Document 6w161Zqxxme4qeO8Rjm0dm5p9
AR226-2783
FOR DU FONT USE ONLY
Copies to:
Haskell
E. I. du Font de Neeours and Co., Inc.
Laboratorylfor Toxicology and Industrial Elljctontoad, P. 0. Box 50,
He wirk., Delaware 19711
Medicine
HASKELL LABORATORRYEPORT NO. 537-82
Material Tested*
Octaaolc aeid, peneadecafluoro-,
aambnium sail:
Basket! fe."
12.037
Other Cooes" C=8----------
(13,584
Study Initiated/Completed 6/2/82-6/16/82
Material Submitted bl
epartment
Heskell Laboratory
FOURTEEN-DAY FEEDING STUDY IN MALE AND FEMALE MICE
--------------------I------------------------------------------------
I ^
^;CI^-1 aale and ifeasie alee were fed eitheijUBH _
U|at lum
total of All aale
dose levels of either 3000, 300, 30, Ivor 3 ppm, or
perrxluuoorroooocCcaannooaacte aa|lct 3300 ppa or the 2 tect materials combined 30 ppm, (15 ppo each or 25 and 5 ppo each) for 14 consecutive and fema1 ntee died| within 12 days after feeding
3000 and 300 ppo.
for a days.
- " a t Uver Height/body Height-ratios -howed an increase at all levels tested
when compared to the controls. Mixing the 2 test eateriale asd feediag at 30 ppa did not alter the effects,, The amaatteerriiaals appear to have similar
^ f S I ^ ^ ^ -- 1 9 ^ potentials for causing l,iivgeejr^_nelnAlaErggfeiBBefiant--^^in-^niiccee^* _LLl.iyeir enlargement appears
to be dose- related Hitn(^^^^^^^^^^^^U|U|^eing the more toxic of
the two aaterials tested)
Purpose; To determine:
1
At what dose levels liver enlargement is produced.
The effects of mixing with amncniua perfluorooetanoate. Comparison of the ability of the 2 test materials to produce
liver enlargement;.
no^on^'s" TSCACBl
San^'.aed.^395 CoWpa^
IIHHUHIBUUil^ Procedure; fifty-five oale and 55 female Cri:CI>-l mice (age 44 day,
weighing 24-35 g) were grouper loto 22 group*, 5 mice per sex per ^roup, by cofflDuterizad^andoBization.-The mice uera homed individually and fed
dietary levl of either 3000, 300, 30, 10 or 3^p^orai3onn^perfiruoro<rctanoate afc 30 ppniior mixtures of the 2 materials for 14 consecutive day Two I groups of oale and 2 groups of female controls were observed concurrently aa^ fed only ground Purina Lab CboW. Individual body ueigbts were recorded at;0, 7, 9 and 14 days, clinical signs read daily and liver Heights recorded at the 14-day sacrifice.
Besulea: Male Mice
Dose Level
Ppn
0 3 10
30 300 3000
B-13.584)
Average Body Nblghts
After Feedio
TTBSr 2 Use
32
35
34
36
33
29
33
36
20
**
18
**
^tet^ Average Liver
2.08 3.01 4.01 3.85
Average Liver ffeighe/
Body ffeighfc Bacio
(g/lOOg Body Htight)
6.01 8.44 13.68 10.46
Amnoniun perfluorooctanoate (B-12,037)
Dose Level
Ppa
30
Average Body HSsighca
After Feeding'(g) 1 Neeic 2 leeks
31
33
Average Liver
^t (g>
4.54
Average Liver Height/ Body Hfight Ratio
(g/lOOg Body ifeight)
13.68
Dose Level
Pf
15/15 5/25 25/5
ionium lyerfluorooctanoate (B-13,584/H-12,037)
Average Body (fcftghts
After Feeding .(g)
1 Heete
Keeks
Average Liver
W^ght <^
Average Liver Height/ Body Height Ratio
(g/lOOg Body Height)
33
33
31
34
33
34
4.50 4.43 4.83
13.43 13.08 14.12
G&^p2atnv Ss"
Results; Female Mice
Dose Level
ppm
0 3 10
30 300 3000
Average Body tfeights
After Feeding (g)
1 Week 2 teeks
26
2^
27
30
26
26
27
29
15
**
16
**
Average Liver ttlght (g)
1.61 2.2? 3.41 3.08
Average Liver Ifcight/ Body ffeigbt Betio
(g/lOOg Body Ifeleht)
5.71 7.67 13.86 10.47
AmaonluB perfluorooctanoate (H-12,037)
Doae Level P1"
,,, ,,
30
[
Dose Level
PPtt
15/15 5/25 25/5
Average Body Heights
After Feeding (g) 1 Keek 2 UecL
Average Uver (tight (g)
Average Uver Ifcight/
Body ffelght Ratio (g/lOOg Body ffeiKht)
26
27
3.65
13.29
IBilHBH^sEl!TM perfluoroocCanoate (H-13.584/B-12.037)
Average Body I^ighca After Feeding (g)
1 Neck 2'ifc^r-~
Average Liver Ifcight (g)
Average Liver (bight/
Body Neight Ratio (g/lOOe Body tfciznt)
27
28'
26
28!
26
28!
3.81 3.79 4.13
13.61 13.53 14.53
** All animals died at this dose level.
* H-12,037
\ Contaminants: ^^H^HH^BI
Synonyms: o ^BBOniu^^rfluanao^anoaee.
S ^^M^^B^^--^^^^^^"--^--------!
----^
- 3
* H-13,584
^ Purity:
CAS Registry Number: r^|
Beport by:
^^ / ^.
John E,. HBeennryrv
Technician
!Reviewed by:
luls Daahlell Study Director
\.. Approved by:
.r<-, f'o/* S'
%aid L. Kennedy,^.----------
^xs^.-L
J
Section Supervisor
Acute Investigations
JEH:tac;WP;4.10
-
4 -
1SCACS
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