjonspneumoni (Lungebetennelse sota skyldes at fremmedlegemer kommer ned i lungene. En alminnelig irsak til tilstanden er oppkast.): sytaptomer kan vare hosting, pustevansker, gisping, hoste opo bld og lungebetennelse sota kan vare livstruende. Informasjon ota allergi, overfelsomhet: Kreftfremkallende egenskaper: Arvastoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dette produktet inneholder en eller fiere organiske fluorkjemikalier, som muligens kan absorberes og lagres i kroppen over lengre tid, enter, som utgangsmolekylet eller som nedbrytningsstoffer (metabolitter), og kan akkumuleres ved gjentatze ekspcneringer. Det foreligger ingen kjente helseeffekter for mennesker fra-eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Forekomszen av organiske fluorkjemikalier i blodet til den generelle befoikningen og underpopulasjoner som arbeidere, har vart publisert siden 1970 irene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. 12 OPPLYSNINGEA OM MILJ0 FARE. Persistens/Biologisk nedbrytbarhet: Dette stoffet er ikke lett biologisk nedbrytbart, men brytes delvis ned i tester for lett biologisk nedbrytbarhet. Dette produktet inneholder en eller fiere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljoet (persistente). Okotoksikologiske data: 002402 mDect/ 1te< stlaovf<fec!t-o err r ^mninim=-af~lctn gi. futi--g or -veerfo-r akvatiske organismer (100 L9a9b3.1r2e-f.4no.: Page 28 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Ancre skacevirkninger, miljo: Data ora biologisk r.edbrytbarhet: COD (Chemical Oxygen Demand): 740000 mg/kg; BOD 5 dagers (Biochemical Oxygen Demand): 216000 mg/kg; 30D 10 dagers: 376000 mg/kg; BOD 20 dagers: 446000 mg/kg; BOD 28 dagers: 602000 mg/kg. Akvatisk giftighet: 96 timers EC50 for fisk (Fathead Minnow (Pimephales promelas)): > 1000 mg/1; 48 timers EC50 daphnia (Water Flea (Daphnia--magna)): 480 mg/1; 30 mir.utters EC50 Microtox: 124 mg/1;24 timers-EC50 Alge (Selenastrura capricornutum)): 170 mg/1. Behandling i renseanlegg: 3 timers EC50, Activated Sludge Respiration Inhibition: > 1000 mg/I. Sarg for avfallsh&nctering iht. gjelde.nde forskrifter. 13 FJSRNING AV XJEMIKALIEAVFALL. Metoder for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behandlingsanlegg for spesialavfall. Anbefalte avfallsgrupper: HOVEDKATEGORI 07: AVFALL FRA ORGANISES PROSES5SR. UNDERRATEGORI 07 06: Avfall fra P3DB av fettstoffer, slpe, rengjoringsmidler, desinfeksjonsmidler og kosraetikk. EAX-kode 07 06 03: Halogenerte organiske lesemidler, vaskevasker og moderlut. Avfallstoffnummer: 7151 Organisk avfall med halogen. Spesielle komponenter: inneholder fluorforbindelser. ,, 14 OPPLYSNINGER OM TRANSPORT. ADR-klasse: siffer: .- 0024C 3 L9a9b3.1r2e-f4.no.: Page 29 of 31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF bokstav: margr.ummer: CEFIC-kor" : ITN-nummer: ICAO-klassa: IMDG-klasse: IMDG-sica: EMS-r.r. : MFAG: Ovrige opplysninger: Ikke transportfarlig gods. 15 OPPLYSNINGER OM LOVER OG FORSXRIFTER. Merkeetikett: Symbol{er): KSLSESXADELIG (Xn) Ir.neholder: Etyler.glykol 22,5 %, 2-(2-butoksyetoksy)-etanol 10 %, Trietanolamin 1% R-setninger: Farlig ved svelging. S-setninger: Unng. inn&ning av damp/spraytetike. Unngi kontakt med huden og eynene. Fir man Stoffet i oynene; skyll straks grundig med stre mengder vann og kontakt lege. Fir man Stoff pi. huden, vaskes straks med stre mengder s&pe og vann. Ved svelging, kontakt lege omgiende og vis denne beholderen eller etiketten. YL-gruppe: 00 Korr, yrkeshyg. luftbehov: 002404 Ovrige opplysninger om merkeetiketten: Ovennevnte merking er basert p& tidligere merkeforskrifter og vil - - fl L9a9b3.1r2e-f.4no.: Page 30 of31 FC-603EF LIGHT WATER (TM) ATC 31 AR-AFFF reviderte merkeforskrifter av jar.uar 1998. Symbol: Inger. Ir.neholder : 1,2-etandiol, 2-(2-butoksyetoksy)-etanol, Trietanolarain Xan vare farlig ved svelging. Kan irritere oynene. Unng innr.ding av damo/spraytetke. Unng. kontakt med huden og aynene. Fr man stoffet i eynene; skyll straks grundig med store mengder vanr. og kor.takt lege. F&r man stoff pi huden, vaskes straks med store mengder spe og var.n. Ved svelging, kontakt lege omgende og vis denne beholderen eller etiketten. YL-gruope: 00 - Spesielle lover og bestemmelser: 1. Forskrift om utarbeidelse og distribusjon av Kelse-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking m.v. av stoff som kan mediare miljafare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfall. 6'. Etc. 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR BRUKSRNES SIKXSRHET OG HELSE. Anvendelsesomrder: Filmdannende skumkonsentrat for slukking av brann. Viktigste kide(r) ved utarbeidelsen av HMS-databladet: Datablad fra- 3M General Offices, St. Paul, Minnesot^, USA. rsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egne opplysninger: Informasjonen p dette HMS-databladet er i henhold til vire nvaerence opplysninger og beste oppfatning angende riktig bruk og hndteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medfarer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. 002405 L9a9b3.1r2e-f.4no.: Page 31 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF FOLGENDE PRODUKTES. DEKXSS AV DETTE HMS-DATABLADET: 3M ID-nr : XF-0008-0223-1 3M AFFF/ATC FC-603EF Brannslukn ingsskum Produksjon 3M ID-nr : XF-0008-0209-0 3M Light Water3/3%AFFF/ATC Fire Fighting Foam, FC-603EF,1000 L 3M ID-nr : XF-0008-0208-2 3M Light Water3/3%AFFF/ATC Fire Fighting Foam, FC-603EF, 200 L 3M ID-nr : XF-0008-0207-4 3M Light Water3/3 %AFFF/ATC Fire Fighting Foam, FC-603EF, 20 L A /U 2 6 - Z Gl ACUTE TOXICITY TO AQUATIC INVERTEBRATES (COROPHIUM VOLUTATOR) TEST SUBSTANCE___________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:___________________________________________________ Method: ASTM E1367-90, Standard guide for conducting 10 day static sediment toxicity tests with marine and estuarine sediments. Type: Acute static GLP: No Year completed: 2000 Species: Corophium volutator Supplier: Field collected from an unpolluted site near Trondheim, Norway. Analytical monitoring: pH and dissolved oxygen. Exposure period: 10 days Test organism age: Adults (4-8 mm size) Statistical method: LC50 values calculated by PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). Calculation of NOEC by single-factor ANOVA followed by Bonferronis procedure. Test conditions: Dilution water: Natural seawater (34 ppt salinity) from an unpolluted site in Byfjorden outside of Stavanger, Norway. Sediment collected at an unpolluted site. The sediment is a muddy sand and had an organic fraction of 0.7% and a silt fraction of 0.8%. Dilution water chemistry: Not given. Lighting: Not given. Stock and test solution preparation: Each test concentration was prepared by adding the test substance to 600 g damp sediment and 150 mL of seawater, then shaken for 3 hours. The sediment was split into three subsamples, with an overlay of 400 mL seawater added to each, and aerated overnight before study initiation. 002407 Exposure vessels: 600 mL glass beakers containing 200 g of test sediment and -400 mL of seawater with continuous aeration. Number of replicates: 3 Number of organisms per replicate: 10 Number of concentrations: four plus a blank control Water chemistry during the study: pH range (0*10 days): 8 .1 - 8 .1 (control exposure) 8.0 - 8.1 (3060 mg/kg exposure) Temperature range: 15 1 C Dissolved oxygen range (0-10 days): 90 - 97% (control exposure) 93 - 100% (3060 mg/kg exposure) RESULTS__________________________________ Nominal concentrations: Blank control, 222, 457,1060, and 3060 mg/kg (dry weight). Reference substance (fluoranthene) at 20.9 mg/kg dry weight. Element values: 10-day LC50 = >3060 mg/kg (sediment dry weight) 10-day NOEC = 3060 mg/kg (sediment dry weight) Element values based on nominal concentrations Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS ____________________________________________ The test sample 1 0 -day LC50 was determined to be >3060 mg/kg (sediment dry weight). The 10-day No Observed Effect Concentration (NOEC) was 3060 mg/kg (sediment dry weight). The 3060 concentration was the highest concentration tested and caused -17% mortality. Validity criteria fulfilled and reference substance obtained mortality of 16.7%. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY__________________________________________ _ _ Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. 002408 REFERENCES Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-4, 2000. OTHER_______________________________________________ Last changed: 6/26/00 002409 L99a3b1.r2e-f4.no.: Lage 1 of 31 4 (Q l NORSK AKKREDTTERING Nr. POT ROGALAND RESEARCH "FC-603 EF Light Water TMATC TM 3%" Toxicity tests Report RF-2000/004 Lab. ref. no.: 99312-4 No. of pages: 31 ISBN: Author Elin Horve Client(s): 3M Norge Distribution restriction: Version No. / date: Vers. 1 /14.01 2000 Research Program: Open from (date): . Summary: The following test results were obtained with "FC-603 EF Light W ater1,1ATC TM 3% ": Toxicity: Skeletonema costatum Acartia tonsa Corophium volutator 72 h ECm 48 h LCm 10 d LCm (m g/l): -170 (m g/l): 280 (mg/kg dry w eight): >3060 The results relate to the product as supplied by Novatech. Key-words: Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. No part o f this document may be reproduced in any form without perm ission in writing by R F - Rogaland Research . I\\A Inger Lisa Andersen for R F - Rogaland Research Kre Netland 002410 99312-4 CONTENTS: 1. Pr e f a c e ................................;..................................................................................................................................3 2. Test for toxicity with Skeletonema costatum......... ................:..............................................4 3. T est fo r to x icity wtthacartia tonsa..... .......................-1.................... ................................10 4. Test for toxicity with Corophium volutator.......... .......................................................15 5. D a ta sheets.............................................................................................................. ................................ 20 002411 L99a3b1.r2e-f4.no.: Page 3 of31 PREFACE: Ecotoxicological tests were performed on "FC-603 EFLight WatermATCm3%" in agreement with Novatech. The sample is a concentrate that normally will be used in a 3% AFFF/3% AR solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format" (OSPARCOM1995/S F T 1998). The project was carried out at RF - Rogaland Researchfor 3M Norge. All the tests were performed by RF - Milj0lab. Study director was Senior Engineer Inger Lisa Andersen. The test results are presented in separate sections. The results relate to the product as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and working proceduresfor all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes internal quality control schemes, participation in interlaboratory comparisons, and the use of certified reference materials and secondary reference materials. . The tests performed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without the permission in writing by RF - Milj0lab. 002412 L9a93b1.r2e-f4.no.: Page 4 of31 T est for T oxicity Marine alga: Skeletonema costatum 002413 L99a3u1.r2e-j4.no.: Luge U ojjj TEST REPORT Grow alga tShkienlheitbointieomn atecstoswtaitthutmh e m a r in e (0 1 NORSK AKKREDtTERING Nr. m i ROGALAND RESEARCH TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The 72 hours EC50was calculated to ~ 170 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: Method: NwSit-hESNkeIlSeOton1e0m2a53c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-cMtylaurminetraiclgoamlugtruomw.th inhibition test TEST ORGANISM: Skeletonema costatum, organism is kept at RF -stRraoignaNlaInVdARBesAeaCrc1h.. A stock culture of the test urej PREPARATIONS: NaaCnnsuaodtbtmruabiremsraniisltiesssffseolaiyaorcwnhctho.eaaertedtgerirdn(ogstwaotltoiahnbIimStoyOue:td317i4u05m2%C5.o3)pT,frarhionoemdrwtroaaentcueoursmenw.mpaoTselhnlfuiedlttaesetderiaoeswnditsaetthfeirrnroomwuBgyathfshjoaeenrPGdraiFcwr/hiCsaesdf.uilwsteeitrdh, Test solutions were prepared from a stock solution. A stock solution of 1020 mg/1 was prepared by stirring 1.020 grams of the product in 1 litre of growth medium. Other concentrations were prepared from this stock solution. INCUBATION: TTolarehtbseeitdttacetulossltthauwbarkaoeseusrtro,u2f3n05r0u0e0npmdclieelcrlawlsctee/omrsneftloiinfnrrucoeuomabucashatlnecigdoehnxintcpe(o1>nn0te5r0na0mttiipoalEnlfll/.yamtTg-2rbe-ossoewttktcio)numfglrtueoindrmoefsclfaulwuslukeormsreeoicnsnucoaletcnunurte^.,-t tubes of universal natural white type, at a temperature of 20 2C. Ttbhyheeftletuesostrtfelwascsaeksnsrcueena.cfhpoHrdaambyoefuaostru7gr2reomhwoetunhrtssm,waeanesrduesrpueebmrsfeaonmrtmsp.elAedslagosafli5ngdrmioclwawttehedrweoantaskdmeanteaafssruhormeeedt. 002414 L99a3b.1r2e-f4.no.: Page 6 of 31 DATA ANALYSIS: CALCULATIONS: Gverroswusthtirmatee,saws edreesccrailbceudlaitnedNbSy-EloNgIlSinOea1r0r2e5g3re.ssion on biomass (fluorescence) EfCso: Tce3of2hfn0eecmcietnngcht/olri.bnaicttieoonrntyroaefftifaoenpctrwoodanustcchtaeglcgivuriolnawtgethd50ob%yf SirnketedelrueptcotoinloaentmiooanfcgboresottwwatteuhemnrailtsOeOe(xEmpfCgr/e1sosas).enddTahsisthe NOEC: CfcaaoclnctcouerlnaAttriNoantOiooVnfANmOfeoaElnlCoww(iNethdotbOhyebBcsoeornnvtfreedorlrEomnffeiesacnpt.rCocoendcuernetrfaotriocno)mispadroinnegbeaycshingle TEST RESULTS: COMMENTS: VALIDATION: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance "FC-603 Water TM EAFTLCigTMht3%" EfCio - EAo EAo -170 100<EC90<320 NOEC: 100 GVVaarlaluupeekssPainarddeiIccnaaStlectauctloa3nt.e0cd.enbtyraintitoenrspogliavtiinogn.10N%O,E50C%araencda9lc0u%laitnehdibbyitiuosninogftghreocwothmrpauttee.rpUrongitrsaimn mmge/L Td(suhereeinrggersowuwlhttsihcahcruethrbveaegsser)d.owotnhgirsocwotnhsirdaetreesdfotrotbheeetximpoensepnatnia0l i-n7t2heh,ctohnetrpoelrcioudltures Tcv(3chea,o5lrelr-ydvrdbeaiecsylnhpidsmloioitntoryydoriicepnnrhtcgihtreetaenornaoisaal1ewggd,0iravbouseywnnintiathisnftahrdtaceuhttoerreairontneogfgsfmttehmpnoerroooterretemtsohttcahaaolanlnlynda0fr,1ot9eh6ufdeniun'7d1lf)2.ti,hlhtleehodteue,rcsisotnnpttetehrsratoiotlrdtephsHuelcdtsoidnwtnirtoohtl LSTRaeaemspbto.pprrleeterfd.rinaoetdocee:.::ived.: 0170-21.900091..301.219202..990-9490 tX ~ WoArtAnalyEEtlimcnaglHintoeercevhrenician SenQiorugaRelMietsyoeAalvrsecsrhusrSmacnyiecren"t"^is~t Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaulatbthoerapteorrmy aiscsciroendiintedwbriytinNgobrwyeRgFia-nMAiclcjdreladbit.ation (NA). The report may not be reproduced in any 002415 L9a9b3.1r2e-f4.no.: fage 7 oj 31 PRIMARY DATA: Growth inhibition test with Skeletonema costatum Test substance: FC-603 EF Light Water TM ATC TM 3% Lab. ref.: 99312-4 Start date: 07.12.99 Cone, unit: mg/1 Time: 07.12.99 08.12.99 09.12.99 10.12.99 Concentration 14:50 09:30 10:25 11:55 0 0.12 0.26 0.93 4 0 0.1 0.34 1.7 9.7 0 0.1 0.33 1.1 6 0 0.09 0.3 1.1 6.4 0 0.09 0.26 0.88 6 0 0.09 0.26 0.9 7 3.2 0.09 0.43 1.75 11 3.2 0.09 0.28 1.2 6.3 3.2 0.08 0.23 1 4.7 10 0.08 0.4 3.4 14.5 10 0.09 0.4 2 11.5 10 0.1 0.4 2.2 12 32 0.1 0.28 2.05 9.3 32 0.1 0.3 1.9 9.2 32 0.11 0.3 1.65 8.8 100 0.09 0.31 0.77 5.4 100 0.11 0.35 0.75 5.1 100 0.1 0.36 0.78 5.4 320 0.07 0.04 0.03 0.04 320 0.09 . 0.03 0.01 0.04 320 0.06 0.04 0.02 0.06 1000 1000 1000 0.09 0.07 0.08 0.05 0.09 0.03 0.03 0.05 0.1 0.03 0.03 0.02 Growth pH PH rate Corre- at start at end day-1 lation of test of test 1.22 0.998 1.59 1.000 1.42 0.998 1.48 0.998 1.46 0.995 1.51 0.994 8.2 8.2 "1.67 1.48 1.42 0.998 1.000 1.000 8.3 8.2 1.81 0.995 1.69 0.999 1.66 1.000 8.3 8.3 1.57 0.998 1.57 0.999 1.52 0.999 8.3 8.2 1.42 0.991 1.33 0.986 1.39 0.987 8.3 8.2 0.00 0.00 0.00 8.3 8.2 0.00 0.00 0.00 8.3 8.2 Reference substance (3,5-dichlorophenol): 1.5 mg/l 0.09 0.15 0.15 1.5 mg/1 0.08 0.1 0.12 1.5 mcj/l 0.08 0.14 0.18 Mean values: 0 3.2 10 32 100 320 1000 0.10 0.09 0.09 0.10 0.10 0.07 0.09 Reference subst. 0.08 0.3 0.3 0.4 0.3 0.3 0.0 0.0 0.13 1 1 3 2 1 0 0 0.2 0.2 0.22 0.2 7 7 13 9 5 0 0 0.2 0.28 0.35 0.32 0.908 0.968 0.921 1.45 1.52 1.72 1.56 1.38 0.00 0.00 0.32 0.998 0.999 0.999 0.999 0.988 0.969 The result of the reference substance (22% inhibition) is within the range normally found (20-80% ) 0 0 2 4 1 6 L99a3b1.r2e-f4.no.: Page 8 oj 31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: FC-603 EF Light W ater TM ATC TM 3% Lab. ref.: 99312-4 In biomass (fluorescence) In biomass (fluorescence) In biomass (fluorescence) 7 10 0 0 mg/l 5- 3 1 --11 0 -3 J 24 48 72 lusl U U I^U lN h o iirs ^ 7n I 51 S8 3 c -1 d 320 mg/l 24 48 72 test duration (hourfe 002417 In biomass (fluorescence) L9a93b.1r2e-f4.no.: Page 9 of31 Inhibition of the growth of Skeletonema costatum with: mg/1 ( FC-603 EF Light Water TM ATC TM 3%) Experimental data: Concentration in Growth rate FC-603 EF Light Water TM ATC TM 3% Control 1 Control 2 . Control 3 Control 4 Control 5 Control 6 Control mean : 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 320.00 320.00 320.00 1000.00 1000.00 1000.00 1.22 1.59 1.42 1.48 1.46 1.51 1.45 1.67 1.48 1.42 1.81 1.69 1.66 1.57 1.57 1.52 1.42 1.33 1.39 0.00 0.00 0.00 0.00 0.00 0.00 -- Inhibitic in percer . - 0 0.0 0.0 1.8 0.0 0.0 0.0 0.0 0.0 0.0 1.8 8.1 3.9 100.0 100.0 100.0 100.0 100.0 100.0 #02418 L99ab3.1r2e-f.4no.: Page 10 o f31 T est for Toxicity Marine herbivore: Acartiatonsa 002419 L99a3b1.r2e-f4.no.: Page 11 of31 TEST REPORT ACUTE TOXICITY TEST WITH Acartiam a r in e c o pepo d TtoHnE sd TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" i TEST RESULT: The 48 hours LC50 was calculated to 280 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ItoSOm/aDrIiSne1c4o6p6e9po-d1s99(C7:oWpeaptoedraq,uCarliutysta-cDeaet)e. rmination of acute lethal toxicity Test organism: Acartia tonsa University of C(Dopanenah),asguepnp. lied by the Marine B iological Laboratory, CULTIVATION: The test organisms were supplied as eggs, and were cultivated in accordance wanitdhatdhuelt"sPwareirseCuosmedminissthioente-sRting Test Protocol" (VKI). Large copepodites PREPARATIONS: Npasraibtourarstaiosl usfeosarew.thaetedri(lusatiloinnitwy:at3e4r.%To)hferowmataernwunaspofillltuetreeddstihteroiungBhyafjGorFd/Cwafisltuersed Test solutions were prepared from a stock solution. A stock solution of 1000 mwagt/e1rw. aOstphreerpcaornedcebnytrsattiirorninsgw1e.0re00p2regpraarmedsforofmthethpirsosdtoucctkinsol1ultiitorne.of dilution T2v5ihaemlstlewsoetfrwteeakssterpsuotnluunitnidoegnrlaaasns1d6c5ohn/a8tnahiimnliegarhlsst/,(d24a8rrkemppllihrcooatltloeepsdefrroiirmodeavacitahalsct)oenemacpcehnertcraoatuntirtoaenion. finTghe ~ 2d0ata s1heCet.. pH and oxygen measurements were performed as indicated on the ! 002420 L99a3b1.r2e-f4.no.: Page 12 of31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Acartia tonsa is expressed as the concentration of a product lethal to 50% of the test animals (LCs0). The combined mortality per test concentration were used for calculation. The 24 hours and 48 hours LCso values and the 95% confidence limit were calculated by using the standard procedure PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3, 1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. " TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-603 EF Light Water TMATCTM3 %'\ Acartia tonsa 24 hours 48 hours L C jo 280 (130-380) 89 (54 -120) LC 610 (490 - 790) 280 (220 - 360) LCso > 10 0 0 880 (620-1500) NOEC: 320 180 ctVhaaellcucuoelsmaitpnedudtibecyraptPerRo1gO0r%Ba,mIT5m0a%enaGalyrnsadips,h90aP%naddl9eInt5hS%atalcittoy3n.a0fif.dteern2c4e ianntedr4v8alhsoaurres.indUicnaittsedin. mNgO/LECVaarlueecsaalcreulated by VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved tohxeygpeenrcceonntacgeentmraotriotanliatyt tihnethenedcoonfttrhoelstewsat swlaesssgrtheaanter10th%an, aonrdeqthuea4l 8toh4oumrgs/1, LC50 of 3,5-dichlorophenol (reference) was in the range normally found. LSaabm.prleef.rnecoe.:ived.: TReesptoprterdiaotde:: 2909.30192.9-49 1007-.1021..1210.9090 Elin Horve ge Molversmyr ' AnalyEticnaglinteecehrnician SenQioruaRlietsyeAarscshurSacniceentist Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnifsusulle,dwbityhoaulat bthoeraptoerrmy aiscsciroendiintedwbriytinNgobrwy eRgFia-nMAiclcj0reladbit.ation (NA). The report may not be reproduced in any 002421 L9a9b31.r2e-f4.no.: Page 13 o f31 PRIMARY DATA: Acute toxicity test with Acartia tonsa Test substance: "FC-603 EF Light W ater TM ATCTM 3%" Lab. ref.: Start date: Measurement: 99312-4 10.11.99 Number of dead Concentration (mg/I) Control 32 56 100 180 320 560 1000 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 5 56 30 4 5 5 5 55 20 5 5 55 20 5 555 20 5 5 55 20 5 '5 6 5 20 4 6 54 21 6 5 55 20 5 NUMBER OF DEAD 24 hours A CE B DF Total 48 hours ACE BDF Total 0 00 001 0 00 0 000 1 0 00 0 00 0 00 0 0 00 001 0 00 1 0 00 1 0 00 0 0 11 1 2 13 3 4 54 3 1 11 11 2 12 01 0 24 32 5 33 95 5 55 16 5 4 6 8 16 20 Reference substance (3,5-dichlorophenol): 5 55 1 10 331 1.0 mg/I 30 645 001 3 0 11 9 IT The result with the reference substance is within the range normally found. S&T y. 002422 L9a9b3.1r2e-f.4no.: Page 14 o f31 Test substance: "FC-603 EF Light W ater TM ATC TM 3%" Lab. ref.: Start date: 99312-4 10.11.99 Measurements o f oxygen and pH. Start Concentration (mg/l) Oxygen (mg/I) pH 24 hours 4 hours Oxygen pH Oxygen (mg/I) (mg/I) Control 7.3 8.2 7.4 32 7.6 8.1 7.1 56 i n 8.1 7.1 100 7.8 8.2 7.1 180 7.7 8.2 6.8 320 7.7 8.2 6.8 560 7.6 8.2 6.5 1000 7.6 8.2 6.4 Reference substance (3,5-dichlorophenol): 1.0 mg/I 7.3 8.2 7.4 pH 8.2 8.2 8.2 8.2 8.2 8.2 8.1 8.1 8.2 002423 L99a3b.1r2e-f4.no.: Page 15 of31 T est for toxicity Marine Amphipode: Corophium volutator L99ab3.1r2e-f4.no.: Page 16 o f31 TEST REPORT ACUTE TOXICITY m arine am ph ipo SdEDCIMorEoNpThTiEuSTmWvIToHluTHtaEtor TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The lOd LC50 was calculated to >3060 mg kg'1 dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ATSoxTiMcityET13e6st7s-9w0it,hStManadrainrde aGnudidEestFuoarriCneonSdeudcimtinegnt1s.0 day Static Sediment TEST ORGANISM: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in afeeerdatiendg.seawater at 15C. A small amount ofnatural seaweed was used for PREPARATION: Natural seawater (salinity: 34 %o) from an unpolluted site in Byfjorden outside wSantaaasvlayunsseegdderffoowrraopsrrugeaspenadircifncogorntdetiesltnustteiaodnnidmwpearntoetpsr..orTStiheodenismseidletnimftoceroneltaleicschtaetdmesatu.tdadnyusannpdolaluntdedwsaiste Each test concentration was prepared by adding the product in 600 grams of damp sediment in a plastic bucket. 150 ml o f seawater was added and the bucket was shaken for 3 hours. The sediment was divided into three beakers, 400 ml of seawater was added to each, and the beakers were aerated over night. 10 animals were then randomly selected for each beaker. A sample of the damp sediment was analysed for water content, and all concentrations are calculated as mg kg*1 sediment dry weight. INCUBATION: The test was run in glass containers (600 ml) each containing 200 g of test sediment, app. 400 ml o f seawater and 10 animals, 3 replicates for each concentration. The beakers were kept under continuous aeration at a tinemdipceartaedtuorenothfe15datalshCe.etp.H and oxygen measurements were performed as 002425 L99a3b1.r2e-f4.no.: Page 17 of 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Corophium volutator is expressed as the concen mvtraaolturioteanlaiontydfpatehprerto9ed5s%ut cctocnloecntehfniadtlretanotci5oe0nl%iwmeoirtfeatrhueesectdaeslfctouarlnacitameldcaublslya(tuLioCsni5n.0g)T.thhTeehs1et0acndodmaayrbdLinCe5d0 PROBIT Analysis, National Swedish Environmental Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfcaoacnltccouernlAatrtNiaotOnioVonfAmNfeOoalnEloCwwi(etNhdotbhOyeBbcsooennrtfvreeordlromEnfeifsaenpc.troCcoendcuernetfroarticoonm) ipsadroinngeebaychsingle TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-603 EF Light WaterTMATCTM3%" Corophium volutator 10 days LCio 99 ( -760) LC >3060 LC*, >3060 NOEC: 3060 cVVaaalllcuuueelssaaitnerddeibccyaatltechue1l0ac%otemd5pb0uy%tePraRpnOrdoBg9Ir0Ta%manmlaetelhysGailsri,tayapanhfd?tea9rd51%I0nScdtoaanytfs3i.d.0eU.ncneitsinintemrvgalksga'1reseidndimiceantetdd.ryNwOeEigChta. re VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end of the test was greater than or equal to 60 %, the percentage mortality in the controls were less than 15%. The percentage mortality in a reference substance of 20.9 mg Fluoranthene kg'1dry weight fossoeebdrdsiieommrrvgeeeanndntt)iwacbascuisogtnn1tth6iefe.ni7cm%tabno.utrttTmathhloiteerytssaieinlldittityfmhrieaencnhtotiigonuhneseecisdsotnricescocefnonocmtuernnmadttrieoatnontdiob(e1nde0wwh6i0aigtmshhinegnro.ktagcWsic'g1eenpdihrtfeyaidcvwaelniemtig. ihtst Table 2. Characteristics of the sediment used for testing Organic content (%) 0.7 Silt fraction (%) 0.8 LTSRaaeembspt.opprrleteefrd.rioanetdcoe:e.::ived.: 99312-4 20.09.99 0-10.12.99 07:01.2000 Enclosed: Datasheets AnalyEEtliicnnaglHintoeecrehvrenician SenQiourgaRelieMtsyeoAalrvsceshursrSmacnyiecrentist 002426 L9a9b3.1r2e-f.4no.: Page 18 o f31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: Lab. ref.: Start date: Measurement: "FC-603 EF Light W ater TM ATC TM 3%" 99312-4 17.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 10 10 10 222 30 10 10 10 457 30 1060 10 10 10 30 3060 10 10 10 30 \ NUMBER OF DEAD 5 days 10 days ACE BDF Total ACE BDF Total 0 00 0 00 0 0 12 2 02 3 4 2 32 7 3 20 5 * Reference substance (fluoranthene): 20.9 10 10 10 30 122 I 5I 002427 L9a9b3.1r2e-f4.no.: Page 19 o f31 Test substance: Lab. ref.: Start date: "FC-603 EF Light Water TM ATC TM 3%" 99312-4 17.12.99 Measurements of oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 90 8J 222 96 8.1 92 8.1 457 97 8.1 93 8.1 1060 92 8.0 62 7.7 3060 100 8.1 93 8.0 10 days Oxygen (%) pH 97 8.1 94 8.0 97 8.1 96 8.1 96 8.1 Reference substance (fluoranthene): 20.9 95 8.1 92 8.1 83 8.0 002428 L99a3b.1r2e-f4.no.: Page 20 o f31 Data Sheets 002429 L99a3b1.r2e-f4.no.: Page 21 o f31 KMS-DATA3LAD (Kelse-, Miljo- og Sikkerhetsdatablad) Dokunentr.r.: Ersparter : 07--7297--0 Utgitt : 03/11/98 Versjon : 03.00 24/04/97 Skrevet ut : 11/11/98 Side 1 av ll HMS-databladet er utarbeidet av Miljeavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIFIKASJON AV KJEMIXALI3T OG ANSVARLIG FIRMA". Importer: 3M Norge A/S Kandelsr.avn: FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Deklaras jor.snummer, Produktregisteret: Telefon, Giftinformasjonssentralen: 22 59 13 00 i 2 OPPLYSNINGER OM KJEMISX SAMMENSETNING. Informasjon om enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VEKT-PROSENT VANN 7732-18-5 1,2-ETANDIOL 107-21-1 Faresymboler/risikosetninger: Xn; R: 22 2-(2-3UTOKSYETOKSY)-STANOL / 112-34-5 (DIETYLENGLYKOLMONOBUTYLETER) Faresymboler/risikosetninger: Xi? R: 36 SYNTSTISK OVSRFLATEAXTIVT STOFF Trade Secret FLUORALKYL OVERFLATEAKTIVE STOFFER . Trade Secret FLUORKJEMISX AKRYLAT Trade Secret TRIETANOLAMIN * 102-71-6 TYKNERE Trade Secret RESTER AV ORGANISES FLUORKJEMIKALIER Trade Secret 52 - 56 m 22.5 10 4-6 1-5 1- 3 a1 l- 3 Ikke kjent 002430 L9a9b3.1r2eJ-4.no.: Fage 22 oj 31 FC-S03EF LIGHT WATER (TM) ATC 3% AR-AFFF 3 VIXTIGSTS FAREMOMENTER. Vikcigste skadevirkninger Eelsefare: Kan vare farlig ved svelging. Kan irritare aynene. Sikkerhec: Miljofare: Spesialavfall. 4 F0RST IJELPSTILTAK. Tiitak ayne: Skyll straks aynene med rikelige mengder vann. Kontakt lege umiddelbart. Tiitak hud: Skyll straks huden med slpe og rikelige mengder vann. Fjern tilsalt tey. Evis symptomer oppstir, kor.takt lege. Vask tilsalt tay far det brukes igjen og kast tilsalt skotey. - i [ Tiitak innanding: Hvis symptomer oppstir, bring vedkommende bort fra ekspoheringskilden. Sarg for frisk luft. Hvis symptomene vedvarer, kontakt lege. i Tiitak svelging: Kontakt lege umiddelbart. Fremkall brekr.inger kun i samrid med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstlas person. : j 5 TILTAX VED BRANNSLUKNING. Egnet brannslukningsmiddel: Produktet er et brannslukkingsmiddel. 4? Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: 002431 L9a9b3.1r2e-f.4no.: Page 23 o f31 FC-603EF LIGHT WATER (TM) ATC 31 AR-AFFF Forbrenningsprodukcer ved brann: Prosedyrer ved slukkir.g av brann: 6 TILTAX VED UTILSIXTST UTSLIP?. Personlige vernetiltak: . Se vernetiltak under andre punkter i dette'HMS-databladet. Tiltak ved utilsiktet utslipo: Ventiler omridet. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale. Spill sanles opp umiddelbart. Rengjar omridet med vann. Plasseres i en egnet be'nolder med lokk. Fjerning av kjemikalieavfall: Se punkt 13, Fjerning av kjemikalieavfall. 7 KNDTSRING OG 0PP3SVARING. Betingelser for sikker' oppbevaring: Ma ikke lagres i omrider hvor produktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49 grader celsius. Lagres ved temperaturer over 0 grader Celsius. Hold beholderen tett iukkec nr produktet ikke er i bruk. Uforenelige materialer: Ventilasjon: Oppbevar emballasjen pk et godt ventilert sted. Sikkerhetstiltak for & forhindre brann: Sikkerhetstiltak for & forhindre eksplosjon: Ikkebrennbar. Sikkerhetstiltak for & forhindre statisk elektrisitet: Forholdsregler ved bruk: Hold emballasjen tett lukket. Royking forbudt: Under bruk av ii produktet kan reyking forirsake forurensning av tobakken og/eller L99a3b1.r2e-f4.no.: Page 24 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF 8 EKSPONERINGSXONTROLL OG PERSONLIG VERNEUTSTYR. 11! Oyevern: Unnga k fk damp, spray eller tike i aynene. Bruk tette vernebriller med ventiler. Hindvern: Bruk egnede vernehansker av hensiktsmessig materiale ved hindtering av produktet. Vernehansker av felgende inateriale(r) anbefales: Butylgummi. Annet hudvern enn hincvern: Unngi hudkontakt. ndedrettsvern: * Unr.gi inninding av luftbirec materiale. 3asert pl konsentrasjonen av luftforurensninger i arbeidsatmosfaren velges en av folgende type(r) godkjent indedrettsvern: halvmaske med filter mot organiske damper og vaskaformige partikier: A/P2SL. Svelging: Det m ikke spises, drikkes eller roykes under arbeid med produktet. Vask utsatte omricer grundig med sipe og vann. Vask hendene etter hinctering og far spising. Anbefalt ventilasjon: Hvis utsugningsventiiasjon ikke er tilstrekkelig, m& egnet godkjent indedrettsvern ber.yttes. Sarg for tilstrekkelig ventilasjon slik at dampkonsentrasjoner.e holdes under anbefalte administrative normer og/eller kontroller spray eller tike. Adm. normer for forurensning i arbeidsatmosfare, Februar 1996: VANN (7732-18-5) Anmerkninger'-: Inger, adm. norm. 1,2-ETANDIOL (107-21-1) 8-timers verdi ppn: - (stav) 8-timers verdi mg/m3: 10 (stav) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) 2-{2-BUTOKSYETOKSY)-ETANOL / (DIETYLENGLYKOLMONOBUTYLETER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). SYNTETISK OVERFLATEAXTIVT STOFF (Trade Secret) Anmerkninger: Ingen adm. norm. fT/>o ^ Tv VT /*,* L9a9b3.1r2e-f4.no.: Page 25 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF --FbGCRKJE'.lISK AKR,JLAg--(? r i.J z S e n e L)----------------------------------------------------------------- ---------- Anmerkninger: Ingen adm. norm. TRISTANOLA.MIN (102-71-6) 8-timers verdi ppn: 8-timers verdi mg/m3: 5 ! TYXNERS (Trade Secret) Anmerkninger: Ingen adm. norm. j j RESTER AV ORGANISKE FLUORXJEMIKALIER (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. ! 9 FYSISKE OG KJEMISX3 EGENSXAPER. Tiistandsform, farge, lukt: dH: ---------------------------------------------------- jIiI Gul/brun vsske. : 7.5 - 8.5 . Xokepunkt/-intervalI: Smeltepunkt/-intervall: < 150 C Ikke relevant !1 ! Flammepunkt: Ingen (Setaflash) Selvantennelighet: Ikke kjent | Sksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, ovre: Ikke relevant i Damptrykk: ca. 15.8 mmHg(Kalkulertv/2QC) ; Laselighet i vann-: Fullstendig Spesifikk vekt: ca. 1.06 (vann=*l) Damptetthet: ca. 0.88 (luft*l) (Kalkulert v/20C) i ___ j -" Flyktige organiske forbindelser (VOC): - 355 g/1 SCAQMD (Kalkulert v/20C). \ | Fordampningshastighet: Ikke kjent Viskositet: < 5000 centipoise 002434 L9a9b3.1r2e-f.4no.: Page 26 o f 31 FC-S03EF LIGHT WATER (TM) ATC 3% AR-AFFF 10 S7A3ILITST OG REAXTIVITST. Forhold som skal unr.gis : Materialer som skal unngis: Ikke kjent. Farlige spaltningsprocukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, gasser eller partikler. Dekomponering av bruksferdig blanding forventes ikke representere noen risiko. Stabilitt og reaktivitet: Stabili. Farlig polvmerisasjon vii ikke forekonune. 11 OPPLYSNINGER OM EZLSEFARE. Effekter ayne: Mild ayeirritasjon: symptomer kan vare redhet, hevelse, smerter og trer.' Effekter hud: Mild hudirritasjon -(etter langvarig eller gjentatt kontakt): symptomer kan vare radhet, hevelse og klae. Langvarig eller gjentatt eksponering kan fare til: Allergisk hudreaksjon: symptomer kan vare redhet, hevelse, blemmer og klae. Effekter innnding: En enkelt overeksoonering som overstiger anbefalte administrative normet, kan fare til: Pvirkning av sentralnervesystemet: symptomer kan vare hodepine, arhet, savnighet, manglende koordinsjonsevne, nedsatt reaksjonsevne, sleret tale, svimmelhet og i ekstreme tilfeller bevisstlashet. Irritasjon (avre luftveier): symptomer kan vare sirhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsak utfart med organiske fluorkjemikalier, som finnes i dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slavhet samt effekter som pvirker,neurologi; bukspytt, binyre og hematologi. Det foreliooer " V'*" L9a9b3.1r2e-f4.no.: Page 27 o/31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF veiledr.ing. Svelging kan fare til: Pivirkning av nyrene: symptoraer kan vare redusert urinmengde, bld i uriner, og rvggsmerter. Aspirasjonspneumoni (Lungebetennelse son skyldes at fremmedlegemer kommer ned i lungene. Sn alminnelig rsak til tilstanden er oppkast.): svnptoner kan vare hosting, pustevansker, gisping, Koste opp bld og lungebetennelse som kan vare livstruende. Informasjon om allergi, overfalsomhet: Xreftfremkallende eger.skaper: Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dezts produktet inneholder en eller fiere organiske fluorkjemikalier, soca muligens kan absorberes og lagres i kroppen over lengre tid, enten som utgangsmolekyiet eller som nedbrytningsstoffer (metabolitter), og kan akkumuleres ved gjentatte eksponeringer. Det foreligger ingen kjente helseeffekter for mennesker fra'eksponering overfor organiske fluorkjemikalier nr de brukes som forutsatt og i henhold til veiledning. Forekomsten av organiske fluorkjemikalier i blodet til den generelle befolkningen og underpopulasjoner som arbeidere, har yart pubiisert siden 1970 rene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. 12 OPPLYSNINGEA OM MILJ0FAAS. Persistens/Biologisk nedbrytbarhet: Dette stoffet er ikke lett biologisk nedbrytbart, men brytes delvis ned i tester for lett biologisk nedbrytbarhet. Dette produktet inneholder en eller fiere organiske fluorkjemikalier som muligens ikke er nedbrytbare og forblir i miljoet (persistente). Okotoksikologiske data: Dette stoffet er minimalt giftig overfor akvatiske organismer (100 m/1 < lave;*- rr;n rrco . n . . -rr-cn * L9a93b1.r2e-f4.no.: Page 28 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Andre skacevirkninger, rnilja: Data ora biologisk r.edbrytbarhet: COD (Chemical Oxygen Demand): 740000 mg/kg; BOD 5 dagers (Biochemical Oxygen Demand): 216000 mg/kg; BOD 10 dagers: 376000 mg/kg; BOD 20 dagers: 446000 mg/kg; BOD 28 dagers: 602000 mg/kg. Akvatisk giftighet: 96 timers EC50 for fisk (Fathead Minnow (Pimephales promelas)): > 1000 mg/1; 48 timers EC50 daphnia (Water Flea (Daphnia-magna)): 480 mg/1; 30 minutters EC50 Microtox: 124 mg/1;24 timers-EC50 Alge (Selenastrum capricornutum)): 170 mg/1. Behandling i renseanlegg: 3 timers SC50, Activated Sludge Respiration Inhibition:'> 1000 mg/1. Sarg for avfallshindtering iht. gjeldende fcrskrifter. 13 FJ2RNING AV KJEMIXALIEAVFALL. Metoder for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behandlingsanlegg for spesialavfall. Anbefalte avfallsgrupper: KOVEDKATEGORI 07: AVFALL FRA ORGANISES PROSES52R. UNDERKATEGORI 07 06: Avfall fra P3DB av fettstoffer, spe, rengjeringsmidler, desinfeksjonsmidler og kosmetikk. EAX-kode 07 06 03 : Halogenerte organiske lasemidler, vaskevasker og moderlut. Avfallstoffnummer: 7151 Organisk avfall med halogen. Spesielle komponenter: inneholder fluorforbindelser. 14 OPPLYSNINGER OM TRANSPORT. ADR-klasse: siffer: 002437 L99a3b1.r2e-f4.no Page 29 of31 FC-603EF LIGHT HATER (TM) ATC 3% AR-AFFF L9a9b3.1r2e-f4.no.: Page 30 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF reviderte merkeforskrifter av jar.uar 1998. Symbols Inger. Ir-neholder: 1,2-etandiol, 2-(2-butoksyetoksy)-etanol, Triacar.olamin Kan vare farlig ved svelging. Kan irritere eynene. Unngi innir.ding av danp/sprovtetike. Unngi kontakt med huden og eynene. Fir man scoffet i eynene; skyll straks grundig med store mengder vanr. og kontakt lege. Fir man Stoff pi huden, vaskes straks med store mengder sipe og var.n. Ved svelging, kontakt lege omgiende og vis der.ne beholderen eller etiketten. YL-gruppe: 00 Spesislle lover og bestemmelser: 1. Forskrift om utarbeidelse og distribusjon av Kelse-, Milje- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking m.v. av stoff som kan medfere miljefare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfall. 6'. Etc. 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR BRUKSRNSS SIKXSRHET OG HELSE. Anvendelsesomrider: Filmdannende skumkonsentrat for slukking av brann. Viktigste kilde(r) ved utarbeidelsen av HMS-databladet: Datablad fra- 3K General Offices, St. Paul, Minnesota, USA. rsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egne opplysninger: Informasjonen pi dette HMS-databladet er i henhold til vire nivrende opplysninger og beste oppfatning angiende riktig bruk og hindteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medforer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. 00243 L99a3b.1r2e-f4.no.: Page 31 o f31 FC-603EF LIGHT WATER (TM) ATC 3\ AR-AFFF F0LGENDS PRODUXTER DEXXSS AV DETTE HMS-DATABLADET : 3M ID-ttr : XF-0008-0223-1 3M ArFr/ATC FC-603EF Brannslukn ingsskum Produksjon 3M ID-nr : XF-0008-0209-0 3M Light Water3/3%AFFF/ATC Fire Fighting Foam, FC-603EF,1000 L 3M ID-nr : XF-0008-0208-2 3M Light Water3/3 %AFFF/ATC Fire Fighting Foam, FC-6Q3EF, 200 L 3M ID-nr : XF-0008-0207-4 3M Light Water3/3%AFFF/ATC Fire Fighcing Foam, FC-603EF, 20 L k 00l?440