Document 6ZkgzL0bGV0G0KQE3LQmQK6o
FE-RO
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity: aPPceFirdOf,lSu1o,Dr1oE,o2Ac,t2s.aa3nl,et3,s,uF4lC,f4-o,9n59a,,t5e,o,6r,D63,EM7A,7S,saa8lm,t8p;,l8me-ahNyeop.atla2ds.eocba(fe1l-urOoecrftoea-rn,reescduoltfmoopnadis.c: with 2,2"iminobislethanol] (1:1), CAS # 70225-14-8)
R(aepmparrokxism:atTeelsyt2s5am%ptleestissaubmsitxatnucree oafndth7e5te%stwastuebrs).tanAclevianlwuaetserreported
relate to this mixture. No calculations were made to adjust for the actual concentrationof the test substance inthetest sample.
METHOD
Method: EEnPvAi-r6o6n0m/e3n-t7a5l-0P0r9o,teAcptriiol,n A19g7e5n.cyS,tEacnodlaorgdicMaeltRheosdes.arch Series Type: Static acute
YGeLaPr:cYoempsleted: 1979
SSuppepcliieesr::LOespaogmeisCamtafcisrhoecrhiiers,usInc. in Osage Beach, Missouri.
AEnxaploystiucrael pmeorniiotdo:ri9n6g-:hopurHsand DO / ammonia content
Statistical methods: Probit analysis.
:
TLeesntgtfhishanadgew:elNgohttn:otAevd.erage length = 28.6 +2.17 mm.
Loading: 02g fish /L.Average weight = 0.60 + 0.15.
Pretreatment: None
Test coDnidliuttiioonnsw:ater: Laboratory well water
DilutioDniwsastoelrvecdheOmxiysgterny:: 9.3 mglL
hardness:
255 mglL as CaCOy
alkalinity:
368 mg/L as CaCO3
pH:
7.8
Conductivity:
50 pmhos/cm
Sdetioocnkizaenddwattesetrsaotlautcioonncepnrterpaatriaotnioofn:15P0rimmga/rmyLs.toTckheprteepsatred in concentrations were prepared by transferring appropriate aliquots
001566
of the stock standard directly to the test chambers. The test solutions were noted to foam when stirring in toxicant aliquots. Test concentrations were prepared based on total sample, not on percent concentration of the test substance in the test sample. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 40liter glass aquaria containing 30L of test sNoluutmiobn.eorf replicates: one
N Nuu mbm erb ooffe fciosrnhcepnetrrraetpiloincsa:te:sixtepnlus a negative control Water chemistry during the study:
Dissolved oxygen range (0-96 hours): . 60-8.4mglL (control exposure)
5.8-8.3 mg/L (18 mg/L exposure) pH range (0 - 96 hours)
82-83 (control exposure) 83-83 (18 mg/L exposure) Test temperature: Temperature held constant at 22C through use ofa water bath for test vessels.
RESULTS
Nominal Element
concentrations: value: 24-hour
BLkCscoon=tr4o6l,0
1(83,7307-,58705),
m1g6/0,L
320,
650
mg/L
48-hour LCs = 370 (296-470) mg/L
96-hour LCs=o 31 (22-43) mg/L.
96-hour NOEC = 18 mg/L (C.l. not calculated)
All element values based on nominal concentrations
Statistical Evaluation of Mortality: Probit analysis was used to calculate LCs; values and the corresponding
confidence limits.
Quality Check for Test Organism Health:
`The bluegill sunfish were challenged Antimycin A. The observed 96-hour
with LCso
aanrdef9e5re%ncceoncfoimdpeonucnedl,imits
(C.L.)
were within that the fish
the 95% were in
confidence limits good condition.
reported
in
the
literature,
indicating
001567
Cumulative percent mortality:
ToNto"nmCsoiatael.,
2000s
Lo To oTwT w]
Loo[www [ wm]
CONCLUSIONS
`The test sample mg/L with a 95%
96-hour LCso for bluegill sunfish was determined confidence interval of 22-43 mg/L. The 96-hour
to be no
31
observed effect concentration was 18 mg/L.
Submitter: 3M Company, St. Paul, Minnesota, 55133
Environmental
Laboratory,
P.O.
Box
33331,
p0 ata0 QuAU0 TY 0000000000
Ranealliyatbiiclailtyd:atKaiifmoirsdcehterramnikniantgi2o.n
This ofthe
stteusdty,suwbhsitlaenwceellcocnocnednutcrtaetdi,onlaicnktshe
gteasptssoiln utthieonmseaansdurdeetmeernmtinoaftiwoanteorf qtuhaelistaymcprliteerpiuarfitoyr.a Tnhuemrbeewreorfetahleso
concentrations at given time intervals.
REFERENCI
`This study Columbia,
was conducted by Missouri on behalf
Analytical of the 3M
CBoiomCphaenmyi.stry
Laboratories,
Inc.
of
OTHER Last changed: 5/2/00
--S--------------
001568
-
Submitted By: AC[72in%rrartlayntnti"ehcaeraxltHSiB0io0i7noooCuhnsesmist6r5y205Laboratories, Inc.
Prepaced By:
i
G[ECT HR aThboaE panonepre. 23To/s29
.
A Tras TOoleFar:
Ms<he
id
Aquatic Supervisor .
Approved By:
.
Tiesdoy lpdsvsasgee Qeficer D\e
EentotrysHaaser
-13i-
.
001569
sweuRy
~ -
The acute toxicity of 34 Sample No. 2 to bluegill sunfish (Lepomis
pmHaaerctarhmooecdthseirrfsuosr)ofTwoatxseimcapisetsryeastsTueerdset,ussdiwinisgtshotlhvAeeqdumaettoixhycogdesOnr,oguatpnililisnmaesndd (b1ay)m.mtohneiVaaCtoevmremrietqtumeaeelaisto-yn lred throughout the test and ere vithin acceptable limits.
-
As a quality check, the bluegill sunfish were challenged vith a
reference compound, Antimyein A. The observed 96 hour LCso and 95%
icnonftihdeenlcieteLriamtiutrse ((C2.),I.)inwdeirceatiwnigthitnhatthethe95%fischonwfeidreenciea lgoiomditsconrdeiptoirotne.d
8411 Tshuenfirsehsualrtes soufmmtahreizefodurbeldoawy. static fish toxicity study using blue-
Compound
-
3 Sample No. 2
Mntinycin A
96-9h5o%urC.1L.C3go (223-143mgm/gL/1) 0.000029 ng/L
(0.000021-0.000040 mg/1)
-v
tratiAolnsoo,f
1t8hesgr/els.ults
indicated
a
96
hour, -
no
observed
effect
concen-
001570
INTRODUCTION
- ---
tfoorry3HTohfiCsoAmnpsaatlnayytt,iiccfarlboinoBaiJsousCnaheyem1wi2assttroypeJLruafnboeorrm1ae6td,ora1it9e7s9t,,heIasancq.au,uatthiCocorliubzmibeoidaas,sinaNyiaslslaoebutortriea,r
vftaorsombtl3ouMedgCeiotlmelprmainsnyuestointshheJu2n(4eL,ip6,o4a8i1sa9n7d9ma9(c6ArpohpcoehunirdriuxLsC)gI.o). leTTvhheeelspstufurodpryos3e4wooSsfampptelherifsNoort.seesdt2
following the procedures outlined in ABC Protocol Nasber 7601
-
(Appendix I).
YETHODS AD MATERIALS
-
ods fTohreExparmoicneadtuiroens offorWastteartiacadbiWoaasstseawya,teras d(e3s)criabnedd iHnetShtoadnsdarodf HAceutthe
uTsoexdiciitny tTheisstsewxiptehriFmiesaht,. HaTchreoiabvleuregtielblratseusnfainsdh Ausmedpihn ith(eb1ti)e,sat nvveesrree
OFbitsahinweedrefriodneaOtsiafgieed cattofisspheecriieess,usIinncg. tihne OtsaaxgoenomBiecachK,eysMisdseovuerlio.pedTbhye
-
ELdidghyt (4p)h.otoAplelriotdestsndfisohbsweerrveedhelfdor inatculletausrte tfaonukrsteoenn ada1y6e hporuirordayt-o
testing. Fish culture techniques used were basically those described by
DBheornlatdusih,nngiespte.irnicoaldl.u,dead(l5)oi.nngAwpAiptedhnadiiplxryopI.rheyclDoaurcrdtiincogfstnfhdiissthhpeeorrbaispoeedru,vtaittchieodnisfsiesdahusrerientcgreeiatvthe-ed
-
T2eSsttainndgaradt covmhiecrhciatilnefisfheedfionogd (vRaasngdeins'cso)ntdianiuleyd.untTihle 4b8luheoguirlsl prsiuonrfistho
v. sTuiseeenndtgatthfiovorefth2gi8rs.o6upemxp.oefriFWmieesinhgthpthraidaoarda mlteoeanngtthwheebitegeahsstturoaefnsde0n.at6ts0evgeirannecdlumdaaedmdeeaoinnn Aasptparenendpdraeir-xd
.
contaiThneingst3a0ticlitfeirssh obfiolaasbsoaryatwoarsy wceolnlducwtaetedr.in C4h0arlaictteerrigsltaiscss aofquatrhiias
ailution water are shown in Table 1.
-
wfiitshhoTuvheteresfeoaocdvcels48ismehalotsuerdwsetrpoertikhoeerpttdoiilnutteasitoinvnagvt.aetrerbaatnhd attes2t2tCemp(e1r.a0t)u.re Tahned- hteesltd
(PinepBhaasleeds onprotnhcelarse)sulbtys 3oHf Cpormeplainmyi,narsiyx tecsotnicnegntwriattihonfsathoefadthmeinntoewsst
Coimophoupnedr, croanncgeinntgratiinona vleorgearistehlmeicctedserfioersdeffoirni1t8ivteo b6i5o0assmagy/.l, wThiethfitesnh
-
W5e0remiandudteeds taofttehre atdedaitticonhaboferstosbiycasnttraatliifqiueodtsr.andoTmhessussieguomfen4t0wiltihtienr
Fass aquaria for this study vas suthorized by 3H Company (Appendix 1).
conditTihoen.34 TShaemplseampNloe. u2posntarnedcaeridptwaswasrecoebisveerdveodn tJounbee 8&, y1e9l79l,owinligqouoidd
<0
-
TVaointddaeld'w;ascotsmhpteoorurenefddo.rea,tThrteohoemtetstetesmtpCeorcnaoctneucaretenrtart(ai2to2inosCn)s.werweeSraempprlepepraeprpueardrietbdyy bwtaarssaendnsofotenrprrtiohn-eg
Sppropriste aliquots of a stock standard directly to the test chambers.
-
2 001571
Twheerestnooctkedsttaondfaoradmvawshenpresptairrerdingin idneitoonxiizceadntataelri.quoTthse. teAsltl sosltuatnidoanrsd
~-
weights and dilution values are listed in Appendix I.
RESULTS
tervalTsablfeor23preSsaemaptlse tNhoe. p2readnidcttehde LrCesfoerevnacleuetsesatndag9a5i%nsctonAfnitdiemnycceiniAn,-
-
maetphiosdcicdiedsec.ribeTdhebsye LviatlcuhefsielwdereandobtWaiilnceodxonby(6e)m.ploMyoirntgaltihtey srattaetsi,stitceaslt
concentrations and water quality data are presented in Table 3.
saturTahteiodniswsaoslvceodnsoixdyegreend caodnecqeunattreatfioorn wtheistcihngs.tayTehdebpeHtwveaelnue4s0 arnedmai1n0e0d%
-
ctornastiisotnesntwerweitbheltohwe tchoenttrooxlicthlriomuitgho(7u1t). the study. The ammonia concen-
PractTihcee Rsetguudlyatwiasonscon(d8)uctaendd fthoellofiwnianlg trheepoirnttevnats orfevtiheewedGoobdy LAanbaolryattiocrayl
-
BdiaotCahewaaisstrpyrovLiadbeodrattoori3es'CoQmupaalniyt,y wAistshuraancceopyUnirte.tainAeLdL atoriAgnianlaylticraalw
BioChenistry Laboratories.
~N
3
~
001572
-
TBE 1
-
Chemical Characteristics of Vell Water at
ABC's Aquatic Bioassay Laboratory.
Parameter
Concentration
N
Dissolved Oxygen
9.3 ppm
ok
8.2
Hardness (CacOg)
255 ppm
Allinity (CaC05)
368 ppa
-
Conductivity
50 unhos/ca
Total Amonia (is)
<0.05 ppm
Koy
0.15 pp
:
Ortho-Phosphate
0.10 ppa
-
Alumina
<0.01 ppa
Arsenic
<0.001 pp
Cadmium
<0.001 ppm
Chromium
0.001 gpa
- te
Cobalt
<0.001 ppm
Copper
:
<0.01 pro
Iron
0.012 ppm
Lead
0.009 ppm
~
Mercury
Nickel
<0.0001 ppm
0.0157 pps
.
zine
<0.01 pou
ove
0 ng/1
~
Diazinon
<0 ag)
Disyston
20 ng/L
Methyl Pazsthion
<0 ng/1
Malathion
110 2g/1
~
Ethyl Parathion
<0 ng/L
"
001573
nz 2 The Acute Toxicity of 30 Saeple No. 2 484 Aatiagesa A to Bluegsll Suntish (Lepomis sacrochirus)
_comonns 1 Semple No. 2
Jatisycia av
L650 1a atlligrass/iiter (opm)
24 bows Grows (0.00000.3060-000.70000088)++
48 hous socsino (0.00000.3020-000.80000050)++
96 bours
|
aais (0.00000.3010-000.26500040)w+
"Boissay as conducted at 22C (1.0), mesn veight 43d leagth, 0.60 g aad 8.6 mm.
|
95% contidence taterval.
"smtacimycia standard obtained from Sigas Chemical Company, Type III, crystalline, Lot 1250-0152.
B@3ow
`
|
sheet
hte
A
as SE AT
ETD
rin
an Tpmn r -----T ram Concentration 26 48 96 C ws/l Et mg) C mg/l pi mg/l C mg/l pH mg/l
Cw a
e som em
@ wom om mse sa om PT
hrhei3n8 Pseeenmseesethon34=3i00t8oaeea0cn BpacCh eoe54.5 5)anwedeweitnh Ere e472 8 eo ste, sett meee settee fms Ste, teh oe SEA wih with, on Tre Wh 47 8 eo st 2ga#
LITERATURE CITED
~-
(1) C(oC.mmiE.tteSetepohann,MeCthhaoidrsmanf)o.r T1o9x7i5c.ityMetTheosdtss fwoirthAcuAtqeuaTtoixcicOirtgyanTiessmtss
wtietchtioFnisAhg,enMcya,croEicnovleorgtiecbarlatReesseaarncdh ASmeprhiiebsiaEnPsA.-66E0n/v3i-r7o5n-m0e0n9t,alAprPirlo,-
1975. 61 p.
-
(@) BSetrugdeire,s Bo.n LA.n,timRy.ciEn. ALeamsmona fainsdh J.toxVi.caHnotg.ea.U. 1S9.69.DepaLratbmoernattoroyf
Interior, lavestigations in Fish Control No. 26. 21 p.
(3) AtmheerEicxaanminPautbiloinc oHfeValattherAasnsdocWiaasttieowna.ter.19751.thSetda.ndaWradshMientghtoodns, fDCo.r 193 pu.
(4) Cdoemyp,anyS,amDaueblu.que1,9619A.. T2h8e6 Fpr.eshwater Fishes. 2ud ed. W. C. Brown
(5) BorfauRhens,eaJr.chL.Fiasnhd: R.RaAi.nbSocwhoTertotugte,r. Fat1h97e5a.d MAicanqouwiss,itCiohnansenld CCualttfuirseh
-
.
asnedarcBhluSeegriilelss.EPA-E6a6v0i/r3o-a7m5e-a0t1a1l, HParyo,te1c9t7i5o.n A4g5enpc.y, Ecological Re-
(6) oLiftcEhvfaielludt,inJg. TD.o,seJ-rE.ffaencdt F.ExpWeirlicnoexaotns.. 19J4o9u.r. APhSairmmp.lifEixep.d. MeThtehro.d 96:99113.
CT ---
(7) NUa.tSi.onaDlepAacrtamdeenmty ooff SCcoimemnecrecse., PB1-97213.5 1W9a9.ter5Q92uilp.ity Criteria, 1972.
(8) PFroaocdticaen.d DrFuegderAadlminiRsetgriasttieorn,. VoRle.gul4a3t,ionNso.for2.47G,ood599L8a6b-o6r0a0t2o5r,y
-
Deceaber 22, 1978.
7
001576
QTuoaxliictiyty Aosfsu3rancSeampSlteatNeom.en2t tfoorBlufeignialll rSeupnofritsh#(2L3e8p9o3miesntmiatclreodc,hir"uAsc)u,t"e
-
for Dale Bacon, 3M Company, St. Paul, Minnesota.
=
ductedIn ataccoourrdafnacceiliwtiitehs
AaBrCe
Ldaebsoirganteodriaensd
ifnutnecnttionthaitn
aclolnfosrtmuadnicees
conwith
0La0bdoraltaobroyratsotruydiepsr,actsinceinrsepgeucltaitoinonsofantdhethfeinaplrotroecpoolrst ffoorr i3n1diSvaimdpulael
-
Noou.r Q2uwaalistycoAnsdsuucrtaendceandUnifto.undAntoinbsepeicntiaonn aofcctehpetadbalielyfomromrbtyaliatmyemrbaetre ooff
wtehreeteisnt goorogdanhiesaalsthprainodr sthooutlhde niontitbiiaatsiontheofobtsheervsetdudmyoritnadliictayteidnthtehye
tsetusdtye.d haIdt avpatsbeaelnsosunpoptleidedt,hatthesiLnCcseo wtohueldpuhraivtey toof btehecaclocmuploautnedd bbaesiendg
-
oalnlnodmaitnaalancdonrceecnotrrdastioonnsJuonfeth2e0, to1t9a7l9,coimnpdoiucnadt.ed Athfaitnatlheinrseppeocrttionsuobf-
mbiyttAeBdC tLaobyoroautoirsieasn.accurate reflection of the study as it was conducted
-
in thSihsousltdatyeosuenthavoer atnhye qfuuenscttiioonnsofreloautrinQgualtiotytheAssiunrfaonrcmeatUinoint,prpolveiadseed
contact me at your convenience.
--
aA Ault.
Date79
Lity Assurance Officer
8
001577
APPENDIX 1
RAW DATA
001578
1
ANALYTICAL BIOCHEMISTRY LABS
~ BESTCOPY AVAILABLE
Aquatic Bioassay Lab Acute Toxicity Bioassay
Date: Time:
Sliz/ny & an
TFoofrfmeulaantto:r_C_o3mm-et2per DLaatbeNoa.:it 2 7
PTo ami: 1 r Av pLattaoe nraan dTi. ate
= oveeor Final Gr StWoeco kr SSts anndeaers dasg
WF orkihi g oq Sftsanou dmaeredno strat ted stock
NTeatrewiw.t. erie ceo E
Fe Dilution Vol.
----
CorniocnenvotirrBaatigo/m5n (Salance Calibration +) Comne114Cogrye]g = 5
S"ToeusrtcSepecies: Bien 4SedgSepleLieointt No. 1874 Temp:a Z3oC
Water ChemiSTrys
7
ALInTty
ardness 24
SrEeERTTon |
T 77hrw]
sere]
opreec [[ = | TolT [ToT lT T oT T [ST T1 T ]
REET
~
sel T TolTol[el Jo ||
N31 (74 | TolTol 1 Ts Pal
75 [wel | Tol Tel"Tal To
1
o lel[T Tol[o ZheelrT o| | |
- 320 lee | Teo| zl]jp Jo| | 4
em
TT TTT
bod
ll | 1jol Tijol[ol lo| [1
|
rrr
=
1
|
rrrr
rr
AA IY ES S HYHYHO A
=
rr rrr
Observ: IT Ter Ter Teg ye
Date
LTT Tega] bral loge] 12 ||
Remarks: ) bed 1 em Lr ivng
CoE = (bss of eqeitibrine
petpesebdu adding ledicated aliguafs ex taund ta Zo 0 aquaria -- ert wedenof voted Fo
Prdfared by: Eth or ~*ChoCrhreecckteeddWiftohr cpluarsistySowfeipgrhitmsary standard
hecked by:
ZL 001579
m Bre5.st suo e . r(e Fete|
ean TE TRALoa ng Us|56 [nisi[pes mg rs]S5 [Bagi sea a OE [eas pen airs
TT TT TTTTTTTT 1]
oermar [2x[51 [seis| 2e|galgelogncn[ne [63 [0lew
[60[53[sz
830g [oelxe [2s [73[3[en Isis [ea[pz
|
TTT FT
w [TT
TT
bo HE a ee er ee
ir
o
+ |g. Jodo
Cr TrrT 11
[T FT T T rrrT r
CT TT
LTT
rr rs
SNYH AI I~
fEsoneso more ameLanT - PBeResT OorT FnT GeT te 8TwT nt <1 i me tol
Boort s10 veet arotns eteoEtTa Ftne, (ehEoths thg,t$8o7d8 0m tSne Yoda 73 tk
11
.
-
ANALYTAqIuCaAtLicBIOBiCoHaEsMsIaSyTRYLabLoArBaOtRoArTyORIES
Acute Toxicity Bioassay
_~
"toxicant __3 m2
Probit AnalysiDsatWeortkestSehdeet Gla - b/io/2g
Test Species Blugay fu w Se Date reported [py7/29
Lab Nunber_._ 2 3gq3
Temperature____220C.
"Exposure period 24 [ig
Water quality well Hoo
Concentration
0
| No. dead ;
Observed %| Expected % lo 5 | contribution
|
total no. | mortality| mortality {OF
to Chi
~
: one lo
-- 1-3 --
1
o
ol o.evo
"
ET)or 0 ]
7
Ttil 0o.0
-
--i
1
i!
"ial onimals = 30
. Kk
= 9|
egy = _b GO
"ey, = AGO
.
Lie = _320
Sm10g JIC, + LoyiC, =
Total contribution to Chi_(_0_(D. chiz- conttoribchuition Xtotala%nimals= _[-O
oni? (p05) for (k-2)_L deg.ofFreedom = 5-H .
"5.0% so, 2z 4%%0 14% . Confidence limits (.05) for Less
"FLweN 2W 0 = [28 . 2 so =Aon
3l0-
Leo / F Lg = lower limit = 370
Lo3g X FL ,g = upper limit = EEO
Anatyvesis bybys log m - Aguetic.TrSiiesclyiit
Checked ey: ZH J]fb
vate: /fe/os
lf Gace
.
001581
11 ~
ANALYTICAL BIOCHEMISTRY LABORATORIES Aquatic Bioassay Laboratory Acute Toxicity Bioassay
i.
~loxicant
3m-2-
Test Species
Lab Nusber.
"e--x_po--sure period 48
.
Conceyntyragion
|
|
N
1 20
50
~
Ea
i
Probit Analysis Work Sheet
Date tested - 17 > Geld
;
iin
Date reported
/ 2/29
Temperature ___ 5 per
pese
e Water qy uality(exee2@Heeoo
No.
dead U7
toto
auserved 1|
no.| `moriatity|
Expected
moresticy
| | contrivution
Joos] to chi
of
[o
hye
o
0
i ol gee
Zz
zo
| o.
o
A
9
11.31 0.00
|
i1
o_ovr 1
Stel animals 30
K
----
. 1 = _490 "ie, 370
Total contribution to Chi_____0.0q
hi2- contribution Xtotal animls= 0.59
Chi (p05to)Chfior (k-2)_L Kdep. offreedom =_ZEL
tes = AZo $510, /1C,, +LS /16, =
Te BS "se tz e =) 32
Confidence linits (.05) for Lcsg
wMee__ J n RO TE 2 .
Lego / Ley = Tower limit = 290 Logo X LC, = upper limit = 470
.
ABhien
.
-STOmulfe1t0 ope-d)
.
~ Analysis by:_( arteSe Pega, Rioloy C7"
v
"
(i1ame)
Checked By: Jaf) Fork
(Title)
Dates gaps
Gag
(Date,
-
001582
11
~-
ANALYTICAL BIOCHEMISTRY LABORATORIES Aquatic Bioassay Laboratory Acute Toxicity Bioassay
a
~Toxicant,
Test Species,
m2
q
Probit Analysis Work Sheet
Date tested
A
Date reported.
Lab Number
"exposure period_Qp us
Temperature_
vater quality
Yn = fof
Ll (29.
2.2.%C
(0.000 Hoo
. Concentr0ation || No. dead ; total no.| Ombosretraveldit%y|| Emxopretcatleidt%y l1o9-2E!| conttroibcuhtiion
~
-- ~~ oO;
lo
JE py
|
|
aad
L
&
|
-
|
`190
!
5i :
:!
00 (9)!
lo! o
-- rrr
Tannl animals wo 4O Total contribution to Chi_____Q.2bn
.K
-4
a= Se
Chi2- conttroibChultion XtotalaK nimals= 2.960 oom" (pe.05) for (k-2) Zodeg. offreedom =
T= 31
tee= {8
$= 1g /LCy, + LE MIC, (=
at
> se K sz, iz3es apm0
Confidence limits (.05) for LCs
"Nfe_i_ gns2t0Nw = [39 .
Sa
ivy
:
.
QQ ke 65 = 3] gl .(22-4435mmys)
Lego 7 Leg=g lower limit = 22
.
LegXo F LCg=y upper limit = 43
.
~ Amalysis by:
.
Triana)
Checked: sy: LAD Jobe
ic Biola:
I
>
(Title)
(Bate)
vate: (frtsn
001583
C0 mie
sme mm "i
a.
,
--
i P :En Ee E ire LH e R JeE A enaL 88
:. d m TA ag ee 3 E
aE
ay = en
Ten J EE
EHH
ro/SeeLERHEE:EEEaa L on
2
E Biae Cf uonde sen
at
aa
Hite 1 Hf
wo
ETE
ANACATLBTUXTTLILSL(xY Lass
`Aquatic Bioassay Lab Acute Toxicity Bioassay
Date: 29
Toscant: LoAdland A
~Formulatory
1aateMMaadsee: lz,
a ~, _iy TTr iannek:s 00 p=
SolAmvt ent
A FiTnaaarleGwen g e 10 oE
WBolrikaitsntoogtnSotVaonrd,aorodns,ratetrat:edd Sstockhook _. 4
Net wt.
35g
FinalConcentration 6.0075~ Ti
- CDAdiojl.untcinoeentnvwtto.lrla7tdoioY6oo ng/g_m--mlgl* (Balance Cali2 brati5 on _v"a#4)
"eSTeorsutrciSepeecieDigs s: Fuell SediSsdliaev roLno:tNooe e. 457s 9 i sTe empj:jl aae za
ater Chem Sty:
2
:
:
DoesTope Tesd ota {Deaous:|Deadobs] DeerObs] vesay
Gk arg] | JO] lelTolTol[1]
pst
| Jo | TolTol Tol 1 1
seccos Alus|
| Jo| lel TolT/T11
--vodsose
olad
| Jo| Tol To
T7111
ale | {To | TelTa T=f 1 1]
elnl
| TolTol-TT =] HT1
cosose Ase] | | | 1g ol 21 1 1
else [To Ts TelTelT |
gocoe also
| Tel TolTolToT 1-1
ale]
| Tel TelTelTo
Hae ES CT T1T1
C11
rrr
CT
INNENASHN NNNNI
Toserv CT Tse = Teg Ter| ||
ate
LT TenT I lpAl Tosal Tope 11
Remarks: 0) event tnd pom ET 0/1
ee eeeeerie eee
PSroerrpecatredbey A Sob ET OTA e P chearea vy:L CAHN5 Amor --
Fichacrea ian Lars salons" Sone
001585
11
el.-
--
ANALYTAqIuCaAtLicBIOBiCoHaEsMsIaSyTRYLabLoArBaOtRoArTyORIES Acute Toxicity Bioassay
oe
Ioxicant.
Aetn
Test Species,
Lab Number,
Snposure period,
Concentratia on
0
= Commeoc"
0.000032
o0n00e0
~
-
}
Probit Analysis Work Sheet
c
A
Date tested - b! = o/t5/1a
0
gr
Date reported Glistag
Temperature 22.0C
24 he
Water quality_ f2pcon bro
i| No. dead ;
i
total
~~ Ono
|
9,0
|__to2/r100
i
/
i Observe%d| Expected %| | Contribuuttilion
no.| mortality| mortality |9-E! to chi
|
.
= 1= =
.
joi oc.oee
2
-
|
=
ol oeso
gz lial.
1
i
Tota:animals =
K
-
gy = Q-030dp
20
Total contribution to Chi_____0-046
3
Chi,2- conttroicbuhtion Xtota)Kanimsls=_4 0
. thi" (p=.05) for (k-2)_|deg: offroedon = _3.94
1c1go6 == 090.000005740
"
S LCG/LC, + Le i =
Tow2er
so
SR
2
emt
(29
Confidence linits (.05) for LCso
we__1O
.
Tle ms NW = (25.
eso/ LCgo = lower limit = guoowosi Logg X f LC, = upper limit =pasnF
|
CL
Bb 1650 BN
. = O2RTIOWY .
.
-
-
foooemo erally
~aalvsis by:Lad ollie A4 ug(iTitlee) Fiat ?
Checked: By: ZL, [) Jilin
Date: gfafra
H1: p
TTT
TT
01586
11o
ANALYTICAL BIOCHEMISTRY LABORATORIES
-
Aquatic Bioassay Laboratory
Acute Toxicity Bioassay
-
Probit Analysis Work Sheet
aoxiant, ArtimaginA
Date tested_- (4 --@l%/29
Test Species
it
Lab Number,
SSL bate reported bsg
Temperature___220C
Ewposure period' 4 hes
Water quality.
. Concentration
< orzo
:
L000
Loss
L000 :
T
=
1
T
|| No. dead / total no. pred Observed %| prt Expecte%d |Jos to Chi Contribution
iw Olio _-
I
or10
oO (li
2/
==]
1i
3 lol
--
000
owe
1| to, > |
4)r
T a1 io.
.
z
'
Tore animals =
30
.K
-
>
LCgy = 0.0008
XC, = Oooo
.
L616 = :00003)
~
S mC /IC + LC ie
Ji
i
[I
Total contribution to Chi______O-045
ChiL2~ econctrihbution Xtotalanimals= _O-4%
chi? (p-.05)for (k-2)_ deg. offreedon = J-84
:
.
Y=E 1090d0t ot2 co"Gwoz)a 1.30
.
.
Confidence liaits (.05) for Leso
No. Ie) Leg = 52-7 JN
= (126
.
4B writes
.
Les / 1650 = lower limit =0.000052
L040X F Leg=g upper Vimit =amveeSo .
nalysis by:
5
_
flame)
:
= ooredo. ngl Gromonazommesc
95 C. 1.
:
LL
te Bidloy:
7
(Titie)
(bate,
Checked By: F _ L pile D vate: dfufpa
EE
wt vv mv. am
001587
1
. . 4%.
ANALYATqIuCaAtLicBIOBiCoHaEsMsIaSyTRYLabLoArBaOtRoArTyORIES
Acute Toxicity Bioassay
-
Probit Analysis Work Sheet
Toxicant. Autiomacin A
Date tested _-_(Q[( -- &is/7g
Test SpeciesBloggit Sufi "8 vate repories__b/i57/
Lab Numb[er,brineeTyicant
Tenperature____ 22C
Exposure period
9 3 hes.
Water quality econ 20
.
Concentration
| No. dead
|
|
/ total ro.
Observe%d| Expected % | |conceibution ens | worcality |9F1 to Chi
< onTReC
Of
:
= -1 =
9-03000
t
2,
zo
i ol ozo
003
~ 0000
'
| 12710 | 102 fg)
| po
124i 0.020
.
i
1
i
_-- ri
Yor_ animals = RO Total contribution to chi_18_D _O-
:K
--_--3
Ly = Qosoeliz
cy = bosoozq
L036 = Q.000020
Sm 10 JIC, + LE JIC, =
3[pE ver-- g [44
2
Confidence limits (.0) for LCs
F wem( ST0 INT faagl.
Chi,2- conttroicbuhtion XtotalKanimals=_(-BO oni" (p05) for (k-2)_l deg. of freedom = SRG
i
. Qh ls =o
:
. AIR
oso/ F 1Cg=q Tower Vimit =p.omoz
.
.
LogoX F Lg=, upper limit =0:0%040 *
.
.
wnalysis by:
o
(lane)
Checked By: _ Har J) Ioubm
T-
Tie itlBeodog,
vate: {feafpa |
'S,(Gate)
oo
6v15%33
Te irteJ A --
mBeg Lr -Ga EIST cLE hH iaR
"LH TH HsbrcelmHH
J, EE --TL iE p rrC ETGi Si
7w hA m eELCE
" FE ae | Cd{ T S eea e a e L a Le = :
FESR
fr
Ta=
g
E
g 2 Ta pS LHi fh H(i H LnHS
: Eo PSoa
Moei ccan a
u
a
ea
AE
=
- SESTCOPY AVAILABLE
ANALYTICAL BIOCHEMISTRY LABS
AQUATIC BIOASSAY LAB
CC
he n
Test: 3M
Test Species:
source: (Og Group Measured:
"TEST FISH MEASUREMENTS
Sawples
Hl
| --9 , BLE Stehes
lsu.
Lot No.
Date Measured
iy
soe
Co
ee
:
1579
Glirlrg iy
< Fish No.
2 SLetnagntdhar(dmm)-
SS Wa eight
1
=
z
-- mS
lie 8
!
5
:
7
=
8
10 2
THeStaanndard
eHeiagnht
-
----
:
Length (mm)
@
9
-_ 0-68
27 :
0.42
| 23a0 = |oo-d %0 e
o
0. 58
30 -
-
.0.bz.
30
.
-o
. 0.6%
072 -
o
20 -
; oz
:
`0.40
_
__Z8L
:
(Sh=2.
.
-
0.60 (shz=0.15.
"Remarks:
_ Prepares Calg, pr Onecred ay: JL ) folie
, . 001590
: BEST COPY AVAILABLE, ABC Aquatic Research Laboratory
ish Requisition and Disposition Record
species: J, nf) Su fob
Date Rec: s/c/oq Lot #:~<L
source: Dodie (G45: hetes
No. Rec.: 000 Tank #:
A--
Use' Bioadisiyy
Comments -_-- _--
_--
Date
Le(nmmg)th
SDizie asndpositiReoconrd
We(igmg)ht
Used
Date
Le(nmgmt)h
We(igmg)ht
/57 i
OO
Us#
Irestment Record
te
Drug Used
Sones,
Time
Result
:
Daily Record
late | fDead| Comments | Date | #Dead| Comments | Date | #Dead|
Sesh
Sls dali el | |&) shppg,
Shot LenB
[ |5 Geld |]
Commer
Del 0 17Eg
eZ
I
wolo[TEA
1 o ["gasld |
1
(Jo | lolAgde[1