Document 6b5JV1xp2np6ZBLgYExMeaEZ6

{AR 226-0538 MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorooctylsulfonate, didecyldimethylammonium salt; may also be referred to as Fluoroalkyl ammonium derivative. [1D1,e1c,a2m,i2n,3i,u3m4,,4N,-5d,e5c,y6l,-6N,,7N,-7d,i8,m8e,t8h-yhle-p,tasadletcawfiltuhoro-1- octanesulfonic acid (1:1), CAS # 251099-16-8] Remarks: The 3M production lot number was Lot 1. The test sample is LL-14394 referred to by the test laboratory as P3025. The sample was labeled F-11615, Lot1. The test sample is a mixtureof the test substance in water (approximately 30-40% test substance, 60-70% water, and 0-5% of residual perfluorochemicals). All values reported relate to this mixture. The test sample appears to be a 2-phase dispersion (clear liquid with opaque solid) which rapidly separates after agitation. No calculations `were made to adjust for the actual concentration of the test substance in the test sample. METHOD: Method: Azur Environmental Corp. Microtox "BASIC" Procedure Test type: Static acute GLP: No Year study completed: 1996 Year report completed: 1997 Species: Vibrio fischeri formerly Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition Replicates: Two Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Azur Microtox Reagent. Test Conditions: Dilution water: Azur Environmental Corp. diluent Stock and test solution preparation: Water accommodated fractions. Test solutions were prepared for each test concentration by mass addition of test substance in Millipore Mill-QTM water. The solutions were stirred overnight in the dark at ambient room temperature. A white precipitate was observed in the stock solutions after mixing. Prior to test initiation, a 10 mL aliquot of the clear liquid portion of each test solution was removed and each was osmotically adjusted. All test solutions were made by proportional dilution. Exposure vessels: 4 mL glass cuvettes. C4231 Number of replicates: Two Number of concentrations: Four plus a control. Element Basis: Percent light loss. RESULTS NEolemmiennatlvcaolnuec:entrat5i-omnisn:utBelaEnkCsco=nt6ro0l0, (15258,0-265200,) 5m0g0l,L1000 mg/L 15-minute ECso = 510 (500-530) mg/L 30-minute ECso = 350 (240-480) mg/L All element values based on nominal concentrations. Remarks: Values reported are for the test sample. No calculations were `made to adjust for the concentration of the test substance in the test `sample. CONCLUSIONS _ The test sample 30-minute ECs for Vibrio fischeri was determined to be 350 mg/L with a 95% Confidence Interval of 240-490 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY. Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. Additionally, data is foar mixture and toxicity cannot be positively attributed to didecyldimethylammonium Perfluorooctylsulfonate salt alone. REFERENCES TShtiPsauslt,udMyNw,aLsacboRndeuqcuteesdt bnyumtbheer3MP3C0o2m5pany Environmental Laboratory, OTHER Last changed: 5/24/00 C9432 IM ENVIRONMENTAL LABORATORY INHIBITORY EFFECTS OF P3025 ON MICROTOX `Azur Environmental Microtox System STUDY COMPLETED: December 19, 1996 FINAL REPORT COMPLETED: May 12, 1997 Env. Lab Request Number P3025 PNarmeep:ared by. Tite: Laboratory: Address: S`AudsvaanncAe.dBEenavcihronmental 3BiMoloEgnivsitronmental LBuaiblodriantgor2y-3E-09 9St35PaBulu,shMNAv5e5n14u4e. C0433 MPiacgreot1 ox May 12,1997 `The purpose of his study was to evaluate the inhibiSloUryMeMfAfeRctYof P3025 on bioluminescent bacteria in accordance wrietphorttheedAazsur3E5n0vimrgon.mental Corp. Microtox test. The median effeclive concentration, 30-minute ECs0 was `Toxicity tests with Microtox provide a rapid screIeNniTnRgOmDeUtChToIdOaNnd can be reliable predictors of the inhibitory effects of contaminants on microbial systems. 3"T0h-imsitnouxitceist)y sitnutdhyemperaessuernecde tohfedilifgehrteonuttpcuotncoefnbtiroaltuimoinsneosfcePn3t02b5a.cteria over a relatively short period (5, 15, and TestSubstance; Appearance Aqueous Solubility PrAovpaeilratbilees:Physicochemical Date Received Storage Test Solutions, Reported values MATEARNDIMEATHLODSS P3025, date 10/7/96, Lot 1 `Two-phase dispersion (clear liquid with opaque solid) `Apparenly not completely soluble in water at tested concentrations. VBoaiploirngPProeisnstu;re: VEavappoorraDteinosintyR:ate: Solubilty in water: SPpeercciefnitc GVroalvatiitlye: pH: c1a0.018CmmHg @ 20C <<11 cInaso1l2uble 66-08-70% 10/16/96 In the dark at ambient room temperature (22 + 29C) `sTteosctkssoolultuitoinosnswoefre25p0r,e5p0a0r,ed10a0s0W,aatnedr-2A0c0c0ommgm/oLdwaeterde Fprraecptairoensd (inWAMFiSl)l.porFeouMril Qruoaotmert.empEearacthuwrea.s cTeasptpesdubasntdanaclelowwaesd taoppmairxenotvleyrnniogthtc,oimnptlheetedlaryk,misatciabmlbeiweintth water. A few white particulates were observed in each stock solution after mixing. SJutsotckprsioolruttiootneswtaisnirtieatmioonv,ead 1a0ndmLeacahliwquaost oofsmtohteicclaelalry laidqjuuidstpeodrtwiiotnho2f0e0acmhg croeuargesnetogfrtahdeetNesatC.l. Each initial concentration was diluted by 50% curing the bTehgeinrneipnogrtoefdtvhaelumeicsroabriealbaassesady.on nominal concentrations in the test media at the CO4:34 MPiacgreot2ox May 12, 1997 Controls: Blank control Exposure; Test Date Contact Time. Vessels Number of Repiicates. Test Solution Vol, Starting TTeesstt Conc. Substance Dilution Water Temperature Lighting `nTowotecsotnsturbolsstacnocnet.aining Microtox reagent (Photobacterium phosphoreum), but 12/19/97 5, 15, and 30-minutes 4 mL Azur Environmental glass cuvettes Two 10m 125, 250, 500, and 1000 mg/L Azur Environmental Diluent (ultra pure water osmotically adjusted to 2% NaCl ) Instrument temperature set at 15C Ambient laboratory light Measurements. Procedure Laingdhtthreena'd,in1g5s,waenrde3t0a-kmeinnuutpeosnarfetecrontsetsittustuibosntoafnctehewMaiscraodldoexdtboitohleumbiacnteesrciean.t Ablalcteria, rienasdtirnugmesnwt,ere caplured electronically via computer interface with the Microtox The test was Basic Test. conducted in general accordance with the Azur Environmental Microlox CALCULATIONS `cTohnecernetproarttieodnsvatleusetsedaraendbatsheedcoornrneosmpionnadlincgonrceesnptornasteiodnastian dtehreivteesdt fmreodmitahaettotxhiecibteygtiensntianrgeoufstehde tteostc.alcTuhleate, if possible, the medial regression methods effective provided cionntcheentArzautrioEnn,vEirConsmeanntdalthseof9tw5a%rec.oTnfhiedeEnCcesinitserdveaflinbeyd satsantdhaercdonlocgeinsttircation eofsteixmpaotseudrteo {i0nhtibhiet ftehset rsautbesotfanbcieo.luminescence by 50% as comparetod the controls, after 5, 15, and 30-minutes C0285 MPiacgreol3ox May 12, 1997 RESULTS "mTihneutpeersceenxtproesduucrtteoiotnhientiegshttsouubtsptuatncaendP3th0e25meadrieasnuemfmfaerctiizveedcoinncTeanbtlraeti1.on, ECso. obtainedafter 5, 15 and 30T35h0e (m2e4d0i-an49e0f)femctoilve.concentration, 30-minute ECS and 95% confidence interval was reported as ACCEPTABILITYCRITERIA "This toxicity test meets quality criteria provided by Azur Environmental Corporation 1). Tthwea9s5>% 1c.3 onfaitd3e0n-cmeinf;achtoowrefvoerrt,hethEisCwSaswparsoba<1b.3lyfcouretthoecomapnldet1e5-imnhiinbietxiopnoasturtheepheirgiohdess.t concentration tested (1000 mg/l) 2) Tahte3c0o-emfifni;cihenotweofvedre,tehrimsinwaatsiopnrwoabasbl>y0d.u9efotro ctohm5epleatned i1n5h-ibmiitnioenxaptotshuerheipgehreisotds. !t was < 0.9 concentration tested (1000 mg/L) 3) The correction factor was > 0.7 at min and > 0.6 at 15 and 30-min, This new version of Azur Environmental Corporation software does nol provide the correlation coefficient. This test was conducted by Trudy K. Stiefenhosfer as requested by Pam Woll of 3M Specialty Chemicals Division REFERENCES 1. Microbics Corporation. 1982. Microlox Manual. Volume 2. Detailed Protocols. Carlsbad, CA. 2. Microbics Corporation. 1993. Microbics Customer Training Manual. From February, 1993 workshop. C04286 MPiacgreot4ox May 12,1997 TABLE 1 SUMMARY OF TOXICITY OF P3025 TO MICROTOX PERCENT LIGHT LOSS" TmELST CONC, 5-Min. Exposure 15-Min. Exposure 125 a 22 250 15 25 500 26 47 1000 9 98 ECs 600 510 95% Confidence Interval (5-86200) (500 -530) St"alMisetoifcaatlnwmoetrheopldiucsaetdes peste mwotws 30-Min. Exposure 28 33 62 100 350 (240 490) wuwstes 04287