Document 7GDdEdQD7DmQmrzb3qbOEmme

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:Mixturecontainingperfluorooctanesulfonawtheich may also be referredto as PFOS, FC-95, or as a component of FC-201 F. (1-Octanesulfonicacid,1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-p,otassium salt,CAS # 2795-39-3) Remarks: The 3M productionlotnumber was 1. The testsample is FC-201F(F-11129). CurrentinformatioinndicatetshatFC-201F isa mixtureof 3.0% PFOS, 36.0% diethyleneglycolbutylether,36.6% water, 8.25% acrylicfoamer,7.0% sodium octylsulfate6,.0% sulfonatedalkyl ethersodium salts,3.0% triethanolaminea,nd 0.15% tolytlriazole. The followingsummary applies to a mixture with incompletely characterized concentration of impurities.Data may not accurately retlecttoxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline203. Test type: Acute static GLP: No Year Completed: 1992 Species: Pimephales promelas Supplier:ETC, SuperiorW,I, maintainedatAScl-DETD, Duluth,MN Analyticalmonitoring:Dissolvedoxygen,Temperature,pH, conductivity Exposure period:96-hours Statisticamlethods: Not noted Test fishage: Juveniles(45days) Average Length and weight: 1 L Beakers: Length: Weight: Loading: 1.42 cm 0.034 g Aquarium: Length: Weight: 1.5 cm 0.041 g Beakers: 0.57g/L 0.34 g/L 0.17 g/L Pretreatment: Not noted. Aquaria: 0.21g/L 0.12 g/L 0.06 g/L Test conditions: Dilutionwater: Shallow wellwater,Two Harbors,MN. Dilutionwater chemistry: Hardness: 178 mg/L (as CaC03) Alkalinity: 147 mg/L (as CaC03) Lighting:Not noted Stock and testsolution preparation:Not noted. Concentrations dosing rate:Once Stabilityof the testchemical solution:Not noted. Exposure vessels: 1 L beakers having a surfacearea of 87 CM2 and aquarium having a surfacearea of 300 CM2 . Testingwas conducted at severaldifferenvtolumes in each vessel type to study the relationship between the surfacearea of the exposure chamber to the volume of the dilutiownater /testsolution. Number of replicates:two Number of fishper replicate:5. Number of concentrations: 1 plusa blankcontrolforeach vesseltype Water chemistry during the study: Conductivityrange (0-96hours): 322 - 391 pmhos/cm (controls) 359 - 400 pmhos/cm (1.0L exposure inBeakers) 361 - 373 pmhos/cm (3.4L exposure inAquariums) pH range (0-96hours): 8.22 - 8.63 (controls) 8.44 - 8.50 (1.0L exposure inBeakers) 8.37 - 8.51 (3.4L exposure inAquariums) Temperature range (0-96hours): 20.8 - 21 OC (alltestvessels) Dissolved oxygen range (0-96hours): 7.9 - 8.6 mg/L (controls) 8.3 - 8.4 mg/L (1.0L exposure inBeakers) 8.0 - 8.6 mg/L (3.4L exposure inAquariums) RESULTS Descriptionof testsystem Type of exposure chamber Surface Volume (L) Volume (pL) Surface area (CM2) of dilution water used oftest substance area/volume 3 h of dilution added water used 1-L Beaker 87 1-L Beaker 1 87 1 0 (control) 87 0 0.3 273 290 0 1-L Beaker 87 0.5 454 174 0 1-L Beaker 87 Aquarium 300 1 909 87 50 1 0 (control) 300 0 Aquarium 300 1 1 Aquarium 300 1.3 909 1,545 300 0 177 0 Aquarium 1 300 3.4 3,091 1 88 1 60 % Mortality 6 h 24 h 48 h 72 h 96 h 0 0 0 0 0 0 0 0 0 0 0 10 0 0 0 70 70 70 70 70 0 0 0 0 0 0 0 0 0 0 20 20 20 20 20 70 70 70 70 70 Element values based on nominal concentrations. Remarks:Testinwgas conducteodnthemixturaesdescribeidntheTest Substance Remarks fieldT.he valuereportedappliestothatmixtureand notthe fluorochemicaplroportioanlone. CONCLUSIONS No reliablceonclusionscan be made based on thisstudy.However, the data indicatetshatthe acutetoxicitoyfthetestsample may be dependent upon the ratiofthesurfacearea oftheexposure vesseltothevolume of the testsolutionT.he testsample appeared to be acutelytoxiconlywhen the surfacearea tovolume ratiowas about 87-88 cm2/L. Submifter:3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking2. Thisstudymeets thecriterifaorquality testing.However, the sample puritywas notpropedy characterizeadnd thestudylacksanalyticaclonfirmatioonftheamount of fluorochemical proportioninsolution. REFERENCES Thisstudywas conductedby AScl CorporationD,uluthEnvironmental TestingDivisionD,uluth,MN atthe requestofthe3M Company, Lab Request number Kl 792, 1992. OTHER Last changed: 6/26/00 ASCI Cmpmdion/ASel-DukA EnvkoomcnW TestinDgivision ASetReportID#015-FHA.R3M ASel StudyM# 5030-015-03 STUDY TITLE FURTHER INVESTIGATION ON THE ACUTE TOXICITY OF FC-201F TO FATHEAD MINNOW (PIMEPHALES PROMELAS) DATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED October 5, 1992 TESTING FACILITY ASCI Corporation AScI-Duluth Environmental Testing 112 East Second Street Duluth, MN 55805 Division Tel. No. (218) 722-4040 STUDY IDENTIFICATION NUMBERS ASCI Study ID# 5030-015-03 3M Company Lab Request# K1792 Notes: This report is a continuation of the ASCI Repoxt XD# 015-.FHA.R.3M for ASCI Study ID# 5030-015-0.3. The original study was completed on August 24, 1992. Sponsor.3M Con4my Sponsor study M# Kim ASCICorporatim/AScl-Duluth EovkoomenW TestingDivision ASclReporMt# 015-FHA.R3M ASCIStudyM# 5030-015-M 1.0GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representative Sponsor's Lab Requesti Testing Facility Principal Investigator Project Director Testing Facility Director Test Dates Data Validation and Report Review Further Investigation on the Acute Toxicity of FC-201F to Fathead Minnow (Pime ales promelas) OECD Guideline 203. 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106. Susan A. Beach,, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452. K1792. ASCI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55-805; Tel. No. (218) 722-4040. Jo A. Thompson Dinesh Vaishnav Donald Mount August Dinesh 28-September vaishnav 1, 1992. Report Signature: Retention of Raw Data and Final Report Location of Raw Data and Final Report Two years from the date study is completed. ASCI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, I-MN 55805; Tel. No. (218) 722-4040. Sponso3rM. ConVmy SP- Su* M# KlM 12 ASelCocporafion/AScl-Duhuh EnvkmneaW TediDSDWW= A&[ ft"n M# 015-FM.R3M ASelSb* M# 503"15-0 2.0OBJECTIVE(S)AND RESULTS To determine the effect of the surface area to volume ratio on the acute toxicity of the test substance, FC-201F, to fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the AScl study plan retained by the Sponsor, OECD Guideline 203 (OECD 1984), and the previous report submitted for this particular test substance. .lresults from this study are described in Table 1. 3.0 CONCLUSIONS 1. The test substance acute toxicity appears to be dependent upon the ratio of the surface area of the exposure chamber to the volume of the dilution water, but not on the type of the exposure chamber. 2. The test substance was acutely toxic only when the above ratio was about 87-88 cm-square I;'. 4.0 REFERENCE organization for Economic Cooperation and Development (OECD). 1981/1984. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Spcnwr. 3M Cmpany Spow" &udy M# K1792 13 ASclCocpmtionlASel-Duhith EnvkoomcnLdTedipgI)ivisim ASclReportIDI015-FHA.R3M ASCISU* ID#5030-015-03 Table 1. Acute toxicity test systems of 1,000 mg/L FC-201F (test substance) to fathead minnows (p. p Description Type of exposure chamber of test sy tem Surface area (cmsquare) Volume (L) of dilution water used 1-L Beaker 87 I-L Beaker 87 1-L Beaker 87 I-L Beaker 87 Aquarium 300 Aquarium 300 Aquarium 300 Aquarium 300 1 0.3 0.5 1 1 1 1.3 3.4 Volume (pl) of test substance added 0 (control) 273 454 909 0 (control) 909 1,545 3,091 Surface area/volume of dilution water Used 87 290 174 87 300 300 1 177 88 % Mortalit,r 3h 6h 24 h 4 0 0 0 0 0 0 0 0 0 0 0 0 50 70 70 7 0 0 0 0 0 0 0 0 0 20 20 2 60 70 70 7 /V Spmum: 3M Campaay Spauw SUWY IDI K1792 14 1