Document 6w6Bj6eXpLLYxKxYNyRvNpkqd

ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted. The testsample isFC-201AF. Currentinformatioinndicatesitisa mixtureof3% PFOS, 18.59% diethyleneglycolbutylether,37.81% water,14% ethyleneglycol, 8.25% Acrylicfoanier,9% sodium oetylsulfate0,.3% sodium laurylsulfate, 3% triethanolamine6,% sulfonatedalkylethersalts,and 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely charactoilzeefconcentradons of impuddes. Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline202 Type: Acute static GLP: No Year completed: 1995 Species: Daphnia magna Supplier: In-housecultures.Originalloybtainedfrom U.S. EPA-ERL, Duluth, MN. Analytical monitoring: Temperature, pH, and dissolvedoxygen. Exposure period: 48-hours Test organism age: <24 hours old Statisticalmethods: Not applicabledue to study results. Test conditions: Dilutionwater: Carbon-filteredwellwater (3M Well #2, St.Paul, MN). Dilutionwater chemistry: Not given. Lighfing: Not given. Stock and testsolution preparation: A primary stock solutionwas prepared by dissolving1000 rngoftestsubstance in1000 mL water. Exposure concentrationswere prepared by additionofstocksolutiontoeach vessel. Exposure vessels: 100 mL vessels containing50 mL exposure solution. Number of replicates:4 Number of daphnids per replicate:5 Number of concentrations: one plusa blank control Water chemistry during the study: pH range (0-48 hours): 8.0- 8.1 (controlexposure) 8.0- 8.1(1000 mg/L exposure) Temperature range (0-48houm): 20.2- 20.4 OC Dissolved oxygen range (0-48houm): 8.3- 8.4mg/L (controelxposure) 6.9- 8.4 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank controland 1000 mg/L. Elementvalues: 48-hourEC5o=>1000mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplyto thatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance 48-hour EC50 was determined tobe > 1000 mg/L, the highestconcentrationtested.No mortalitwyas observed. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 2.This studymeets the criterifaorquality testing.However, sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmationofthe amount offluorochemicalproportionin solution. REFERENCES This study was conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number N2964, 1995. OTHER Last changed: 6/26/00. ENVIRONMENTAL LABORATORY LR N29r>4 48-HR ACUTE TOXICITY TO DAPHNIA, DAPHNIA At4GNA TYPE TEST: STATIC X STATIC RENEWAL TEST SUBSTANCE: 1-13652(FC-201 AF) Amber brown liquki-MiscibW whh water. DILUTION WATER: TEST DAPHNID SOURCE: CARBON-FILTERED WELL WATER (3M WELL #2. ST. PAUL, MN) TEMP. 20.4 'C, pH 8@O D@0. B-4 rrw;,t USEPA-ERL, Duluth, MN DATE RECEIVED: 6128,11990 DAPHNID AGE: <24 HR OLD NEONATES NO. OF DAPHNIDS,TEST CH"BER: 5 LOADING RATE: 5 daohnids;50 m.L TEST SOLUTION VOLUME: .50 n* SIZE OF TEST VESSELS: 100 mL- DATE INITIATED:- DATE TERMINATED:, TEST VESSELI BK1 BK2 BK3 BK4 'TAL 'NC0 tNC. mg!L CONTROL CONTROL CONTROL CONTROL TOTAL AMOUNT ADDED* L40RTALfTY 24-HR 0 0 0 C) 0 0 0 0 -'L---''pH D-0. mgil 1 1000 50 0 0 2 low 50 0 3 low 50 0 4 low 50 TEMP. *C DATE 1* ANALYST zo. z- 9/:Sf ECSO (95% C: rng -,COMMENTS *niL.Stock solution1000 rng-compouhd 1000 mL Carbon-fifteredwell water. lnftipaHl of highestconcentrationtested 1000 rrKyL is8.3.