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- ADIETARYLCS0STUDYWIrToHTsHENORTHERN BOBWHITE WILDLIFE INMTELRANABTRIEOQNUAELSLTTDN,o.PRO0J2E7C23T NO 454.103 S5r U0S.-BEanGlvc ioPrgoSnmeNnatiamlciPesr5T0eo2tc2G0Ad0gnecsy (a) FIERA SubdiviSesctiioon n71.,2 O5CDGitlin 205 AUTHORS: SCScooamnu.BrGeyaleClaessery Remand Van oven STUDY INITIATION:Apri 21,1599 STUDYCOMPLETION: Api26,200 AMENDED REPORT DATE: March 25, 2004 EmS3iUMBCMnomIoTrLsTaibTEoonDOr Ppaiimteysoenn5184 Wildlife International, Ltd. o[nr,rMy-- a 2160 0 zas00 Page 1016) amen 600594 - WILDLIFE INTERNATIONAL, tT ES PROJECT NO: 454-103 GOOD LABORATORYPRACTICECOMPLIANCE STATEMENT SPONSOR: 3M Corporation TITLE: BFOS: A Dietary LCS0 Study wtiheNtorthhern Bobwhite WILDLIFE INTERNATIONAL LTD, PROJECT NO: 454-103 MLAB REQUEST NO: U2723 STUDY COMPLETION: Apel 26,2000 AMENDED REPORT DATE: March23, 2004 h"eThUiSs.stEndviwraosnmceonntdaulctPerodtienctcioomnplAigaennccey,wit4h0GCoFoRd LPaabrotra1t6o0ryaPnrdac7t9i2c,e S1a7ndAaurgdussatsp1u9b8l3,isOheEdCbDy P1r9i9n2ci)plsensdofJaGpoaondMLAaFboFr,at5o9ryNPorahcatinc,e,N(oOtiCfDiEcaItGiDonI(N2o). 3328,5E0,nvAigrronimuelnutraMlonPorgodruacpthioNno.Bu4c5a,sP,ris1,0 August 1984 with the Following exception: `GLAPttchoemptliimantceh;etheoswtewvaesr,cotnhdecuhcatresdctehretzaettiosnubwsetasncpeerwfaosrmneodt ccohrdairnga0cit3neMacrSctoiarnzddaaenrcddeOpweirtahtfilnlg PSrocuedubrewssaatnrdeasMheatnrhaocdctse,ieeadndinasllccroondwandcatewairteh GbLeiPngstmanadianradisnfeodllionwitnhgest3udMy carocmhipvteos.n.The test wa"sTnhoetstdaebtielirtmyinoefdthien taectcsrudbasntcaencweiathnGdoroefdeLraenbcoerasttoanrdyaPrrdaucntidceerSctoannddiaridosn.sofstorage a the est sit: accAonradlaynsceeswiotfh GliLvPersatndasneradanasdarrmepsdluelsstscofotnhdeuscteeadnablyyse3ssM.eCboeripnogrraetpioorntewdesreeparnatoelyp.erformed in STUDY DIRECTOR: , SenN. aGaalla}gh.er 2 den Senior Biologist pars 33oy SPONSOR'SREPRESENTATIVE KMhEmaTrAnnBeaach. [Deach ote 3/24 Joy _ AMENDED 20595 WILDLIFE INTERNATIONAL, L0 a PROJECTNO. 454-103 `QUALITY ASSURANCE STATEMENT `This study was examined for compliance with Good Laboratory Practice Standards as published PbryintchieplUe.sSo.fEGnoviordonLmaebnotraaltoPrryotPreaccttiiocne,Ag(eOnCcDy,E/4G0DC(F9R2)P3a2r,tE1n6v0iraonndme7n9t2,Mo1n7oAgurgaupsht N1o9.894;5,OaECsD,. 1992); and Japan MAFF, 59 NoSan, Notation No. 3850, Agricul Production Bureau, 10 August 1984. The dateofal audits and inspections nhed dacs tht any findingswerreportedto the Study Director and Laboratory Managemwernts follows: Activity "&TetAnSaulbysttiacnaceSaPmrpelping Matix Fortification F&eeAdnCaolyntsiucmaptSiaomnpling AsDsrayftticRaelpoDrtaa and BiroalotgyReDpaoratsnd Final Report ARmeepnodretd Final DATECONDUCTED April 2, 1999 April 2, 1999 April 27, 1999 Jy 7,8,9,1999 AUEISt26,27,30,31, 1999. Api 7:18, 2000 March 19,2004 STUDYDDAIRTEECRTEORP.ORMTAEDNATOG:EMENT Aprl2,1999 Apilz2, 1999 Apel, 1999 Apil23, 1999 AIZLI99 May 4,199 1iy9,1999 Jy 16,1999 Augusta], 1999 September 13, 1999 Apel 18,2000 April 19,2000 Mah19.2004 Mach 19,2004 SanS.uCsolamann. Colman Senior Quality Assurance Representative DATE___3-23.04 AMENDED Gn0596 WILDLIFE INTERNATIONAL, LTD . AMENDED REPORT APPROVAL SPONSOR: 3M Corporion TITLE: PROS: ADietaryLCSO Study with th Noster Bobwhite WILDLIFE INTERNATIONAL LTD. PROJECTNO 454-103 3M LAB REQUEST NO U2723 , STUDYDIRECTOR: Ndr $ Blacher Sean P. Gallagher `Senior Biologist, Avian Toxicology CHEMISTRY PRINCIPALINVESTIGATOR: 2 Lan cIveNn, PHhY.D Scien, Analytical Chemistry REPORTAPPROVEDBY: Cintta KtMsitcZhellNacht! ManagerofEcotox Operations. WilHdB.iNilxodn,.Fo = Dietor of Chemisty Salo Date Biesass Be23Hank oo) Bie yw : ----. AMENDED 600597 -_ WILDLIFE INTERNATIONAL, LTD 5% PROJECTNO.: 454-103 `TOAF CBONLTENETS TIEPM srw E GOOD LABORATORY PRACTICE COMPLIANCE STATEMENT... POE 2 QUALITY ASSURANCESTATEMENT... rn PoE 3 REPORTAPPROVAL vss:FBS 4 TAOFB CONTLENTSE... PUBS 5 THAGI mms ----------age SUMMARY. s--" - nnn BOGE1 re Page OBJECTIVE. - nvm POE 8 MATDEWERA ssessssmmmmesimmm cage J SOAS -- s-- . renPaPaggee 89 DLuEraoTtftiheToemns..m. m" r e-- -- Crepeagse f AEE De bhcnSm--mnmmasmrmemnmmmmm------------------------------------g 1 UALISTBERNIoG mmmnmrscommeamp--r--m--a--m--m--m--m--m--s--m--s------------------tT0Y}1} A -- > >.1 Co OSTA t memmammmmmmmms--m--e--m--m--o--m--a--m--a--m------I 718 SHEA A wnmsermmsrmmmmmmm-------------------- RESETS mmmmmmmmmommmsnsmmm---------- EAS a `MoandrCInitcalOaBSErlvaiOsns --------r----r----r------------------ PGE 1S BGord08y8WNeCiIgOhPtSYa.ndFeedConsumpion....... - m--me-- so g Page 17 CONCLUSION.......... wn wo on POE 18 REFERENCES ..... win S------ 1 610598 ------------------------------------eeerereereeees WILDLIFE INTERNATIONAL, LTD . PROJECTNO.: 454-103 TAcOoFBnCnOeNLTdENETS TABLES AND APPENDICES TABLET; CSumyisivePMROrS i rom NoreBobwhiteAce icyToxic P20 TABLE2: TABLES: TABLE 4 S Mean WBE EodRyPRWeY OiSght(g) rere fr aNo orthm em BobwhiteAcuteDietary Pgs Toxicity 21 NMeeaneFDeieadrCyonTsoumxpitinonS(obyiwridthy)PROfSro.m Northen Bobi Page GRrCoupDGiroasrsyPaTthoolrogyicaSl OybsewrivhatPioOnSs.romar Northeo r Bobwhc ite POGS2S TABLES: Cumulative Mortality (Estimated Cumulative Dose,mg/kg)from a Northem Bobwhite Acute Dietary Toxicity Studywith PFOS.................. Page 26 APPENDIX; CoifofcAtTeS ccc Page APPENDIIXE DekFomineFOG 28 APPENDIVX: DekPepin... Fog43 APPENDIIIXI: Analytical Methods andRESuMS...........cnvsvsmsmrsmmsssmsssssnsess PAGE 29. APPENDIVX: CuimlaDiivaerMyorTaolsiitybyPodnyfwiotmh PsRNoOrSther Bobwhiteen Page44 APPENDIVEX: RIndeividDualiBrodTyWoenigyhsS(9y)fwoimhPaRNoOrSt.henBobwhite Pg45 APPENDIX VII: N-oSFnehehdyeCrWosnEBsRuaPmwRpOthSii,oen (Agc/tebirredar/bsdyaTPyoer)nifrom a enn Fog 58 APPENDIX VII: ChantoPgreots... Pagel APPENDIXX: Persone Involved 5 SO. OE6S2 APPENDIXX: Revisd Study Surry: APPENDIXXE: ReposAGRA. en og63 og68 AtenokD n0599 WILDLIFE INTERNATIONAL, LT 7. PROJECTNO: 4sei0s SUMMARY `SPONSOR: 3M Corporation TEST SUBSTANCE: PFOS `WILDLIFE INTERNATIONAL LTD, PROJECT NO: 454-103 STUDY: PFOS: A Dietary LCS0 Study withthe Norther Bobwhite RESULTS": The dietary LCS value for northern bobwhite exposed to PFOS was determined to be 220 ppm .. with a 95% confidence interval of 164 ppm 2.10 289 ppm ai. The slopeofthe concentaton-response curve was 7.005 andth chi-square value was 0.023. The no mortality concentration was 732 ppm ai. Based upon treatment related morality, signs of toxicity and effects upon body weight gain at the 146 ppm ai. test concteh nno-otbserrveda-<ftlecticonocentnrat,ion was 73.2 ppma. TEST DATES: Hat~Acpehl 12,1999 Acclimation ~ Apri 12:22,1999 Experimental Start April 22, 1999 Experimental Terminatio-n May 14, 1999 CNOONMCIENNATLRTAETSITONS: 0,183,366, 73.2, 146,293, 586and 1171 ppmai. TESTANIMALS: Northern Bobwhite (Colin virginianis) AGE TEST ANIMALS: 10 daysofage at est initiation `SOURCETESTANIMALS: ~ W8i59l8dCeomInmteermcateiDornialveLu. Production Flock Easton, Maryland 21601 STUDY COMPLETION: April 26,2000 AMENDED REPORT DATE: March 23, 2004 mSaetecriAalppfeonldliowxiXngfstoudtyhceormepvliesteidonno,minal est concntations and elsbased upon the eanalyss ofthe test AMENDED 610600 ------------------------------------ WILOLIFE INTERNATIONAL,LD 5 rRomCTNo. ast103 This sty was conduted by WildiItNeTIRnOerDmUaiCoTnIalONLid. for 3M Corporation at the Wilde Tematins Li. avis tosslogy fcily in Eason, Maran. Th inf orion ofthe fst vas conducted from Apel 22, 1999 to May 14,1999. Ra daa eneried a Wild neraionl Lid and copy of the final report ars fled under Project Number 454103 in archives located on the Widite Iemaional Lid. se. The objciveof hisstdywes 0 evaliat tOBhJEcCTyIVoEfa st substance 0 the Northen Bobi (Cols irgiianss) administered through the i fr fv days. An LCSO value will bs calculate, itpossibe. MATEARNI DMA ETL HOS DS Envi"rTohnememnettahlodPsroutescetdioinn AcgoenndcucytiSenrgietshis85s0tudyEcaorleogbiacsaeldEfufpeocnts pTreosctedGuuriedselsipneecsifOiPedPTinS tNhuemUb.eSr. $50.2200 (1), Secon 71-2 of the Environment! Frofcion Agency's Regisuaion Guidelines, Pesticide Assessment Guidelines, FIFRA Subdivision , Hazard Balusion: Wild and Aquatic Organisms (2); OECD Guideline 205, Guideline for Testing of Chemicals, Avian Dietary Toxicity "Test 3) and pon ASTM Standard ESS7-87, "Standard Practics for Conducsing Subscue Ditary Toxicity Tests with Avia Species" (4), "Test Substance Wil"Tdhee tIetstemsautbisotnaanlceLuwda.sMreescteiifvieadtifornomNu3mMbeCsor4p6o7r5atpioonnonreOcctto.berTh2e9,te1s99s8bsainadncweaswaassswihginteed powder deifiead:FC.95 Lot No:217. The reported puiyof th est sbsance was 98.9%, with an xiao datof2008. Following est eminatin, the ts teri was reslyzd. Therss of cant indicate. tssubstancepurity of 30.49%.Allesconcenhavrebue iadojusntedsto Sreofrleecdttuhnedpeuraimtybireenpcoorntioeindonthse new Certificate of Analysis (Appendix I)". The test substance was. "Th imal sander ws recived from 3M Corporaon on Jay 2, 1998 and was assigned Wide ematiosa Li. deifcaion mmber 4526 upon ei. The trl standar, granular eeFloslsloowafinmg sttusdy coomo pletmione , the testof6m5a 5.twWae shtrieeXr chariamccta eriazl oed sinetaoccoYhrvdaenoceoeftwitehhGLamPdesitatndatordrs eaned thhee AwenoED C0601 _--_--mmm_ WILDLIFE INTERNATuIDONAL, 9. PROJECTNO: 454103 material, was identified as: 1H, 1H, 2H, 2H Perfuorooctane Sulfonic Acid, Chemical Absiract Number: 27619-97-2. The stanwadsastorreddunderambientconditions. `TreatmentGroups `The test consisted of geometric seriesofseven est concentrations anda control group. Thirty northern bobwhite chicks were asigaed to the control group and ten northern bobwhite chicks were. assigned to cach of the weatment groups. The birds were soried by weight, then chosen indiscriminately from within each represented weight clas for placement ino control and treatment groups. The birds were housed in brooding pens containing five chicks cach. Nominal dietary concentrations used in his study were 0, 183, 36.6, 73.2, 146, 293, 586 and 1171 parts per million active ingredient (ppm a.) ofPFOS. The dietary concentwerraeetstaibloisnhesd based upon known onicity data and information supplied by the Sponsor Esch group was fe the approtpesrtoircaonttoel diet for five days. During the exposure period the control group received untreated feed. Following the five-day exposure period all groups were. given untreated basal dietfo treedays. On Day 8, haofltfhe surviving treatment and contro birds were cuthanized and liver issue, blood, and bile samples were collected for analysis. The remaining. birds were fed basal ration unl Day 22. On Day 22, these birds were euthanized and also sampled for liver weight, blood, and bile. `DurotftiheoTenst "Theprimary phaosfethis test and theidurations were: 1. Acclimation- 10days. 2. Expos-5udaryse. 3. Postexposure observation -3 or 17 days Test Birds All northern bobwhite (Colinus virginianus) were 10 days of age and appeared to be in good health at initiation ofthe test. The birds were oblained from Wildlife Intemational Lid. Production Flock, Easton, MD and werehatchedon April 12, 1999. Birds ranged in weight from18 0 23 grams at test nition. The birds used in this study were immature and could not be diffeentited by sex. noGo2 A-------------------------------------- WILDLIFE INTERNATIONAL, LT "10- PROJECTNO. 458.103 A binds wer fromthesame hatch, pena-ndpchenaatyrpicdalyindistingushalefrom wild bids. Albirds wereacclimattoethde cagndifancilgis rom he day of batch uni iaon ofhe esi `AnimTahlrDoiugehtout secimaton and testing all test bids were fod a game bird ration formulated to Wildlife Inematonl L's specifications (Appendix ID, The chicks were given a viamin supplement hee watefrom th daythywer hitched nil th tationofthe test. Wot from. the town of Easton public water supply, and feed were provided ad im during acclimation and testing. The binds esivendoformofantic medication duringaclimstion esting `DPiretepaTrhaettiesotnsubstance was mine direc nto the ation. Mixing was done with a Hobart mixer (Model Number ASZO0T). Al dietary test concentrtions were adjustetdo 100% PROS based pon thereprted pity ofthe est substance. All dietary concentrations and the LCS0 value are reported 23 ppm a. in the diet. Nominal dietary tet concenrations sed in tis study were 183, 36.6, 73.2, 146,293,586, 117 ppmai. Append IV). Dict`SSaammpplleisngof the test dies were cllcted to very th test concentration administered and fo confirm th sabi and homogeneity of the test substance in th dits Homogeneity of the test ssiuxbsstaamnpcleeisn ftrheomditehtewa1s71evaplpumatead.. bytesctoldlieccttiangrseipxasraamtpiloensafmroDmatyhe0. 18H.o3mpopgmena.eii.tay.i.satmesptldeisetwearned collcted rom th top, middic snd botom of th Ieft an right sections of the mixing vessel. The Homogeneity samples also served as verification samples. One verification sample was collected from the contol dict snd two verification samples were collctd from each emaning ratment roup a preparation an Day0. At the end ofthe exposure period (Day5),one sample vas collected fromthe control and two samples were collected from cach ratment grouptodetrine ibility of the test substance in he diet unde est conditions. The stability samples wer collected from feed remaining in th feeders aftr being a ambient tet pen conditions for fv days. Samples were ransterrd immeditotWeildye Intemational Lid analytical chemist: 0603 ---------------------------------------------------------- ne WILDLIFE INTERNATIONAL,10 _ PROJECTNO.: 454-103. AnalyTthiecalmeMtehtohdodused for the analysis ofth avian diet samples was based upon. methodology eloped stWideItcmaional Li. and cited "Method Outlinefor he Determination of PFOS in Avion Fe, Avian diet samples were extracted with methanol. Methanol was adfodreeidse quantity of feed contained in a Frenchsuare lass bot. Boles wer cpped and shaken on a shaker ble, Samples were vacuum ler sing quali ies pope. Therese fd wsrind thc mes with methool it the Fst, The fe vas tasted o a volumetric ask and brought to lume with mean. As spproprit, samples were furor died wilh melbanol. Each sample then was dtd with S0%4 methanol: 50% NANOpure wate souion contin 0.100 mg 51 PFOS (tema standardyLand0.05% fom cid (v4)so the thy el within the calibration rane ofthe PROS methodology. A method Towchart is provided in Appi I, Figure Concentrations of PFOS in th standards and samples wer determine byreverse phase high performance lid chromatography sing a Hewlet:Pckard Model 1100 High Performance Liuid Chromsogrph (HPLC) witha PekinEms API 100LC Mass Spestrometercuiped witha Pekin Eimer Trboloyrsy fon source. HPLC separations were achieved using a Keystone Basi Cn snlytical column (100 mm x 2 mm LD, 3 um poricle sie). Th instrument parameters are ummariced in Append, Table 1. Calton sandads of PEOS repre ina S054 methane 0% NANOpure wate soto Consining 0.100 mg 4H PFOS (intemal sandardL. and 005% formic acid (vv), ranging in concentrationfom 0.00229 100.0457 mg a1. were analyzed ith he sles. The same and most prominent peak response fo PFOS was uilzd to monitor PFOS in all calfraton, quality cool, and study samples. No stempt was made to quay PROS on the basis of individual isomeric compacts. Linea egesion equations were generated sing peak are response ais (PFOS intemal sanded) versus the respetive concen ratios (PROS neal standard) of the albration sunduds. A typical alaion curve s preseied fn Append I Figure 2. The concentrationofPROS i the samples was dermined by substvuing the peak are response ios ino the applicable tna regression cquation. Represent on chromatograms of low and high albanstandards eerset in Appi I, Firs 3 and 4 respcivey, 610604 -------- ---------------------------------- WILDLIFEINTERNATIONAL,uo meoscriaso eins: a he p`rTohdeumcettohfotdhelilmoitwoesftqcuaanttitiaotinon s(iLanOdQa)rdfoarnatlhyezseedan(a0l0y0s2es29wamsgset1at)1.a1n5dptpemia.i.lcualcuflaoterd oasf he mat lari samples (500) Two matrix blank samples were analyzed to determine possible inerfrences. No interferences wre observed atoabove th LOQ during sanpleaulyss (Appendix I, Tble 2). An rfroce in the ocd speared t spss th ame retention ime as tepek of meres but i was wel below the LOQ. A representative chromatogram ofa mats bak is presented in Appendix 1, Figure 5. Aven dict was fried at 457, 183 and 1830 pm ai. and analyzed concurendy with the wsaemrpelneostocodemteertmionrtthheemmeeaannprporcoesdsucruaelsrreeccoovveerryyo(Afpp9e4.n7d%i.x IA,rTeapbrleese3n)t.atiSvaempchlreocmoantcoegntrraamtoifons mati onion is presented in Append T, Figure An cxample calculation is presented for sample number 454-1032, nominal concntraton of 183 pps navi di. FttiVoglum:e: 210000%m DiPlRuOtiSonPeFaakcAtve:: 101035e68rmedteiin ce ol duton ctor nPaekaAlvSeaRntd:o P0e27k4A9ve: 13160 [Te-- Spe: 277397 Cnucre:s we0i0g1h5e9d415) 1 renin t+ PEAT Fig) x15Cons we 610605 _--mm WILDLIFE INTERNATIONAL, LTD 3 PROJECTNO. 454.103 (02749-0x00.11080m9g). ame = 000923main Not: 5. nemasda. PROS gma mample + S POS (mg 841) at neuenEE Fin ns Vee ((1)0x DiiltnonPFaactor 000923x0200x 100 am = 18sppmai Pere .oro Conceniionon.= I FEOSGIE mOL)inSsD aIple = EE eo HousingandEnvironmental Conditions During acclimation and testing, ll birds were housed indoors in bateies of thermostatically `controlled brooding pens manufscured by Bescon Steel Products Co. (Model No. B35). Each pea had flor space that measured approximately 72 X90cm. Ceiling height was approximately 23 cm. Extemal walls, eiings and loos were constructedofgalvanized tel wire and sheeting. Birds were sorted by weight, then chosen indiscriminately from within each. represented weight class for sssgment pens. Each group of birds was identified by pen number and test concentration. Individual birds were identifi by leg bands. During the testth average temperature inthe brooding compartmentof the pens was 38C 4 2C (SD). Average ambient room temperaturefo this study was 27.3C 12C (SD) with an average relative humidity of 31% = 14% (SD). The photoperiod (maintained by a time clock) was sixien Hours of ight per day during acclimation and throughout the test. The light source was fluorescent Vights which closely approximate noon-day sunlight. The birds were cxposcd to an average of n0G06 _-- WI ILDN LIFTE ERNATtI_ ONA_ L, reomcros sseis . proximately 139 fo of llinstion, Housing and husbandey practes wee based on guidelines `established by the National Research Council (5). ObDseurrviatnigocnslimatailboidns were observeddaily. Birtxhiiingschbaovoaorprhymsiacal yinpjcuarlylwyeoebsenrovteduseatd.leasFtotlilcoewidnalt.et Ainrietcioornd waansd mcaonitnianuiinndgofulntill tmeorrmailniattyi,ons,iganls obfirtdsitweyr,e 2d snare chavo, AnimIanldiBvoidduyalWebiogdhytsw/eFiegehdtCsownesruempmteisonsed Days 15 and Day 22 fr al reining bis, a th inion ofthe et, Averag fod consumption on Day values d 5, i D n ay 5, and on the cxpasre cpearcihodir(aDtamyesnt05g)rosunpdatnhde pthoesscosnpvolsrgsraowb.srvAtdodnipnearilo,d (fDeaydsc6o8n)suwmprteiodnetwearsmidneetderbmyinpeedn foorr Days 15 determined and 1522 for the remsining by messing the chang i he ceament and weigh of the convol bins, fed presented Fd to te consumption ws bis ove 3 given peroiftoimde. Theaccuracy wastageoffedbythe bis, offed consumpio value may hav been affected by th unavoidable Gros Nesey SubjAectetd 0idsgohsstnieccropdsyi. nAhdedcitoionnsalf, vheresstwenrde awleibgihesdesndilnve satuhse,embilnot,ionndwbeirlee werecollectedfom bits cuthnized on Dy the couosfthee dy and 22, and when possefomthose that died during `StatiMsotircaalliAtnyadlyasaeswre ante in he computer program of C. Sepa (6). The program ws adevseirgangeedmteothcoadlcorulthethebiLnComSiDalvarluoasndatheme9t5h%odco(n7f.i8d9e)n.ce Iinnehviaslsbtyd,protbiet LanCaSlyOsisv,amsovwiangs determined sing the prot method. Sondnes of fi est wc report, The lopeoftheconcenzaton sponsecurveand esasofhe Body weight dota were compared by Dumnets test using TOXSTATsofware(1011). No sasica antes were spl 0 cd consumption da. 610607 - WILDLIFEINTERNATIONAL, LTO PROJECT45N410O3 : 1s RESULTS ---- Avian diet samples were collected from the 18.3 and 1171 ppm ai. tet concentrations and analyzed 10 evaluate homogeneity of the test substance in the avian dit, The analysis of these Samples also served as verificationoftest substance concentrations. Resulting mean measured concentrations, sandard deviations and coefficients of variation (CV) for these test concentrations Were 19.5% 2.13 ppm a. (CV = 109%) and 1196 + 702 ppm 8. (CV = S879), respectively (Appendix TT, Table 4). Control avian diet samples collected ding th test shnooinwtereferdes above the LOQ. Samples collected during th est 1verifythe 36.5, 732, 146, 293 and 586ppm a. {est substance concentrations had mean measured concenetons of 402, 745, 174, 291 and 537 ppmad.,respectively. These values represented 10, 102, 119, 59:3 and 91.6%ofthe nominal concenratons, respectively (Appendi I, Table 5). Analysisofavisn die samples collected from oeder ft being held at ambient tmperstur or five days sveraged 101, 122, 104, 101, 109, 114 and 102%of the DayO valvesforthe 163,36, 72, 146,293, 556 nd 1171 pm a. test substance concentation, respectively (Appendix I, Table ). A representative chromatogram ofa test sample is shown in Appendix I, Fire 7. One incidental maraity acer i th cote group din he coursofth std (Table 1 and Appendix V). On the momoifnDagy , ane bid was noted wibtrokhen leand was subsequently cutbanized on Day. Additionally, wobirds nthe control group wer intritentynotedwith foot Jesions sociated with cage mat aggression Otherwise, ll controlbirdswer:norma in appearance andbehavior rovghouthe test No treatment ested mortals or overt signs of toicty were observed in the 18.3, 36.6, or 73.2ppmai. westment groups. One bind inthe 18.3 ppm ai. restment group ws ned3s lame fom Day 6 though Day 8, and blood ofundetermined origin was noted on the undersideof onebird rom the 3.2 pom a. treatmentgrouponDay 22. Osherise,al bids in the 18.3, 36.6and 73.2pom a. treatment groups wer normal in appescance and behavio throughout the test period . 0608 WILDLIFE INTERNATIONAL, LD "16 PROJECT NO. 454-103 There was 11% (1 of9) mertality i the 146 ppm ai. treatment group, 80% (8 of 10) mortality in the 293 ppm ai. treatment group and 100% (10 of 10) mrtality in the 586 and 117) ppm a. treatment groups. In the 146 ppm ai. treatment group, one bird was euthanized on Day 3 ater sustainbrionkegn leg This incidentalmoralicywas not ued fn the calculation ofthe LSD value. Additionally, there was ontreatmentelated morality in he 146 ppm a.. wament group, @ bird found deadon themorningofDay 7. Clinical siofgtonxicsity wereobservedinthis retment group on Day 5, when two birds displayed wing droop. Al ober birds at this test concentration were normalinappeaarndabenhavcioerforthe durationof hetest. Tn the 293 ppm .. treatment group thee were ighttreatmentrelated morals, occuring on Days 5, 6 and 7. Siogftonxicsity were first observed on the morning ofDa4aynd continued fo be exhibited through the morning of Day for he single bird euthanized on Day 8, and through the afermoon of Day 8 for the single bird surviving ul Day 22. Signs of toxicity included a ruffed appearance, reduced. reaction to simul (sound and. mation), lethargy, wing droop, loss of coordination, ower limb weakness and convulsions. The single remaining bird appeared to have recovered and was normal in appearance and behavior fom the aflroon of Day 9 until test termination. nthe 556 ppm a.. treatmentgroupmerlewer frst nootnDeayd3an contintuoebde bserved through Day 7,atwhichpointsl birds hd died. Overt signs of toxicity were frst observed on the affemoon of Day 2 and continued through the mori of Day 7, when the foal birds were found dead. Signsofoxciy obsrved among birds in th S86 ppm... eatment group included fled appearance, reduced reaciontostimuli (sound and motion), lethargy, depression, wing droop, Toss ofcoordinaotwieolnim,b weaknes, lowe im rigidity, prosrate posanudconrvulesio,ns. In the 1171 pom .. treatment group 100% mortality had occurred by the mornin of Day 4. Signs of toxicity i the 1171 ppm .. treatment group were first observeond the aftermoonof Day 2, with the fist mortalities noted on the morning of Day 3. Signs of oxiity observed prior to death included a ruled appearance, reduced reaction to stimuli (sound and motion), lethargy, depression, 1sofcoordination, wing droop, and lower limb weakness and rigidity. 670609 WILDLIFEINTERNATIONAL,LD Ra rORCTNO:assis `BodyWWheeinghctomapnadrFeedeodtChonescuomnptrtoilognroup,ther wer spp reatment elated ef upon body welght among bis in th 183, 36.6 or 722 ppm a. treatment groups. However, here ws a concentration espaniv eduction in body weight gaio body weight los i he 146, 293 nd 586 DPiPfMfera.ein.cetsrefatmeinhteocgroonumtposl dgurroiunpgwethree esxpoasureaperSiiogdni(fDiacyasant0-p<5)00(T5ablfeor2thaen1d46Appppmenad.ix vVIe)l. 20st p00 fr the 293 and 586 pom ai. eels. A snisicaly significant (<0.01) rducion in dyweight gin coniued obe cbscravehde146pmai. st concntraton trough Day ofhe Study. At the 253 ppm ai. consenation a sata significant (p<001) mean weight loss continued through Day8 ofthe study nd a masked roduction i weight gan was noted through Day 15ofthesty. Ductooamorabloidytweyig,htfectcould tbe determionrethd 1171 pom 3 evel during the exposure pericd or for the 536 sod 1171 ppm, ream groups or pos exposes prod her wer 0 span eament elated fst von ed consumpion a he 183, 36.6, 73:2 or c1o4n6supmppmioan.i.watessttceondcentttrahteio2n9s5,(T5ab9lea3ndan1d171Appppenmdiax. VtIrIe)a.tmeHntowgervoeurp,s ra irnedguctthieonexipnosfuereed period (Days 0:5). There were no eamenteae fects on fed consumpion fn any of the Suiviog samt groups durin th Day 68 postxposure period. Inte 293 ppm ai. vesment ar.outetocononcnebnyiartdonsuurrivngib0voDteahdyth2e2.DaTyhe1e5ducanidon15in.2f2pedossonxspuomspionpoebrisoedrsvewdaas tthhee 2e9l3tppomf having any ane in inthe es, and was otconsidered ob reams ele. `GrosDsuNreicnrgotphseycour of th est, all bids that did were subjected 10 8 gros necropsy. Necropsy ress for bids found desd were smi, Common absrvtions included thin condi, 10s of muscle mass, aired spleen color, autolysis of sues and pale organs. Details of the necropsy ndingsar prsentodin Tabled. Halfofhe surviving birds ers subjected 10 gros necropsy on Dey 8 and the remaining beds were neropsied on Day 22, followin tst trminaton. On Day , on bird inthe 73.2 pom ai resent groupws td with lig pal vr. Dus othe soled natureof his fining, was 600510 WILDLIFE INTERNATIONAL, LD ise PROJECTNO: 454-103 not considered to be related to treatment. The single bird uthanized from the 293 ppm ai. treatment grwaosobuserpved at ncropohsavye witha lackofmuscle mass andgeneralthinness. Sin these: findings comelated with an impact upon body weight noid at his concentration, th findings were considered to be treatment related. Necropsy resus wre unremarkable for all other birds euthanized on Day8. Similarly, Day 22 necropsy findings were unremarkable oral birds. CONCLUSION "The dictary LCS0 value for northern bobwhite exposed to PFOS was determined to be 220 ppma. with a 95% confidence interval of 164 ppm a.. to 289 ppm ai. The slope ofthe concentration response curve was 7.005 and the chi-square value was 0.023. The no mortality concentration was 75.2 ppm a... Based upon rcatment related morality, signsoftoxicity and effects upon body weight gain at the 146 ppm ai. a test concentration, the no-obscrveds<ffet concentration was 73.2 ppm a. 670611 ------------------------------------------ WILDLIFE INTERNATIONAL, LT "19 PROJECT NO: 454.103 REFERENCES 1. U(.dSr.i)E,nOviPrPonSmeNnutmablePrro8t5e0c.t2io2n00AgAevnicya,Di19a9r.ySTeorixecy$5T0os-tEcologia EffTesetGucidetlinses 2. USEunSbv.diirvEoinnsvimioencnoanEl.mPernHttaeazolairoPdrnoAtEfevecaltauieaoytn,ioOnA:gfenccoyW.fiedls1f9ce82i.nedrPoeAsgtqaiumcaistd.iecAWsOaerssgshamgeniniot.nGuDiS.dbCesliencesso,nFI7F1R5A 5. OTersgta.niOzEaCtDioGnufiodelEinceonoormiTecstCiongopoefCrhaetmiiocnalasn.d DGeuivdeelloipneme2n0t5., P1a9t%s. Avan Dietary Tuscy 4. `AASmSuebTarcMiuctSaeanDriSdcoaacrriye,tTyVooilf.coirt11yT.0eT4s.etitnPghwiiatlhndaAdvMeialtserSPipoaclisc,s. A19S87T.M SSitnanddaarrddEP65r8i7c.e AfonrouCsotnBdouockinogf 5. NWaatsihoinnagltonR,esDe.aCr.chNatCioounnaclilA.csd1e99m6y. PreGsu.ide12f4oprp,the Care and UseofLaboratory Animals. 6. SCtoemphmains,sCiLEo.n 1978. US. EPA, Environmenial Research Laboratory,Dulth,MN. Person 7. SHPtaeazpahcradhn,aECv,aEl.uPaot.1i9o7n7s., MAmeriecanf.torSoCcahilectlyatoifnogrdaTnesLtCsiSn0g.sFnadgMsa5t.e94sI.n Aquuabt.ic NToo.xicSoTloEgy6a5n4d 8. Finney, D1. 1971. StaMetthodssiniBllcoicaalslsa, 2nd. GriffinPres, London, 5. Thompson, WR. 1947. Bacteriological Reviews,Vo 2, Noz: 115.145 10.wiDulmCaeotn,eCoWl. Jo1u9r5,5.AmeAr.MuSlati.pleAsCsoomcp5ar0s:n1'0s96P1r.3o1c1edureforComparing Severs Treatments 1. Gulley, DD, 1990 TOXSTATRefs 3:2.To UniveofrWysoimitngy. 6n0612 --_-- WILDLIFEINTERNATIONAL,10 = phoseeraosso:s TABLE| Cumulative MorfaomlsNiotoyr Bowie Ace Dicary Toxicity Sudy ith PROS En Toros Fores owBiFos = Grmai) ORO BRT wT AwT BTC BT y owe DT DT Conet . wo wo ow om om we wo wo um Treament ws 306 na oo mo ow wo mo oo ol m0 on ow wo ow ow on wo wo wo aw wo on mo wo on ow oo om wo 146 BY 10 wl 0 or 00 om 10 on 09 ow 0 20 09 wo w"ow w"wo st ol om om wo wo so wo wo wn ow om on wo wo wo wo wo wo d TTT Eiy hT ayyRT ee mae eT eT 610613 ------------------------------ WILDLIFE INTERNATIONAL, to La. PROJECT: 454103 TPAaBgL1eE2 MeanBody We8i)frogm aNhorthtern Bobwhite Acute Dietary Toity Study with PFOS "o-e_rm--o y eExpoenueporng Day0 Days Day0s PostExposeGPoensieod Toul Day8 Day 5:8 Change' ContTrol kMyem 2Tomnw s x HI s i Treatment wo MBye owTh ws u ws2 3 66 kMye m> w5hon wo 0 w3 no M5em ow[ESws woTo 126 wo 5wen m2 awTd w Ts e i3 wm =Mem 2TTae a3 rvo a a5e 6 5Mem 2ToTae ae Lo oo H. r ie ra le5Med iyfw Toe reLeno oyws SRSe . y (:.5 Are :. SOCaRldyn y Ba centot thomeoeira)mtgroo tp<00005 Dv oe's s) 6n0614 _-- WILDLIFE INTERNATIONAL, LT "z- PROTECTNO; asa103 TAPBagL2eE? Mean Body Weigh (9) foNortmher Bobwhite Acute Ditry Toxicity Sty with PFOS TEroer --_-- Ee Goer ---- GE tw Dus Duis Dutis own pein ows Cou"rt Moe weoh on3 2i 23 u:s Trosmen woe5 0S5 e3 w w7 on3 o7 66 M3en wE e x PE3 3 5: nom=e 3EeA w n : w2 ": woveByn wos8Aoa n 2 a2 us: ?m em ow kw sow u S56 w5en S To L B .: .: E s Wi t MFsen ae lyLooDd owrox e : ei: r ad i: V Smsd in opa e coiy pap 005 ous 600615 --_---- WILDLIFE INTERNATIONAL, (10 a. PROJECTNO: 454.103 TPAaBgL1eES Mean Feed ossurmpion gToixdiidtyyS)tudoymwiatNhorPEtGhSe.r Babi Acute Dicry --G--om--n) Expo and -- Dues o EySen s: Tresment ws 5 6 . na 0 46 5 5 56 . wn . Pot pDounersPeriod 0: u u i 0 ' . T e------R -------- E ------E ------ Gn0G16 _---- WILDLIFE INTERNATIONAL, LTD a. PROJECT NO.: 454-103 TPAaBgLeE? 3 Mean Fecd Consumption (Tgobxiircdi/tdyayS)tufdryowaimthNoPrFtOhSem Bobwhite Acute Dietary ------ (pGprmouaps) PostExposure Period E CC r -- o sDMe.an 02 51 Treatment 183 366 n2 146 ES ss un 0 2 1 1s 1 1s n 1 5 5 - - Tm CrOGL7 _-- ST WILDE LIFE TITNETRERNNAATTIIOONNAALL,,oLD E'S PPRROOKJECCTTNNOO.:d4s5a4.a1003y Group GroTssAPBaLthEol4ogical Observations from Norther Bobwhite Acute itary Toxicity Study with PFOS Birds that died duringth course ofthe study PEMArr Finding Cowl TW HE mI T _ MNeit NNw2 NN%= NNeewo NNeeoo AAbbddoommiinnaall ccavviityy,, asuotmoleysaitsltyhsriosughout o o 0 2 0 o 24 1 1 ECmrcopi,neemdp 00 00 2 2 s 2 5 5 FGlr.acuarced clemg p o1 o1 0) 0| o0 GHeiazra,rdatcoenitoepnorbtiieonsminoelde white color o o o o 2 1 5 0 1 0 Hear, pre Intestinal contents trike o 0 o o 0 0 2 1 2 0 Keel, prominent Kidoeys, ple 0 o 0 i o 0 5 10 20 LLiovseorfpmiluescalned mmaostsled o 0 | 0 h0I 07 90 MSmuaslcloilnasrstkeelel pa 0o 01 03 00 0o Spice, black Spleen,dk o o o o 0 o | o o 2 Spleen, grey Spleen, grey-brown 0 0 o 0 0 0 1 0 0 1 Spin, pre Spleen, small 0 0 0 0 ' 0 o 0 ! | Spice, Thin small nd pale 0 0 0 0 0 0 3 o 4 2 e Not Remarc 0 e o ' 0 o 10618 I TT --r------------------ : 1 : he : i $855535 53 55 55 2% % 3% 3 25 t 2 %25 2 8] s85528 358 338 83 22 $5 3% 33 : #18 ssss525 53 55 55 3% 23 %% 33 i i 838338 353% 83 8% 22 8% ayy 2 Hl | 233 Pi 8883383 33 33 33 32 83 5% asf i | feliz 833833 38 38 38 52 Eg Eg Ay | S583 3 = i& SY57 sessss 5555355353 33 33 337 1 z 1:|: 22 52 55 5 38 548 i BI p5 = 1 dlz z 888555 sg 55 S288 55 55 gE 5% es 3% SF 33 84; = 1 i 13f d MBE EEEEEECE REECE ER ERE HH 9H Bf cnerne mon en me eee of1ffaEi2lE 3z8 Lios2k: coccso3p 33 r 55 Hr N a$2a A55 Es 32R3 Ei E s E R g3l & E EER 3 610619 - WILDLIFE INTERNATIONAL, LTD -27- PROJECT NO.: 454-103 Certificate Of Analysis ies, 00 FC-95, Lot 217 Pp urtas ont srtonnctr rem. re Hi - pees F eee r---- d dw oso m ) Addon,nme rionftp adetec ios ENMRsckeiquensnd undto GR0G20 WILDLIFE INTERNATIONAL, LTD "ae PROJECT NO: 454-103 APPENDIX 1 `WILDLIFE INTERDNIAETTIFOONRAMLULLTDA.TIGOANME BIRD RATION' INGREDIENTS FSWihoneeaBCteoaMmnidMMdeesaall, 48%Procin PArgocaiyn BSpaesceial, 60% Protein `DArTied WMheeayl,20% Procin GEMeartsohtuimnoadnniLnCieamlerPshteoosnie + Liquid VGSiaLtlaFlmeoidrnimzae(ndFdeMrimnaetrcaol)Premix se below) Toul PERCENT (4) a36055650 0000 320500 000356s00 0023i5r} Woo VITAMIN AND MINERAL PREMIX AMOUNT ADDED PER TON `Viiaamiinn ADy RNiiPbaaoncftioltnahveinnic Acid VFoliicmAociBdy BiPoytiidonsine TVhiiaammiinneE VMiatnagmainneKs(eMenadions Dimethypyrinidine Bisuic) CZoipneper ToiSdeoilnenneium 270000000000011C.U. 0160 gggrrraaammmsss 008 mggss 12megsums 200010210grams 10528grgarmasms 47Eggraamsn 1581125gmrgagamsms ThoefgSuharcarnutdeeedfiavnear.lysis i minimum of 27% proc, a minimum of 2.5% crude ft and@ maximum "Fementaion By-Products (Source ofUnidentified Growth actors). C2622 _-- 9. WILDLIFE INTERNATIONAL, LTD PROJECT NO.: 454-103 APPENDIX II ANALYTICAL METHODS ANDRESULTS 6noG22 _--m---- WILDLIFE INTERNATIONAL, LD 30- PROJECT NO: 454-103 INSTRUMENT: ANALYTICAL COLUMN: OVEN TEMPERATURE: sToPTIME: FLOW RATE: MOBILE PHASE: INJECTION VOLUME: PROSRETENTIONTIME: IRENTTEENRTNIAOLNSTTIAMNED:ARD. PFOS MONITORED MASS: _MION--TNEIRTNOARLESDTMAANSSD:ARD APPENDIX Ill Table 1 Typical LMS Operational Parameters aTTPueerwrbeokrli-onPnEaSlcpmkreaaryrdfAoPMnIosod0ue0rcLe1.C10MO0paeisrsgaShtpeePdcietnfrsooermlmeeatcnteicrveeeqLus1ioqnpupmdeodnCihwtriootrmhimnagmPomegokrdiaenphEwlmier (SM), Keystone Beasl Ci cour (100 mum x 2 mun LD, 3 um patie size) soc 100minutes 0220 mUminse 72.0% Methanol 28.0% NANOpure Water containing 0.1% Formic Acid 200 Approximately 7.0 minutes Approximately 48 mines 498.6amu 267 am C0623 -_-- WILDLIFE INTERNATIONAL, LD re PROJECT NO.: 454-103 APPENDIX Ill Table Matix Blanks Analyzed Concurrently During Sample Analysis giNsea-m1i0e3:) eee Tipe mePROm S! e Gpmai) MAB-1 `MatixBlank <iq MAB2 TihnealyT2o2dto(F0q00u22a9 nmgto1()LOaOn ) Matrix Blank hwaesi1a7i5nptpo1 obfatsheedmpoan 5bapsrsoadmpsleosF <1oQ T50e0)owes caries Samed" : 60624 WILDLIFE INTERNATIONAL, LTD -n- PROJECT NO.: 454-103 APPENDIX TI Tables `Matix Forifcations Analyzed ConcurrentlyDuring Sample Analysis T `Sample Number em omT as percent (asa103) Fortes Messed Recovered MMAASSIAA ias5 iasnt 5103s MMAaSASS2 ji1 171662 98631 MMAASSSS 11583300 11m 576 396317 `Sundard DeviVtiieown == E69R0 oNv=e 2358 "Vo nRedpamlasnu3anldccalocmuelactiioonnss. foVralnueews TheatvesbuebesnnrcoeunpduerdlfyowperoeriGenngerpiurepdosweisng MacQuan version 15 sofware CnOG2S5 610626 Gn0G27 . oo CrOG28 C0629 29630 WILDLIFE INTERNATIONAL, LTD ER AppENDI1X PROJECTNO: 454-103 10 10 aan:1995 on o o 7 o 5 " a 2 as laze 122 o aTh 6S1h 0S1h 12s1e 1a81k 1781a 21w1m TSeeaen Figare3. A rprscttie on chromtogamof sowevel (100229 m 1/1) FOS sandrd 610631 WILDLIFE INTERNATIONAL, LTD 9APPENDI1X PROJECT NO: 454-103 to 188 onal: 26054cps o a 7 & 5 " Ls 2 ' 9 28T Tw 4e 8 2%k 144 eSrm Juszse issTw A7B719020a4sl_ie 230 Tseaen Figured. A representative ion chromatogramof a high-level (0.0457 mg a.i./L) PFOS standard, - 620632 _-------- WILDLIFE INTERNATIONAL, LTD ~0APPENDIiXt PROJECT NO: ds4103 100 wf a a 0 s0 50 "0 . 180 ntoe25t0yc:ps 100 as 4 i 216 TA il aldi 0 e 7WLJTAY ul|Pi2V03)l0 TToET3s eSTn TGak iGeR RTeaiwm STaene FiureS.A"rTehpraersreontwaitinvdeiccheomtshettoegnatmioofn ime oifxPbFaOr.ksale (454-100-MAB-1). Gn0533 WILDLIFE INTERNATIONAL, LTD an APPENDIX 11 PROJECT NO; 454-103 To 188 Intensity:6238cps s 8 7 8 5 4 3 144 2 1 o 24 317 e191 122 151 151 181 211 Scan 1% 25% a; 50s 632 757 683 Time Figure6. Arepresentativechromalogram of amari fortificationsample(454-103: MAS-LA). 670634 _--mm--m---- WILDLIFE INTERNATIONAL, LD -aAPPENDIIXt PROJECTNO: 454-103 - 187 ntensty:7248cps o 8 7 s 5 4 s 2 145 ' 24 123 7 220 S1%TE25% Taet8 asos a6s3t2 a755 esnss Smne Flare. A representative chromstogramofa es sample (454-105.2) C0635 _-- NWITLDELRI=FNEAITNITEORNNAATLIO,NAL, uLT0o a. DIEATPPPREENPDAIRXAITVION PRPOROJJEECCTTNNOO:. 445544-110033 `Weight and volumeofconstituents use toprepare test diets: ConNceonrtmraatlions | STubostrance | Raantiaoln w1o83 018=18 8590909080 37626 00376328 5999993 124063 2L0615253 81999978.15 i586 L58e2i3s9 88998984.24 Diets were prepared as follows: #50000gofbasal ration was weighed into a tared Hobart mixing bow The test substance was weighed in aed weigh bot. `Appbrloexnidmerately 100gofbasal ation was aken from the mixing bowl and placed in a Waring The"wittehttshuebrsitnasnecaelwsoasbeaidndgepdltc0etdheinlethndeblrenadnedr.the weigh bost was rinsed with additional ation, mTihxiebnogbwll.endTehrecobnlteenndtesrwwearse rbilnesneddewdiftohraadpdpirtooxniamlataetlioyn6,0wsietcotnhdesrainnsdetarlasnosbfeeirnegdptloatcheedmiinxitnhge. bol +The"Tbheowrlemwaaisnipnlgacreatdioonn 5a5 Haodbdaerdt fmoihxeerbaonwdltahendcotnh tceonnttsewnetrse wmeriexmifxoredafpoprraoxximmaarteemsliiyxnmuisnus. The diet was transferred 0 abled paper fd bag. 620636 I BEST COPY AVAILABLE .| 5 Tieli xxxxSx REEEERSSEEEEE 14 oi A i 4 5 ensees sessazassssaas irpdi 10637 WILDLIFE INTERNATIONAL, LTD PROJECT NO.: 454-103 ase BE Grow pm 0 APPENDIX VI Individu`aAlcuBteodDiyeWteariyghTtosxi(cgi)tyfrSotmud2yNwoirtthhePrFnOSB.obwhite Page! Bid Dw Dw ChaDnwgOeS. Dwi CDhuansgse. 2' I2n 22 7s 324 73 35 22 22 1i0 wo2 05 Me5n 221 5 i1o6 E') 13 sD 2 4 5 3 -- Toul Change. i5 2i1s zis 7 o 21 E1d 22s 75 338s 05 11s6 33 22 3 15 Fi3 05 27 Me5n T231 3 55 33 93 7in sp i 3 2 3 1 2 0 2' 21 2x s4 23 37 n5 33 22 520 55 3 73 I2 Me5n 321 23%5 53 3 5s i5t sp 1 s 3 s 2 s 0 21 119 2n 39 33 85 1I6n hl3 21 320 1i 32 105 2" Me5n "220 E3n Tio 3B 103 a0 sD : 3 i 3 2 2 Gn0G38 WILDLIFE INTERNATIONAL, LTD PROJECT NO.; 454-103 a6 APPENDIX VI Individu`aAlcuBteodDyicWteriyghTtosxi(cgi)tfyrSotmudayNwoirtthhPeFrOSB.obwhite Page? --Gro-- w Change Change Toul gpm Bird Deyo Days DwOS Ds Dn3 Che o :! 0m zx 79 33s6 57 11s i 50wa 02 1n0 ": 22 2> Me5n "_20 ED3 IiHo W"o 100 2Ts 5| 3 2 5 2 4 0 x1 o1as 30 1n 3 15 22 i 50a 0m 22 ni a5 55 0is Me5 n _ I2 22 In0} a2 15 E2n Gow Mew 202 E2 02 35 i 33 Toul so | 4 5 s 2 4 670639 WILDLIFE INTERNATIONAL, LTD PROJECTNO: 454-103 are APPENDIX VI Individual Body Weights g rom a Northen Bobwhite Acute Dietary Pages Toxicity Study with PFOS. --_---- Gfomin) Bit Duo Dus CDhaonsge pws DCuowngies cTmowe 10 Loew Tm . 1s 2 ow ow uw ow . n sow ow ow s mw on won PF) som ow uw ww P) Me 5 Ti HAT4 Rs y2 3 13 Co om sm ow 1 2 wom sw 5 1 sow ow som 5 1 som wu wow n som ow on ow ' Men W 2 TER5T w 3 3 w ; 5 TToou Vseor H i w 3 s :5 03 60610 WILDLIFE INTERNATIONAL, LTD a8 PROJECT NO; 454-103 APPENDIX VI Individual Body Weights (g) from a Northern Bobwhite `Acute Dietary Toxicity Study with PFOS Paged TEGroup Change Change: Toul (ppm Bid Dayo Days Day0s Day8 Days Change 366 1 18 % 8 3s 9 " 2 1 30 n 3 9 E 31 2 10 3 s 18 +2 0 93 8 1" 5s 2 El 3a 9 2 MseDn 1 203% 2 3 9I 12B 366 ToGruolup ' 19 10 a i 2 19 2 10 3 s 1s 32 2 u "0 3 19 +2 3 3 " 9 2 s 2 3% 3 " 8 2 M0a)a n 2 213% 2 w 4 o 2 SMDen 2z0 33 Tm2 393 ) 52 60842 WILDLIFE INTERNATIONAL, 10 o- PROJECTNO: 454-103 --_oGm--oew--) -- na APPENDIX VI IndividunalcBeodDyiWceigThtoss(e) fSoomdayNworitheFrFnOBSobwhite Peas Bi Dye Dus OChwanegse ows oGuwnets cToeul Com x some ow x 1 wa wo sow ox ow 1 Pp som mom wa i soa ow sxe is MEeAw T 3 E : 3 E : 3 2 [I sw a 6 2 om wom ow a 1s soa ow sw a w om mow wa In sow ow wow 5 So S MSa n2www-- ; w3 77: "; we St Fa 3:3: 3 610642 _-- WILDLIFE INTERNATIONAL, LTD -s0- PROJECT NO: 454-103 TeGeroup (pra 146 --_ APPENDIX VI Individu`aAlcuBtoe dDyieWteariyghTtosxi8ci)tfyrSotmudayNwoirtthhPeFrOBSobwhite Page 6 Bid Dayo ' 19 2 19 3 E) + zn s2 SMDen --2 212 Days ChDaunogse Day$ 23 9 = 0 u "+ 3 n 28 s 3 3 3 83 3s 2 3 2 3 Ww3 ChDaunygSes 7 4 s " 51 CThoaunlge 16 n 16 9 5 w3m 14 1 18 % 3 7 3 2 19 7 8 3s 3 16 31 2 83 6 1 "2 - - - - . s5 u ' = + 5 sMDan T 2 2 63 m wm3 6 2 m 5 BTE oul sD 2 3 3 3 2 4 TO"StatisNtiecalmlyde ifeen romm he control group at p< 0.05 (Dunner's vist). "Statelydiferent fromtheconvo groupai p <0.01 (Dumnen's ics) GnOGBA3 -_-- WILDLIFE INTERNATIONAL, LD si PROJECT NO. 454-103 APPENDIX VI Individu`aAlcuBteodDiyeWtaeriyghTtoxsi(cgi)tyfSotmudayNworitthePrFnOBSobwhite Page? TeGeroup Gpmai) Bi Dwo Days ChaDnwgOeS Days ChDaungse CTuouel EY |1 0 2 2 | 3 2 ow uw 2 . . . sow i 2 - . =m - . 2 . . soa 2 . - . _ Me Tm 2 m5 a a2 T = : : F) ' 1 1 0 15 + 4 ES - - - > . sw 2 o . - - iw n 3 - . . soa 1 5 - p . BE Mao TB H1 Ww2 a 72 - a TEoRul sb 1 2 2 4 i s Stmistcaelly ddrieetftoo behecconotonltgroop 5p<.00.0051DiDcot'ess)). n0644 _-- WILDLIFE INTERNATIONAL, LTD PROJECT NO.: 454-103 se APPENDIX VI Individu`aAlcuBteodDyieWtaeriyghTtosxi(ci)tfyrSotmudayNwoirtthhPeFrOSB.obwhite Pages _E--xpe_rim--entmm pGrmoup Bid Dsy0 DayS CDhaayngoeS Days ChDaanygSes CThoanugle st 1 in - . - . e 22 1 a - - . 32 a - - . 42 - . s 2 " Kl - - . MseDo TW H2 3 2 F : a 5 ss ' 1 16 5 - - - 2 - - . . 5 1% "4 . - . .1 - - . . s2 - . . . . sMpe.n TH| 0 1 : : TGomuls MSeDm. Bi ee2 a2w : : 2 TO"Smiesicmllymdiaemfm o hey cnr group ap< 001 Donne'esst 620545 _-_-- WILDLIFE INTERNATIONAL, LTD Ls. PROJECT NO.: 454-103 APPENDIX VI Individu`aAlcuBteodDyieWteariyghTtosxi(c5i)tyfSrtoumadyNwoirtthhPeFrOSB.obwhite Page -- gGprmouepi,) Bid D0 Dus ChDaunygOeS Dus DChuansgse CThouulm un ' 1s - - . - 2 2 - - - - - 32 . . " . a2 - . . s2 - - . . . sMaDn T 1 ; H BN : T N A y nn '1 - - . . 22 - e . - 32 - - - - - 42 - - . . - s2 - . - - w Gow MMSeepnm T i o : H - : T - : Toul SD ' A y : : --_--m GnOGA6 WILDLIFE INTERNATIONAL, LTD PROJECT NO: 454-103 sie APPENDIX VI Individu`aAlcuBteoDdiycWteriyghTtossi5ci)tyfiSoumdayNwoirthhePmFOBSobwhite Page 10 E"Grou--p, ---- Change Grange Toul Gpmai) Bid DwS DayiS DwBls Dez Duis: Chae 0 23' z1a 526 127s 2an 2aw1 5P5y 3 zn 54 2 " MsesoDs n e "359 oinaw Txm5 x wir 23 ow55 0 2! 3s3 55 is u 2 33 3" "7 75mis 2 @ Me5n 338 5a 27 75) =2 ais so 3 3 s n s 5 o 2! 20 sie is2 7a i2ss 5 35 23 55 2i n7 25 ias Me5 n 3 @5 27 57 EnY w5 Go Mseon 7s 52 EP)5 20 ELYs Eos Toul rr) s i s i 5 8 H0647 WILDLIFE INTERNATIONAL, LTD PROJECTNO.: 454-103 ss. APPENDIX VI Individ"uAaclutBeoDdiycWteriyghTtsPoa(ggie) fc1Sr1otumdyayNwoirtthhPeRmOBSobwhite ET Grmo ) Bi Dns Duis ChOawnlges Dum pCahalnigen CTohulm 183 Cow sm om as " 2 ows ow ws ow " sos es wom 5 + ws 0m ws om sw on 3 ow om " LoES % s 3 AN73 3 I Group Change C-- hnge Toul Qpwai) Bi Dws Duyis Dyids Dy Dis Chae 366 os oe os osm oa " 2 ws x oa om 2 sow es om ow ax 54 + 0% a mw = as sos om ow was ss Ma TR Bmw wm ss 3 0s 272 a 600648 WILDLIFE INTERNATIONAL, LTO 56- PROJECT NO; 454-103 APPENDIX VI Individual Body Weighs (g) from a Norther Bobwhite `Acute Dietary Toxicity Study with POS. Page 12 TeeGroup. Change Change Toul (ppmai) Bid Day Dsyls Day8ls Day2 Dayisz Change n2 1 2 4 n %2 " 2 3 58 2s " 19 a" 3 6 @ 2 " 1" a 4 39 65 2% 87 2 " s 3 E) 5 i 19 2 MsaDn 353 @4 #2 om3 TM2m 2 " TGermouep Change. Change Toul (ppm a. Bid DayS Dsyis Dwis Diy22 Dayls2 Change 16 ' 3s El 2 8 2 4 2 2 55 2 Ld 2 5 3 - , . - . + E 2 st 18 5 2 57 2 i2 " MsaDn T 3 3 a mW1 2 w a2 @1 "Stasicaly dierent from thconrol group a<p0.05(Dunmer's vis 09649 A ------------------------------------------ WILDLIFE INTERNATIONAL, LTD 5. PROJECT NO.: 454-103 APPENDIVXI Individ`uAaclutBeodDiyetWaeriygThotxsic5i)tryoSmtudyNwoirtthhePrFnOBobwhite Pag1e3 TEgGormosl) Bi Dwo Dws CruDnwgOeS pws bCouutpse uTomuel = oa sw som PM 2 .i . 5 . . . .... . 5 ... . ET Mrye THR: W : w: m ----m -- : ee------------------------------------------ 60650 _--_-- WILDLIFE INTERNATIONAL, LTD oss PROJECT NO 454-103 APPENDIX VII Feed ConsumptionD(ipebtiarryddTaoyn)icbiyyPSetnudryowmitahNPorFtOhSem Bobwhite Acute Page 1 Teme Gn _--_-- Gpmai) Cont --_-- Epowereed PT Duos Pewee Feed Dwar ' 5 2 2 ' 2 3 s 7 . 2 n s ' 9 . 0 5 MEne)n T 2 2 ew 2 GNOGSL -_-- WILDLIFE INTERNATIONAL, LTD 59. PROJECT NO: 454-103 APPENDIX VII Fecd ConsumptionD(ige/tbairryd/Tdoaxyi)cbiytyPSetnufdyrowmitahNPoFrtOhSen Bobwhite Acute. Experimental Group Page Txporure Penna ForExposure Period _-- pmai) Po TT Bwos TT Omer 183 2! 55 1i0 Men Tem 366 21 75 15 Mean 7 52 2' io10 21 Mean 0 EB 146 2' 07 18 Mean 5 wo 2! 5` i5o Men TS sts 2' 65 216 Mean nn 21 -5 Mean 0 5 Gn0G52 -_-- WILDLIFE INTERNATIONAL, Lo 60- PROJECT NO.: 454-103 APPENDIX VII Feed ConsumptionD(igebtiarrdyidTaoyx)icbiytyPSetnufdyrowmitahNPoFrOtSh.er Bobwhite Acute Page Tome Gro _-- (pmai) Control --_-- Bere Foren Pa Due mm ' 0 1 2 0 2 3 7 2 SMDa.n T 22 ym : Ee Groe _-- rma) Po 153 ' Bp TBE 10 Fen Epon ered Tyna 2 366 ' " is 2 ' Bn 1s ue 1 n In -_-- ES ' 8 9 Cn0653 -_-- e WILDe LIFE INTERNAOTIAONLAL,,ILTD "61 PRPROOIJEECCTTNNOO.::443541-01033 APPENDIX Vin CHANGES TO PROTOCOL `1.waTshcelaprrifoiteodcol winadsicaamteentdhedco0lleicntdiiocnatoeflithvaetrbiflreowmiblilrbdesctohlaltedctiedddfurroimngslthsetucdoyurbsierodsf, thTehestpurdoytocol 2t.ubBesl.ooTdhseamprpolteoscowleriendcioclalteecdtethdaotnheDpaaryin8iaznedd Dvaacyut2i2nienrsnownouhledpabreinuiszeed.d mi borosilicate gloss est 3Br.eTfPlheMcet,pthe1r0te0so,t 1stwu8ab3so,sta3acmn6ce,eon7pdu3elr2dit,yto1cg4ih6va,enn2g9ie3n,tthh5ee86enseatwndccoe1rn1tc7if]eicnpattpermaoft.aifnroaloTnymessis0s,.co2n0c,en4t0r,at0i,o1n6s0w,er6e40chaanndge1d2800 4n.otTrheecotredmepedratures from several brooder units an Day 4,7, , and all brooder units on Day 22 were r5.esTthmeeanfttgermoouopnoonbsAeprrvait2i6o,ns1w9e99r.e inadvertently not recorded fo2 birds in the 640 ppm a. "0654 _ WILDLIFE INTERNATIONAL, LTD .e2- APPENDIX IX PERSONNEL INVOLVED IN THE STUDY PROJECT NO.: 454-103 "The following key Wildlife Intemational Lt. personnel were involved in the conduct or management ofthis study: (1) Mark Jaber, Wildife Toxicologist (2) Joann B. Beavers, Director, Avian Toxicology. (3) Sean P. Gallagher, Senior Biologist (4) Courtney Casey, MS, Seni Biologist (5) WillarBd. Nixon, Ph.D. Manager, Analytical Chemistry (6) Timothy Z. Kendall, Supervisor, Analytical Chernistey (7) Raymond L. VanHoven,Ph.D. Scientist (8) Ellen Mank, Chemist G2O655 WILDLIFE INTERNATIONAL, LTD ee APPENDIXX REVISED STUDY SUMMARY PROJECT NO: 454-103 This study was reported using a test substance purity of 90.49%. The test substance Characterization was revised on 9/7/00, folowing study completion, and thepurity was determined to be 86.9%. The Sponsor requested that th results of thi study be revised to reflectthemost recent purity analyses. The results previously presented were simply corected by the ratioofthe two purities ora factorof0.9603. No attempt was made to recalculate analytical standard concentrations or measured concentrations from original raw data. As such, minor errors dueto roundingmaybe present. Attached i th revised summary pagefromth final report and the most recent certificate of analysis. AMENDED G5O656 WILDLIFE INTERNATIONAL, LTD PROJECTNO: 454-103 "64- REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9% SrovsoR. SPONSOR'S REPPRESENTATIVE: LDOACTAATAINODN OAFCSOTUPDOYYF, RTHAEW FINAL REPORT: IM Corporation Ms. Susan A. Beach `EaWstioln,dMIaermyaltainodna2l16L0i1d. ALTLD.BPIRSOJEICNTTNERUNMABTEIRO:NAL TEST SUBSTANCE: stupy: NCOONMCIENNATLRATTEISOTNS: TESTDATES LENGTH OF TEST: TEST ORGANISM: SOURCEOFTEST ORGANISMS: AGEOFTEST ORGANISMS: 454103 PFOS (Perfluorooctane Sulfonic Acid Potassium Salt) PFOS: A Dietary LCS0 Study with the Northern Bobwhite 0,17.6,35.1,70.3, 141,281, 562 and 1125 ppmaii `BEixopleorgiimceanltTaelrSmitna-atAirpor-ntilM2a2y, 1149,991999 Experimental Terminatio~n May 14, 1999 24 Norther Bobwhite (Colima Virginians) `EaWsitolnd,eMIanrtyelmaatnidon2a1l6L0i1d. Production Flock 10 days at est niiaion Too 95% CONFIDENCE LIMITS: CONNOCMEONRTTRAALTIITOYN: CO'NNOC-EONBTSREARTVEIDO-NE:FFECT- 2zppmas 158 and 278 ppm a. 703 ppma 703 ppm ai AMENDED G59657 WILDLIFE INTERNATIONAL, LTO "65 PROJECTNO: 454-103 CE ON e ante ee Cl INTERIM CERTIFOIFACNAALYTSIES ConeAndRrLrsatTCr5H0OsR4MirEmee: 3184 oM A om-- o --T-- REv E---- --e . -- = yr EB gee | iin Ef rinn w AMENDED G006S8 WILDLIFE INTERNATIONAL, LTD . PROJECTNO.: 454-103 Ce NEr E ae TiF mera s stmcomapenre Eon stn vrae--: i ire ieThEsTe e TrT e CTA oAgE-------- ------ rnin mr Sm BoeEmp LEENTEEm, ELnmsE.mIEEt pan. ra---- ------ ------T ----A-- T--_ cn rere GNOG59 WILDLIFE INTERNATIONAL, LTD PROJECT NO.: 454-103 OuN rna cINE rRCnOaRcTe enIOLFe r CAVAALTsSTete po s e e5 s g- e t : . EEE TCS av devin Ohwi dacs rp vir -- Hh o-- m al _wEghspsldengh won Gn0G60 --_--_--mm----,--,---------- WWIILLDDLLIIFFEE IINNTTEERRNNAATTIIOONNAALL,, LD ~8- PPRROOJJEECCTTNNOO:: 445544--110033 APPENDIX XI Report Amendment 1 AOmreignidnaeldReRpeoprotr:t: TThielae Pagme ereponrtdadtewaesaddded.Thetotal umbofepagres Reason: wTaoiscnhdiacnagteedtfhraotmth r6e21p0o.6r9.twasamendedandnote changein pagination. 2 AmOreignidnaeldRReepporotrt: TPhaegaem2endedreportdtc was added andthe exceptions 0the Reason: "cToomspliathncheeasomateenmwdeendt wreeproertredvaits,edprnoewvsigniaturdesaend dsaattesmeonrtt.heamended report and tupdatethe compliance 3. AOrmgeinndaeldReRpeoprorti: PThaeges3uit desforth amended report wereadded. Reason: "sTiognsahtouwretahnedadmaetndfeodrrtehpeoarmeanuddeiddartepeosrta.ndto provaindeew 4 OArmgeinndaeldReRpeoprotr:t: Reason: NPaegwessignatures and dates wer affixed anatdl change was mTaodper.ovidesignaturesand datesof appforr oth vameandled reportandtoupdamaanatgere's tle. 5. OArmgeinndaeldRReeppoorrt: PAapgpeendix X, Revised Study SummanadArppeynd,ix XI, Report Reason: "`TAomreenvdimseentth,e wTearbleeaodfdCeodnt0entthesT0abilnecloufCdeonthtenrtesv,ised summary and report amendmen. 6 OArmgeinndaeldReRpeoprotr:i: Reason: PAagfeootnoi and the amendedreportdatewere added to the STuomdmiaertytpahger. etaoAdppeendrix X, which contthaerievnisesd study summary and 0 show the amended report date. 7. AOrmgeinndaeldReRpeoporrti: Reson: PAafgoeostnote was added tothe Test Substance txt "rTeocdenitecCerihfeircaetaedeorftoAnaAlpypseinsdix X, which contains the most AMENDED 610661 CrNGse2
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