Document 6wvj9Q9me5Ln6wxqkmeVmJ7Rd

KETFERING LABORATORY EDEN AND BETHE5DA AVENUES CINCINNATI. OHIO <45218 UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE DEPARTMENT OP ENVIRONMENTAL HEALTH July 26, 1967 Dr. Robert J.M. Horton Health Effects Research Program Public Health Service 5008 Federal Office Bldg. Cincinnati, Ohio 45202 ___ Dear Dr. Horton: Last week's discussions identified certain areas of the proposed lead study which require modification. The groups felt that repeated aerbmetric measurements should be conducted at all ,, "Tri-City" sites (except Philadelphia Airport) and that the cadaver study should not be considered at this time. Neither of these changes materially changes the scope of the work, although full time coverage of sampling sites will become essential. In response to other specific comments, our response would be as follows: 1) Timetable: We believe that the original three cities can be resurveyed in the year running from late 1967 or early 1968. The actual time of initiating the surveys will reflect the rate of progress in re-establishing the original stations and obtaining suitable itioni torin g personnel. It is not necessary that the entire experimental protocol be established prior to the start of this sampling program. During the following year the stations will be moved to new sites in Chicago, New York, and the Washington area. Sampling will be undertaken in 1968 to confirm the readings made at low-lead NASA's, sites (such as Cedar Rapids) and to examine the advisability of air and biological studies there in 1969. 196 8 (old) 1969 (new) Cincinnati Los Angeles Philadelphia ? Cedar Rapids (pilot) 1 or 2 -4 -8 -7 20 New York Chicago Washington (& suburban) ? Cedar Rapids -6 -6 -6 -2 2) Aerometric sample pooling: To avoid obscuring transient high weekly lead readings in a monthly pool, pooling on a weekly basis will be regarded as the standard procedure. 3) Biological sampling: This will be conducted essentially as proposed with duplicate analyses of blood specimens. Early during the start-up period, the suitability of "old sites" for biological correlations will be established. In all probability Dr. Robert J.M. Horton Page - 2 some of these will be representative of a surrounding residential community and others will not. Four stations in Cincinnati, four in Los hngel.es, and perhaps two in Philadelphia are desirable for biological measurements and correlations. The extent to which these ten stations are included in the original nineteen will be determined after the re-establishment of the "Tri-City" sites. For planning purposes we will assume that 8 additional sites will be required. 4) Hematocrit factors: Although the effects of hemoglobin levels in population groups will probably not be of significance, microhematocrits will be determined at the time of each blood collection. 5) Control blood specimens: Three rather than two con trol levels will be utilized and will be available for inter laboratory comparisons. A nurse at the Cincinnati General Hospital has been selected to carry out the program. She will have custody of the tubes and will introduce them into the analytical process according to a schedule developed by the statistical group. Her post in the hospital will permit her to have correspondence with outside laboratories and access to the Laboratory on a sufficiently regular basis so that control specimens will not be obvious. 6) Tube and filter controls: Batch checks will be con ducted to assure continued freedom from contai?Tation. 7) Correlation of old and proposed aerometric analyses: The Laboratory will analyze on a "blind" basis filter wedges re tained from the original study. Volume of Samples A. Original sites 1) Cincinnati - 4 stations x 1 pool/wk x 56 wks =224 2) Los Angeles -8 "x 1 " " x 56 " =448 3) Philadelphia - 7 "x 1 " " x 56 " = 392 1064 (Most filters will be changed on a 2 - 3 x/wk basis. In high filter load areas in Los Angeles, tandem pumps yield ing 7 filters/week will be used.) UK 0018525 Dr. Robert J.M. Horton Page - 3 B. New sites 1} Cedar Rapids (or equivalent NASH low-lead, pending ' confirmation; pilot studies at various stations) 2 stations x 1 pool/wk. x 56 wks. = 112 2) Neighborhood stations (assuming 8 in addition to above) 8 stations x 1pool/wk. x 56 wks. = 448 C. Blood Specimens C to be run in duplicate) 10 geographical (neighborhood) groups x 100 persons = 1000 10 occupational groups x 50 persons = 500 D. Metabolic Specimens 6 groups x 10 persons/group x 10 days x 2 specimens/day = 1200 specimens E. Controls, rechecks, and misc 500 specimens We shall of course be pleased to discuss details of this work with you at any time. Very truly yours LBT/lh Lloyd B. Tepper, M.D Associate Director K5* 0018526