Document 6bOgQqqzbaDGq01bvvXdY6w1E

AR 556 _Ol&lj- ACUTE TOXICITY TO INVERTEBRATES (GRASS SHRIMP) TEST SUBSTANCE____________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-206, identified by the laboratory as "Sample C". Current information indicates it is a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, and 0.05% benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD:____________________________________________________ Method: Standard Methods (APHA) 1971 Edition Type: Acute static GLP: No Year completed: 1974 Species: Palaemonetes vulgaris Supplier: Field collected locally in MA. Analytical monitoring: Temperature, and dissolved oxygen. Exposure period: 96-hours Statistical methods: TL50 (median tolerance limit) values calculated using a linear regression equation. Test organism age: Juveniles. Length and weight: Average length = 30 mm Loading: Not given. Pretreatment: None Test conditions: Dilution water: Synthetic sea water Dilution water chemistry: pH 8.0 Salinity 25 ppt Lighting: Not given. Stock and test solution preparation: Not given. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 5 gallon glass vessels containing 15 L of test solution. Number of replicates: 1 00147 Number of organisms per replicate: 10 Number of concentrations: six plus a blank control Water chemistry during the study: Temperature range (0-96 hours): 20 1 C Dissolved oxygen range (0-96 hours): 4.2 - 8.6 mg/L RESULTS____________________________________________________ Nominal concentrations: Blank control, 100,180, 240, 320, 420, and 1000 mg/L Element values: 96-hour TL50 = 280 (230 - 342) mg/L 96-hour NOEL = 180 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test sample 96-hour LC50 for grass shrimp was determined to be 280 mg/L with a 95% confidence interval of 230 to 342 mg/L. The 96-hour no observed effect level (NOEL) was 180 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY _____________________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES________________________________________________ Test was conducted by Bionomics, EG&G, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1974. OTHER______________________________________________________ Last changed: 6/28/00 0014S0 ccl-e -- XDa ^ -! ^ C ^-v-dU ^o 9 / C fc-O Vs o o C r & k 0LC BIOASSAY REPORT SUBMITTED TO 3 .M COMPANY SAINT PAUL, MINNESOTA ^ I ACUTE TOXICTY OF SAMPLE C TO MUMMICHOG (Fundulus heteroclitus) GRASS SHRIMP (Palaemonetes vulgaris) , AND FIDDLER CRAB (Uca pugilator) . Bionomics E G & G , Inc. 790 Main Street Wareham, Massachusetts May, 1974 0014S1 METHODS AND MATERIALS These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of mummichog (Fundulus heteroclitus) , grass shrimp (Palaemonetes vulgaris) , and fiddler crab (Uca pugilator) to Sample C, a brown liquid, tested as 1007 active, was evaluated under static bioassay conditions for a 96 hour period. The results were reported as the medi&n tolerance limit (TL^q ) , the concentration of the sample in . water causing 50 percent mortality after 24 and 96 hours. The predicted TL^q value and its 95% confidence intervals were arrived at by converting the concentrations tested and the corresponding observed percent mortalities, to logs and probits, respectively. These values were then used to calculate a linear regression equation. Test procedures for the static bioassays are those described for Fish Bioassay Procedures in the 1971 edition of Standard Methods (APHA). The mummichog, grass shrimp, and fiddler crabs used in these tests were collected locally by laboratory personnel. The mummichog had a mean weight and length of 1.1 g and 35 mm,respectively. The grass shrimp had a mean length of 30 mm. The fiddler crabs had a me width of 20 mm. 001482 Page two The test species were observed in the laboratory hatchery facility for at least 30 days prior to testing. These species were acclimated over a 10 day period.prior to testing,to laboratory test diluent water quality and temperature. During that period mortality in the test population was^37o and these animals were judged to be in excellent physical condition. The bioassays were conducted in 5 gallon glass vessels containing 15 1 of water at 20C (-1.0), and there was a single introduction of the sample into each test vessel. The test containers were not aerated. The test diluent consisted of synthetic sea water`d with a salinity of 25 /oo, a pH of 8,0 and a temperature of 20C 1.0). Dissolved oxygen levels for the tests ranged from 8-6 at the beginning of the tests to 4.2 at the end of testing. Ten specimens were introduced into each test vessel 24 hours prior to the start of the assay. RESULTS The predicted TL^q values and 957 confidence intervals are presented in Table 1. Table 2 presents a summary ^Laroche, G. E. Eisler, and C. R. Tarzwell, 1970. Bioassay Procedures for Oil and Oil Dispersant Toxicity Evaluations. J. W. Poll. Cont. Fed. 42 (11): 1982-1989. 001483 Page three of observed mortality for each individual concentration after 24 and 96 hours of exposure. The mortality syndrome in all tests was similar. The mummichog and grass shrimp generally became dark and lethargic, lost equilibrium, and expired. The fiddler crabs became lethargic before expiring. 001484 SUBMITTED BY: PREPARED BY: Bionomics E G & G, Inc. 790 Main Street Wareham, Massachusetts May, 1974 ^ Robert E. Bentley APPROVED BY: Bevier Hasbrouck Sleight, III Director, Freshwater Programs 0014S5 Table 1 -- Acute toxicity of Sample C to mummichog3 (Fundulus heteroclitus) , grass shrimp*5 (Palaemonetes vulgaris'), ai fiddler crab (Uca pugilator) . These data are based on results of bioassays conducted at the fish toxicology of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species mummichog C.o a jtv | a grass shrimp fiddler crab TL^q - milligram/liter 24 hour 96 hour No Effect Level (mg/1) >1,800 <2,400 1,820(1,360-2,420)d 1,400 >1,000.0 (^280 (230-342) 180 >10,000 3,260(2,760-3,860) 2,400 aAssays conducted at 20C (- 1.0), mean weight of the mummichog 1/ ^Assays conducted at 20C (* 1.0), mean length of the grass shrimp 30 mm. cAssays conducted at 20C (* 1.0) , mean carapace width'of the fidd ..' - -- crabs 20 mm. d957o confidence intervals . - -- CaJQ/a! J 001486 Table 2 -- Concentrations tested and corresponding observed percent mortalities of mummichog (Fundulus heteroclitus) , grass shrimp (Palaemonetes vulgaris) , and fiddler crab (Uca pugilator) after 24 and 96 hours of exposure to Sample C. Species mummichog Concentration (mg/1) 3,200 2,400 1,800 1,400 1,000 control % mortality observed 24 hour 96 hour 100 100 0 0 0 0 100 100 30 0 0 0 grass shrimp 1,000 420 320 240 180 140 control 0 100 0 100 0 100 o 40 0 20 ~ 00 00 001487 Table 2 Continued Species fiddler crab Concentration (mg/1) % mortality observed 24 hour 96 hou 10,000 6,500 4,900 3,700 2,800 2,400 2,100 control 100 0 0 0 0 0 0 0 100 100 100 70 30 0 0 0 0014S8