Document 6bnEr63ZnV5vaqnr8d3a9KVbm

A C U T E T O X IC IT Y T O F ISH (FA T H EA D M IN N O W ) T E S T S U B S T A N C E __________________________________________________ Identity: Mixture containing perfluorooctanesulfonate which m ay also be referred to a s P F O S , FC-95, or a s a component of FC-3003. (1Octanesulfonic acid) (C A S # 2795-39-3). R e m arks: The 3M production lot number w as not given. The test sam ple is FC-3003. Current information indicates it is a mixture of 1 % P F O S , 1 5 % diethylene glycol butyl ether, 7 9 .3 9 % water, 2 % Sultone foamer, 1 .5 % sodium octyl sulfate, 1 % sodium decyl sulfate, 0 .0 6 % sodium lauryl sulfate, and 0 .0 5 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity o f the fluorochemical component o f the test sample. M E T H O D :___________________________________________________________ M ethod: O E C D Guideline. Type: Acute static range-finding study GLP: No Y e a r com pleted: 1997 S p e c ie s: Pimephales promelas Su p p lie r: Aquatic Biosystem s, Inc., Fort Collins, C O A nalytical m onitoring: Temperature, pH, and DO. E x p o su re period: 96-hours Sta tistical m ethods: Not given. T e st fish age: Juveniles (128-132 days) Le n gth and w eight: A verage length = 23 mm Average weight = 0.09786 g (wet) L o a d in g: 0.2936 g/L Pretreatment: None T e st conditions: D ilution water: Carbon-filtered well water (3M W eil #2, St. Paul, M N). D ilution w ater chem istry: Not given. Ligh tin g: Not given. S to c k and te st so lu tio n preparation: The test material w as added directly to the dilution water on a weight/volume basis. C once ntration s d o sin g rate: Once. Sta b ility o f the te st chem ical so lu tio n s: Not noted. E x p o su re v e sse ls: 1.2 L v e sse ls containing 1.0 L test solution. N um b er o f replicates: One, Bk control duplicated N u m b e r o f fish p er replicate: 3. N u m b e r o f co n ce n tra tio n s: three plus 2 blank controls W ater chem istry d uring the study: 002732 pH range (0-96 hours): 7.9 - 8.2 (control exposure) 7.7 - 7.9 (1000 mg/L exposure) Tem perature range (0-96 hours): 18.5 C D isso lve d oxygen range (0-96 hours): 7.8 - 8.5 mg/L (control exposure) 4.5 - 8.6 mg/L (1000 mg/L exposure) R E S U L T S ________________________________________________________ ___ N om inal c o n ce n tra tio n s: Blank control, 10,100, and 1000 mg/L Elem ent v a lu e s: 96-hour L C 50 = <1000 mg/L but >100 mg/L Elem ent values based on nominal concentrations R e m a rk s: Testing w as conducted on the mixture a s described in the Test Sub stan ce Rem arks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ______________________________________________________ T he test sam ple 96-hour L C 50for fathead minnow w as determined to be <1000 mg/L. The 1000 mg/L exposure caused 6 6 % mortality while the 100 mg/L exposure had no mortality. Subm itter: 3M Com pany, Environm ental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 D A T A Q U A L IT Y ________ ___________________________________________ Reliability: Klim isch ranking = 3. The study is a range-finding, with limited exposure concentrations and test organism s. Sam ple purity w as not properly characterized and the study lacks analytical confirmation of the amount of fluorochem ical proportion in the solution. R E F E R E N C E S _______________________________________________________ T h is study w as conducted by the 3M Com pany, Environmental Laboratory, St. Paul, MN, Lab Request number R 1 3 3 1 ,1997. O T H E R _________________________________________________________ ____ L a st ch a n ge d : 6/27/00 002733 ENVIRONMENTAL LABORATORY 98-HR ACUTE TOXICITY TO FATHEAD MINNOW, PIMEPHALES PROUELAS LR i Pfl-- I___ L ^ . TYPE TEST: 8TAT1C X STATIC RENEW AL ______ TEST SUBSTANCE: DILUTION WATER: Fc. - A F F F L A u.<>4-OqP I q ) .CAHBON-HLTERED W ELL WATER O M W ELL 92 8T. PAUL. MNI Tm p.______ *C ,pH ______ , D .O .______ m g/L TEST FISH SOURCE: fivo 5 j S \ e v n S DATE RECEIVED: 2 - l %- S 7 HSHAGE: I 3. S' ' 13 X AVG. WT.:0.Ocn & , O O V49 g AVG. SIZE: 3 ?> 0 3 cm NO. OF F IS H IE S T CHAMBER: 3 ____________ LOADING RATE: Q , 2.9 5 6 TEST SOLUTION VOLUME: ) ,Q C _________ SIZE OF TEST VESSELS: I . 3. L DATE INITIATED: M - 1M - ^ "1 DATE TERMINATED: W \ 2 ' ^ 1 1130 TEST INITIAL VESSEL! CONC. 1 24-HR PH O ' 7 ` O 0.0. 48-HR mg/L 1-S o o MORTALITY t I PH D.O. 72-HR^ pH ^ D.O. wO*frri pH | D.O. mg/L mg/L *.c> ^ j i i B, o io V 5 H ,5.\ A S ' ! mg/L o , *1 * 1V.T-i ^ > 4 TEMP. C 1 8 .5 DATE / ANALYST M -lS -*n H o o S k j s i tt OlArr /8 5 cc . iS 'p m M- It-S T T/Ls l h ! < n Afe 4 - IS - c , ! TVc LC50 (95% C.l.) <<-M Q g < I O O P p-vj \ L. V ^ O-N ___S s m C7 l\ ri\iSol<_i e_d C P o k d ilt. \ r \ 'J Loa-Ae.r t o A V\ tsV iP rJio& ,, C lo n e . 4 'r i l o o o v n v . \ L Y CO.. -e o o i /-/fc -tf i ^ ~Lo_ O O -Pcx\o v*'G SECONDARY EFFECTS: S SurMt ; E L o u ol Equifcflum; Q * QulMCtnt; R Erraic Swimming; H * Hwnarthaglng ANALYST: "T*<S BEST COPY AVAILABLE 002734 Environmental Laboratory LR: C l 1 3 a I Date: f - 'l r - q i In itia ls: Page of 2 96-Hour Acuta Toxicity To Fathead Minnow, Plmaphalee Pronwla* Loading Rate Data Sheet Teel Substance: P c - 5 o o 3 f \ F f T C A u S T t U U - I A 3 _______________ Rah * 1 2 3 4 5 6 7 8 9 10 Average Weight, (g) o ot O .ojxi 0-12-33 a- ii 0 -0 ? 11 O. o 9 H x 0.066, / O. 0 6 33 0 . 2 O f 1 o. Lenath. (cm) l? as <3* 2a os 2-X 2.S 2.X d3.G, Loading Rate Calculation : Avg. wt. (g.): O- O 9~I Pfe Std. Dev: O O ^6^ No. fish/vessel`___ 3 ________ VoL/veasel: * _______ O- 3,9 35jpq. fish * L Loading rate: O ' 3 5 Co 002735