Document 6b1g2ea1971076GqNY8Ko7qx4
AR 226 - 0861
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: ArefmeirxrteudretocaonstPaiFnOiSng,pFeCr-f9i5uo,roorocatsanaescuolmfponoanteen,twhoficFhC-m6a0y3Fa.lso(1b-e Octanesulfonic acid) (CAS # 2795-39-3).
6R0e3mFa.rkCsu:rrenTtihnefo3rMmaptrioodnuicntdiiocnatleotsniutmisbaermwixatsure50o1f.0T.h5e%
test sample PFOS, 7%
is
FC-
dsuileftahtyel,e1ne.4g5ly%coXlanbutthyalnetghuemr,,812..49%5c%awlactieurm,a2ce%taatcer,yl0ic.0fo7a%meN-r(,34- % Alkylether
hCyhdiroorxoiadlel,yi0y.h0e3xa%mciinsi-u1m-c(h3l-oCrhiidoer,o0al.y5l%)-t3r,i5e,t7h-atnroliaamzian-e1,-a0z.on0i5a%adsaomdaintuamne
chloride, and 0.05% tolytriazole.
cThhaerafcotlelorwiiznegdscounmcmeantrryataipoplnisoesftiompaumriitxiteusr.e Dwaittha imnacyomnpoltetaeclcyurately reflect the toxicityof the fluorochemical componentofthe test sample.
METHOD
Method: OECD Guideline 209 GLP: No AYneaalrytCiocmapllmeotneidt:ori1n9g9:4 Dissolved oxygen concentrations, temperature, and SPtHa.tistical methods: Resultswere determined by calculationofthe % Tinehsitbiotirognainn irsesmpisroautriocne:ratAec.tivated sludge mixed liquor collected from the: Metro Wastewater Treatment Plant, St. Paul, MN. Test coDnidliuttiioonn:water: Millipore Mill-QTM water
RSyenftehreetniccesaenwdagtee:st spoelruOtiEoCnDprmeeptahraotdion: A stock solutionofthe 5re0f0ermegncien s1u0bsmtLan1cNe,N3a,O5H-,dicdhilluotreodphteon3ol0,mwLawsitphreMiplalriepdorbeyMdiilsis-oQlvTMing awantderd,iltuhteend tboro1uLghwittthotMhielip-oQiTMntwoafteirn.cipTihenet pprHeocfiptihtaetiroenfweirtehnc1eNsoHl2uStiOo4n was measured to be 7.5. Test substance was added directly to the
test vessels.
TNeusmtbveersoseflcso:ncNeonttrgaivteino.ns: one and a blank control, in duplicate, -
plus 1 reference substance
TToetmaplersautsupreen:de1d7s.o7l-i2ds02andC pH on day of testing: 4.1 g/L (TSS in
final: 1.6 g/L), initial pH 7.7 and final pH 7.1.
002476
Element Basis: Respiration inhibition as determinedby oxygen
consumption. Remarks: Values
corrected
to
20C
for
calculations.
RESULTS
---
Nominal concentrations:
Blank control, and 1000 mg/L, in duplicate. Reference substance at 10 mg/L.
Element values:
30-minute EC50 = >1000 mg/L 3-hour EC50 = >1000 mg/L.
Element values based on nominal concentrations.
Remarks: Substance
Testing was conducted on Remarks field. The values
a mixture reported
as described in the Test apply to that mixture and
not
the fluorochemical proportion alone.
CONCLUSIONS
Sp----
TdehteerFmCi-n8e0d3tFo3b-eho>u1r0E0C0 mgf/orL,atchteivhaitgehdessltucdognecernetspriartaitoinonteisntheidb.itiMonewaansvalue of 10.1% inhibitionatthis concentration. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY.
tReesltiianbgi.litHyo:weKviemri,scshamrpalnkeipnugri=ty2.wTahsisnosttupdryopmeerleytschtahreacctrietreirzieadfoarnqduatlhietystudy lacks analytical confirmationofthe amount of fluorochemical proportion in solution.
REFERENCES
e--r--------reesemeet
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number M1655, 1994.
OTHER
premises:
Last changed: 6/26/00
002477
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ENVIRCNMENTAL LABGRATORY ACTIVATED SLUDGEMXED LIQUOR
Type Test ce(n AGI Sadeg Regianicn Tani
ss
AbaniaeyaaSnES, Page Col Men Tet 230
ACTSI LUDV GEA MIXT ED LE IQUD OR
Date Obtained -\C Ym
Date Used _5-\C-9Y
Mixed Liquor Suspended Solids (MLSS)__\:\ g/L Adjusted o_o
MLSS in final test solution__ Ve gL
nial pH 21-7) (1st test soln. prepared)
Comments _
_--
2 Final pH L\_ (ast soln. prepared)
-
JEST SUBSTANCE
Sample Description _E(C Ler- senC d DT
Initial pH of highest conc. tested (LCCC nl) = LD
Adjuste1d pH
Comments
EEFERENCESUBSTANCE +
Sample Description _ 2,5 = Dic\\\oPrhe
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RAWDATA
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