Document 70Oded6N1Yr14LGKn7japOV86

AR 226 1337 MR 267555 REPORT 96-HOUR FRESH WATER ALGAL GROWTH INHIBITION TEST WITH T-4127 SZ a3n3 a52 Z=38o8 ~ a NO'NTOOTXOSXubPrsotjaenctce331413356107 Page 0137+ = oid Tatzr STATEMENT OF GLP COMPLIANCE NOTOX Project 334351 NOTOX BV, `sHortogenbosch, The Nethariands Tihne suadsescrroibcead on ios roefport ha been coroct aported and was conducted in complance The OECD Principles of Good Laboratory Praics which ar essential in conormiy with: The Unie States Food and Drug Admiistaton. Tie 21 Code of Foderal Regulations Part 5. TRehgeulUantiitoendsSPaatrs16E0n.vironmental roection Agency (FIFRA), Ti 40 Codo of Feral `RTohceuUlnailtoendsSPtaatres75E6nvironmental Protection Agency (TSCA). Title 40 Code of Federal OreStudy Director =~ / Date: 3 203 IVMnaganEdagoefmveGnetn:daeetWanErctooMnSecl.ogy 11) oeI= ode: 130572002 Tar2r QUALITY ASSURANCE STATEMENT NOTOX Project 334351 NOTOX B.V.,'s-Hertogenbosch, The Netherlands "rTehsiusltrsepaocrcturwaatselayudreiftleedcbtythethreaNwOdaTtOa.X Quality Assurance Unit to ensure that the methods and "DTuhreindgattehseoofn-QsuiatleitiynsApsescutrioannsceprioncsepdeucrtieosnsapapnlidcaabuldeitos atrheisgtiyvpeenobfelsotwu.dy were inspected. DATES OF QAU INSPECTIONS/AUDITS onsite inspection(s) (Process) ONcotvoebmebre0r7-0157-,152,020020(2Ec(oAtnoalxyitcioclaolgyS)uppor) protocol inspections) (Study) December 06, 2001 report audits) (Study) April 29,2003 REPORTING DATES ONcotvoebmebre2r1,192,0022002 December 06, 2001 April 29,2003 Head of Qualty Assurance C.J. Michell B.Sc. Date: Ig, - 5-03 T4127 NOTOX Project 334351 SSoUwMM0ARRyY _ `Selenastrum capricomutum,96 Hour Frosh Water Algal Growth Inhibition Test wih T-4127. TPuhbeicsattuidoynprNooc.eLd3u8re3sPadretscCr-i3beaddoinpttehds Dreepcoermtbweerr,e1b9a9s2e;dOoEnCtDhegEuiEdCeliDniereNcot.iv2e0912,6A9d,opled June 7, 1984; and ISO Standard 8632, Firs edition, 15 November 1965. TT-e4i1p2h7en5ylasbeanlzycopnhsoissptihnognoifuP(ecraftuioonriocopcatra)n.esBuaisfeodnyoinmetthheyrleasmipdoens(eanoifontihcepaanrito)niacndpart of the t1e9st5s2u0b6stCan.ce water solubility of T-4127 was delormined to be betwen 0.6 and 0.5 maf at Tprheepasrteuddyasttaarltoeaddviintgharatmeiotf t1e0s0t megx1po,swihnigcehxpwoansenmtaiganleltyigcraolwlyintgiarlegdaefotrohareistedraeydssobleuftoiroen foitmrati1o.n2. mA gblatnok-bceolnotwrotlhewamstaolsfodientceucdteido.n dCuornicnegntthreateisotn.sTihnethbeiofliotgeirceadlsroelsuutlitosnsdheocwreedastehdat acopnpcreonxtirmaattieolny. total inhibition of cal growth and reduction of growth rate occured at the mit Aa WfiantalerteAstccwoamsmpoedraftoermdeFdreaxctpioosnin(gWAexFp)onpernetpiaarlleyd gatroawilnogadailnggareattso o4f5,10100,mg2/0l,a4n5daandbl1a0nk0% of cdiocnhtioolrofdoirmaetmnyasxiilamnuem. oAfl9th6ohuoguhrsv.apAlolugrlparsesswsaurreeuwsaesdrienlatthievefliynalotwe,stbawsaesdporne-tthroeaHteednrwyith - choenssttaundtyewvaaspopraetrifoonrmweadsistialira-tipgohstsviebslseelcsa.usSeamfporldeesctraekaesne doufrtiensgt tchoencfeinnatlratetsitonwse.rTehaedrdeefdo!re itecty 10 isopropanol ata ratio of 1:5 0 prevent any additonal oss. fSrionmceth1s00le%veelffweecrtewaansarleyczoer.deRdesiunltthseslhoowwesetdttrheaatttmheentmteharsouurgehdouatntihoen tceostncoennliyrsaatmopnlveasriteadken between 77 and 87%, raliive to the infial concentration. tInemtpheercaotnutrroelcso,ncdeiltlidoennssirteymaiinncreedaswietdhibnytahne aravnegreasgeprfeacstcorriobfed>b1y6twhietphirnot3ocdaly.s.HoFwuervtehrer,,pH Vienscsreelass.edHebnycem,orteisthdaenvi1a.t5iounnidtdnntohteabffaenckt-tchoenlvraol,idbyutcrtihtiesrwioansforrealigaatletgodrotwhteh.air tight capped Tc-a4p1r2i7coimnudtuucmedattoctoanlciennhtirbiattiioonnosf <ce0l.l 0g5romwatlh.aHnedncteot,althreed9u6ct1i-oEnCsof. gorfohwtsh frraetsshowfaSteelreaniagsatsrum Species exposed 0 T-4127 was < 1 mg (< 0.05 mg). Leinmsiutriendg.thTehearnaaliystiacarleasluisptpiocrptososniboinlltyytthheatatnhieonaicctpuaarltcoofncTe-n4t1r2a7t,iovnasliodflythoef ctahteiotneisc cpaorutldwenrotebo fhuirgthheerr arnesdemaorcrheasntdabldeectihdaondt0osasccofepthtehaenhiiognhilcyptaorxti.cHloawbeevleinrg,(tRh5e0s)pboanssoerd iondtnhoetcagurneetwilh resus. T4127 NOTOX Project 334351 PREFACE Sponsor Study Coordinator Study Monitor Testing Facilty ASqtuuadtyicDiTroexcitcoorlogy: `Technical Coordinator APnrailnyctiipcaallScCiheenmtiissttry: Study Plan 33MM CCeonrtpeorr,atBeuiTlodxiincgo2l2o0gy-2602 P.O. Box 33220 ST. PAUL, USA. MINNESOTA 55133-3220 Mrs. M. Mitchell `M3rMs.EnOrv.irSo.nmBeenatcahl Technology and Safety Services 9ST3.5 PBAuUsLh,AvMeInNuNe,ESBuOiTldAin5g521-4345-09 USA. NOTOX BV. H52a3m1baDkDen'wsa-tHeorritnogge7nbosch `The Netherlands DMrrss.. MW..BKoogoelresn Ir. M..C. Brekelmans SSttaarrtt oPfrofjiercstt:exDpeocsuermeb:eJrul0y5,012,0021002 `Completion last exposure: November 15, 2002 ``CCoommpplleettiioonn Apnroajleycst:is:MaNyov1e3m,b2e0r0315, 2002 TEST SUBSTANCE DIedsecntriifpitciaotnion Batch Composition TSetsatbistuybsutnadnecressttoorargaegeconditions. Expiry cate Stabilty in water T4127 Dark amber waxy solid D'-952-49919%lotF2luoroslastomer curative. <<21%% NMe-tMheytlhyAllcPoehroflluorooctanesuiphonamide <19% Ethyl Alcohol <19% Isopropyl Alcohol ASttabrloeo.m temperature in the dark 31 March 2003 Notindicated The sponsor is responsible for al test substance data unless determined by NOTOX. T4127 NOTOX Project 334351 PURPOSE `gTrheeepnuarlpgoaseeinofa tshehosrttu-dtyerwmaesxpteoreivmeanltu.ate the test substance for is abilty to inhibit the growth of GUIDELINES `8T6h9e2:stWuadyteprroqcueadluitrye-s Fdreesschriwbaetdenr atlhgiaslrgerpoorwtthweirnheibbiatisoendteosnt twihtehISScOeInnetdemeastmiuonsalsuSbtsapnidcaartuds and Selenastrum capricomutur, First edition, 15 November 198. Ipnreasdcdritiiboend,btyhethperofocleldouwriengs wgueirdoelidneessi:gned to meet the test methods and valdiy criteria `+ dEeutreorpmeiannatEicooofnnoecmoitcoxiCcoimtym,uPnuibtlyic(aEtEioCn),NoE.ELC38D3i,reCc-t3i:ve*9A2lg/a6l9,InPhairbtitCio:n MTeetsht"odasdofportethde. December, 1992 `-OTersgtainnigzoaftCihoenmfiocraElsc,ongoumidiecliCnoe-oNpoe.r2a0t1i:on"AalngdaD,eGvreolwotphmeInnhtib(iOtiEoCnDT)e,stO",EACdDopgtueiddeJliunneefo7,r 1984. ARCHIVING NreOfTerOeXnceB.sVa.mwpillleaarnchdivreawthdeatfao.llNowoindgatdaatwailfobreatwilteahsdtra1w0nyweiatrhso:uptrtohtoecsolp,onrespoorrts,wtreistttesnubstance consent, DEFINITIONS ~~ GCorlolwdtehnssittyheisitnhcerenausmebienrcoefllcdeelnssipteyromveirltehe.test period. - Gthreowgtrhowrtahtecuisrvteheininaclroegaasreitihnmciecllpdite.nsiFtoyllpoewrinugniftrtomme.theItmisatdheermiavetdicfarlomnatthueresloofpe under `EexxpCouneinstitahlegcroonwctehn,trtahteiomneaofsuterset osfubtshetasnpceeciftihactgsruowltthsrainteai5s 0pr%efreerdaubclteioonveirn gbrioowmtahssr.atTehe relative to the control. ~ Tisotdaelrigvreodwtfhroomrtbhieomaraesasuisnddeerfitnheedgarsowttheh icnucrrveeasineanlitonteaalrepllolt.dTenhseitEyxoCviegrsthtehetest period. It ctohnecceonnttrroalt.ionoftest substance that results in a 50% inhibition of total cell growth relative to - mNeoaOsbusreerdvpeadraEfmfeetcetr(Cso)ncsehnotwr(ast)ionno(sNtaOtEisCt)icailslythseighniifgichatntteefsfteecdt oconnaclegnatlragtrioownthatrwelhaitcihvetthoe control values. ". 9fo kt hoRS Tar27 NOTOX Project 334351 TEST SYSTEM Species Reason for selection Control of sensitivity `Selenastrum capricomutum, strain: NIVA CHL 1. "This system is an unicellular algal species sensitive to toxic substances in the aquatic ecosystem and has. boon selected as an intemationally accepted species. "The resus of the most recent roference test with potassium dichromate (Merck, Art. 4864) are `appended to this report. FRESH WATER ALGAE CULTURE Stock culture Stock culture medium Pre-cuture Pre-culture medium Algae stock cultures were started by inoculating gagraorw.thThmeedsiusupmenwistihonaslgawlercoslcsonftrionmuoaupsluyreaecurlattuerde aonnd exposed o light (4000-9000 lux) n a climate room at a temperature of 23 + 2C. Mi; formulated using Mill-RO water and with the following composition: nano; 20 mot KMSPOOL,THO nmoo NaiCO, 1040 sng KCHHAOO,HO 5 mo mor Cac HO CFOs HO mot 5 ma HWeno, 2To9r mmoott 2C0u,soLsH0 ort 008 mg mg (HIM 0s 84:0 oot mg 30r 4 days before the start of the test, Cells from the algal stock culture were inoculated in culture medium maatianctealilndeednsuintdyeorft2h.e1s0*amceellcs/omnld.itTihonesparse-ucsueldurien wtahse. tbeesfto.rTehuesec.ell densitywas measured immediately iMn2h;ibiatcicoonrtdeisntg" Ntoovt.he1198S9O;-Sftoarnmdualratde*dAlugsailnggrMoiwltlh-Q cwoamtpeorspirteivoenn:ting precipitation and with the following NHC 5 mg cMaciL2H2O0 2 mmoo MKaPSOO,LTHO 51s mmogt NFaeEChOaTHAOZHO wLI ae 7. T4127 NOTOX Project 334351 #W80n,4HO 2Cov4i sn0 rae NNaaHMCoOO,LHO Harness Canig) awsee sw 0150 uot Te ww024 nmemtol 24mg Cac) 83:02 PREPARATION OF TEST SOLUTIONS "dTihsesosltvaenddtaersdt tseustbsptraoncceeducroenscerneiqruaitrieodnsgeonfesrtaatbiloenaonfdtehstomsoolguetinoenos,uswhmiicxhtucroenstoarindeidspceormspilonest.ely Tpohsesitbelseti(ngo.o.f fciomncoefnttrhaettieosntsstuhbasttwaoncledodnistthuerbwattheertossutrtsacyos)t.em were prevented as much as TTr-i4p1h2e7niyslabesnazltylcpohnsoisspthionngioufmP(ecraftliounoircoopacrtta)n.eBsausifeodnyolnmetthheyrleasmpidoens(eanoifotnihce apanriio)naicndpart of the t1e8s.t5s2ub0s.t6anCc(eNwOatTeOrXsoPrloujbeictlto3yf40T8-4421,2u7swinagstdheetceorlmiunmendetloutbieonbmeettwheoedn).0.6 and 0.9 mg at LAi'msittoctkesstolution was prepared by weighing 100 mg of T-4127 on a glass surface. After placing mthge.caTrrhieermiinxtaumreeawsausritnhgenflmaasgkn1etliicraelolfytsetsitrmreeddoirumthweaesdaaydsd.edAfttoerotblhaeisniarilnogadpienrgiordatteheo.f 100 fmliaxstk.urTehweamsixctleuarre bwuatscfonitraeidnetdhtreosutgshuabsptaapnecrefpiatretric(lSecsh,lewihcihcehrsaentdtleSdchautetlhle6b0o4t)ttoomroefmtohvee the lcoalroguerrleusnsd.isNsootlevetdhattestthesubblsatnak-nccoentpraorltircelecsei(vcae.d >th5ems)a.meTnterefaitnmaelntte.stAfstoelrutpiroenpawraasticolne,arvaonludmes aofde5q0umaitewevroeluamdedseodftaoneaalcghalrespulsipceatnesioofnthweerreespaedcdteidvettoeestacchonrceepnltircaattieonp.roSvuibdsinegqauceenltllyd,ensity of 10+ cali. FAilnaglltaessstware used in the fina test was pre-treated with dichlorodimethyisiane to prevent loss of t2e0s0t smugbsotfaTn-c4e1d2u7eotnoaadgsloarspstsiuornfatcoeg. aAfsersuprlfaacceisn.g tAhestcoacrkresroliuntiaonmewaassurpirnegpafrlaesdkb2ylwiereigohfiensgt mSoeldutiiuonmwwaasstardadnsefdertroeodbttoaiansaeplaoraadliinognrfaufnnoefl1a0n0dmlegft.0Asftaarbitihseesftorri4nghopuerrsi.odS,utbhseeqstuoecnktly the WLaotweerr AtecsctocmonmcoednatrtaetdioFnrsacwteiroen (prWeApFa)rwedasbysleupatriaotneodfftrhoemWtAhFe.cenAtlrefoinfal ttheestsseoplaurtaitoinosnwfeurnneel. cvloelaurmaensdocfo8lo0urmlieswse.reNoatdedtehdattotheeabclhanrke-pcloinctartoelofretcheeivreesdptehcetisveamteesttrceoantcmeennttr.atAifotnertporrepeadruacteion, tihnestheeadadosfp5a0cemgas)mtuocphreavsenptosdseipblleet.ioTnhoefaClOga.l mSeudbsieuqmuecnotnltya,inaeddeqexutartaeNvaoHlCumOe;s(o1f5a0nmaollgal suspension were added to each replicate providing a cell densityof 104 celisiml "Re 000068 T4127 NOTOX Project 334351 LMT TEST Aaltiamiltoatdeistngwarastepeofrf1o0r0memdg!exapnosdiangbleaxnpko-nceonntiiraolllyorroawpienrgioadlogfas72ohaoufrse.red solution prepared `Sampling:VForleuqmueency Storage a1t0tm=iohf,ro1m=2t4hehaapnpdro7x2imha.te contra of the test vessel. oNfotsaamppplliincgab.le, all samples were analysed on the day FINAL TEST. TEST CONGENTRATIONS Ta127 Controls Repicates 4lo5a,di1n0g,2ra0t,e4o5f a10n0d m1g00.% of a WAF prepared at a T(oBsatnkmeecdoniturmo)without test substance or other addilves 36 rreepplliiccaatteess ooff ethaechbatensktccoonntcreonlt.ration. c1onrterpolilcfaotresoafmepalcihngtepsutrcpoonscese.ntration and the blank2alrgeapsi,cfaitleesd oof tthhee rhiimghaensdt tpersotteccotnecdenftrroamtiloinghwtifhorouthte course of the test TEST PROCEDURE AND CONDITIONS Testiype static Test vassals A1t0h0omulg,halvl-agplaosusr, pressure was relatively low, based ocnautshee fHo endreycrceonassieaonft teevsatpcoornacteinotnawtiaosnss.tlTaheproesfsoirbele he study was performed in air-tight vessels. Medium M2-medium Goll density Anintial cel density of 1 x 104 celisimi Test duration 96 hours mination Cofon3t0inWuaotutslwyituhsiangigThLt Din-taenmspitsy owfitthhien ttyhpee r`aCnogoel-owfh7il3e 0102 pEms" Incubation sDuusrpinegnsiinocnubbayticoonntthienuaolugsaschealklisnwge.re kept in Ca re T4127 NOTOX Project 334351 `SAMPLING FOR ANALYSIS OF TEST CONCENTRATIONS rDuopmliscaetpearsaatmepvleesssawlesrsofttahkeen4.f5r%omdailtliiouniaonndstahnedbltahnek-bcloanntkr-oclonattreorl 4di8reactnldy9a6fehrouprrso.paTrhaoton, method of analysis is described in the appended Analytical Report. Sampling:TrVeoaltummeent storage `sSmaim.ples wero added directly to isopropanol at a ratio Nofot1:5 app0lipcrabelvee,ntsaamnpylaedsdtwieornealansasl.ysed on the day of sampling. MEASUREMENTS oH Temperature of medium TAhttahpeHbeogfitnhneinsgakatnidonas sthheounlddporeftehreabtelsytnot deviate by more than 1.5 units during the test. Gontinuously in a temperature-control vessel. RECORDING OF CELL DENSITIES 7TAth2te0hrneeambteaugrsiincnnagilandgeVonafsriittahineesCteawsro,yrce5al0dlesstiwenerglrmoienbceodeuabnytessdppeebccytrromoippchhroootlsoocmmoeepttera,ircuwsimitenhagisamumcroeeurmnsetiniotnngopcfrhsoaabmmebpolre.s at a(psabtlhalnekn.gth =20 mm). Varian Nederland BV., Houten, The Netherlands. Algal medium was used DATA HANDLING eee Calibration curve: SeQxuutasinpntecitnfisiocinaotunisowinnioghfsdcpiaeflceirdreeonnptshiaotllelosmdewelnarssiitcibemase.saeTsdhuoronecmaaelcinabtlrsiabtrioafotnaicomnuircvnuerivmwe.uamsCaoclfolsmdipxeonlssuiettdyiouwnsasisnogfpllaainnteeaaldrgvalersus veagrrieosussiotne.stTsholeuteiqounastiatondioffetrheinstcpuorivnetswainsitmheencuursiendgothecatlecsutlpateeritohde cell densities of the `Comparison of areas under the growth curves: The area below the growth curve was calculated using the formula: anticte Mooeo2utlyg Mo tl. eoBilsy, Where: AR==anroemainal number of calls/mi at the start of the test NNii=e mmeeaassuurreedd nnuummbbeerrooff ccaallissmmli aatttt, =2 itimmee ooff ifi"rstmmeeaassuurreemmeennttastaefrtbregbiengniinnnginogf otfhethteesttest vide . iy 2A 0100 4 " tht Limittest: Table 1: Mean cell densities (x10" cells/mi) during the limit test opmen [o aa Taw T T 7 | [Benkcowo [70 ss | sao [ 12s | Fone somsgtg-------- [Crooimeres[0Tvs 1 37 | 17 Loading rate | Cell growth (6-72 rW e) id gn) [Meanarea (&) inhibition(3) (0-72hrs) Reduction (| [elankconrol| 2esa16| [ ooeras| | (Crooifiterss| 6536 | 965 |ooor2t | s93 | (mg [osT s | [BlankconelT5000] CrooTi "8t 0 i1e5e0d] (00mgn) [s1a ]Sam mplep2| mleane| [es Toon|oosse |ooewr | [ Tom0 e[amo |otse | [ss Tose|ore|oram | T4127 NOTOX Project 334351 Table 5: Mean concentration in the lowest treatment duringth test T4127 | ConcentrTa41t2i7 on (100 mg [4s Toons|oours|oosss| oTfaTb-l4e1267s.hoTwhsemreesapneccteilvledgernoswitihescumrevaessuarreedsahto2w4n-hionuFriignutreerv1al(sssate tthhee dAipfpeernedntixco|nfcosrntihreatcielolns densities per replicate). Table 6: Mean cell densities (x 10 cells/mi) during the final test "soTfaaiWzaF Exposure time (hours) aoomgn) | oT 2Tas |wes| [oankconvol T7| 0 7a |4 sss |5 eer | [esTwo To[wo [wo | 10 | [oo ToThe 0[v0 | 10 | 20 "ToT001 |0 10 | es ToTo190 | 10 | 10 | Choo Te TT v0 | 10 |__10 J Experimental conditions Tofabelxter7a sNhaoHwsCtOh03e ptHherMec2o-rmdeeddiautmthpeHbeignicnrneiansgedanmdortehetheannd1o.f5tuhneittsesti.ntIhnespbiltaeniocfctohnetraodldidtuiroinng hameotuenstt poefrNioad.HCTOhissnwatsheresloaltuetdiotnostahnedatrhteiglhitmliytecdapCpOe;devxecshsaenlsgeasfraomretsuhlethoefatdhsepalicmei.ted "pTehreiotdemtpheertaetmupreeraotfutrhee mteesatsmuerdeiduimn twhaesi2n3c.ub1atCaortwhaes mstaairntotaintheed tbeestt.wDeuernin2g2.t6heanedxp2o3s.u6rCe. Table 7: pH levels recorded uring thefinalest. ofa WAR (oomgn | 0Tos| [Moa|nk84c| owto oa l| [asTa | s as | [o a4 |T 83 | [eo7a TT 82 | [Tes 1 es 1 81 | [ 7TC 786 |0 s2 | "13. P T4127 NOTOX Project 334351 ACCEPTABILITY OF THE TEST 1. In the controls, cell density increased by an average factor of > 16 within 3 days. 2. Temperature conditions remained within the ranges prescribed by the protocol. However, pH increasedbymore than 1.5 unit in the blank-conirol, but this was related to the air tightly `capped vessels. Hence, this deviation did not affect the validity criterion for algal growth. 3. Analysisof samples taken from the lowest treatment showed that the measured anion `concentration varied between 77 and 87%, relative to the initial concentration. However, this concentration remained far more stable than similar concentrations in the fish and daphnia. tests (NOTOX Projects 334338 and 334349). This was probably due to the difference in `composition of test medium. CONCLUSION Under the conditions of the present study with Selenastrum capricomutum, T-4127 induced total inhibition of cell growth and total reduction of growth rato of this fresh water algae species at concentrations < 0.05 mg. Hence, the 96h-ECso of Selenastrum capricomutum exposed to T-4127 was < 1 mg (< 0.05 mg/). Limiting the analytical support on only the anionic part of T-4127, validity of the test could not be. `ensured. There is a realistic possibilty that the actual concentrations of the cationic part were higher and more stable than those of the anionic part. However, the sponsor did not agree with further research and decided to accept the highly toxic labelling (R50) based on the current result. T4127 Figure 1: Growth curves at different concentaotfTi4o12n7s, 5 70 | E 60 <8 50 x340 Zsz 30 20 |3 ox 0 . 24 48 72 Exposure time (hours) NOTOX Project 334351 Tar %10o 0amWaf AnF | mw pected --aois at | x20 nts --e--100 96 Ta127 NOTOX Project 334351 sREe FEReENCrE TsEST 0000 (`SNeOleTnOaXstPrruomjcecatpr3i5c65o4m7u)t.um, fresh water algal growth inhibition test with potassium dichromate CStoamrptloeftifiornst leaxsptoesxuproes:urSee:ptOecmtbobeerr3003,,22000022 JPTuuhbnelecsa7tt,ui1do9yn8p4Nr;oo.caenLdd3u8rIe3SsOPdaSrettsacCnr-di3abreadddoi8np6t9th2ei,ds FDrieerpcsotermtedbwieteirro,ne,1b91a95s2e;NdoOvoEenCmtbDheergEui1Ed9Ce8l9iD.niereNcot.iv2e0912,/6A9d,opted TPhOiTsArSefSeIrUenMceDtIesCtHwRaOsMcAaTrrEie(dMoeurtckt,o cAhre.c4k86t4he, sBeantscithivKi2ty89o7f4t7h6e4t)e,st system used by NOTOX to A1l8g:aaendwe3r.e2 emxgposaendd ftaoor abplearnki-ocdonoifro7l2. hTohuresitnoitaKl.Ccerl.l0d,encsointcyenwtarsat1i.o0nxs o1f00.c1e8,ll0s.i3m2, 0.56, 1.0, Resuts: Calculation of% reduction in growth rate in the reference test: Concentration KOO; | MeaTnotAarleacell growth: Inhibition| inteGrvraolwth raeR:eduction (mg) foro72n * 0720 % [ F oo ttTTT emaeose]Tee | oom| oor| oi [Fooss eT TT reo ssos || 1as 5a2| | ooooc ssseeses | |o 3ys3]| Fro TT Fe TT mes| ase ae | oom we| oowms | ur] ame | Ce wal ws| eos| sme] 0dU.in5cd6herrmogmta!hteaecnoidnndhhiiitbgiihtoeendrscaoefnlldtghrereordweutfcheoerfdetnghcrieoswsitfhruedrsyahtwweiatalhtteS1re.a0llemgngaae/stsarpnuedmcihceiasgpharetri.cnoommuitnuaml,copnocteanstsriautmions of brTaehntegwieEneCgnsf0rf.oo4mr9c0ea.ln7l8dg1r10o.4w1t.mh6gmi.gn/hilHb.eitTnichoene,(hEits4hteCoarEi:cCa0sl-:7r2a00n)-g7ews2ahosffo1tr.h1tehm7eg2phfrewEsietCnhtafbo9art5%gchrocwcotorhnrfieinsdhpieobninctdeisoinwniltieterhvatlhis range. 2rTanhndegi2En:Cg3ymf,r1foo.mr g1Hr.e6onwc0tah,2.rt2ahtmeegrEex.dCuiTccht:ieo0n-hi7s(2tE1oxrCfieocsar:lth0re-a7np2gr1ee)ssewfnoatrsgbr1a.ot9wchtmhacorfartwreietsrhepdoaunc9dt5siow%niltcihotnhbfieistdrweaenncegene.i0n.t6e2rval dTehsecrpirboteodcoalb,orvaewwdaastapearnfdorrempeodrtunofdetrhisGLstPudcyonadrietikoonpstwiintthhae QNAO-TcOheXckarchives. The test APPENDIX| araxm------ 00mg) [0 [72 Tw [ 7 | es | ANALYTICAL REPORT 96 HOUR FRESH WATER ALGAL GROWTH INHIBITION TEST WITH T4127; DETERMINATION OF THE CONCENTRATIONS NOTOX Project 334351 NOTOX Substance 113607 -Page 10f20- Ta1z7 REPORT APPROVAL PRINCIPAL SCIENTIST: NOTOX Project 334351 yn (IAnaMiyiCca. BrCohkeemlimsatnys) ose: SagMay 320, 08 T4127 NOTOX Project 334351 ePeRgEFAACCEE Analytical study SCtoamrptleted: 0115 JNuolvye2m0b0e2r 2002 Be PRURPOOSE r ------ ee-- a`nTahleyptiucraplomseetohfotdheusaenda.lytical study was to determine the test concentrations and to validate the pRerEcAeGNEwNyTrSs Mill-Q water M2-medium Methanol Isopropanl (2-propanol) Ammonium acetate 2.0 mM ammonium acetate. Internal standard solution Isopropanol containing internal standard sTuabpsweaquteenrtpluyripfiaesdsebdy orevveerrascetdivoastmeodsciasrabonndand ion`exchange cartridges; Millipore, Bedford, MA, USA see main report HPLC-grade, Labscan, Dublin, Ireland p.a., Merck, Darmstadt, Germany. Factopur, Merck, Darmstadt, Germany 154 mg ammonium acetate in 1000 mi Mili-Q water s5u0lf4onoatfae isnolmuetitohnaonfl1f2i0l4edmugp/ltope5rfmliuwriotohc5t0a/n5e0 (vv) M2-medium/methanol, m1e4t0hualnoofl2f8il1e6d mupg!top2e0rf0l0uomrlowoictthaniesospurlofpoannaotle.in BIINTEERRNNALASTTAINDEARDDeen NIdaenmteification number DeBsactrcihpntuiomnber PuErxipitryy Date CeStrotrfageedconditions Supplier "PAeSrSf1lu7orooctane sulfonate (FC-95) WLhoitl2e17solid (determined at NOTOX) 20.49% Y21esFebruary 2003 (determined at NOTOX) AMtmroom temperature in the dark `The sponsor is responsible for al test substance data unless determined by NOTOX. T4127 NOTOX Project 334351 SSAMAPLME PPRELTREEATPMERNTEPRTOCERDUEREATMENTPROCEDURE Analyses in July 2002 {Ehaeicnfhtlseaasmkamslpwlseteraen(1df0airmldeid)swoulpausttiooqtnuhawenatmistaaratkdivdueesldiynttgoramtnehstefhesaranrmeodplltieonstaet2ran0kaelmnisatvtaon2l4du,am4ertd8raciocnndfclea7nskt2.raAhvotouirolsn,u1ma2fe0eoryfgw2)h0.i0ch No internal stanwadsaadrdedd 0 the flasks containing the =0 samples. Analyses in November 2002 arIdandnoistrfiedorneratlerodipntrofeoavrem5an0ttimfolrnovsmoulpaudpmsloeirteprdliicboynl,atshkie.t swApaosvnosdloeurcr,indeeoeafdcht2os5camhmiapnligseoep(5sroampmiap)nlowelapscroeqn-uttaanriteniaittnamgteinivtne.tleyBrnaasled on standard was added 10 the 50 mi-fasks (intemal standard concentration 164 yg) AARNAALYTTCIACALLMMEEMTOHOD 0000000000000 wTTer4ir1pe2hoe7nnlyyibabaesnsazletydicpoonhnsoitsshptehioannngoiioufnmiPc(ecrpalaturiotonroiofcoTcpat4rat1)n.2e7sUupulsofionnngryeiHqmiuegethshtyPleoafrmftiohdreemsa(pnaoncniesoonLrii,cquqpiuadarntCt)hiartonatmdiavteoagnraalpymsye.s. `wnTiohttehrseeMfnaosrsiets,ivSaepesecentcorouongmdhetmtreoitcmhedoeadtsewucartsieocnuos(necLdeCnMitSnrMaNSto)ivo.enmsTbihenerpra2en0at0lr2ye.taitceadl smaemtphloedsufsreodmitnheJufliyl20t0ea3t.wae Analytical conditions for the analyses in July 2002 Column Mobile phase Flow CAuotlousmanmptleemrpetreamtpureer:ature DeItnjeeccttiioonnvolume T4127, anionic part Internal standard BKeaylsatsonCe1)8, 50 x 20 mm; d,y=m (Thermo Hypersi GErlaudeinesnAt: 2.0 mM ammonium acetate Eluens B: 100 % methanol [[T0i0me0(min)"TT-- EeiouensT A(3%)|H Eiuens0 &(%|)] [Ho [so Teo To TT s os] EI -- -- [too [300 Teo Too "Te1 o 0] `3a0m0bileinmtitnemperature. `1a0m0biuetnt temperature. JLoCsQe,DCuAo, mUaSsAs)spectrometer (Thermo Finnigan, San EPoSsitnieognat3ive mass-mass detection CIsoolliastiioonn weindetrh:gy:1.350% EMSzI neg5at1i2ve0m-a3s8s8d2e,tec4t1i0on0 PMozsition43992 T4127 NOTOX Project 334351 Analytical conditions for the analyses in November 2002 Column Mobile phase Flow ACuotlousmanmptleemrpetreamtpuerreature DeItnejeccttiioonnvolume Interface Monitored masses KBeeytsatsonCe1)8, 50 x 2.0 mm; d,= 5 ym (Thermo Hypersil GErlaudieennAst:2.0 mM ammonium acetate Eluens 8: 100 % methanol [T[iomoeo(m--in)TTeEovensA("%)T|iEvoens&(3%) Hoo [380 TeT o io] 15 Te ---- [p 800 s TT os o h To e ] L31000l0imi"Te9 o 0] 25C aoc (S1AC0pIp4lEiXedMBSiMosSysstyesmtse,mTAorPoIn-t3o0,0Cmaansasdas)pectrometer oTpuerrbaoteiodnisnprnaeygaatti4ve50ioCn;mNoydfelowrateof7000 mimin. MMRRMM itnetsetrsnaulbssttaanncdearmdzmi5z124.919.=0>-->1689.9.80 Standard solutions. `Standard solutions of T4127 were prepared in methanol Calibration solutions for the analyses in July 2002 `Ouepnxcfeerpaotcmhfotrdwasoyasmotpfalnaednsaalrtydaskissoel,nutcaiatolnitsb=.r0atIhionoutnresrs.noallIunstaitdoavnnesdratiernndStlw0ya5,s0nao(dvi/dvne)tdemmetatolhasatfniaonnaldlMac2rod-nmcweeandstiruaamdtidoewndeorfe01mt2aa0dsgeel samples. Calibration solutions for the analyses in November 2002 OiSonnltuetrtinhoaenlsd.satyanodfaarndal(yisnitse,maclaisitbarnadtaorndsoclountcieonntsraitni1o/n51(6v4vu) gM2)-wmeedrieummiaidsoepurpopfarnoamitwcoonsttaainnicnygd ~VALAIDDATIAONTOFOTHNE AONAFLYTTICAHL EMETAHOND ALYTICALMETHOD wTahse LnoCtMcoSmMplSetmeedt.hoTdhseupsaerdamweetreersonvlaylipdaartteyd vaarleidgaitvede.n bUeploown.request of the sponsor, validation sdaenceicdteodn1voulseu1me0sofin2j0e,ct5i0onaonr a1n0a0lysiwseorfesatmepstleedsb.utaccuracyandraisonwasntmpovedand heels as T4127 NOTOX Project 334351 Validationofthe analytical method used in July 2002 `Specificity scBrluitabincskaellMqy2ue-evmnaeltduiaitnujemed'cftowedaiisnntpierprelfteirrceianatgteepdienaatoskstshpbeeycHicfPoiLmepdCairsnyi`ssstoaenmm.pwlietThhpecrhertreroseumalatttimnoeggntrcahpmrrsoocmoeadftauorgfeer'satamsnsudbwsetraence solution in the same medium. Interfering peaks are required (0 be 5 30%ofthe LOG, Linearity sFmteratondhmatarnwdo.olEsMta2acnhmdeaodrfidtuhsmeo.sleuTtshooinlssutr(ie1os0nus2lt0weadasnidnin21j0ecc6ot0necdmeaniftn)rr,aptisicioganhtrtea.lnuRgteeiosofpnosnw0seer.se0wper50re.ep16ap90rl8oetdimeigdn "a5g0a/ii5nnt0set(mvailvh)e croenscpeonntsreatsiaonnsd.cAonlciennetarrarteigorness.sTiohnepcrororgerlaatmiownacsoeufsfeicdietnotciaslrceulqautieretdhetorabgaraetsslieoanstl0in.e09f,rom the `Staobfsiolluttioyns Srrioeplslupitcoianotsnees)"osvowefar0s.a0c8a5.i19c0,ulm8a.gt4e,dan0fdo.r150e.2a4c-mhhoocluornactneidnmte0r.pa9etr9ii6oondm,gr!esTpe4c1ti2v7elyw.erTeheinjmeacxteidmtuwmo dteivmieasti(oinnofthe Limit of detection (LOD) rnio0pil,si0eca5tl1ee.vmelIo9n fesatohclehutsciyhosrnotoefmmatth(oebgotrteahsmtis,nutmbVhs)et.taeTnstcheesuiLbnOsStD0a/nw5ca0es(pvceAaa)lkcmuheletaihtgeahdntaflwr/aosmM2tm-heemaemsdeuirauenmd'paewsaakwsenlielnijgaeshtcttheaedndin the mean noise level. Validation of the analytical method used in November 2002 `Specificity tBsiulcbaasneklqlMuy2een-vtmalelyduaiintujemedc'tfweodrasiinntpierprelfteirrceianatgteepdnetaaoskstshpbeeycHicfPoiLmedpCaisrnyi`ssstoaenmm.pwlieTthhpecrehrtreroseumalatttimnoeggntrcahpmrrsoocmoeafdtauorgteer'satamnSsudbwsetarnce. solution in the same medium. Interfering peaks are required (0 bo 5 30%ofthe LOG. Linearity rFerdomumtiwsoosptranodpaarndlsoclounttiaoinnsi(ng78i2ntaenmdal87s4tamndoa)r,d. sTihxidsirteisounlstewd einrapcroenpcaerneidraitnio1n/5r(avnigv)e Mo2ff0pel.go0tr0tee5sd0si1aog-na0i.in0ns8et9ft6rheommgc/ot"nh.ceeEnrtaerscaphtoioonfnstseh.seAsaneldisnoceloaunrtcireoenngtsrraewtsaisosinosni.npjeTrcohtgeerdcaoimnrrdweualpalstiicsoanetecd.Roeatofsipccaoiteneunslesatserweteqhureiered to be atleast 0.99. ,* iInntteerrnnaall ssttaannddaarrdd wwaass aaddddeedd ttoo aaffiinnaall ccoonncceennttrraattiioonno1f2016g4fgl. az DDAATTAAMHAANNDDLLING -VVAALLIIDATIONOOFTTHE ANALYTICALMETHOD Response: a TP[eakEorepa hke-- estmsaetsaanncnei "~ 13 NOTOX Proc ata w7hTerremsosreedot vsivee men Sanders svt: Cooficentcfvaraton: Maximum deviation: Unearty Validation of the analytical VeatnliGodiftah2e3ta0r8iatocanl method (November 2002) Recovery: Limiof eesion (LOD: JR il07 (n-1) (sancarddovition maan vee 100% VaM.E meat 3100 mh vt o [(highest - lowest)/mean] + 100%f he Night ard he lowost oSAfrCreigtyrswsaaosnrmetooaernmsawpdavasinnugsao0tcoa canlcitne theBrggrrroesioeonsting necessary a (1/concentration) weighting factor was used, Regression curve: Y =f X* + g X+ h Reyenseys=iao+nb hXCors:rceosproensmeon TT3o. mereepenion consans iT(Chhoenc3meinSttronaftidoe(tnpeecatanikaolnny'sledc)'a/ioCfnonveceeanetrrtaoteicosonnpcpernetpreaarteido)on +of1T0401%27 (SIN=3). ooo SE T4127 NOTOX Project 334351 Liofdmeteictitons (3 + noise level signa) conc. wnohiesree:level (N) heioghthte noise %] csoingcna.l (5) choneceinotgrahtthiteonteosfttseustbsstuabnscteanpceea(km[g%]) DDATAA HTANADLIMNGA- SANMPDLELANANLYSGIS-sAMPLEANALYSS Calibration Response: C(auilbyra2t0i0o2n) curve: BPoeakrareaa test sI ubstanceee Peak area internal standard Arcoengrcreeegnsrtserisaostniioocnnuspr.vreoRgefrgraromemswtshaiesonrauenssaepldoynsfsoiescswaaalncsdupleartfhoermed u(scionngcetnhterlaetaisotns)quweairgehstimnegthfoacdt.orffwnaesceussesd.aray RR == faeeCcierbgacon R:rceosnpcoenntsreatciaolnborfattieosnt ssoulbusttioannca in calibration 2.5.1.`s5o,luhtiorne[gmrge/sls]ion oeficients C(aNloivbreamtbieonr c2u0r0v2e): Aconnseiarcuhcldeadyousfinagnaeliygshits,coancceanitrraattiioonnscunrevcetoerdiinn was vilcate The coefficientof coneation was > 0.95 TtSeqhsuetasrrueebsssptmoaenntscheeo,wd)aussainncgdoann(e1el/aactroendrcewgeirnterhsasttihioeoncnao)nncwaeelniytgsrheattilineoganstof factor. R= asceb CR== croenscpeonntsroatciaolnibnratthioencsaoilburtaitoinon a = sSoilouptieoUnmmg]g] b = intercept Ousnedeafcorh qduaaynotfifacnaatloyns.isB,ottwhoccaallibbrraattoinonsscoultutoinonwsrweere cisunejrvevecentewsdaa(smipnlcdeousnp.sltirUcuasctitene)gdbtehfeorfeouarnrdesapftoenrseasm,aaxciamlubmraotfon Ld samples Recoroftocovrysamples: Concanvaton lavoronominal, CoQnuacdarnaatroonreasnsailoynseudy 202) Concentration analyse:d (Linear regression July 2002) Concentration analysed: (November2002) NOTOXProc334351 Concanaion snaysed [Ee p-- Concentration snaysea Concentation nomial CranSAR =g+yg?-dxfaihoi |, (Rb) +d C = ---- mon) a SRo ensgpon nsreegsaamspsloenucniosn]an "+s C = ------ [mgn] a 32R: esdspioooennsneaacsenargm]p units] 5: neonfont] T4127 NOTOX Project 334351 E-RLEVSIUALTSSL~DVALAIDTATIIONOONF TOHEFANTALHYTEICAAL NMEATHUODYTICALMETHOD rpTeahsrepaocmnaesltecesur.lsatTuihsoeinrnsegffootrrheet,hvesalovuamleeisdadatisifomfneertneetnsictoessnwemedirgiehntptbehreeftooarbbmlseeesdr.vuesdinwghneont-craolcuunldaetdincgotnhceensttraatitsitoincasland Validationofthe analytical method used in July 2002 `Specificity acFoningdtuaorifensa10n.ay9ni9n6dt2emrgsf/ehlroiTnwg-4cp1he2ra7okmssaoalttuottgihroena,mposrseisotpfieaocntboilvfaenltkyh.esoIftlewusttaissonucb(ls5et0aa/rn5ct0eha.(tvbv)anmketchharnoomlaMt2o-gmreadmisudmi)d not Linearity AcuarvueadirsasthiorwengriensFsiigounrwea3.s performed. The results are summarized in Table 1. The regression `Table 1 Quadratic regression curve. Conc{enmtoration Resupnoins]se 00.00871000 00..110929 00.540020 00..890%0 00..00115223/7 00.00111474// 00..00114230 00..00421163// 00.002411487/ 00..00232941 00..1008806411 00.01707868// 00..10170704 00..11362357/7 00..1135353/300..11348646 * Trpleate messursments. (iFnrto=hme6c.to0hn7ec6see6n9trr1esa0utlit:os0,n.tr2w2a0as59oc8fnoX0n.c.0lg0.u5d01ee60d--51th40a8.t"9t9Xh6;ermRe*gis=at0h.q9uo9aud7gr3aa)htbidceetrwevleaeitniaoorntesfhsmiipopoornnesetahnadn c1o0n%coenftfraetion calibration points from the calculated line was observed at some concentrations. Taz `Stoafbtheisolluttioyns The resuts are summarised n Table 2. Table2 Stabilty of 20.0510 mg T-4127 solution. Eiapsed time hours] Response 0002 0000111273 0854 0000112103 8897 0000111114 NOTOX Project 334351 Wiaximum devialion [3] Table 3 Stabilty of 0.102 mg T-4127 solution. Eapsed me [hours] Response 0002 00002211s3 8005 00224 00215 8824 0000221070 Wiimum deviation 2%} Table 4 Stabilty of a 0.996 mg/l T-4127 solution. Eiahposuerds]ime Response 0002 00..11653573 0495 001.611478 5s2a 00.11564725 Wiasimum deviation tl Fovreormatthleeassetra8e9su-sh,oiut rwatsimceonnclleurdveadattahactonthceenstorlautoinosn wofar0e.0s5t1ab0lme g(,maaxtilmeuasmtdaev8i4a-thioonur<2i0m%e) 0in1te0r9va9l5amt oacloncentration of 0.102 mg and at leasta 54-hour tm Interval ata concentration T4127 NOTOX Project 334351 iit of defection (LOD) F(iNr.)otwmhaetshmrdeeeeatncerhhmreioinmgehadttootogfrbtahemes1t1eo.s8tf%2s.u0b.Fs0rt5oa1mn0ctmehepgesaaskom,leuwtacihosnrodofemtatethroemgitrenasemtdss,tuobtsbhteeatn1ec0set0,s%tuhbUesstmiaenngacnethSneiosgiensael l(eSv)e,l vo1fa0ld0ueetysie.,ctBtiehoecnalfiuomsriteotfhseadsmeeptlseceatsmipoflnreo(smSi/tsNh0=e.3e)0c3ow6la2osxmiclca.ollcougliactaedsttoudbyewer0.e01c81itmegd batyaanfancetcotrioofn2,votlheummeitof Validationofthe analytical method used in November 2002 `Specificity FcBolinagtnuakriecnsihn4rgoaminnadfteorngasrlhasomtwsancddihdarrondom)taatcnoodngtroaafimnasa0on.fy1a0i0nbtlmeagrn/fklersTion4glu1pt2ieo7anks(sa1la/u5ttt(ohvne,v)proeMss2pt-emcotenidvioefulytm.h/iis(owepsatrsospcualbesnatoralntchea.t Linearity The results are summarized in Table 5. The regression ne is shown in Figure 6. Table 5 Linearity. Conce[n=tration Resunpiotnss]e 000000560919 000.181617/00,00796644 0000110%0 00122703//00.214282 0000595996 0188413/10171541 IntercSepitowpieth Y-axis WeightiRng factor 01021282 tconc0e9n9tr3ation * Dupicas mossuremens. Tl0i.hn0oe9aur9g6rhemldaugtpilioifncsaahti(ep1libcnejotencwcteeineotnnrsartseihsoopnw)owenesdiegahairniedlnagtciovfneaclecyntltoarrrasgteiuossenpdrn.eatdh,e tcwonacsenctornactiiounderdantghaetotfh0e.r0e0i8s0a1 osFnaolmrypqlpueasna.toitrattthieonionfesaarmipylreasn,gecatleisbtreadt.ionThceurcvaelsibwreatrieoncsonasltrouncstedaruesiinnjgecttweodbceofnocrenetarnatdiaofnesrovheer T4127 NOTOX Project 334351 B RESUe LTS -s SAMPu LE ANt ALYSs IS -sw0 memav 000e0 s `Samples July 2002 cTIanalaibdblrvaee6tristonehnostowlsnutotihoinenst.aenranlayltisctaalnrdaersduwsafsoratdhdeesdamtopltehsefsraommpltehse ptreesttrpeeartfeodromned01innJJuul0y 220a0n2d".the Table 6Concentrations of T-4127 in test medium (Full test 1), sTaimmpleionfg | sDaamtpelionfg | aDnaatleysoifs thous] | fodmmy]| ddmmyy)| [Nominal mon) | AnaIlmygse)d' || nRoemlianiavle[i%] : oua2| 02uk02 0100 n1d.200 n1a..209 100? 1537 11537 2 02ui02 | o2uu02 1000 n0g2.52 n0a2.52 100? 00624> | 00624 2 002 | od-uko2 110000% <<00003%622*|| <<00.00336622 + Mo<a2nofvcat analysis.Themaim devon ebenth reonsescaclatedfo eachsampleandwas 2Tw Coloaa ed by axvapiain. * iConncantrtyonnsobelfoewmatlhestatndfarddwtaesctaidnd.ed otesssamplessndcast soins nnad NNootdspopoiccaei. AobhantCleodvaailnug fwoecreaavteedoesusaing3nkherocuonndecdencaonscoonnr3atamna.roTnhesrdfonrsh,saamee:difernces igh be bsorved T4127 NOTOX Project 334351 `Samples November 2002 `Table 7 shows the analytical results for the samples from the test performed in November 2002. Table 7 Concentrations of T4127 in testmedium (Full test 2). sTaimmpeinofg|| sDaamptleionfg|| aDnaatlyesoifs | [hows] | [ddmm-| [dd-mm- Nommoiln]al | Analysed (mgi]| nRoemliantailve[t%o] wi wi 0 | 11Nov02| 11Nov02| 100 100 116529 0 11-Nov-02| 11-Nov-02| 0 nndd.. nnaa 00.00772211 0000657614 853 00..116600 00..116502 190%0 00332200 00332380 110023 00772211 00775442 110053 116600 116649 110053 48 | 13Nov02| 13Nov02| 00 nngd.. nnaa.. 00.00772211 00.00447717 6656 % | 15Nov02| 15Nov02| 00 nndd.. nnaa 0000772211 0000552463 773 na.MNooatndeotfecitpeidc.ateanaly. Themaximumdeviationbetweenth rosansescalculated foreachsamplewas < 0%, na. Not appicabie. T4127 `Samples July 2002 NOTOX Project 334351 000-2 ]1 Instotandard | 3i fanaar. SES 200,00.600.00) MS. .i Efoii | | i"i i i | EE wdws ni" i `Testsubstance Tio ae 4i15t0a 41eF9.c5E081 | 0 |2 |i 2 fui i wl RE -- STE -- TrainTT -- TTR Tg Figura 1 iHnPtLerCnachsvtaonmdaalrodg)ramof a blank (S050 (1) methanciM2-medium containing T4127 NOTOXProject 334351 -- -] Intomal standard wi io:r | 2[.|3 | | Lj i re esr. moe, . 03 || ol ee wise 2o1d resin ] S cse tod 1 Souris kL L>l3 be oT TTTTout ( Ii NL ---- TTT TH TR Figur2e CHPLoC cahroTmataogramof 0r.e368mg! T-4127in5010 vk)methanzmedium Taz Y=6.07669-005+0.220598"X-0T.4016257148X'2 016 0.14% NOTOX Project 334351 R"2=0.9973 W: Equal 5 = 0103 3 oo < o0o0l6 am 00233 o 000 rr 00 02 04a 06 08 10 mg Figure 3 Rinetgerrenaslsisotanndianred:forResoslpuotniosnessinag5a0i/n5s0t(cvo/nvc)enmiertahtaionnosl.IM2-medium containing Ta12 NOTOX Project 334351 `Samples November 2002 E BiE i Test substance E EiETi ---- | Lo {I [Ete i | Ea i Cddl |H i |{ ! i | il a | | | | i| i i| i ! re : Figur4e iHnPtLerCnaclhsrtoamnadtaorgdramof ablank (115 (vv) M2-mediumisapropanol containing Tarr NOTOX Projct334351 fgCE]H | Testoubeance | O i Hiii :i | :| i IYi | il || iHil LL : AE= E]I ores || S=NI i i .| i| | i i |[ i | i | | | Hi | ii: | | | i | _ : | Figure CLoCtMaiSiMnSg cmvoommatoagrrameof 0.100 mg T4127 in 15 (i) M2meduispropana T4127 4 | = NOTOX Project 334351 -- _ Figur6e Rinetgermeaslsistoannldianredf:orRseosluptoinosnessinag1a/i5ns(vtivc)onMc2e-ntmreadtiiounmsi.sopropanol containing