Document 77qmbMkXnzzNd2j0nMw0LywR

Wildlife International, Ltd. -1 - Project Number 434E-10I PFOS: AN ACTIVATED SLUDGE, RESPIRATION INHIBITION TEST ADDENDUM WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 454E-101 3M LAB REQUEST NO. U2723 U.S. Environmental Protection Agency Series 850 - Ecological Effects Test Guidelines OPPTS Number 850.1010 and OECD Guideline 202 This study was reported using a test substance purity of 90.49%. The test substance characterization was revised on 9/7/00 and the purity was determined to be 86.9%. The Sponsor requested that the results be revised based on the most recent purity analyses. The results previously presented were simply corrected by the ratio o f the two purities or a factor of 0.9603. No attempt was made to recalculate analytical standard concentrations or measured concentrations from original raw data. As such, minor errors due to rounding may be present. Attached is the revised summary page from the final report and the most recent certificate o f analysis. Wildlife International. Ltd. 2- - Project Number 454E-101 REVISED STUDY ABSTRACT BASED ON A PURITY OF 86.9% The effect of the test substance on activated sludge microorganisms was assessed by the Activated Sludge Respiration Inhibition Test Method (OECD Guideline 209). The test contained a control, reference and treatment groups. The control group was used to determine the background respiration rate o f the sludge and was not dosed with the test or reference substance. The reference group was dosed with 3,5-dichlorophenol, a known inhibitor o f respiration, at concentrations o f 3, 15 and 50 mg/L. The test substance was dosed at 0.87, 2.6, 8.7, 26, 87, 260 and 870 mg a.i./L. After an exposure period o f 3 hours, the respiration rates of the test solutions were measured using a dissolved oxygen meter. The respiration rates in the two controls were 39.8 and 41.1 mg Oj/L/hr, or a difference o f 3.2%. The difference in control respiration rates was within the 15% difference limit established for the test. The validity o f the test was further supported by the results from the 3,5-dichlorophenol reference group, which resulted in an EC50 of 9.0 mg/L. The EC50 was within the 5 to 30 mg/L range considered acceptable for the test. Differences in O2 respiration rates between the control group and test substance treatments were observed at all test substance concentrations. Following is a summary o f the results. Treatment Control 1 Control 2 3,5-dichlorophenol 3 mg/L 3,5-dichlorophenol 15 mg/L 3,5-dichlorophenol 50 mg/L Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS) 0.87 mg a.i./L Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS) 2.6 mg a.i./L Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS) 8.7 mg a.i./L Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS) 26 mg a.i./L Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS) 87 mg a.i./L Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS) 260 mg a.i./L Perfluorooctane Sulfonic Acid, Potassium Salt (PFOS) 870 mg a.i./L Respiration Rate Percent mg C^/L/hour Inhibition 39.6 NA 41.1 NA 31.3 14.6 22.9 63.8 5.1 38.9 87.4 3.6 35.1 13.0 33.5 17.0 37.9 6.1 32.7 28.1 3109..40 24.7 38.8 Wildlife International. Ltd.______ -3 - CERTIFICATE OF ANALYSIS Project Number 454E-10I INTERIM CERTIFICATE OFANALYSIS Test Purity1 N_Ca_me_n_et_r_e_A_n_al_y_ti_c_a3_lM_L_aRP_bR_reoofPerdevauruiterscoiinottrS:ycinpee:ePs#1c8F:(iCf96iOOc/S.7a9SDAt/%i0,o-L00n_R)1so_e8t_f2e_1r_e7_n_c_e_#_:__0_2_3_-_018A Appearance Identification " N White CrystTa, llinf e-5P1 ow: d' er ' ! " , j- * ' " ^ Result 86.9% Conforms TTT((MGLoooeCCttttaaaa/234657/1lllMMl.......s%%%SS(NMSPICMNI))IIIrCommmoiaoMaacdtPnlpppgknaciR/uuuunisgeMusrlrmreaimiiisnuStttiyyyemu)sm(e3NMR) ___ i -------- i TM- j pS / ...... ; ........... t i y ? it t ^ !* .* * * r <i ., j , " -.......................... ...., ,s...... -" ^ * * >i ?- ^ ~i ^ r ^ / i;*r" i * S i f f r rf ' . ty i??;- ..1i s 0: , .^ x ^ 1 111 ^ y J '> $ Positive 6245731.......1.9<000<613...0.0.00084w..0000403t1505990./11wwwwwwwtwttttt......%A//Att/vv.wV/vvvvwwttttt.....t%%%t%%..%% 8.41 wt./wt.% None Detected PROelAatAed Compounds - : * ,f ^*4* ~ JV V,, ' PRInueorsriidgt1ya.unbailyCcShDAolSnolvrCieoidnnetss((ITCG) A) is* 4* . >k,," > . > * * rf. ,> ! ' & * * 234567...... NNFBPSulhriiuttolorrfomsaairtpttieieehdd*eaete Orga4231n...icNTPHAFFFFdPAPBdAAAs *(IC) Elem23451e.....ntaCHNSFluhaiytAlirdrfobnuorroaogrinnlgeyenesnis*: 42351..... TTTTThhhhheeeeeooooorrrrreeeeetttttiiiiicccccaaaaalllll VVVVVaaaaallllluuuuueeeee ===== 06051%0%.79%.58%% N0.o3n3ewDte./twectt.%ed Not Applicable3 2463751....... <<<<<0080000.......5700000619040059706wwwwwwwttV.tAtttUVV..wA/vwwwwttv..tt%tt%t.....%%%%% 2431.... <<00..0012..8110 wwwwtttt....A/A/wwvvtttt...%%%% '34521..... 508114...2287..1444448wwwwwttt...ttAAA..AAvvrtttvv...%tt%%..%% COA023-018A Page 1of 3 Wildlife International, Ltd. -4- Project Number 454E-101 INTERIM CERTIFICATE OFANALYSIS Centre Analytical Laboratories COA Reference ft: 023-018A Date of Last Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen <-10C Re-assessment Date: 08/31/01 FPluuroirtiyde", 01.0509%%-+(NsuMmRoimf mpuertaitlieims,p1u.r9i3ti%es+, o1r.4g5an%ic+aLcCid/MimSpuimritpieusr,it0ie.s3,88%.4+1P%O+AInAo,rganic 0.33%) ToPtualriitmy p=ur1it0y0f%rom- 1a3l.l0t9e%sts==8163.9.0%9% 2Potassium is expected in this salt form and is therefore not considered an impurity. ^oPbuserirtvyebdyfDorStChisissgamenpelrea.lly not applicable to materials of low purity. No endotherm was 4idonSenoutertlhgfrumearniriinencasuattihnloteisno,snitanhmmethpeSeltehOeoa*ldepimpsceoenannordtstaiclttiooaonnnbasseil.dycesToirsneh,dveleeaarnnntdeiidoimnntgporuceSrosiOuntyfl*ti.daaegnnrdceehesetonwcteehlildsweitniettchetretphdreeutssauitnlifogunrt.heBased STHNPFFFFAPBPAAA HNTPereoinpfntltoauaffoflliuruuooooarrrcoooeppbtreiuocntpytaaarcnniicdooiaiccdaadcdidd sTheoretical value calculations based on the empirical formula, C*Fj7SOj'K+(MW=538) This work was conducted under EPA Good Laboratory Practice Standards (40 CFR160). i COA023-018A Page 2 of 3 Wildlife International. Ltd. _ -5- Project Number 454E-101 INTERIM CERTIFICATE OFANALYSIS Centre Analytical Laboratories COA Reference #: 023-018A LC/MS Purity Profile: ImTpCCCCou457t6railty Wt/1w.2t2. % 411...731324 8.41 Ncfaruvooretmvreea:tsgh,eTerhreCesesp4pCeoac4nntiadsvneeCdlfy6aC.cs6tTtoavrhnsaedlfuaCrroe5dsmvwctuaehlrrueveeeCscw.SaalLcasunickdlaaetClwec8duisluseats,taeitnndhdgeuatsCrhidn7egcCvuta4hrlvueaeenas.dwveCarsa6gcseatlarcneusdlpaaotredndsceuasfliiabncrgtaottrhisoen Prepared By: Reviewed B y :_D_JLSo__caah__ibve__noind__rFt__aiSls_t_ato._,_hBr_eCy_e_ret_Ml_yln__ta__rne_a_Ag_en_ra_,l_yC_tie_cn_atl_r_eL___aA__bn__oa__rl_a_yt__toicriaelsLaboratoriesDD__aa__tt__ee__! COA023-018A Page 3 of 3 ildlife International, Ltd. E C O T O X IC O L O G Y & A N A L Y T IC A L T E S T IN G SERVICES December 1,2000 Ms. Susan A. Beach 3M Corporation Environmental Laboratory 935 Bush Avenue St. Paul, Minnesota 55144 Dear Susan: Enclosed please find the Addendum for the following study: 1. PFOS: An A ctiv a ted Slu d g e , Respir a tio n I n h ibitio n T est W ildlife International, L td . Project No.: 454E-101 If you have any questions, please let me know. Sincerely, KRD/cyw8/beach Enclosures Edward C. Schaefer Manager, Biodegradation 8598 Commerce Drive Easton, Maryland 21601 -Tel.410-822-8600 - Fax 4 10-822-0632 *E-mail ecotox@wildlifeintemational.com European Office: Bergkampweg I*7231 CLWamsveld 'The Netherlands *Tel. 31(0)575 - 573848 Fax. 3 1(0) 575 - 574813 *E-mail:aleopold@svorldonline.nl