Document 6wE8dMoOLDRwKyg3n5ZDQjjkE
FOR DU PONT USE ONLY
AR226-2763
S. I. du Pone de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine
Elkton Road, Newark, Delaware 19711
HASKELL LABORATORY REPORT NO. 291-81
Material Tested Octanolc acid, pentadecaflucro-, ammonium salt*
Haskell No.
Other Codes
Study Initiated/Completed 5720/80 - 6/17/81 "
Material Submitted by G. L. Kennedy CR&D Department Haskell Laboratory
ORAL LB50 TEST IN GUINEA PIGS
Procedures The teat material, as a suspension in c o m oil, was administered by intragas trie intubation in single doses to 6 groups of male and 6 groups of female young adult Duncan Hartley guinea pigs, 10 per group. The
surviving guinea pigs were weighed and observed during a 14-day recovery period and then sacrificed. The LB5Q value was calculated from the mortality data using the method of D. J. Finney.**
Results:- ' "
"
-- -- ---- -- -- -- -- -- --
Males Dose
(mg/kg) 0
400
300
250
200
150
Average Body Weight g) 419 492 522 433 461 425
Suspension Average (T) Dose (ml) 10 2.80 10 1.97 5 3.13 5 * 2.15 5 2.13 2 3.19
Mortality Ratio 10/10 10/10 10/10 9/10 6/10 3/10
LD50*** 178 mg/kg
- 1-
Company Sanitized. Does not contain TS C A CBI
Results; (Cont'd.)
Females Doee
(ng/kg)
Avertge Body Height (g)
Suspension (X)
Average Dose (nl)
Mortality Ratio
670 450
10
3.02
10/10
400 499
10
2.00
10/10
300 477
5 2.86 9/10
LD50*** 217 mg/kg
250 419
5 2.10 8/10
200 493
5 1.97 1/10
150 411
2 3.09 2/10
Clinical Signs:
Males: Stained and/or wet perineal area, weakness at all levels tested except 670 and 400 mg/kg.
Stained face at 670, 200 and 150 og/kg.
Eyes half closed at 300 and 200 ng/kg.
Lacrlmatlon at 200 and 150 mg/kg.
Salivation at 300 and 150 ng/kg.
Ataxia at 670 mg/kg.
Tremors at 150 mg/kg.
Humped posture at 200 ng/kg.
Height loss at all levels tp :d except at 670 and 400 mg/kg.
All deaths occurred within 1-4 days after dosing.
2- -
;ompa3f Sanitized. Dues no
TSCA C/i<
Clinical Sign; (Cont'd.) Females r Stained and/or wee perineal area and weakness ae all levels tested except 670 and 400 mg/kg. Convulsions, ataxia, gasping ae 670 mg/kg Eyes half closed at 250 and 150 mg/kg. Tremors at 400 mg/kg. Lacrimatlon at 250 og/kg. Stained face at 200 mg/kg. Lethargy at 150 mg/kg. Height loss at all levels tested except for 670 mg/kg. All deaths occurred within 1-6 days after dosing.
Summary;
moderately toxic when administered
orally to y o u n ^ a a u ^ ^ M H H H i ^ ^ ^ ^ E a l e and female guinea pigs; its LD50
for males is 178 mg/kg and females 217 mg/kg of body weight. Clinical signs
for smiles and females Included: stained and/or wet perineal area,
convulsions, gasping, stained face, eyes half-dosed, and weight loss. All
deaths occurred within 1-6 days after dosing.
Finney, D. J., Problt Analysis, 3rd Ed., 1971, Cambridge University Press.
- 3-
n0t contain TSCAC
Sant*1-DeS
Comply
*** 95Z Confidence Liait: Males : Louer: Upper: Slope:
144 ng/kg 202 Bg/kg 8.9
Feules: Lower: Upper: Slope:
187 ng/kg 246 ng/kg 8.6
. Report by ,
John A. Hell Technician
Approved by:
Gerald L. Kennedy
Chief, Acute Investigations Section
JAH:jrg Date Issued: June 1, 1981
Report No. 291*81
- 4Company Sanitized, Does not contain TSCA CEJ
