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AR2a26. 3576 SMENVITOROMEENRTAOLLAHLBoOER0A8CT0OTR4Y2
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Conan yey
Analytical Report
Cay
Definitive Study: Quantitative Screening of Commercial Lots of Human Serum Obtained in 2004 for Endogenous Fluorochemicals Using Solid
Phase Extraction and Liquid Chromatography/Tandem Mass Spectrometry
Laboratory Request Number: E05-0442
Testing Laboratory
3M Enviro3nMmeEnntvailroHnemaelnthtaalnLdabSoarfaettoyryO.perations
-
-_
3M Environmental Laboratory
8 uz
Project Lead
Mark E. Ellefson
BuPiOldBinogx23-333E3-109 `Saint Paul, MN 55133-3331
Phone: 651-778-5405
"= 5
7 23
E4g 8% &
TeWsitliingamFaKc.iliRteyaMgaenna,gPehm.De.nt BPuiOldBinogx23333-3019
SainPthoPnauel:, 6M5N1-757581-3635-635331
Requestor BuiDladlinegB2a-c3o6n-09 Saint PaPuOl,BMoNx 3353513313-3331 Phone: 651-778-4736
Page 1of19
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WMENIRONMEENTRAL LONAOBOCR0A6TTO4R2Y
.
3M Environmental Laboratory Analytical Report
Project Number: E05-0442
1 Introduction / Summary
`The following analytical report presets data from commercially available normal
pooled human serum that were purchased by the 3M Environmental Laboratory and subsequently screened for endogenous fluorochemicals.
:
`Thirty-six separate lotsofpooled normal human serumwereextracted using a solid
phase extraction technique and screened for sixteen different fluorochemicals using.
liquid chromatography/tandem mass spectrometry (LC/MS/MS). Serum samples
were received from various suppliers between 9/9/04 and 12/2/04. Samples were
extracted on 5/11/2005 and analyzed 5/23/2005.
Heptafluorobutyric acid (PFBA) was included in the original listofsixtoen target analytes. However, due to instability in the methanol extract, analytical data for PinFcBluAdiwnigllthneotreb-eexitnzcalcutidoendofitn htheistrheiprotryt-.sixInlsottseaodf,acosmempearrcatiealstsuedryuwmiwlibteh conducted acetonitrile and analysis using a newly developed method that provides isomer resolution. Thoseresultswill be reported ina separate report.
Sample results are summarized in Tables 1 and 2. The average values reported represent samples in which there was detectable analyte. Sample values that were Less than the limit ofquantitation were not included in the average value calculations. Pease refer to Section for individual sample results.
Page20f19
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| Low
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Bis Spey |__NSotramnoderarofdSDaevmipalteiowinth ||05708[000622| 0.111|121 oss|ois|oars [ooua|
Table 2. Summary of Other Perfluorinated and Highly Fluorinated Analyte Results
[roe Tom[om | om [oncp [ges ge] [Wer T[[oowwwae"r[T[ooomrns |[oosowoweos[||aa2w5s8[[|ewsseas|wwwioi [||oaoowuws|||
Saratwaovwmaton |owwen|[oeoomn scoonms[[rooorm |[2amn||wwei|oowon|]
amSber iof artes wih RTA Te
NA? =NotApplicablebecausethisanalytewasnotdetectedinanyofthe samples.
wasn
--_-- worsamrs oonnerro:n
2 Reference Substances
wore
a
Name
a Synonym of | ce Material
Purity * SoFtaecettioory
[[oemteer[|rmnmimran||Gavwesmro ||rossormieon|nwoo|| ooomr]|
Ttommtroecsm||omonncriReenn|| wwnm ||Tomomeon||wmee|ooomn"|
Shs |eerie| won wm| won|
[[tFeobroteenmstons ||craore8s||omv0 mirma |oTreonmosmeron mean|wom]] [etme| rox| oi icmomon ase wen]
CoEToe| mew| war
Perfloroocanox acd,
Re i ammosan, oilopuicamlly|
i eT re
PROS[0 | RUtemasonsl
|
on
TCRIS6
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irs =
"NMR results indicate that the PFOS reference substance consistsof 30.8% branched isomer, and the PFOA reference substance consists of22.1% branched isomer. "Thesevalueswereusfe ortd he determinaotfitohne isomerratiosintheserum
samples (presented in Table 10).
nesters
-- fe--------------------------S--S--------------------
.;
ENVIRONMEPNTRALOLNJAOBEOEROASC0TO4TR2Y
3 Sample Preparation
"The samples for thisstudyware prepared as follows: 1. Theserum sampleswereallowed tothawand warm toroom temperature. 2. Ftoouar1m5ilmliLlitsecrrsoewf-sceaprpaemd(poorwlyaprtoepiyrnltehneeccaesnteroiffumgeetthuboed.blanks)were added 3. Mainspikes andmethod blanks werepreparedbyspikingtheserum (or wwaetreera)dwdietdhatshseuarnraolgyatteessoftionatlelrsesetr.umIsnaamdpdlietsio,nc,al'iCbraPtFioOnAstaanndda'r*d0s;,PmFatOrSix blanks, and method blanks at concentrations of 1.04 and 0.950 ng/mL serum respectively to evaluate extraction method performance. 4. Tenmillilitersofacetweoreanddied utotiheslereum andthecentrifuge tpruebceispiwteatree.capped and vortex mixed, allowing the serum proteins to 5. The tubes were centrifugedat approximately 3500 rpmfor20minutetso pellet the precipitate. 6. After removal fromthecentrifugtehe, supematant was transferred to abotle containing 70 mLofwater. 7. The sampleswere passed through apreconditioned SPE cartridgeand eluted with 2 mL ofmethanol.
Quaniitation was accomplished using high performance fiquid chromatography tandem mass spectrometry. The followingparameterswere used:
Lsyisqiueimd Chromatograph: Hewlett-Packard Series 1100 Liquid Chromatograph ACnoallyutimcatlemcpoelruamtnu:reK:e3y0stoCne BetasiITM Cys 2.1x100mm, Sum particle size Stop Time: 20.0 minutes Flow rate; 300 kL/min Injection volume: 5 pL. Mobile phase components: Solvent A: 2.0 mM ammonium acetate in ASTM Type I water Solvent B: HPLC Grade Methanol Solvent Gradient:
Tomme xm8w u[onR aw Bow am ax
Pages or19
--_--eee---- aw EvvRONEPNTRALOLCAsOTRAETiORsRYe
SMoafstswaSrpee:cAtnroamleytseTMtr:VeMrDsiXonS1c.i4exTM API 4000 Q-Trap Mass Spectrometer Detector Lonization Mode: Electrospray Negative TGeSmtp:e3ra5ture: 450 C EGn2tr:an4c5e Potential: -10 `Analysis Type: Multiple Reaction Monitoring (MRM) Table4. Acquistion Parameters
crmenima| commis [PEE|
(oem = eT =
PHA(CoAci)
PFOCAo(eGdAG)| mw || wwnmer_ me |
C(o=ho)d [tm |weams|awms n|
=
[rosa Tamre o Te]jo 5
ms
es
[p[ mP s r | 17o wwmes|
8.800 wocestus
5 2
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wwe
[[rPrroosAfi2TMoCs]1 | wsmoo | |wwwese
| owwe ||
qWuhanetnifmyuelatciphleantarlayntsei,titohnesrwebeyreprmoovniidtionrgedg,retahtetsoetnasliitiovnictuyrrtehnatn.
(TIC) was individual
used to Q3 jon
measurements.
4 Analysis
41 Calibration 413 Calibration Curve Alevneelxstarnacdteexdtcraalcitbirnagttihoenccuurrvveeiwnatshfeosrammeebmyasnpniekriansgtrhaebsbaitmspelreusm.aSttporcekscribed. somleutthainoonls.wSearmepilnedsivwiedruaellqyuapnrteipfaireeddvefrrsoums naea1t5f-upooirnotcehxetmriaceatlesdcaalnidbrdaitliuotnedcuirnve.
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SMENVIRONPMREONLTEACLLTANBO.OR0ASTO4RY2
`Theregressionanalysiswasperformedusing aquadraticcurvefitweighted 1x. Calibrationstaindardswerewithin+ 25% (+30%fortheLowerLimitof `Quantitation (LLORQ))with acoeffofidecteirmienantiton >0.990. Low-level calibrationstandardswhich werenotwithin 30%werenotincludedinthe cablliabnrkastwieornceunrovtei.nCcalluidberdaitniotnhsetcaanldiabrradtsitohnacturwveer,eenxoctegprteawtheertrheanno2texdt.hemethod 412 ContinuingCalibrationVerification (CCV) AContinuingCalibration Verification (CCV)wasrunatleast onceevery 10 samples,bracketingthesampleswiththecalibrationcurve. TheCCVrecovery waswithin 125%forallanalytes. 413 SystemSuitability Five systemsuitabilitystandardswereanalyzed beforethe initalcalibration curve: andaferth lastCCV.Thesesampleshadareacountswith a%RSDof<5%end faroeltleownitnigonitnismaen%ceRsS:IDof2%whenevaluatedindependentlyexcept forthe: "Thesystemsuitabilitysamples oreachoftheperfuorinatedacidsandFOSA failed. Ineachcasethe %RSDofthe retentiontimeswaswellwithintolerance: limits,buttheareacountsvariedbyasmuch as 20.3%.Thefilureofevery `perfluorinatedacidtestedsuggeststhepresenceofasystematiceffectontha class ofmalytes.Thisshouldbekept inmind whenevaluatingthedataprescaiedin thisreport. 414 LowerLimitofQuantita(LtLiOoQn) `TheLLOQforeachanalytewasequaltothenominalconcentration ofth lowest standardinthecalibrationcurvethatmetaccuracyrequirementsof70% -130% andthathadatleasttwiceas manyareacountsasthemethodblanks. 42 Blanks 421 MethodBlanks Methodblanksconsistedofaliquots ofMill-Qwaterextractedinthesame `maasnthensameplers. Allof themethod blanksinthisstudyhadareacounts <50%ofthe LLOQfortheir respectiveanalyteswiththefollowingexceptions:
PFHA:Twooutofthree methodblankswasgreaterthan 50%of the LLOQ;nonewereabove the LLOQ. C7Acid:Oneofthreemethodblankshadareacountsgreaterthan 50%of theLLOQ; nonewere abovetheLLOQ C11Acid:Oneofthreemethodblankshadavescountsgreaterthan 50% oftheLLOQ;none wereabovetheLLOQ
FOSA:Twooutofthreomethodblanksweregreaterthan 50%ofthe: LLOQ;nonewereabovethe LLOQ.
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ENVIRONMPERNOTUAELCTLNAOBOERSATOOvReY
PFBS:Twomethodblankshadareacociisgreaterthan50%ofthe LLOQ;oneshowedanomalouscontaminationandwasabove theLLOQ.
422 MatrixBlanks Matrix blanks consistedofaliqoufobltansk rabbit serum extracted i the same s`ampmlesa.astnMhaetnsiaxmepbllearsn.kscTohnrteaeinmiantgriexnbdloagneknsowuesrleopvreelspoafreandaalnydtoatnhaaltywzeedrweiatbhovteh.e the LLOQaredetailed below.
Table 5. Endogenous Analyte in Rabbit Serum
[sample |ToHA [GiiAcib|Pres |TWpros |THPFDS|
[Homme [owe | som | son |ome | ome |
[CMxBik 0505112| oocoszr | ooms | oor ower |oom|
I
[ Feaartsvoee5r |Ta Eooeodg sasdreT|h ow
ioeesri|ng
oocouleartsion|oF
Boemndeer
s|To
vo. o
|
"Theaverageendogenousvalueswereused ocorrectthematrixspikeresponse. 423 SolventBlanks
Methanol blanks were analyzed throughotuhetanalyticalrun. Analyte response waslessthan % theLLOQ inal cass.
Surrogate cPaFliObArat1i,on2s-tCan]daarndds,PmFaOtrSix['bl0a,n]kws,eraendadmdeetdhoasdsbulrarnokgsatteosctvoalalulatseerthuemesxatmrpalcetsi,on `bmuettthwoodopfetrfhoermPaFnOceS.[A0l3l]PrFeOcoAve[r1i,e2s-'weCr]esuorurtosgiadtee+r3e0c%ov,ereixetsenwdeirnegwtihtehsiunrr3o0ga%te,. recovery range to +/-34%. Specific surrogaterecovery data is presented below.
Page of 19
'.
SMENVIRONMPERNOTEACLLTANBOOERATOOREY
Table 6. Surrogate Recovery
PRFeOcAo[v1e,r2y:,7%]|| RPeFcOovSer[y0,3%
PRFeOcAo[v1e,r2y.,C%]|| RPeFcoOvSe[r?y,0%)
[room mosis T vow 1 vow TT wees | ome | aw |
[hosmcossvia | oax |oon || emsom |
[Tm
[uemwosostitT-- "oox
vax TT easws |
[oom ososiia| oom | wesw |1 weasesr [eee | vw |
[sosostsrT vuw 1 vox | 1 mearom|| vex TT mw |
[somstioa | owTeen | 1 iearoos | om |om |
[somesTawTow || measoss | ox | von |
[roosist| sw | es |] eae | vex |ow |
wsossrisa | vam HSOeriss | ow
|" ew | meas | ase | ew || mass | wee
| wes | | aw |
[wwomoToon Term TTTeaseTew | ee |
[oases TTT ew Tew TTT] eases ew | ew |
[ness TrenTT ren TT] ease: ew | een |
[m TTarees w s rawa TT eases ow 1ew|
[vases Te | em x || measws mw mew|
7NS TS
I"S
[mwas | emTem |Tivasre ww | mw |
[maoso | vow | em |T meas
vw | em |
[owneserTow | ew | 1 meas ww| ven |
[masons 1"7ew 17ew || measwse aw em|
[e |Ta een Ts eme | iene sox |em|
[wags | Tem
mew || measws ow ew|
[Covaer | ew] om |]Tease we| ew |
4.4 Laboratory Control Spikes (LCS) Six Laboratory Control Spikes (LCS) were preparedinrabbitserumand analyzed `with the samples. Results were corrected for endogenous levelsofanalyte in the matrix (where appropriate), and are presented below.
Pagesof19
---- i ---- ii
suBwROWEe NTA.r Asorks TonY
Tabl7e. Matrix SpikeRecoveries
Nominal | ovserved
IEEE A
| we
[[sE iossossveivisatz|| oomsee ||r oomse | |tdenem ||
Fhemostisa| aSsw | aams || wewm ||
Gr omer|e1 | omT 7 | mmT ] 1 Esowsse[om | om | mw |
bsososust| soa | sas | wow |
I-- bIsossrurse|I-- -- sos |Ia-- | em |--
Page 100119
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MENROMEPNTRAOLLVABEORNATCoOuERsY
Table 7. Matrix Spike Recoveries (continued)
[mCooomotsessa[|ooaann | |oomm | maxm]] [oF smosis oom Tr ome | am] FFesromsues[a--w--|----2s---- om] [mrrores--ome |rr ome Tr ww] [[eessmomsotsinssas|oossoo || oomw rowwow || FCeesmommsieissrTToammT|amou wrmm DisoserissTaoTso so | Fis r oamiinz e aan [osmostssToa | osm 1 [hwsoomsstsszT| oainT|asweT|owoxn]|
[[uissmumoossrnsisasTToommTooman auwx [Ciossoosess|| aa0mTooaens || omxw Fis ossTawTass Tv] [mrss[om [ows | mw [reryosostise | oso | ose | vmx] rex [hsoasiszTo2m0TTaT am0 | ooenw || vow C [hsousir osTTTom1 Tas 1| sow |
Pa11gate19
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ENVIRONMPENTRALOLANIBOOEER0A8TCO4RT4Y2
`Tablo 7. Matix Spike Recoveries (continued)
[JE
----
Fema fo [roe [ome [pe orm] wee |
[somstst| oa | oso | wee |
020
ozs |ow|
"ts osasiss|
Crsoss|
02200%
0225m1 [ oown||
[osososvsa | 20 | aes |wes|
Crsomsiss| a0 | as |ox |
Fr TT eee a ee)
lSoesitsr | oms |ows | om |
Ls 050 To | om me |s ew | 26%
[ry Chssoosmseusst|| oawtss | | ooaer | | owwx ||
[msomstsa | 15 | 288 | oom |
[wsossusa| ass | 200 | oox |
1
acosan2.002 [Cocosozmzoss| [Cocosopmaooe
oa
ore |sw|
oss | ozo | sow |
ace | sor | oom |
[e | em w |
Page 120119
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-------------------- SuEROMER NTALO LNAOBEOoRE sAoTOvRY
Table 7. Matrix Spike Recovaries (continued andcorrectedforendogenoustarget
analytes)
NCwoonrnc.i Loveentsoonaioano,m|| Ce"onCoemmreeror | rm
[Esme |oser |osm |oes | ow | eu| [isosotis0 032"|osu|oomsn| om roms|
[isomosv1ea|a0+0 |ane1|owno|rr 21 |oT e| [[siosomoostssto|[ooomr[|ooomm ||oooomm|| oomm || wumw||
premeemeee] [[rrssooswosstnisea|| 220071|22000 1 |oooomm = zomr ||on|
-- 11
TT--1
[1seons[oss |ose | woos osu |vou|
[ososostie |30 |ar | ocoms 206 | or |
--rosiat-- -- 1---- meres r Liwsswomsr oisuseyr|| aotye aomwe||oomwsse---- 5om0 ||-- ooww ||
[rwrorst-- | as--wow|=o-- om os--e =wow|
[ros mmi| szo as | oom am ome 1
Tsv[tlrrooobssemaio|
a5ce3olirs1 oe|D9Cocoownses
am
Tvl sdvA
1|xemow |n1 4
Fr
|
NcaRcuNiotnsR.eporedbecausemessublesousof geayeweena hsv.
Page 130110
SMENVIRONM"ENTPAL LRA0BOOR0A6T0CO0RY2
45 Accuracy The accuracy for each analyte reported was calculatedbytaking the average ofthe marx spike recoveries for that analyte. The results are presented in Table 8. Table 8. Method Accuracy for individual Analytes
[NFPA Tom | | FesA | sox | [Pra |ewTT7F|oS eam A|
[omaTwo | |" eras | sox | [pronTeams|Temas [sax |
[Conca| sox | [| Pros[zm | [CtoAdd|wm| |TRPFOS| sum |
CifAcd |sx | | ThPFDS | wow |
Clzhsd | wee |1 TT
4.6 Sample Related Comments "The extraction and analysisofthe samples wasperformed uiiizing a non-validaled rmeectohvoedri.esT,haendmatthreicxaslpiibrkaetrieosnulctusr,vPeFsOhoAul[d1,b2e-"c"oCn]siadnedrePdFOwhSe[n'*e0v,a]lusautrirnoggatthee accuracy and precisionofthe sample results.
5 Individual Sample Data Tables 9 and 10 summarize individual sample data. Table 9. Perfluorinated Acid Results
EEA50] Ra2 ons] oso swova|| sous |omen |over| [[[naCaesssm sio T[TrooeusssTT[ea<troioosna[|"o<owmrosea||Lossewsas||Looosrswes|||oooiwmsee| ||oooosmsetes||[oooomar||
[Aca [Tacs
|oses|oooess|oozsa|s72| wo|oso|owes | owes | | 104[oooess|ooses|aes| air|oss |oss|ooess|
TNA 604[4 ioLoumlom Lo Loan |om |om [oom |
TNAG36|0795|000 | 001064| 252 |10s |oma |orm |oon|
TNA 090[ose [oom |ozo [From LoseLowa] om
| zonfom]om |om se | sam
|ont | 10s
|oom | | oot
TThAAsGorSs||osesso|looooomessa||oosassa
||
asetoe
|ose|oor|os |ooo | uz|ow
|oouws | | eos|
Toe moe ofSk (ie50Be Wdmegee) 1
Page Hof19
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,
WEROMMEENTArL LAiBORAsTORY
Tabl8e. PeriuAockdrReisulnts(acotntianuedd)
| ny|0:02ocososloamod]one[oats |ose|ooume|acomr|
[[enemari[|oia2rTc0ui0oa|saemaess||o22s0[|oomae0|[socarre|| owoormn||<ciiiiogoa"||
[[rrkesot0Tv1o.705TTcoumiecso[[ooormsa[aor2o |0os0s0a||ooasmoo0|| oosseum||ooommae--]]
[[vriuseers[[v0sseTTc<utiiooaa |"oamwe|z2ms||staotr||oormeeo || ooeesm||ooooumers||
[r[Teuas7eose[[o1a0t0sT[c<uuiiooaa aatiemse||s3as z||armro|[oomms|| onvomze || ancoaizie|
[Tum|esestaso|E ozsy | ooeas |zEorl|T 100| au |r oz omer|
[rThnonooomsss||{a110n270T<[<usuooooao|"Coooooemuoee [|"osaoaonr[||owtrroets|| oooszaso ||| ooaeurasrs| oosuouiur|]|
[rnTTohrkoeosmoss[[|11v02s0|"T<c<uuuo0aa0l[|<a0iaw0m6|||a2a2ms0t[||ooooeemmm |||
oasant ome
||ootre[ooome|] |arr | som |
[[ruinseowss||"opzoesrs <<uuitooa0T|o=tueoa|| aarrr||oeosre||ooza ||ooomuss ||"owowoesr|e
|[ouTkumeesorrsa1|111300TT<<tuioono[|naoiws [|osa7s[aarsset|| nooamne |oovi;e <usieoisg_]
[rwese2orsr[cu100 ooo | 216 |wom|om our|<tioa|
[Tess ore <ui00[ous|1:2 oso| oz |ove | cua"
J [o on w "o2se1rT00am3o3s Tooeusrsse||osz7zs ||otoomr|| voeseres | |ooorrssz|| oeooms|]
|355%| onoom am|oz|oss om |ous | cove|
[oerArmGmTes4|fOkT | aoE|St ac|e Wa om|eo
|
as |
J.
ss |
0|
Page15019
--
wormssarsosroenne::
t[e[.Coolmmoneet[ uoverAdmoansaoR sue tLwooerseL| ag0o sPeiinzdeAonos |_Twaes |<ioo|ose|ocores|ser | 1s|<uoo|ose |
[mess Truce[om Lome |ows[an [eion |om | [mmm euoe | someloss |ss | 24[sume |om |
Psa tame eaefi oem |7wRes |<s uos o| 0133 | oowot | rs | as |<uoa |.oot |
[mem Luo[umeome] sm tes | ction[oo]
<woa| 02 |oooore | 286 | 124| <uoa |oes|
|_mnessd |<uoo| oses| oowr|sz | ws |<uoo|ooses |
[waeosr |ooosez |oss | ooze| so | wr |<uoa|ooss |
|p Jsuoa|oeLom [sot | wes Leuoo |owe |
TNAGOTS |<L|0 0120 7 | ooosss - sor |180|<ioo|ooss|
[aesrm |<uoa|ows | eos |oes |203|<uco| eos|
[mae
<iocalos2 <uoa| wiz | 12s |<uco |ous |
[mower Toon [osm Jono:ae[es suoo[oo |
ThA <uoo|oe | ean | 137 | 278 | woo |coms|
TNA6995 <uoq| oms |ooo| 288 | 11s | <uoa| coms |
TNA 6907 <uoa| oz | ome | 202 | 120 |<uco |ooars |
| Atom <uoa |oooos | oous| ser | 12s | <uco | oom|
| TNA7os <ioo oe {ooo| 343 |182|<uoo .oss |
TNA G33.
[nm [[wmAeessest TNA6055.
cue cumToma|om |ma riceToon| <100 |00067|ooze| 165 643|<u0o |oo |
<<ii.oo |ocioosrs||uooooosssesr|| 22sm ||12419s||<<uuooaa||oocoarrsss||
<LL0Q 0.0081 0.00688.
<uoa| oous
paLoi eLesg a Le o Lo {__waeses <woa| 0135 | poosea | 30 182 | <uoo| oozst | een | cuoo|oe |ocr az | 83 |<uco cows| [<ioa| 0124 +ooss? 280 | 1aa|<uoo| ocoto | [<uoa|000 |ooosr ood |221 | <too| oosss | [ae [<uoa|oss oor ser |tes |<tc|oome|
TNA |<100 |ooocy| ows| 1s |wz |<100| eset| Tnaeaot |<woo|oss <uog|ts |wa|<uoca | acme| [ness |<uoo|oomz ooser| wor|ws|<uco|eum|
.e
Ew ongaorny
Table 9. Sample Resultsfor Additional Perflucrinated and Highly Fluorinated Analytes (continued)
500eg [asso [ososs oso ars |13 [ote]oss [masses |<uoco own|<uoo| 14s | a7|<itoo|ooss|
[Mean Twa Tomo|ooteds| zs | tae| wa | cose |
[estan
na T0124 [ooo| ams |iss| wa | ows|
| wen [ooo [oats |oo |ate | zr| wa |oz|
r[eT wJorrmuwT +eT{wooen wcommw |iwernT|olotn| u0nT|oso]r|
wept weet 430
Table 10. IsRatoios mforIendidruatSampies
BFrcRaonOmceRhrYe..d ||| BFr"aeRnamcGehSres
wmeeeT e wa aT acioee [s uumn]]
I her---- ewTee-- s [-- sw-- e --a--|
wweess
weewses aemm aaen |
[m[ esamvwee nes Taam oome||
[ers weTesS
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6 Data/Sample Retention AElnlviorroingmineanltarlawLdaabtoaraatnodryt.heTahnealtyetsitcamlatreerpioarltshaanvdeabnealeyntaicraclhirveefedraetntcheest3aMndard reserve samples, as well as the samples perttaotihinspironjecgtarearchivedatthe 3M Environmental Laboratory. Rreetsaeirnveed sinamtphleeasr,cdhiigvietsaolfco3pMiesBovfiorroingmineanltadaltaLaabnodraatlloroyrifgoirnaapleprapieorddoatafawilelalsbte: 10 years following report signing.
7 Conclusion Uprnedseernttihne calolnsdiatmipolness.oftNhFePAst,udPyF,OeAn,doCgsenAocuids,fCliuoorAoccihde,miCcyaslAscwiedr,eFfOoSuAn,d tPoHbSe, PdoFtOecSteadndinTaHlPbFutDtShrweeerseadmeptleecst,edanidn tehveerCyy;loAtocfidswerausm.detTecDtHedAinan3d0PofB3S6 swaemrep.les. Cdeotnevcetresdeliyn,1PoFfH3A6 swaamsploensl.y dTeHtPecFtOedSinwa9osfn3ot6dseatmepclteeds,inanadnyFofBtShAewsaasmpolnelsy using the present methodology. PFOA [1,2-C] surrogate recoveries were all within 30% `fwohriilendPivFiOduSal[*a*n0a;l]ytseusrrroagnagteedrefcroovmer+i/e-sw1e%retoal+/l-w2it7h%inwh+/i-lo34ov%e.ralMletmheotdhoadccuracy uncertainty (based on isotopically labeled surrogate recovery) was +/- 34%.
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