Document 71d42Rjq9KeZ20j28791ndxX6

ACUTE TOXICTTY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-3041. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 2300. The testsample isFC3041 (cc809-1).Currentinformatioinndicatesitisa mixtureof6% PFOS, 36% diethyleneglycolbutylether,36.05% water,12% Sultonefoanier,9% sodium octylsulfate0,.55% sodium laurylsulfatea,nd 0.4% tolyltriazole. The followingsummary appliesto a mixture with incompletely characterizecfconcentrations of impurities.Data may not accurately reflect the toxicityof the tluorochomical component of the testsample. METHOD: Method: Not given. Type: Acute static GLP: No Year completed: 1981 Species: Pimephalespromelas Supplier:ComniercialsupplierB,rady,NE. Analyticalmonitoring: Temperature,pH, and DO. Exposure period: 96-hours Statisticmaelthods: LC5ovaluescalculatebdy moving averageanglemethod. Test fishage: Juveniles Length and weight: Average length 41 mm Average weight 0.49g (wet) Loading: 0.61g/L Pretreatment: None Test conditions: Dilutionwater: Carbon-filterewdellwater,St.Paul,MN Dilutionwater chemistry: Hardness: 253 mg/L as CaC03 Alkalinity: 221 mg/L as CaC03 Lighting: Not given. Stock and testsolutionpreparation: The testmaterialwas added directltyo the dilutiownater on a weighvvolume basis. Concentrations dosing rate:Once. Stabilityof the testchemical solutions: Not noted. Exposure vessels: AJI-glastsanks(35x2Ox25 cm) containing16 L oftest solutions. Number of replicates:2 Number of fish per replicate:20. Number of concentrations: fiveplusa blank control Waterchemistryduringthestudy: pH range (0-96houm): 7.9- 8.1(controelxposure) 7.8- 8.1(320 mg/L exposure)* Temperature range (0-96houm): 18 - 19 *C Dissolved oxygen range (0-96houm): 5.0- 9.2mg/L (controelxposure) 0.2 - 9.2 nig/L(320 mg/L exposure)* 320 nig/L(second highestconcentrabon)exposure values used because the highestconcentrationused resultedintotalmortalityby 48 hours. RESULTS Nominal concentrations: Blank control,87, 135, 210, 320, and 490 mg/L Element values: 96-hour LCw = 240 (217 - 265) mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on a mixtureas described inthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hour LC50 forfathead minnow was determined to be 240 mg/L witha 95% confidence intervalof217 to 265 mg/L. Submitter: 3M Company, Environmental Laboratory,P.O. Box 33331, St.Paul, Minnesota, 55133 DATA QUALITY Reliability:Klimischranking2. Testing meets the criterifaorqualitytesting. However, sample puritywas not propedy characterizedand the studylacks analyticaclonfirmationofthe amount of fluorochemicalproportioninthe solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory,St. Paul,MN, Lab Request number 6508S, 1981. OTHER Last changed: 6/26/00 LL'4VIKUNMLN'I'Al, AQUATIC TOXICITY DEFINITIVE (FU LABORATORY DATA SHEET Type Te Material Tes Test Crganism e Da te Rec Id__ZZ -,C-A/l ELatch #A- fl@ Avg.Wt.A AIC@grams Avg. Size oV, / an bb. organismms per dose-,,,@o Toar3i7ng Factor Fish Disease Treatnent: g/l Test Solution Volume/,& lAters/All-Glass 1'6ark Tank Dimemicns: 35x2O an and 25 an deep Time Startecl_,Y,', Date Started x @EIA Dilution Whter-Carbm-filtered well water (Twp. Date Ompleted-z,// C. , D.0. ppm, @5/ ql Irmestigator-,O"/,P-pa7;5P'-,-S (Full Signata@W-,/C@-/ 106 LLsr-D vi" Tw* Initial Total 24 HouLi No. Conc. Amount Tbtal No. mg/l Added Dead Oontroll (95%01@@ Limits) c6r4a. LCso ZAIOLKW a&uw.AN6c1men= CB Hotirs- 72 Hours D.0. Pbtal No. pH D'0.. 7btal th. Dead RM Dad D.O. ippn 96 Hours 7btal rb. pH D.0i, Dead IPPM L-A Seoondiary Effects !A (other thm Noruuty): i TarLk ND. Symptcms or Reactions pontrol, 24-Hr. 7bta ILNiUimber Attectea 48-ar. 72-Hr. AL L-, -AL L -AL LL A A 96-Hr. - A., V L Li j. tti, Ljt%Mkj Z%J-% I UA I AQUATIC TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) 7ype Test-!?@@ e\l-')7jr,#'MteriTaelsted Test Crganism kmFAQ Avg.Wt.&_4 grann Avg. Size 041,z ea@@Date RecldlXV &_Batch cinNo- Organi_qW per dose_Zo loading Factor Fish Disease Treatna t: Test Solution volume /,,(a IAters/All-Glass Tark Tmm Started--A,'& ajW idatest,:@ Tank DiMemims: 35x2O cm and 25 an deep Date Completed_4/ Dilution Water-Carbon-filteredwell water (Itup. OC. p D. 0. pH_d,/ irmestigator (Fulls Tarik Initial Thtal No. Cow. Anotmt mg/l Added OVIIJ[AA 24 Hburs Thtal No. Dead 0--r Cntr. aWo5 (95% t tits) "f /- K(3. L@,so 7.,%@o@o 48 Hours @o.r@tal@l No. pH 72 Hours 96 Hours D.O.ITotal No. pHID.-O.7btal No. ]pH D.O. pM I Dead ppn Dead IPPM *QC, A2 n tmm, ac - tiais & Diite- "I@rY9LPEffefctsfect(so(othe@r thettrhan-t Mwt&lity): Tank ND. SYnPtCMB or Peactions 24-W. Dontrol ic --AlA A a PA i- - Ofected 48-Mr. 72-Hr. 4A zo iFF, PMMV k 96-Hr.