Document 70pLLjdznyx15Kmo15MZLeL66
AR 226 -- OBGH
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity: ArefmeirxrteudretocaonstaPiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuoimfponoanteen,twhoficFhC-m6a0y3Fa.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3).
6Rd0iee3mtFah.rylkCesun:rergelnyTtchoilenfb3ourtMmylapteritoohdneuric,ntdi8io2cn.at9leo5ts%niutwiamstbeaerr,miw2xat%usrae5cor0y1fl.i0c.Tf5ho%eamPteeFrsO,tSs4,a%m7pA%llkeylisetFhCe-r sChuyhldifroaortxeo,iadl1el,.y4l0)5.h%0eXx3aa%mnictinhsia-un1m-gc(h3ui-moC,rhild1oe.r,o4a0%l.lc5ya%ll)ct-ir3ui,me5t,ah7ca-enttoralitaaemz,ian0-e1.,-0a07z.%o0nNi5-a%(a3ds-aomdaintuamne chloride, and 0.05% tolytriazole. cThhaerafcotlelrowiiznegd scounmcmeantrryataipopnlsioefsitmopuarmiitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical componentofthe test sample. METHOD: MTyepteh:odA: cOeECstDatGicuideline 201, and EPA CFR 40 797.1050 GYeLaPr:sNtuody performed: 1995 SSpoeucricees:: ISne-lheounsaestcruultmurceasp.rOriicgoimnuatlluymfrom ATCC, Rockville, MD EElxepmoesnutrebapseirsi:odA:lg9a6l cheolulrcsounts (cells/mL) and growth rate. SAtnaatliysttiiccaall Mmoentihtoodrsi:ng:NoNtoatpgpilivceanble due to study results. Test Conditions: AmlegthaoldN.utrTihiesntnuMterdieinutmm:edNiuutrmiesnetrvmeeddiasumthfeordiflrueesnhtwfaotrearllalaglgaaelpoeprerOatEiConDs. The pH of this synthetic algal medium was 7.9. wSatoscpkraepnadrteedsbtysodliluuttiiongn 5p0r0epmargatoifotne:st Asu1b0s0t0anmcge/Li.n p5r0i0mamriy. sotfosctkansdolaurtdion algal nutrient medium. Aliquots were then added directly to dilution water in ~~ tEexspt ovsesusreelsvetsoscerlesa:teNotetsgt isvoelnutions. ANguimtabteirono:f Nroeptlgiicvaetens: Not given.
Initial cell loading: 1x 10 cells/mL.
002453
Number of concentrations: one plus a blank control WLiagthetrinCgh:emCiosnttriynu:ouNso.t given RESULTS
Nominal concentrations: Blank control and 1000 mg/L.
96-Hour Algal Growth Response
: Cool
1000
Mean Growth Rate
Percent Change in Growth Rate
|[ado s | |s . -- sae||
Element values: 96-hour ICso = >1000 mg/L
Element values are based on nominal concentrations.
Reversibility of Growth Inhibition: Not given.
Remarks: Substance
TReestmianrgkwsafsielcdo.nTduhcetveadlouensarmeipxotrutreed
as described applyto that
in the Test mixture and
not
the fluorochemical proportion alone.
CONCLUSIONS
_
--
FC-B03F exhibits a 96-hour Iso of >1000 mg/L. This concentration inhibited the algal growth rate by 34%.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
R`eSlaimapblileitpyu:ritKyiwmaisscnhotrapnrkoipnegrl=y 4c.harNaocttaelrlilzaebdoraantdortyhedasttaudcyoulladckbsealnoaclaytteidc.al confirmationofthe amount of fluorochemical proportion in the solution.
REFERENCES
TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbnyutmhbee3rMM1C6o5m5p,an1y9,95E.nvironmental Laboratory, ~~
OTHER
002454
Last changed: 6/26/00.
002455
BEST COPY AVAILABLE
ENVIRONMENTAL LABORATORY
FRESHWPARTEEPRARAALTGIAOGNRWOORWKTSHHIENEHITBFIOTIRON TESTTM"
tPnasceeeseotFow
irs DATEIOA AS.
izeFea2sse loa= nigd) Fufeurti.ve,Cormiaall,OUukhiesseeoantllaing soLtlitudeor,
we
2
Tiara AlgalNutreat Ueda 0a qrepaced;
5
BT Bakao m na e e ree TTo ooe e A e C eene o Ge P tk h ey
133)
Biit sm T
298 Svausfor aliey lubiial, Y 3
CS tOeNah Jos IE = a FI2 TOSTECREX
Po
Te TAGS
[0 A TA PREY TRET
BAsieeseC e Asoe o "lon nReO AE BTEYEUIDE[E R1 D0 TA eA T--
a FEChtoCount)K108lLfull A
8557Tost mehodolog conwfiot thre molsowing tw tt proocolsTLFS RETS
z
wien
(R35R5OEoCD120a1oe"ga, GrowthiIonhbioSnaTess"Si3ndFed.SE RGT ReqT Vol 40,R No:S O7S57l.g 1Ac0i5eT0on;epTest ANS
felo on nan. p CLRnCOR Se a eR ol BT
os
>
BRE a de
enc
ey ER: 002456
BEST COPY AVAILABLE
NUTRIENT
`STANDARDENNUVTIRRIEONNTMMEENDTIAULMLFAOBROFRRAETSOHRWYATER ALGAE CINOSNTCOECNKTRSAOLTUITOINON FINIANLTCEOSNTCSEONLTURTAITOINON
STOCK SOLUTION 1: MAGRONUTRIENTS
MoooC]h 640
250ot
1152 mmoort
CaCl, 240
18 ot
1 mot
MKgPSOO,, THO
a15t60ott
1T5ommoolt
STOCK SOLUTION 2: Fe EDTA
nLaatemses rM3a1s9t4
FeCl, 6H,0 Na,EOTA 24,0
80 mol. 100 mot.
STOCK SOLUTION 3: TRACE ELEMENTS
M#n8C0l, 440
a1855 mmoorn
2CCnCuolCCyl 624400 NaMoO, 240
013051 mmmooottt 7 mot
80 vol. 100 ug.
awss wunt
3o105tuwwgott i
7 wt
:
STOCK SOLUTION 4: NaHCO3
NEE
NatCO,
EP
50 mp
Striz tocksoktions 1-4bymembrane faton 0.2m).
. si
Bo SOLUTIa ONS!NUe TRIENTCONCRENTERATE EIRZLA: ~~i | B iL e
bCe o aoa otmaoissstoTotcockscsokousummooownnmsz:o:n|1 4 1ESUIRBGEREEEARS L BL E EEHR
a G = a Elnl
hc LL CaS nd 1S 10mofL STOCO K SOLR UTIE ON1.A3 L 3.1 + evCsR Ha
simu pb
;
fo
Fe eee
big rtm
TR
1viaT d BRAGGR1G00mot the pe; aduste HeensyCCoonnc obin 109S0o0 MUCH
Se MEO: ial pH medium lier squiBralon willSCe.Of
1
apariGREE a SRR 2 SGRE
a 002457 F
z C[L | [EL e] aes _
moNENTAL LasoRATORY
oman
ji
uy
FRPEAeLS GnAGcH RrOWoTW wHIRNHAA ITBEIRTIT ONT7EESTAR
cnses rot i,smn
A
2g
-
a
ok BTATES:Beles OVETEATS1At
I} Ee fusesEaemors buses asour:
Ce
En me )
e4d
Il tnamotan
2
!
T
5
3 scxocomuna,,
20
PE H SoEr | --
lEddE
=
fo [or[oe = =[ | EEJ 3
RE 3:
=
=[= |= [1H )
:
re
EE
ey TwE e sTaUeS)ER
ee
-