Document 6bDmn3OKRjvDB2bEQBeEayL5R

M76-OOSX Be-) ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic add, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The test substance is a white powder. Sample was taken from 3M lot number 217. Sample was stored under ambient conditions prior to testing. Purity determined to be 90.49% by LC/MS, 1H-HMR, 19F-NMR and elemental analyses techniques. METHOD Method: OECD 203 and OPPTS 850.1075 Type: Static acute GLP: Yes Year completed: Study completed 1999. Report completed 2000 Species: Pimephales promelas Supplier: In-house cultures, Wildlife International, Ltd., Easton, MD Analytical monitoring: PFOS measured at 0, 48, 96-hours Exposure period: 96-hours Statistical methods: LC50 values calculated, when possible, by probit analysis, moving average method or binomial probability with non-linear interpolation using the computer software of C.E. Stephan. Test fish age: Approximately 126 days old Length and weight: 35 (30-38) mm, 0.36 (0.21-0.49) g Loading: 0.24 g fish/L Pretreatment: None Test conditions: Dilution water: 0.45 pm filtered well water Dilution water chemistry (during the 4-week period immediately preceding the test): hardness: 131 (128-136) mg/L as CaCC>3 alkalinity: 177 (176-178) mg/L as CaC03 pH: 8.3 TOC: <1.0 mg/L Conductivity: 311 (310-315) pmhos/cm Stock and test solution preparation: Primary stock prepared in dilution water at 27 mg/L and mixed for ~22 hours prior to use. 000539 After mixing, primary stock solution was proportionally diluted with dilution water to prepare the four additional test concentrations. Concentrations dosing rate: Once Stability of the test chemical solutions: Extremely stable Exposure vessels: 25L polyethylene aquaria containing approximately 15L of test solution; water depth approximately 17.6 cm. Number of replicates: two Number offish per replicate: ten Number of concentrations: five plus a negative control Water chemistry during the study: Dissolved oxygen range (0 - 96 hours): 7.8 - 8.8 mg/L (control exposure) 7.7 - 9.0 mg/L (28 mg/L exposure) pH range (0 - 96 hours) 8.3 - 8.6 (control exposure) 8.4 - 8.5 (28 mg/L exposure) Test temperature range (0 - 96 hours) 20.4 - 22.1 C (control exposure) 21.3 - 22.3C (28 mg/L exposure) - Method of calculating mean measured concentrations: arithmetic mean RESULTS Nominal concentrations: Bk control, 3.6, 5.9, 9.9, 16, 27 mg/L Measured concentrations: <LOQ, 3.3, 5.6, 9.5, 17, 28 mg/L Element value: 24-hour LC50 = > 28 mg/L (C.l. not calculable) 48-hour LC50 = > 28 mg/L (C.l. not calculable) 72-hour LC50 = 27 (22 - 41) mg/L 96-hour LC50 = 9-5 (8.0 -1 1 ) mg/L All element values based on mean measured concentrations Statistical Evaluation of Mortality: Confidence limits for 24 and 48-hours could not be calculated due to lack of mortality. The 72-hour LC50 value is questionable because a concentration-effect relationship was not demonstrated over a reasonable range of percent dead. The 24 and 48-hour LC50 values were determined by visual interpretation. Probit was used to calculate the 72-hour LC50 and Moving Average for the 96-hour LCso- Analytical Methodology: Analyses of test solutions were performed at Wildlife International Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the 000540 concentration of the test substance in the test solutions, the same and most prominent peak response for perfluorooctanesulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The LOQ (limit of quantitation) was 0.458 mg/L in this study. The mean percent recovery of matrix fortifications analyzed concurrently during sample analysis was 97.9. Samples collected at test initiation had measured values from 85.3 to 117% of nominal. Measured values for samples taken at 48-hours ranged from 86.3 to 101% of nominal. Measured values for samples taken at 96-hours ranged from 87.6 to 98.3% of nominal. Summary of analytical chemistry data: N om inal T est C o n c e n tra tio n , m g/L N eg ativ e Control M easu red D uplicate V a lu e s at 0, 48, and 96-hours, Respectively, m g/L M ean M easu red C o n c e n tra tio n , m g/L All < L O Q <LOQ Percent of N om inal _ 3 .6 3.1 6, 3.53, 3.0 8, 3.22, 3.46, 3.1 3 3 .3 92 5 .9 6.0 5, 5.07, 5.48, 5.89, 5.70, 5.55 5 .6 95 9 .9 8 .9 9 , 9.47, 9.88, 9.33, 9.70, 9.5 2 9 .5 96 16 1 8 .2, 19.3, 15.0, 15.6, 14.8, 16.2 17 106 27 28 .5, 28.5, 27 .0, 27.8, 26.8, 26.6 28 104 Biological observations after 96-hours: Fish in the negative control and the 3.3 mg/L exposure concentration appeared normal. Some or all of the surviving fish were observed to be swimming erratically (4/16 in 5.6 mg/L exposure, 10/10 in 9.5 mg/L, 4/4 in 17 mg/L) at test termination. Cumulative percent mortality: M ean M easured T est C one., m g/L 2 4 -h o u rs N eg. Control 0 3.3 0 5.6 0 9.5 0 17 0 28 0 4 8 -h o u rs 0 0 0 0 0 0 7 2 -h o u rs 0 0 0 0 15 50 9 6 -h o u rs 0 0 20 50 80 100 000541 Lowest concentration causing 100% mortality: 28 mg/L Mortality of controls: None CONCLUSIONS The potassium perfluorooctanesulfonate 96-hour LC50 for fathead minnow was determined to be 9.5 mg/L with a 95% confidence interval of 8.0 -11 mg/L. The 96-hour no mortality and no effects concentration was 3.3 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking = 1 REFERENCES This study was conducted at Wildlife International Ltd., Easton, MD at the request of the 3M Company. OTHER Last changed: 5/3/00 000S42