Document 6wzbnVnXgLk47vR419QopENYg

www.axysanalytical.com >)][( A X Y S Axys Analytical Services Ltd 2045 Mills Road West SIDNEY, BRITISH COLUMBIA, CANADA V8L 5X2 TEL 250-655-5800 FAX 250-655-5811 www.axysanalytical.com AXYS Client No.: Client Address: 4731 Weston Solutions Inc. 1400 Weston Way West Chester, PA, US, 19380 The AXYS contact for these data is Candice Navaroli. Page 1 of 234 www.axysanalytical.com PERFLUORINATED ORGANIC ANALYSIS TISSUE SAMPLES PROJECT NAME: Fall 2012 Tennessee River Sampling Contract: 4731 Data Package Identification: DPWG42640 Analysis WG42409 1 February 2013 Page 2 of 234 www.axysanalytical.com WESTON SOLUTIONS TISSUE SAMPLES PERFLUORINATED ORGANIC ANALYSIS AXYS METHOD: MLA-060 PROJECT NAME: Fall 2012 Tennessee River Sampling 4731: L19064-1 to -16 NARRATIVE 1 February 2013 This narrative describes the analysis of sixteen tissue samples for the determination of perfluorinated organic compounds using high performance liquid chromatography/tandem mass spectrometry (HPLC/MSMS). SAMPLE RECEIPT AND STORAGE The samples were received on the 8th of January 2013. Details of sample conditions on receipt are provided on the sample receiving records included with this data package. The samples were stored at -20C prior to extraction and analysis. SAMPLE PREPARATION AND ANALYSIS Sample extraction, instrumental analysis and analyte quantification procedures were in accordance with AXYS Method MLA-043: Analytical Procedure for the Analysis o f Perfluorinated Organic Compounds in Tissue Samples by LC-MS/MS. Approximately 2g (wet) of each sample was accurately weighed, spiked with labeled quantification standards and extracted in basic methanol. After extraction, the raw extract was gravimetrically split into two equal portions: a 1/2 portion for cleanup and a 1/2 portion for backup. The portion of extract for cleanup was spiked with labeled cleanup standards and then cleaned up using SPE cartridge and carbon. The extracts were then spiked with labelled recovery (internal) standards, and analyzed using liquid chromatography/mass spectrometry (LC-MS/MS). Sample MSF054 (AXYS ID: L19064-14) was analyzed in duplicate. The Duplicate was assigned the AXYS ID WG42409-103. CALCULATIONS Target analyte concentrations were determined using the isotope dilution/internal standard method, comparing the area of the quantification of the target analyte to that of the labeled quantification standard (surrogate) and correcting for response factors. Linear regression equations were determined from a multi point calibration series with 1/X2 weighting fit. Labeled analyte recovery was determined by using average response factors determined from the initial multipoint calibration and were used as general data quality indicators. Recovery values of the recovery (injection) standard were estimated by external standard quantification and used as indicators of the extent of signal suppression or enhancement. Sample specific detection limits (SDL) were determined from the analysis data by converting the area equivalent to 3x the chromatographic noise to a concentration in the same manner that peak areas are converted to concentrations. Formulae used for the conversion of the raw chromatogram data to concentration are provided in the method summary document, MSU-060. REPORTING CONVENTIONS For internal tracking, AXYS assigned Weston Solutions Inc. contract number 4731. AXYS logged the samples under unique laboratory identifier L19064-X; where X is a numeral. All data reports provided reference both the Axys ID and the client sample identifier. To assist in locating data, a table correlating Axys IDs with the client sample numbers is included with this data package. The report forms were generated using Laboratory Information Management Software (LIMS). Page 3 of 234 www.axysanalytical.com generated using Laboratory Information Management Software (LIMS). Any additional work performed after the initial instrumental analysis of the sample extract is indicated by a suffix added to the AXYS ID. The notation used to identify samples reported from extra work data or that were used as the duplicate of a client sample for this data package includes: (A) = the parent sample for a duplicate pair The following data qualifier flags were used in this data package: U = identifies a compound that was not detected V = surrogate recovery is not within method control limits The reporting limit (RL) was defined as the concentration equivalent to the lowest calibration standard analyzed in the initial calibration (CSO), corrected for sample size, or the sample specific detection limits, whichever was greater. Sample analyte concentrations are reported in units of nanograms per gram (ng/g) on a wet weight basis. Results are reported to three significant figures. QA/QC NOTES The laboratory procedural blank, OPR, and duplicate samples were prepared alongside the client samples, and were subjected to the same analytical procedures. The results were evaluated through comparison to the batch QC samples. Sample analyte concentrations are not blank corrected and should be evaluated with consideration of the laboratory procedural blank results. By virtue of the isotope dilution standard quantification procedures, data are recovery corrected for possible losses during extraction and clean-up. The Signal/Noise (S/N) ratios were measured as `0' for some compounds in the QC samples and sample data. This has been determined to be a limitation of the software and does not affect the data All linearity, calibration verification, OPR and labelled compound recovery specifications were met with the following exception: The recovery of 13C4-PFBA and /or 13C2-PFHxA in samples MSF126, MSF054, the Lab Blank and Duplicate (AXYS ID: L19064-11,-14, WG42409-101 and -103) did not meet the method criteria; these compounds are flagged with a `V'. As the isotope dilution method of quantification produces data that are recovery corrected, the slight variances from the method acceptance criteria are deemed not to affect the quantification of these analytes. Percent surrogate recoveries are used as general method performance indicator only. ANALYTICAL DISCUSSION No analytical difficulties were encountered for this analysis. Page 4 of 234 www.axysanalytical.com DATA PACKAGE This data package is assigned a unique identifier, DPWG42640, shown on the front page. The following documents are included in the data package: Method summary Sample Correlation Table Sample Receiving Documentation Laboratory extraction Worksheets Sample data reports (in order of Axys Sample ID) Laboratory QC data reports Instrumental QC data reports Laboratory QC and sample raw data Instrumental QC raw data Accreditation Scope I certify that this data package is in compliance with the terms and conditions of the contract, both technically and for completeness, except for the conditions detailed above. In addition, I certify that to the best of my knowledge and belief the data as reported are true and accurate. The following signature authorizes on behalf of Axys Analytical Services Ltd the release of the data contained in this data package. Signed: Kristina Coleman, Data Validation Chemist Date Signed m Page 5 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Summary of AXYS Method MLA-043 Rev 08 Ver 03: Analytical Procedure for the Analysis of Perfluorinated Organic Compounds in Tissue Samples by LC-MS/MS AXYS method MLA-043 describes the analysis of perfluorinated organic compounds (PFC) in tissue samples. Typical detection limits are in the range of 0.5 - 1 ng/g for 2 g samples. Target Analytes Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) EXTRACTION The default sample size is 2 g (wet weight). Surrogate standards are added and the sample is extracted by shaking with methanolic potassium hydroxide solution. COLUMN CHROMATOGRAPHY CLEANUP After centrifugation a suitable aliquot of the supernatant is diluted with water and cleaned up by solid phase extraction (SPE) using disposable cartridges containing a weak anion exchange sorbent. Optional cleanup may be performed with ultra pure carbon powder. The eluate is spiked with recovery standards and analyzed by LC-MS/MS. Calibration solutions are processed through the same SPE procedure. INSTRUMENTAL ANALYSIS Analysis of sample extracts for perfluorinated organics by LC-MS/MS is performed on a high performance liquid chromatograph coupled to a triple quadrupole mass spectrometer. The MS is run at unit mass resolution in the Multiple Reaction Monitoring (MRM) mode. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 1 of 6 Page 6 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Analytes, Ions, and Quantification References Target Analyte Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutane sulfonate (PFBS) Perfluorohexane sulphonate (PFHxS) Perfluorooctane sulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) Surrogate Standard 13C4-Perfluorobutanoic acid (13C4-PFBA) 13C2-Perfluorohexanoic acid (13C2-PFHxA) 13C2-Perfluorooctanoic acid (13C2-PFOA) 13C5-Perfluorononanoic acid (13C5-PFNA) 13C2-Perfluorodecanoic acid (13C2-PFDA) 13C2-Perfluorododecanoic acid (13C2-PFDoA) 18O2-Perfluorohexanesulfonate (18O2-PFHxS) 13C4-Perfluorooctanesulfonate (13C4-PFOS) 13C8-Perfluorooctane sulfonamide (13C8-PFOSA) Clean-up Standard 13C8-Perfluorooctanoic acid (13C8-PFOA) Recovery Standard 13C2-2H-Perfluoro-2-decenoic acid (13C2-PFOUEA) 13C4-Perfluorooctanoic acid (13C4-PFOA) Typical Retention Time (minutes) 5.0 5.8 6.2 6.6 7.0 7.4 7.9 8.5 9.0 6.3 7.2 8.2 9.9 5.0 6.2 7.0 7.4 7.9 9.0 7.2 8.2 9.9 7.0 7.3 6.9 Parent Ion Mass 213 263 313 363 413 463 513 563 613 299 399 499 498 217 315 415 468 515 615 403 503 506 421 459 417 Daughter Quantified Ion Mass Against 169 219 269 319 369 (169)1 419 469 519 569 80 (99)1 80 (99/119)1 80 (99)1 78 13c 4-p f b a 13C2-PFHxA 13C 2-PFHxA 13C2-PFHxA 13C 2-PFOA 13C 5-PFNA 13C 2-PFDA 13c 2- p f d a 13C2-PFDoA 18O2-PFHxS 18O2-PFHxS 13c 4- p f o s 13C b-PFOSA << 0IL 0IL sLL sLL CL1 C1L CL1 C1L CoO CoO CoO CoO 172 270 370 423 470 570 84 (103)1 80 (99)1 78 sLL sLL CL1 CL1 CoO CoO sLL CL1 CoO 13c 4-p f o a 13c 4-p f o a sLL C1L CoO 376 394 372 - Alternate transition within brackets, may be used if necessary to avoid interference. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 2 of 6 Page 7 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. CALIBRATION A series of at least six calibration solutions prepared in a clean reference tissue matrix similar in composition to the sample extract is used to establish initial multi-level calibration. The calibration solutions contain the analytes of interest covering the working range of the instrument together with labelled surrogate and recovery standards. A mid-level calibration solution is analyzed at least after every 20th sample to demonstrate calibration stability. All calibration solutions are processed through SPE cleanup. The concentration of the lowest level calibration standard used for quantification must exceed the method detection limit (MDL). Nominal Final Concentrations of Calibration Solutions (ng/mL) PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 13c 4- p f b a 13C2-PFHxA 13C 2-PFOA 13c 5- p f n a 13c 2- p f d a 13C 2-PFDoA 18O2-PFHxS 13C 4-PFOS 13c 8- p f o s a 13c 2- p f o u e a 13c 4- p f o a CAL A 0.125 0.125 0.125 0.125 0.125 0.125 0.125 0.125 0.125 0.25 0.25 0.25 0.125 3 3 9 3 3 3 4.5 4.5 3 2.5 3 CAL B 0.312 0.312 0.312 0.312 0.312 0.312 0.312 0.312 0.312 0.625 0.625 0.625 0.312 3 3 9 3 3 3 4.5 4.5 3 2.5 3 CAL C 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 1.25 2.5 2.5 2.5 1.25 3 3 9 3 3 3 4.5 4.5 3 2.5 3 CAL D 5 5 5 5 5 5 5 5 5 10 10 10 5 3 3 9 3 3 3 4.5 4.5 3 2.5 3 CAL E 25 25 25 25 25 25 25 25 25 50 50 50 25 3 3 9 3 3 3 4.5 4.5 3 2.5 3 CAL F 50 50 50 50 50 50 50 50 50 100 100 100 50 3 3 9 3 3 3 4.5 4.5 3 2.5 3 CAL G 125 125 125 125 125 125 125 125 125 250 250 250 125 3 3 9 3 3 3 4.5 4.5 3 2.5 3 CAL H 312.5 312.5 312.5 312.5 312.5 312.5 312.5 312.5 312.5 625 625 625 312.5 3 3 9 3 3 3 4.5 4.5 3 2.5 3 1 Concentrations shown are based on the amount added to calibration standards, with appropriate split portion (1/10, 1/2 or entire extract) cleaned up on SPE and calculated in terms of a 4 mL extract volume. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 3 of 6 Page 8 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. ANALYTE IDENTIFICATION Positive identification of target PFC, surrogate standard and recovery standards require: > 3:1 S:N for parent ion to daughter ion transition. Compound retention time must fall within 0.4 minutes of the predicted retention times from the daily Calibration Verification. Natives with labeled surrogate standards must elute within 0.1 minutes of the associated labeled surrogates. QUANTIFICATION Target compounds are quantified using the internal standard method, comparing the area of the quantification ion to that of the 13C-labelled standard and correcting for response factors. Linear quantification equations are determined from a multi-point calibration series with 1/X2 weighting fit and expressed as below: Y = slope x X + intercept Where: Y = response ratio area of Target x weight of Surr (ng) area of Surr X = weight of target (ng) , and The slope and intercept are used to convert raw peak areas in sample chromatograms to final concentrations as follows: Sample Conc. area of Target 1 ar 1 a x weight of Surr (ng) - interceptj x x area of Surr slope j Vsample size (g) j where Surr is the surrogate standard The recovery of the surrogate standard is calculated (by internal standard quantification against the recovery standard using an average RRF) and monitored as an indication of overall data quality. Final target concentrations are recovery corrected by this method of quantification. REPORTING LIMITS Concentrations and detection limits for the target analytes are reported. Typical reporting units for all data are ng/g on a wet weight basis. The following are commonly requested reporting limits: Method Detection Limit (MDL) - determined as specified by EPA Fed. Reg. 40 CFR Part 136 Appendix B (no iteration option). The 99% confidence level MDL is determined based on analysis of a minimum of 7 replicate matrix spikes fortified at 1-5 times the estimated detection limit. MDL is determined as required based on accreditation, contract and workload requirements. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 4 of 6 Page 9 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Lower Method Calibration Limit (LMCL) - determined by prorating the concentration of the lowest calibration limit for sample size and extract volume. The following equation is used. LMCL = ((lowest level cal conc.) x (extract volume))/sample size. Typical extract volume for PFCs in tissues is 4 mL. For the analysis of PFCs it is AXYS standard to report sample concentrations using the LMCL as the lower reporting limit. In cases where the SDL is higher than the LMCL, the SDL will be used as the lower reporting limit. The SDL is defined as follows: Sample Specific Detection Limit or Sample Detection Limit (SDL) - determined individually for every sample analysis run by converting the area equivalent of 3.0 times (2.5 times for EPA 1600 series methods) the estimated chromatographic noise height to a concentration in the same manner that target peak responses are converted to final concentrations. The SDL accounts for any effect of matrix on the detection system and for recovery achieved through the analytical work-up. QUALITY ASSURANCE / QUALITY CONTROL All samples are analyzed in batches. The composition of a batch is detailed on a batch sheet. Each batch has the following composition: Batch Size - Each batch consists of test samples and additional QC samples. Blanks - 5% of the samples within a batch are procedural blanks. Duplicates - 5% of the samples within a batch are analyzed in duplicate. Matrix Spike/Matrix Spike Duplicate - may be analyzed upon client request. Reference Samples - 5% of the samples within a batch are spiked reference samples. QC Specification Table: Procedural Blank Levels and OPR Recoveries Analyte Perfluorobutanoate Perfluoropentanoate Perfluorohexanoate Perfluoroheptanoate Perfluorooctanoate Perfluorononanoate Perfluorodecanoate Perfluoroundecanoate Perfluorododecanoate Perfluorobutanesulfonate Perfluorohexanesulfonate Perfluorooctanesulfonate Perfluorooctane sulfonamide (PFBA) (PFPeA) (PFHxA) (PFHpA) (PFOA) (PFNA) (PFDA) (PFUnA) (PFDoA) (PFBS) (PFHxS) (PFOS) (PFOSA) Procedural Blank Level ng/g 1 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 < 0.5 <1 <1 <1 < 0.5 Acceptable Matrix Spike (% Recovery) 2 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 70 - 130 1 Based on the default Option B extraction procedure a (2 gram sample sizesplit 2X). Procedural blank limits must be prorated accordingly for Option A or C. 2 Marginal exceedance allowance - recovery for one compound may be 60 - 140%. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 5 of 6 ^ Page 10 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. QC Specification Table: Surrogate Standard Recoveries, Calibration and Samples Surrogate Standard 13C4-Perfluorobutyric acid 13C2-Perfluorocaproic acid 13C2-Perfluorooctanoic acid 13C5-Perfluorononanoic acid 13C2-Perfluorodecanoic acid 13C2-Perfluorododecanoic acid 18O2-Perfluorohexanesulfonate 13C4-Perfluorooctanesulfonate 13C8-Perfluorooctane sulfonamide (13C4-PFBA) (13C2-PFHxA) (13C2-PFOA) (13C5-PFNA) (13C2-PFDA) (13C2-PFDoA) (18O2-PFHxS) (13C4-PFOS) (13C8-PFOSA) 1 Recovery Range 20 - 150% 40 - 150% 40 - 150% 40 - 150% 40 - 150% 40 - 150% 40 - 150% 40 - 150% 20 - 130% Clean-up Standard 13C8-Perfluorooctanoic acid (13C8-PFOA) 40 - 150% 1 Lower surrogate recoveries may be reported for individual samples where dilution analysis or spiked sample results demonstrate acceptable accuracy. QC Specification Table: Other Parameters QC Parameter Specification Instrument Sensitivity Initial Calibration (native compounds) Daily, S:N >3:1 for all analytes for lowest calibration standard. - Daily, (1/x2) weighed linear regression. - For the calculated concentrations, a maximum of 1 point per calibration solution with a maximum of 3 points for all the calibration solutions may fall within 60-140% of the actual con centration provided all remaining points, including those for PFOA, PFOS, and PFDA, fall within 70-130% of actual concent ration. - Internal guideline - correlation coefficient >0.95. Calibration curves with lower correlation coefficient values meeting all above criteria may be accepted based on batch specific QC results and professional judgement. - Surrogate recoveries must fall within the same limits as for the samples in the table above. Continuing Calibration Verification (native compounds) Every 20 samples, determined concentrations must be within 30% of actual concentrations. Surrogate recoveries must fall within the same limits as for the samples in the table above. Instrumental Carryover and Instrument Background Every Initial Calibration, Cal/Ver, or SPM: < 0.3% carryover and area response of analytes in instrument blank < 800 judged following two previous methanol blank injections. MSU-043 Rev 07, 06-Sep-2012 Summary of AXYS Method MLA-043 Rev 08 Ver 03 Page 6 of 6 Page 11 of 234 www.axysanalytical.com Weston Solutions Inc. CORRELATION TABLE PERFLUORINATED ORGANIC ANALYSIS Lab Name: AXYS Analytical Services Ltd. Project Name:Fall 2012 Tennessee River Sampling Data Package Identification: DPWG42640 Project Manager: Candice Navaroli Contract No: 4731 AXYS Method: MLA-043 Program: Tissue Samples Client Sample No. Lab Sample ID LAB BLANK OPR W G 42409-101 W G 42409-102 MSF088 MSF093 MSF008 IPF019 MSF032 IPF150 IPF154 IPF156 MSF171 MSF110 MSF126 IPF136 IPF182 MSF054 MSF064 IPF072 L19064-1 L19064-2 L19064-3 L19064-4 L19064-5 L19064-6 L19064-7 L19064-8 L19064-9 L19064-10 L19064-11 L19064-12 L19064-13 L19064-14 W G42409-103 DUPLICATE L19064-15 L19064-16 Page 12 of 234 www.axysanalytical.com Client Information (Sub Contract Lab) Client Contact: Shipping/Receiving Company: AXYS Analytical Services Ltd. Address: PO BOX 2219, 2045 Mills Road, City: Sidney State, Zip: BC, V8L 5X2 Email: Project Name: Fish Tissue Homogenization Sample Identification - Client ID (Lab ID) MSF088 (200-14181-16) MSF093 (200-14181-22) L i lc to 4 -O- Chain of Custody Record TestAmerica Sampler: _ Phone:' Due Date Requested: 12/26/2012 TAT Requested (days): WO#: Project #: 20004362 ssom . Lab PM: Young, Kirk F E-Mail: kirk.young@testamericainc.com Carrier Tracking No(s): S c 'H - H i i s - q *i0 S ~ Analysis Requested COC No: 2 0 0 -1 1 1 5 9 .1 Page; Page 1 of 1 Job#: 200-14181-1 . Preservation Codes: A-HCL B - NaOH C - Zn Acetate D - Nitric Add E - NaHS04 F - MeOH G - Amchlor H - Ascorbic Acid I - ice J - D'l Water K-EDTA L-EDA M - Hexane N - None O - AsNa02 P - Na204S Q - Na2S03 R -Na2S2S03 S-H2S04 T - TSP Dodecahydrate U - Acetone V-MCAA W - ph 4-5 Z - other (specify) Other: Sample Date 11/9/12 Sample Time Eastern Sample Matrix Type {C=Comp, (w=water, S=solid, 0=waste/il, ZKG^grab) BT=Tissue, A" Alrl Preservation ( Tissue 11/15/12 Eastern Tissue Special Instructions/Note: Shippment of samples to 3M Corporation. Shippment of samples to 3M Corporation. Possible Hazard Identification Unconfirmed Deliverable Requested: I, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished by: LA . Refiqished by: CvN'C Relinquished by: Custody Seals Intact: Custody Seal No.: A Yes A No Date: Sample Disposal ( A fee may be assessed if samples are retained longer than 1 month) Return To Client______i~ b isposal By Lab Special Instructions/QC Requirements: ^ /A1'rchive For Months Time: Company t 4 - ug.* Company Method of Shipment: Date/Time: \cu^~ Company Company Company Received by: Company Cooler Temperature(s) C and Older Remarks "Page'f3 o234 www.axysanalytical.com Client Information (Sub Contract Lab) Client Contact: Shtpping/Rceiving -C ' Company: AXYS Analytical Services Ltd. Address: PO BOX 2219, 2045 Mills Road, City: Sidney State, Zip: BC, V8L 5X2 . ' ' Phone: Email: - Project Name: Fish Tissue Homogenization S i t e : ........................... '' " Sample Identification - Client ID (Lab ID) MSF008 (200-14174-8) IPF019 (200-14174-20) MSF032 (200-14174-33) L \ D b S - 'H ,, if ~ Chain of Custody Record r m TestAmerica Sampler: Phone; . Due Date Requested: 12/26/2012 TAT Requested (days): PO #: WO #: Project#: 20004362 SSOW#: Sample Sample Date Time 11/3/12 11/3/12 11/3/12 Eastern Eastern Eastern Lab PM: Young, Kirk F E-Mail: kirk.young@iestamericainc.corn Carrier 7 racking No(s) Analysis Requested H io S ' iM i3 COC No: 200-11013.1 Page: Page 1 of 1 Job#: 200-14174-1 Preservation Codes: tH JS n 1z1 a> 1 j j V, a 05 05 Sample Matrix do 03 3f Type (C-Comp, G=grab) {w=water, S=solid, 0=waste/oil, HI - 1issue, A-Atr) iZ g || I 1 XHI CL P res erviation Code. S S Z 2s Tissue X Tissue X Tissue X SUBCONTRACT B - NaOH C - Zn Acetate D - Nitric Add E - NaHS04 F - MeOH G - Amchlor H - Ascorbic Add ! - ice W J - Dl Water o c M L-EDA oO Other: 0 d) A N - None O - AsNa02 P - Na204S Q - Na2S03 S - H2S04 U - Acetone V - MCAA W - ph 4-5 Z - other (specify) . z filf o Special Instructions/Note: V Shippment of samples to 3M Corporation. 1 1 Shippment of samples to 3M Corporation. 1 Shippment of samples to 3M Corporation. Possible Hazard Identification Unconfirmed Deliverable Requested: 1, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished b y r f (C '7 ^ Relinquished by: Gc Relinquished by: . Custody Seals Intact: Custody Seal No.' A Yes A No , Date/Time: Date/Time: Date: iH IS " Daie/Time: Sample Disposal ( A fee may be assessed if samples are retained longer than 1 month) Return To Client ^~bisposal B y Lab Special Instructions/QC Requirements: ^Archive For Months Time: Company T A ufu Company Receivedb^^^^^^ ^ Received by: IMethod of Shipment: _____ Date/Time: to g - Date/Time: Company , Company Company Received by: Date/Time: Company Cooler Temperature(s) C and Other Remarks "Page 14 df 234 www.axysanalytical.com Chain of Custody Record TestAmerica '. Client Information (Sub Contract Lab) Client Contact: Shipping/Receiving Company: AXYS Analytical Services Ltd. Address: PO BOX 2219, 2045 Mills Road, City: Sidney State, Zip: BC, V8L 5X2 Phone: t Email: ' . Project Name: Fish Tissue Homogenization Site: ' Sample Identification - Client ID (Lab ID) IPF150 (200-14182-6) IPF154 (200-14182-10) IPF156 (200-14182-12) MSF171 (200-14182-28) L H o fo M - -g - Cj Sampler: Phone: Lab PM: Young, Kirk F E-Mail: kirk.young@testamericainc.com Carrier Tracking No(s): SOZH % c\5 ............. M 4r COC No: 200-11159.1 Page: . Page 1 of 1 - Analysis Requested Job#: 200-14182-1 Due Date Requested: 12/26/2012 TAT Requested (days): , PO#: ' WO#: Project #: . 0 .z 0 tfl V V 20004362 SSOW#: Sample Date Sample Time Sample Type (C=Comp, G=grab) Matrix a> m, E V> TJ 1 (w=water, S=solid, O-waste/oil, U.. BT=Tssue, A=Alr) Preserve X ^ P e rfo rm M S/MSD (Yes or No) SUBCONTRACT Preservation Codes: A- HCL M - Hexane B - NaOH N - None C - Zn Acetate D-Nitric Acid O - AsNa02 P - Na204S FE - NaHS04 - MeOH Q - Na2S03 R - Na2S2S03 G - Amchlor S.-H2S04 H - Ascorbic Acid f - Ice T - TSP Dodecahydrate U -Acetone ip. J -D I Water K-EDTA AC'S :3Co ;v. L - EDA Other: V-MCAA W - ph 4-5 Z - other (specify) m a Z fi Special Instructions/Note: X 11/21/12 Eastern Tissue X 1 Shippment of samples to 3M Corporation. 11/21/12 Eastern Tissue X Shippment of samples to 3M Corporation, 1 11/21/12 - Eastern Tissue X Shippment of samples to 3M Corporation. 1 11/29/12 Eastern Tissue X Shippment of samples to 3M Corporation. 1 Possible Hazard Identification Unconfirmed Deliverable Requested: I, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished by: Relinquished by: . Relinquished by: Custody Seals Intact: Custody Seal No.: A Yes A No Date/Time: ti4 W , Date/Time: Date/Time: Date: __ Company Company Company Sample Disposal ( A fee may be assessed if samples are retained longer than 1 month) Return To Client_____ ^^D isposal By Lab_______ ^~krchive For Special Instructlons/QC Requirements: Months Time: R eceiv^d^^^^ Received by: Method of Shipment: Date/Time: - W Date/Time!5^ - i^ v Company Company Received by: Date/Time: Company Cooler Temperature(s) C and Other Remarks: A www.axysanalytical.com Client Information (Sub Contract Lab) Client Contact: -, Shpping/Receiving Company: AXYS Analytical Services Ltd. Address;. " PO BOX 2219, 2045 Mills Road, City: Sidney State,.Zip: BC, V8L 5X2 Project Name: Fish Tissue Homogenization Sample Identification - Client ID (Lab IP) MSF110 (200-14185-2) MSF126 (200-14185-18) IPF136 (200-14185-29) IPF182 (200-14185-41) L H C to T -tO ia Sampler: Phone: Due Date Requested: 12/26/2012 TAT Requested (days): Chain of Custody Record HT5>\ Lab PM: Young, Kirk F E-MaiS: kirk.young@tesiamericainc.com Carrier Tracking No(s): 503ei Analysis Requested Project#: 20004362 SSOWft. Sample Date "11/15/12 Sample Time Sample Type (C=Comp, G=grab) Matrix (w=water, S=solld, 0=waste/oil, BT-Tissue, AAlr Preservation Code: Eastern Tissue 11/16/12 Eastern Tissue 11/21/12 12/13/12 Eastern Eastern Tissue Tissue TestAmerica eoe No: 200-11159.1 Page: Page 1 of 1 Job #: 200-14185-1 Preservation Codes: A - HCL B - NaOH C - Zn Acetate D - Nitric Acid E - NaHS04 F - MeOH G - Amchlor H - Ascorbic Acid I - !ce J - Dl Water K-EDTA L-EDA. M - Hexane N - None O - AsNa02 P - Na204S Q - Na2S03 R -,Na2S2S03 S - H2S04 T - TSP Dodecahydrate U - Acetone V - MCAA W -.ph 4-5 Z - other (specify) Other: Shippment o f sam ples to 3M Corporation. Shippment of samples to 3M Corporation. Shippment of samples to 3M Corporation. Shippment of samples to 3M Corporation. Possible Hazard Identification Unconfirmed Deliverable Requested: l, II, III, IV, Other (specify) Empty Kit Relinquished by: Relinquished by: Relinquished by: Cric, Relinquished by: Custody Seals intact: Custody Seal No.: A Yes A No Date: Date/Time: Company Company Company Sample Disposal ( A fee may be assessed if samples are retained longer than 1 month) ' ^Return To Client______ b isposal By Lab_________* ^Archive For Special Instructions/QC Requirements: Months Time: Recived by: Method of Shipment: Date/Time: Company Received by: Date/Time: Company Received by: Date/Time: Company Cooler Temperature(s) C and Other Remarks. Page' 10 of 234 www.axysanalytical.com Client Information (Sub Contract Lab) Client Contact: Shipping/Receiving Company: AXYS Analytical Services Ltd. Address: . PO BOX 2219, - 2045 Mills Road City: Sidney State, Zip: BC, V8L 5X2 Phone: Email: Project Name: Fish Tissue Homogenization Site:. Sample Identification - Client ID (Lab ID) . " "" MSF054 (200-14179-18) L MSF064 (200-14179-29) IPF072 (200-14179-37) - \4 " . -- Chain of Custody Record \4 4 3 Sampler: ` Phone: - Due Date Requested: 12/26/2012 TAT Requested (days): Lab PM: Young, Kirk F E-Mail: kirk.young@testamericainc.com Carrier Tracking No{s): 5^ Analysis Requested SUBCONTRACT PO #: WO#: Project-#: 20004362 SSOW#: Sample Sample Date Time ^>C 11/7/12 S xC Eastern - o' Z ` Sample Type {C=comp, G=grab) ' Matrix (w=water, S-sofid, 0=waste/oii, BT=Tissue, A=Air) o mUt & a> & z Q I >p$ f(S3 Vi "m:W ,r6j Si $ li 1 1 iT 2 $. oi mXm mi Presewat on Code E X Tissue X 11/7/12 Eastern Tissue X 11/9/12 Eastern Tissue X COC No: 200-11013.1 iH o r Page: Page 1 of 1 Job#: 200-14179-1 P res erv a tio n C o d es: A-HCL B - NaOH C - Zn Acetate D - Nitric Add E -N a H S O t F- MeOH G - Amchlor H - Ascorbic Acid I - Ice j - D I Water . o: K - EDTA L - EDA 3 :C O a O ther: M - Hexane N - None O - AsNa02 P - Na204S Q -N a2S 0 3 R - Na2S2S03 S - H2S04 T - TSP Dodecahydrate U - Acetone V-MCAA W - ph 4-5 Z - other (specify) <u XJ 3 Z G Special Instructions/Note: ~ r z r = . ............ -- --------------- " Shippment of samples to 3M Corporation. 1 1 Shippmerit of samples to 3M Corporation. 1 Shippment of samples to 3M Corporation. -. Possible Hazard Identification Unconfirmed Deliverable Requested: I, II, III, IV, Other (specify) Empty Kit Relinquished'by: Relinquished by: (A Relinquished by: ..., . Relinquished by: Custody Seals Intact: Custody Seal No. A Yes A No Date/Time: <(7/ Date/Time: Date/Time: Date: Mr Sample Disposal ( A fee may be assessed if samples are retained longer than 1 month) Return To Client ^~~b/sposa/ By Lab ^~krchive For Months Special Instructions/QC Requirements: Company Company iTime: k /t Received Received by: Method of Shipment: _/ Date/Time: Date/Timesd Company Company Company Received by: Cooler Temperature(s) C and Other Remarks: Date/Time: Company ____ :....... .... \ ( ------------------------------------------------------------------------------------------------------- Paoe 17 J www.axysanalytical.com (iT l Do Not Lift Using This Tag ORIGIN ID= BTVA <802) 923-1058 ? ^ Ll ^ 0GAEgEy|lNGTON 30 COMMUNITY DRIVE InfiTH BURLINGTON, VT 05403 US S I G TM SAMPLE MANAGEMENT SHIP DATE: 07J|N4,3 ACAPDT:UG0T0081990.3964LXBCAnFAEN2e3 DIMS:U22xUxl2 IN DRY life: ,4i5 KGS BILL SENDER NO EEI 3 0 \ _______ 0CANDICE NAVAROLI n AXYS ANALYTICAL SERVICES LTD. PO BOX 2219 2045 NULLS ROAD SIDNEY BC V8L5X2 (CA) 655-5800 FedExPOtNU; d e p t ;. [)> 01 02V8L5X2 124 01 5039469544050430 FDE 297200119 007 1/1 19.9LB N PO BOX 2219 SIDNEY BC CANDICE NA Express IB the Untied SI,IB , Bcord.ne. w ill IfcJ " J1" 1" 1 mulitun. Uiviraloii bmUi w H 3 I jjj fc PiTM1'<l>1.:..--------------- 5039 4695 4405 XQ YYJA AM1 INTL PRIORITY1 ICE V8L 5X2 BC-CA YVR 1009981981191180382100503946954405 ta. !i:r Z" { m Page 18 of 234 www.axysanalytical.com AXYS Analytical Services Ltd SAMPLE RECEIVING RECORD Waybill : Date Shipped: AXYS Client & Contract # Project Number: Login Number: [Yes)/No W jAN-13 4731-Weston Solutions Waybill #: 503946954405 Date /Time Received: 08-JA N -13 11:20 Receipt No: WB14271 Received By: KSTADLER Axys Sample ID's: ] - 1- io ^ 1/5 I nn in by l/'Ac): 0 t/fS-c-- I Signature/ A th /J Condition of Shipping Container: ] Temperature upon Receipt: -30 Celcius Custody Seals: Shipping Containers n/ei! samples arrived frozen on dry ice O'fjou-l'} Intact@>/No Seal Numbers Yes/tJp> Samples Yes(/No^ Intact Yes /No Seal Numbers Yes /No Thermometer ID: 3266 Custody or Documents: Sample ID's Collection Location Date & Time Collection Collector's Name Qe)Mo (Yes)/No Yesp> (Ye^/No Yes (So) Tracking Report/Packing List: Yes (NO) Sample Tag Numbers Yes /N) Sample Type (Yes)/No Preservative Added Yes Preservation Requested Y e st Sample Tags Sample Labels Sample Labels Cross Referenced to COC Sample Tags Cross Referenced to Sample Labels Sample Tags Cross Referenced to COC Comments: A s p& / cit^ck> ^ V U - ty f'y ej) 'SaAvyW \ Yes /fid) fe|/N o (Te^l/No Yes /No Yes /No Information Agrees Information Agrees Information Agrees (Yes /No, Yes /No Yes /No A er , " i V - \T> 4 t>__I ba 1 \p f\3 s ( 9 cQ - ( as sW >*y C oci \fP\S'Ld s, 1~ tW o rv ^ (w e j) CLVe--^r \ I n e r . iS Action Taken: Page 19 of 234 www.axysanalytical.com 1Mr56 DNLL831 1V.LN3WNOHIAN3 NI H3C!31 3HX DDU9UUV4S01 te a , -r% BHniVNBIS skpU snj TeslAmerica THE LEADER IN ENVIRONMENTAL TESTING 719544 Page 20 of 234 www.axysanalytical.com $ I( AXYS AXYS Analytical Services Ltd. Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 PM Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-1 MSF088 09-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY . L19064-2 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF093 15-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv .Package ANY L19064-3 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF008 03-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-4 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT 1PF019 03-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT 09 - 42a / Page: <i of 4 Received 08-JAN-13 Due PR 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag 08-JAN-13 USD USD USD USD 1 : Plastic Bag USD USD USD USD Page 21 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. m AXYS Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 PM Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-5 MSF032 03-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-6 FC043.HALF PFCEDD , PFOS DATA PKG SAMPLE RECEIPT IPF150 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-7 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF154 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D. Package ANY L19064-8 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF156 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT Page: 2 of 4 Received 08-JAN-13 Due PR 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag USD USD USD USD Page 22 of 234 www.axysanalytical.com m AXYS AXYS Analytical Services Ltd. Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 PM Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-9 MSF171 29-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-10 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF110 15-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-11 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF126 16-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-12 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF136 21-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT Page: 3 of 4 Received 08-JAN-13 Due PR 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag USD USD USD USD Page 23 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. $)][(& AXYS , Login Chain of Custody Report (In01) Jan. 09, 2013 02:23 PM Login Number: L19064 Account: 4731 Weston Solutions Project: Axys ID versus Client Sample Identification L19064-13 IPF182 13-DEC-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-14 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF054 07-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D. Package ANY L19064-15 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT MSF064 07-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D.Package ANY L19064-16 FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT IPF072 09-NOV-12 00:00 Storage: WIF-4, Floor Tissue EDataDeliv D. Package ANY FC043.HALF PFC EDD PFOS DATA PKG SAMPLE RECEIPT Page: 4 of 4 Received 08-JAN-13 Due PR 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag 08-JAN-13 USD USD USD USD 1 Plastic Bag USD USD USD USD Page 24 of 234 www.axysanalytical.com Candice Navaroli From: Young, Charles T. [Charles.Young@WestonSolutions.com] Sent: Wednesday, January 09, 2013 5:45 AM To: Young, Kirk; Candice Navaroli Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 Just so. Thanks all. Charlie C harles T. Young Technical Director Weston Solutions, Inc. .1400 W eston Way (5-2) W est C hester, PA 19380 (610) 701-3787 cell (610) 9 52-1093 fax (610) 701-3401 charles.young@ wcstonsohiti.ons.com From: Young, Kirk [ mailto:Kirk.Young@testamericainc.com] Sent: Wednesday, January 09, 2013 8:42 AM To: Candice Navaroli Cc: Young, Charles T. Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 Im portance: High Candice, You should use the original client identifier. For sample IPF150 (200-14182-6), the original client identifier is IPF150. I hope that this is straightforward. If there are any question, please feel free to ask. Kirk KIRK YOUNG, P.E. Project Manager TestAmerica THE LEADER IN ENVIRONMENTAL TESTING 30 Community Drive, Suite 11 South Burlington, VT 05403 Tel 802,660.1990 Direct 802.923.1017 www.testamericainc.com From: Candice Navaroli rmailto:cnavaroli@axvs.com1 Sent: Tuesday, January 08, 2013 5:16 PM To: Young, Kirk Cc: Young, Charles T. Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 Hi Kirk and Charles, The samples arrived this morning safe and sound at -30 degrees C. There is a sample discrepancy for each sample that occurs and I would like to know how you wish for the samples to be logged in. The sample ID tag has: IPF150 (200-14182-6) but the COC has IPF150 (200-14182-A-6). This seems to be the same for each sample where there is an A added into each of the samples IDs. Which would you prefer we use, the sample contained ID or the client COC to log in the samples? Regards, Candice 09/01/2013 Page 25 of 234 www.axysanalytical.com Candice Navaroli (B.Sc.) Project Manager Tel.: 250-655-5800 Direct: 250-655-5839 Toil free: 1-888-373-0881 Ext.5839 Fax: 250-655-5811 Email: cnavaroli@axvs.com Axys Analytical Services Ltd. 2045 Mills Road Sidney, BC. Canada, V8L 5X2 From: Candice Navaroli Sent: Tuesday, January 08, 2013 9:27 AM To: 'Young, Kirk' Cc: Young, Charles T. Subject: RE: Homogenized Tissue - Shipment - 01/07/2013 Much appreciated, thanks Kirk. . I will update you and Charles as to the condition of the samples once they are received. Regards, Candice Candice Navaroli (B.Sc.) Project Manager Tel.: 250-655-5800 Direct: 250-655-5839 Toll free: 1-888-373-0881 Ext.5839 Fax: 250-655-5811 Email: cnavaroli@axvs.com Axys Analytical Services Ltd. 2045 Mills Road Sidney, BC. Canada, V8L 5X2 From: Young, Kirk rmailto:Kirk.YounQ(a>testamericainc.com1 Sent: Tuesday, January 08, 2013 6:37 AM To: Candice Navaroli Cc: Young, Charles T. Subject: Homogenized Tissue - Shipment - 01/07/2013 Candice, We did ship the homogenized tissue from here yesterday, January 7th, for your receipt today. The shipment was made in a single cooler under FedEx tracking number 5039-4695-4405. The attached file contains the C-O-Cs and other documentation for the shipment. Thank you for your assistance, and let me know if you should have any questions. Kirk KIRK YOUNG, P.E. Project Manager 09/01/2013 Page 26 of 234 www.axysanalytical.com TestAmerica THF. LEADER IN ENVIRONMENTAL TESTING 30 Community Drive, Suite 11 South Burlington, VT 05403 Tel 802.660.1990 Direct 802.923.1017 www.testamericainc.com CONFIDENTIALITY: This email and attachments may contain information which is confidential and proprietary. Disclosure or use of any such confidential or proprietary information without the written permission of Weston Solutions, Inc. is strictly prohibited. If you received this email in error, please notify the sender by return e-mail and delete this email from your system. Thank you. 09/01/2013 Page 27 of 234 www.axysanalytical.com www.axysanalytical.com BATCH LIST PART ONE BATCH ID: W G42409 Method ID: MLA-043 R8.03 Method ID: Method ID: Analyst: Start Date: : 10-Jan-13 Batch List Author: ISDHES! Checked By: :/F A Project Manager: Glassware Type: Glassware Treatment: Rotovap Type: 1Candice N/A !N/A N/A Matrix: Sample size; Moistures required? Lipids required? Extraction Solvent: FMS Program: Save FMS discards? Tissue 2g NO NO ;spe N/A N/A Biohazard Type: None Comments: Sample size - 2g AXYS Analytical Services Ltd Choose largest available sample for Dup Split option B (1/2 split) previous WG's associated with samples: Samples, Batch Surrogate, Recovery Spiking deiaiis Contract # Sample Wt Contract # Sample 4731 4731 4731 4731 L19064-1 L19064-2 L19064-3 L 19064-4 2.9. 2g 29 .... 2o 4731 L 19064-5 ... ? 9 <731 _19C64-6 29 4731 4731 1.19C64-7 L I9 t64-3 29 29 4731 4731 L19064-9 L19064-10 25 29 4731 L19064-11 . 2 a 4731 ; LI 9064-12 29 4731 4731 4731 L19064-13 L19064-14 L19064-15 .... 2.9.... 2.9. . ..29 . 4731 L19064-16 .....2 9 .... Wt Spiking: Product FC043.HALF FC043.HALF Before Extraction: Samples required: Surrogate_______ IL190S4-1. 2. 3, 4, 5 ,0 .7 , 8 . 9 . 1 0 . [ g ; , * J f) 11, 12, 15, T4, 15, 1 6 WG42409- | ~ T S lZ f toi,102,103____________ !40uL FC023A-SUR/LgM- !r/a^ 4 (O :WG42409-104 SAR only i20uL FC023A-SUR/ o (....' _ After Extraction: C_le_a_n__u_p_S__u_rr_o_g_a_t_e___ Recovery__________ F.V. ^ :(guy) m a w w 20uL FCD22A-SUR/2 ;50uL FC009A-REC/QI 4000 uL !20uL FC22A-SUR/ :50uL FCQ09A-REC/ 4000 uL U-vial Instructions and Comments: pPR O P SNAP W i SAR is spiked w ith 1i2 o f th e SUR used in the samples. CHECK THAT UN PVCD SAIL ALSO USES M2 SUFt :pvcD:pPROP SNAPW / FSA-004 Rev 16, 16-Feb-2010 i m Page 1 Page 29 of 234 www.axysanalytical.com BATCH LIST PART TWO BA TC H ID: W G424D9 Type and Analysis QC QC Samples Weight______ required for: FCD43 HAL? Authentic spiking: WG42409-101 BLANK 1G CANADIAN SPRINGS WATER AXYS Analytical Services Ltd QC samples, Authentic Spiking arsa Comments Comments: Type and Analysis QC QC Samples Weight required for: Authentic spiking: Comments: WG424Q9-102 SPM OPT. A: 1G SC52G5 r~ L500-30 (15T-REF). ! OPT, B&C: 16 ; SC5211 (12T-RF) 1320uL FC03SB-AUT/ WG42409-103 DUP CHOOSE LARGEST SAMPLE AND ! UPDATE LiMS WG42409-104 SAR SAR jFC043 HALF : . ' W.20uL f C023A-SU ........... jl60uLFC039B-AUT7 . =20-uL FC022A-SUR/ 50uL FCG09A-REC/ _____ FV:GOG uL FSA-04 Rev 16, 16-Feb-2010 Page 2 Pagfc30 of 234 www.axysanalytical.com Sample Labeling Information: Batch ID: WG42409 Method: MIA-043 R8.03 AXYS Analytical Services Ltd Date: 10 _____ Analyst: ft* Contract #:_____ 473t Sample ID L19064-1 L19064-2 L19064-3 Sample Type and Description ftAft M irrt a('Vi' Tf _ Q/YQM fYOt^k <2^ TO ..... UQ Original Labelling hs^ogi 'jtx toXoftAiZ, L-Jj HS 0/rfrA< ' 2-0 ~T-/W j /- fl -t& tfSp& Mip o t f r JLIM * ^4 * ieZfoMiZ nrk^-Joo-mS'fi^i M f en& 4 k in s LIMS label correct as per client T a 0 L19064-4 L 19064-5 L19064-6 L 19064-7 L19064-8 L19064-9 L19064-10 L19064-11 3T-&U m o n -t A /? f r rpu m P it Pp-P--/rfr" --- ../.n..o..l.'Sf...... .. ... --""----------- Pirtk (noni; 1 Of&U m o t f t 77-- V---------- r^ex'sf- ^ frfrfs-e-ce_ Ts arw m >rsf foiMSL. /w t% v f M!?# ~-A -2 0 f f o i j n f f 01% Z a o -lW W '-A -lS S fr o 3 2 X p ifrS ^ 2{?0a 4-|^2" f r - k %Pf-iSo f r P i f Hfr'flet J2 o -i4 '[(f2 .-A -/ > i p f /Sfr Iff >n^f'AUr 200 < fa - /v - ,- 2, r p p i fr fH O n^'A ^t- (V \< fr|! 20 0 -1 *** rt^ fr/f /^^'aU - 2 A~'2- HS f i a f y OrfrCAifr-. z o o - t m S b - A 'i ^ H iP i/o ' M s fr LlKS LlHS I/HS IZ X S TrMi U ms HKS B 0 0 Ei fef & ieT sd L19064-12 L19064-13 j V&JM.W .w&<t4-- ptaIa .f tnotsir /T rtio tM/OtX-f jf 4'f`ssc<. L19064-14 L19064-15 L19064-16 In ? fr i `P cA fr 'A o tsV I f f life_________________________________________ O rfrC A ^ : 2 o o - l i f | g 5 " A ~ 2 - i [T tffffti O v fr fr a f-. l o o - ^ k i S i5 " ' A ~ m V l&Z. LM a |\A<,f 0<frV M-frt, to T fiW ytl IH5 C fr^ A .4 ; x O -\H \A A --A"48 HSfroH- n s v o h iL On-WAcJit 7.00 - - A -x R \V V S V oGH- i s f o ifr O r^frft '- 200 A " V A Xfr- o fr-fr tin s 4^ Ef ^ / FW O-305 Rev 07, 1S-Dec-2012 4 frReviewed by date \n 'X Pace I r t of Pag"3T of 234 www.axysanalytical.com AXYS A nalytical S ervices Ltd Sample Labeling Inform ation: Batch ID: W G 4 2 4 0 9 Method: MLA-043 R8.03 Date: _______ Analyst: Sample ID Sample Type and Description Original Labelling WG42409-101 (BLANK) WG42409-102 (SPM) WG42409-103 (DUP) Uo (U U r ) WG42409-104 (SAR) c ( cJ cw U Veiling rvt.PiW Kuu-k j frficedunl U a4<. r uwst/%>v\ V ]fMitA ^ M v i'V C ( 4 z r - f c e p IWotoVr-x Hr; (hron. *>an t -------1-----------------V -- f-- -jf-----M A SS *>* 4 - i-\ -- ---- -------- /IM S : A fS f /) n W .' -V -- ............ _ .......... FWO-305 Rev 07, 18-Dec-2012 A 1\lOovfAReviewed by_ date Contract #: NONE iO~ecfai/Y> 4 ^ ? 6 A UMS label correct as per client p-ipeZy EK .. ......... -- ----- ------- *-------- ---------- - C L - ................................ n \ V> Page Y u o f_j _ Page 32 of 234 www.axysanalytical.com AXYS Analytical Services Ltd S a m p le W t & M o is tu re s : Batch ID: WG42409 Method: m la-043 R8.03 Date: S am ple ID L19064-1 Tare (a) Tare + W et j Analyst: { & Balance or Syringe # M oisture (SLA-015) (all w eights in gram s) Tare + dry Tare (b) Tare + wet Tare + dry Sample Wt or Vot (SLA-0B5) (g or mL) Backup (g) L19064-2 2,-1 f L19064-3 L19064-4 2 - 0 2- 'T-'/aO L19064-5 L19064-6 -2 - 1 O ? L19064-7 L19064-8 L19064-9 -v/sc? L19064-10 2.. c l . ^tw L19064-11 L19064-12 'Ts- L19064-13 L19064-14 7,77 7 -Ob ^/U O ."vj eO& L19064-15 L19064-16 2 ' a >{$9 WG42409-101 (BLANK) ( iO j -- W G42409-102 (SPM) M oistures Into oven; Date: E xtra ctio n Info rm ation: Drvinq; Dryinq Time: Extraction Solvent used: Begin: Date: FW O-306 Rev 08, 02-Jan-2011 Time: Soxhlet: mins T im e : Analyst: Dean Stark: Analyst: Analyst: Out of Oven: Date: Steam Distillation: Volume; End: Date: Reviewed by _ _ date ............. ______Tim e:____ Base Digest: R e a g e n t:__ mL T lm e : 'l.fo Analyst;______ _ Voi: m l Analyst: 1C Paqe 'M K j of ' ( _ -------PagT~3Tof 234 www.axysanalytical.com AXYS Analytical Services Ltd S am ple W t & M o is tu re s : Batch ID: WG42409 Method: m la-043 R8.03 Date: j Sam ple ID W G42409-103 (DUP) W.G2408-43-(BP)-" '' W G42409-104 (SAR) Tare (a) Tare + W et \ Analyst: j - f ^ Balance or Syringe # % M oisture (SLA-015) (all w eights in gram s) Tare + dry Tare (b) T are + wet Tare + dry Sample Wt or Vol (SLA-085) (g or mL) Backup (g) M oistures into oven: Date: _ E xtraction Info rm a tio n : Drying: Drying Time: _ Extraction Solvent used Begin: D ate:_________ FWO-306 Rev 08, 02-Jan-2011 __Tim e: _ Analyst: Soxhlet: _ __ Dean Stark: mins A n a ly s t:______ T im e : Analyst: Out of Oven: Date: Steam Distillation: V o lu m e :_____ End: Date: Reviewed by date \ l \A T im e: Base Digest: R e a g e n t:___ mL Time: Analyst:^ Vol; mL Analyst: Page m -t Page 34 of 234 www.axysanalytical.com S piking Sheet: Batch ID: WG42409 AXYS Analytical Services Ltd Method: MLA-043 R8.03 Sam ple ID L19064-1 L19064-2 L19064-3 L19064-4 L19064-5 L19064-6 119064-7 L19064-8 L19064-9 L19064-10 LI 9064-11 L19064-12 L19064-13 L19064-14 L19064-15 L19064-16 WG42409-101 (BLANK) WG42409-102 (SPM) Sample ID 1i \ t i i f 7 . ' { o q - 1 Z 5Pnn Before extraction Suit. t>\ Date: le>~JA-uA Voi.: y-o yL Svrinae#: Ampoule: %r- Analyst: Time I1U I1 2 > \\Z~b a z 'h 117.1lJb )17P> i:? i/7 ,8 \iUP> II? 1 IM WzA i!?A (hA [M ll3o Witness ~DL UO U UQU &t>U hL lULs ^)C' & $ )u mi evts Before extraction Sum. ID: TM Syringe#: Ampoule: Analyst: uL Cleanup 3rd. Cleanup Std. Sum.: f m i A S m ZSurr.: Date: n'R3M.l5 Date: 4 voi.: 20 uL Voi.: uL S vrinae#: <$Ui> Svrinae#: Ampoule: 4 . Ampoule: Start tim e>^:5"2' Start time: Fin. time: >,$:< V Fin. tim e:------- ^ Time Witness Analyst: \f j V Analyst: Recovery Recovery Sum.: pCa&fA'fZd^oj Sum.: Date: U Date: Vol.: Co uL F.V.: Luyoo uL Voi.: F.V.: Svrinqe#: SAi'-F Svrinqe#: Ampoule: t'f' Ampoule: r^ ta rt time: at.-?*5' Start time: fh. time: i 7c !>5 Fin. time: Analyst: V V V Analyst: uL uL . yf V V \/ VV V ________ v;______ V \/ \/ 1/ V V \i V \f VV VV V _______ v _______ VV VV % Authentic Std: ptoZcl R -A D T /0 7 Date: / cX7Aa j i 3 Voi.: UL Svrinae#: Ampoule#: R Analyst: H-jn Time Witness Authentic Std: R o 3 4 B ~ A i/C > t*2 - Date: Vol.: U c t pL Svrinqe#: " u T Ampoule#: O Analyst: VhfiJi. Time Witness Authentic Std: Date Voi. Syringe# Ampoule# Analyst: Time PL Witness e o ts -- -- ____________ 1 2 - C _______________ __________ FWO-310 Rev 08, 16-Feb-2010 Reviewed bv Vi4 ^ - date U jS U ^ Pane D^<=,AAqC)A][>f(<O" www.axysanalytical.com AXYS Analytical Services Ltd S p ikin g Sheet: Batch D: WG42409_____________ M ethod: MLA-Q43 R8.03 SLA-017 W itness nam e/initials: --Lo A ^ x ^ t^ /^ *pL-- S a m p le ID Before extraction Surr. 1 D : C & 2 ? > A -J u iz b [ Date: to;TA/di3> Voi.: t / b pL Svrinqe#: |4 Ampoule: ' f ' Analyst: Before extraction Surr. ID: f C -c fZ z A -S tfi/o i Date: loTT/tAJ3 Vol.: > 0 pL Svrinae#: 4Vfc>'l-- Ampoule: Analyst: Cleanup Std. S u rr.iftQ ^ It# ^ Date: n Gm U Voi.: i- o pL Svrinqe#: * V * Am poule: 4 S tart tim e ^ vSx Fin. time: oo'-ttfp Cleanup Std. Surr.: Date: Voi.: uL Svrinqe#: A m p o u le : Start tim e :_____ Fin. time: Recovery Surr.: Date: 11 2 14 ^3 Vol.: o pL F.V.: 4^ pL Svrinae#: {?i Ampoule: 1S' Start time: H ' o Fin. tim e: f2;5S Recovery Surr.: Date: Voi.: F.V.: Svrinqe#: Ampoule: Start time: Fin. time: uL pL f i W G 42409-103 (DUP) W G 42469=f63~(PtJP)-------- Time H 5o Witness i t> L -- -------- ------- Time ---- Witness -- -- Analyst: V ---- * Analyst: Analyst: V Analyst: W G 42409-104 (SAR) ---- I O sJ _________V________ Sample ID Authentic Std: Date: Voi.: Svrinqe#: Ampoule#: Analvst: Time uL Witness Authentic Std: Date: Voi.: Svrinqe#: Ampoule#: Analvst: Time uL Witness Authentic Std Date ` Voi. : Syringe# Ampoule# Analyst Time PL Witness FWO-310 Rev 08, 16-Feb-2010 Reviewed date_ h 1C Page . Oi Page 36 of 234 www.axysanalytical.com AXYS Analytical Services Ltd Extract Splitting Sheet: Batch ID: W G 4 2 4 0 9 _______ Balance #: Method: m l a -043 R8.03 Sample ID L19064-1 119064-2 L19064-3 L19064-4 L19064-5 L19064-6 L19064-7 L19064-8 L19064-9 L19064-10 L19064-11 L19064-12 L19064-13 L19064-14 L19064-15 119064-16 W G 42409-101 (BLANK) W G42409-102 (SPM) W G 42409-103 (DUR) W G 42409-103 (DUP) C-tube (column a) C-tube * total extract (column b) Total Extract (b-a) Portion + c-tube (column c) 4 -' h f 94____ 6a \ "4 ' V f 2' Analyst: Date: 1[ 3 ^ 1 ^ .T im e: c?^~-So Weight (g) Portion: (c-a) Portion: f/'T- (direct wt) 3-a * 3-<$> Portion: (direct wt) Portion: (direct wt) 1>'0 i.Ro 3-b o f 6Mr -- -- .-- -- -- Portion: (direct wt) FWO-311 Rev 06. 1-O ct-2010 Reviewed by _ Date 1C Paqe M ' of . ~ ' --Page_3 7 of 234 www.axysanalytical.com AXYS Analytical Services Ltd LC M ethods W o rksh e e t Workgroup: WG42409______ Method: MLA-043 R8.Q3 Sample Preparation and Extraction: Analyst: ______ Date: H Jau!3 Fraction: Reagent Lot# Voi. Time mL Vol. Tim e mL 50% formic acid 0.05 M KOH in MeOH 3% acetic acid 0.3% (NHaOH)/ 99% MeOH Reagent water ty p e rX 'C Carbon (50 mg ) 32 lo (OO o 8 : t acetonitrile (CHaCN) Formic add cone, NH^OH cone. EDTA 0.14 M Na2HPOi buffer pH 2 HCI cone. ammonium acetate buffer 1 M, pH 5.0 ammonium acetate buffer 1 M, pH 6.5 ammonium acetate buffer 1 M, pH 4.0 Phosphate buffer 1 M, pH 6.0 enzyme ft- glucoronidase/suifatase enzyme ft- giucoronidase 5% NH4 OH in water H2 0 type 5% borate buffer pH 9 0,012 g/mL FMOC-CI Acidified water with HCI (pH ) 3:1 MlBE:MeOH Sodium acetate buffer 0.5M, pH 4 10% acetate buffer pH 4 EDC.HCI 140mM KOH in 80:20 MeOH: Water MeOH Vol. Time mL Shake/Tippe, Analyst: Date: / o J n t/t1 T, Time: / Z- : #times: Q [ Derlvatization Analyst: Date: Start time: End time: M illipo re : Analyst: Date: Time: Filtration: Analyst: Date: Time: Extract pH check/adjust 1- pH Date: ^ Time: f t ^ Analyst: 2. pH Date: Time: Analyst: 3. pH Date: Time: Analyst: Incubation: Analyst: Date: Start time: End time: Manifold # f 1f P 2 Analyst Date: ff&&***> Sample ID Position L19064-1 L19064-2 L19064-3 L19064-4 f/ z 2 L19064-5 L19064-6 L19064-7 L19064-8 L19064-9 j pI ^ 1 r fo If lX L19064-10 L19064-11 L19064-12 L19064-13 L19064-14 L19064-15 L19064-16 ^ WG42409-101 (BLANK) WG42409-102 (SPM) WG42409-103 (DUP) WG42409-1G3 (DUP) WG42409-14 (SAR) ! Vi- iL --X y fo \ il fX l if I I #filter units FWO-333 Rev 04, 26-Oct-2011 Reviewed by: 5Page of ^ ^ ][i^ Page 38 of 234 www.axysanalytical.com AXYS Analytical Services Ltd LC M e th o d s C le a n u p W o r k s h e e t W orkgroup: W G 42409______ Method: m la -043 R8.03 Fraction Analyst: SPE Pre-Elution/ Conditionirig : Date: / A nalvst: y-H l SPE type: t^ A )c Lot#: Reagent Lot V o i. Time # mL 0.1 M Formic acid 0.3% NH4OH/ 99% MeOH 1: MeOH I JxM 5 " 0% T'b 0 C1 & 2: MeOH Reagent water type: ........ Acidified water HjO Type (with HCI) pH DCM 2:30:68 Formic acid: Acetonitriie:MtBE 2% NH4 OH in water H?0 Type Wash: Load Sample: ^ Reagent Reagent water type: 50% 0.1 M Formic acid/ 50% MeOH 0.1 M Formic acid 0.3% basic (NhLOH)/ 99% CS water Lot # /Sty 55" 20% MeOH/ 80% 0.1 M Formic acid Voi. Time mL 5 X 1Z z lS I2 - ^ 7^ 31* " ( J u Acidified water HjOTvpe (with HCi) pH 0.1 M HCi/ 5% MeOH Hexane 1: Acetonitrife (CH3 CN) 2: Acetonitrile (CH3 CN) 0.1 M HCI/ 5% MeOH MtBE 10:20:70 NH4OH: MeOH: MtBE Acetonitrile (CH3 CN) 5%MeOH/95% water H20 Type 2% NH,,OH in water. H20 Type Hexane MtBE DCM 0.5M Sodium Acetate Buffer pH 4 1:1 Acetonitrile: Acetone 5% Acetonitrile in water H20 Type MeOH Elution: Reagent MeOH 0.3% NH4OH/ 99% MeOH Carbon (optional) 1:1 Acetone:MeOH 2 % Formic acid in MeOH Lot Voi. Time # mL 4 l^vOO Acetonitrile (CH3 CN) Hexane 2:30:68 Formic acid: Acetonitrile: MtBE DCM 10:20:70 NH4OH: MeOHtMtBE 1:1 DCM:MeOH 3:1 MtBE: MeOH Ethyl acetate 9:1 MtBE: MeOH 1:1 Acetonitrile: Acetone 2 % Formic acid in MtBE Reconstitute for jjvial: Date: Analvst: Reagent 0.1% Formic acid buffer 1: MeOH 2: MeOH 3: MeOH 0.3% NH4OH/ 99% MeOH 70:30 MeOH: water HjO Type Lot Voi. Time # mL / / / / / / / 1:1 MeOH: 0.1% / acetate buffer / 1:1 CH3 CN: / Ammonium acetate, Acetic acid buffer 1:1 0.1% Acetic/acid in CHaCN:Water HsO Type / 1:1 0.2% Acetic acid in CH3 CN:Water H20 Type / Reagent wter type:___ I / j / / ! / _________ / MeOH= Methanol; MtBE= Methyl-l-butyi ether; DCM= Dichloromethane; MeCN= CH3CN= Acetonitrile; NH4OH= Ammonium hydroxide (30% aqueous unless otherwise spec.); CS= Canadian Springs FWO-334 Rev 03, 18-Sep-2012 Reviewed by: 0 3 ^Date: Page ^ of ^ * ) ] Page 39 of 234 www.axysanalytical.com Notes: Batch ID; W G 4 2 4 0 9 Method: MLA-Q43 R8.03 AXYS Analytical S ervices Ltd Date: Analyst Fraction Notes: Reviewed by _____ _ date FWO-314 Rev 04. 13-May-2008 Page PagF40 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF088 Sam ple C ollection: 0 9 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-1 M atrix : TISSUE Sam ple Size: 2.05 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 18:09:13 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: F C 3 G _ 0 1 3 S: 21 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.488 (L) U 0.488 (L) U 0.488 (L) U 0.488 (L) U 0.488 (L) U 0.488 (L) 0.540 0.488 (L) 0.721 0.488 (L) U 0.488 (L) U 0.976 (L) U 0.976 (L) 56.6 0.976 (L) U 0.585 (L) 7:59 8:35 8:17 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L 19064- 1_Form1 A_FC3G_013S21_SJ1554629.html; Workgroup: W G42409;DesignID: 1980 ] Page 41 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-1_Form1A_FC3G_013S21_SJ1554629.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF088 Sam ple C ollection: 0 9 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-1 M atrix : TISSUE Sam ple Size: 2.05 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 18:09:13 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: F C 3 G _ 0 1 3 S: 21 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 24.0 99.9 24.0 27.5 114 72.0 80.0 111 24.0 28.5 119 24.0 23.2 96.6 24.0 19.9 83.0 24.0 15.4 64.3 36.0 31.0 86.2 36.0 35.8 99.3 5:14 6:21 7:06 7:29 7:59 9:11 9:58 8:21 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 15.0 125 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-1_Form2_FC3G_013S21_SJ1554629.html; Workgroup: WG42409; Design ID: 1980 ] Page 42 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-1_Form2_FC3G_013S21_SJ1554629.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF093 Sam ple C ollection: 1 5 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-2 M atrix : TISSUE Sam ple Size: 2.11 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 18:26:14 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 22 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.474 (L) U 0.474 (L) U 0.474 (L) U 0.474 (L) U 0.474 (L) U 0.474 (L) 1.67 0.474 (L) 1.26 0.474 (L) 2.72 0.474 (L) U 0.948 (L) U 0.948 (L) 432 0.948 (L) 4.07 0.569 (L) 7:59 8:35 9:11 8:21 9:59 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-2_Form1A_FC3G_013S22_SJ1554630.html; Workgroup: W G42409;DesignID: 1980 ] Page 43 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-2_Form1A_FC3G_013S22_SJ1554630.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF093 Sam ple C ollection: 1 5 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-2 M atrix : TISSUE Sam ple Size: 2.11 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 18:26:14 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC 3G _013 S: 22 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 23.9 99.4 24.0 28.2 118 72.0 76.5 106 24.0 27.4 114 24.0 21.5 89.8 24.0 21.5 89.5 24.0 16.8 70.2 36.0 27.0 75.0 36.0 33.5 93.1 5:14 6:22 7:06 7:29 7:59 9:11 9:58 8:21 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 15.6 130 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-2_Form2_FC3G_013S22_SJ1554630.html; Workgroup: WG42409; Design ID: 1980 ] Page 44 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-2_Form2_FC3G_013S22_SJ1554630.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF008 Sam ple C ollection: 0 3 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-3 M atrix : TISSUE Sam ple Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 18:43:07 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 23 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) 0.577 0.495 (L) 0.581 0.495 (L) U 0.495 (L) U 0.990 (L) U 0.990 (L) 186 0.990 (L) 2.25 0.594 (L) 7:59 8:33 8:17 9:59 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-3_Form1A_FC3G_013S23_SJ1554631.html; Workgroup: W G42409;DesignID: 1980 ] Page 45 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-3_Form1A_FC3G_013S23_SJ1554631.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF008 Sam ple C ollection: 0 3 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-3 M atrix : TISSUE Sam ple Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 18:43:07 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 23 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 26.5 110 24.0 28.9 120 72.0 73.8 102 24.0 27.8 116 24.0 23.2 96.8 24.0 23.3 97.0 24.0 15.6 64.8 36.0 29.0 80.7 36.0 36.9 103 5:11 6:20 7:06 7:29 7:59 9:09 9:58 8:17 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 14.9 124 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-3_Form2_FC3G_013S23_SJ1554631.html; Workgroup: WG42409; Design ID: 1980 ] Page 46 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-3_Form2_FC3G_013S23_SJ1554631.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF019 Sam ple C ollection: 0 3 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-4 M atrix : TISSUE Sam ple Size: 2.07 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 19:00:08 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G_013 S: 24 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.483 (L) U 0.483 (L) U 0.483 (L) U 0.483 (L) U 0.483 (L) U 0.483 (L) 0.590 0.483 (L) 0.648 0.483 (L) U 0.483 (L) U 0.966 (L) U 0.966 (L) 10.2 0.966 (L) 0.846 0.580 (L) 7:59 8:33 8:17 9:58 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-4_Form1A_FC3G_013S24_SJ1554632.html; Workgroup: W G42409;DesignID: 1980 ] Page 47 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-4_Form1A_FC3G_013S24_SJ1554632.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF019 Sam ple C ollection: 0 3 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-4 M atrix : TISSUE Sam ple Size: 2.07 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 19:00:08 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G_013 S: 24 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 13.7 57.3 24.0 28.3 118 72.0 73.6 102 24.0 26.5 111 24.0 23.2 96.6 24.0 19.3 80.3 24.0 10.4 43.5 36.0 34.4 95.5 36.0 35.3 98.2 5:15 6:21 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFO A 12.0 14.2 118 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-4_Form2_FC3G_013S24_SJ1554632.html; Workgroup: WG42409; Design ID: 1980 ] Page 48 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-4_Form2_FC3G_013S24_SJ1554632.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF032 Sam ple C ollection: 0 3 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-5 M atrix : TISSUE Sam ple Size: 2.13 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 19:17:09 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 25 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.469 (L) U 0.469 (L) U 0.469 (L) U 0.469 (L) U 0.469 (L) U 0.469 (L) 1.28 0.469 (L) 1.23 0.469 (L) 0.649 0.469 (L) U 0.939 (L) U 0.939 (L) 56.1 0.939 (L) U 0.563 (L) 8:03 8:35 9:11 8:21 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-5_Form1A_FC3G_013S25_SJ1554633.html; Workgroup: W G42409;DesignID: 1980 ] Page 49 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-5_Form1A_FC3G_013S25_SJ1554633.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF032 Sam ple C ollection: 0 3 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-5 M atrix : TISSUE Sam ple Size: 2.13 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 19:17:09 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 25 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 29.5 123 24.0 32.3 135 72.0 82.0 114 24.0 28.0 117 24.0 22.4 93.4 24.0 24.2 101 24.0 17.6 73.2 36.0 35.5 98.5 36.0 35.0 97.2 5:12 6:22 7:06 7:29 7:59 9:11 9:58 8:21 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 15.2 127 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-5_Form2_FC3G_013S25_SJ1554633.html; Workgroup: WG42409; Design ID: 1980 ] Page 50 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-5_Form2_FC3G_013S25_SJ1554633.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF150 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-6 M atrix : TISSUE Sam ple Size: 2.21 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 19:34:02 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 26 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.452 (L) U 0.452 (L) U 0.452 (L) U 0.452 (L) U 0.452 (L) U 0.452 (L) 0.984 0.452 (L) 0.955 0.452 (L) 0.559 0.452 (L) U 0.905 (L) U 0.905 (L) 57.8 0.905 (L) 3.87 0.543 (L) 7:59 8:35 9:11 8:17 9:59 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-6_Form1A_FC3G_013S26_SJ1554634.html; Workgroup: W G42409;DesignID: 1980 ] Page 51 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-6_Form1A_FC3G_013S26_SJ1554634.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF150 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-6 M atrix : TISSUE Sam ple Size: 2.21 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 19:34:02 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 26 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 22.2 92.3 24.0 26.7 111 72.0 73.6 102 24.0 24.8 103 24.0 22.2 92.4 24.0 20.4 85.1 24.0 11.9 49.7 36.0 35.8 99.5 36.0 34.9 96.9 5:16 6:20 7:06 7:29 7:59 9:11 9:59 8:17 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 15.1 126 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-6_Form2_FC3G_013S26_SJ1554634.html; Workgroup: WG42409; Design ID: 1980 ] Page 52 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-6_Form2_FC3G_013S26_SJ1554634.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IP F 1 5 4 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-7 M atrix : TISSUE Sam ple Size: 1.99 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 19:51:03 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 27 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.503 (L) U 0.503 (L) U 0.503 (L) U 0.503 (L) U 0.503 (L) U 0.503 (L) 0.701 0.503 (L) 0.609 0.503 (L) U 0.503 (L) U 1.01 (L) U 1.01 (L) 14.5 1.01 (L) U 0.603 (L) 7:59 8:33 8:17 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-7_Form1A_FC3G_013S27_SJ1554635.html; Workgroup: W G42409;DesignID: 1980 ] Page 53 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-7_Form1A_FC3G_013S27_SJ1554635.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IP F 1 5 4 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-7 M atrix : TISSUE Sam ple Size: 1.99 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 19:51:03 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 27 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 26.8 112 24.0 27.5 115 72.0 73.0 101 24.0 25.8 107 24.0 22.0 91.7 24.0 13.8 57.3 24.0 10.4 43.5 36.0 33.8 93.9 36.0 34.3 95.2 5:12 6:21 7:06 7:29 7:59 9:09 9:58 8:17 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 15.0 125 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-7_Form2_FC3G_013S27_SJ1554635.html; Workgroup: WG42409; Design ID: 1980 ] Page 54 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-7_Form2_FC3G_013S27_SJ1554635.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF156 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-8 M atrix : TISSUE Sam ple Size: 2.12 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 20:07:56 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 28 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.472 (L) U 0.943 (L) U 0.943 (L) 12.3 0.943 (L) 1.74 0.566 (L) 8:17 9:56 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-8_Form1A_FC3G_013S28_SJ1554636.html; Workgroup: W G42409;DesignID: 1980 ] Page 55 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-8_Form1A_FC3G_013S28_SJ1554636.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF156 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-8 M atrix : TISSUE Sam ple Size: 2.12 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 20:07:56 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC 3G _013 S: 28 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 19.4 80.9 24.0 26.8 112 72.0 75.4 105 24.0 26.6 111 24.0 24.5 102 24.0 17.6 73.3 24.0 11.1 46.1 36.0 34.3 95.2 36.0 36.5 102 5:12 6:21 7:06 7:29 7:59 9:09 9:56 8:17 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 14.6 122 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-8_Form2_FC3G_013S28_SJ1554636.html; Workgroup: WG42409; Design ID: 1980 ] Page 56 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-8_Form2_FC3G_013S28_SJ1554636.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF171 Sam ple C ollection: 2 9 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-9 M atrix : TISSUE Sam ple Size: 2.24 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 20:24:57 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 29 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.446 (L) U 0.446 (L) U 0.446 (L) U 0.446 (L) U 0.446 (L) U 0.446 (L) 0.905 0.446 (L) 0.942 0.446 (L) 0.600 0.446 (L) 2.04 0.893 (L) U 0.893 (L) 345 0.893 (L) 55.4 0.536 (L) 8:03 8:35 9:11 6:27 8:21 9:59 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-9_Form1A_FC3G_013S29_SJ1554637.html; Workgroup: W G42409;DesignID: 1980 ] Page 57 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-9_Form1A_FC3G_013S29_SJ1554637.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF171 Sam ple C ollection: 2 9 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-9 M atrix : TISSUE Sam ple Size: 2.24 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 20:24:57 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC 3G _013 S: 29 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 30.4 127 24.0 31.9 133 72.0 73.5 102 24.0 22.4 93.2 24.0 22.2 92.6 24.0 20.7 86.4 24.0 15.2 63.2 36.0 28.5 79.1 36.0 35.8 99.5 5:12 6:21 7:06 7:29 7:59 9:11 9:59 8:21 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 14.7 123 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-9_Form2_FC3G_013S29_SJ1554637.html; Workgroup: WG42409; Design ID: 1980 ] Page 58 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-9_Form2_FC3G_013S29_SJ1554637.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF110 Sam ple C ollection: 1 5 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-10 M atrix : TISSUE Sam ple Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 20:41:50 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 30 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) 0.839 0.495 (L) 0.820 0.495 (L) U 0.495 (L) U 0.990 (L) U 0.990 (L) 22.0 0.990 (L) U 0.594 (L) 7:59 8:33 8:17 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-10_Form1A_FC3G_013S30_SJ1554638.html; Workgroup: WG42409; Design ID: 1980 ] Page 59 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-10_Form1A_FC3G_013S30_SJ1554638.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF110 Sam ple C ollection: 1 5 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-10 M atrix : TISSUE Sam ple Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 20:41:50 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 30 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 31.1 129 24.0 34.3 143 72.0 78.7 109 24.0 28.7 119 24.0 25.1 104 24.0 25.9 108 24.0 18.7 78.0 36.0 37.1 103 36.0 36.0 100 5:10 6:21 7:06 7:29 7:59 9:09 9:58 8:17 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 14.2 119 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-10_Form2_FC3G_013S30_SJ1554638.html; Workgroup: WG42409; Design ID: 1980 ] Page 60 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-10_Form2_FC3G_013S30_SJ1554638.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF126 Sam ple C ollection: 1 6 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-11 M atrix : TISSUE Sam ple Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 20:58:51 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: F C 3 G _ 0 1 3 S: 31 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) 0.895 0.481 (L) 0.710 0.481 (L) U 0.481 (L) U 0.962 (L) U 0.962 (L) 43.9 0.962 (L) U 0.577 (L) 7:59 8:33 8:17 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-11_Form1A_FC3G_013S31_SJ1554639.html; Workgroup: WG42409; Design ID: 1980 ] Page 61 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-11_Form1A_FC3G_013S31_SJ1554639.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: CLIENT SAM PLE NO. MSF126 Sam ple C ollection: 1 6 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-11 M atrix : TISSUE Sam ple Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 20:58:51 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: F C 3 G _ 0 1 3 S: 31 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S V 24.0 43.7 182 V 24.0 37.8 157 72.0 81.0 112 24.0 31.0 129 24.0 27.6 115 24.0 24.4 102 24.0 19.6 81.8 36.0 41.3 115 36.0 41.5 115 5:10 6:20 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFO A 12.0 15.2 127 7:06 (1) Where applicable, custom lab flags have been used on this report; V = surrogate recovery is not within method/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-11_Form2_FC3G_013S31_SJ1554639.html; Workgroup: WG42409; Design ID: 1980 ] Page 62 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-11_Form2_FC3G_013S31_SJ1554639.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF136 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-12 M atrix : TISSUE Sam ple Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 21:15:52 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 32 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.481 (L) U 0.962 (L) U 0.962 (L) 4.12 0.962 (L) U 0.577 (L) 8:17 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-12_Form1A_FC3G_013S32_SJ1554640.html; Workgroup: WG42409; Design ID: 1980 ] Page 63 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-12_Form1A_FC3G_013S32_SJ1554640.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF136 Sam ple C ollection: 2 1 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-12 M atrix : TISSUE Sam ple Size: 2.08 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 21:15:52 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC 3G _013 S: 32 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 31.0 129 24.0 32.0 133 72.0 79.9 111 24.0 26.9 112 24.0 25.9 108 24.0 20.1 83.7 24.0 11.8 49.2 36.0 41.3 115 36.0 36.2 101 5:12 6:20 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFO A 12.0 15.6 130 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-12_Form2_FC3G_013S32_SJ1554640.html; Workgroup: WG42409; Design ID: 1980 ] Page 64 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-12_Form2_FC3G_013S32_SJ1554640.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IP F 1 8 2 Sam ple C ollection: 1 3 -D e c -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-13 M atrix : TISSUE Sam ple Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 21:32:45 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 33 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.901 (L) U 0.901 (L) 5.72 0.901 (L) U 0.541 (L) 8:17 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-13_Form1A_FC3G_013S33_SJ1554641.html; Workgroup: WG42409; Design ID: 1980 ] Page 65 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-13_Form1A_FC3G_013S33_SJ1554641.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF182 Sam ple C ollection: 1 3 -D e c -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-13 M atrix : TISSUE Sam ple Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 21:32:45 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 33 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 33.0 137 24.0 29.5 123 72.0 74.2 103 24.0 24.7 103 24.0 23.0 95.9 24.0 24.2 101 24.0 16.2 67.4 36.0 31.0 86.1 36.0 35.3 98.2 5:11 6:22 7:06 7:29 7:59 9:09 9:59 8:17 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 16.1 134 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-13_Form2_FC3G_013S33_SJ1554641.html; Workgroup: WG42409; Design ID: 1980 ] Page 66 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-13_Form2_FC3G_013S33_SJ1554641.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF054 Sam ple C ollection: 0 7 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-14 (A) M atrix : TISSUE Sam ple Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 21:49:46 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G_013 S: 34 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) U 0.495 (L) 1.08 0.495 (L) 1.31 0.495 (L) 0.773 0.495 (L) U 0.990 (L) U 0.990 (L) 91.7 0.990 (L) 1.52 0.594 (L) 7:59 8:32 9:09 8:17 9:58 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-14_Form1A_FC3G_013S34_SJ1554642.html; Workgroup: WG42409; Design ID: 1980 ] Page 67 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-14_Form1A_FC3G_013S34_SJ1554642.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: CLIENT SAM PLE NO. MSF054 Sam ple C ollection: 0 7 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-14 (A) M atrix : TISSUE Sam ple Size: 2.02 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 21:49:46 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G_013 S: 34 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S V 24.0 36.9 154 24.0 35.2 147 72.0 76.8 107 24.0 29.1 121 24.0 24.5 102 24.0 27.9 116 24.0 20.4 84.9 36.0 37.0 103 36.0 37.2 103 5:12 6:20 7:06 7:29 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFO A 12.0 16.2 135 7:06 (1) Where applicable, custom lab flags have been used on this report; V = surrogate recovery is not within method/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-14_Form2_FC3G_013S34_SJ1554642.html; Workgroup: WG42409; Design ID: 1980 ] Page 68 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-14_Form2_FC3G_013S34_SJ1554642.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. M SF054 (Duplicate) Sam ple C ollection: 0 7 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING WG42409-103 (DUP L19064-14) M atrix : TISSUE Sam ple Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 22:06:47 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 35 Injection V olum e (uL): 15 B lank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) U 0.450 (L) 1.18 0.450 (L) 1.25 0.450 (L) 0.896 0.450 (L) U 0.901 (L) U 0.901 (L) 103 0.901 (L) 1.69 0.541 (L) 7:59 8:32 9:07 8:17 9:58 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-103_Form1A_FC3G_013S35_SJ1554643.html; Workgroup: WG42409; Design ID: 1980 ] Paaggee 69 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-103_Form1A_FC3G_013S35_SJ1554643.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. M SF054 (Duplicate) Sam ple C ollection: 0 7 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING WG42409-103 (DUP L19064-14) M atrix : TISSUE Sam ple Size: 2.22 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 22:06:47 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 35 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G 013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 32.7 136 V 24.0 36.1 150 72.0 77.9 108 24.0 27.7 116 24.0 24.8 103 24.0 25.5 106 24.0 20.6 86.0 36.0 31.2 86.7 36.0 41.3 115 5:10 6:21 7:06 7:29 7:59 9:07 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFO A 12.0 14.4 120 7:06 (1) Where applicable, custom lab flags have been used on this report; V = surrogate recovery is not within method/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-103_Form2_FC3G_013S35_SJ1554643.html; Workgroup: WG42409; Design ID: 1980 ] Page 70 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-103_Form2_FC3G_013S35_SJ1554643.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 PE R FLU O R IN A TED O R G A N IC S A N A LYSIS REPO R T RELATIVE PE RCENT DIFFERENCE A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C ontract No.: 4731 C lient ID: MSF054 C oncentration Units: FALL 2012 TENNESSEE RIVER SAMPLING ng/g (wet weight basis) COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA L19064-14 (A) LAB FLAG 1 CONC. FOUND U U U U U U 1.08 1.31 0.773 U U 91.7 1.52 W G42409-103 LAB FLAG 1 CONC. FOUND U U U U U U 1.18 1.25 0.896 U U 103 1.69 MEAN R E L A T IV E PERCENT DIFFER ENCE 1.13 1.28 0.835 97.3 1.61 8.29 4.95 14.7 11.4 10.2 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: RPD.xsl; Created: 31-Jan-2013 11:32:34; Application: XMLTransformer-1.13.30; ReportFilename: RPD_FC_LC_PFOA-RPD_WG42409-103_L19064-14_.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 o f 1 (WG42409 - RPD_FC_LC_PFOA-RPD_W G42409-103_L19064-14_.html) Page 71 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF064 Sam ple C ollection: 0 7 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-15 M atrix : TISSUE Sam ple Size: 2.51 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 22:23:48 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 36 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.398 (L) U 0.398 (L) U 0.398 (L) U 0.398 (L) U 0.398 (L) U 0.398 (L) 1.38 0.398 (L) 0.606 0.398 (L) 0.700 0.398 (L) U 0.797 (L) U 0.797 (L) 99.8 0.797 (L) 1.46 0.478 (L) 7:59 8:32 9:07 8:17 9:59 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-15_Form1A_FC3G_013S36_SJ1554644.html; Workgroup: WG42409; Design ID: 1980 ] Page 72 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-15_Form1A_FC3G_013S36_SJ1554644.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. MSF064 Sam ple C ollection: 0 7 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-15 M atrix : TISSUE Sam ple Size: 2.51 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 22:23:48 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC 3G _013 S: 36 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG 1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 27.6 115 24.0 31.6 132 72.0 80.1 111 24.0 27.6 115 24.0 21.6 90.2 24.0 9.61 40.1 24.0 8.36 34.8 36.0 21.7 60.4 36.0 35.4 98.5 5:10 6:20 7:06 7:28 7:59 9:07 9:59 8:17 7:17 CLEANUP STANDARD 13C8-PFO A 12.0 15.6 130 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-15_Form2_FC3G_013S36_SJ1554644.html; Workgroup: WG42409; Design ID: 1980 ] Page 73 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-15_Form2_FC3G_013S36_SJ1554644.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF072 Sam ple C ollection: 0 9 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-16 M atrix : TISSUE Sam ple Size: 2.04 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 22:40:41 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 37 Injection V olum e (uL): 15 B lan k Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng/g (wet weight basis) This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.490 (L) U 0.980 (L) U 0.980 (L) 1.66 0.980 (L) U 0.588 (L) 8:17 (1) Where applicable, custom lab flags have been used on this report; U = not detected at RL. (2) Reporting Limit (Code): S = sample detection limit; M = method detection limit; L = lowest calibration level equivalent; Q = contract defined limit. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-16_Form1A_FC3G_013S37_SJ1554645.html; Workgroup: WG42409; Design ID: 1980 ] Page 74 of 234 Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_L19064-16_Form1A_FC3G_013S37_SJ1554645.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Project No. C o ntract No.: 4731 Lab S am ple I.D.: C LIEN T SAM PLE NO. IPF072 Sam ple C ollection: 0 9 -N o v -2 0 1 2 FALL 2012 TENNESSEE RIVER SAMPLING L19064-16 M atrix : TISSUE Sam ple Size: 2.04 g (wet) Sam ple Receipt Date: 08-Jan-2013 Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 22:40:41 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 37 Injection V olum e (uL): 15 Blank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G_013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 28.6 119 24.0 29.7 124 72.0 74.6 104 24.0 26.3 109 24.0 20.8 86.7 24.0 20.8 86.5 24.0 12.8 53.5 36.0 34.2 95.0 36.0 37.7 105 5:11 6:21 7:06 7:28 7:59 9:09 9:58 8:17 7:17 CLEANUP STANDARD 13C8-PFO A 12.0 14.3 119 7:06 (1) Where applicable, custom lab flags have been used on this report. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_L19064-16_Form2_FC3G_013S37_SJ1554645.html; Workgroup: WG42409; Design ID: 1980 ] Page 75 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_L19064-16_Form2_FC3G_013S37_SJ1554645.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 1A PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO RT A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 C o ntract No.: 4731 Project No. Lab S am ple I.D.: C LIEN T SAM PLE NO. Lab Blank Sam ple C ollection: N/A N/A W G42409-101 M atrix : AQ U EO US (TISSUE M ETHOD) Sam ple Size: 2.00 g Sam ple Receipt Date: N/A Initial C alibration Date: 1 1 -Jan-2 013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 17:52:20 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 20 Injection V olum e (uL): 15 B lan k Data Filenam e: FC 3G _013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC 3G _013 S: 14 C oncentration Units: ng/g This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. COMPOUND LAB FLAG 1 CONC. FOUND REPO RTING LIM IT (RL)2 R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA U U U U U U U U U U U U U 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 0.500 (L) 1.00 (L) 1.00 (L) 1.00 (L) 0.600 (L) (1) W here applicable, custom lab flags have been used on this report; U = not detected at RL. (2) R eporting Lim it (C ode): S = sam ple detection limit; M = m ethod detection limit; L = low est calibration level equivalent; Q = con tract defined limit. T h e s e da ta are v a lid a te d an d re p o rte d a s a c c u ra te an d in a cco rd w ith A X Y S A n a ly tic a l S e rv ic e s Ltd. IS O 1 7 0 2 5 co m p lia n t q u a lity a s s u ra n c e p ro ce sse s. S ig n e d :_____________ Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form1A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-101_Form1A_FC3G_013S20_SJ1554627.html; Workgroup: WG42409; Design ID: 1980 ] Paaggee 76 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-101_Form1A_FC3G_013S20_SJ1554627.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 2 PE R FLU O R IN A TED O R G A N IC S A N A LYS IS REPO R T A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 C o ntract No.: 4731 Project No. Lab S am ple I.D.: C LIEN T SAM PLE NO. Lab Blank Sam ple C ollection: N/A N/A WG42409-101 M atrix : AQUEOUS (TISSUE METHOD) Sam ple Size: 2.00 g Sam ple Receipt Date: N/A Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 Tim e: 17:52:20 C olum n ID: C18MS Extract V olum e (uL): 4000 Sam ple Data Filenam e: FC3G _013 S: 20 Injection V olum e (uL): 15 B lank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G 013 S: 14 C oncentration Units: ng absolute This page is part of a total report that contains information necessary for accreditation compliance. Results are compliant with NELAP accreditation described in the total report. Sample results relate only to the sample tested. LABELED COMPOUND LAB FLAG1 S P IK E CONC. CONC. FOUND R(%) 2 R E T E N T IO N TIM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S V 24.0 38.7 161 24.0 35.7 149 72.0 81.4 113 24.0 26.1 109 24.0 18.7 78.1 24.0 18.2 75.7 24.0 20.8 86.5 36.0 42.3 117 36.0 42.7 119 5:16 6:21 7:06 7:29 8:03 9:11 9:59 8:21 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 13.0 109 7:06 (1) Where applicable, custom lab flags have been used on this report; V = surrogate recovery is not within method/contract control limits. (2) R(%) = percent recovery. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ For Axys Internal Use Only [ XSL Template: FC-Form2.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-101_Form2_FC3G_013S20_SJ1554627.html; Workgroup: WG42409; Design ID: 1980 ] Page 77 of 234 Page 1 of 1 (WG42409-PFC_FC_LC_PFOA_WG42409-101_Form2_FC3G_013S20_SJ1554627.html) www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 8A PERFLUO RINATED O RG ANICS O NG O ING PRECISIO N AND R EC O VERY (OPR) A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 C o ntract No.: 4731 Lab S am ple I.D.: WG42409-102 M atrix : TISSUE Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 17:01:25 C olum n ID: C18MS Extract V olum e (uL): 4000 O P R D ata Filenam e: F C 3 G _ 0 1 3 S: 17 Injection V olum e (uL): 15 B lank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G 013 S: 14 A L L C O N C E N T R A T IO N S R E P O R TE D O N TH IS FO R M A R E C O N C E N T R A T IO N S IN E X TR A C T , B A S E D O N A 1 m L E X T R A C T V O L U M E . COMPOUND LAB FLAG 1 SPIKE CONC. (ng/m L) CONC. FOUND (n g /m L ) % RECOVERY R E T E N T IO N TIM E PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 40.0 34.8 40.0 31.4 40.0 36.0 40.0 31.6 40.0 34.8 40.0 31.1 40.0 34.0 40.0 33.0 40.0 39.9 80.0 78.5 80.0 74.5 80.0 65.6 40.0 34.8 87.0 78.5 90.0 79.1 86.9 77.7 85.0 82.6 99.8 98.1 93.1 82.0 86.9 5:14 5:58 6:22 6:44 7:09 7:33 8:03 8:35 9:13 6:29 7:20 8:21 9:59 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-102_Form8A_SJ1554623.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_WG42409-102_Form8A_SJ1554623.html) Page 78 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 8B PERFLUORINATED ORGANICS ONGOING PRECISION AND RECOVERY (OPR) A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 C ontract No.: 4731 Lab S am ple I.D.: WG42409-102 M atrix : TISSUE Initial C alibration Date: 11-Jan-2013 Extraction Date: 10-Jan-2013 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 T im e: 17:01:25 C olum n ID: C18MS Extract V olum e (uL): 4000 O P R D ata Filenam e: F C 3 G _ 0 1 3 S: 17 Injection V olum e (uL): 15 B lank Data Filenam e: FC3G_013 S: 20 D ilution Factor: N/A Cal. Ver. Data Filenam e: FC3G 013 S: 14 A L L C O N C E N T R A T IO N S R E P O R TE D O N TH IS FO R M A R E C O N C E N T R A T IO N S IN E X TR A C T , B A S E D O N A 1 m L E X T R A C T V O L U M E . LABELED COMPOUND LAB FLAG 1 SPIKE CO N C . (ng/m L) CONC. FOUND (ng/m L) % RECOVERY R E T E N T IO N T IM E 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 24.0 29.4 122 24.0 30.4 126 72.0 88.1 122 24.0 31.9 133 24.0 26.2 109 24.0 23.9 99.4 24.0 26.0 108 36.0 44.2 123 36.0 39.7 110 5:11 6:22 7:06 7:33 8:03 9:13 9:59 8:21 7:20 CLEANUP STANDARD 13C8-PFO A 12.0 15.1 126 7:06 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Kristina Coleman______ These pages are part of a larger report that may contain information necessary for full data evaluation. Results reported relate only to the sample tested. For Axys Internal Use Only [ XSL Template: FC-Form8B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFC_FC_LC_PFOA_WG42409-102_Form8B_SJ1554623.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFC_FC_LC_PFOA_WG42409-102_Form8B_SJ1554623.html) Page 79 of 234 www.axysanalytical.com ____________ Fit Sample List Report PPo J: v^g-e,________________ .________________ MassLynx 4.1 SCN627 Sample List: G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC3G_013.SPL Last Modified: Friday, January 11, 2013 13:01:07 Pacific Standard Time Printed: Friday, January 11, 2013 13:05:15 Pacific Standard Time Page 1 of 1 Page Position (1,1) File Name Sample ID File Text Bottle Signln SignOut Inj VoL 1 FC3G 013S001 MeOH,, 2 FC3G 013S002 MeOH,, 1,,15uL 1,,15uL L IU 1:8,A - 1:8,A - - iM "1- 15.0 15.0 3 FC3G 013S003 FC-LIN-42151T-A,,1/2 1,,15uL 2:1,A - - 15.0 4 FG3G 013S004 FC-LIN-42151T-A,,1/2 1,,15uL 2:1,A - - . - - 15.0 -, 5 FC3G 013S005 FC-LiN-42151T-B,,1/2 1,,15uL 2:1,B -- - -- 15.0 6 FC3G 013S006 FC-LIN-42151T-e,,1/2 1,,15uL 2:1 ,C - - --- 15.0 7 FC3G 013S007 FC-LIN-42151T-D,, 1/2 1,,15uL 2:1,D - - --- 15.0 V 8 FC3G 013S008 FC-LIN-42151T-E,, 1/2 1,,15uL 2:1,E - - -- 15.0 9 FC3G 013S009 FC-LIN-42151T-F..1/2 1,,15uL 2:1,F - - - 15.0 10 FC3G 013S010 FC-LIN-42151T-G,, 1/2 1,,15uL 2:2,A - - -- 15.0 11 FC3G 013S011 FC-LIN-42151T-H,,1/2 1,,15uL 2:2,B - -- 15.0 J 12 FC3G 013S012 MeOH,, 1,,15uL 1:8,A - -- 15.0 13 FC3G O13S013 MeOH,, 1,,15uL 14 FC3G 013S014 FC-LIN-42151T-D,,1/2 1,,15uL 15 FC3G 013S015 FC-LIN-42151T-D,,1/2 1,, 15uL 1:8,A - 1 15,0 2:1,D - "t / 15.0 m 2:1,D - 15.0 16 FC3G 013S016 WG42409-104,,SAR 1 ,WG42409,15/4000uL 3:4,A - 15.0 17 FC3G 013S017 WG42409-102,,SPM 1,WG42409,15/4000uL 3:4, B ' - - 15.0 18 FC3G 013S018 MeOH,, 1,,15uL 1:8,A - - 15.0 19 FC3G 013S019 MeOH,, 20 FC3G 013S020 WG42409-101,,BLK 1,,15uL ' 1:8,A 1.WG42409,15/4000uL 3:4,C - - 15.0 15.0 21 FC3G 013S021 L19064-1,, 1,WG42409,15/4000uL 3:4,D - - 15.0 22 FC3G 013S022 L19064-2,,. 1,WG42409,15/4000uL 3:4,E - - 15.0 23 FC3G 013S023 L19064-3,, 24 FC3G 013S024 L19064-4,, 1,WG42409,15/4000uL 3:4,F - 1,WG42409,15/4000uL 3:5,A - - - 15.0 15.0 25 FC3G 013S025 L19064-5,, 26 FC3G 013S026 L19064-6,, 1,WG42409,15/4000uL 3:5,B 1,WG42409,15/4000uL .3:5,C - -- - 15.0 15.0 27 FC3G 013S027 L19064-7,,. 1,WG42409,15/4000uL 3:5,D - - - 15.0 28 FC3G 013S028 L19064-8,, 1,WG42409,15/4000uL 3:5,E - - -- 15.0 29 FC3G 013S029 L19064-9,, 1,WG42409,15/4000uL 3:5,F - - . - 15.0 30 FC3G 013S030 L19064-10,, 31 FC3G 013S031 L19064-11 ,, 1,WG42409,15/4000uL 3:6,A - 1,WG42409,15/4000uL 3:6,B - - * -- 15.0 15.0 32 FC3G 013S032 L19064-12,, 33 FC3G 013S033 L1964-13,, 34 FC3G 013S034 L19064-14,, 1,WG42409,15/4000uL 3:6,C 1,WG42409,15/4000uL 3:6,D 1.WG42409,15/4000UL 3:6,E ---- -- -- -- 15.0 15.0 15.0 35 FC3G 013S035 WG42409-103,,DUP 1,WG42409,15/4000uL 3:6,F - - 15.0 36 FC3G 013S036 L19064-15,, 1,WG42409,15/4000uL 3:7,A - - -- 15.0 37 FC3G 013S037 L19064-16,, 1,WG42409,15/4000uL 3:7,B --- 15.0 38 FC3G 013S038 FC-LIN-42151T-D,,1/2 1,,15uL 39 FC3G 013S039 FC-LIN-42151T-D,,1/2 1,,15uL 40 FC3G 013S040 MeOH,, 1,,15uL 2:1,D 2:1,D 1:8,A -f / 15.0 V7 15.0 15.0 A( O c{c- l i r > L-70-S A cA ^ ls C X v^l'iJp le . i2T A K 2oL 2 Page 80 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 3A PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES AXYS ANA LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 11 -Jan-2013 CS0 D ata Filenam e: CS1 D ata Filenam e: FC3G_013 S: 4 FC3G_013 S: 5 In stru m en t ID: LC MS/MS CS2 D ata Filenam e: FC3G_013 S: 6 LC C o lum n ID: C18MS CS3 D ata Filenam e: FC3G_013 S: 7 C S4 D ata Filenam e: FC3G_013 S: 8 CS5 D ata Filenam e: FC3G_013 S: 9 CS6 D ata Filenam e: FC3G_013 S: 10 CS7 D ata Filenam e: FC3G_013 S: 11 CS8 D ata Filenam e: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLAG1 CS0 CS1 CS2 PER C EN T R EC O V ER Y (%) CS3 CS4 CS5 CS6 CS7 CS8 102 99.4 84.6 98.7 110 106 106 93.4 100 105 83.0 88.7 100 116 111 96.2 98.7 106 88.8 101 107 110 104 84.0 102 99.9 84.3 101 99.7 117 110 86.9 97.9 107 92.3 95.1 110 104 105 89.5 98.2 105 97.7 93.0 111 95.9 103 95.0 93.0 119 95.0 98.6 106 101 87.0 94.0 117 89.4 101 113 110 104 71.2 92.0 119 103 110 116 110 89.2 61.7 100 102 90.3 95.4 106 111 102 93.5 102 97.9 89.4 93.4 107 110 108 92.7 98.2 108 84.8 99.6 103 98.0 114 94.9 102 98.6 87.2 97.1 104 104 105 102 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3A_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409-PFOA_FC_LC_11-Jan-2013_FC3G__Form3A_GS48661.html) Page 81 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 3B PERFLUORINATED ORGANICS INITIAL CALIBRATION PERCENT RECOVERIES AXYS ANA LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 11 -Jan-2013 CS0 D ata Filenam e: CS1 D ata Filenam e: FC3G_013 S: 4 FC3G_013 S: 5 In stru m en t ID: LC MS/MS CS2 D ata Filenam e: FC3G_013 S: 6 LC C o lum n ID: C18MS CS3 D ata Filenam e: FC3G_013 S: 7 C S4 D ata Filenam e: FC3G_013 S: 8 CS5 D ata Filenam e: FC3G_013 S: 9 CS6 D ata Filenam e: FC3G_013 S: 10 CS7 D ata Filenam e: FC3G_013 S: 11 CS8 D ata Filenam e: N/A LABELED COMPOUND 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 1 8 O 2-P F H xS CLEAN-UP STANDARD 13C8-PFO A LAB FLAG1 CS0 87.3 99.1 93.8 93.7 86.8 88.4 85.7 96.7 104 104 CS1 96.2 103 98.1 101 76.9 88.2 81.4 97.1 113 94.3 CS2 PER C EN T REC O VER IES (%) CS3 CS4 CS5 CS6 102 82.5 103 115 116 109 91.7 99.2 96.5 97.2 101 95.8 100 103 102 94.3 90.1 98.4 112 108 87.7 76.2 95.6 105 123 89.8 85.6 115 133 97.0 84.8 121 130 97.3 83.3 103 115 106 110 99.0 103 94.9 92.5 101 93.1 102 103 101 CS7 97.9 105 106 103 149 102 83.9 102 CS8 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3B_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_11-Jan-2013_FC3G__Form3B_GS48661.html) Page 82 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 3C PERFLUORINATED ORGANICS INITIAL CALIBRATION RETENTION TIMES AXYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 11 -Jan-2013 CS0 D ata Filenam e: CS1 D ata Filenam e: FC3G_013 S: 4 FC3G_013 S: 5 Instrum ent ID: LC MS/MS CS2 D ata Filenam e: FC3G_013 S: 6 LC C olum n ID: C18MS CS3 Data Filenam e: FC3G_013 S: 7 C S4 Data Filenam e: FC3G_013 S: 8 CS5 D ata Filenam e: FC3G_013 S: 9 CS6 D ata Filenam e: FC3G_013 S: 10 CS7 D ata Filenam e: FC3G_013 S: 11 CS8 Data Filenam e: N/A COMPOUND PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA LAB FLAG1 CS0 CS1 CS2 RETEN TIO N TIM ES CS3 CS4 CS5 CS6 CS7 CS8 MEAN RT 5:15 5:15 5:16 5:16 5:15 5:15 5:12 5:16 5:59 6:01 5:59 5:59 5:58 5:58 5:57 5:58 6:23 6:23 6:23 6:22 6:21 6:22 6:21 6:21 6:44 6:47 6:44 6:44 6:44 6:47 6:44 6:44 7:09 7:09 7:09 7:09 7:09 7:09 7:09 7:06 7:36 7:36 7:33 7:33 7:33 7:33 7:33 7:29 8:07 8:07 8:03 8:03 8:03 8:03 8:03 8:40 8:42 8:39 8:37 8:37 8:39 8:37 8:37 9:16 9:18 9:16 9:15 9:15 9:16 9:15 9:15 6:32 6:32 6:31 6:31 6:30 6:31 6:30 6:29 7:23 7:23 7:20 7:23 7:23 7:23 7:20 7:20 8:26 8:26 8:24 8:24 8:21 8:24 8:21 8:21 9:58 9:59 9:59 9:58 9:59 9:59 9:59 9:59 5:15 5:59 6:22 6:45 7:09 7:33 8:04 8:39 9:16 6:31 7:22 8:23 9:59 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3C.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3C_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409-PFOA_FC_LC_n-Jan-2013_FC3G_Form3C_GS48661.html) Page 83 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 3D PERFLUORINATED ORGANICS INITIAL CALIBRATION RETENTION TIMES AXYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 11 -Jan-2013 CS0 D ata Filenam e: CS1 D ata Filenam e: FC3G_013 S: 4 FC3G_013 S: 5 Instrum ent ID: LC MS/MS CS2 D ata Filenam e: FC3G_013 S: 6 LC C olum n ID: C18MS CS3 Data Filenam e: FC3G_013 S: 7 C S4 Data Filenam e: FC3G_013 S: 8 CS5 D ata Filenam e: FC3G_013 S: 9 CS6 D ata Filenam e: FC3G_013 S: 10 CS7 D ata Filenam e: FC3G_013 S: 11 CS8 Data Filenam e: N/A LABELED COMPOUND 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 1 3 C 2 -P F O A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 13C4-PFO S 18 O 2-P F H x S LAB FLAG1 CS0 CS1 CS2 RETEN TIO N TIM ES CS3 CS4 CS5 CS6 CS7 CS8 MEAN RT 5:15 5:15 5:17 5:15 5:16 5:16 5:14 5:15 6:22 6:23 6:22 6:22 6:21 6:22 6:21 6:20 7:09 7:09 7:09 7:09 7:09 7:09 7:06 7:06 7:33 7:36 7:33 7:33 7:33 7:33 7:33 7:29 8:07 8:07 8:03 8:03 8:03 8:03 8:03 8:03 9:16 9:18 9:15 9:15 9:15 9:15 9:58 9:59 9:58 9:58 9:58 9:59 8:24 8:26 8:24 8:24 8:24 8:24 8:21 8:24 7:23 7:23 7:23 7:23 7:20 7:23 7:20 7:20 5:15 6:22 7:08 7:33 8:04 9:16 9:58 8:24 7:22 CLEAN-UP STANDARD 1 3 C 8 -P F O A 7:09 7:09 7:09 7:09 7:09 7:09 7:06 7:06 7:08 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Andrew Porat______ For Axys Internal Use Only [ XSL Template: FC-Form3D.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_11-Jan-2013_FC3G__Form3D_GS48661.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_11-Jan-2013_FC3G__Form3D_GS48661.html) Page 84 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 11-Jan-2013 V E R Data Filenam e: FC3G_013 S: 14 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 LC C o lum n ID: C18MS A n alysis Tim e: 16:10:30 COMPOUND LAB FLAG1 R ETEN TIO N TIM E EXPECTED CONC. (ng) C O N C .FO U N D (ng) RECOVERY PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 5:14 40.0 37.6 94.0 5:58 40.0 36.0 90.0 6:22 40.0 40.7 102 6:44 40.0 38.8 96.9 7:06 40.0 39.1 97.8 7:29 40.0 37.1 92.6 8:03 40.0 41.2 103 8:35 40.0 41.2 103 9:13 40.0 45.0 113 6:30 80.0 85.3 107 7:20 80.0 84.3 105 8:21 80.0 80.7 101 9:59 40.0 40.5 101 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S14__Form4A_SJ1554620.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S14__Form4A_SJ1554620.html) Page 85 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 11-Jan-2013 V E R Data Filenam e: FC3G_013 S: 14 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 LC C o lum n ID: C18MS A n alysis Tim e: 16:10:30 LABELED COMPOUND LAB FLAG 1 RETEN TIO N TIM E EXPECTED CONC. (ng) C O N C .FO U N D (ng) R E C O V ER Y (%) 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S 5:15 24.0 23.1 96.4 6:22 24.0 25.8 108 7:06 72.0 78.0 108 7:29 24.0 25.7 107 8:03 24.0 21.9 91.2 9:13 24.0 22.5 93.7 9:59 24.0 25.1 104 8:21 36.0 35.4 98.3 7:20 36.0 36.5 101 CLEANUP STANDARD 13C8-PFO A 7:06 12.0 13.4 112 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S14__Form4B_SJ1554620.html; Workgroup: WG42409; DesignID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S14__Form4B_SJ1554620.html) Page 86 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4A PERFLUORINATED ORGANICS CALIBRATION VERIFICATION A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 11-Jan-2013 V E R Data Filenam e: FC3G_013 S: 38 Instru m en t ID: LC MS/MS A nalysis Date: 11-Jan-2013 LC C o lum n ID: C18MS A n alysis Tim e: 22:57:42 COMPOUND LAB FLAG1 R ETEN TIO N TIM E EXPECTED CONC. (ng) C O N C .FO U N D (ng) RECOVERY PFBA PFPeA PFHxA PFHpA PFOA PFNA PFDA PFUnA PFDoA PFBS PFHxS PFOS PFOSA 5:11 40.0 37.3 93.2 5:57 40.0 35.3 88.2 6:20 40.0 40.9 102 6:41 40.0 36.8 92.0 7:06 40.0 38.8 97.0 7:28 40.0 37.8 94.4 7:59 40.0 40.0 100 8:33 40.0 42.1 105 9:09 40.0 45.5 114 6:29 80.0 86.3 108 7:17 80.0 81.1 101 8:17 80.0 75.6 94.5 9:58 40.0 41.3 103 (1) Where applicable, custom lab flags have been used on this report. These data are validated and reported as accurate and in accord with AXYS Analytical Services Ltd. ISO17025 compliant quality assurance processes. Signed:___________Leanne Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4A.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S38_Form4A_SJ1554646.html; Workgroup: WG42409; Design ID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S38__Form4A_SJ1554646.html) Page 87 of 234 www.axysanalytical.com AXYS METHOD MLA-043 Rev 8 Form 4B PERFLUORINATED ORGANICS CALIBRATION VERIFICATION A XYS A N A LYTIC A L SERVICES 2045 MILLS RD., SIDNEY, B.C., CANADA V8L 5X2 TEL (250) 655-5800 FAX (250) 655-5811 Initial C alibration Date: 1 1 -Jan-2 013 V E R Data Filenam e: FC 3G _013 S: 38 Instru m en t ID: LC MS/M S A nalysis Date: 1 1 -Ja n -2 0 1 3 LC C o lum n ID: C18MS A n alysis Tim e: 22:57:42 LABELED COMPOUND 1 3 C 4 -P F B A 1 3 C 2 -P F H x A 13C2-PFO A 1 3 C 5 -P F N A 1 3 C 2 -P F D A 1 3 C 2 -P F D o A 13C8-PFO SA 1 3 C 4-P F O S 18 O 2-P F H x S CLEANUP STANDARD 13C8-PFO A LAB FLAG 1 RETEN TIO N TIM E 5:11 6:20 7:03 7:28 7:59 9:09 9:59 8:17 7:17 EXPECTED CONC. (ng) 24.0 24.0 72.0 24.0 24.0 24.0 24.0 36.0 36.0 C O N C .FO U N D (ng) 23.3 24.8 72.0 23.0 20.3 18.4 19.8 31.0 31.8 R E C O V ER Y (%) 97.1 103 100 95.7 84.7 76.8 82.5 86.2 88.4 7:03 12.0 12.5 104 (1) W here applicable, custom lab flags have been used on this report. T h e s e da ta are v a lid a te d an d re p o rte d a s a c c u ra te an d in a cco rd w ith A X Y S A n a ly tic a l S e rv ic e s Ltd. IS O 1 7 0 2 5 co m p lia n t q u a lity a s s u ra n c e p ro ce sse s. LeanneS ig n e d :_____________ Henley______ For Axys Internal Use Only [ XSL Template: FC-Form4B.xsl; Created: 31-Jan-2013 11:31:33; Application: XMLTransformer-1.13.30; ReportFilename: PFOA_FC_LC_FC3G_013S38_Form4B_SJ1554646.html; Workgroup: WG42409; DesignID: 1980 ] Page 1 of 1 (WG42409 - PFOA_FC_LC_FC3G_013S38__Form4B_SJ1554646.html) Page 88 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-1,, Name: FC3G_013S021 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:09:13 PFBA FC3G_013S021 100- 6.23 (A ,,6.57 %,, /4/.26 I I Vyisv, 5.00 6.00 7.00 PFPeA FC3G013S021 100- 6.68 6.21 \ \ 5.95. 7.16 % .-4.33 M W J / \! 5.00 6.00 7.00 PFHxA FC3G_013S021 100- 6.38 7.78e2 3442 %- 6.16 [ 4f i 's A"-' 5.00 6.00 7.00 PFNA FC3G013S021 7 nn 1 0 0 -1, 4.38e.1258 7.05 T. _____ 7.00 V .r-T-r-i-i min 8.00 PFDA FC3G 013S021 100 *, 7.71e3 iI 28852 % !, n 0 jL - '-kTM , - _____- min 8.00 10.00 12.00 PFUnA FC3G013S021 i h . 8.58 .02e4 16666 %" 0 8.00 10.00 12.00 PFHxS-1 FC3G 013S021 7.29;4.71e1;194 100n 6.86 / \ 7n90 A%- J . I ,----- , -------- , | r. -T min 7.00 8.00 PFHxS-2 FC3G__013S021 100" , 7.38 A %- I \ / >7.72 8 ^ 0 I I /fA I , i X-r.i.m - n min 7.00 8.00 PFHxS-3 FC3G_013S021 100" 6.63 7.46 7.78 - %- 0J 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S021 100- 8.71e2~l 2981 0 38 5'6b ' ' L~------------------- mi n F00 PFOA-1 FC3G 013S021 10 0 - C 6.59e2 / \ 2417 %- - 07.00 . ''84 r~Z min 8.00 PFOA-2 FC3G 013S021 10 0 n %2 6.63/ \ . 07.00 8.00 PFDoA FC3GJD13S021 100- 9.18 F 7.94e3 I 36027 % 8.00 10.00 12.00 PFBS-1 FC3G_013S021 100- 6.73 5.00,.5.79 5.97 ! 7.11 / i/i ft 1A Jvli % 5.00 6.00 7.00 PFBS-2 FC3G 013S021 PFOS-1 FC3G 013S021 8.29 1 0 0 3.87e4 : ; 136065 % I ;5; 0- \ 8.00 10.00 min 12.00 PFOS-2 FC3G_013S021 1 0 0 -q 8.29 3.22e4 ; 117484 %- 0 -* min 8.00 10.00 12.00 PFOSA FC3G_013S021 100 9.99 2.63e2 1421 %- O 8.00 10.00 12.00 m Page 89 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PR0\FC\FC3G..013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-1,, Name: FC3G_013S021 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:09:13 13C2-PFOA FC3G 013S021 100-i - 7.09 ^2.4265 963090 %- 13C8-PFOA FC3G 013S021 100- 7.09 k 8.86e4 352166 13C4-PFOA FC3G 013S021 100- - 7.09 `~9.47e4 373906 %- o: r v j --------- .'I i '! -rr-nTinmin 7.00 8.00 u0 -- ;----7.00 j. ,t, -7 min 8.00 u0 -- f n - p-T 7.00 i 8.00 1802-PFHxS-2 FC3GJJ13S021 100 n 7.*20e3 28931 -- --------V t-tt min 7.00 8.00 13C4-PFBA FC3G 013S021 1 0 0 -- 2.32e4 85108 %4.07 0J 111` 1 1 1 1`^fiM*TfrffrmTiTTTT min 5.00 6.00 7.00 13C2-PFHxA FC3G013S021 100-1 luu - ; 6.34 6.36e4 1 296988 I %- 05.00 6.00 7.00 13C8-PFOSA FC3G_013S021 100- 9.96 9.92e3~] 56773 %- 13C2-PFOUEA FC3G_013S021 1IU0U0n- 2.79'43e84 - l l 3722 ro 8.00 10.00 12.00 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S021 100- 8.35 f 9.38e3 : 33424 13C4-PFOS-2 FC3G 013S021 100q 8.29 i" 7.76e3 I 29588 1802-PFHxS-1 FC3G 013S021 100 7.33 1.06e4 ! 43262 ; o : min 8.00 10.00 12.00 (H ...... min 8.00 10.00 12.00 07.00 8.00 13C5-PFNA FC3G_013S021 100 7.49 8.85o4 . 36104/ %- 13C2-PFDA FC3G 013S021 100" 7.99 9.64e4 364666 1302-PFDoA FC3G_013S021 100- 9.18 f 1.37e5 0 575308 %- 7.00 8.00 8.00 10.00 12.00 8.00 10.00 12.00 m Page 90 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday. January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-1,, Name: FC3G_013S021 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:09:13 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.19 27 262.98 >218.... 27 312.97> 268.98 6.38 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.55 29 512.96 > 468.... 7.99 29 562.96 >518.... 8.58 30 612.95 > 568.... 9.18 24 298.94> 79.96 6.47 25 298.94 > 98.95 24 398.84 > 79.96 7.29 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 37 1.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.29 21 216.99>171.98 5.23 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.18 21 506>78 9.96 458.98 >393.98 7.38 Area 591 778 871 659 158 438 7713 10168 7942 13 47 38673 32211 263 241523 88625 94653 9384 7759 10612 7204 23238 63631 88521 96450 136709 9918 29407 IS Area 23238 63631 63631 63631 241523 241523 88521 96450 96450 136709 10612 7204 10612 7204 10612 9384 7759 9918 94653 94653 94653 94653 94653 94653 94653 94653 94653 94653 94653 94653 ng/g 0.042 0.058 0.540 0.721 0.423 0.034 56.560 68.149 0.129 39.025 7.314 5.959 15.133 15.347 17.440 17.800 11.698 13.401 13.881 11.314 9.717 7.531 5.074 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0602 0.0342 0.0193 0.0062 0.0171 0.0778 0.1348 0.0405 0.1601 0.0185 0.0314 0.0466 0.0221 0.0557 0.0235 %Rec S/N 7 10 8 35 4 3 130 153 198 5 3 111.1 1.24.9 101.8 86.2 87.4 99.3 101.4 99.9 114.5 118.6 96.6 83.0 64.3 104.0 804 2041 71 6125 3532 1047 526 330 1332 0 203 2563 1368 697 1402 588 648 Noise 279 199 336 354 69 216 420 223 305 182 24 28 64 38 54 169 58 20 157 100 357 64 90 32 0 419 116 264 523 410 97 176 Height 1924 3442 2981 2417 830 1258 28852 46666 36027 117 194 136065 117484 . 1421 963090 352166 373906 33424 29588 43262 28931 85108 296988 361042 364666 575308 56773 113722 IS Height Sample size 85108 2.0500 296988 2.0500 296988 2.0500 296988 2.0500 963090 2.0500 963090 2,0500 361042 2.0500 364666 2.0500 364666 2.0500 575308 2.0500 43262 2.0500 28931 2.0500 43262 2.0500 28931 2.0500 43262 2.0500 33424 2.0500 29588 2.0500 56773 2.0500 373906 2.0500 373906 2.0500 2.0500 373906 2.0500 373906 2.0500 373906 2.0500 373906 2.0500 373906 2.0500 373906 2.0500 373906 2.0500 373906 373906 2.0500 2.0500 373906 2.0500 2.0500 Factor2 Detection... 1.0 MMI 1.0 1.0 bbl 1.0 bb 1.0 MMI 1.0 MM 1.0 bbl 1.0 bb 1.0 MM 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 91 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\MasslynxMnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-2,, Name: FC3G_013S022 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:26:14 PFBA FC3G 013S022 100- % - 4.07 / i,(\v 6.40 J! Vvi1i l\ H r 6 -55 i 0 - T,^ m ;n 5.00 6.00 7.00 PFPeA FC3G_013S022 100-1 5.90 _ 2.04e2 1,6-53 ; 951 4 52 %- f|S ,! \ fA ( "'<r' \ i X !v r o: 5.00 6.00 7.00 PFNA FC3G_013S022 100-, 7.46 4.47e2 1935 % %--6.63 \ 8.02 PFDA FC3G 013S022 PFHxA FC3G 013S022 100- 6.38 1.23e3 | 5158 1 % - 6.06 | \ 7.26 05.00 6.00 7.00 PFUnA FC3G013S022 100n 8.58 1.56e4 l 69234 7.00 8.00 8.00 10.00 12.00 PFHxS-1 FC3G_013S022 fi TK 100- A 7.24 7.49 : > /\ \ / V\ :V \ \ 7^ ~r mi n 7.00 8.00 PFHxS-2 FC3G 013S022 oo .J i_ i_j_ \ \ %- r /1 7 8 r\ \ o- ^ \ . J -n-r-r min 7.00 8.00 PFHxS-3 FC3G__013S022 100^1 7.19 a \ 7.38 ( %- A o: 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S022 PFDoA FC3G_013S022 100- 9.18 I 3.41 e4 I 143698 I %- PFOA-1 FC3G_013S022 100- 7.09 . 9.70e2 \ 3441 %- \ 7.00 8.00 PFBS-1 FC3G 013S022 PFOA-2 FC3G 013S022 PFBS-2 FC3G013S022 8.00 10.00 12.00 PFOS-1 FC3G 013S022 100- 8.35 T 2.57e5 1 862328 fi 8 o- i/ V : 8.00 10.00 12.00 PFOS-2 FC3G_013S022 100- 8.35 T 2.01 e5 I 719924 %- ia a . 8.00 10.00 12.00 PFOSA FC3G_013S022 100- 9.99 4.22 e3 I 23155 1 %- 8.00 10.00 12.00 m Page 92 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Massiynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-2,, Name: FC3G_013S022 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:26:14 13C2-PFOA FC3G 013S022 100-i . 7.09 2.25e5 912986 13C8-PFOA FC3G 013S022 100- 7.09 K 8.98e4 364964 13C4-PFOA FC3G_013S022 100-1 7.09 9.22e4 362217 07.00 -t-7 min 8.00 0 illlitik , l min 7.00 8.00 7.00 oo 1802-PFHxS-2 FC3G 013S022 7.33 6.89e3 ' 27840 %- o- TT 7.00 -- min 8.00 13C4-PFBA FC3G 013S022 100- 5.23 2.25e4 87551 %- 4.03 / 0- 5.00 6.00 min 7.00 13C2-PFHxA FC3G 013S022 100- 6.36 6.37e4 i\ 275500 %- 0 - ........... -m 5.00 6.00 7.00 13C8-PFOSA FC3G013S022 inn 996 10 n 1 .0 5 e 4 l 56781 I %- 13C2-PFOUEA FC3G_013S022 100-- 7.38 3.32e4 ""Jl 118435, , %- 8.00 10.00 12.00 n min 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S022 100- 8.35 F 7.96e3 28219 %" ! 1 o- . min 8.00 10.00 12.00 13C5-PFNA FC3G 013S022 100n 7.49 8.31c4 . 321033 m %- / ------------------- ^ 7.00 8.00 min 13C4-PFOS-2 FC3G 013S022 100- 8.35 5 6.82.03 ; 24826 % j, ; 0 8.00 -- ----------T-P'T min 10.00 12.00 13C2-PFDA FC3G 013S022 100- 7.99 f 8.73e4 1 316044 %m m o-: ite .: 8.00 10.00 12.00 1802-PFHXS-1 FC3G_013S022 100- 7.33 9.69e3"4 39272 / i % 0 7.00 8.00 13C2-PFDOA FC3GJ513S022 100q 9.18 I 1.44e5 638573 %- 8.00 10.00 12.00 m Page 93 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-2,, Name: FC3G_013S022 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:26:14 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 1 0 PFDoA 1 1 PFBS-1 1 2 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 2 0 13C8-PFOA 2 1 13C4-PFOA 2 2 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 1302-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.30 27 262.98 >218. .. 5.90 27 312.97> 268.98 6.38 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 > 418.... 7.46 29 512.96 > 468.... 7.99 29 562.96 >518.... 8.58 30 612.95 >568.... 9.18 24 298.94> 79.96 25 298.94 > 98.95 6.47 24 398.84 > 79.96 25 398.84> 98.96 24 398.84> 118.70 2 2 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 2 1 414.97 >369.... 7.09 2 1 420.97 > 375.... 7.09 416.97 >371.9 7.09 2 1 503 > 79.96 8.35 2 1 503 > 98.96 2 1 402.9>84 2 1 402.9>103 8.35 7.33 7.33 2 1 216.99>171.98 5.23 2 1 314.97>269.98 6.36 2 1 467.96>422.97 7.49 2 1 514.96 >469.96 7.99 2 1 614.95 > 569.... 9.18 2 1 506>78 9.96 458.98 >393.98 7.38 Area 451 204 1233 1095 970 162 447 17717 15555 34059 7 256642 200842 4217 224963 89822 92247 7958 6820 9691 6891 22530 63714 83107 87295 143692 10541 33225 IS Area 22530 63714 63714 63714 224963 224963 83107 87295 87295 143692 9691 6891 9691 6891 9691 7958 6820 10541 92247 92247 92247 92247 92247 92247 92247 92247 92247 92247 92247 92247 ng/g 0.032 0.069 0.026 0.065 1.674 1.261 2.716 432.137 472.428 4.070 36.236 7.389 5.643 12.793 13.447 15.877 16.975 11.306 13.377 12.991 10.209 10.182 7.979 5.569 LOD 0.0413 0.1422 0.0266 0.0054 0.0066 0.0905 0.1504 0.1213 0.3635 0.2824 0.0207 0.0181 0.0194 0.0190 0.1254 0.0076 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 5 3 18 11 10 0 5 143 307 415 4 106.2 129.9 99.2 75.0 78.8 93.1 99.5 99.4 117.6 114.2 89.8 89.5 70.2 117.5 4727 2375 416 4227 4227 2555 383 249 405 144 119 2131 2 12 0 1457 1820 262 2197 Noise 429 347 291 327 362 0 392 453 226 346 35 15 236 232 47 182 303 56 216 86 142 74 10 0 97 193 737 129 151 217 351 217 54 Height 2065 951 5158 3602 3441 719 1935 64655 69234 143698 53 862328 719924 23155 912986 364964 362217 28219 24826 39272 27840 87551 275500 321033 316044 638573 56781 118435 IS Height 87551 275500 275500 275500 912986 912986 321033 316044 316044 638573 39272 27840 39272 27840 39272 28219 24826 56781 362217 362217 Sample size 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 362217 362217 362217 362217 362217 362217 362217 362217 362217 362217 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 2 .110 0 Factor2 Detection... 1 . 0 MMI 1 . 0 MM 1 . 0 MMI 1 . 0 bb 1 . 0 MM 1 . 0 MM 1 . 0 bbl 1 . 0 bb 1 . 0 MM 1 . 0 MM 1.0 1 . 0 MMI 1.0 1.0 1.0 1 . 0 bb 1 . 0 bb 1 . 0 MM 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb 1 . 0 bb Page 94 of 234 www.axysanalytical.corn Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-3,, Name: FC3G_013S023 Description: 1,WG42409,15/4000uL Date: 11Jan-2013 Time: 18:43:07 PFBA FC3G013S023 5.19 F4.12e2 o A 1897 %- 4.26 ! , ' v C /1 Vvv o- 'TrrrTi'ii'Tr,'.iIi:i,i:: fTljn 5.00 6.00 7.00 PFPeA FC3G 013S023 100n 6.03 6f 8 , 2.61e2 $ |\ i 1007 !". I p I %-i t 37 i n ; \i 0 - i!V ^.i.,,j !j, . ^ min 5.00 6.00 7.00 PFHxA FC3G 013S023 100-1 6.33 . 1.04e3 4276 %- 4.20 6.06 7.21 A- 0- 5.00 6.00 7.00 PFNA FC3G 013S023 7.55 7 .3 0 e 2 l 3171 OOI 1 %- 6.63 -C--- _ ^ o- i ___ i t min 7.00 8.00 PFDA FC3G 013S023 100- 7.99 F 8.81 e3 31602 %: I 0^ 8.00 ,, IiiiTr min 10.00 12.00 PFUnA FC3G_013S023 100q . 8.55 9.21e3 39709 %: - 0-1 8.00 10.00 12.00 PFHxS-1 FC3G 013S023 100- 7.33 2.36e2~i 951 % - 6.63 / 07.00 7.84 - !A' '.' -; .> rr mm. 8.00 PFHxS-2 FC3G 013S023 100- 7.38 1.93e2 638 6.63 / o - V 5-95; . m 7.00 i - min 8.00 PFHxS-3 FC3G_013S023 100q 7.29 %: o: 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13, Note: Calver = ng abs. SPM = ng/mL. oo _l_l PFHpA FC3G 013S023 6.68 1.42e3 4691 %- 4.82 6-P8j 0- min 5.00 6.00 7.00 PFOA-1 FC3G 013S023 io -] %: 1 o: 7.00 7.72 8.00 PFOA-2 FC3G 013S023 PFDoA FC3GJ313S023 100- r 9.37e3 : 41556 ; l _ ;i m min 8.00 10.00 12.00 PFBS-1 FC3G 013S023 100%- 6-26e2l 6.40 2526 1 6.26e2 2526 4.97 0-J^-^WWirnTn^Tr^-tTiTT min 5.00 6.00 7.00 PFBS-2 FC3G 013S023 ioo-q 4.11 6.40 '6.51 6.29 j %-? *5 .8 44 oi 5.00 6.00 7.00 PFOS-1 FC3G_013S023 fi oo 100 ' 1.28e5 :; 441869 -- --------------------- min 8.00 10.00 12.00 PFOS-2 FC3G 013S023 100- F 1.01 e5 jj 365644 j| j- V 0 - l-ffTiVp-rTTTTTTTT--TTTT'i'"= min 8.00 10.00 12.00 PFOSA FC3G013S023 100-j 9.99 2 .4 0 e 3 l : 11986 S %- o- U 8.00 10.00 12.00 m Page 95 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qid Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-3,, Name: FC3G_013S023 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:43:07 13C2-PFOA FC3G013S023 100-] 7n ^2.45e5 976119 13C8-PFOA FC3G 013S023 100n A Q67e4 387742 %: 13C4-PFOA FC3G013S023 100^1 - 7.09 | 1.04e5 1417419 %" -T-T min 0- -r-T min 0- 7.00 8.00 7.00 8.00 7.00 8.00 1802-PFHxS-2 FC3G013S023 100- _ 7.29 7.97o3 B 30547 %- 13C4-PFBA FC3G013S023 -1innn 25.'8129e4 104299 13C2-PFHxA FC3G 013S023 .. -----, 7.00 8.00 13C8-PFOSA FC3G_013S023 100n 9.96 1.10e4 57773 %- 5.00 6.00 7.00 13C2-PFOUEA FC3G_013S023 100-- 4 7.38 .02e 4 _ _| 152155 % 8.00 10.00 12.00 7.00 + 7 min 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G013S023 100- 8.29 I" 9.66e3 I 36307 %- 13C4-PFOS-2 FC3G 013S023 100- 8.29 8.53e3 31537 %- 1802-PFHxS-l FC3G_013S023 100n 7.33 1.21e4 ,\ -47202 ; %- 8.00 10.00 12.00 0- V . min 8.00 10.00 12.00 0J 7.00 8.00 13C5-PFNA FC3G013S023 7 4Q 100- 9:51 e4 Ik 387360 f \ r ::\ 4 min 7.00 8.00 13C2-PFDA FC3G 013S023 100- F 1.06e5 I 399588 % - 1m II 0 - ........ . I . 1......... min 8.00 10.00 12.00 13C2-PFDOA FC3G_013S023 100-n 9.15 1 1,76e5 - 8 790930 %: 0 -1 J L l. 8.00 10.00 12.00 m Page 96 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-3,, Name: FC3G_013S023 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 18:43:07 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-l 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.19 27 262.98 >218.... 6.03 27 312.97> 268.98 6.33 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.55 29 512.96 > 468.... 7.99 29 562.96 >518.... 8.55 30 612.95 >568.... 9.15 24 298.94> 79.96 6.40 25 298.94 > 98.95 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.38 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 37 1.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.29 21 216.99>171.98 5.19 21 314.97>269.98 6.33 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 412 261 1037 1423 1250 198 730 8808 9206 9371 626 236 193 128334 100794 2400 245065 96717 104112 9661 8533 12055 7974 28217 73559 95124 106243 175660 10995 40164 IS Area 28217 73559 73559 73559 245065 245065 95124 106243 106243 175660 12055 7974 12055 7974 12055 9661 8533 10995 104112 104112 104112 104112 104112 104112 104112 104112 104112 104112 104112 104112 ng/g 0.039 0.091 0.053 0.081 0.000 0.577 0.581 0.366 0.623 0.239 0.134 185.733 197.650 2.246 36.534 7.364 6.652 14.374 15.572 18.279 18.180 13.106 14.294 13.762 11.499 11.520 7.703 7.032 LOD 0.0164 0.2827 0.0938 0.0709 0.0078 0.0198 0.1737 0.0970 0.2486 0.0342 0.2233 0.0207 0.0490 0.0298 0.0220 0.0051 0.0060 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 9 4 16 16 17 2 24 216 188 188 50 7 0 102.5 124.0 112.0 80.7 87.4 102.6 102.0 110.3 120.3 115.8 96.8 97.0 64.8 142.1 2469 1599 305 1535 2825 1008 233 444 215 1522 162 2366 856 1087 1763 5984 3531 Noise 222 242 260 285 189 712 132 146 212 221 51 33 139 0 19 179 229 39 636 137 414 156 71 219 20 643 142 452 368 449 10 43 Height 1897 1007 4276 4691 3144 1078 3171 31602 39709 41556 2526 951 638 441869 365644 11986 976119 387742 417419 36307 31537 47202 30547 104299 336870 387360 399588 790930 57773 152155 IS Height 104299 336870 336870 336870 976119 976119 387360 399588 399588 790930 47202 30547 47202 30547 47202 36307 31537 57773 417419 417419 417419 417419 417419 417419 417419 417419 417419 417419 417419 417419 Sample size 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 bbl 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 bb 1.0 1.0 MM 1.0 MM 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 97 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\1nst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-4,, Name: FC3G_013S024 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:00:08 oo J < CO PFBA FC3G 013S024 6.16 f3 . . . TU 6.44 : ,6.55 PFPeA FC3G_013S024 100^1 ; %: 5.88 7.60O2 2198 6.68 4.82 | \ V, 11 ' 5.00 6.00 7.00 5.00 6.00 7.00 PFNA FC3G 013S024 PFDA FC3G_013S024 100n _ 7.99 7.44e3 26886 PFHxA FC3GJ313S024 6.33 PFUnA FC3G 013S024 7.00 8.00 PFHxS-1 FC3GJ)13S024 8.00 10.00 12.00 PFHxS-2 FC3G_013S024 PFHxS-3 FC3G_013S024 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S024 100- 6.68 9.53e2 f; 4513 ; % - 6.46 ' : 4 .0 5 7 --A-/,' 0 - ....... ............... -- ......... mtn 5.00 6.00 7.00 PFOA-1 FC3G 013S024 100n 7.09 9.1802 I 3399 %- / \ 7.78 07.00 r-r min 8.00 PFOA-2 FC3G_013S024 100- 7.49 3.50e2 A 1493 \ %z_ 7.05 I 07.00 8.00 PFDoA FC3G_013S024 100- y. io i"5.66e3 24297 ^ min 8.00 10.00 12.00 PFBS-1 FC3G 013S024 100^ 1 .2 2 e 2 l 389 1 %4.74 5.15 \-- 0 - -mr mtn 5.00 6.00 7.00 PFBS-2 FC3G 013S024 iooq c 5.62 5.11 / 6.25 % - 7.07 o4- w*_ i _ r 5.00 6.00 7.00 PFOS-1 FC3G 013S024 100- 8.29 r 7.20e3 26066 ; %" PFOS-2 FC3G_013S024 100-q - 8.29 f 5.77e3 I 21333 %: PFOSA FC3G_013S024 100- 9.96 __ 5.63e2 3178 / 8.00 10.00 12.00 T^fTr min 8.00 10.00 12.00 8.00 10.00 12.00 m Page 98 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-4,, Name: FC3GJJ13S024 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:00:08 13C2-PFOA FC3G013S024 100-1 7.09 *~2.00e5 765690 %: 13C8-PFOA FC3G 013S024 100- 7.09 A 7.54e4 295525 13C4-PFOA FC3G 013S024 100- 7.09 8.52e4 314798 %- 07.00 -- min 8.00 0 - . TTTT . min 7.00 8.00 o7.00 ! 8.00 1802-PFHxS-2 FC3G 013S024 13C4-PFBA FC3G__013S024 100- 5.24 f 1.20e4 I 48378 %- 4.44 OJ-n-rrTM 5.00 6.00 7.00 13C2-PFHxA FC3G 013S024 100- 6.34 5.91 e 4 l 279176 (i %- o: - - 5.00 6.00 7.00 13C8-PFOSA FC3G_013S024 inn 996 1001 6.04e3~| 36778 13C2-PFOUEA FC3G Q13S024 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S024 100- 8.29 9.36e3 35614 %- 8.00 10.00 12.00 13C4-PFOS-2 FC3G 013S024 100" 8.29 7.32e3 ; 27933 %-: 1 :\ O-J V... 8.00 10.00 min 12.00 1802-PFHxS-l FC3G 013S024 100n 7.29 A 9.44e3 ; 37181 %- 0J / 7.00 V .. 8.00 13C5-PFNA FC3G_013S024 7 fie . 100 7.43e4 "A 293409 l?l min 7.00 8.00 13C2-PFDA FC3G 013S024 100- I 8.67e4 I 325629 %- H 0 ip& j min 8.00 10.00 12.00 13C2-PFDoA FC3G_013S024 100-, 9.15 f 1.19e5 I 517853 %- 0-" 8.00 10.00 12.00 m Page 99 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\inst_G\PFC.PRO\FC\FC3G..013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-4,, Name: FC3G_013S024 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:00:08 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 >218.... 5.88 27 312.97> 268.98 6.33 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 >518.... 8.55 30 612.95 > 568.... 9.15 24 298.94> 79.96 6.49 25 298.94 > 98.95 24 398.84 > 79.96 7.33 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 37 1.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.24 21 314.97>269.98 6.34 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 393 760 1087 953 918 332 7437 8414 5661 122 111 7204 5765 563 199891 75395 85160 9356 7322 9439 6463 11980 59067 74288 86699 119050 6037 31284 IS Area 11980 59067 59067 59067 199891 199891 74288 86699 86699 119050 9439 6463 9439 6463 9439 9356 7322 6037 85160 85160 85160 85160 85160 85160 85160 85160 85160 85160 85160 85160 ng/g 0.188 0.062 0.036 0.590 0.648 0.281 0.112 0.130 10.208 12.433 0.846 35.551 6.848 5.310 16.608 15.941 17.074 17.579 6.638 13.693 12.822 11.195 9.314 5.046 5.345 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0457 0.2420 0.0417 0.0811 0.0084 0.0472 0.1262 0.0676 0.1923 0.5266 0.0373 0.0447 0.0349 0.0322 0.0195 0.0202 %Rec S/N 4 6 14 14 4 5 107 122 100 16 2 102.2 118.1 91.6 95.5 91.7 98.2 101.1 57.3 118.1 110.6 96.6 80.3 43.5 110.6 176 271 145 2650 269 1891 1086 391 808 309 41 1407 920 977 1023 1279 792 Noise 320 373 308 319 801 242 281 252 315 242 25 33 251 34 79 148 79 22 289 1100 166 33 71 46 87 1171 198 319 333 506 29 147 Height 1408 2198 4252 4513 3399 1547 26886 38339 24297 389 444 26066 21333 3178 765690 295525 314798 35614 27933 37181 26914 48378 279176 293409 325629 517853 36778 116801 IS Height Sample size 48378 2.0700 279176 2.0700 279176 2.0700 279176 2.0700 765690 2.0700 765690 2.0700 293409 2.0700 325629 2.0700 325629 2.0700 517853 2.0700 37181 2.0700 26914 2.0700 37181 2.0700 26914 2.0700 37181 2.0700 35614 2.0700 27933 2.0700 36778 2.0700 314798 2.0700 314798 2.0700 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 314798 2.0700 2.0700 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 MMI 1.0 MM 1.0 MM 1.0 MM-I 1.0 MMI 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 1.0 MM 1.0 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 100 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-5,, Name: FC3G_013S025 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:17:09 PFBA FC3G_013S025 100- 6.27 j\ " j \ 6.47 % - 4.11 c V o-1 5.00 6.00 7.00 PFPeA FC3G 013S025 6.08 6.87 PFHxA FC3G 013S025 100^ 6.34 1 .1 0 e 3 l 5487 J : %-1 4.054.78 7.21 5.00 6.00 7.00 5.00 6.00 7.00 PFNA FC3G 013S025 100- 7.49 1,49e3 5940 %S.63 / -.j 1111 1111 7.00 \ 8.20 8.0 PFDA FC3G 013S025 100- 8.05 f 1.43e4 i 48641 % ' l! PFUnA FC3G 013S025 100- 8.58 f 1.54e4 li 66895 0 - i I i i i <|r i iT| n r / j rm j >, ll|||| 8.00 10.00 12.00 8.00 10.00 PFHxS-1 FC3G_013S025 PFHxS-2 FC3G_013S025 100- 7.33 7.78e1 370 %- PFHxS-3 FC3G_013S025 o7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3GJ313S025 100- 6.73 9.67e2 ! 3936 Ji'; %- ^035.43 6.38 ; 0- 5.00 6.00 7.00 PFDoA FC3G_013S025 9.18 100^ fl.2 1 e 4 I 47959 8.00 10.00 12.00 PFOS-1 FC3G_013S025 100- 8.35 4.30e4 ; 149463 %- 8.00 10.00 12.00 PFOA-1 FC3G_013S025 100 7.09 jV 5.96e2 ; . 2611 6.63 . % 7.90 7.00 8.00 PFBS-1 FC3G_013S025 6.38 100- 638 9.18e1 9-18e1 I 654 4.05 654 5.49 y i 6.63 5.00 6.00 7.00 PFOS-2 FC3G_013S025 100 8.35 r 3.31 e4 * 119154 8.00 10.00 12.00 PFOA-2 FC3G 013S025 PFBS-2 FC3G_013S025 100 6.53 4.56e1 ~~| 328 6.53 0/o/. 4.56e1 5.15 .5.43 328 /\ 5.00 6.00 7.00 PFOSA FC3G_013S025 100- 10.02 _ 3.21e2 1438 %- 8.00 10.47 12.32 min 10.00 12.00 m Page 101 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-5,, Name: FC3G_013S025 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:17:09 13C2-PFOA FC3G_013S025 100- 7 n 2.34e5 . 951217 %- " l lills 7.00 -T-T min 8.00 13C8-PFOA FC3G 013S025 100- f 8.51e4 339700 l% - 0 - + r , -- ^ r - - n min 7.00 8.00 13C4-PFOA FC3G_013S025 100q - 7.09 , 8.94e4 1356871 %- 0-7.00 8.00 1802-PFHxS-2 FC3G 013S025 m10n0l 7 .74-353e '-r 1292751 %1 J iji, o1. ,, 7.00 - min 8.00 13C4-PFBA FC3G 013S025 100- 5.21 F 2.70e4 97791 %- 4.09 0- --W 5.00 6.00 m min 7.00 13C2-PFHxA FC3G_013S025 100 6.36 7.07e4 336367 i % 0 5.00 6.00 7.00 13C8-PFOSA FC3G 013S025 100- 9.96 1.07e4~| 58121 | I%J 13C2-PFOUEA FC3G_013S025 100n 7.38 3.57e4 | 132790) I %: l > . min 8.00 10.00 12.00 07.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S025 100^ 8.35 f 1.01e4 \ 37878 %- ; ,\ 0J 8.00 10.00 min 12.00 13C4-PFOS-2 FC3G_013S025 100n : 8.29 I 8.43e3 32036 %- 0 min 8.00 10.00 12.00 1802-PFHxS-l FC3G_013S025 13C5-PFNA FC3G_013S025 7 AO 100 1 8.24e4 ~k 334947 j %- ----------- --/-------',' . . mjn 7.00 8.00 13C2-PFDA FC3G 013S025 100- 8.81e4 I 321510 %- A0J ......i ................ min 8.00 10.00 12.00 13C2-PFDOA FC3G013S025 100 J : 9.18 I 1.57e5 i: 692100 % " 0- '.-- 8.00 10.00 12.00 m Page 102 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G__013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-5,, Name: FC3G_013S025 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:17:09 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 RFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.21 27 262.98 > 218.... 5.90 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.05 28 462.96 > 418.... 7.49 29 512.96 > 468.... 8.05 29 562.96 >518.... 8.58 30 612.95 >568.... 9.18 24 298.94> 79.96 6.38 25 298.94 > 98.95 6.53 24 398.84 > 79.96 25 398.84> 98.96 7.33 24 398.84> 118.70 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 10.02 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371,9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.21 21 314.97>269.98 6.36 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.18 21 506>78 9.96 458.98 >393.98 7.38 Area 479 130 1102 967 596 80 1493 14267 15437 12077 92 46 78 43024 33135 321 233942 85143 89444 10137 8431 9817 7447 26981 70705 82434 88125 156813 10660 35672 IS Area 26981 70705 70705 70705 233942 233942 82434 88125 88125 156813 9817 7447 9817 7447 9817 10137 8431 10660 89444 89444 89444 89444 89444 89444 89444 89444 89444 89444 89444 89444 ng/g 0.011 0.040 0.012 0.103 1.278 1.226 0.649 0.065 56.073 62.068 0.162 38.499 7.156 5.420 16.648 16.985 16.432 18.741 13.833 15.166 13.165 10.529 11.352 8.244 5.923 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0129 0.0578 0.0126 0.0166 0.0241 0.1081 0.0770 0.1141 0.3516 0.0344 0.0487 0.0127 0.0256 0.0529 0.0380 %Rec S/N 6 4 15 25 6 2 11 194 229 209 17 25 10 113.9 127.0 96.2 98.5 100.5 97.2 110.9 122.8 134.6 116.8 93.4 100.8 73.2 126.2 1096 1001 77 9314 1293 674 433 631 431 0 107 1576 826 2267 1474 639 467 Noise 271 223 359 157 402 213 529 251 292 229 39 13 76 37 171 136 119 19 102 263 530 88 51 91 0 912 213 405 142 469 91 284 Height 1747 999 5487 3936 2611 409 5940 48641 66895 47959 654 328 370 149463 119154 1438 951217 339700 356871 37878 32036 39075 29275 97791 336367 334947 321510 692100 58121 132790 IS Height 97791 336367 336367 336367 951217 951217 334947 321510 321510 692100 39075 29275 39075 29275 39075 37878 32036 58121 356871 356871 356871 356871 356871 356871 356871 356871 356871 356871 356871 356871 Sample size 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 2.1300 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 bbl 1.0 bb 1.0 MMI 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMI 1.0 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 103 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-6,, Name: FC3G_013S026 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:34:02 PFBA FC3G 013S026 PFPeA FC3G_013S026 100-1 r 4 '26 5.92.6.06 k. ,,6.97 5.65. .i a, | / PFHxA FC3G 013S026 PFNA FC3G 013S026 5.00 6.00 7.00 PFDA FC3G 013S026 PFUnA FC3G 013S026 PFHxS-1 FC3G 013S026 PFHxS-2 FC3G 013S026 PFHxS-3 FC3G_013S026 100 7.84 7.46 A : b,63 %- 7.29. 0 \ , , /, 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S026 100- 6.73 8.60e2 .. 3419 r; %4.52 5.92 ; X 0 - !................. ............mminin 5.00 6.00 7.00 PFOA-1 FC3G 013S026 100- 7.05 \ 8.32e2 \ 3366 %- 8.08 u 0--L_._..._....... ................-.min min 7.00 8.00 PFOA-2 FC3G 013S026 100- 7.09 2.01 e2 \ 784 %- " , / \ 7.61 U -o--------------- --....'..... - - - 7.00 8.00 PFDoA FC3G_013S026 PFBS-1 FC3G_013S026 100- 6.46 6.19o2 2777 . %- ' ; 0 - -- :-- Jj---V1 - min 5.00 6.00 7.00 PFBS-2 FC3GJM3S026 100- 6.46 3.25e2~| 1815 1 %- 4.03 / 0 - Wrrmftiw 5.00 i min 6.00 7.00 PFOS-1 FC3G_013S026 QOO 100n] r 4.32e4 1 151255 J [fei jfi \ - - U r ^ ........ min 8.00 10.00 12.00 PFOS-2 FC3G 013S026 100- f 3.62e4 j 137664 I 11 0J 8.00 10.00 min 12.00 PFOSA FC3G_013S026 100- 2 .6 8 e 3 l 14943 %: 0J " 8.00 10.00 12.00 m Page 104 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-6,, Name: FC3G_013S026 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:34:02 13C2-PFOA FC3G013S026 100n 7.09 , 1.95e5 I 788725 %- 13C8-PFOA FC3G013S026 100- 7.09 7.85e4 1 322576 %- 13C4-PFOA FC3G_013S026 100- 7.09 |f~8.31e4 i 333309 %- 0 7.00 8.00 if U 7.00 8.00 7.00 8.00 1802-PFHxS-2 FC3G013S026 100-i, 7 fs6r .4 6 e93 26787 - . I, . . |'f-,'rrr."",'::7-| i , , min 7.00 8.00 13C4-PFBA FC3G 013S026 1u0 0 -- 1.89e4 68865 %- 4.07 0 ------ tf~M7TTmTTnT min 5.00 6.00 7.00 13C2-PFHxA FC3G013S026 100 ] : 6.33 5.42e4 I 256975 1 %: 0-* 'T W " 5.00 6.00 7.00 13C8-PFOSA FC3G 013S026 100- 9.99 6 .7 3 e 3 l 41583 I %- | 13C2-PFOUEA FC3G 013S026 100- 7.38 2.52e4 101418 %" - 8'.0r 0; 1!' `m 1 10.00 l 11! 111 12.00 mln - : 'I 7.00 kfes. i f 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S026 100- 8.29 9.52e3 35987 %- 13C4-PFOS-2 FC3G_013S026 100- 8.29 i 6.84e3 I 26777 1802-PFHxS-1 FC3G013S026 100'- 7.33 9.10e3 36017 %- Au 8.00 10.00 12.00 o-Mm 8.00 10.00 12.00 7.00 8.00 13C5-PFNA FC3G_013S026 7 ACt 100 6.77e4"l 278050j \ min 7.00 8.00 13C2-PFDA FC3G 013S026 100- I 8.10e4 i; 304410 i'i 0 - ^ --------------------- min 8.00 10.00 12.00 13C2-PFDoA FC3G_013S026 100q - 9.18 1.23e5 540335 %: 0-* 8.00 10.00 12.00 m Page 105 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-6,, Name: FC3G_013S026 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:34:02 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 27 262.98 > 218.... 27 312.97> 268.98 6.33 27 362.97> 318.98 6.73 19 412.97> 368.98 7.05 19 412.97> 168.80 7.09 28 462.96 >418.... 7.46 29 512.96 >468.... 7.99 29 562.96 >518..,. 8.58 30 612.95 >568.... 9.18 24 298.94> 79.96 6.46 25 298.94 > 98.95 6.46 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.99 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.29 21 216.99>171.98 5.26 21 314.97>269.98 6.33 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.18 21 506>78 9.99 458.98 >393.98 7.38 Area 934 860 832 201 1768 10861 11689 8822 619 325 165 58 43210 36210 2680 195145 78535 83131 9518 6844 9096 6455 18860 54250 67679 81022 123142 6725 25160 IS Area 18860 54250 54250 54250 195145 195145 67679 81022 81022 123142 9096 6455 9096 6455 9096 9518 6844 6725 83131 83131 83131 83131 83131 83131 83131 83131 83131 83131 83131 83131 ng/g 0.056 0.023 0.103 0.175 0.984 0.955 0.559 0.763 0.338 0.201 57.825 80.687 3.870 33.302 6.845 4.855 16.211 14.297 15.787 16.846 10.027 12.067 11.209 10.038 9.244 5.393 4.027 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.2621 0.1474 0.0243 0.0059 0.0437 0.0316 0.1391 0.0719 0.1590 0.3346 0.0132 0.0064 0.0246 0.0376 0.0201 0.0056 %Rec S/N 13 23 49 0 17 108 126 143 93 150 2 36 102.2 126.1 89.4 99.5 87.8 96.9 103.4 92.3 111.1 103.2 92.4 85.1 49.7 89.0 922 412 1455 4138 3572 333 1450 301 650 329 82 3243 5354 1147 807 1240 2154 Noise 272 276 343 147 69 0 416 361 411 279 30 12 278 8 89 164 334 10 191 90 1000 25 89 55 81 841 79 52 265 670 34 47 Height 4550 3419 3366 784 6997 39111 51701 39773 2777 1815 632 293 151255 137664 14943 788725 322576 333309 35987 26777 36017 26787 68865 256975 278050 304410 540335 41583 101418 IS Height Sample size 68865 2.2100 256975 2.2100 256975 2.2100 256975 2.2100 788725 2.2100 788725 2.2100 278050 2.2100 304410 2.2100 304410 2.2100 540335 2.2100 36017 2.2100 26787 2.2100 36017 2.2100 26787 2.2100 36017 2.2100 35987 2.2100 26777 2.2100 41583 2.2100 333309 2.2100 333309 2.2100 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 333309 2.2100 2.2100 Factor2 Detection, 1.0 1.0 1.0 MMI 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 106 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-7,, Name: FC3G_013S027 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:51:03 PFBA FC3G_013S027 100 1 6.18 %-14.07 :V, |\ 6.83 \j . ................... min 5.00 6.00 7.00 PFPeA FC3G 013S027 100^ 2.78e2 k 1272 jl 6.73 % - V4-41 ( ^ / #w . 0 - .......... . min 5.00 6.00 7.00 PFHxA FC3G_013S027 100-j 6.40 _ 7.46e2~l 3332 I %-14'15 6-1 l 5.06 0J Vv 5.00 6.00 7.00 PFNA FC3G 013S027 PFDA FC3G_013S027 100- 7.99 f 7.43e3 I 26212 %4 -a 1 8.00 10.00 12.00 PFUnA FC3G_013S027 100- 8.55 6.99e3 30880 h 8.00 10.00 12.00 PFHxS-1 FC3G 013S027 PFHxS-2 FC3G__013S027 100 - 6-63 7.29 %- l 7.92e0 \ 55 8.08 PFHxS-3 FC3G 013S027 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G013S027fi 100- 6.43e2 3117 5.525.64 n- min 5.00 6.00 7.00 PFOA-1 FC3G 013S027 100-] %z \ 7.90 o' i ..................... .-V min 7.00 8.00 PFOA-2 FC3G_013S027 100q 7.14 ; 2.19e2 783 %1 8.02 07.00 8.00 PFDoA FC3G 013S027 PFBS-1 FC3G_013S027 100" ' %- 6.53 1 .9 9 e ll 6-53 1 5 1 |1.99e1 151 4.89 r 6.92 , ,, A a. M 5.00 6.00 7.00 PFBS-2 FC3G_013S027 100^4.39 5-25 6.38 7.02 %- 5.52 M/ ^i WV \ 5.00 6.00 7.00 PFOS-1 FC3G 013S027 100- 8.29 F 9.12e3 1S -32017 %\ H 1 PFOS-2 FC3GJ113S027 100-] 8.29 7.24e3 ' 26000 %_ PFOSA FC3G_013S027 100- 9.96 _ 3.10e2 I 1942 I %- 0^ min 8.00 10.00 12.00 0-- 4 ^ 8.00 10.00 12.00 0-+T 8.00 10.00 12.00 m Page 107 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-7,, Name: FC3G_013S027 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:51:03 13C2-PFOA FC3G013S027 100 I.89e5 728266 . i i ioo 13C8-PFOA FC3G 013S027 Ih 7.60c4 . 98632 %: 13C4-PFOA FC3G_013S027 100-, 7.09 I" 8.12e4 1317673 %- tt min 0: -T-T min 0- 7.00 8.00 7.00 8.00 7.00 8.00 1802-PFHxS-2 FC3G_013S027 100- 7.29 I"6.19e3 24515 %- 13C4-PFBA FC3G_013S027 5.21 100n r 2.23e4 84849 13C2-PFHxA FC3G 013S027 7.00 8.00 13C8-PFOSA FC3G_013S027 100- 9.96 5.75e3l 33818 %- I 5.00 6.00 7.00 13C2-PFOUEA FC3G 013S027 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S027 100- 8.29 l 8.77e3 . 34158 : 13C4-PFOS-2 FC3G_013S027 100- 8.29 f 6.62e3 j; 24410 %- 1802-PFHxS-l FC3G_013S027 100- 7.33 8.73e3 35598 %- 0- ' min 8.00 10.00 12:00 0 - min 8.00 10.00 12.00 0j 7.00 min 8.00 13C5-PFNA FC3G 013S027 100- 7.49 6 .8 9 e 4 l, 271280 H %- i i i l 0^ 7.00 min 8.00 13C2-PFDA FC3G 013S027 100- 7.99 I 7.86e4 1 279350 %- 0J min 8.00 10.00 12.00 1302-PFDoA FC3G 013S027 100- 9.15 8.10e4 356459 %: 1 jt! 0J i d . . 8.00 10.00 min 12.00 m Page 108 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-7,, Name: FC3G_013S027 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 19:51:03 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 1302-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.40 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.14 28 462.96 >418.... 7.49 29 512.96 > 468.... 7.99 29 562.96 > 518.... 8.55 30 612.95 > 568.... 9.18 24 298.94> 79.96 6.53 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84> 98.96 7.29 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.29 21 216.99>171.98 5.21 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 655 278 746 643 583 219 701 7427 6993 4453 20 8 9123 7242 310 189082 76015 81235 8774 6619 8732 6187 22264 54666 68870 78561 81044 5751 28458 IS Area 22264 54666 54666 54666 189082 189082 68870 78561 78561 81044 8732 6187 8732 6187 8732 8774 6619 5751 81235 81235 81235 81235 81235 81235 81235 81235 81235 81235 81235 81235 ng/g 0.066 0.026 0.134 0.027 0.701 0.609 0.393 14.455 18.156 0.440 36.671 7.529 5.269 16.984 15.714 17.225 18.351 13.452 13.819 12.962 11.062 6.914 5.242 5.058 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0329 0.0276 0.0185 0.0190 0.0107 0.0696 0.1506 0.1003 0.1541 0.5006 0.0276 0.0229 0.0309 0.0484 0.0135 0.0154 %Rec S/N 9 5 10 15 4 7 7 107 130 116 8 3 101.4 124.9 87.4 93.9 86.9 95.2 101.4 111.5 114.6 107.5 91.7 57.3 43.5 100.7 332 247 124 5872 1194 1482 729 295 537 362 79 1732 1713 978 482 1751 988 Noise 238 257 350 204 311 107 380 245 237 179 20 6 375 18 70 97 105 16 124 250 214 47 83 66 68 1080 142 158 286 740 19 106 Height 2240 1272 3332 3117 1242 783 2648 26212 30880 20764 151 55 32017 26000 1942 728266 298632 317673 34158 24410 35598 24515 84849 246658 271280 279350 356459 33818 104737 IS Height Sample size 84849 1.9900 246658 1.9900 246658 1.9900 246658 1.9900 728266 1.9900 728266 1.9900 271280 1.9900 279350 1.9900 279350 1.9900 356459 1.9900 35598 1.9900 24515 1.9900 35598 1.9900 24515 1.9900 35598 1.9900 34158 1.9900 24410 1.9900 33818 1.9900 317673 1.9900 317673 1.9900 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 317673 1.9900 1.9900 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 MMI 1.0 bb 1.0 MMI 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MMI 1.0 1.0 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 109 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-8,, Name: FC3GJJ13S028 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:07:56 PFBA FC3G_013S028 100- 6.25 6.36 4.05 j p 'A j \ PFPeA FC3G 013S028 o5.00 6.00 7.00 PFNA FC3G 013S028 PFDA FC3G 013S028 PFHxS-1 FC3G 013S028 PFHxS-2 FC3G 013S028 PFHxA FC3G 013S028 PFUnA FC3G..013S028 1nn 8.55 100 f 5.85e3 J 27357 Ml 8.00 10.00 12.00 PFHxS-3 FC3G 013S028 PV by LHE, SV by KJC 31-Jan-13. Note: Caiver = ng abs. SPM = ng/mL. PFHpA FC3G 013S028 PFOA-1 FC3G 013S028 PFOA-2 FC3G 013S028 PFDoA FC3G_013S028 100-, _ 9.12 j 4.44e3 I 17756 %- !; 8.58 ?, --8.00 10.00 12.00 PFBS-1 FC3G_013S028 100-, 6.47 4.59el' 500 %4.57 6.73 6.23 \1 '\ 5.00 6.00 7.00 PFBS-2 FC3G_013S028 100- 6.46 4.06e1 392 %~ ;4.44 6-87 6.06 \ A 5.00 6.00 7.00 PFOS-1 FC3G_013S028 OQ 100-], F 9;01e3 1 30840 %- 1 j min 8.00 10.00 12.00 PFOS-2 FC3G_013S028 100n F 6.88e3 J 26051 %: I 0-> '<i -T min 8.00 10.00 12.00 PFOSA FC3G 013S028 100- 1.18e3l 6237 J %L i 08.00 10.00 12.00 m Page 110 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\l nst G\PFC.PRO\FC\FC3G_013-B .qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-8,, Name: FC3G_013S028 Description: 1,WG42409,15/4000uL Date: 11>Jan-2013 Time: 20:07:56 oo 13C2-PFOA FC3G 013S028 7.09 2.10e5 859509 %L 0 - t4-A------" -- r r i . min 7.00 8.00 cop 13C8-PFOA FC3G 013S028 7.09 " 7.96e4 317815 %: 13C4-PFOA FC3G 013S028 100q 7.09 8.72e4 353756 %: 07.00 | T-r min 8.00 10 - -. .J i 7.00 , 8.00 1802-PFHxS-2 FC3G_013S028 100'--|67.6.373e3_l| ` 26962 l | 13C4-PFBA FC3G013S028 100- _ 5.21 I" 1.73e4 I 67531 13C2-PFHxA FC3G 013S028 7.00 8.00 13C8-PFOSA FC3G_013S028 100- 9.93 6.55e3 j 38092 %- 4.09 ' 1V 111r T r 7TTtT'T 5.00 6.00 7.00 13C2-PFOUEA FC3G 013S028 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM - ng/mL. 13C4-PFOS-1 FC3G_013S028 100- a OQ 9.56e3 ;; 36848 \\ 8.00 min 10.00 12.00 13C4-PFOS-2 FC3G 013S028 100n f 8.05e3 j'j 31110 %: 0 min 8.00 10.00 12.00 1802-PFHxS-l FC3G 013S028 100- 1.00e4 A 41294 ; %- 07.00 8.00 13C5-PFNA FC3G 013S028 13C2-PFDA FC3G_013S028 100- 7.99 9.40e4 337660 %- ; 13C2-PFDoA FC3G__013S028 inn 9-15 100 1.11e5 I 477070 %- ;\ 8.00 10.00 12.00 8.00 10.00 12.00 m Page 111 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-8,, Name: FC3G_013S028 Description: 1,WG42409,15/4000uL Date: 11>Jan-2013 Time: 20:07:56 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.15 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 >468.... 8.05 29 562.96 >518.... 8.55 30 612.95 > 568.... 9.12 24 298.94> 79.96 6.47 25 298.94 > 98.95 6.46 24 398.84 > 79.96 25 398.84> 98.96 7.29 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.93 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.21 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.93 458.98 >393.98 7.38 Area 439 290 965 958 721 238 480 4560 5852 4440 46 41 26 9005 6878 1179 209723 79588 87243 9559 8048 9999 6671 17346 57236 76367 93971 111210 6546 31775 IS Area 17346 57236 57236 57236 209723 209723 76367 93971 93971 111210 9999 6671 9999 6671 9999 9559 8048 6546 87243 87243 87243 87243 87243 87243 87243 87243 87243 87243 87243 87243 ng/g 0.061 0.065 0.123 0.230 0.366 0.176 0.006 12.265 13.213 1.740 35.551 6.890 5.311 16.172 16.701 17.238 17.293 9.161 12.646 12.563 11.565 8.293 5.215 5.301 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0295 0.0261 0.0474 0.0238 0.0174 0.0635 0.1405 0.0993 0.4506 0.2291 0.0380 0.0259 0.0138 0.0266 0.0269 0.0076 %Rec S/N 4 5 13 23 6 0 7 91 181 100 6 17 3 104.7 121.7 93.8 95.2 98.3 101.5 101.8 80.9 111.7 111.0 102.2 73.3 46.1 112.4 257 461 496 2273 856 914 726 340 531 115 115 1129 1402 2228 988 835 2097 Noise 556 266 301 197 399 0 264 167 151 177 86 23 221 30 0 120 56 13 378 371 387 51 92 78 235 586 232 212 152 483 46 57 Height 2139 1198 3781 4554 2273 1295 1791 15137 27357 17756 500 392 91 30840 26051 6237 859509 317815 353756 36848 31110 41294 26962 67531 262250 297757 337660 477070 38092 120193 IS Height 67531 262250 262250 262250 859509 859509 297757 337660 337660 477070 41294 26962 41294 26962 41294 36848 31110 38092 353756 353756 353756 353756 353756 353756 353756 353756 353756 353756 353756 353756 Sample size 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 2.1200 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 bbl 1.0 bb 1.0 bbl 1.0 MM 1.0 MMI 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MMI 1.0 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 112 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-9,, Name: FC3G_013S029 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:24:57 PFBA FC3G 013S029 100n 5 6.31 %- 4.15 | A ,,6.51 |V \7 .1 6 ;V\vyL J 0- 5.00 6.00 7.00 PFPeA FC3G013S029 ioon 6.03 3.29e2l 1 1726 I 6.57 %- 4.70 - 'J qJ M * 5.00 6.00 7.00 PFNA FC3G 013S029 PFDA FC3G013S029 100- 8.05 9.52e3 33264 %- PFHxS-1 FC3G 013S029 8.00 10.00 12.00 PFHxS-2 FC3G 013S029 PFHxA FC3G_013S029 100- 6.38 _ 1.06e3 5748 I"! " 4 15 :/ 5.69 \ 7.21 5.00 6.00 7.00 PFUnA FC3G013S029 100- _ 8.58 F 1.09e4 I 44403 %- JL 8.00 10.00 12.00 PFHxS-3 FC3G 013S029 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S029 PFDoA FC3G 013S029 PFOA-1 FC3G_013S029 100- 7.14 9 . 8e2 a 3080 %- V p 7.96 7.00 8.00 PFBS-1 FC3G_013S029 100- 6.46 1.54e3 ?. 6261 : % PFOA-2 FC3G 013S029 PFBS-2 FC3G_013S029 100- 6.44 _ 7.29e2 3294 %- ; 5.00 6.00 7.00 5.00 6.00 7.00 PFOS-1 FC3G_013S029 o 'IC. 100 "" 1.9265 5 588070 / V min 8.00 10.00 12.00 PFOS-2 FC3G 013S029 100 i 1.42e5 476013 % \\ ! 0 8.00 ------------------- min 10.00 12.00 PFOSA FC3G_013S029 100q 9.99 4.43e4l - 243228 I %: 0-" i " " i ' r 8.00 10.00 12.00 m Page 113 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-9,, Name: FC3G_013S029 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:24:57 13C2-PFOA FC3G_013S029 100 I.81e5 '716397 - 13C8-PFOA FC3G 013S029 100- i 7.07e4 277888 %- op 13C4-PFOA FC3G013S029 7.09 i 7.70e4 1306024 %- tt min 0 - ----- ~7~V-l.I'-n min qJ 7.00 8.00 7.00 8.00 7.00 8.00 1802-PFHxS-2 FC3G 013S029 13C4-PFBA FC3G_013S029 100- 5.21 2.40e4 94855 % -4.07 13C2-PFHxA FC3G 013S029 13C8-PFOSA FC3G_013S029 100n 7993e3l 45487 j % ~l if 5.00 6.00 7.00 13C2-PFOUEA FC3G 013S029 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S029 100 8.35 7.01 e3 : 26814 % ; rrrrr min 8.00 10.00 12.00 13C4-PFOS-2 FC3G_013S029 100q - 8.35 6.71 e3 25102 %- o: M 8.00 10.00 12.00 13C5-PFNA FC3G_013S029 13C2-PFDA FC3G013S029 100 7.99 7.52e4 265288 1802-PFHXS-1 FC3G_013S029 100- 7.33 -8.65e3 35036 ; \ %- 7.00 8.00 13C2-PFDoA FC3G_013S029 100 9.18 1.16e5 524026 % ttht min 8.00 10.00 12.00 0- 8.00 10.00 12.00 m Page 114 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday. January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-9,, Name: FC3G_013S029 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:24:57 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 1302-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.13 27 262.98 > 218. .. 6.03 27 312.97> 268.98 6.38 27 362.97> 318.98 6.68 19 412.97> 368.98 7.14 19 412.97> 168.80 7.09 28 462.96 >418.... 7.46 29 512.96 >468.... 8.05 29 562.96 > 518.... 8.58 30 612.95 >568.... 9.18 24 298.94> 79.96 6.46 25 298.94 > 98.95 6.44 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.29 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.21 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 >569.... 9.18 21 506>78 9.99 458.98 >393.98 7.38 Area 580 329 1061 1056 908 136 377 9522 10853 8757 1536 729 575 305 189 191824 141946 44276 180728 70744 77041 7010 6708 8654 5844 24010 60045 56653 75219 115837 7925 25845 IS Area 24010 60045 60045 60045 180728 180728 56653 75219 75219 115837 8654 5844 8654 5844 8654 7010 6708 7925 77041 77041 77041 77041 77041 77041 77041 77041 77041 77041 77041 77041 ng/g 0.064 0.068 0.046 0.065 0.905 0.942 0.600 2.040 1.464 0.783 0.554 0.550 345.330 319.648 55.423 32.834 6.564 4.439 12.711 14.918 15.992 16.237 13.590 14.219 9.989 9.921 9.258 6.766 4.081 LOD 0.0075 0.0739 0.0706 0.1068 0.0084 0.0243 0.0095 0.0744 0.0731 0.0882 0.0119 0.2418 0.0209 0.0438 0.0310 0.0437 0.0170 0.0132 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec 102.2 122.5 82.9 79.1 92.8 99.5 101.0 126.8 132.7 93.2 92.6 86.4 63.2 91.4 S/N 8 12 21 18 16 3 3 117 197 192 53 246 18 14 9 5655 2008 1753 4051 2044 179 502 478 4112 0 168 2280 616 851 723 1730 927 Noise 278 148 278 201 189 195 437 285 226 201 118 13 124 110 72 104 237 139 177 136 1709 53 53 9 0 566 117 329 312 724 26 113 Height 2156 1726 5748 3713 3080 545 1391 33264 44403 38464 6261 3294 2186 1583 633 588070 476013 243228 716397 277888 306024 26814 25102 35036 22950 94855 266205 202602 265288 524026 45487 104582 IS Height 94855 266205 266205 266205 716397 716397 202602 265288 265288 524026 35036 22950 35036 22950 35036 26814 25102 45487 306024 306024 306024 306024 306024 306024 306024 306024 306024 306024 306024 306024 Sample size 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 2.2400 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 bbl 1.0 bb 1.0 MM 1.0 MM 1.0 MMI 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 115 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-10,, Name: FC3G_013S030 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:41:50 PFBA FC3G_013S030 6.12 100~l . ^ 6.57 %- 4.20 ^ v PFPeA FC3G013S030 100- 5.90 5.65. 6.73 4.29 v^V(`if,4.93 PFHxA FC3G 013S030 5.00 6.00 7.00 PFNA FC3G 013S030 5.00 6.00 7.00 PFDA FC3G_013S030 PFUnA FC3G_013S030 PFHxS-1 FC3G_013S030 PFHxS-2 FC3G_013S030 PFHxS-3 FC3G_013S030 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S030 PFDoA FC3G 013S030 PFOS-1 FC3G 013S030 PFOA-1 FC3G_013S030 100- 7.09 JT7-23e2 i 2910 %- \ 7.78 7.00 8.00 PFBS-1 FC3G..013S030 100--4.03 5'f 5 .77 ,5.90 %- 4.85 L t\7-21 !% A!\ 5.00 6.00 7.00 PFOS-2 FC3G_013S030 100- 8.29 1.19e4 45058 8.00 10.00 12.00 PFOA-2 FC3G 013S030 PFBS-2 FC3G 013S030 PFOSA FC3G_013S030 100-, 10.05 7.85e1 418 %8.61 q o-} t 9f 1i 1136H .75 , Jf 4 ' M flrf min 8.00 10.00 12.00 m Page 116 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-10,, Name: FC3GJJ13S030 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:41:50 13C2-PFOA FC3G 013S030 13C8-PFOA FC3G 013S030 13C4-PFOA FC3G_013S030 100- 7.09 7.50e4 296161 %- 1802-PFHxS-2 FC3G_013S030 100- 7.29 5.85o3 23858 %- 13C4-PFBA FC3G 013S030 7.00 8.00 13C2-PFHxA FC3G013S030 100- 6.34 6.31 e4 282955 %- 7.00 8.00 13C8-PFOSA FC3G_013S030 100- 9.96 9.54e3~| 50076 %- 13G2-PFOUEA FC3G 013S030 100--2 7.38 .92e4"l "109965 A %- 5.00 6.00 7.00 8.00 10.00 12.00 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G013S030 100- 8.29 r8.89e3 I 33822 %- 13C4-PFOS-2 FC3G013S030 100- 8.29 i 7.62e3 27937 %- 1802-PFHXS-1 FC3G 013S030 -rp-r mm 8.00 10.00 12.00 8.00 10.00 12.00 13C5-PFNA FC3G013S030 100-1 7 ACL 7.07e4 283341 %- 7.00 t min 8.00 13C2-PFDA FC3G 013S030 100- ' 8.27e4 1 303512 I%- i 0- ^ ............. min 8.00 10.00 12.00 13C2-PFDoA FC3G013S030 100p 9.15 r 1.41es I 611461 %- 0: 8.00 10.00 12.00 m Page 117 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-10,, Name: FC3G_013S030 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:41:50 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-l 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFD0 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.28 27 262.98 >218.... 27 312.97> 268.98 6.34 27 362.97> 318.98 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 > 518.... 8.55 30 612.95 >568.... 9.15 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84> 98.96 7.46 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 10.05 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.33 21 402.9>103 7.29 21 216.99>171.98 5.17 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 349 772 723 154 493 9072 9625 6963 19 14184 11887 78 188317 66776 75036 8892 7623 8474 5852 23871 63063 70712 82665 140757 9536 29240 IS Area 23871 63063 63063 63063 188317 188317 70712 82665 82665 140757 8474 5852 8474 5852 8474 8892 7623 9536 75036 75036 75036 75036 75036 75036 75036 75036 75036 75036 75036 75036 ng/g 0.012 0.083 0.839 0.820 0.314 22.018 25.687 38.953 7.054 4.794 18.357 19.303 17.828 18.511 15.384 17.003 14.195 12.414 12.808 9.269 5.120 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.1366 0.0635 0.0396 0.0146 0.0677 0.2562 0.0760 0.2293 0.0277 0.0576 0.0486 0.0810 0.0729 0.0179 %Rec S/N 6 11 3 2 2 102 182 113 1 109.3 118.7 80.7 103.0 108.3 100.0 103.9 129.5 143.1 119.5 104.5 107.8 78.0 103.4 288 509 15 1840 527 984 784 210 674 0 199 2094 750 713 522 508 858 Noise 232 280 268 137 837 295 730 316 236 272 31 33 117 63 5 174 89 27 404 493 301 43 133 48 0 468 135 378 426 1171 99 128 Height 1456 3049 2910 451 1692 32245 42946 30805 69 50045 45058 418 743648 259920 296161 33822 27937 32041 23858 92992 282955 283341 303512 611461 50076 109965 IS Height 92992 282955 282955 282955 743648 743648 283341 303512 303512 611461 32041 23858 32041 23858 32041 33822 27937 50076 296161 296161 296161 296161 296161 296161 296161 296161 296161 296161 296161 296161 Sample size 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 2.0200 Factor2 Detection... 1.0 MMI 1.0 1.0 bbl 1.0 1.0 MM 1.0 MM 1.0 bbl 1.0 bb 1.0 MM 1.0 MM 1.0 1.0 1.0 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 MMI 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 118 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-11,, Name: FC3G_013S031 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:58:51 PFBA FC3G_013S031 100- 6.25 5.17. 6.10. A m 6.49 % - 4.05 / VVy , (V 54)0'6^00'74)0 PFPeA FC3G_013S031 RQR-1 7Re9-7RR PFHxA FC3G 013S031 100- 6.31 6.61 e2 i 3714 %-: 4.07 6.10 ( ) ty V '/W 'l ^ 0- min PFNA FC3G_013S031 7 AO 100 ] 2.59e2^ 1041 * 8.02 l/ X J"i.i.i-i-t":", ............... min 7.00 8.00 PFDA FC3G 013S031 10 - f s ' i S s ! 30718 %" 0 i4r.'t.1TTrnTTm , inTTf? min 8.00 10.00 12.00 PFUnA FC3G_013S031 1001 8.55 7.22e3 I 30117 %- : 0~* 8.00 10.00 12.00 PFHxS-1 FC3G_013S031 PFHxS-2 FC3G_013S031 7.00 8.00 PFHxS-3 FC3G 013S031 100- 7.46 7:78 fJA\ l/\( / 8.02 %- 7.09 / \J l( V\ 0-\ / 7.00 \ 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S031 PFOA-1 FC3G 013S031 PFOA-2 FC3G 013S031 PFDoA FC3G 013S031 PFBS-1 FC3G 013S031 5.00 6.00 7.00 PFBS-2 FC3G 013S031 100- 4.03 7.11 \* Ifi ftII*il(fIif, 6.63 , \ y 5.00 6.00 7.00 PFOS-1 FC3G_013S031 100- 8.29 F 2.42e4 1 87760 1 1 ,,............. . , min 8.00 10.00 12.00 PFOS-2 FC3G 013S031 100- 8.29 T 2.03e4 if 78391 i %- 1 1! 0- - __r,,.ITTTT....rrTT min 8.00 10.00 12.00 PFOSA FC3G_013S031 100-, 9.96 1.57e2 I 886 I %- I 7.81 I 10.53 0~ jrr^ r rrJ ^ r v -.-rfTT-.-fT mill 8.00 10.00 12.00 m Page 119 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-11,, Name: FC3G_013S031 Description: 1,WG42409,15/4000uL Date: 11^Jan-2013 Time: 20:58:51 13C2-PFOA FC3GJJ13S031 100- 7.09 JT 1.46e5 I 591076 % 13C8-PFOA FC3G_013S031 100--i 7.09 5.36e4 ! 208095 0 7.00 8.00 7.00 8.00 1802-PFHXS-2 FC3G 013S031 13C4-PFBA FC3G_013S031 100- 5.17 I 2.53e4 I 84917 % - 4.18 OATM 5.00 6.00 7.00 13C8-PFOSA FC3G013S031 GGC 100 7.53e3 44921 I 4 -...........i i 8.00 10.00 min 12.00 13C2-PFOUEA FC3G013S031 100^1 7.38 2.74e4 1 1063731 %- - 0-^ 7.00 lilw 8.00 13C4-PFOA FC3G_013S031 100- 7.09 f 5.65e4 ( 227290 %- 7.00 8.00 13C2-PFHxA FC3G_013S031 100- 6.33 5.23e4 250761 % 5.00 6.00 7.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S031 13C5-PFNA FC3G 013S031 13C4-PFOS-2 FC3G 013S031 100- 8.29 F 6.35e3 1 24640 %- 1802-PFHXS-1 FC3G 013S031 100 7.29 7.36e3 ; ; 30290 %- 0 - 1 1 ' min 8.00 10.00 12.00 0 7.00 8.00 13C2-PFDA FC3GJ313S031 100 7.99 6.85e4 246337 %- 13C2-PFDOA FC3G__013S031 100 9.15 9.98e4 436770 8.00 10.00 12.00 8.00 10.00 12.00 m Page 120 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qid Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-11,, Name: FC3G_013S031 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 20:58:51 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 27 262.98 > 218.... 5.95 27 312.97> 268.98 6.31 27 362.97> 318.98 19 412.97> 368.98 7.05 19 412.97> 168.80 7.14 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 > 518.... 8.55 30 612.95 >568.... 9.15 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 >79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 21 402.9>103 7.29 7.29 21 216.99>171.98 5.17 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 173 661 422 113 259 8104 7216 4805 24248 20335 157 145941 53626 56505 7457 6347 7358 5121 25274 52259 57617 68523 99834 7529 27351 IS Area 25274 52259 52259 52259 145941 145941 57617 68523 68523 99834 7358 5121 7358 5121 7358 7457 6347 7529 56505 56505 56505 56505 56505 56505 56505 56505 56505 56505 56505 56505 ng/g 0.034 0.074 0.895 0.710 0.287 43.915 51.732 0.066 38.931 7.306 3.506 19.854 20.727 19.962 20.891 21.005 18.171 14.916 13.271 11.715 9.439 4.651 PV by LHE, SV by KJC 31-Jan-13. Note: Caiver = ng abs. SPM = ng/mL. LOD 0.0247 0.2115 0.0028 0.0229 0.0435 0.1822 0.2201 0.0502 0.1601 1.8124 0.0502 0.0244 0.0401 0.0535 0.1077 0.0112 %Rec S/N 3 17 3 1 2 150 102 118 112.5 126.6 60.8 114.7 119.8 115.3 120.7 182.0 157.5 129.3 115.0 101.5 81.8 96.7 997 453 22 , 41912 922 242 309 273 1221 374 29 1296 1888 888 714 390 1244 Noise 291 226 215 253 405 344 577 205 295 190 34 25 266 49 52 88 173 41 14 226 940 92 90 25 53 2924 194 126 277 611 115 86 Height 785 3714 1314 426 1041 30718 30117 22501 87760 78391 886 591076 208095 227290 28384 24640 30290 19704 84917 250761 238472 246337 436770 44921 106373 IS Height Sample size 84917 2.0800 250761 2.0800 250761 2.0800 250761 2.0800 591076 2.0800 591076 2.0800 238472 2.0800 246337 2.0800 246337 2.0800 436770 2.0800 30290 2.0800 19704 2.0800 30290 2.0800 19704 2.0800 30290 2.0800 28384 2.0800 24640 2.0800 44921 2.0800 227290 2.0800 227290 2.0800 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 227290 2.0800 2.0800 Factor2 Detection... 1.0 1.0 bb 1.0 MMI 1.0 1.0 MMI 1.0 MM 1.0 bbl 1.0 MM 1.0 MM 1.0 MM 1.0 1.0 1.0 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 121 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G :\Masslynx\lnst_G\PFC.PRO\FC\FC3G__013-B .qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-12,, Name: FC3G_013S032 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 21:15:52 PFBA FC3G_013S032 100- !!, 6.18 % 4.20 ! >#i A r 6'5l7.21 V 0- min 5.00 6.00 7.00 PFPeA FC3G_013S032 100- 25.0848e2~], 6.73 913 rt ,,6.92 k ij'ii'i 'V -in v 0" 5.00 6.00 7.00 PFHxA FC3G013S032 100- 6.29 8.34e2 3779 %- 6.10 4.57 H .vWV\ /-V-A 5.00 6.00 7.00 PFNA FC3G 013S032 100- 7.55 5.86e2 - 1818 . %: 5.63 ^ 7 .0 5 / 0J 7.00 t min 8.00 PFDA FC3G_013S032 T100- 7.99 5.21e3 jj_ 18690 %- 0: i L . 11A7, t min 8.00 10.00 12.00 PFUnA FC3G 013S032 : |Fi100- 8.52 4.82e3 19155 %i 0- J i . 1056 . 8.00 10.00 12.00 PFHxS-1 FC3G 013S032 PFHxS-2 FC3G_013S032 PFHxS-3 FC3G_013S032 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S032 Tn- min PFOA-1 FC3G_013S032 100- 7.09 \ 7.41 e2 \ 2188 %- 8.20 7.00 8.00 PFOA-2 FC3G 013S032 PFDoA FC3G_013S032 100- 9.12 f 3.59e3 I 15320 %- PFBS-1 FC3G 013S032 PFBS-2 FC3G 013S032 , . 1100;.5n9p-rrrjTT I 8.00 10.00 12.00 PFOS-1 FC3G 013S032 100 8.29 F3.45e3 i 12665 %I o:-A w 8.00 10.00 min 12.00 PFOS-2 FC3G 013S032 100- 8.29 2.76e3 I1 10399 %- m km 0- .rrn , ^ min 8.00 10.00 12.00 PFOSA FC3G 013S032 100- 9.93 9.83e1~1 568 %- 7.70 / \ 0 - -a a .". ' /10.74 8.00 10.00 12.00 m Page 122 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\RFC.PRO\FC\FC3G__013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-12,, Name: FC3G_013S032 Description: 1,WG42409,15/4000uL Date: 11Jan-2013 Time: 21:15:52 13C2-PFOA FC3G013S032 100-j 7.09 2.04e5 810887 13C8-PFOA FC3G 013S032 100- 7.09 IT 7.79e4 296062 %- 7.00 , -T-r min 8.00 1802-PFHxS-2 FC3G013S032 100- 7.29 i 7.05e3 30047 %- qJ 7.00 13C4-PFBA FC3G 013S032 ; 8.00 13C4-PFOA FC3G_013S032 100- 7.05 I " 8.02e4 314777 %- 7.00 8.00 13C2-PFHxA FC3G 013S032 7.00 8.00 13C8-PFOSA FC3G 013S032 100- 9.96 6.43e3 40418 ; o - , _____ 4 8.00 10.00 min 12.00 13C2-PFOUEA FC3G013S032 100n 7.33 2.97e4 (109881 %- 07.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S032 fi QQ 100-- ! 1.06e4 40587 ; : L a -____________ min 8.00 10.00 12.00 13C5-PFNA FC3G 013S032 100- 7.46 7.09e4 ~k 292943 I %- 07.00 i min 8.00 13C4-PFOS-2 FC3G 013S032 100- i " 6.67e3 1 23094 %- 0 - 1A 1 .......... .. min 8.00 10.00 12.00 13C2-PFDA FC3G 013S032 100- 7.99 f 9.12e4 336205 %1a m 0 8.00 .................. min 10.00 12.00 1802-PFHXS-1 FC3G_013S032 100q 7.29 A 9.11e3 i \ 35468 %r 0-1 7.00 8.00 > 1 ,1oo 13C2-PFDOA FC3G_013S032 9.15 1.17e5 494743 %- :\ (H -------8.00 10.00 12.00 m Page 123 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G__013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-12,, Name: FC3G_013S032 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 21:15:52 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.17 27 262.98 >218.... 5.88 27 312.97> 268.98 6.29 27 362.97> 318.98 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.55 29 512.96 >468.... 7.99 29 562.96 > 518.... 8.52 30 612.95 >568.... 9.12 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79 .96 7.33 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.93 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 37 1.9 7.05 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.21 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.96 458.98 >393.98 7.33 Area 754 204 834 741 234 586 5205 4819 . 3590 122 3451 2760 98 204485 77907 80215 10580 6666 9114 7053 25466 62831 70871 91217 116772 6429 29725 IS Area 25466 62831 62831 62831 204485 204485 70871 91217 91217 116772 9114 7053 9114 7053 9114 10580 6666 6429 80215 80215 80215 80215 80215 80215 80215 80215 80215 80215 80215 80215 ng/g 0.033 0.004 0.127 0.006 0.315 0.300 0.060 0.150 4.123 6.292 0.007 38.425 7.477 4.978 19.842 15.335 17.419 20.267 14.909 15.390 12.924 12.444 9.653 5.677 5.055 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. ' LOD 0.0085 0.0312 0.0242 0.0040 0.0231 0.0828 0.3226 0.1145 0.2280 0.3046 0.0222 0.0115 0.0222 0.0337 0.0301 0.0036 %Rec S/N 7 6 12 5 0 4 108 103 126 8 111.0 129.6 86.3 114.6 88.6 100.6 117.1 129.2 133.4 112.0 107.9 83.7 49.2 105.1 179 98 20 4823 5374 645 703 126 453 289 147 2385 3563 1580 929 907 4216 Noise 322 159 315 263 450 0 493 174 186 122 32 19 50 0 44 71 107 29 168 55 488 58 183 78 104 681 119 82 213 533 45 26 Height 2352 913 3779 2188 770 1818 18690 19155 15320 403 12665 10399 568 810887 296062 314777 40587 23094 35468 30047 100126 282673 292943 336205 494743 40418 109881 IS Height Sample size 100126 2.0800 282673 2.0800 282673 2.0800 282673 2.0800 810887 2.0800 810887 2.0800 292943 2.0800 336205 2.0800 336205 2.0800 494743 2.0800 35468 2.0800 30047 2.0800 35468 2.0800 30047 2.0800 35468 2.0800 40587 2.0800 23094 2.0800 40418 2.0800 314777 2.0800 314777 2.0800 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 2.0800 314777 314777 2.0800 2.0800 314777 2.0800 314777 2.0800 2.0800 Factor2 Detection.. 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 1.0 1.0 MM 1.0 1.0 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 124 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G__013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-13,, Name: FC3G_013S033 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 21:32:45 PFBA FC3G 013S033 PFPeA FC3GQ13S033 100- 5.99 6.77e2 3444 6.57 % - 5.77 _ ` l r 4.095.51 ! ,,/ , 7.16 0-V 5.00 6.00 7.00 PFHxA FC3G_013S033 100- 7.21 6.34 A %- 1.20e3 ] 6266 j\ 4.09 5.92. V i, 5.00 6.00 7.00 PFNA FC3G_013S033 1 ACi 100 ] 1.74e3 : 6738 6.90 7.00 \ -t-t min 8.00 PFDA FC3G_013S033 100- r 3.30e3 j< 10156 %- M }.....t -n 0J 8.00 11-1 11.99 f a. -a -'./v - ^ y > ................ irn-n I , i min 10.00 12.00 PFUnA FC3G013S033 1<XH 8.55 4.58e3 17414 %" L o- 8.00 10.00 12.26 12.00 PFHxS-1 FC3G_013S033 PFHxS-2 FC3G013S033 PFHxS-3 FC3G013S033 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S033 PFDoA FC3G 013S033 PFOA-1 FC3G_013S033 100' 7.09 A 1.22e3 . ' 4707 PFOA-2 FC3G 013S033 7.00 7.96 i . . . m in 8.00 PFBS-1 FC3G_013S033 PFBS-2 FC3G 013S033 PFOS-1 FC3G 013S033 PFOS-2 FC3G 013S033 PFOSA FC3G 013S033 m Page 125 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-13,, Name: FC3G_013S033 Description: 1,WG42409,15/4000uL Date: 11^Jan-2013 Time: 21:32:45 13C2-PFOA FC3GJ313S033 100 '.'2.0465 .795762 13C8-PFOA FC3G 013S033 8.67e4 338988 %{ 13C4-PFOA FC3G013S033 ioo-q 7.09 . 8.61e4 339908 %: 7.00 I - n min 8.00 07.00 -r-r min 8.00 0: j I 7.00 8.00 1802-PFHxS-2 FC3G_013S033 T QQ 1 0 0 -]6 .2 4 e 3 25444 . 7.00 min 8.00 13C4-PFBA FC3G 013S033 100%- 2.90e4 1 96551 y 4.13 0 - WSWrfmTTTmTTVT min 5.00 6.00 7.00 13C2-PFHxA FC3G 013S033 100- 6.21 e 4 l 305628 %- o5.00 6.00 7.00 13C8-PFOSA FC3G 013S033 100- 9.99 9 .4 5 e 3 l 50155 J 13C2-PFOUEA FC3G 013S033 100- 7.43 3.92e4 _147311 %: 08.00 I min 10.00 12.00 mMIk 0J 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S033 100- 8.29 8.53e3 29933 0 -----.... 8.00 10.00 12.00 13C5-PFNA FC3G 013S033 13C4-PFOS-2 FC3G013S033 100-, 8.29 f 7.43e3 I 27026 %- 8.00 10.00 12.00 13C2-PFDA FC3G_013S033 100- 7.99 F 8.70e4 I 314678 %- 8.00 10.00 12.00 1802-PFHxS-1 FC3G_013S033 100 7.33 -9 .54e 3l " 38038 1 %- . ...,- r - 7.00 8.00 13C2-PFDOA FC3G_013S033 100 9.15 F 1.51e5 I 647094 % 0-+T 8.00 10.00 12.00 m Page 126 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-13,, Name: FC3G_013S033 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 21:32:45 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.23 27 262.98 >218.... 5.99 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 28 462.96 >418.... 7.49 29 512.96 >468.... 7.99 29 562.96 >518.... 8.55 30 612.95 > 568.... 9.15 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84 98.96 7.29 24 398.84> 118.70 22 498.93 79.96 8.29 23 498.93 98.96 8.29 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 37 1.9 7.09 21 503 > 79.96 8.29 21 503 98.96 8.29 21 402.9>84 7.33 21 402.9103 7.33 21 216.99>171,98 5.19 21 314.97>269.98 6.36 21 467.96422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.15 21 506>78 9.99 458.98 393.98 7.43 Area 759 677 1201 746 1216 1743 3301 4583 2706 42 4021 2951 197 203619 86741 86068 8526 7429 9537 6241 29036 62135 70010 87044 150877 9451 39188 IS Area 29036 62135 62135 62135 203619 203619 70010 87044 87044 150877 9537 6241 9537 6241 9537 8526 7429 9451 86068 86068 86068 86068 86068 86068 86068 86068 86068 86068 86068 86068 ng/g 0.146 0.025 0.075 0.163 0.125 0.279 5.717 5.638 0.063 33.411 7.269 5.004 13.962 14.922 15.916 15.661 14.843 13.289 11.148 10.370 10.891 7.288 6.243 PV by LFIE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0160 0.0317 0.0061 0.0352 0.0940 0.0979 0.0200 0.1760 0.6860 0.0438 0.0334 0.0218 0.0410 0.0331 0.0533 %Rec S/N 4 15 21 13 25 10 50 50 55 1 103.0 134.5 92.6 86.1 92.0 98.2 96.6 137.3 122.9 103.1 95.9 100.7 67.4 138.6 107 99 57 3133 3548 427 396 421 2413 276 55 1135 999 1307 865 888 351 Noise 697 227 305 257 188 548 701 202 348 200 27 22 109 114 87 127 109 19 254 96 797 76 64 16 92 1766 269 276 241 748 56 419 Height 2764 3444 6266 3444 4707 6738 10156 17414 11059 149 13539 10805 1094 795762 338988 339908 29933 27026 38038 25444 96551 305628 275578 314678 647094 50155 147311 IS Height Sample size 96551 2.2200 305628 2.2200 305628 2.2200 305628 2.2200 795762 2.2200 795762 2.2200 275578 2.2200 314678 2.2200 314678 2.2200 647094 2.2200 38038 2.2200 25444 2.2200 38038 2.2200 25444 2.2200 38038 2.2200 29933 2.2200 27026 2.2200 50155 2.2200 339908 2.2200 339908 2.2200 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 2.2200 339908 339908 2.2200 2.2200 339908 2.2200 2.2200 Factor2 Detection. 1.0 MMI 1.0 MM 1.0 MMI 1.0 bb 1.0 MM 1.0 MM-I 1.0 MM 1.0 MM 1.0 MM 1.0 MMI 1.0 1.0 1.0 1.0 MMI 1.0 1.0 MM 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 127 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: L19064-14,, Name: FC3G_013S034 Description: 1,WG42409,15/4000uL Date: 11^Jan-2013 Time: 21:49:46 PFBA FC3G 013S034 PFPeA FC3G013S034 PFHxA FC3G Q13SQ34 PFNA FC3G_013S034 7 Aa 100 1 7.11e2 2429 . -6.63 -/ 7.00 min 8.00 PFDA FC3G 013S034 100I 1.19e4 41075 r %i! 0 min 8.00 10.00 12.00 PFUnA FC3G_013S034 loo-q 8.52 { 1.56e4 i 68837 %" - 0-1 8.00 10.00 12.00 PFHxS-1 FC3G 013S034 PFHxS-2 FC3G_013S034 PFHxS-3 FC3G 013S034 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G013S034 100- 6-68 6.68 3,10e2 ; 3.i0e2 1157 i l 1157 %- 4.85.77., - W PFOA-1 FC3G 013S034 ti...I-V-:...i i min 5.00 6.00 7.00 PFDoA FC3G013S034 100- q 16 f~1.38e4 I 61824 0/o/ -- PFBS-1 FC3G_013S034 1 0 0 - " 4-03 6-49 0 T1111r i 1 M 8.00 10.00 12.00 TT 5.00 6.00 7.00 PFOS-1 FC3G 013S034 PFOS-2 FC3G_013S034 mn 8 2 9 100n F 5.05e4 183992 %J PFOA-2 FC3G 013S034 PFBS-2 FC3G 013S034 PFOSA FC3G_013S034 100-, 9.96 1.73e3^) 9243 I %- 8.00 10.00 12:00 8.00 10.00 12.00 IO Page 128 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3GJ313-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-14,, Name: FC3G_013S034 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 21:49:46 13C2-PFOA FC3G 013S034 7.09 2.00e5 794915 oo 07.00 . t t min 8.00 13C8-PFOA FC3G 013S034 100- 7.09 f 8.26e4 323223 %: | | | l o7.00 8.00 1802-PFHxS-2 FC3G_013S034 100- 7.33 _ G.53e3, 23715/' i %- 13C4-PFBA FC3G_013S034 100- 5.21 3.08c4 H1i 103299 %- 4.03 7.00 8.00 13C8-PFOSA FC3G_013S034 100- 9.96 1.13e4 j 61673 ' %- 5.00 6.00 7.00 13C2-PFOUEA FC3G013S034 100- 7.38 3.43e4 .. '127907 H %- JllbJ 8.00 10.00 12.00 7.00 8.00 13C4-PFOA FC3G_013S034 100- 7.09 ,C 8.17e4 i \ 323569 %- 0 -i^ U ! .. ..................min 7.00 8.00 13C2-PFHxA FC3G 013S034 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G013S034 100- 8.29 9.68e3 36627 %- 13C4-PFOS-2 FC3G_013S034 100- _ 8.29 7.60e3 29426 %' 1802-PFHXS-1 FC3G 013S034 8.00 10.00 12.00 8.00 10.00 12.00 13C5-PFNA FC3G_013S034 7 AQ 100-1 7.81c4 308838 %- 13C2-PFDA FC3G 013S034 100- F 8.81 e4 S 331279 %Z 13C2-PFDoA FC3G013S034 100-, 9.15 1.65e5 719824 %- 7.00 -i min 8.00 0J ! ' min 8.00 10.00 12.00 0-* -fT'TT-H^Tr-p^ min 8.00 10.00 12.00 m Page 129 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-14,, Name: FC3G_013S034 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 21:49:46 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.28 27 262.98 >218.... 5.93 27 312.97> 268.98 6.38 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 > 468.... 7.99 29 562.96 > 518.... 8.52 30 612.95 > 568.... 9.15 24 298.94> 79.96 6.49 25 298.94 > 98.95 24 398.84 > 79.96 7.29 25 398.84> 98.96 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.33 21 216.99>171.98 5.21 21 314.97>269.98 6.33 21 467.96>422.97 7.49 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 373 276 1141 310 1283 294 711 11877 15601 13836 25 217 63604 50518 1728 200132 82573 81741 9676 7598 9545 6527 30842 70376 78108 88052 165435 11302 34271 IS Area 30842 70376 70376 70376 200132 200132 78108 88052 88052 165435 9545 6527 9545 6527 9545 9676 7598 11302 81741 81741 81741 81741 81741 81741 81741 81741 81741 81741 81741 .81741 ng/g 0.046 0.097 0.178 0.015 1.084 1.309 0.773 0.281 91.742 111.053 1.525 38.001 8.008 5.223 18.337 17.660 18.435 18.954 18.245 17.418 14.393 12.139 13.819 10.085 6.001 LOO 0.0049 0.1764 0.0843 0.0241 0.0217 0.0879 0.0740 0.0668 0.0425 0.1364 0.0347 0.0193 0.0340 0.0472 0.0154 0.0090 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 7 6 18 3 32 27 7 149 401 330 7 4 106.6 134.8 87.9 102.9 99.1 103,4 106.4 153.6 146.6 121.1 102.2 116.3 84.9 121.2 2311 1307 281 1358 986 721 599 701 819 1228 340 1743 2237 1018 965 2708 2007 Noise 214 157 238 361 142 53 338 276 172 187 24 38 186 0 92 98 141 33 586 328 449 61 42 46 19 304 185 138 326 746 23 64 Height 1455 973 4242 1157 4614 1452 2429 41075 68837 61824 162 723 225572 183992 9243 794915 323223 323569 36627 29426 37380 23715 103299 322463 308838 331279 719824 61673 127907 IS Height Sample size 103299 2.0200 322463 2.0200 322463 2.0200 322463 2.0200 794915 2.0200 794915 2.0200 308838 2.0200 331279 2.0200 331279 2.0200 719824 2.0200 37380 2.0200 23715 2.0200 37380 2.0200 23715 2.0200 37380 2.0200 36627 2.0200 29426 2.0200 61673 2.0200 323569 2.0200 323569 2.0200 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 323569 2.0200 2.0200 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 MMI 1.0 MMI 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MMI 1.0 1.0 MM 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 130 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-103,,DUP Name: FC3G_013S035 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:06:47 PFBA FC3G_013S035 100% - 4.18 6-1.6.33 M J L-6.53 5.00 6.00 7.00 PFNA FC3G_013S035 7 >1Q 100 6.13e2 1937 7.00 PFPeA FC3G 013S035 PFQA FC3G_013S035 100- F 1.31e4 [i 48883 % - |! PFHxA FC3G 013S035 100- 9 .6 0 e 2 l 4945 I %: 5.19 6-12J \ 7.26 / 05.00 6.00 7.00 PFUnA FC3G__013S035 lo o n ` 8.52 1.54e4 68198 %: 7.00 PFHxS-1 FC3G 013S035 min 8.00 0^ 8.00 rr- r r min 10.00 12.00 PFHxS-2 FC3G 013S035 8.00 10.00 12.00 PFHxS-3 FC3G 013S035 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S035 Rt 100 7.48e2 2929 ; ; 4.03 O. lo ......'I ' .... ...... ..... mm 5.00 6.00 7.00 PFDoA FC3G_013S035 100- 1.41e4 : 62406 ; i; L-- -- -tt min 8.00 1C.00 12.00 PFOS-1 FC3G_013S035 100 8.29 i 6.20e4 219219 % PFOA-1 FC3G 013S035 100- %- / V7.55 ........ \ 07.00 8.00 min PFBS-1 FC3G 013S035 100- , 6.68 5.95 In 5.06 %- 4.48 I I SIli 1I o- \ i : 1 / mmilnn 5.00 6.00 7.00 PFOS-2 FC3G 013S035 PFOA-2 FC3G013S035 100- 7.55 %J o7.00 8.00 PFBS-2 FC3G__013S035 6.44 ioo-q %- 6.44 2.72e1 2 .7 2 e ll 225 225 I / I 4.70 6 .2 5 1 6 .68 kk lili o- L 5.00 6.00 7.00 PFOSA FC3G_013S035 100-1 9.96 1.97e3 10371 % 8.00 10.00 12.00 0~Vt 8.00 i... 10.00 12.00 m Page 131 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-103,,DUP Name: FC3G_013S035 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:06:47 13C2-PFOA FC3G 013S035 100- 7.09 1.91e5 TA>' 14 %- 13C8-PFOA FC3G013S035 100- 7.09 "~6.93e4 269858 %: 13C4-PFOA FC3G_Q13SQ35 100q 7.09 { 7.68e4 1303316 %J 07.00 _r_ m;n 8.00 0: 7.00 -r-r min 8.00 07.00 8.00 1802-PFHxS-2 FC3G_013S035 100n 1 7.29 6.65e3 I ]26546 0/o/ _ 7.00 8.00 13C4-PFBA FC3G_013S035 100- 5.17 2.57e4 97083 %4.03 b^^.Tyn-riTW'i-rTttt min 5.00 6.00 7.00 13C2-PFHxA FC3G 013S035 100- 6.34 6 .7 8 e 4 l 315223 1 %- 05.00 6.00 7.00 13C8-PFOSA FC3G 013S035 13C2-PFOUEA FC3G013S035 100-- 3 7.38 .22e 411 ` 122009 %- 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Caiver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S035 100q 8.29 7.66e3 27018 %- 13C4-PFOS-2 FC3G_013S035 100- 8.29 7.25e3 ; 26607 %- 1802-PFHxS-l FC3G_013S035 100-j 7.29 1 9.9603 140262 % M . ......... 8.00 10.00 12.00 1 .. 8.00 10'00 12.00 I1.11 7.00 8.00 13C5-PFNA FC3GJD13S035 7 AQ 100 1 7.01e4"| 274346 1 ;. \ 7.00 -.'fri-!-1 1, min 8.00 13C2-PFDA FC3G 013S035 100- F 8.36e4 1 300934 % - fl fri. i o " f f l ^ r r n m ................ min 8.00 10.00 12.00 13C2-PFDOA FC3G_013S035 100 9.12 %- 0 J ' i ' ' 1 1 , ' 111 min 8.00 10.00 12.00 C Page 132 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-103,,DUP Name: FC3G_013S035 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:06:47 # Name :: T y 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHXS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA y ; .;' ts# Trace RT 26 212.99>168.98 5.17 27 262.98 > 218. .. 5.99 27 312.97> 268.98 6.34 27 362.97 318.98 6.68 19 412.97 368.98 7.05 19 412.97 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 > 468.... 7.99 29 562.96 > 518.... 8.52 30 612.95 568.... 9.12 24 298.94 79.96 25 298.94 98.95 6.44 24 398.84 > 79.96 25 398.84 98.96 24 398.84 118.70 22 498.93 79.96 8.29 23 498.93 98.96 8.29 31 498 78 9.96 21 414.97 369.... 7.09 21 420.97 375.... 7.09 416.97 3 71.9 7.09 21 503 > 79.96 8.29 21 503 98.96 8.29 21 402.9>84 7.29 21 402.9103 7.29 21 216.99171.98 5.17 21 314.97269.98 6.34 21 467.96>422.97 7.49 21 514.96 469.96 7.99 21 614.95 > 569.... 9.12 21 506>78 9.96 458.98 393.98 7.38 Area 691 72 960 748 582 98 613 13087 15446 14132 27 61996 48495 1969 190874 69322 76841 7662 7252 9957 6653 25742 67841 70055 83649 142202 10768 32202 IS Area 25742 67841 67841 67841 190874 190874 70055 83649 83649 142202 9957 6653 9957 6653 9957 7662 7252 10768 76841 76841 76841 76841 76841 76841 76841 76841 76841 76841 76841 76841 ng/g 0.017 0.034 0.010 1.178 1.246 0.896 102.826 101.620 1.688 35.081 6.507 4.467 14.054 16.317 18.614 18.700 14.740 16.252 12.495 11.162 11.497 9.300 5.130 LOD 0.2817 0.1109 0.0587 0.0167 0.0691 0.0531 0.1016 0.0777 0.0214 0.2798 0.0473 0.0781 0.0128 0.0475 0.0447 0.0072 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 9 5 18 10 17 1 6 191 200 178 9 108.2 120.4 82.6 86.7 100.6 114,8 115.3 136.3 150.3 115.6 103.2 106.3 86.0 113.9 766 706 302 1734 1156 194 709 448 737 2652 151 1214 476 2380 807 867 2127 Noise 287 133 279 306 91 577 334 256 342 351 53 25 296 114 38 286 242 34 421 233 1563 38 59 55 10 643 260 576 126 772 68 57 Height 2620 699 4945 2929 1534 483 1937 48883 68198 62406 225 219219 171040 10371 729114 269858 303316 27018 26607 40262 26546 97083 315223 274346 300934 623280 58997 122009 IS Helght Sample size 97083 2.2200 315223 2.2200 315223 2.2200 315223 729114 2.2200 2.2200 729114 2.2200 274346 2.2200 300934 2.2200 300934 2.2200 623280 2.2200 40262 2.2200 26546 2.2200 40262 2.2200 26546 2.2200 40262 2.2200 27018 26607 58997 303316 303316 2.2200 2.2200 2.2200 2.2200 2.2200 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 303316 303316 2.2200 2.2200 303316 303316 2.2200 2.2200 303316 2.2200 303316 2.2200 303316 2.2200 2.2200 Factor2 Detection... 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM 1.0 bbl 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 1.0 MMI 1.0 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 133 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-15,, Name: FC3G_013S036 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:23:48 PFBA FC3G 013S036 PFPeA FC3G 013S036 100- 6.57 5.93 j\ 4.44 5.00 k I \ \k jfp k ^ 5.00 6.00 7.00 PFNA FC3G_013S036 7 AQ 100 1 8.47e2 2814 7.00 , --------- K ' ' ' I ' 11' I ' ' ' ` I ' 7.00 8.00 PFHxS-1 FC3G 013S036 PFDA FC3G 013S036 100- f " 1.68e4 j'l 64032 %-_ ji !! 0 T\Trr;i ,rn r r ir r 8.00 10.00 12.00 PFHxS-2 FC3G 013S036 PFHxA FC3G_013S036 100- 6.33 1.02e3 4288 %4.03 I 6.10 J7.11 W ,A y v w v ^ 5.00 6.00 7.00 PFUnA FC3G_013S036 100q 8.52 (" 9.11e3 1 37264 %: - 8.00 Tpr min 10.00 12.00 PFHxS-3 FC3G_013S036 100- 7 '24 %- :s,63 0 -^ 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S036 PFDoA FC3G 013S036 PFOA-1 FC3G_013S036 100- 7.09 6.69e2 : 2550 : %\ 7.67 0 7.00 8.00 PFBS-1 FC3G 013S036 PFOA-2 FC3G 013S036 PFBS-2 FC3G 013S036 PFOS-1 FC3G 013S036 PFOS-2 FC3G_013S036 100- 8.29 4.23e4 153081 ; MtJrr r r ,l |,' '' l l " ~ min 8.00 10.00 12.00 PFOSA FC3G_013S036 100-1 9.99 8.98e2 fi 3917 I %- 8.00 10.00 12.00 ice* Page 134 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\l nst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-15,, Name: FC3G_013S036 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:23:48 13C2-PFOA FC3G 013S036 13C8-PFOA FC3GJ313S036 100- 7.09 8.62e4 339609 %- 13C4-PFOA FC3G013S036 100- 7.09 JT 8.85e4 I 347243 %- 7.00 8.00 0-%=*. 7.00 8.00 1802-PFHxS-2 FC3G_013S036 7' 100 1 |~7.11e3 ' 29389 7.00 - , . min 8.00 13C4-PFBA FC3G 013S036 100- 2.51e4 ' 86623 %4.05 0J tit min 5.00 6.00 7.00 13C2-PFHxA FC3G_013S036 100q 6.33 6.85e4 331308 %- . 0-* 5.00 6.00 7.00 13C8-PFOSA FC3G013S036 100 9.99 5 .0 3 e 3 l 23326 J %- 13C2-PFOUEA FC3G 013S036 100 7.38 3.74e4 7 - 143278 . -r min 8.00 10.00 12.00 i8 0- 7.00 ^ min 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S036 100- 8.29 S~~6.15e3 1 23651 ; %- 13C4-PFOS-2 FC3GJ313S036 100-1 8.29 F 5.35e3 ~ I 19725 %- 0 - ____________ min 8.00 10.00 12.00 08.00 10.00 12.00 13C5-PFNA FC3GJD13S036 100- 7.46 8.04e4" | 337018 %- 13C2-PFDA FC3G_013S036 7 qq 100- C 8.42e4 1 328926 %- 7.00 8.00 Jr, 8.00 10.00 12.00 1802-PFHxS-1 FC3G013S036 100- 7.29 ;. 9.84e3 1 39297 % 7.00 8.00 13C2-PFDOA FC3G_013S036 100- 9.12 6.17e4 265231 % l L mm 8.00 10.00 12.00 m Page 135 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-15,, Name: FC3G_013S036 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:23:48 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.21 27 262.98 > 218.... 27 312.97> 268.98 6.33 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.05 28 462.96 > 418.... 7.49 29 512.96 >468.... 7.99 29 562.96 >518.... 8.52 30 612.95 >568.... 9.12 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.29 25 398.84> 98.96 7.29 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 >375.... 7.09 416.97 > 37 1.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 21 402,9>103 7.29 7.29 21 216.99>171.98 5.17 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.12 21 506>78 9.99 458.98 >393.98 7.38 Area 460 1020 330 669 219 847 16777 9106 5604 64 25 157 112 54584 42348 898 226109 86209 88540 6150 5348 9843 7114 25057 68538 80398 84208 61724 5025 37391 IS Area 25057 68538 68538 68538 226109 226109 80398 84208 84208 61724 9843 7114 9843 7114 9843 6150 5348 5025 88540 88540 88540 88540 88540 88540 88540 88540 88540 88540 88540 88540 ng/g 0.084 0.024 1.378 0.606 0.700 0.025 0.153 99.784 106.506 1.457 31.899 6.212 4.553 8.659 9.236 14.123 15.348 11.013 12.603 11.007 8.625 3.831 3.332 5.269 LOD 0.0609 0.0106 0.0459 0.0007 0.0198 0.0317 0.0019 0.0283 0.0529 0.1387 0.0177 0.1390 0.0260 0.0257 0.0319 0.0328 0.0200 0.0049 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 8 15 5 6 3 11 276 116 206 8 10 8 4 111.2 129.9 95.2 60.4 64.4 98.5 107.0 115.2 131.8 115.1 90.2 40.1 34.8 132.2 1064 796 107 3035 10116 483 481 188 2440 0 210 1789 1375 808 384 592 3246 Noise 240 192 294 210 439 229 264 232 322 109 67 29 80 81 0 182 192 37 293 34 718 49 105 16 0 413 185 245 407 690 39 44 Height 1947 4288 1002 2550 773 2814 64032 37264 22536 569 294 659 354 194025 153081 3917 890381 339609 347243 23651 19725 39297 29389 86623 331308 337018 328926 265231 23326 143278 IS Height Sample size 86623 2.5100 331308 2.5100 331308 2.5100 331308 2.5100 890381 2.5100 890381 2.5100 337018 2.5100 328926 2.5100 328926 2.5100 265231 2.5100 39297 2.5100 29389 2.5100 39297 2.5100 29389 2.5100 39297 2.5100 23651 2.5100 19725 2.5100 23326 2.5100 347243 2.5100 347243 2.5100 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 347243 2.5100 2,5100 Factor2 Detection... 1.0 MMI 1.0 1.0 MMI 1.0 MMI 1.0 MMI 1.0 MM 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 MM 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb Page 136 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-16,, Name: FC3G_013S037 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:40:41 PFBA FC3G 013S037 100 (5.26;5.11e2;1990 4.09 6.31 6.87 5.00 6.00 7.00 PFPeA FC3G_013S037 , Qri 100-, c qn 2.38e2 2:3 f 1179 S/1179 %- 4.82 6.53 ,,7.11 pm 5.00 6.00 7.00 PFHxA FC3G_013S037 100n 6.31 8.82o2 . 3664 I %H4.03 ' 7 fi 5.90 .aA/7 ^ 5.00 6.00 7.00 PFNA FC3G 013S037 100- 7.55 3.73e27\ 1331 % - 6.76 8.20 _ 0: 7.00 i i i i - min 8.00 PFDA FC3G 013S037 100- 7.99 r2.69e3 % - | 8484 . 0-*lr pUn im8T'9m4, T11i;iN8^iM K 2mm. 8.00 10.00 12.00 PFUnA FC3G 013S037 100- 8.55 f 2.73e3 _ 1 10506 %" o: 8.00 o Z\ C0 --IP 12.14 10.00 12.00 PFHxS-1 FC3G 013S037 PFHxS-2 FC3G_013S037 PFHxS-3 FC3G 013S037 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3GJJ13S037 "loo- 4 7 8 6.73 4.03 5f . A 7.26 V 4 -87, 1 J V n Vis 5.00 6.00 7.00 PFDoA FC3G 013S037 PFOA-1 FC3G_013S037 100 7.05 f8 .4 9 e 2 I 2690 . 7.00 7.72 8.00 PFBS-1 FC3G 013S037 PFOA-2 FC3G 013S037 PFBS-2 FC3G_013S037 PFOS-1 FC3G 013S037 PFOS-2 FC3G 013S037 100- 8.29 P 9.57e2 I 2989 %- I f t 9.03 08.00 10.00 12.14 min 12.00 PFOSA FC3G 9.90 i0 0 n 6.65e1 : 384 %- 8.44 11.34 o - '''I*" 8.00 10.00 12.00 10 Page 137 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Massiynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-16,, Name: FC3G_013S037 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:40:41 13C2-PFOA FC3G_013S037 100- 7.09 1.98e5 1 757539 %- l 13C8-PFOA FC3G_013S037 100n 7.09 7.41 e4 | 286801 %- 13C4-PFOA FC3G_013S037 100- 7.09 C 8.31e4 320936 %- 7.00 8.00 1802-PFHxS-2 FC3G_013S037 100-1 I ^ .5 ie 3 26847 1 - -- | i min 7.00 8.00 13C8-PFOSA FC3G_013S037 100- 9.96 7.24e3 : 44032 S %- 7.00 8.00 13C4-PFBA FC3G 013S037 10 - 2.43e4 1 92954 %4.16 0 min 5.00 6.00 7.00 13C2-PFOUEA FC3G_013S037 100- 7.38 2.66e4 :102015/ \ %- o-VR 7.00 8.00 13C2-PFHxA FC3GJJ13S037 6.34 1001 ; %: 0^ 5.00 6.00 7.00 8.00 10.00 12.00 7.00 8 00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G013S037 100- 8.29 r 9q.08e3 34951 8.00 10.00 12.00 13C5-PFNA FC3G 013S037 100- 7.46 7.17c4 . 289170 ; ' %J 7.00 8.00 . 13C4-PFOS-2 FC3G 013S037 100- 8.29 f 8.69e3 I 35006 %Z 1802-PFHxS-1 FC3G 013S037 100- 7.29 \ 9.82o3 ,40523 %: 0- . iO l . -TTTT min 8.00 10.00 12.00 CH^ . . .. 7.00 8.00 13C2-PFDA FC3G 013S037 7.99 100- f 7.60e4 274055 %z 13C2-PFDoA FC3G013S037 100q 9.15 1.25e5 543049 %- 8.00 10.00 12.00 8.00 10.00 12.00 m Page 138 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PR0\FC\FC3G..013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: L19064-16,, Name: FC3G_013S037 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 22:40:41 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 RFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-l 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218. .. 5.90 27 312.97> 268.98 6.31 27 362.97> 318.98 19 412.97> 368.98 7.05 19 412.97> 168.80 28 462.96 >418.... 7.55 29 512.96 >468.... 7.99 29 562.96 >518.... 8.55 30 612.95 > 568.... 9.15 24 298.94> 79.96 25 298.94 > 98.95 24 398.84 > 79.96 25 398.84> 98.96 7.33 24 398.84> 118.70 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.90 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.19 21 314.97>269.98 6.34 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 >569.... 9.15 21 506>78 9.96 458.98 >393.98 7.38 Area 511 238 882 849 373 2688 2726 3292 15 1295 957 66 197796 74067 83109 9083 8694 9824 6578 24324 60341 71728 75992 125157 7245 26583 IS Area 24324 60341 60341 60341 197796 197796 71728 75992 75992 125157 9824 6578 9824 6578 9824 9083 8694 7245 83109 83109 83109 83109 83109 83109 83109 83109 83109 83109 83109 83109 ng/g 0.045 0.026 0.112 0.170 0.002 1.657 1.368 36.577 6.995 5.258 16.763 19.682 18.477 18.602 14.014 14.545 12.872 10.202 10.181 6.296 4.609 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0158 0.0052 0.0016 0.0486 0.0693 0.1933 0.0898 0.2814 0.0328 0.0041 0.0198 0.0475 0.0392 0.0109 %Rec S/N 6 6 16 8 5 39 45 77 3 103.6 118.9 89.4 95.0 111.5 104.7 105.4 119.1 123.6 109.4 86.7 86.5 53.5 94.0 47 32 24 20870 13288 325 723 318 659 0 148 1541 9793 1442 723 759 1273 Noise 353 192 224 164 319 81 244 215 235 201 56 22 140 32 0 85 92 16 36 22 988 48 110 61 0 629 175 30 190 751 58 80 Height 1990 1179 3664 2690 1331 8484 10506 15431 108 4011 2989 384 757539 286801 320936 34951 35006 40523 26847 92954 269675 289170 274055 543049 44032 102015 IS Height Sample size 92954 2.0400 269675 2.0400 269675 2.0400 269675 2.0400 757539 2.0400 757539 2.0400 289170 2.0400 274055 2.0400 274055 2.0400 543049 2.0400 40523 2.0400 26847 2.0400 40523 2.0400 26847 2.0400 40523 2.0400 34951 2.0400 35006 2.0400 44032 2.0400 320936 2.0400 320936 2.0400 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 2.0400 320936 320936 320936 2.0400 2.0400 2.0400 320936 2.0400 2.0400 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 1.0 bbl 1.0 MM 1.0 bb 1.0 MM 1.0 1.0 1.0 1.0 MMI 1.0 1.0 MM 1.0 bb 1.0 MMI 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 139 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-101,,BLK Name: FC3G_013S020 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 17:52:20 PFBA FC3G 013S020 PFPeA FC3G 013S020 PFNA FC3G 013S020 PFHxS-1 FC3G 013S020 PFDA FC3G_013S020 100- 8.05 A n-11e3 3948 %- 12.26 11.25. L i v'WwV' 8.00 10.00 12.00 PFHxS-2 FC3G_013S020 100- 7.33 _ 13e1 S41 A %- 6.71 8.08 7.00 8.00 PFHxA FC3G_013S020 100 96.3-376e 2 l 4789 j) %- 4.80 5.06 I r-vA 5.00 6.00 7.00 PFUnA FC3G013S020 100 _ 8.55 1.93e2 12.02 1064 J^ %- A 10.77^1 j\, |lW -AWA wj............ 8.00 10.00 12.00 PFHxS-3 FC3G 013S020 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G 013S020 100- 3.68 6.64e2 i. 2397 % 5.54 6 40 : 0 ^ -rrn 1 n i i | r . - T Tr r n 7 rr rr Tn r mm min 5.00 6.00 7.00 PFOA-1 FC3G 013S020 100- 7.09 V"7.09e2 \ 2235 %Z \ 7.84___ U0 -^ .n,.-.T...T, T, ,r r..r..n.1-...i.r..,.T., : min 7.00 8.00 PFOA-2 FC3G_013S020 100- 7.14 :> 1.90e2 775 %- 6.631 7.00 8.00 PFDoA FC3G_013S020 100 %~ 7.87 9.21 4.11e2 1697 11.99 10.59 I PFBS-1 PFBS-2 0- 8.00 10.00 12.00 PFOS-1 FC3G_013S020 8.23 3.29e2 12.23 778 11.72 8.00 10.00 12.00 PFOS-2 FC3G_013S020 100- 11.07^11,60 11.96 9.36 %- 8.29 i Ili V A Ili 8.00 10.00 12.00 PFOSA FC3G_013S020 m Page 140 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnstJ3\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-101,,BLK Name: FC3G_013S020 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 17:52:20 13C2-PFOA FC3G_013S020 100 1 2.10e5 844623 13C8-PFOA FC3G 013S020 100- ' 6.58e4 262742 %z 13C4-PFOA FC3G013S020 100q ' 7.09 I" 8.09e4 .314376 %- 7.00 J,___ -TT min 8.00 0- t47.00 - n min 8.00 0: 7.00 8.00 1802-PFHxS-2 FC3G 013S020 13C4-PFBA FC3G 013S020 13C2-PFHxA FC3G_013S020 <nn 6.34 _ 7.07e4 339817 13C8-PFOSA FC3G_013S020 100- 9.99 1.14641 55488 13C2-PFOUEA FC3G..013S020 100~ 7.43 / 54e4l 96663, %- 8.00 10.00 12.00 7.00 8.00 5.00 6.00 7.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G_013S020 100- (f 1.09e4 j| 43024 13C4-PFOS-2 FC3G 013S020 100- 8.50e3 31304 %c 1802-PFHXS-1 FC3G013S020 i0 0 n 7.33 1.08e4 A 44845 1 %- -rrrr min 8.00 10.00 12.00 4. oC - , ................. min 8.00 10.00 12.00 0-1 7.00 8.00 13C5-PFNA FC3G_013S020 7 AQ 100 6.94e4_| 277702 yfTH-i min 7.00 8.00 13C2-PFDA FC3G 013S020 100 6.66e4 1 234801 %- 0- L 8.00 , 10.00 , min 12.00 13C2-PFDoA FC3G_013S020 100q 9.18 T 1.07e5 I 493306 %- 0-* 8.00 10.00 12.00 IC Page 141 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-101,,BLK Name: FC3G_013S020 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 17:52:20 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-l 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.28 27 262.98 >218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.14 28 462.96 > 418.... 29 512.96 >468.... 8.05 29 562.96 >518.... 8.55 30 612.95 >568.... 9.21 24 298.94> 79.96 25 298.94 > 98.95 6.38 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 22 498.93> 79.96 8.23 23 498.93> 98.96 31 498 > 78 21 414.97 >369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.26 21 314.97>269.98 6.34 21 467.96>422.97 7.49 21 514.96 >469.96 8.05 21 614.95 >569.... 9.18 21 506>78 9.99 458.98 >393.98 7.43 Area 670 166 937 664 709 190 1112 193 411 10 46 65 329 210191 65827 80937 10934 8497 10836 7816 32061 70653 69441 66623 106551 11409 25359 IS Area 32061 70653 70653 70653 210191 210191 69441 66623 66623 106551 10836 7816 10836 7816 10836 10934 8497 11409 80937 80937 80937 80937 80937 80937 80937 80937 80937 80937 80937 80937 ng/g 0.020 0.005 0.096 0.033 0.099 40.710 6.512 5.223 21.136 20.147 21.346 23.150 19.347 17.837 13.052 9.368 9.078 10.384 4.485 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0259 0.0314 0.1137 0.0044 0.0231 0.1374 0.1672 0.0493 0.3331 0.2437 0.0219 0.0100 0.0499 0.0450 0.0268 0.0468 %Rec S/N 7 3 21 12 13 6 10 5 7 3 42 3 7 113.1 108.5 87.1 117.4 111.9 118.6 128.6 161.2 148.6 108.8 78.1 75.7 86.5 89.7 1112 4574 678 468 343 1384 211 234 3024 4042 511 722 1457 287 Noise 286 308 226 192 169 131 288 382 203 258 36 , 23 4 102 17 111 159 7 760 57 463 92 91 32 146 523 112 69 460 684 38 336 Height 2046 807 4789 2397 2235 775 3948 1064 1697 80 179 341 778 844623 262742 314376 43024 31304 44845 30769 122462 339817 277702 234801 493306 55488 96663 IS Height 122462 339817 339817 339817 844623 844623 277702 234801 234801 493306 44845 30769 44845 30769 44845 43024 31304 55488 314376 314376 314376 314376 314376 314376 314376 314376 314376 314376 314376 314376 Sample size 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 2.0000 Factor2 Detection... 1.0 MMI 1.0 MM 1.0 bbl 1.0 MM 1.0 bbl 1.0 MM 1.0 1.0 MMI 1.0 MMI 1.0 MMI 1.0 1.0 MMI 1.0 MM 1.0 MMI 1.0 1.0 MM 1.0 1.0 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 142 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-102,,SPM Name: FC3G_013S017 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 17:01:25 PFBA FC3G_013S017 100- F 4.51 e4 1 174050 %- PFPeA FC3G 013S017 100- 6 ^ 1 282976 PFHxA FC3G013S017 100-q 6.36 I.21e5 ] - 576976 L ................. _ n* min 5.00 6.00 7.00 0 - Trrrr min 5.00 6.00 7.00 05.00 6.00 7.00 PFNA FC3G 013S017 100- 7.55 1.37e5 514423 %- , o- 7.00 min 8.00 PFDA FC3G 013S017 100-| f 8.05 1.76e5 ! 628490 s% y 0 IK 8.00 10.00 min 12.00 PFUnA FC3G_013S017 100^ 8.58 T 2.06e5 I 904322 %: 0J 8.00 10.00 12.00 PFHxS-1 FC3G 013S017 100n 7.33 2 99e4 - 112285 %- uo- ..................... t min 7.00 8.00 PFHxS-2 FC3G 013S017 1 0 0- 7.33 1.68&a_?l I!62536 %- _ 0 7.00 -T-T min 8.00 PFHxS-3 FC3G 013S017 100- 7.33 9.97e3~* - 37443 . , %- 0U - -- 7.00 min 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S017 100-, fi 1.19e5 462166 %- I i min 5.00 6.00 7.00 PFOA-1 FC3G 013S017 J|~ 1.29e5 I; m 499382 % X 0 1/' ri-T-Vt i , -,4t m i . min 7.00 8.00 PFOA-2 FC3G013S017 7.14 10 ] i " 2.97e4 113690 1 ' 07.00 8.00 PFDoA FC3G 013S017 100- 9.21 2.27e5 902977 %: !l : 8.00 10.00 12.00 PFBS-1 FC3G 013S017 100- 6.47 3.25e4 144504 i %: " 111" " !1' " i" " r ` : 5.00 6.00 7.00 PFBS-2 FC3G013S017 100- 6.47 1.7764 82457 % ________J \ V 5.00 6.00 7.00 PFOS-1 FC3G 013S017 PFOS-2 FC3G_013S017 100- _ 8.35 1.79e4 57492 %- PFOSA FC3G_013S017 100- 9.99 2.44e4 88663 %- i '"'"'T7 min 8.00 10.00 12.00 IX 8.00 10.00 12.00 ][ Page 143 of 234 .axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-102,,SPM Name: FC3G_013S017 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 17:01:25 13C2-PFOA FC3G_013S017 100- 7.09 JT2.46e5 I ',980226 %- 13C8-PFOA FC3G 013S017 100- 7.09 8.29e4 332713 %- 13C4-PFOA FC3G_013S017 100- 7.09 i 8.77e4 11334357 %- 7.00 8.00 7.00 8.00 7.00 8.00 1802-PFHxS-2 FC3G_013S017 7 -JO 100 l7.87e3 ' 32631 ' 7.00 -T min 8.00 13C4-PFBA FC3G 013S017 lo o n ' 2.64e4 99528 %: 4.03 \ 0 -1 ,uTT^rrfrrrrrr min 5.00 6.00 7.00 13C2-PFHxA FC3G_013S017 io n 6.36 6.51e4 305374 %: - o: 5.00 6.00 7.00 13C8-PFOSA FC3G_013S017 100- 9.99 1.55e4l 79247 l 13C2-PFOUEA FC3G 013S017 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S017 100-] 8.35 f 1-24*4 1 49463 %I c k min 8.00 10.00 12.00 13C4-PFOS-2 FC3G 013S017 100-1 8.35 I 9.24e3 j 36537 i 4 0 - min- J - ------------------------------------------------------8.00 10.00 12.00 1802-PFHxS-1 FC3G 013S017 100- 7.33 1.09e4~Jk 43281 1 %- 0j 7.00 T -m 8.00 13C5-PFNA FC3G_013S017 ' -,100 7.55 9 .1 9 e 4 l 360761 %- A 7.00 ir p ~.i i 1 min 8.00 13C2-PFDA FC3G_013S017 100-, 8.05 1.01e5 1 I 372440 : Ik 8.00 10.00 12.00 13C2-PFDoA FC3G 013S017 m Page 144 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: WG42409-102,,SPM Name: FC3G_013S017 Description: 1,WG42409,15/4000uL Date: 11-Jan-2013 Time: 17:01:25 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDOA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.23 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 >418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 >518.... 8.58 30 612.95 >568.... 9.21 24 298.94> 79.96 6.47 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 >371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.19 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 >569.... 9.21 21 506>78 9.99 458.98 >393.98 7.43 Area 45106 61531 120536 118685 128693 29652 137069 176239 206279 226758 32543 17750 29932 16817 9968 29013 17866 24373 246349 82938 87669 12387 9238 10919 7871 26386 65120 91883 100764 151683 15475 28591 IS Area 26386 65120 65120 65120 246349 246349 91883 100764 100764 151683 10919 7871 10919 7871 10919 12387 9238 15475 87669 87669 87669 87669 87669 87669 87669 87669 87669 87669 87669 87669 ng/g 34.796 31.412 35.989 31.644 34.765 35.441 31.093 34.017 33.039 39.920 78.469 75.827 74.487 73.172 75.695 65.612 64.577 34.765 88.100 15.148 11.315 44.213 40.445 39.717 43.047 29.398 30.356 31.889 26.162 23.863 26.008 10.112 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0641 0.0121 0.0322 0.0862 0.0051 0.0459 0.2677 0.1600 0.0176 0.6565 0.0323 0.0534 0.0912 0.1337 0.0463 0.0539 %Rec 87.0 78.5 90.0 79.1 86.9 88.6 77.7 85.0 82.6 99.8 98.1 94.8 93.1 91.5 94.6 82.0 80.7 86.9 122.4 126.2 94.3 122.8 112.3 110.3 119.6 122.5 126.5 132.9 109.0 99.4 108.4 101.1 S/N Noise 771 226 899 315 3025 191 1685 274 2420 206 1389 82 665 773 1645 382 3835 236 2636 343 2180 66 4477 18 2569 44 370 169 00 531 161 481 119 1756 . 51 3199 306 9455 . 35 739 452 519 95 786 46 7043 6 00 133 749 3464 88 1843 196 834 447 593 2260 1080 35 563 192 Height 174050 282976 576976 462166 499382 113690 514423 628490 904322 902977 144504 82457 112285 62536 37443 85506 57492 88663 980226 332713 334357 49463 36537 43281 32631 99528 305374 360761 372440 640853 79247 108208 IS Height Sample size 99528 1.0000 305374 1.0000 305374 1.0000 305374 1.0000 980226 1.0000 980226 1.0000 360761 1.0000 372440 1.0000 372440 1.0000 640853 1.0000 43281 1.0000 32631 1.0000 43281 1.0000 32631 1.0000 43281 1.0000 49463 1.0000 36537 1.0000 79247 1.0000 334357 1.0000 334357 1.0000 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 334357 1.0000 1.0000 Factor2 Detection.., 1.0 bb 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1,0 bb 1.0 bb 1.0 bb Page 145 of 234 www.axysanalytical.com __________________ f l I Sample List Report P U y g T L ____________ ________________________ WlassLynx 4.1 SCN627 Sample List; G:\Masslynx\lnst_G\_Master.PRO\SampleDB\FC3G_013.SPL Last Modified: Friday, January 11,2013 13:01:07 Pacific Standard Time Printed: Friday, January 11,2013 13:05:15 Pacific Standard Time Page 1 of 1 Page Position (1,1) File Name Sample ID File Text Bottle Signfn SignOut Inj VoL 1 FC3G 013S001 MeOH,, 1,,15uL 2 FC3G 013S002 MeOH,, 1,,15uL 3 FC3G 013S003 FC-LIN-42151T-A,,1/2 1,,15uL 4 FC3G 013S004 FC-LIN-42151T-A,,1/2 1,,15uL 5 FC3G 013S005 FC-LIN-42151T-B,,1/2 1,,15uL 6 FC3G 013S006 FC-LIN-42151T-C,,1 /2 1,,15uL 7 F.C3G 013S007 FC-LlN-42151T-D,,1/2 1,,15uL 8 FC3G 013S008 FC-LIN-42151T-E,,1/2 1,,15uL 1:8,A 1:8,A 2:1,A 2:1,A 2:1,B 2:1,C 2:1,D 2:1,E L IU --- ------ M "i --- -, --- --- 15.0 15.0 15.0 15.0 -, 15.0 15.0 15.0 V 15.0 9 FC3G 013S009 FC-LIN-42151T-F,, 1/2 1,,1 5uL 10 FC3G 013S010 FC-LIN-42151T-G,, 1/2 1,,15uL 11 FC3G 013S011 FC-LIN-42151T-H.,1/2 1,,15uL 2:1,F 2:2,A 2:2,B ~J-- - --- -- 15.0. 15.0 15.0 0 12 FC3G 013S012 MeOH,, 1,,15uL 1:8,A - -- 15.0 13 FC3G 013S013 MeOH,, 1,,15uL 14 FC3G 013S014 FC-LIN-42151T-D,,1/2 1,,15uL 1:8,A 2:1,D - "Z 15.0 15.0 15 FC3G 013S015 FC-LiN-42151 T-D,,1/2 1,,15uL 2:1,D - -V 15.0 16 FC3G 013S016 WG42409-104,,SAR 1,WG42409,15/4000uL 3:4,A - 15.0 17 FC3G 013S017 WG42409-102,,SPM 18 FC3G 013S018 MeOH,, 19 FC3G 013S019 MeOH,, 20 FC3G 013S020 WG42409-101,,BLK 21 FC3G 013S021 L19064-1,, 1,WG42409,15/4000uL 1,,15uL 1,, 15uL ' 1,WG42409,15/4000uL 1,WG42409,15/4000uL 3:4,B 1:8,A 1:8,A 3:4,C 3:4,0 - "1 -\ - 15.0 15.0 15.0 \ 15.0 I 15.0 22 FC3G 013S022 L19064-2,,. 23 FC3G 013S023 L19064-3,, 1,WG42409,15/4000uL 3:4,E 1,WG42409,15/4000uL 3:4,F - -- -- 15.0 I 15.0 24 FC3G 013S024 L19064-4,, 25 FC3G 013S025 L19064-5,, 1,WG42409,15/4000uL 3:5,A 1,WG42409,15/4000uL 3:5,B - - -- -- 15.0 15.0 26 FC3G 013S026 L19064-6,, 27 FC3G 013S027 L19064-7,,. 28 FC3G 013S028 L19064-8,, 29 FC3G 013S029 L19064-9,, 30 FC3G 013S030 L19064-10,, 31 FC3G 013S031 L19064-11,, 32 FC3G 013S032 L19064-12,, 33 FC3G 013S033 L19064-13,, 1.WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 1,WG42409,15/4000uL 3:5,0 3:5,D 3:5,E 3:5,F 3:6,A 3:6, B 3:6,C 3:6,D --- - I* ' -_ --- --_ ~ -- . -,,,, -- -- 15.0 15.0 15.0 15.0 15.0 15.0 15.0 15.0 34 FC3G 013S034 L19064-14,, 1,WG42409,15/4000UL 3:6,E - - 35 FC3G 013S035 WG42409-103..DUP 1.WG42409,15/4000uL 3:6,F - - 36 FC3G 013S036 L19064-15,, 1,WG42409,15/4000uL 3:7,A -- 37 FC3G 013S037 L19064-16,, 1,WG42409,15/4000uL 3:7,B --- 38 FC3G 013S038 FC-LIN-42151T-DJ/2 1,,15uL 2:1,D - 39 FC3G 013S039 FC-LIN-42151T-DJ/2 1,, 15uL t2:1, D - 40 FC3G 013S040 MeOH,, 1,,15uL 1:8,A Y ~ -- - MM MM J/ -I 15.0 15,0 15.0 15.0 15.0 15.0 ~ 7 15.0 O ckzs I ivo WO-5 c ^LS> /?r Page 146 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 06 Nov 2012 13:32:38 Calibration: 14 Jan 2013 13:08:53 Compound name: PFBA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 5.24 5.24 5.26 5.26 5.24 5.24 5.21 5.26 Area 2349 4251 10366 45779 255608 529256 1106351 1451154 IS Area 23694 25135 22826 23689 24224 26173 21823 13033 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 Compound name: PFBA Correlation coefficient: r = 0.996181, rA2 = 0.992377 Calibration curve: 1.14415 * x + 1.21576 Response type: Internal Std ( Ref 26 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None ng/g 1.017 2.485 8.463 39.474 220.277 423.109 1062.375 2334.523 %Rec 101.7 99.4 84.6 98.7 110.1 105.8 106.2 93.4 S/N 38 32 189 460 124 1863 18584 4954 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 147 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFPeA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 5.99 6.01 5.99 5.99 5.97 5.97 5.95 5.97 Area 2008 5123 14550 67138 336833 733203 1451118 2412291 IS Area 64291 64250 58083 62914 55802 52550 43564 33387 Compound name: PFPeA Correlation coefficient: r = 0.993143, rA2 = 0.986334 Calibration curve: 0.721029 * x + 0.0279352 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.001 2.615 8.299 35.482 200.881 464.376 1108.718 2404.932 %Rec 100.1 104.6 83.0 88.7 100.4 116.1 110.9 96.2 S/N 37 195 596 1692 7323 10162 63240 30997 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 148 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.38 6.38 6.38 6.36 6.34 6.36 6.34 6.34 Area 4568 9975 27425 130876 610449 1180792 2301871 3564080 IS Area 64291 64250 58083 62914 55802 52550 43564 33387 Compound name: PFHxA Correlation coefficient: r = 0.995059, rA2 = 0.990142 Calibration curve: 1.22047 * x + 0.500033 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.987 2.643 8.875 40.497 214.713 441.448 1038.652 2098.793 %Rec 98.7 105.7 88.8 101.2 107.4 110.4 103.9 84.0 S/N 143 167 598 1704 6667 20565 39460 45309 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 149 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHpA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.73 6.78 6.73 6.73 6.73 6.78 6.73 6.73 Area 4308 9766 28576 146028 638000 1404129 2756213 4155998 IS Area 64291 64250 58083 62914 55802 52550 43564 33387 Compound name: PFHpA Correlation coefficient: r = 0.993371, rA2 = 0.986786 Calibration curve: 1.37563 * x + 0.211383 Response type: Internal Std ( Ref 27 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.015 2.498 8.430 40.341 199.318 466.012 1103.663 2171.579 %Rec 101.5 99.9 84.3 100.9 99.7 116.5 110.4 86.9 S/N 73 182 692 2276 10287 25207 73556 34263 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 150 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOA-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.14 7.14 7.14 7.09 Area 4305 10427 29956 146755 720547 1356679 2785893 4425141 IS Area 237751 239566 212071 256975 219864 218928 178447 132546 Compound name: PFOA-1 Correlation coefficient: r = 0.996840, rA2 = 0.993690 Calibration curve: 1.07468 * x + 0.251326 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.979 2.682 9.230 38.027 219.330 414.938 1045.711 2236.502 %Rec 97.9 107.3 92.3 95.1 109.7 103.7 104.6 89.5 S/N 123 362 487 6798 41534 25056 11748 9648 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 151 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOA-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.19 7.14 7.14 7.14 7.14 7.14 7.09 Area 869 2142 6254 33767 163932 315010 654423 1008404 IS Area 237751 239566 212071 256975 219864 218928 178447 132546 Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 Compound name: PFOA-2 Correlation coefficient: r = 0.995736, rA2 = 0.991490 Calibration curve: 0.244011 * x + 0.018253 Response type: Internal Std ( Ref 19 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None ng/g 1.004 2.563 8.626 38.698 219.931 424.491 1082.038 2244.799 %Rec 100.4 102.5 86.3 96.7 110.0 106.1 108.2 89.8 S/N 0 566 385 0 0 0 0 0 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 152 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFNA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.61 7.61 7.55 7.55 7.55 7.55 7.55 7.49 Area 4448 11290 32114 148161 791016 1497967 3206580 4992039 IS Area 81603 84569 67753 83045 74331 81811 64985 44026 Compound name: PFNA Correlation coefficient: r = 0.997754, rA2 = 0.995513 Calibration curve: 1.14558 * x + 0.183555 Response type: Internal Std ( Ref 28 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.982 2.637 9.770 37.217 222.788 383.440 1033.584 2375.365 %Rec 98.2 105.5 97.7 93.0 111.4 95.9 103.4 95.0 S/N 30 107 212 1221 3532 5263 13098 29400 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 153 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFDA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.11 8.11 8.05 8.05 8.05 8.05 8.05 8.05 Area 7175 15035 42575 190115 1042945 2117893 4358040 6485407 IS Area 101067 86238 84230 93912 96533 102880 98693 85123 Compound name: PFDA Correlation coefficient: r = 0.993688, rA2 = 0.987415 Calibration curve: 1.21718 * x + 0.571428 Response type: Internal Std ( Ref 29 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.930 2.968 9.497 39.447 212.560 405.438 870.216 1501.794 %Rec 93.0 118.7 95.0 98.6 106.3 101.4 87.0 60.1 S/N 153 159 465 1408 6927 8285 1730 10240 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MMX PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 154 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFUnA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.67 8.70 8.64 8.61 8.61 8.64 8.61 8.61 Area 7307 16832 47553 234886 1347697 2783679 6297988 9320023 IS Area 101067 86238 84230 93912 96533 102880 98693 85123 Compound name: PFUnA Correlation coefficient: r = 0.987103, rA2 = 0.974372 Calibration curve: 1.47655 * x + 0.347898 Response type: Internal Std ( Ref 29 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.940 2.937 8.941 40.418 226.688 439.560 1037.007 1779.414 %Rec 94.0 117.5 89.4 101.0 113.3 109.9 103.7 71.2 S/N 142 245 1332 4601 6793 21784 15999 19203 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 155 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFDoA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.27 9.30 9.27 9.24 9.24 9.27 9.24 9.24 Area 10194 22074 57475 286387 1640496 3481032 8082436 14006239 IS Area 169868 163299 142436 174079 191720 214405 246180 246757 Compound name: PFDoA Correlation coefficient: r = 0.980111, rA2 = 0.960617 Calibration curve: 0.883036 * x + 0.627837 Response type: Internal Std ( Ref 30 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.000 2.500 10.000 40.000 200.000 400.000 1000.000 2500.000 ng/g 0.920 2.963 10.256 44.003 231.852 440.561 891.613 1542.003 %Rec 92.0 118.5 102.6 110.0 115.9 110.1 89.2 61.7 S/N 226 490 876 3927 11958 4243 37235 81743 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 156 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFBS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.53 6.53 6.51 6.51 6.49 6.51 6.49 6.47 Area 1038 2683 7781 37888 178976 333040 616948 910658 IS Area 12951 13585 11291 13066 11125 9932 7971 5135 Compound name: PFBS-1 Correlation coefficient: r = 0.997410, rA2 = 0.994826 Calibration curve: 1.3654 * x + 0.148867 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 2.005 5.098 18.060 76.343 424.077 884.007 2040.651 4675.857 %Rec 100.2 102.0 90.3 95.4 106.0 110.5 102.0 93.5 S/N 127 187 768 9176 16927 47040 35111 31464 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 157 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFBS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.49 6.53 6.49 6.49 6.49 6.51 6.49 6.47 Area 682 1639 4065 20644 93640 176912 325842 464895 IS Area 8069 8402 7204 8344 6980 7888 5514 3683 Compound name: PFBS-2 Correlation coefficient: r = 0.994125, rA2 = 0.988285 Calibration curve: 1.0571 * x + 1.02602 Response type: Internal Std ( Ref 25 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.907 5.672 18.249 83.291 455.894 762.814 2011.315 4298.276 %Rec 95.3 113.4 91.2 104.1 114.0 95.4 100.6 86.0 S/N 89 193 514 3296 4810 15977 36917 27854 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 158 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.33 7.38 7.38 7.38 7.33 7.33 Area 995 2472 7449 35921 174989 321443 630934 875468 IS Area 12951 13585 11291 13066 11125 9932 7971 5135 Compound name: PFHxS-1 Correlation coefficient: r = 0.996495, rA2 = 0.993002 Calibration curve: 1.32396 * x + 0.0663729 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 2.040 4.897 17.890 74.702 427.668 879.991 2152.301 4635.939 %Rec 102.0 97.9 89.4 93.4 106.9 110.0 107.6 92.7 S/N 48 232 179 766 6713 3256 19148 4897 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 159 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.38 7.33 7.38 7.38 7.33 7.33 Area 583 1506 3685 19357 86452 183159 345834 486835 IS Area 8069 8402 7204 8344 6980 7888 5514 3683 Compound name: PFHxS-2 Correlation coefficient: r = 0.995281, rA2 = 0.990585 Calibration curve: 1.04313 * x + 0.587258 Response type: Internal Std ( Ref 25 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.929 5.624 17.091 79.505 426.879 800.785 2163.792 4561.884 %Rec 96.4 112.5 85.5 99.4 106.7 100.1 108.2 91.2 S/N 144 0 0 0 0 0 0 0 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 160 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFHxS-3 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.33 7.33 7.33 7.38 7.33 7.33 Area 401 953 2264 12138 56878 102356 208209 289888 IS Area 12951 13585 11291 13066 11125 9932 7971 5135 Compound name: PFHxS-3 Correlation coefficient: r = 0.994802, rA2 = 0.989632 Calibration curve: 0.430793 * x + 0.255689 Response type: Internal Std ( Ref 24 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.996 5.269 16.164 77.034 426.669 860.628 2182.301 4717.185 %Rec 99.8 105.4 80.8 96.3 106.7 107.6 109.1 94.3 S/N 0 63 0 0 0 0 77975 0 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 161 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.44 8.44 8.41 8.41 8.35 8.41 8.35 8.35 Area 1136 2550 6349 31778 164663 339460 744593 1072351 IS Area 12280 11889 10194 11195 11328 12244 9275 6391 Compound name: PFOS-1 Correlation coefficient: r = 0.995671, rA2 = 0.991361 Calibration curve: 1.2724 * x + 0.830661 Response type: Internal Std ( Ref 22 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.964 5.415 16.969 79.662 410.612 783.790 2270.578 4746.605 %Rec 98.2 108.3 84.8 99.6 102.7 98.0 113.5 94.9 S/N 34 62 108 355 1797 4253 4771 2634 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 162 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.44 8.44 8.41 8.41 8.41 8.41 8.35 8.35 Area 773 1784 4931 21613 108199 241234 502724 748686 IS Area 9118 9407 8158 10080 9161 9863 8107 5168 Compound name: PFOS-2 Correlation coefficient: r = 0.997851, rA2 = 0.995706 Calibration curve: 1.06261 * x + 0.999871 Response type: Internal Std ( Ref 23 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 2.000 5.000 20.000 80.000 400.000 800.000 2000.000 5000.000 ng/g 1.932 5.483 19.539 71.699 399.187 827.661 2099.973 4907.534 %Rec 96.6 109.7 97.7 89.6 99.8 103.5 105.0 98.2 S/N 46 59 176 745 1585 2892 5738 3303 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 163 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: PFOSA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.96 9.99 9.99 9.96 9.99 9.99 9.99 9.99 Area 1074 1891 5955 28377 177335 364640 935573 1753377 IS Area 15418 14099 14395 16141 18940 19590 19776 15345 Compound name: PFOSA Correlation coefficient: r = 0.997950, rA2 = 0.995904 Calibration curve: 1.07718 * x + 0.35077 Response type: Internal Std ( Ref 31 ), Area * ( IS Conc. / IS Area ) Curve type: Linear, Origin: Exclude, Weighting: 1/xA2, Axis trans: None Std. Conc 1.200 2.700 10.200 40.000 200.000 400.000 1000.000 2500.000 ng/g 1.226 2.663 8.891 38.844 208.283 414.386 1053.749 2545.530 %Rec 102.2 98.6 87.2 97.1 104.1 103.6 105.4 101.8 S/N 138 280 535 1329 5818 3875 9816 24758 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 164 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFOA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.14 7.14 7.09 7.09 Area 237751 239566 212071 256975 219864 218928 178447 132546 Compound name: 13C2-PFOA Response Factor: 0.382748 RRF SD: 0.0155651, % Relative SD: 4.06668 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 72.000 72.000 72.000 72.000 72.000 72.000 72.000 72.000 ng/g 67.507 70.606 72.985 68.946 71.975 74.028 73.284 76.671 %Rec 93.8 98.1 101.4 95.8 100.0 102.8 101.8 106.5 S/N 11115 2708 3477 1889 2754 3151 2103 2134 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 165 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C8-PFOA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.14 7.14 7.09 7.09 Area 86222 75200 68945 81521 72926 71423 57989 41290 Compound name: 13C8-PFOA Response Factor: 0.749417 RRF SD: 0.030168, % Relative SD: 4.02553 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/g 12.503 11.319 12.118 11.171 12.193 12.334 12.163 12.198 %Rec 104.2 94.3 101.0 93.1 101.6 102.8 101.4 101.7 S/N 1143 4719 2795 3552 4603 4024 3619 1227 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 166 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFOA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.14 7.14 7.14 7.14 7.09 7.14 7.09 7.09 Compound name: 13C4-PFOA Response Factor: 7747.83 RRF SD: 1680.24, % Relative SD: 21.6866 Response type: External Std, Area Curve type: Rf Area 110419 106379 91100 116856 95773 92721 76343 54200 IS Area Std. Conc 12.000 12.000 12.000 12.000 12.000 12.000 12.000 12.000 ng/g 14.252 13.730 11.758 15.082 12.361 11.967 9.853 6.996 %Rec 118.8 114.4 98.0 125.7 103.0 99.7 82.1 58.3 S/N 575 2948 115 308 392 1290 307 718 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 167 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFOS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.41 8.44 8.41 8.41 8.41 8.41 8.35 8.41 Area 12280 11889 10194 11195 11328 12244 9275 6391 Compound name: 13C4-PFOS-1 Response Factor: 0.0383506 RRF SD: 0.00346218, % Relative SD: 9.0277 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 34.798 34.971 35.015 29.976 37.010 41.318 38.017 36.896 %Rec 96.7 97.1 97.3 83.3 102.8 114.8 105.6 102.5 S/N 937 274 532 460 739 654 510 446 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 168 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFOS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.41 8.44 8.41 8.41 8.41 8.41 8.35 8.35 Area 9118 9407 8158 10080 9161 9863 8107 5168 Compound name: 13C4-PFOS-2 Response Factor: 0.0312656 RRF SD: 0.00294722, % Relative SD: 9.42639 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 31.694 33.939 34.368 33.108 36.713 40.828 40.756 36.593 %Rec 88.0 94.3 95.5 92.0 102.0 113.4 113.2 101.6 S/N 308 321 376 781 334 628 378 418 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 169 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 18O2-PFHxS-1 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.38 7.38 7.33 7.38 7.33 7.33 Area 12951 13585 11291 13066 11125 9932 7971 5135 Compound name: 18O2-PFHxS-1 Response Factor: 0.0376316 RRF SD: 0.00358002, % Relative SD: 9.51333 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 37.400 40.722 39.522 35.656 37.040 34.157 33.293 30.210 %Rec 103.9 113.1 109.8 99.0 102.9 94.9 92.5 83.9 S/N 990 1330 770 824 5201 1211 689 111 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 170 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 18O2-PFHxS-2 # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.38 7.38 7.38 7.38 7.33 7.38 7.33 7.33 Area 8069 8402 7204 8344 6980 7888 5514 3683 Compound name: 18O2-PFHxS-2 Response Factor: 0.0250277 RRF SD: 0.00183648, % Relative SD: 7.33779 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 36.000 36.000 36.000 36.000 36.000 36.000 36.000 36.000 ng/g 35.038 37.870 37.913 34.234 34.945 40.790 34.633 32.577 %Rec 97.3 105.2 105.3 95.1 97.1 113.3 96.2 90.5 S/N 255 0 2225 464 823 2137 1550 525 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 171 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C4-PFBA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 5.24 5.24 5.28 5.24 5.26 5.26 5.23 5.24 Area 23694 25135 22826 23689 24224 26173 21823 13033 Compound name: 13C4-PFBA Response Factor: 0.122853 RRF SD: 0.014587, % Relative SD: 11.8735 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 20.960 23.079 24.474 19.801 24.706 27.572 27.921 23.488 %Rec 87.3 96.2 102.0 82.5 102.9 114.9 116.3 97.9 S/N 110 88 130 98 115 230 129 56 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM bb MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 172 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFHxA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 6.36 6.38 6.36 6.36 6.34 6.36 6.34 6.33 Area 64291 64250 58083 62914 55802 52550 43564 33387 Compound name: 13C2-PFHxA Response Factor: 0.293638 RRF SD: 0.0155463, % Relative SD: 5.29437 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 23.794 24.682 26.055 22.002 23.811 23.162 23.320 25.174 %Rec 99.1 102.8 108.6 91.7 99.2 96.5 97.2 104.9 S/N 1775 2646 1262 1397 1189 1905 770 1475 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 173 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C5-PFNA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.55 7.61 7.55 7.55 7.55 7.55 7.55 7.49 Area 81603 84569 67753 83045 74331 81811 64985 44026 Compound name: 13C5-PFNA Response Factor: 0.394397 RRF SD: 0.029284, % Relative SD: 7.425 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 22.486 24.188 22.628 21.623 23.614 26.846 25.900 24.714 %Rec 93.7 100.8 94.3 90.1 98.4 111.9 107.9 103.0 S/N 1290 986 1626 2215 2591 2045 1284 1468 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 174 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFDA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 8.11 8.11 8.05 8.05 8.05 8.05 8.05 8.05 Area 101067 86238 84230 93912 96533 102880 98693 85123 Compound name: 13C2-PFDA Response Factor: 0.527187 RRF SD: 0.131866, % Relative SD: 25.0131 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 20.834 18.453 21.046 18.293 22.943 25.256 29.426 35.749 %Rec 86.8 76.9 87.7 76.2 95.6 105.2 122.6 149.0 S/N 862 1222 817 844 946 932 1190 597 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 175 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFDoA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.27 9.30 9.24 9.24 9.24 9.24 9.24 9.21 Area 169868 163299 142436 174079 191720 214405 246180 246757 Compound name: 13C2-PFDoA Response Factor: 0.870071 RRF SD: 0.169205, % Relative SD: 19.4472 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 21.217 21.172 21.564 20.546 27.609 31.892 44.474 62.790 %Rec 88.4 88.2 89.8 85.6 115.0 132.9 185.3 261.6 S/N 737 746 874 625 1044 1103 1399 2038 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MMX MMX PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 176 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C8-PFOSA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 9.96 9.99 9.96 9.96 9.96 9.99 9.99 9.99 Area 15418 14099 14395 16141 18940 19590 19776 15345 Compound name: 13C8-PFOSA Response Factor: 0.0814461 RRF SD: 0.0168185, % Relative SD: 20.6499 Response type: Internal Std ( Ref 21 ), Area * ( IS Conc. / IS Area ) Curve type: Rf IS Area 110419 106379 91100 116856 95773 92721 76343 54200 Std. Conc 24.000 24.000 24.000 24.000 24.000 24.000 24.000 24.000 ng/g 20.573 19.527 23.281 20.351 29.138 31.130 38.165 41.713 %Rec 85.7 81.4 97.0 84.8 121.4 129.7 159.0 173.8 S/N 1133 2203 1154 1646 1366 1835 2244 1157 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MMX MMX PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 177 of 234 www.axysanalytical.com Quantify Compound Summary Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Compound name: 13C2-PFOUEA # ID 1 FC-LIN-42151T-A,,1/2 2 FC-LIN-42151T-B,,1/2 3 FC-LIN-42151T-C,,1/2 4 FC-LIN-42151T-D,,1/2 5 FC-LIN-42151T-E,,1/2 6 FC-LIN-42151T-F,,1/2 7 FC-LIN-42151T-G,,1/2 8 FC-LIN-42151T-H,,1/2 Name FC3G_013S004 FC3G_013S005 FC3G_013S006 FC3G_013S007 FC3G_013S008 FC3G_013S009 FC3G_013S010 FC3G_013S011 Type Standard Standard Standard Standard Standard Standard Standard Standard RT 7.43 7.46 7.43 7.43 7.43 7.43 7.43 7.38 Compound name: 13C2-PFOUEA Response Factor: 2827.34 RRF SD: 398.089, % Relative SD: 14.08 Response type: External Std, Area Curve type: Rf Area 32596 29981 26306 30462 30315 30111 26510 19906 IS Area Std. Conc 10.000 10.000 10.000 10.000 10.000 10.000 10.000 10.000 ng/g 11.529 10.604 9.304 10.774 10.722 10.650 9.376 7.040 %Rec 115.3 106.0 93.0 107.7 107.2 106.5 93.8 70.4 S/N 2838 449 688 3001 656 2674 383 405 Acq.Date 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 11-Jan-13 A c q .T im e 13:20:41 13:37:42 13:54:43 14:11:44 14:28:37 14:45:38 15:02:39 15:19:32 Detection... MM MM MM MM MM MM MM MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 178 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 06 Nov 2012 13:32:38 Calibration: 14 Jan 2013 13:08:53 ID: FC-LIN-42151T-A,,1/2 Name: FC3G_013S004 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:20:41 PFBA FC3G_013S004 100 5.24 2.35e3 8535 %- min 5.00 6.00 7.00 PFPeA FC3G_013S004 100 5.99 2.01e3 8800 % min 5.00 6.00 7.00 PFHxA FC3G_013S004 100 6.38 4.57e3 23646 % 4.16 min 5.00 6.00 7.00 PFHpA FC3G 013S004 PFNA FC3G 013S004 PFDA FC3G 013S004 PFUnA FC3G 013S004 PFDoA FC3G 013S004 PFOA-1 FC3G 013S004 min PFBS-1 FC3G 013S004 PFOA-2 FC3G 013S004 min PFBS-2 FC3G 013S004 min PFHxS-1 FC3G 013S004 min PFHxS-2 FC3G 013S004 min min min min PFHxS-3 FC3G 013S004 100- 7.38 4.01e2 1359 PFOS-1 FC3G_013S004 100 8.44 1.14e3 3697 PFOS-2 FC3G_013S004 100 8.44 7.73e2 2525 PFOSA FC3G 013S004 %6.63 / %% 12.23 min 0 7.00 min 8.00 0 min 8.00 10.00 12.00 min 8.00 10.00 12.00 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 179 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-A,,1/2 Name: FC3G_013S004 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:20:41 13C2-PFOA FC3G 013S004 100- 7.14 2.38e5 963843 %- 13C8-PFOA FC3G 013S004 100- 7.14 8.62e4 339422 %- 13C4-PFOA FC3G_013S004 100 7.14 .10e5 433554 % 13C4-PFOS-1 FC3G 013S004 07.00 T-r min 8.00 07.00 t -t min 8.00 0 7.00 min 8.00 18O2-PFHxS-2 FC3G 013S004 13C4-PFBA FC3G 013S004 13C2-PFHxA FC3G 013S004 13C5-PFNA FC3G 013S004 13C4-PFOS-2 FC3G_013S004 100 8.41 9.12e3 38116 18O2-PFHxS-1 FC3G 013S004 min min 8.00 10.00 12.00 13C2-PFDA FC3G 013S004 13C2-PFDoA FC3G 013S004 min min min min min min min 13C8-PFOSA FC3G 013S004 13C2-PFOUEA FC3G 013S004 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 180 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-A,,1/2 Name: FC3G_013S004 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:20:41 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 5.99 27 312.97> 268.98 6.38 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.61 29 512.96 > 468.... 8.11 29 562.96 > 518.... 8.67 30 612.95 > 568.... 9.27 24 298.94> 79.96 6.53 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.38 22 498.93> 79.96 8.44 23 498.93> 98.96 8.44 31 498 > 78 9.96 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.24 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.11 21 614.95 > 569.... 9.27 21 506>78 9.96 458.98 >393.98 7.43 Area 2349 2008 4568 4308 4305 869 4448 7175 7307 10194 1038 682 995 583 401 1136 773 1074 237751 86222 110419 12280 9118 12951 8069 23694 64291 81603 101067 169868 15418 32596 IS Area 23694 64291 64291 64291 237751 237751 81603 101067 101067 169868 12951 8069 12951 8069 12951 12280 9118 15418 110419 110419 110419 110419 110419 110419 110419 110419 110419 110419 110419 110419 ng/g 1.017 1.001 0.987 1.015 0.979 1.004 0.982 0.930 0.940 0.920 2.005 1.907 2.040 1.929 1.996 1.964 1.932 1.226 67.507 12.503 14.252 34.798 31.694 37.400 35.038 20.960 23.794 22.486 20.834 21.217 20.573 11.529 LOD 0.0359 0.0550 0.0188 0.0329 0.0743 0.1198 0.3287 0.1178 0.4347 0.5994 0.0502 0.0500 0.0683 0.0960 0.0706 0.0122 %Rec 101.7 100.1 98.7 101.5 97.9 100.4 98.2 93.0 94.0 92.0 100.2 95.3 102.0 96.4 99.8 98.2 96.6 102.2 93.8 104.2 118.8 96.7 88.0 103.9 97.3 87.3 99.1 93.7 86.8 88.4 85.7 115.3 S/N 38 37 143 73 123 0 30 153 142 226 127 89 48 144 0 34 46 138 11115 1143 575 937 308 990 255 110 1775 1290 862 737 1133 2838 Noise 223 235 165 247 141 0 555 176 233 197 39 40 68 17 0 110 55 30 87 297 753 55 124 53 131 887 177 238 434 1006 69 45 Height 8535 8800 23646 18016 17345 2964 16904 26972 32984 44631 5003 3568 3250 2504 1359 3697 2525 4108 963843 339422 433554 51864 38116 52855 33361 97299 314894 306487 373861 742142 78447 129081 IS Height 97299 314894 314894 314894 963843 963843 306487 373861 373861 742142 52855 33361 52855 33361 52855 51864 38116 78447 433554 433554 433554 433554 433554 433554 433554 433554 433554 433554 433554 433554 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 181 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-B,,1/2 Name: FC3G_013S005 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:37:42 PFBA FC3G_013S005 100 5.24 4.25e3 15643 %- PFPeA FC3G 013S005 min 5.00 6.00 7.00 PFNA FC3G 013S005 PFDA FC3G 013S005 PFHxA FC3G 013S005 min PFUnA FC3G 013S005 PFHpA FC3G 013S005 min PFDoA FC3G 013S005 PFOA-1 FC3G 013S005 min PFBS-1 FC3G 013S005 PFOA-2 FC3G 013S005 min PFBS-2 FC3G 013S005 min PFHxS-1 FC3G 013S005 min PFHxS-2 FC3G 013S005 min min PFHxS-3 FC3G 013S005 min min min min PFOS-1 FC3G_013S005 100 8.44 2.55e3 7396 % PFOS-2 FC3G_013S005 100 8.44 1.78e3 6320 % PFOSA FC3G 013S005 12.02 min min min 8.00 10.00 12.00 8.00 10.00 12.00 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 182 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-B,,1/2 Name: FC3G_013S005 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:37:42 13C2-PFOA FC3G 013S005 100- 7.14 2.40e5 957565 %- 13C8-PFOA FC3G 013S005 100- 7.14 7.52e4 301233 %- 13C4-PFOA FC3G_013S005 100 7.14 1.06e5 1420901 % 13C4-PFOS-1 FC3G 013S005 0- ^4 7.00 T-r min 8.00 07.00 =rr min 8.00 0 7.00 min 8.00 18O2-PFHxS-2 FC3G 013S005 13C4-PFBA FC3G_013S005 100 5.24 2.51e4 103637 % 13C2-PFHxA FC3G 013S005 13C5-PFNA FC3G 013S005 13C8-PFOSA FC3G_013S005 100- 9.99 _ 1.41e4 74369 %- min min 5.00 6.00 7.00 13C2-PFOUEA FC3G 013S005 min 0 min 8.00 10.00 12.00 min 13C4-PFOS-2 FC3G 013S005 min 13C2-PFDA FC3G 013S005 min 18O2-PFHxS-1 FC3G 013S005 min 13C2-PFDoA FC3G 013S005 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 183 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-B,,1/2 Name: FC3G_013S005 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:37:42 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 6.01 27 312.97> 268.98 6.38 27 362.97> 318.98 6.78 19 412.97> 368.98 7.14 19 412.97> 168.80 7.19 28 462.96 > 418.... 7.61 29 512.96 > 468.... 8.11 29 562.96 > 518.... 8.70 30 612.95 > 568.... 9.30 24 298.94> 79.96 6.53 25 298.94 > 98.95 6.53 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.38 22 498.93> 79.96 8.44 23 498.93> 98.96 8.44 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.44 21 503 > 98.96 8.44 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.24 21 314.97>269.98 6.38 21 467.96>422.97 7.61 21 514.96 >469.96 8.11 21 614.95 > 569.... 9.30 21 506>78 9.99 458.98 >393.98 7.46 Area 4251 5123 9975 9766 10427 2142 11290 15035 16832 22074 2683 1639 2472 1506 953 2550 1784 1891 239566 75200 106379 11889 9407 13585 8402 25135 64250 84569 86238 163299 14099 29981 IS Area 25135 64250 64250 64250 239566 239566 84569 86238 86238 163299 13585 8402 13585 8402 13585 11889 9407 14099 106379 106379 106379 106379 106379 106379 106379 106379 106379 106379 106379 106379 ng/g 2.485 2.615 2.643 2.498 2.682 2.563 2.637 2.968 2.937 2.963 5.098 5.672 4.897 5.624 5.269 5.415 5.483 2.663 70.606 11.319 13.730 34.971 33.939 40.722 37.870 23.079 24.682 24.188 18.453 21.172 19.527 10.604 LOD 0.0036 0.0120 0.0790 0.0073 0.0140 0.4066 0.3551 0.0936 0.8207 0.0307 0.0695 0.0420 0.0931 0.0354 0.0709 %Rec 99.4 104.6 105.7 99.9 107.3 102.5 105.5 118.7 117.5 118.5 102.0 113.4 97.9 112.5 105.4 108.3 109.7 98.6 98.1 94.3 114.4 97.1 94.3 113.1 105.2 96.2 102.8 100.8 76.9 88.2 81.4 106.0 S/N 32 195 167 182 362 566 107 159 245 490 187 193 232 0 63 62 59 280 2708 4719 2948 274 321 1330 0 88 2646 986 1222 746 2203 449 Noise 484 118 248 209 109 15 404 344 299 199 61 36 42 0 54 119 106 28 354 64 143 182 130 41 0 1179 106 321 259 947 34 261 Height 15643 23136 41366 38052 39652 8737 43376 54598 73464 97770 11436 6968 9663 5226 3402 7396 6320 7858 957565 301233 420901 49991 41714 54779 34475 103637 279250 316249 315918 706856 74369 117336 IS Height 103637 279250 279250 279250 957565 957565 316249 315918 315918 706856 54779 34475 54779 34475 54779 49991 41714 74369 420901 420901 420901 420901 420901 420901 420901 420901 420901 420901 420901 420901 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 184 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-C,,1/2 Name: FC3G_013S006 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:54:43 PFBA FC3G 013S006 PFPeA FC3G 013S006 PFHxA FC3G 013S006 PFHpA FC3G 013S006 PFOA-1 FC3G 013S006 PFOA-2 FC3G 013S006 PFNA FC3G 013S006 PFHxS-1 FC3G 013S006 min PFDA FC3G 013S006 min PFHxS-2 FC3G 013S006 min PFUnA FC3G 013S006 min PFHxS-3 FC3G 013S006 min min PFDoA FC3G_013S006 100- 9.27 5.75e4 247552 %- PFBS-1 FC3G 013S006 min min 8.00 10.00 12.00 PFOS-1 FC3G 013S006 PFOS-2 FC3G 013S006 min PFBS-2 FC3G 013S006 min PFOSA FC3G 013S006 min min min min min min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 185 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-C,,1/2 Name: FC3G_013S006 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:54:43 13C2-PFOA FC3G 013S006 100- 7.14 2.12e5 829784 %- 13C8-PFOA FC3G 013S006 100- 7.14 6.89e4 265043 %- 13C4-PFOA FC3G_013S006 100 7.14 9.11e4 ,358242 % 13C4-PFOS-1 FC3G 013S006 07.00 T-r min 8.00 07.00 T-r min 8.00 0 7.00 min 8.00 18O2-PFHxS-2 FC3G 013S006 13C4-PFBA FC3G 013S006 13C2-PFHxA FC3G 013S006 13C5-PFNA FC3G 013S006 13C4-PFOS-2 FC3G 013S006 min 13C2-PFDA FC3G 013S006 18O2-PFHxS-1 FC3G 013S006 min 13C2-PFDoA FC3G 013S006 min min min min min min min 13C8-PFOSA FC3G_013S006 100- 9.96 1.44e4 71206 %- 13C2-PFOUEA FC3G 013S006 min 8.00 10.00 12.00 min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 186 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-C,,1/2 Name: FC3G_013S006 Description: 1,,15uL Date: 11-Jan-2013 Time: 13:54:43 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218.... 5.99 27 312.97> 268.98 6.38 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.64 30 612.95 > 568.... 9.27 24 298.94> 79.96 6.51 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.38 24 398.84> 118.70 7.33 22 498.93> 79.96 8.41 23 498.93> 98.96 8.41 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.28 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.96 458.98 >393.98 7.43 Area 10366 14550 27425 28576 29956 6254 32114 42575 47553 57475 7781 4065 7449 3685 2264 6349 4931 5955 212071 68945 91100 10194 8158 11291 7204 22826 58083 67753 84230 142436 14395 26306 IS Area 22826 58083 58083 58083 212071 212071 67753 84230 84230 142436 11291 7204 11291 7204 11291 10194 8158 14395 91100 91100 91100 91100 91100 91100 91100 91100 91100 91100 91100 91100 ng/g 8.463 8.299 8.875 8.430 9.230 8.626 9.770 9.497 8.941 10.256 18.060 18.249 17.890 17.091 16.164 16.969 19.539 8.891 72.985 12.118 11.758 35.015 34.368 39.522 37.913 24.474 26.055 22.628 21.046 21.564 23.281 9.304 LOD 0.0043 0.2197 0.0627 0.0127 0.3071 0.2073 0.2804 0.1568 0.0509 0.5747 0.0743 0.0379 0.0721 0.0790 0.0762 0.0405 %Rec 84.6 83.0 88.8 84.3 92.3 86.3 97.7 95.0 89.4 102.6 90.3 91.2 89.4 85.5 80.8 84.8 97.7 87.2 101.4 101.0 98.0 97.3 95.5 109.8 105.3 102.0 108.6 94.3 87.7 89.8 97.0 93.0 S/N 189 596 598 692 487 385 212 465 1332 876 768 514 179 0 0 108 176 535 3477 2795 115 532 376 770 2225 130 1262 1626 817 874 1154 688 Noise 213 118 208 169 246 58 564 329 162 283 47 36 150 0 0 178 98 41 239 95 3119 79 87 59 13 703 217 149 378 684 62 144 Height 40206 70334 124363 117134 120024 22218 119633 152978 216315 247552 35802 18662 26771 14483 8599 19312 17328 21789 829784 265043 358242 42111 32767 45248 28229 91558 273883 241529 308913 597777 71206 99136 IS Height 91558 273883 273883 273883 829784 829784 241529 308913 308913 597777 45248 28229 45248 28229 45248 42111 32767 71206 358242 358242 358242 358242 358242 358242 358242 358242 358242 358242 358242 358242 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 187 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S007 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:11:44 PFBA FC3G 013S007 PFPeA FC3G 013S007 PFHxA FC3G 013S007 PFHpA FC3G 013S007 PFOA-1 FC3G 013S007 PFOA-2 FC3G 013S007 PFNA FC3G 013S007 min PFDA FC3G 013S007 min PFUnA FC3G 013S007 min PFDoA FC3G 013S007 min PFBS-1 FC3G 013S007 min PFBS-2 FC3G 013S007 min PFHxS-1 FC3G 013S007 min PFHxS-2 FC3G 013S007 min PFHxS-3 FC3G 013S007 min PFOS-1 FC3G 013S007 min PFOS-2 FC3G 013S007 min PFOSA FC3G 013S007 min min min min min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 188 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S007 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:11:44 13C2-PFOA FC3G 013S007 100%- 7.14 2.57e5 994066 \ 0 - V i T-r min 7.00 8.00 13C8-PFOA FC3G 013S007 100- 7.14 8.15e4 315323 %- 07.00 tt min 8.00 13C4-PFOA FC3G_013S007 100 7.14 .17e5 444231 % 0 7.00 min 8.00 13C4-PFOS-1 FC3G_013S007 100 8.41 1.12e4 min 8.00 10.00 12.00 13C4-PFOS-2 FC3G_013S007 100- 8.41 1.01e4 41336 % 0 min 8.00 10.00 12.00 18O2-PFHxS-1 FC3G 013S007 18O2-PFHxS-2 FC3G 013S007 13C4-PFBA FC3G 013S007 13C2-PFHxA FC3G 013S007 13C5-PFNA FC3G 013S007 13C2-PFDA FC3G 013S007 13C2-PFDoA FC3G 013S007 min min min min min min min 13C8-PFOSA FC3G_013S007 100- 9.96 1.61e4 83206 %- 13C2-PFOUEA FC3G 013S007 0 min 8.00 10.00 12.00 min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 189 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S007 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:11:44 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218.... 5.99 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.51 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.41 23 498.93> 98.96 8.41 31 498 > 78 9.96 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.24 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.96 458.98 >393.98 7.43 Area 45779 67138 130876 146028 146755 33767 148161 190115 234886 286387 37888 20644 35921 19357 12138 31778 21613 28377 256975 81521 116856 11195 10080 13066 8344 23689 62914 83045 93912 174079 16141 30462 IS Area 23689 62914 62914 62914 256975 256975 83045 93912 93912 174079 13066 8344 13066 8344 13066 11195 10080 16141 116856 116856 116856 116856 116856 116856 116856 116856 116856 116856 116856 116856 ng/g 39.474 35.482 40.497 40.341 38.027 38.698 37.217 39.447 40.418 44.003 76.343 83.291 74.702 79.505 77.034 79.662 71.699 38.844 68.946 11.171 15.082 29.976 33.108 35.656 34.234 19.801 22.002 21.623 18.293 20.546 20.351 10.774 LOD 0.0261 0.2270 0.1114 0.0096 0.1470 0.2068 0.1372 0.1351 0.2271 0.5986 0.0550 0.0300 0.0624 0.1144 0.0503 0.0108 %Rec 98.7 88.7 101.2 100.9 95.1 96.7 93.0 98.6 101.0 110.0 95.4 104.1 93.4 99.4 96.3 99.6 89.6 97.1 95.8 93.1 125.7 83.3 92.0 99.0 95.1 82.5 91.7 90.1 76.2 85.6 84.8 107.7 S/N 460 1692 1704 2276 6798 0 1221 1408 4601 3927 9176 3296 766 0 0 355 745 1329 1889 3552 308 460 781 824 464 98 1397 2215 844 625 1646 3001 Noise 364 181 337 240 88 0 475 487 215 303 18 29 176 0 0 292 101 79 526 89 1443 98 53 63 70 907 199 146 406 1229 51 39 Height 167582 305855 574333 547200 597366 137348 580471 685265 989187 1189540 166672 94814 134378 71137 44761 103681 75105 105037 994066 315323 444231 44971 41336 51668 32517 88618 278315 322936 342479 767396 83206 117556 IS Height 88618 278315 278315 278315 994066 994066 322936 342479 342479 767396 51668 32517 51668 32517 51668 44971 41336 83206 444231 444231 444231 444231 444231 444231 444231 444231 444231 444231 444231 444231 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 190 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-E,,1/2 Name: FC3G_013S008 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:28:37 PFBA FC3G 013S008 PFPeA FC3G 013S008 PFHxA FC3G 013S008 PFHpA FC3G 013S008 PFOA-1 FC3G 013S008 PFOA-2 FC3G 013S008 min min min min min PFNA FC3G 013S008 100- 7.55 7.91e5 2958915 %- J 07.00 PFHxS-1 FC3G 013S008 PFDA FC3G_013S008 100 8.05 1.04e6 3722971 % PFUnA FC3G 013S008 min 8.00 0 min 8.00 10.00 12.00 PFHxS-2 FC3G 013S008 PFHxS-3 FC3G 013S008 100- 7.33 5.69e4 201711 %- PFDoA FC3G 013S008 min PFOS-1 FC3G 013S008 100- 8.35 1.65e5 481251 %- PFBS-1 FC3G 013S008 min PFOS-2 FC3G_013S008 100 8.41 1 .08e5 362848 % PFBS-2 FC3G 013S008 min PFOSA FC3G 013S008 min min 0 - t min 0 - V 9.96 min 0 min 7.00 8.00 8.00 10.00 12.00 8.00 10.00 12.00 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 191 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-E,,1/2 Name: FC3G_013S008 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:28:37 13C2-PFOA FC3G 013S008 100%- 7.14 2.20e5 851947 \ J V, 0 - T-r min 7.00 8.00 13C8-PFOA FC3G 013S008 100%- 7.14 7.29e4 286060 \ J 07.00 =rr min 8.00 13C4-PFOA FC3G_013S008 100 7.09 , 9.58e4 392720 % 0 7.00 min 8.00 13C4-PFOS-1 FC3G 013S008 18O2-PFHxS-2 FC3G 013S008 13C4-PFBA FC3G 013S008 13C2-PFHxA FC3G 013S008 13C5-PFNA FC3G 013S008 13C4-PFOS-2 FC3G 013S008 min 13C2-PFDA FC3G 013S008 18O2-PFHxS-1 FC3G 013S008 min 13C2-PFDoA FC3G 013S008 min min min min min min min 13C8-PFOSA FC3G 013S008 13C2-PFOUEA FC3G 013S008 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 192 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-E,,1/2 Name: FC3G_013S008 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:28:37 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.41 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.09 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.26 21 314.97>269.98 6.34 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.96 458.98 >393.98 7.43 Area 255608 336833 610449 638000 720547 163932 791016 1042945 1347697 1640496 178976 93640 174989 86452 56878 164663 108199 177335 219864 72926 95773 11328 9161 11125 6980 24224 55802 74331 96533 191720 18940 30315 IS Area 24224 55802 55802 55802 219864 219864 74331 96533 96533 191720 11125 6980 11125 6980 11125 11328 9161 18940 95773 95773 95773 95773 95773 95773 95773 95773 95773 95773 95773 95773 ng/g 220.277 200.881 214.713 199.318 219.330 219.931 222.788 212.560 226.688 231.852 424.077 455.894 427.668 426.879 426.669 410.612 399.187 208.283 71.975 12.193 12.361 37.010 36.713 37.040 34.945 24.706 23.811 23.614 22.943 27.609 29.138 10.722 LOD 4.1083 0.0341 0.0399 0.1185 0.0741 0.0076 0.0947 0.1511 0.2966 0.0206 0.1154 0.5904 0.0717 0.0236 0.0635 0.0819 0.0753 0.0491 %Rec 110.1 100.4 107.4 99.7 109.7 110.0 111.4 106.3 113.3 115.9 106.0 114.0 106.9 106.7 106.7 102.7 99.8 104.1 100.0 101.6 103.0 102.8 102.0 102.9 97.1 102.9 99.2 98.4 95.6 115.0 121.4 107.2 S/N 124 7323 6667 10287 41534 0 3532 6927 6793 11958 16927 4810 6713 0 0 1797 1585 5818 2754 4603 392 739 334 5201 823 115 1189 2591 946 1044 1366 656 Noise 7455 199 440 244 69 0 838 537 856 584 45 83 91 0 0 268 229 113 309 62 1003 63 101 8 32 791 230 102 365 777 67 169 Height 926163 1459708 2936564 2505653 2873672 652687 2958915 3722971 5811819 6980212 756561 399067 611636 288492 201711 481251 362848 655614 851947 286060 392720 46719 33762 44082 25941 90727 273066 263093 345133 811346 91462 110888 IS Height 90727 273066 273066 273066 851947 851947 263093 345133 345133 811346 44082 25941 44082 25941 44082 46719 33762 91462 392720 392720 392720 392720 392720 392720 392720 392720 392720 392720 392720 392720 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 bb 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 193 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-F,,1/2 Name: FC3G_013S009 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:45:38 PFBA FC3G 013S009 PFPeA FC3G 013S009 PFHxA FC3G 013S009 PFHpA FC3G 013S009 PFOA-1 FC3G 013S009 PFOA-2 FC3G 013S009 min min PFNA FC3G 013S009 100- 7.55 1.50e6 5454400 %- PFDA FC3G_013S009 100 8.05 2.12e6 '591859 % PFUnA FC3G 013S009 07.00 min 8.00 0 min 8.00 10.00 12.00 PFHxS-1 FC3G 013S009 100- 7.38 3.21e5 1086340 %- PFHxS-2 FC3G_013S009 100 7.38 _ 1.83e5 643061 % PFHxS-3 FC3G 013S009 07.00 min 8.00 0 7.00 min 8.00 min PFDoA FC3G 013S009 min PFOS-1 FC3G 013S009 min min PFBS-1 FC3G 013S009 min PFOS-2 FC3G 013S009 min min PFBS-2 FC3G 013S009 min min min PFOSA FC3G_013S009 100- 9.99 3.65e5 1349392 %- min 0 min 8.00 10.00 12.00 PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 194 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-F,,1/2 Name: FC3G_013S009 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:45:38 13C2-PFOA FC3G 013S009 100- 7.14 2.19e5 875102 %- J 07.00 =n min 8.00 13C8-PFOA FC3G 013S009 100- 7.14 7.14e4 286636 %- 07.00 T-r min 8.00 13C4-PFOA FC3G_013S009 100 7.14 9.27e4 362029 % 0 7.00 min 8.00 13C4-PFOS-1 FC3G 013S009 18O2-PFHxS-2 FC3G 013S009 13C4-PFBA FC3G 013S009 13C2-PFHxA FC3G 013S009 13C5-PFNA FC3G 013S009 13C4-PFOS-2 FC3G 013S009 min 13C2-PFDA FC3G 013S009 18O2-PFHxS-1 FC3G 013S009 min 13C2-PFDoA FC3G 013S009 min min min min min min min 13C8-PFOSA FC3G 013S009 13C2-PFOUEA FC3G 013S009 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 195 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-F,,1/2 Name: FC3G_013S009 Description: 1,,15uL Date: 11-Jan-2013 Time: 14:45:38 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.24 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.78 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.64 30 612.95 > 568.... 9.27 24 298.94> 79.96 6.51 25 298.94 > 98.95 6.51 24 398.84 > 79.96 7.38 25 398.84> 98.96 7.38 24 398.84> 118.70 7.38 22 498.93> 79.96 8.41 23 498.93> 98.96 8.41 31 498 > 78 9.99 21 414.97 > 369.... 7.14 21 420.97 > 375.... 7.14 416.97 > 371.9 7.14 21 503 > 79.96 8.41 21 503 > 98.96 8.41 21 402.9>84 7.38 21 402.9>103 7.38 21 216.99>171.98 5.26 21 314.97>269.98 6.36 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.99 458.98 >393.98 7.43 Area 529256 733203 1180792 1404129 1356679 315010 1497967 2117893 2783679 3481032 333040 176912 321443 183159 102356 339460 241234 364640 218928 71423 92721 12244 9863 9932 7888 26173 52550 81811 102880 214405 19590 30111 IS Area 26173 52550 52550 52550 218928 218928 81811 102880 102880 214405 9932 7888 9932 7888 9932 12244 9863 19590 92721 92721 92721 92721 92721 92721 92721 92721 92721 92721 92721 92721 ng/g 423.109 464.376 441.448 466.012 414.938 424.491 383.440 405.438 439.560 440.561 884.007 762.814 879.991 800.785 860.628 783.790 827.661 414.386 74.028 12.334 11.967 41.318 40.828 34.157 40.790 27.572 23.162 26.846 25.256 31.892 31.130 10.650 LOD 0.0796 0.0587 0.7288 0.0721 0.0095 0.0278 0.1918 0.1934 0.0762 0.0530 0.3357 0.0462 0.0367 0.0757 0.0925 0.0647 0.0119 %Rec 105.8 116.1 110.4 116.5 103.7 106.1 95.9 101.4 109.9 110.1 110.5 95.4 110.0 100.1 107.6 98.0 103.5 103.6 102.8 102.8 99.7 114.8 113.4 94.9 113.3 114.9 96.5 111.9 105.2 132.9 129.7 106.5 S/N 1863 10162 20565 25207 25056 0 5263 8285 21784 4243 47040 15977 3256 0 0 4253 2892 3875 3151 4024 1290 654 628 1211 2137 230 1905 2045 932 1103 1835 2674 Noise 983 308 269 209 225 0 1036 916 519 3422 29 44 334 0 0 236 289 348 278 71 281 74 61 29 13 415 136 146 402 809 53 45 Height IS Height 1830687 95441 3127445 259723 5537483 259723 5256633 259723 5647306 875102 1287049 875102 5454400 297518 7591859 374167 11302019 374167 14521640 892691 1386435 34943 697209 28524 1086340 34943 643061 28524 326226 34943 1004414 48411 835254 38196 1349392 97227 875102 362029 286636 362029 362029 48411 362029 38196 362029 34943 362029 28524 362029 95441 362029 259723 362029 297518 362029 374167 362029 892691 362029 97227 362029 121600 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 196 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-G,,1/2 Name: FC3G_013S010 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:02:39 PFBA FC3G 013S010 PFPeA FC3G 013S010 PFHxA FC3G 013S010 PFHpA FC3G 013S010 PFOA-1 FC3G 013S010 PFOA-2 FC3G 013S010 min min min min min PFNA FC3G 013S010 PFDA FC3G 013S010 PFUnA FC3G 013S010 PFDoA FC3G_013S010 100- 9.24 8.08e6 31703496 %- PFBS-1 FC3G 013S010 PFBS-2 FC3G 013S010 min min 0 min min 8.00 10.00 12.00 PFHxS-1 FC3G 013S010 A100%- \ 07.00 min 8.00 PFHxS-2 FC3G 013S010 100- 7.33 3.46e5 1088404 %- 07.00 min 8.00 PFHxS-3 FC3G 013S010 100- 7.33 2.08e5 677773 %- \ / 07.00 V+. t min 8.00 PFOS-1 FC3G 013S010 100- 8.35 7.45e5 1936700 1% - 08.00 9.99 10.00 min 12.00 PFOS-2 FC3G_013S010 100 8.35 5.03e5 1540466 % 0 min 8.00 10.00 12.00 PFOSA FC3G 013S010 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 197 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-G,,1/2 Name: FC3G_013S010 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:02:39 13C2-PFOA FC3G 013S010 100- 7.09 1.78e5 701478 \% - J V j0 - -TT min 7.00 8.00 13C8-PFOA FC3G 013S010 100- 7.09 5.80e4 235720 %- 07.00 -TT min 8.00 13C4-PFOA FC3G_013S010 100 7.09 , 7.63e4 309066 % 0 7.00 min 8.00 13C4-PFOS-1 FC3G 013S010 18O2-PFHxS-2 FC3G 013S010 13C4-PFBA FC3G 013S010 13C2-PFHxA FC3G 013S010 13C5-PFNA FC3G 013S010 13C4-PFOS-2 FC3G 013S010 min 13C2-PFDA FC3G 013S010 18O2-PFHxS-1 FC3G 013S010 min 13C2-PFDoA FC3G 013S010 min min min min min min min 13C8-PFOSA FC3G_013S010 100- 9.99 _ 1.98e4 94630 %- 13C2-PFOUEA FC3G 013S010 0 min 8.00 10.00 12.00 min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 198 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-G,,1/2 Name: FC3G_013S010 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:02:39 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.21 27 262.98 > 218.... 5.95 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.14 19 412.97> 168.80 7.14 28 462.96 > 418.... 7.55 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.49 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.23 21 314.97>269.98 6.34 21 467.96>422.97 7.55 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.24 21 506>78 9.99 458.98 >393.98 7.43 Area 1106351 1451118 2301871 2756213 2785893 654423 3206580 4358040 6297988 8082436 616948 325842 630934 345834 208209 744593 502724 935573 178447 57989 76343 9275 8107 7971 5514 21823 43564 64985 98693 246180 19776 26510 IS Area 21823 43564 43564 43564 178447 178447 64985 98693 98693 246180 7971 5514 7971 5514 7971 9275 8107 19776 76343 76343 76343 76343 76343 76343 76343 76343 76343 76343 76343 76343 ng/g 1062.375 1108.718 1038.652 1103.663 1045.711 1082.038 1033.584 870.216 1037.007 891.613 2040.651 2011.315 2152.301 2163.792 2182.301 2270.578 2099.973 1053.749 73.284 12.163 9.853 38.017 40.756 33.293 34.633 27.921 23.320 25.900 29.426 44.474 38.165 9.376 LOD 0.0056 0.0375 0.0797 1.0195 0.0788 0.2471 0.4177 0.1015 0.0101 0.0962 0.1915 0.2888 0.1262 0.0546 0.5500 0.1083 0.0517 0.0657 0.0939 0.0603 0.0734 %Rec 106.2 110.9 103.9 110.4 104.6 108.2 103.4 87.0 103.7 89.2 102.0 100.6 107.6 108.2 109.1 113.5 105.0 105.4 101.8 101.4 82.1 105.6 113.2 92.5 96.2 116.3 97.2 107.9 122.6 185.3 159.0 93.8 S/N 18584 63240 39460 73556 11748 0 13098 1730 15999 37235 35111 36917 19148 0 77975 4771 5738 9816 2103 3619 307 510 378 689 1550 129 770 1284 1190 1399 2244 383 Noise 204 93 280 140 947 0 857 8898 1581 851 67 32 102 0 9 406 268 336 334 65 1006 63 78 41 12 580 273 175 297 701 42 269 Height IS Height 3791494 74558 5903201 210376 11029682 210376 10302533 210376 11127175 701478 2625699 701478 11230852 224568 15396270 353488 25290048 353488 31703496 980671 2341614 28081 1197156 18186 1959121 28081 1088404 18186 677773 28081 1936700 32186 1540466 29278 3299352 94630 701478 309066 235720 309066 309066 32186 309066 29278 309066 28081 309066 18186 309066 74558 309066 210376 309066 224568 309066 353488 309066 980671 309066 94630 309066 103032 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MMX 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 199 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-H,,1/2 Name: FC3G_013S011 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:19:32 PFBA FC3G 013S011 PFPeA FC3G 013S011 PFHxA FC3G 013S011 PFHpA FC3G 013S011 PFOA-1 FC3G 013S011 PFOA-2 FC3G 013S011 PFNA FC3G 013S011 PFHxS-1 FC3G 013S011 min PFDA FC3G 013S011 min PFHxS-2 FC3G 013S011 min min PFUnA FC3G 013S011 min PFHxS-3 FC3G 013S011 min min min min PFDoA FC3G_013S011 100- 9.24 1.40e7 49788828 %- PFBS-1 FC3G 013S011 PFBS-2 FC3G 013S011 min 0 min 8.00 10.00 12.00 min PFOS-1 FC3G 013S011 100- 8.35 r 1.07e6 2454084 %- PFOS-2 FC3G_013S011 100 8.35 7.49e5 2091331 % PFOSA FC3G 013S011 Vi 9.99 min 0 - 8.00 10.00 12.00 0 min 8.00 10.00 12.00 min min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 200 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-H,,1/2 Name: FC3G_013S011 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:19:32 13C2-PFOA FC3G 013S011 100- 7.09 1.33e5 549876 \% - J V0- 7.00 - n min 8.00 13C8-PFOA FC3G 013S011 100- 7.09 4.13e4 165812 \% - 07.00 =n min 8.00 13C4-PFOA FC3G_013S011 100 7.09 5.42e4 1220898 % 0 7.00 min 8.00 13C4-PFOS-1 FC3G 013S011 18O2-PFHxS-2 FC3G 013S011 13C4-PFBA FC3G 013S011 13C2-PFHxA FC3G 013S011 13C5-PFNA FC3G 013S011 13C4-PFOS-2 FC3G 013S011 min 13C2-PFDA FC3G 013S011 18O2-PFHxS-1 FC3G 013S011 min 13C2-PFDoA FC3G 013S011 min min min min min min min 13C8-PFOSA FC3G 013S011 13C2-PFOUEA FC3G 013S011 min min PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 201 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\Inst_G\PFC.PRO\FC\FC3G_013-A1.qld Last Altered: Monday, January 14, 2013 13:08:53 Pacific Standard Time Printed: Monday, January 14, 2013 13:44:42 Pacific Standard Time ID: FC-LIN-42151T-H,,1/2 Name: FC3G_013S011 Description: 1,,15uL Date: 11-Jan-2013 Time: 15:19:32 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 18O2-PFHxS-1 25 18O2-PFHxS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.26 27 262.98 > 218.... 5.97 27 312.97> 268.98 6.34 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 > 418.... 7.49 29 512.96 > 468.... 8.05 29 562.96 > 518.... 8.61 30 612.95 > 568.... 9.24 24 298.94> 79.96 6.47 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.41 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9>103 7.33 21 216.99>171.98 5.24 21 314.97>269.98 6.33 21 467.96>422.97 7.49 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.21 21 506>78 9.99 458.98 >393.98 7.38 Area IS Area 1451154 13033 2412291 33387 3564080 33387 4155998 33387 4425141 132546 1008404 132546 4992039 44026 6485407 85123 9320023 85123 14006239 246757 910658 5135 464895 3683 875468 5135 486835 3683 289888 5135 1072351 6391 748686 5168 1753377 15345 132546 54200 41290 54200 54200 6391 54200 5168 54200 5135 54200 3683 54200 13033 54200 33387 54200 44026 54200 85123 54200 246757 54200 15345 54200 19906 ng/g 2334.523 2404.932 2098.793 2171.579 2236.502 2244.799 2375.365 1501.794 1779.414 1542.003 4675.857 4298.276 4635.939 4561.884 4717.185 4746.605 4907.534 2545.530 76.671 12.198 6.996 36.896 36.593 30.210 32.577 23.488 25.174 24.714 35.749 62.790 41.713 7.040 LOD 0.3175 0.1590 0.4566 0.0846 0.0536 0.4240 2.6270 3.0748 2.8822 0.1097 0.0294 0.0292 0.2022 0.2100 0.6443 0.1409 1.0759 0.0599 0.0437 0.1550 0.0834 0.1188 0.0522 %Rec 93.4 96.2 84.0 86.9 89.5 89.8 95.0 60.1 71.2 61.7 93.5 86.0 92.7 91.2 94.3 94.9 98.2 101.8 106.5 101.7 58.3 102.5 101.6 83.9 90.5 97.9 104.9 103.0 149.0 261.6 173.8 70.4 S/N 4954 30997 45309 34263 9648 0 29400 10240 19203 81743 31464 27854 4897 0 0 2634 3303 24758 2134 1227 718 446 418 111 525 56 1475 1468 597 2038 1157 405 Noise 995 315 383 450 1889 0 605 2228 1777 609 111 60 542 0 0 932 633 230 258 135 308 48 40 149 22 811 108 106 501 446 59 182 Height IS Height 4931902 45394 9763751 159099 17371712 159099 15421288 159099 18224222 549876 4135016 549876 17796560 155374 22815656 299509 34117992 299509 49788828 907986 3500984 16511 1658313 11370 2653335 16511 1450628 11370 863093 16511 2454084 21217 2091331 16834 5702633 68691 549876 220898 165812 220898 220898 21217 220898 16834 220898 16511 220898 11370 220898 45394 220898 159099 220898 155374 220898 299509 220898 907986 220898 68691 220898 73576 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factor2 Detection... 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MM 1.0 MMX 1.0 MMX 1.0 MM PV by LIU 14-Jan-13, SV by AP 14-Jan-13. NOTE: Std. Concn. & LOD units = ng abs. Excluded points denoted with an 'X' in the Detection Flag column. Page 202 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time Method: G:\Masslynx\Master Methods\PFC\FC_043_08_02.mdb 06 Nov 2012 13:32:38 Calibration: G:\Masslynx\lnst_G\PFC.PRO\CurveDB\FC3G_013-A1.cdb 14 Jan 2013 13:08:53 ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S014 Description: 1,,15uL Date: 11-Jan-2013 Time: 16:10:30 PFBA FC3G013S014 100-, 5.23 4.10e4 155020 5.00 6.00 7.00 PFNA FC3G_013S014 100-, 7.49 1.41e5~| 533591 f t %- PFPeA FC3G013S014 100- 5.97 6.42e4 i| 287375 i: M %" 5.00 6.00 7.00 PFDA FC3G_013S014 100- _ 8.05 1.90e5 700937 %- PFHxA FC3G_013S014 100- 6.36 1.24e5 555146 % 1 x M i W " min 5.00 6.00 7.00 PFHpA FC3G_013S014 100 6.73 1.32e5 \ 519650 h %- 5.00 6.00 7.00 PFUnA FC3G_013S014 100 8.58 2.30e5 I 998229 PFDoA FC3G 013S014 100- 9.21 2.57e5 1071677 % 7.00 8.00 PFHxS-1 FC3G_013S014 100- 7.33 3.33e4 \ 126745 %- 7.00 8.00 8.00 10.00 12.00 PFHxS-2 FC3G_013S014 III100'-- |1 7.33 .94e 4 "l ` 74496 %- 7.00 8.00 8.00 10.00 12.00 PFHxS-3 FC3G 013S014 100- 7.33 1.14e4 A 41849 . \ %- 7.00 8.00 8.00 10.00 12.00 PFOS-1 FC3G_013S014 1f|f| 8.35 n I" 3.05e4 1 89546 %- 0-+i< 8.00 9.99 10.00 12.00 PFOA-1 FC3G_013S014 100- 7.09 ; 1.37e5 52072? %- 7.00 8.00 PFBS-1 FC3G_013S014 100- 6.49 3.48e4 "1 1495677 Ii %- jl 5.00 6.00 7.00 PFOS-2 FC3G_013S014 100- __ 8.35 2.05e4 69582 %- i" 8.00 10.00 12.00 PFOA-2 FC3G 013S014 PFBS-2 FC3GJ513S014 100- 6.47 _ 1.84e4 81465 %- 5.00 6.00 7.00 PFOSA FC3G_013S014 100- 9.99 2.92e4 110473 I %- _ 8.00 10.00 12.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. Page 203 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S014 Description: 1,,15uL Date: 11-Jan-2013 Time: 16:10:30 13C2-PFOA FC3G_013S014 100- 7.09 2.33e5 908268 %- o 13C8-PFOA FC3G_013S014 7.09 7.87e4 . .303582 %- 13C4-PFOA FC3GJM3S014 100- 7.09 T 9.38e4 366684 %- 0 - t+ . - ,. .n.p-.---I min 7.00 8.00 uo -^ ^ ; ';T_r r T T v r.v i i M-r-ri min 7.00 8.00 i u0 ^- , . . ..........................-- min 7.00 8.00 1802-PFHxS-2 FC3G_013S014 100^--7, 7.33 .47e _ 3" l 29494 A %- 7.00 8.00 13C4-PFBA FC3G_013S014 100- 5.24 f 2.22e4 83612 %4.28 I --H.5.00 6.00 7.00 13C2-PFHxA FC3G_013S014 100- 6.36 5.93e4" l 265916 %- ntrrpT _l 5.00 6.00 7.00 13C8-PFOSA FC3G_013S014 100- 9.99 1.60e4~j 80297 S %- 13C2-PFOUEA FC3G_013S014 100- 3 7.38 .13e 4 ~ l ` 119413 a %- 8.00 10.00 12.00 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S014 100n 8.35 f 1.06e4 1 40785 %-- li 1 .I!'.%-wrp ' li 1 1 11 1 , .vi-- mminn 8.00 10.00 12.00 OoJ 1 ' 1 13C4-PFOS-2 FC3G 013S014 8.35 9.19e3 ! 35760 %: j ,p 8.00 10.00 12.00 13C5-PFNA FC3G 013S014 13C2-PFDA FC3G_013S014 100 8.05 9.02e4 317323 1802-PFHxS-l FC3G_013S014 100 ^ 7.33 1 .0 7 e 4 l 42422 11 %: 7.00 8.00 1302-PFDoA FC3G_013S014 100- 9.21 1.53e5 645665 8.00 10.00 12.00 ""lN r 8.00 10.00 12.00 m Page 204 of 234 vww.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3GJJ13S014 Description: 1,,15uL Date: 11-Jan-2013 Time: 16:10:30 w ~ Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-l 25 1802-PFHXS-2 26 13C4-PFBA 27 13C2-PFHxA 28 13C5-PFNA 29 13C2-PFDA 30 1302-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA is# T race KT 26 212.99>168.98 5.23 27 262.98 >218.... 5.97 27 312.97> 268.98 6.36 27 362.97> 318.98 6.73 19 412.97> 368.98 7.09 19 412.97 168.80 7.09 28 462.96 >418.... 7.49 29 512.96 >468.... 8.05 29 562.96 >518.... 8.58 30 612.95 > 568.... 9.21 24 298.94> 79.96 6.49 25 298.94 > 98.95 6.47 24 398.84 > 79.96 7.33 25 398.84> 98.96 7.33 24 398.84> 118.70 7.33 22 498.93> 79.96 8.35 23 498.93> 98.96 8.35 31 498 > 78 9.99 21 414.97 > 369.... 7.09 21 420.97 > 375.... 7.09 416.97 > 371.9 7.09 21 503 > 79.96 8.35 21 503 > 98.96 8.35 21 402.9>84 7.33 21 402.9103 7.33 21 216.99>171.98 5.24 21 314.97>269.98 6.36 21 467.96>422.97 7.49 21 514.96 >469.96 8.05 21 614.95 > 569.... 9.21 21 506>78 9.99 458.98 >393.98 7.38 Area 40975 64184 123830 132304 137068 33833 140845 190499 229964 257323 34760 18417 33292 19432 11407 30529 20510 29207 233423 78698 93820 10614 9188 10732 7467 22226 59287 79295 90207 152994 15953 31291 IS Area 22226 59287 59287 59287 233423 233423 79295 90207 90207 152994 10732 7467 10732 7467 10732 10614 9188 15953 93820 93820 93820 93820 93820 93820 93820 93820 93820 93820 93820 93820 ng/fi 37.609 35.996 40.663 38.780 39.107 42.693 37.052 41.170 41.201 45.002 85.290 83.028 84.302 89.255 88.235 80.728 74.688 40.467 78.004 13.432 12.109 35.398 37.586 36.475 38.158 23.140 25.824 25.715 21.886 22.491 25.052 11.067 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. LOD 0.0568 0.2160 0.0662 0.0241 0.0505 0.0977 0.2038 0.1160 0.2353 0.0969 0.6682 0.0312 0.0189 0.2417 0.1008 0.0827 0.0428 %Rec 94.0 90.0 101.7 96.9 97.8 106.7 92.6 102.9 103.0 112.5 106.6 103.8 105.4 111.6 110.3 100.9 93.4 101.2 108.3 111.9 100.9 98.3 104.4 101.3 106.0 96.4 107.6 107.1 91.2 93.7 104.4 110.7 S/N 200 1130 1505 1956 8223 441 3613 1970 2104 2106 5491 1886 2096 0 0 851 488 1634 9649 788 372 512 968 470 1194 100 2847 3924 245 723 1171 776 Noise 776 254 369 266 63 298 148 356 474 509 27 43 60 0 0 105 142 68 94 385 986 80 37 90 25 836 93 76 1298 893 69 154 Height 155020 287375 555146 519650 520722 131702 533591 700937 998229 1071677 149567 81465 126745 74496 41849 89546 69582 110473 908268 303582 366684 40785 35760 42422 29494 83612 265916 298638 317323 645665 80297 119413 IS Height 83612 265916 265916 265916 908268 908268 298638 317323 317323 645665 42422 29494 42422 29494 42422 40785 35760 80297 366684 366684 366684 366684 366684 366684 366684 366684 366684 366684 366684 366684 Sample size 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 Factors Detection... 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 0 Page 205 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\l nst.G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3GJH3S038 Description: 1,,15uL Date: H^Jan-2013 Time: 22:57:42 PFBA FC3G 013S038 100- 5.19 1 4.68e4 i 174507 %- PFPeA FC3G 013S038 5.95 100- 6.90e41 308459 PFHxA FC3G 013S038 100- 6.33 1.37e5 1 631423 %: 0 - Lttt - ^T ^rr'tt min 5.00 6.00 7.00 0 -tn r rr......1!1n111^ 1" i1-rr min 5.00 6.00 7.00 0- i , 5.00 6.00 7.00 PFNA FC3G_013S038 ~l AC 100 1 1.47e5 608993 %- L. 7.00 - . =" min 8.00 PFDA FC3G 013S038 o S~ 1.97e5 1 714684 %fj 0" J \ 8.00 min 10.00 12.00 PFUnA FC3G__013S038 100- 8.55 f 2.50e5 - I 1060816 %: 0-* 8.00 10.00 12.00 PFHxS-1 FC3G 013S038 1001 7.29 /T 3.19e4 119600 %- o- -------------- :-------p --- min 7.00 8.00 PFHxS-2 FC3G 013S038 100- 7.29 ' 1.73e4 62998 Ifilt u0--^4I -.r p --r r Tr-p rrr-ri i iri4 mminin 7.00 8.00 PFHxS-3 FC3G 013S038 100- - 7.29 1.02e4 \ 35856 %7 u0--- rr ----------------- " --1-1 min 7.00 8.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. PFHpA FC3G_013S038 100- 6.68 1.38e5 548581 I %- TjnTf , ri . 111-iTj 11iVi jfr 5.00 6.00 7.00 PFDoA FC3G_013S038 100-, 9.15 2.44e5 976177 %- PFOA-1 FC3G_013S038 m n 709 100 \ 1.44e5 m 549281 %" --I 7.00 I.. 8.00 PFBS-1 FC3G 013S038 8.00 10.00 12.00 PFOS-1 FC3G 013S038 PFOS-2 FC3G_013S038 100- 8.29 T 1,98e4 I 66270 %- oO! PFOA-2 FC3G_013S038 7.09 r . 3.57e4 ... W\ 139673 %: m 07.00 8.00 PFBS-2 FC3G_013S038 6.44 100- 1.85e4 84958 %- -TJT1 5.00 6.00 7.00 PFOSA FC3G_013S038 100 9.96 2.70e4 I 97741 1 %- 8.00 10.00 12.00 8.00 10.00 12.00 m Page 206 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21, 2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31, 2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S038 Description: 1,,15uL Date: 11-Jan-2013 Time: 22:57:42 13C2-PFOA FC3G_013S038 100n - 2.47e5 952397 - 13C8-PFOA FC3G 013S038 100- - f 8.34e4 .323247 %- 13C4-PFOA FC3G_013S038 100^ . 7.05 | 1.07e5 1416902 %: 7.00 _ m min 8.00 1802-PFHxS-2 FC3G013S038 100 7.29 '7 .2 3 0 3 ,28847 %- 07.00 13C4-PFBA FC3G 013S038 r-T-r min 8.00 o: 7.00 8.00 13C2-PFHxA FC3G013S038 100- 6.33 6.51e4 J 299879 7.00 8.00 13C8-PFOSA FC3G_013S038 100 9.99 1.44e4~] 71975 I %- 13C2-PFOUEA FC3G_013S038 0- min 8.00 10.00 12.00 5.00 6.00 7.00 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. 13C4-PFOS-1 FC3G 013S038 io o n 8.29 I 1,06e4 I 40433 %I ft 0- L 8.00 ....... min 10.00 12.00 13C4-PFOS-2 FC3G 013S038 100- 8.29 i " 8.59e3 l 31283 %J (H min 8.00 10.00 12.00 1802-PFHxS-1 FC3G_013S038 100-, 7.29 |"l.0 7 e 4 40670 0-* 7.00 8.00 13C5-PFNA FC3G 013S038 13C2-PFDA FC3G013S038 7 99 100^ f 9 5 4 ' 340457 13C2-PFDoA FC3G 013S038 o4 8.00 10.00 12.00 m Page 207 of 234 www.axysanalytical.com Quantify Sample Report MassLynx 4.1 SCN627 Dataset: G:\Masslynx\lnst_G\PFC.PRO\FC\FC3G_013-B.qld Last Altered: Monday, January 21,2013 14:06:06 Pacific Standard Time Printed: Thursday, January 31,2013 11:46:23 Pacific Standard Time ID: FC-LIN-42151T-D,,1/2 Name: FC3G_013S038 Description: 1,,15uL Date: 11-Jan-2013 Time: 22:57:42 # Name 1 PFBA 2 PFPeA 3 PFHxA 4 PFHpA 5 PFOA-1 6 PFOA-2 7 PFNA 8 PFDA 9 PFUnA 10 PFDoA 11 PFBS-1 12 PFBS-2 13 PFHxS-1 14 PFHxS-2 15 PFHxS-3 16 PFOS-1 17 PFOS-2 18 PFOSA 19 13C2-PFOA 20 13C8-PFOA 21 13C4-PFOA 22 13C4-PFOS-1 23 13C4-PFOS-2 24 1802-PFHxS-1 25 1802-PFHxS-2 26 13C4-PFBA 27 13C2-PFHXA 28 13C5-PFNA 29 13C2-PFDA 30 13C2-PFDoA 31 13C8-PFOSA 32 13C2-PFOUEA IS# Trace RT 26 212.99>168.98 5.19 27 262.98 >218.... 5.95 27 312.97> 268.98 6.33 27 362.97> 318.98 6.68 19 412.97> 368.98 7.09 19 412.97> 168.80 7.09 28 462.96 >418.... 7.46 29 512.96 >468.... 7.99 29 562.96 > 518.... 8.55 30 612.95 > 568.... 9.15 24 298.94> 79.96 6.47 25 298.94 >98 .95 6.44 24 398.84 > 79.96 7.29 25 398.84> 98.96 7.29 24 398.84> 118.70 7.29 22 498.93> 79.96 8.29 23 498.93> 98.96 8.29 31 498 > 78 9.96 21 414.97 > 369.... 7.05 21 420.97 > 375.... 7.05 416.97 >371.9 7.05 21 503 > 79.96 8.29 21 503 > 98.96 8.29 21 402.9>84 7.29 21 402.9>103 7.29 21 216.99>171.98 5.19 21 314.97>269.98 6.33 21 467.96>422.97 7.46 21 514.96 >469.96 7.99 21 614.95 > 569.... 9.15 21 506>78 9.99 458.98 >393.98 7.33 Area 46783 69047 136739 137847 143677 35654 146649 196747 249575 243755 35074 18515 31945 17318 10159 28693 19846 26969 246572 83449 107314 10647 8588 10708 7226 25606 65085 81013 95783 143341 14423 35723 IS Area 25606 65085 65085 65085 246572 246572 81013 95783 95783 143341 10708 7226 10708 7226 10708 10647 8588 14423 107314 107314 107314 107314 107314 107314 107314 107314 107314 107314 107314 107314 ng/g 37.26' 35.274 40.904 36.798 38.805 42.592 37.764 40.032 42.117 45.507 86.254 86.292 81.072 82.148 78.688 75.597 77.346 41.334 72.037 12.452 13.851 31.043 30.716 31,818 32.285 23.307 24.785 22.969 20.316 18.422 19.803 12.635 LOD 0.0255 0.0044 0.0019 0.0131 0.0103 0.0499 0.0975 0.3189 0.3437 0.1200 0.2752 0.0483 0.0477 0.0603 0.0804 0.0774 0.0489 PV by LHE, SV by KJC 31-Jan-13. Note: Calver = ng abs. SPM = ng/mL. %Rec S/N 93.2 615 88.2 1598 102.3 2186 92.0 4171 97.0 1385 106.5 1639 94.4 5614 100.1 2423 105.3 3897 113.8 3291 107.8 3943 107.9 6867 101.3 4613 102.7 0 98.4 461 94.5 1836 96.7 749 103.3 2207 100.1 16363 103.8 3623 115.4 834 86.2 934 85.3 271 88.4 272 89.7 829 97.1 230 103.3 1826 95.7 1546 84.7 76.8 925 722 82.5 986 126.3 774 Noise 284 193 289 132 397 85 108 295 272 297 39 12 26 0 78 45 89 44 58 89 500 43 115 150 35 392 164 218 368 810 73 178 Height IS Height Sample size 174507 90175 1.0000 308459 299879 1.0000 631423 299879 1.0000 548581 299879 1.0000 549281 952397 1.0000 139673 952397 1.0000 608993 337008 1.0000 714684 340457 1.0000 1060816 340457 1.0000 976177 584559 1.0000 153073 40670 1.0000 84958 28847 1.0000 119600 40670 1.0000 62998 28847 1.0000 35856 40670 1.0000 82662 40433 1.0000 66270 31283 1.0000 97741 71975 1.0000 952397 416902 1.0000 323247 416902 1.0000 416902 1.0000 40433 416902 1.0000 31283 416902 1.0000 40670 416902 1.0000 28847 416902 1.0000 90175 416902 1.0000 299879 416902 1.0000 337008 416902 1.0000 340457 416902 1.0000 584559 416902 1.0000 71975 416902 1.0000 137578 1.0000 Factor2 Detection.. 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 MM 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb 1.0 bb Page 208 of 234 www.axysanalytical.com Matrix Codes for Table 1a NPW = Non-Potable Water DrW = Drinking Water S = Solid T = Tissue AXYS Analytical Services Ltd. Table 1a NELAP Accreditation Held by AXYS Analytical Services Ltd. for Chlorinated Dioxins/Furans, Chlorinated Pesticides, PCBs and PAHs Accreditation Method Codes and Explanation for Table 1a Code Accreditation Certificate Applicable AXYS Method and Description No. Method Reference 1 EPA 1613B MLA-017, performance based implementation of EPA1613B (GC/HRMS) 2 EPA 8290 MLA-017, performance based implementation of EPA 8290 (Gc /HRMS) 3 AXYS MLA-017 MLA-017, performance based implementation of EPA 1613B, 8290 (GC/HRMS) 4 EPA 608 MLA-007, performance based implementation of EPA 608 (GC/ECD) 5 EPA 8270C or 8270D MLA-007, performance based modification of 8270C/D (GC/LRMS) 6 E P A 8081A or8081B MLA-007, performance based implementation of EPA 8 0 8 1 A/B (GC/ECD) 7 EPA 1668A MLA-010, performance based implementation of EPA 1668A (GC/HRMS) 8 SM 6630B MLA-007, performance based implementation of SM 18-20 6630B (GC/ECD) 9 EPA 1625B MLA-021, performance based modification of EPA 1625B (GC/LRMS) 11 EPA 625 MLA-007, performance based modification of EPA 625 (g C/LRMS) 20 EPA 8270C or 8270D MLA-021, performance based modification of EPA 8270C/D (GC/LRMS) 21 EPA 1625B MLA-007, performance based modification of EPA 1625B (GC/LRMS) ACC-101 Rev 08, 08-Nov-2012 P ^ e ro J l Page 209 of 234 www.axysanalytical.com TABLE 1a PCDD/F - Polychlorinated Dioxins and Furans Dioxins Dioxins and Dibenzofurans 1,2,3,4,6,7,8-HpCDD 1,2,3,4,6,7,8-HpCDF 1,2,3,4,7,8,9-HpCDF 1,2,3,4,7,8-HxCDD 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDD 1,2,3,6,7,8-HxCDF 1,2,3,7,8,9-HxCDD 1,2,3,7,8,9-HxCDF 1,2,3,7,8-PeCDD 1,2,3,7,8-PeCDF 2,3,4,6,7,8-HxCDF 2,3,4,7,8-PeCDF 2,3,7,8-TCDD 2,3,7,8-TCDF OCDD OCDF Total TCDD Total TCDF Total PeCDD Total PeCDF Total HxCDD Total HxCDF Total HpCDD Total HpCDF PCBs - Polychlorinated biphenyls PCB 1 2-Chlorobiphenyl PCB 3 4-Chlorobiphenyl PCB 4 2,2'-Dichlorobiphenyl PCB 5 2,3-Dichlorobiphenyl PCB 15 4,4'-Dichlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. New York California State Department Department of Public of Health Health Lab ID 11674 Lab ID 01138CA NELAP NELAP P rim a ry Secondary NP W S NP W S State of Florida Department of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 1 2 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 1 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 12 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 1 1, 2, 3 2, 3 2, 3 State of New Jersey Department of Environmental Protection Lab ID C AN A005 NELAP Secondary Dr. NP WW S T 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 122 22 22 22 22 22 22 22 22 Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP W S T 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 1, 2 2 22 22 22 22 22 22 22 22 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 P39e2oJ l Page 210 of 234 www.axysanalytical.com TABLE 1a PCB 18 PCB 19 PCB 31 PCB 37 PCB 44 PCB 52 PCB 54 PCB 66 PCB 77 PCB 81 PCB 87 PCB 101 PCB 104 PCB 105 PCB 109 PCB 114 PCB 118 PCB 123 PCB 124 PCB 126 PCB 138 PCB 141 PCB 151 PCB 153 PCB 155 PCB 156 PCB 157 PCB 167 PCB 169 PCB 170 PCB 180 PCB 183 PCB 187 PCB 188 PCB 189 PCB 202 2,2',5-T richlorobiphenyl 2,2',6-T richlorobiphenyl 2,4',5-Trichlorobiphenyl 3,4,4'-Trichlorobiphenyl 2,2',3,5'-Tetrachlorobiphenyl 2,2',5,5'-Tetrachlorobiphenyl 2,2',6,6'-Tetrachlorobiphenyl 2,3',4,4'-Tetrachlorobiphenyl 3,3',4,4'-Tetrachlorobiphenyl 3,4,4',5-Tetrachlorobiphenyl 2,2',3,4,5'-Pentachlorobiphenyl 2,2',4,5,5'-Pentachlorobiphenyl 2,2',4,6,6'-Pentachlorobiphenyl 2,3,3',4,4'-Pentachlorobiphenyl 2,3,3',4,6-Pentachlorobiphenyl 2,3,4,4',5-Pentachlorobiphenyl 2,3',4,4',5-Pentachlorobiphenyl 2,3',4,4',5'-Pentachlorobiphenyl 2,3',4',5,5'-Pentachlorobiphenyl 3,3',4,4',5-Pentachlorobiphenyl 2,2',3,4,4',5'-Hexachlorobiphenyl 2,2',3,4,5,5'-Hexachlorobiphenyl 2,2',3,5,5',6-Hexachlorobiphenyl 2,2',4,4',5,5'-Hexachlorobiphenyl 2,2',4,4',6,6'-Hexachlorobiphenyl 2,3,3',4,4',5-Hexachlorobiphenyl 2,3,3',4,4',5'-Hexachlorobiphenyl 2,3',4,4',5,5'-Hexachlorobiphenyl 3,3',4,4',5,5'-Hexachlorobiphenyl 2,2',3,3',4,4',5-Heptachlorobiphenyl 2,2',3,4,4',5,5'-Heptachlorobiphenyl 2,2',3,4,4',5',6-Heptachlorobiphenyl 2,2',3,4',5,5',6-Heptachlorobiphenyl 2,2',3,4',5,6,6'-Heptachlorobiphenyl 2,3,3',4,4',5,5'-Heptachlorobiphenyl 2,2',3,3',5,5',6,6'-Octachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. New York California State Department Department of Public of Health Health Lab ID 11674 NELAP Primary Lab ID 01138CA NELAP Secondary NP W S NP W S 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 State of Florida Department of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 77 77 77 77 77 77 7 77 77 77 77 77 77 77 77 77 77 State of New Jersey Department of Environmental Protection Lab ID CANA005 NELAP Secondary Dr. NP WW 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 S 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 7 T Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP W S 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 77 T 77 77 77 77 77 77 7 77 77 77 77 77 77 77 77 77 77 Page 211 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 1a PCB 205 PCB 206 PCB 208 PCB 209 Aroclor 1260 Aroclor 1254 Aroclor 1221 Aroclor 1232 Aroclor 1248 Aroclor 1016 Aroclor 1242 2,3,3',4,4',5,5',6-Octachlorobiphenyl 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl Decachlorobiphenyl Pesticides 4,4'-DDD 4,4'-DDE 4,4'-DDT Aldrin Alpha-HCH Beta-HCH cis-Chlordane (alpha-Chlordane) Chlordane, technical Delta-HCH Dieldrin Endosulphan I Endosulphan II Endosulphan sulphate Endrin Endrin aldehyde trans-Chlordane (gamma-Chlordane) Gamma-HCH (Lindane) Heptachlor Heptachlor epoxide Hexachlorobenzene Methoxychlor Mi rex New York California State Department Department of Public of Health Health Lab ID 11674 Lab ID 01138CA NELAP NELAP P rim a ry Secondary NP W S NP W S 7 7 7 7 7, 11 7, 11 7, 11 7, 11 7, 11 7, 11 7, 11 7 7 7 7 5, 7 5, 7 5, 7 5, 7 5, 7 5, 7 5, 7 11 11 11 11 11 11 11 5 5 5 5 5 5 5 State of Florida Department of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 11 5 55 5, 11 5 11 5 11 5 11 5 4646 4646 4646 4646 4646 4646 55 11 5 11 5 11 5 11 5 4646 21 5 9 5 4, 8 6 8 6 5 State of New Jersey Department of Environmental Protection Virginia Division of Consolidated Laboratory Services Lab ID C AN A005 NELAP Secondary Lab ID 460224 NELAP Secondary Dr. NP WW 7 7 7 7 S 7 7 7 7 T NP W S 77 77 77 77 11 5 11 5 11 5 11 5 11 5 11 5 11 5 T 11 11 11 11 11 11 5, 11 11 4 4 4 4 4 4 11 11 4 4 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 5 5 6 5 6 ACC-101 Rev 08, 08-Nov-2012 P ,ge4 o J l Page 212 of 234 www.axysanalytical.com TABLE 1a PAH Anthracene Pyrene Benzo[ghilperylene Indeno[1,2,3-cdlpyrene Benzo[blfluoranthene Fluoranthene Benzo[k]fluoranthene Acenaphthylene Chrysene Benzo[a]pyrene Dibenz[ahlanthracene Benz[alanthracene Acenaphthene Phenanthrene Fluorene Naphthalene AXYS Analytical Services Ltd. New York California State Department Department of Public of Health Health Lab ID 11674 NELAP Primary Lab ID 01138CA NELAP Secondary NP W S NP W S State of Florida Department of Health Lab ID E871007 NELAP Primary Dr. NP WW S T 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 State of New Jersey Department of Environmental Protection Lab ID CANA005 NELAP Secondary Dr. NP WW S T Virginia Division of Consolidated Laboratory Services Lab ID 460224 NELAP Secondary NP W S T 9 20 9 20 9 20 9 20 9 20 9 20 9 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 9 20 ACC-101 Rev 08, 08-Nov-2012 Pa9e 5 o J l Page 213 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Matrix Codes for Table 1b NPW = Non-Potable Water DrW = Drinking Water S = Solid T = Tissue Table 1b NELAP Accreditation Held by AXYS Analytical Services Ltd. for Perfluorinated Organic Compounds Accreditation Method Codes and Explanation for Table 1b Code Accreditation Certificate Applicable AXYS Method and Description No. Method Reference 12 AXYS MLA-041 13 AXYS MLA-043 14 AXYS MLA-060 MLA-041, laboratory performance based method (LC/MS-MS) MLA-043, laboratory performance based method (LC/MS-MS) MLA-060, laboratory performance based method (LC/MS-MS) TABLE 1b State of Florida Department of Health Lab ID E871007 NELAP Primary Dr. NP S WW T Minnesota Department of Health Lab ID 232-999-430 NELAP Primary! Dr. NP S T WW State of New Jersey Department of Environmental Protection Lab ID CANA005 NELAP Secondary Dr. NP S T WW PFC - Perfluorinated Organic Compounds Perfluorobutanoate (PFBA) Note Perfluoropentanoate (PFPeA) Note Perfluorohexanoate (PFHxA) Note Perfluoroheptanoate (PFHpA) Note Perfluorooctanoate (PFOA) Note Perfluorononanoate (PFNA) Note Perfluorodecanoate (PFDA) Note Perfluoroundecanoate (PFUnA) Note Perfluorododecanoate (PFDoA) Note Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 14 14 12 13 Note: Accreditations by Minnesota Department of Health and New Jersey Department of Environmental Protection are against the corresponding acid form of the anion shown. ACC-101 Rev 08, 08-Nov-2012 Page6ofC ^ Page 214 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Table 2: Canadian and US State Specific Accreditation Held by AXYS Analytical Services Ltd. Matrix Codes for Table 2 NP W = Non-Potable Water Dr. W = Drinking Water W = Aqueous S = Solid T = Tissue Ser = Serum/Plasma U = Urine Accreditation Method Codes and Explanation for Table 2 Code Accreditation Certificate No. Method Reference Applicable AXYS Method and Description 1 EPA 1613 3 AXYS MLA-017 7 EPA 1668A 10 AXYS MLA-007 12 AXYS MLA-041 13 AXYS MLA-043 14 AXYS MLA-060 15 AXYS MLA-010 16 AXYS MLA-028 17 AXYS MLA-033 18 AXYS MLA-021 19 AXYS MLA-075 22 AXYS MLA-901 23 AXYS MLA-042 24 AXYS MLA-070 25 AXYS MLA-079 26 AXYS MLA-059 27 AXYS MLA-064 28 EPA 1668C 29 AXYS MLA-021 MLA-017 Performance based implementation of EPA 1613B (GC/HRMS) MLA-017 Performance based implementation of EPA 1613B (g C/HRMs ) MLA-010 Performance based implementation of EPA 1668A (g C/HRMs ) MLA-007 Performance based modification of EPA 8270C/D, 8081A/B (GC/LRMS and GC/ECD) MLA-041 Laboratory performance based method (LC/MS-MS) MLA-043 Laboratory performance based method (LC/MS-MS) MLA-060 Laboratory performance based method (LC/MS-MS) MLA-010 Performance based implementation of EPA 1668A (GC/HRMS) MLA-028 Laboratory performance based method (GC/HRMS) MLA-033 Performance based implementation of EPA 1614 (Gc /HRMS) MLA-021 Performance based modification of EPA 8270C/D (GC/LRMS) MLA-075 Performance based implementation of EPA 1694 (LC/MS-MS) MLA-901 Laboratory performance based method (GC/LRMS) MLA-042 Laboratory performance based method (LC/MS-MS) MLA-070 Laboratory performance based method (LC/MS-MS) MLA-079 Laboratory performance based method (LC/MS-MS) MLA-059 Laboratory performance based method (LC/MS-MS) MLA-064 Laboratory performance based method (LC/MS-MS) MLA-010 Performance based implementation of EPA 1668C (GC/HRMS) MLA-021 Performance based modification of EPA 1625 (GC/LRMS) ACC-101 Rev 08, 08-Nov-2012 Pa9e 7 o J l Page 215 of 234 www.axysanalytical.com TABLE 2 PCDD/F - Polychlorinated Dioxins and Furans 1,2,3,4,6,7,8-HpCDD 1,2,3,4,6,7,8-HpCDF 1,2,3,4,7,8,9-HpCDF 1,2,3,4,7,8-HxCDD 1,2,3,4,7,8-HxCDF 1,2,3,6,7,8-HxCDD 1,2,3,6,7,8-HxCDF 1,2,3,7,8,9-HxCDD 1,2,3,7,8,9-HxCDF 1,2,3,7,8-PeCDD 1,2,3,7,8-PeCDF 2,3,4,6,7,8-HxCDF 2,3,4,7,8-PeCDF 2,3,7,8-TCDD 2,3,7,8-TCDF OCDD OCDF PCBs - Polychlorinated biphenyls PCB 1 2-Chlorobiphenyl PCB 2 3-Chlorobiphenyl PCB 3 4-Chlorobiphenyl PCB 4 2,2'-Dichlorobiphenyl PCB 5 2,3-Dichlorobiphenyl PCB 6 2,3'-Dichlorobiphenyl PCB 7 2,4-Dichlorobiphenyl PCB 8 2,4'-Dichlorobiphenyl PCB 8/5 PCB 9 2,5-Dichlorobiphenyl PCB 10 2,6-Dichlorobiphenyl PCB 11 3,3'-Dichlorobiphenyl PCB 12 3,4-Dichlorobiphenyl PCB 13 3,4'-Dichlorobiphenyl PCB 14 3,5-Dichlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 33333 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 11 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 10 10 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 15 15 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 P39e So J l Page 216 of 234 www.axysanalytical.com TABLE 2 PCB 15 4,4'-Dichlorobiphenyl PCB 16 2,2',3-T richlorobiphenyl PCB 16/32 PCB 17 2,2',4-T richlorobiphenyl PCB 18 2,2',5-Trichlorobiphenyl PCB 19 2,2',6-T richlorobiphenyl PCB 20 2,3,3'-Trichlorobiphenyl PCB 21 2,3,4-T richlorobiphenyl PCB 22 2,3,4'-Trichlorobiphenyl PCB 23 2,3,5-T richlorobiphenyl PCB 24 2,3,6-T richlorobiphenyl PCB 24/27 PCB 25 2,3',4-T richlorobiphenyl PCB 26 2,3',5-Trichlorobiphenyl PCB 27 2,3',6-Trichlorobiphenyl PCB 28 2,4,4'-Trichlorobiphenyl PCB 29 2,4,5-T richlorobiphenyl PCB 30 2,4,6-T richlorobiphenyl PCB 31 2,4',5-Trichlorobiphenyl PCB 32 2,4',6-Trichlorobiphenyl PCB 33 2,3',4'-Trichlorobiphenyl PCB 33/20/21 PCB 34 2,3',5'-Trichlorobiphenyl PCB 35 3,3',4-Trichlorobiphenyl PCB 36 3,3',5-Trichlorobiphenyl PCB 37 3,4,4'-Trichlorobiphenyl PCB 38 3,4,5-T richlorobiphenyl PCB 39 3,4',5-Trichlorobiphenyl PCB 40 2,2',3,3'-Tetrachlorobiphenyl PCB 41 2,2',3,4-Tetrachlorobiphenyl PCB 41/71/64/68 PCB 42 2,2',3,4'-Tetrachlorobiphenyl PCB 42/59 PCB 43 2,2',3,5-Tetrachlorobiphenyl PCB 44 2,2',3,5'-Tetrachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 W S Pulp T Ser 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 18 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 10 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 10, 15 15 10 10, 15 10, 15 10, 15 15 15 10, 15 15 15 10 10, 15 10, 15 15 10, 15 15 15 10, 15 15 15 10 15 15 15 15 15 15 10, 15 15 10 15 10 15 10, 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 Washington State Department of Ecology Lab. ID: C404 U NP S W 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 P39e 9oJ l Page 217 of 234 www.axysanalytical.com TABLE 2 PCB 45 PCB 46 PCB 47 PCB 47/48/75 PCB 48 PCB 49 PCB 49/43 PCB 50 PCB 51 PCB 52 PCB 52/73 PCB 53 PCB 54 PCB 55 PCB 56 PCB 56/60 PCB 57 PCB 58 PCB 59 PCB 60 PCB 61 PCB 62 PCB 63 PCB 64 PCB 65 PCB 66 PCB 66/80 PCB 67 PCB 68 PCB 69 PCB 70 PCB 70/76 PCB 71 PCB 72 PCB 73 2,2',3,6-Tetrachlorobiphenyl 2,2',3,6'-Tetrachlorobiphenyl 2,2',4,4'-Tetrachlorobiphenyl 2,2',4,5-Tetrachlorobiphenyl 2,2',4,5'-Tetrachlorobiphenyl 2,2',4,6-Tetrachlorobiphenyl 2,2',4,6'-Tetrachlorobiphenyl 2,2',5,5'-Tetrachlorobiphenyl 2,2',5,6'-Tetrachlorobiphenyl 2,2',6,6'-Tetrachlorobiphenyl 2,3,3',4-Tetrachlorobiphenyl 2,3,3',4'-Tetrachlorobiphenyl 2,3,3',5-Tetrachlorobiphenyl 2,3,3',5'-Tetrachlorobiphenyl 2,3,3',6-Tetrachlorobiphenyl 2,3,4,4'-Tetrachlorobiphenyl 2,3,4,5-Tetrachlorobiphenyl 2,3,4,6-Tetrachlorobiphenyl 2,3,4',5-Tetrachlorobiphenyl 2,3,4',6-Tetrachlorobiphenyl 2,3,5,6-Tetrachlorobiphenyl 2,3',4,4'-Tetrachlorobiphenyl 2,3',4,5-Tetrachlorobiphenyl 2,3',4,5'-Tetrachlorobiphenyl 2,3',4,6-Tetrachlorobiphenyl 2,3',4',5-Tetrachlorobiphenyl 2,3',4',6-Tetrachlorobiphenyl 2,3',5,5'-Tetrachlorobiphenyl 2,3',5',6-Tetrachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 W S Pulp T Ser 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 10, 15 10, 15 15 10 15 15 10 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 10 15 15 15 15 10 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 15 Washington State Department of Ecology Lab. ID: C404 U NP S W 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 28 Page 10 of-mJPage 218 of 234 www.axysanalytical.com TABLE 2 PCB 74 2,4,4',5-Tetrachlorobiphenyl PCB 74/61 PCB 75 2,4,4',6-Tetrachlorobiphenyl PCB 76 2,3',4',5'-Tetrachlorobiphenyl PCB 77 3,3',4,4'-Tetrachlorobiphenyl PCB 78 3,3',4,5-Tetrachlorobiphenyl PCB 79 3,3',4,5'-Tetrachlorobiphenyl PCB 80 3,3',5,5'-Tetrachlorobiphenyl PCB 81 3,4,4',5-Tetrachlorobiphenyl PCB 82 2,2',3,3',4-Pentachlorobiphenyl PCB 83 2,2',3,3',5-Pentachlorobiphenyl PCB 83/108 PCB 84 2,2',3,3',6-Pentachlorobiphenyl PCB 85 2,2',3,4,4'-Pentachlorobiphenyl PCB 85/120 PCB 86 2,2',3,4,5-Pentachlorobiphenyl PCB 87 2,2',3,4,5'-Pentachlorobiphenyl PCB 87/115/116 PCB 88 2,2',3,4,6-Pentachlorobiphenyl PCB 89 2,2',3,4,6'-Pentachlorobiphenyl PCB 90 2,2',3,4',5-Pentachlorobiphenyl PCB 91 2,2',3,4',6-Pentachlorobiphenyl PCB 92 2,2',3,5,5'-Pentachlorobiphenyl PCB 93 2,2',3,5,6-Pentachlorobiphenyl PCB 94 2,2',3,5,6'-Pentachlorobiphenyl PCB 95 2,2',3,5',6-Pentachlorobiphenyl PCB 95/93 PCB 96 2,2',3,6,6'-Pentachlorobiphenyl PCB 97 2,2',3,4',5'-Pentachlorobiphenyl PCB 97/86 PCB 98 2,2',3,4',6'-Pentachlorobiphenyl PCB 99 2,2',4,4',5-Pentachlorobiphenyl PCB 100 2,2',4,4',6-Pentachlorobiphenyl PCB 101 2,2',4,5,5'-Pentachlorobiphenyl PCB 101/90/89 ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 15 15, 22 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 Page 11 of-mJPage 219 of 234 www.axysanalytical.com TABLE 2 PCB 102 PCB 103 PCB 104 PCB 105 PCB 105/127 PCB 106 PCB 107 PCB 107/109 PCB 108 PCB 109 PCB 110 PCB 111 PCB 112 PCB 113 PCB 114 PCB 115 PCB 116 PCB 117 PCB 118 PCB 118/116 PCB 119 PCB 120 PCB 121 PCB 122 PCB 123 PCB 124 PCB 125 PCB 126 PCB 127 PCB 128 PCB 129 PCB 130 PCB 131 PCB 131/142 PCB 132 2,2',4,5,6'-Pentachlorobiphenyl 2,2',4,5',6-Pentachlorobiphenyl 2,2',4,6,6'-Pentachlorobiphenyl 2,3,3',4,4'-Pentachlorobiphenyl 2,3,3',4,5-Pentachlorobiphenyl 2,3,3',4',5-Pentachlorobiphenyl 2,3,3',4,5'-Pentachlorobiphenyl 2,3,3',4,6-Pentachlorobiphenyl 2,3,3',4',6-Pentachlorobiphenyl 2,3,3',5,5'-Pentachlorobiphenyl 2,3,3',5,6-Pentachlorobiphenyl 2,3,3',5',6-Pentachlorobiphenyl 2,3,4,4',5-Pentachlorobiphenyl 2,3,4,4',6-Pentachlorobiphenyl 2,3,4,5,6-Pentachlorobiphenyl 2,3,4',5,6-Pentachlorobiphenyl 2,3',4,4',5-Pentachlorobiphenyl 2,3',4,4',6-Pentachlorobiphenyl 2,3',4,5,5'-Pentachlorobiphenyl 2,3',4,5',6-Pentachlorobiphenyl 2,3,3',4',5'-Pentachlorobiphenyl 2,3',4,4',5'-Pentachlorobiphenyl 2,3',4',5,5'-Pentachlorobiphenyl 2,3',4',5',6-Pentachlorobiphenyl 3,3',4,4',5-Pentachlorobiphenyl 3,3',4,5,5'-Pentachlorobiphenyl 2,2',3,3',4,4'-Hexachlorobiphenyl 2,2',3,3',4,5-Hexachlorobiphenyl 2,2',3,3',4,5'-Hexachlorobiphenyl 2,2',3,3',4,6-Hexachlorobiphenyl 2,2',3,3',4,6'-Hexachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15, 22 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 mPage 12 of-JPage 220 of 234 www.axysanalytical.com TABLE 2 PCB 133 2,2',3,3',5,5'-Hexachlorobiphenyl PCB 134 2,2',3,3',5,6-Hexachlorobiphenyl PCB 134/143 PCB 135 2,2',3,3',5,6'-Hexachlorobiphenyl PCB 136 2,2',3,3',6,6'-Hexachlorobiphenyl PCB 137 2,2',3,4,4',5-Hexachlorobiphenyl PCB 138 2,2',3,4,4',5'-Hexachlorobiphenyl PCB 138/163/164 PCB 139 2,2',3,4,4',6-Hexachlorobiphenyl PCB 140 2,2',3,4,4',6'-Hexachlorobiphenyl PCB 141 2,2',3,4,5,5'-Hexachlorobiphenyl PCB 142 2,2',3,4,5,6-Hexachlorobiphenyl PCB 143 2,2',3,4,5,6'-Hexachlorobiphenyl PCB 144 2,2',3,4,5',6-Hexachlorobiphenyl PCB 144/135 PCB 145 2,2',3,4,6,6'-Hexachlorobiphenyl PCB 146 2,2',3,4',5,5'-Hexachlorobiphenyl PCB 147 2,2',3,4',5,6-Hexachlorobiphenyl PCB 148 2,2',3,4',5,6'-Hexachlorobiphenyl PCB 149 2,2',3,4',5',6-Hexachlorobiphenyl PCB 149/139 PCB 150 2,2',3,4',6,6'-Hexachlorobiphenyl PCB 151 2,2',3,5,5',6-Hexachlorobiphenyl PCB 152 2,2',3,5,6,6'-Hexachlorobiphenyl PCB 153 2,2',4,4',5,5'-Hexachlorobiphenyl PCB 154 2,2',4,4',5,6'-Hexachlorobiphenyl PCB 155 2,2',4,4',6,6'-Hexachlorobiphenyl PCB 156 2,3,3',4,4',5-Hexachlorobiphenyl PCB 157 2,3,3',4,4',5'-Hexachlorobiphenyl PCB 158 2,3,3',4,4',6-Hexachlorobiphenyl PCB 158/160 PCB 159 2,3,3',4,5,5'-Hexachlorobiphenyl PCB 160 2,3,3',4,5,6-Hexachlorobiphenyl PCB 161 2,3,3',4,5',6-Hexachlorobiphenyl PCB 162 2,3,3',4',5,5'-Hexachlorobiphenyl ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15, 22 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 mPage 13 of-JPage 221 of 234 www.axysanalytical.com TABLE 2 PCB 163 PCB 164 PCB 165 PCB 166 PCB 167 PCB 168 PCB 169 PCB 170 PCB 170/190 PCB 171 PCB 172 PCB 172/192 PCB 173 PCB 174 PCB 174/181 PCB 175 PCB 176 PCB 177 PCB 178 PCB 179 PCB 180 PCB 181 PCB 182 PCB 183 PCB 184 PCB 185 PCB 186 PCB 187 PCB 187/182 PCB 188 PCB 189 PCB 190 PCB 191 PCB 192 PCB 193 2,3,3',4',5,6-Hexachlorobiphenyl 2,3,3',4',5',6-Hexachlorobiphenyl 2,3,3',5,5',6-Hexachlorobiphenyl 2,3,4,4',5,6-Hexachlorobiphenyl 2,3',4,4',5,5'-Hexachlorobiphenyl 2,3',4,4',5',6-Hexachlorobiphenyl 3,3',4,4',5,5'-Hexachlorobiphenyl 2,2',3,3',4,4',5-Heptachlorobiphenyl 2,2',3,3',4,4',6-Heptachlorobiphenyl 2,2',3,3',4,5,5'-Heptachlorobiphenyl 2,2',3,3',4,5,6-Heptachlorobiphenyl 2,2',3,3',4,5,6'-Heptachlorobiphenyl 2,2',3,3',4,5',6-Heptachlorobiphenyl 2,2',3,3',4,6,6'-Heptachlorobiphenyl 2,2',3,3',4,5',6'-Heptachlorobiphenyl 2,2',3,3',5,5',6-Heptachlorobiphenyl 2,2',3,3',5,6,6'-Heptachlorobiphenyl 2,2',3,4,4',5,5'-Heptachlorobiphenyl 2,2',3,4,4',5,6-Heptachlorobiphenyl 2,2',3,4,4',5,6'-Heptachlorobiphenyl 2,2',3,4,4',5',6-Heptachlorobiphenyl 2,2',3,4,4',6,6'-Heptachlorobiphenyl 2,2',3,4,5,5',6-Heptachlorobiphenyl 2,2',3,4,5,6,6'-Heptachlorobiphenyl 2,2',3,4',5,5',6-Heptachlorobiphenyl 2,2',3,4',5,6,6'-Heptachlorobiphenyl 2,3,3',4,4',5,5'-Heptachlorobiphenyl 2,3,3',4,4',5,6-Heptachlorobiphenyl 2,3,3',4,4',5',6-Heptachlorobiphenyl 2,3,3',4,5,5',6-Heptachlorobiphenyl 2,3,3',4',5,5',6-Heptachlorobiphenyl AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15, 22 28 28 10 10 10 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10 10 10 15 15 15 15 28 28 15 15 15 15 28 28 10 10 10 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 10, 15 10, 15 10, 15 15, 22 28 28 15 15 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 15 15 15 15, 22 28 28 10 10 10 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 15 15 15 15 28 28 10, 15 10, 15 10, 15 15 28 28 ACC-101 Rev 08, 08-Nov-2012 mPage 14 of-JPage 222 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 Washington State Department of Ecology Lab. ID: C404 W S Pulp T Ser U NP S W PCB 194 2,2',3,3',4,4',5,5'-Octachlorobiphenyl 10, 15 10, 15 10, 15 15, 22 28 28 PCB 195 2,2',3,3',4,4',5,6-Octachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 196 2,2',3,3',4,4',5,6-Octachlorobiphenyl 15 15 15 15 28 28 PCB 196/203 10 10 10 PCB 197 2,2',3,3',4,4',6,6'-Octachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 198 2,2',3,3',4,5,5',6-Octachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 199 2,2',3,3',4,5,5',6'-Octachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 200 2,2',3,3',4,5,6,6'-Octachlorobiphenyl 15 15 15 15 28 28 PCB 201 2,2',3,3',4,5',6,6'-Octachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 202 2,2',3,3',5,5',6,6-Octachlorobiphenyl 15 15 15 15 28 28 PCB 203 2,2',3,4,4',5,5',6-Octachlorobiphenyl 15 15 15 15 28 28 PCB 204 2,2',3,4,4',5,6,6'-Octachlorobiphenyl 15 15 15 15 28 28 PCB 205 2,3,3',4,4',5,5',6-Octachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 206 2,2',3,3',4,4',5,5',6-Nonachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 207 2,2',3,3',4,4',5,6,6'-Nonachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 208 2,2',3,3',4,5,5',6,6'-Nonachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 PCB 209 Decachlorobiphenyl 10, 15 10, 15 10, 15 15 28 28 Total Monochlorobiphenyls 15 15 15 15 28 28 Total Dichlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Trichlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Tetrachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Pentachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Hexachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Heptachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Octachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Nonachlorobiphenyls 10, 15 10, 15 10, 15 15 28 28 Total Decachlorobiphenyls 10 10 10 Total Polychlorinated biphenyls 10 10 10 A ro c lo rs Aroclor 1260 Aroclor 1254 Aroclor 1268 Aroclor 1221 Aroclor 1232 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 ACC-101 Rev 08, 08-Nov-2012 Page 15 of-mJPage 223 of 234 www.axysanalytical.com TABLE 2 Aroclor 1248 Aroclor 1016 Aroclor 1242 Aroclor 1242/1016 Pesticides 2,4'-DDD 2,4'-DDE 2,4'-DDT 4,4'-DDD 4,4'-DDE 4,4'-DDT Aldrin Alpha-HCH Beta-HCH cis-Chlordane (alpha-Chlordane) cis-Nonachlor Delta-HCH Dieldrin Endosulphan I Endosulphan II Endosulphan sulphate Endrin Endrin aldehyde Endrin ketone Gamma-HCH (Lindane) Heptachlor Heptachlor epoxide Hexachlorobenzene Hexachlorobutadiene Methoxychlor Mirex Oxychlordane Toxaphene trans-Chlordane (gamma-Chlordane) ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 10 10 10 10 10 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 10, 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 16 Page 16 of-mJPage 224 of 234 www.axysanalytical.com TABLE 2 trans-Nonachlor BDE - Brominated Diphenylethers BDE 7 2,4-dibromodiphenylether BDE 8 2,4'-dibromodiphenylether BDE 10 2,6-dibromodiphenylether BDE 11 3,3'-dibromodiphenylether BDE 12 3,4-dibromodiphenylether BDE 13 3,4'-dibromodiphenylether BDE 15 4,4'-dibromodiphenylether BDE 17 2,2',4-tribromodiphenylether BDE 25 2,3',4-tribromodiphenylether BDE 28 2,4,4'-tribromodiphenylether BDE 30 2,4,6-tribromodiphenylether BDE-33 2',3,4-tribromodiphenylether BDE 35 3,3',4-tribromodiphenylether BDE 37 3,4,4'-tribromodiphenylether BDE 47 2,2',4,4'-tetrabromodiphenylether BDE 49 2,2',4,5'-tetrabromodiphenylether BDE 66 2,3',4,4'-tetrabromodiphenylether BDE 75 2,4,4',6-tetrabromodiphenylether BDE 77 3,3',4,4'-tetrabromodiphenylether BDE 85 2,2',3,4,4'-pentabromodiphenylether BDE 99 2,2',4,4',5-pentabromodiphenylether BDE 100 2,2',4,4',6-pentabromodiphenylether BDE 105 2,3,3',4,4'-pentabromodiphenylether BDE 116 2,3,4,5,6-pentabromodiphenylether BDE 119 2,3',4,4',6-pentabromodiphenylether BDE 126 3,3',4,4',5-pentabromodiphenylether BDE 140 2,2',3,4,4',6'-hexabromodiphenylether BDE 153 2,2',4,4',5,5'-hexabromodiphenylether BDE 154 2,2',4,4',5',6-hexabromodiphenylether BDE 155 2,2',4,4',6,6'-hexabromodiphenylether BDE 166 2,3,4,4',5,6-hexabromodiphenylether BDE 181 2,2',3,4,4',5,6-heptabromodiphenylether ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U 16 10, 16 10, 16 10, 16 NP W 16 S 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 mPage 17 of-JPage 225 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 BDE-183 BDE 190 BDE 206 BDE 207 BDE 208 BDE 209 2,2',3,4,4',5',6-heptabromodiphenylether 2,3,3',4,4',5,6-heptabromodiphenylether 2,2',3,3',4,4',5,5',6-nonabromodiphenylether 2,2',3,3',4,4',5,6,6'-nonabromodiphenylether 2,2',3,3',4,5,5',6,6'-nonabromodiphenylether Decabromodiphenylether PFC - Perfluorinated O rganic Com pounds Perfluorobutanoate (PFBA) Perfluoropentanoate (PFPeA) Perfluorohexanoate (PFHxA) Perfluoroheptanoate (PFHpA) Perfluorooctanoate (PFOA) Perfluorononanoate (PFNA) Perfluorodecanoate (PFDA) Perfluoroundecanoate (PFUnA) Perfluorododecanoate (PFDoA) Perfluorobutanesulfonate (PFBS) Perfluorohexanesulfonate (PFHxS) Perfluorooctanesulfonate (PFOS) Perfluorooctane sulfonamide (PFOSA) PAH Anthracene Pyrene Benzo[ghi]perylene Benzo[e]pyrene Indeno[1,2,3-cd]pyrene Perylene Benzo[b]fluoranthene Fluoranthene Benzo[j/k]fluoranthenes Benzo[k]fluoranthene Acenaphthylene Chrysene W 17 17 17 17 17 17 14 14 14 14 14 14 14 14 14 14 14 14 14 29 29 29 29 29 29 29 29 29 29 29 29 Canadian Association for Laboratory Accreditation (CALA) Accreditation no.: A 2637 S Pulp T Ser 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 17 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 12 13 23 29 18 29 18 29 18 29 18 29 18 29 18 29 18 29 18 18 29 18 29 18 Washington State Department of Ecology Lab. ID: C404 U NP W 17 17 17 17 17 17 S ACC-101 Rev 08, 08-Nov-2012 Page 18 of-mJPage 226 of 234 www.axysanalytical.com TABLE 2 Benzo[a]pyrene Dibenz[ah]anthracene Benz[a]anthracene Acenaphthene Phenanthrene Fluorene Naphthalene 1-Methylchrysene 1-Methylnaphthalene 1-Methylphenanthrene 1,2-Dimethylnaphthalene 1,2,6-T rimethylphenanthrene 1,4,6,7-Tetramethylnaphthalene 1,7-Dimethylfluorene 1,7-Dimethylphenanthrene 1,8-Dimethylphenanthrene 2-Methylanthracene 2-Methylfluorene 2-Methylnaphthalene 2-Methylphenanthrene 2/3-Methyldibenzothiophenes 2,3,5-T rimethylnaphthalene 2,3,6-T rimethylnaphthalene 2,4-Dimethyldibenzothiophene 2,6-Dimethylnaphthalene 2,6-Dimethylphenanthrene 3-Methylfluoranthene/ Benzo(a)fluorene 3-Methylphenanthrene 3,6-Dimethylphenanthrene 5/6-Methylchrysenes 5,9-Dimethylchrysene 7-Methylbenzo(a)pyrene 9/4-Methylphenanthrenes Biphenyl C1-Acenaphthenes ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 29 29 18 29 29 18 29 29 18 29 29 18 29 29 18 29 29 18 29 29 18 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 Page 19 of-mJPage 227 of 234 www.axysanalytical.com TABLE 2 C1-Benz(a)anthracenes/chrysenes C1-Benzofluoranthenes/ Benzopyrenes C1-Biphenyls C1-Dibenzothiophene C1-Fluoranthenes/Pyrenes C1-Fluorenes C1-Naphthalenes C1-Phenanthrenes/Anthracenes C2-Benz(a)anthracenes/Chrysenes C2-Benzofluoranthenes/ Benzopyrenes C2-Biphenyls C2-Dibenzothiophene C2-Fluoranthenes/Pyrenes C2-Fluorenes C2-Naphthalenes C2-Phenanthrenes/Anthracenes C3-Benz(a)anthracenes/Chrysenes C3-Dibenzothiophene C3-Fluoranthenes/Pyrenes C3-Fluorenes C3-Naphthalenes C3-Phenanthrenes/Anthracenes C4-Benz(a)anthracenes/Chrysenes C4-Dibenzothiophene C4-Fluoranthenes/Pyrenes C4-Naphthalenes C4-Phenanthrenes/Anthracenes Dibenzothiophene Retene PPCP (Pharmaceutical and Personal Care Products) Acetaminophen Azithromycin Caffeine Carbadox AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 29 19 19 19 19 19 19 19 19 ACC-101 Rev 08, 08-Nov-2012 Page 20 of-mJPage 228 of 234 www.axysanalytical.com TABLE 2 Carbamazepine Cefotaxime Ciprofloxacin Clarithromycin Clinafloxacin Cloxacillin Dehydronifedipine Digoxigenin Digoxin Diltiazem 1,7-Dimethylxanthine Diphenhydramine Enrofloxacin Erythromycin Flumequine Fluoxetine Lincomycin Lomefloxacin Miconazole Norfloxacin Norgestimate Ofloxacin Ormetoprim Oxacillin Oxolinic acid Penicillin G Penicillin V Roxithromycin Sarafloxacin Sulfachloropyridazine Sulfadiazine Sulfadimethoxine Sulfamerazine Sulfamethazine Sulfamethizole ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 21 of-mJPage 229 of 234 www.axysanalytical.com TABLE 2 Sulfamethoxazole Sulfanilamide Sulfathiazole Thiabendazole Trimethoprim Tylosin Virginiamycin Anhydrochlortetracycline (ACTC) Anhydrotetracycline (ATC) Chlortetracycline (CTC) Demeclocycline Doxycycline 4-Epianhydrochlortetracycline (EACTC) 4-Epianhydrotetracycline (EATC) 4-Epichlortetracycline (ECTC) 4-Epioxytetracycline (EOTC) 4-Epitetracycline (ETC) Isochlortetracycline (ICTC) Minocycline Oxytetracycline (OTC) Tetracycline (TC) Bisphenol A Furosemide Gemfibrozil Glipizide Glyburide Hydrochlorothiazide 2-hydroxy-ibuprofen Ibuprofen Naproxen Triclocarban Triclosan Warfarin ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 22 o f .mJ Page 230 of 234 www.axysanalytical.com TABLE 2 Albuterol Amphetamine Atenolol Atorvastatin Cimetidine Clonidine Codeine Cotinine Enalapril Hydrocodone Metformin Oxycodone Ranitidine Triamterene Alprazolam Amitriptyline Amlodipine Benzoylecgonine Benztropine Betamethasone Cocaine DEET (N,N-diethyl-m-toluamide) Desmethyldiltiazem Diazepam Fluocinonide Fluticasone propionate Hydrocortisone 10-hydroxy-amitriptyline Meprobamate Methylprednisolone Metoprolol Norfluoxetine Norverapamil ACC-101 Rev 08, 08-Nov-2012 AXYS Analytical Services Ltd. Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 Page 23 o f .mJ Page 231 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Paroxetine Prednisolone Prednisone Promethazine Propoxyphene Propranolol Sertraline Simvastatin Theophylline Trenbolone Trenbolone acetate Valsartan Verapamil HBCDD alpha-hexabromocyclododecane (a-HBCDD) beta-hexabromocyclododecane (b-HBCDD) gamma-hexabromocyclododecane (g-HBCDD) TBBPA Tetrabromobisphenol A BPA and MPE 4,4'-dihydroxy-2,2-diphenylpropane (Bisphenol A) (BPA) Mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP) Mono-(2-ethyl-5-oxohexyl) phthalate (MEOHP) Mono-(3-carboxypropyl) phthalate (MCPP) Mono-2-ethylhexyl phthalate (MEHP) Mono-benzyl phthalate (MBzP) Mono-butyl phthalate (MBP) (n + iso) Mono-cyclohexyl phthalate (MCHP) Mono-ethyl phthalate (MEP) Mono-iso-nonyl phthalate (MiNP) Mono-methyl phthalate (MMP) Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 24 24 24 25 26 26 26 26 26 26 26 26 26 26 26 ACC-101 Rev 08, 08-Nov-2012 Page 24 of-mJPage 232 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. TABLE 2 Canadian Association for Laboratory Accreditation (CALA) Washington State Department of Ecology Accreditation no.: A 2637 Lab. ID: C404 W S Pulp T Ser U NP S W Parabens Benzylparaben (Benzyl 4-hydroxybenzoate) Butylparaben (n-Butyl 4-hydroxybenzoate and isobutyl 4hydroxybenzoate) Ethylparaben (Ethyl 4-hydroxybenzoate) Isopropylparaben (Isopropyl 4-hydroxybenzoate) Methylparaben (Methyl 4-hydroxybenzoate) n-Propylparaben (Propyl 4-hydroxybenzoate) 27 27 27 27 27 27 Table 1 and Table 2 - Explanation of Terms Used: NELAP = National Environmental Laboratory Accreditation Program Non-potable water = water not fit for consumption without treatment as it may contain pollutants, contaminants, minerals or infective agents. Surface water, ground water, rainwater, effluents as well as any other non-drinking water sources are included in this category. Solid = environmental solid sample. Soil, sediment, biosolids, hazardous waste, mixed phase samples with significant solids content are included in this category. Performance based implementation = methodology follows that of the method reference but modifications deemed by AXYS as minor 1may apply, results meet method reference data quality standard. Performance based modification = modifications deemed by AXYS as significant2 have been made to method reference protocol, results meet method reference accuracy standard. The suitability of the methodology for any method prescriptive applications should be assessed based on the modifications made and the specific work requirements. Performance based method = an in-house AXYS method, published method reference not applicable. GC/LRMS = gas chromatography, low resolution mass spectrometry detection. GC/HRMS = gas chromatography, high resolution mass spectrometry detection. GC/ECD = gas chromatography, electron capture detection. LC/MS-MS = liquid chromatography, mass spectrometry-mass spectrometry detection. ACC-101 Rev 08, 08-Nov-2012 Page 25 o f .mJ Page 233 of 234 www.axysanalytical.com AXYS Analytical Services Ltd. Note 1: Performance Based Implementation - Examples of Minor Modifications - use of additional isotopically labeled references - adjustment of calibration range - adjustment of clean-up technique - use of a different extraction of same general type (example soxhlet vs soxhlet Dean Stark) - addition of matrix type using same principles (example addition of tissue matrix using same detection principle and similar extraction type) Note 2: Performance Based Modification - Examples of Significant Modifications - different acquisition conditions using same detection principle (example MS SIM vs. full scan) - different internal control limits while meeting method reference accuracy standard ACC-101 Rev 08, 08-Nov-2012 mPage 26 of S ' Page 234 of 234