Document 6bzD5Q3abLo6BeXERK3aDwv3R

BACK TO MAIN U .Ilf .... TECHNICAL REPORT SUMMARY 11/ 19/80 TO: TECHNICAL COMMUNICATIONS CENTER - 201-2CN (Importuni If report is pruned on both sides o f paper, send two copies to TCC.) D iv isio n Environmental Laboratorv ProKH : l rnmmprr'.inl Chemicals R e p o rt T itle (EE & PC). Aciuatic Toxicitv Testing: CS^2151. To D e p t . Number St. Paul. Minnesota 0535 P rolact N um bar ,, 9970012600 Raport Num ber Lot 3 (LR 6260S)__________ 046 E. A. Reiner A u th o r(s) . M. T. F.lnabarawv - 2-3E N o te b o o k R e fu ro n ce ' ..... SECURITY C Open (Company Confidential) D Closed (Special Authorization) 3M C H EM IC A L w, REGISTRY w Em ploye Num ber(s) 46981 N o. of Pagas Including Covershaat 4 New Chemicals Reported O Yes H No KEYWORDS: (Select terms from 3M Thesaurus. Suggest other applicable terms.) EE & PC - Div. (Env. Lab) CURRENT OBJECTIVE: To determine the acute aquatic LC*q following exposure to varying concentrations of CS-2I51, Lot 3. Toxicity (Aquatic) _______________________________________________________________ R E P O R T A B ST R A C T: (200-250 words) This abstract information is distributed by the Technical Communications Center tc alert 3M'ers to Company R&D. It is Company confidential material. The acute static aquatic 96-hour LC-q values obtained for fathead minnows (Pimephales promelas) following exposure to CS-2151 in replicated experiments were 199 (172-231) and 153 (126-181) mg/1. The combined 96-hour LCgQ was 175 (156-195) mg/1. Data generated include LC^q values with 95% confidence limits. Information Liaison Initials: BACK TO MAIN CONCLUSIONS In these replicated 96-hour LC50 aquatic tests, the calculated values for fathead minnows (Pimephales promelas) were 199 and 153 mg/1. Materials possessing an LC50 of this order of magnitude are considered to be practically nontoxic based on the NIOSH criteria for aquatic toxicity interpretation. (2) INTRODUCTION: The test substance is a light yellow liquid, soluble in water at ambient room temperature. Tests performed on this material evaluated its potential aquatic toxicity and the data generated form the basis of this report. ACCLIMATION AND EXPERIMENTAL DESIGN: The test protocol utilized for this study was modeled after that described by ASTMrE729-80. (1) The fathead minnows (Pimephales promelas) were obtained from a private hatchery (a). Stock fish were held in a flow through system fiberglass holding tank filled with carbon-filtered wellwater at 14-16 degrees C. A daily photoperiod of 16 hours light and 8 hours dark, with a 30-minute transition period was maintained throughout the acclimation and testing period. The fish were fed Tetra-Min (b) daily, food being withheld 48 hours prior to and throughout the test period. Pish were acclimated to test temperature for 2 days prior to use in assays. Twenty (20) fathead minnows, uniform in size, were tested at each chemical. All-glass aquaria, 35x20x25 cm containing 16 liters of diluent plus toxicant comprised a study chamber. Test fish were randomly allocated to each test chamber within 30 minutes following toxicant addition. The fathead minnow experiments were conducted at 19+2 degrees C. Mortality, temperature, dissolved oxygen level, and pH of all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations were recorded relative to swimming pattern and behavioral changes induced by exposure to the test chemical. The acute short-term staic bioassays were performed on CS-2151 using carbon-filtered well water of known composition and constant quality as the diluent (Tables 1,2). a) Dale Fattig Fish Farm, Brady, Nebraska. b) Commercial Fish Food of known composition. BACK TO MAIN The 96-hour fish studies were performed in replicate using the following CS-2151 logarithmic concentrations: 87, 135, 210 320, and 490 mg/1. The range of dilutions was selected to bracket 96-hr. LC50 values predicted from preliminary exploratory toxicity tests. LC50 values with 95% confidence limits were calculated using the probit program and 3M TRAC Computer system. Monitoring equipment used included ASTM thermometers, Orion temperature-- compensating pH meter, a Yellow Springs dissolved oxygen meter, and a Taylor temperature chart recorder. RESULTS: The fathead minnows (Pimephales promelas) used in these studies had an average weight of 0.3 grams and a length of 3.5 cm. The loading factor was 0.4 g/1; the maximum recommended loading factor is 0.8 g/1 (1). Temperature, pH, and dissolved oxygen values and ranges obtained during this study were: 19+ 2 C., 7.6 - 8.0 pH units, and 96-hour dissolved oxygen values ranged from 5.2 - 5.9 mg/1. These latter values calculated out to a minimal oxygen saturation of 56-63%. In this replicated study, the fish 96-hour LC50 values were 199 and 153 mg/1. The 1-, 2-, 3-, and 4-day LC50 values and their 95% confidence limits and the method used to calculate them are listed in Table 3. Chemicals possessing an LC50 value in this range are considered to be practically nontoxic to the aquatic environment (2). The data reported herein are based on studies performed by M.T. Elnabarawy and R. R. Robideau. Accompanying data sheets comprise original data. A copy of this report is filed in the Environmental Laboratory Archive System, Building 2-3E. It should be noted that the generated results were reported in the absence of information relative to the "purity" and the "stability" of the chemical substance CS-2151, Lot 3. The reported LC50 values were calculated on the basis of the "observed mortality rates" and of the calculated initial concentrations of CS-2151 in test solutions. REFERENCES: (1) ASTM-Standard Practice E729-80. Standard Practice for Conducting Acute Toxicity Tests with Fishes, Macro invertebrates, and amphibians. American Society for Testing and Materials, Philadelphia, PA 1980. (2) Registry of Toxic Effects of Chemical Substances, '76 Edition, Christensen, H. E., and E. J. Fairchild, Eds., p. XV and Ci, U.S. Department of Health, Education and Welfare; National Institute for Occupational Safety and Health, Washington, D.C. (June, 1976). BACK TO MAIN TABLE 1 WELL WATER QUALITY SAMPLE DATE: 9/30/80 SAMPLE TAKEN: 3M Bldg. 2-3E, St. Paul SAMPLE DATE 9/30/80 (TAKEN FROM TANK *4) PARAMETER CONCENTRATION ENV. LAB WATER QUALITY BOOK REF. PH Residual Chlorine Ammonia Nitrogen Conductivity M-Alkalinity Total Solids Total Hardness Total Fluoride Dilute COD Total Coliform Standard Plate Count 7.7 <0.02 mg/1 <0.1 mg/1 as N 380 umhos/cm at 25C 230 mg/1 as CaC3 ' 324 mg/1 244 mg/1 as CaCO <0.02 mg/1 <0.2 mg/1 Negative 108 per ml 8 9 8 9 8 8 8 9 9 10 10 Aluminum as Al* 0.01 mg/1 Arsenic as As* <0.005 mg/1 Boron as B* <0.2 mg/1 Cobalt as Co* <0.002 mg/1 Copper as Cu* 0.018 mg/1 Chromium as Cr* <0.005 mg/1 Cadmium as Cd* <0.002 mg/1 Iron as Fe* 0.022 mg/1 Nickel as Ni* 0.005 mg/1 Lead as Pb* . <0.005 mg/1 Silver as Ag* <0.002 mg/1 Zinc as Zn* 0.13 mg/1 Mercury as Hg* <0.2 ug/1 14 14 14 14 14 14 14 14 14 14 14 14 14 PCB's** <0.50 ug/1 36 Total Organochlorine** <0.50 ug/1 Pesticides 36 Total Organophosphorus** Pesticides <0.50 ug/1 36 * Sample Date 3/31/80 **Sample Date 2/26/80 BACK TO MAIN TABLE 2 ENVIRONMENTAL LABORATORY ,BLDG. 2-3E ST. PAUL MINNESOTA THIRD QUARTERLY REPORT, 1980 WELL WATER SUPPLY FOR AQUATIC TOXICITY TESTING PARAMETER TOTAL HARDNESS* as mg/1 CaCOj AVERAGE JULY 31 AUG. 29 SEPT. 30 ( S) 256 244 252 251 + 6 TOTAL "M" ALKALINITY* as mg/1 CaCO 221 219 223 221 + 2 ELECTRICAL SPECIFIC 380 390 360 377 + 5 CONDUCTANCE* as umhos per cm2 at 25C TOTAL SOLIDS* as mg/1 368 404 350 374 + 27 pH** 7.6 7.6 7.6 7.6 + 0 STANDARD PLATE COUNT*** per 1.0 ml 119 94 Lab 107 + 18 Accident CRITERIA OF CONSTANT QUALITY * Monthly ranges should be less than 10% of their respective averages. (ASTM Standard E729-1980.) ** Monthly range of pH should be less than 0.4 unit. (ASTM Standard E729-1980.) *** Total bacterial densities should not be greater than 500 organisms per ml. (USEPA-600/8-78-017, p. 101.)