Document 6XJBpDprj942Q9jNaoKkDkD1

AR 226 _0821 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) IESTSUBSTANCE ~~~ 00000 Identity: A mixture containing perfluorooctanesulfonate; may also be referred to as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 101. The test sample is FC208CF. Current information indicates it is a mixtureof 0.5% PFOS, 10% diethylene glycol monobutyl ether, 78.08% water, 1.375% acrylic foamer, 1.5% sodium octyl sulfate, 1% sulfonated alkyl ether salts, 6.0% urea, 0.5% triethanolamine, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicity of the fluorochemical component of the test sample. ME0 THOD:000000 Method: OECD Guideline 202 Type: Acute static GLP: No Year completed: 1991 Species: Daphnia magna Supplier: In-house cultures maintained at EnviroSystems Division, Resource Analysts, Inc., Hampton, NH. oxygen. Analytical monitoring: Temperature, pH, conductivity, and dissolved Exposure period: 48-hours Test organism age: <24 hours old Statistical methods: No calculations performed, based on study results. Test conditions: Dilution water: Filtered natural well water - Dilution water chemistry: Conductivity: 840 pmhos/cm PH: 76 Lighting: Cool-white fluorescent lights at 20 pEin/sec/m?. A daily photoperiod of 16 hours light and 8 hours dark was maintained throughout the testing period. Stock and test solution preparation: A primary 1000 mg/L stock solution `was prepared and added directly to create test solutions. Exposure vessels: 250 mL beakers containing 200 mL exposure solution at a water depth of approximately 6 cm. Number of replicates: 4 Number of daphnids per replicate: 5 001876 NWautmebrecrhoefmicsotnrcyendturraitnigontsh:e five plus study: a blank control PH range (048 hours): 7.6 - 8.3 (control exposure) 7.7-8.3 (1000 mg/L exposure) Temperature range (0-48 hours): 1199..01--2200..23 CC ((c1o0n0t0romlgelxLpoesxuproes)ure) Conductivity range (0-48 hours): 840 850 -- -- 880 880 umhos/cm umhos/cm (control exposure) (1000 mg/L exposure) Dissol7ve.d68ox.y3gmegnlrLa(ncgoentr(o0l-4e8xphoosuurrse)): 7.78.3 mg/L (1000 mg/L exposure) R0 Eswrs 00000 Nominal concentrations: Blank control, 150, 250, 400, 600, and 1000 mg/L. Element values: 48-hour ECs = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test tShuebsftluaonrcoechReemmicaarlksprfoipeolrd.tioTnhealvoanle.ues reported apply to that mixture and not conclusions 00000 The test substance 48-hour ECs; was determined to be >1000 mg/L. No mortality or sublethal effects were observed. `PSauubl,miMtitenrn:esot3aM, C5o5m1p3a3ny, Environmental Laboratory, P.O. Box 33331, St. . p0 ataQu0 ALyY 0000000 tReesltiianbgi.litHyo:weKvieirmi,stchhersanakmipnlge=pu2r.iTtyhiwsasstundoyt mpreoeptesrltyhechcarriatecrtiearfiozredquaalnidtythe study lacks analytical confirmation of the amount of fluorochemicalproportion in the solution. R0 EFEREN0 CES 00000 This test was conducted by EnviroSystems Division, ResourceAnalysts, Incorporated, of Hampton, NH at the request of the 3M Company, St. Paul, MN, Lab Request number J1884-4, 1991 001877 FE Last changed: 6/28/00. 601878 R } : 5 SRRIDE CERI IH, 4 2 RS RRA 5, i3E BE REAR Te on Re i EF Sa BEST COPYAVAILABLE : Hs ; staty pinta statteic utBanTeotietSiyopofarseo-rneascr Si Pm Gras 2 Se SFiieSR RAE , IRE Tinothy J. Ward Robert L. 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