Document 6X6RXLked1kVGdp0dMrgYLv4

y^e slsl& _ o z r i TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603EF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number is not noted. The test sample is FC-603EF. Current information indicates it is a mixture of 1.30% PFOS, 10% diethylene glycol butyl ether, 54.15% water, 22.5% ethylene glycol, 2.6% propanesultone foamer, 5% Alkylether sulfate, 0.1% N-(3Chloroallyl)hexaminium chloride, 0.9% Xanthan gum, 0.9% starch, 1.0% triethanolamine, and 0.05% tolyl triazole. The following summary applies a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity o f the fluorochemical component of the test sample. M ET HOD;_________________________________________________________ Method: NS-EN ISO 10253,1998: Water quality - Marine algal growth inhibition test Type: Acute Static GLP: No Year completed: 2000 Species: Skeletonema costatum Source: Strain NIVA BAC1, stock culture maintained at RF - Rogaland Research, Stavanger, Norway. Element basis: Estimate biomass by measuring fluorescence Exposure period: 72 hours Statistical Methods: Growth rates by Log linear regression on biomass (fluorescence) vs. time, as described in NS-EN ISO 10253. Calculation of NOEC done by single factor ANOVA followed by Bonferronis procedure. Analytical monitoring: pH Test Conditions: Algal Nutrient Medium: Filtered natural seawater (34 ppt salinity) from an unpolluted site in Byfjord, Norway, enriched with nutrients according to ISO 10253 and recommendations from the Paris Commission. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was 8.2. Stock and test solution preparation: A 1020 mg/L primary stock solution was prepared by diluting 1.020 g of test substance in 1 L of growth medium. 002339 Aliquots were then added directly to growth medium in test vessels to create test solutions. Exposure vessels: 100 ml flat-bottomed flasks containing 50 ml test solution. Agitation: Shaken continuously on an orbital shaker. Number of replicates: 3 Initial cell loading: 2.0 x 103 cells/mL Number of concentrations: six plus a blank control Lighting: Continuous fluorescent lighting at >50 pE/m2 sek. Water Chemistry: pH range (0-72 hours): 8.2 - 8.2 (control exposure) 8.2 - 8.3 (1000 mg/L exposure) Test temperature range: 20 2 C RESULTS______________________________________________ __ Nominal concentrations: Blank control, 3.2, 10, 32, 100, 320, and 1000 mg/L. Reference substance (3,5-dichlorophenol) at 1.5 mg/L. Algal Growth Response ErCso values (based on growth rate) 72-hour ErCso = ~170 mg/L 72-hour NOEC = 100 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not conducted. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test sample exhibits a 72-hour ErC50 growth rate value of ~170 mg/L. The 72-hour No Observed Effect Concentration (NOEC) is 100 mg/L for growth rate. Validity criteria fulfilled. Control cell density increased by a factor of more than 16 in the test period and reference substance was within normal range. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY____________________________________________________ 002340 Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fiuorochemical proportion in the solution. REFERENCES________________________________________________ Test was conducted by RF Rogaland Research, of Stavanger, Norway at the request of 3M Norge, Lab reference number 99312-4, 2000. OTHER______________________________________________________ Last changed: 6/26/00 002341 L99ab3.1r2e-f4.no.: Lage 1 of 31 101 NORSK AKKREDUERING .Nil ROGALAND RESEARCH "FC-603 EF Light Water TMATC TM3%" Toxicity tests Report RF-2000/004 Lab. ref. no.: 99312-4 No. of pages: 31 ISBN: Author: Elin Horve Clients): 3M Norge Distribution restriction: Version No. / date: Vers. 1/1 4 .0 1 2000 Research Program: ... .. Open from (date): . Summary: The following test results were obtained with "FC-603 EF Ught W ater TM ATCTM 3%" : __ Ik Toxicity: Skeletonema costatum 72 h ECM (mg/l) : -1 7 0 Acartia tonsa 48 h LCM (mg/i) : 280 Corophium volutator 10 d LCM (mg/kg dry weight) : >3060 ^ The results relate to the product as supplied by Novatech. Key-words: i. Toxicity, Skeletonema costatum, Acartia tonsa, Corophium volutator. -.'-X ''i j.tv 0V $ ----- No part of this document may be reproduced in any form without permission in writing by RF - Rogaland Research . Istudydirector Inger Lisa Andersen for RF - Rogaland Research Kre Netland : -, 002342 99312-4 CONTENTS: 1. Preface................................................................................................................................... .......................3 2. Test for toxicity with Skeleto nem a c o sta tu m ...........................;............................................. 4 3. Test fo r toxicity w ith A cartia to nsa.......................................................................................................10 4 . Te st fo r t o x ic ity w ith 15C o r o p h iu m v o l u t a t o r ................. ......................................................................................... 5. Da ta sh eets...............................................................................................................................................20 002343 L99a3b1.r2e-f4.no.: Page 3 of 31 PREFACE: Ecotoxicological tests were performed on "FC-603 EFLight Water mATCTM 3%" in agreement with Novatech. The sample is a concentrate that normally will be used in a 3% AFFF/3% AR solution. Tests were conducted in accordance with the "Harmonised Offshore Chemical Notification Format"(OSPARCOM1995 /S F T 1998). The project was carried out at RF - Rogaland Researchfor 3M Norge. All the tests were performed by RF - Milj0lab. Study director was Senior Engineer Inger Lisa Andersen. The test results are presented in separate sections. The results relate to the product as supplied by Novatech. This test report is issued by a laboratory accredited by Norwegian Accreditation (NA). The accreditation states that the laboratory meets the NA requirements concerning competence, equipment and working proceduresfor all the test methods contained in the accreditation. It also states that the laboratory has a satisfactory quality assurance system, which includes internal quality control schemes, participation in interlaboratory comparisons, and the use o f certified reference materials and secondary reference materials. The tests performed are covered by the accreditation. This test report may not be reproduced in anyform other than infull, without the permission in writing by RF - Milj0lab. 002344 L9a9b3.1r2e-f4.no.: Page 4 o f31 Test for T oxicity Marine alga: Skeletonema costatimi 002345 L9a9O31.r2e-J4.no.: rage J o/jJ TEST REPORT Grow alga tShkienlheitbointieomn taecstoswtaitthutmh e m a r in e (S l NORSK AKKREDFTERING Nr.mt ROGALAND RESEARCH TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The 72 hours EC50was calculated to ~170 mg/1. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: ~ METHOD: NwSit-hESNkeIlSeOton1e0m2a53c,o1s9ta9t8u:mWaantderPqhuaaeloitdya-cMtylaurminetraiclgoamlugtruomw.th inhibition test TEST ORGANISM: SorkgealentiosnmemisakceopsttaattuRmF, -stRraoignaNlaInVdARBesAeaCrc1h.. A stock culture of the test ,,u:> PREPARATIONS: Natural seawater (salinity: 34 %o) from an unpolluted site in Byword was used as basis for the growth medium. The water was filtered through a GF/C filter, and briefly heated to about 75C prior to use. The seawater was enriched with nCuotmriemntisssaiocnco. rding to ISO 10253, and recomvmendations from the Paris Tmmegesd/t1isuwomalus.tpiOorntehpseawrreecdroenbcpyernsetptirraarrtienidognfrs1o.w0m2e0raegsprtoraemcpksasrooeflduthftrieoonpmr. otAhduissctstoticonkck1solsiloturluteitooinofngo.rfo1w0t2h0 INCUBATION: ToTlarehtbeseitdttacetuloslstthauwbarkaoesuesrtro,u2f3n05r0u0e0npmdclieelcrlawlscte/eomrsneftloiinfnrrucoeuomabucashatnlecigdoehnxintcpe(o1n>n0t5er0n0amttiipoalEnlfll/.yamtTg-2rbe-ossoewttktcio)numfglrtueoindrmoefsclfaulwuslukeormsreeosicnncueoalntcnutu-re--^ tubes of universal natural white type, at a temperature of 20 2C. Tbthyheeftlteuesostrtfewlascaseksnsrcueena.cfphoHrdaambyoefuaostru7gr2reomhwoetunhrtssm,waeanersduesrpueebmrsfeaonmrtmsp.elAedslagosafli5ngdmrioclwawttehedrweoantaskdmeanteaafsrsuhormeeedt. 002346 L99a3b1.r2e-f4.no.: Page 6 of 31 DATA ANALYSIS: CALCULATIONS: Gverroswusthtirmatee,saws edreesccrailbceudlaitnedNbSy-EloNgIlSinOea1r0r2e5g3r.ession on biomass (fluorescence) ErCso: The inhibitory effect on the growth of Skeletonema costatum is expressed as the ce3of2fn0ecmcetngct/orl.natcieonntroaftiaonprwodauscctaglcivuilnagted50b%y irnetdeurpctoiloantioonf gbreotwwtehenraltOeO(EmrCg/s1oa).ndThis NOEC: Calculation of NOEC (No Observed Effect Concentration) is done by single factor ANOVA followed by Bonferronis procedure for comparing each concentration mean with the control mean. TEST RESULTS: COMMENTS: VALIDATION: Table 1. EC and NOEC values obtained with Skeletonema costatum. Test substance W"FCat-e6r0TM3 EAFTLCigTMht3%" EfCio - ErCso E A o -170 100<EC9o<320 NOEC: 100 VGVaaralluupeehssPainarddeiIccnaaSltcetauctloa3nt.e0cd.enbtyraintitoenrps ogliavtiinogn.10N%O, E50C%araencda9lc0u%laitnehdibbyitiuosninogftghreowcothmrpauttee.r pUrongitrsaimn mmge/L The results are based on growth rates for the time span 0 - 72 h, the period during which the growth is considered to be exponential in the control cultures (see growth curves). Tcehlel dveanlisdiittyyicnrcirteeraisaedgibvyenaifnactthoertoesftmporroetotchoalna1re6fiunlfthilleetde,stinptehraiotdthe control (corresponding to a growth rate of more than 0,9 d'1), the control pH did not v3a,5r-ydbicyhmloororephtheannol1w,0ausninitsthdeurrainnggtehneotremstaallnydfothuend7.2 hours test results with Lab.ref.no.: Sample received.: Test period: Report date: 99312-4 20.09.99 07-10.12.99 10.01.2000 EtXli-nLHoVr\vOeAo. geMolversmyr ^ Engineer Senior Research Scientist^ Analytical technician Quality Assurance- ~ Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaultabthoerapteorrmy iascsciorendiintedwbriytinNgobrwyeRgFia-nMAiclcjpreladbit.ation (NA). The report may not be reproduced in any 002347 L99a3b1.r2e-f4.no.: l' g e 7 oj 31 PRIMARY DATA: Growth inhibition test with Skeletonema costatimi Test substance: FC-603 EF Light Water TM ATC TM 3% Lab. ref.: 99312-4 Start date: 07.12.99 Cone, unit: mg/1 Measurement: The biomass was measured by fluorescence: values in fluorescence units Time: 07.12.99 08.12.99 09.12.99 10.12.99 Concentration 14:50 09:30 10:25 11:55 Growth pH pH rate Corre- at start at end day-1 lation of test of test 0 0.12 0.26 0.93 4 1.22 0.998 0 0.1 0.34 1.7 9.7 1.59 1.000 0 0.1 0.33 1.1 6 1.42 0.998 8.2 8.2 0 0.09 0.3 1.1 6.4 1.48 0.998 0 0.09 0.26 0.88 6 1.46 0.995 0 0.09 0.26 0.9 7 1.51 0.994 3.2 0.09 0.43 1.75 11 "1.67. 0.998 3.2 0.09 0.28 1.2 6.3 1.48 1.000 8.3 8.2 3.2 0.08 0.23 1 4.7 1.42 1.000 10 0.08 0.4 3.4 14.5 1.81 0.995 10 0.09 0.4 2 11.5 1.69 0.999 8.3 8.3 10 0.1 0.4 2.2 12 1.66 1.000 32 0.1 0.28 2.05 9.3 1.57 0.998 32 0.1 0.3 1.9 9.2 1.57 0.999 8.3 8.2 32 0.11 0.3 1.65 8.8 1.52 0.999 100 0.09 0.31 0.77 5.4 1.42 0.991 100 0.11 0.35 0.75 5.1 1.33 0.986 8.3 8.2 100 0.1 0.36 0.78 5.4 1.39 0.987 320 0.07 0.04 0.03 0.04 0.00 320 0.09 . 0.03 0.01 0.04 0.00 320 0.06 0.04 0.02 0.06 0.00 8.3 8.2 1000 1000 1000 0.09 0.07 0.08 0.05 0.09 0.03 0.03 0.05 0.1 0.03 0.03 0.02 0.00 0.00 0.00 8.3 8.2 Reference substance (3,5-dichlorophenol): 1.5 mg/1 0.09 0.15 0.15 1.5 mg/l 0.08 0.1 0.12 1.5 mcj/l 0.08 0.14 0.18 Mean values: 0 3.2 10 32 100 320 1000 0.10 0.09 0.09 0.10 0.10 0.07 0.09 Reference subst. 0.08 0.3 0.3 0.4 0.3 0.3 0.0 0.0 0.13 1 1 3 2 1 0 0 0.2 0.2 0.22 0.2 7 7 13 9 5 0 0 0.2 0.28 0.908 0.35 0.968 0.32 0.921 1.45 1.52 1.72 1.56 1.38 0.00 0.00 0.32 0.998 0.999 0.999 0.999 0.988 0.969 The result of the reference substance (22% inhibition) is within the range normally found 002348 L9a9b31.r2e-f4.no.: Page 6'oj 31 GROWTH CURVES: Growth inhibition test with Skeletonema costatum Test substance: FC-603 EF Light Water TM ATC TM 3% Lab. ref.: 99312-4 In biomass (fluorescence) In biomass (fluorescence) In biomass (fluorescence) In biomass (fluorescence) 7 1000 mg/l 53 1 -1 24 48 72 .3 T ^ ~ IBM um Jju tH h ou isf, 002349 L a b .r e f.n o .: 99312-4 Page 9 of31 Inhibition of the growth of Skeletonema costatum with: mg/1 ( FC-603 EF Light Water TM ATC TM 3%) Experimental data: Concentration in Growth rate FC-603 EF Light Water TM ATC TM 3% Control 1 Control 2 . Control 3 Control 4 Control 5 Control 6 Control mean : 3.20 3.20 3.20 10.00 10.00 10.00 32.00 32.00 32.00 100.00 100.00 100.00 320.00 320.00 320.00 1000.00 1000.00 1000.00 1.22 1.59 1.42 1.48 1.46 1.51 1.45 1.67 1.48 1.42 1.81 1.69 1.66 1.57 1.57 1.52 1.42 1.33 1.39 0.00 0.00 0.00 0.00 0.00 0.00 -- : Inhibitic in percer . - - - 0 0.0 0.0 1.8 0.0 0.0 0.0 0.0 0.0 0.0 1.8 8.1 3.9 100.0 100.0 100.0 100.0 100.0 100.0 : 002350 L99a3b1.r2e-f4.no.: Page 10 o f31 niS 'O0 ro!j. -i T e s t f o r T o x ic it y -ob Marine herbivore: Acartia tonsa 002351 L9 9a 3b ).r2e-f4.n o .: Page 11 o f31 TEST REPORT ACUTE TOXICITY TEST WITH Acartiam a r ine co pepo d TtoHnEsa TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: I The 48 hours LC50was calculated to 280 mg/1. r The test substance was supplied by Novatech, and the test results relate to this product. f& TEST DETAILS: [ METHOD: ItSoOm/DarIiSne1c4o6p6e9p-od1s99(C7:oWpeaptoedraq,uCarliutysta-cDeaet)e. rmination of acute lethal toxicity ; TEST ORGANISM: Acartia tonsa University of (Dana), supplied Copenhagen. by the Marine Biological Laboratory, CULTIVATION: The test organisms were supplied as eggs, and were cultivated in accordance wanitdhatdhuelt"sPwareirseCuosmedministshioente-sRt.ing Test Protocol" (VKI). Large copepodites PREPARATIONS: Natural pasriborastios usfeosarew.thaetedril(usatiloinnitwy:at3e4r.%To)hferowmataernwunaspofillltuetreeddstihteroiungBhyawGorFd/Cwafisltuersed Tmweagst/t1ersw.oalOusttpihorenerpscaworneedcreebnpytrrseatptiriaorrniensdgwfr1eo.r0me00pa2rsegtporacarkmedssoforluof tmtihoenthp. irsAosdtsuotccoktciknsosl1oullutiittorineon.o of 1000 f dilution 1 Incubation: 1 T2v5ihaemlstlewsoetfrwteeakssterpsuotnluunitnidoegnrlaaasns1d6c5ohn/a8tnahiimnliegarhlsst/,(d24a8rrkemppllihrcooatltlbeepsdefroriirmodeavacitahalsc)toenemacpcehnertcraoatuntirtoaenion. fiTnghe 2d0ata s1heCet.. pH and oxygen measurements were performed as indicated on the 002352 L99a3b1.r2e-f4.no.: Page 12 of 31 DATA ANALYSIS: CALCULATIONS: The effect on the survival of Acartia tonsa is expressed as the concentration of paeprrtoedsut cctolnecthenatlrtaoti5o0n%weorfethuesetdesftoarnciamlcaulsla(tLioCn5.0)T. hTeh2e4chomoubrisnaenddm4o8rhtaoluitrys LstCa5n0davradlupersocaenddutrheeP9R5%OBcIoTnAfidneanlycseisli,mNiattwioenrael cSawlceudlaisthedEbnyviurosinnmg etnhteal Protection Board (Version 2.3,1990). This programme also calculate test concentrations lethal to 10% and 90% of the test animals. NOEC: CfcaoacnlctcouerlnaAttriNoantOiooVnfANmOfeoaElnlCoww(ieNthdotbhOyebBcsooenrnvtfreeordlrEomnfefiesacnpt.rCocoendcuernetrfaotriocno)mispadroinneg beaycshingle- TEST RESULTS: Table 1. LC and NOEC values obtained with "FC-603 EF Light Water TMATCTM 3%". Acartia tonsa 24 hours 48 hours LC10 280 (130 - 380) 89 (54-120) LC 610 (490-790) 280 (220- 360) LC90 >1000 880 (620-1500) ' NOEC: 320 180 ctVhaaellcucueolsmaitnpedduitbecyraptPerRo1gO0r%Ba,mIT5m0a%enaGalyrnsadips,9h0aP%naddl9eInt5hS%atalcittoy3n.a0fif.dteern2c4e ianntedr4v8alhsoaurres.indUicnaittsedin. mNgO/LECVaarlueecsaalcreulated by VALIDATION: The validity criteria given in the test protocol are fulfilled, in that the dissolved oxygen concentration at the end o f the test was greater than or equal to 4 mg/1, the percentage mortality in the controls was less than 10%, and the 48 hours LC50of 3,5-dichlorophenol (reference) was in the range normally found. Lab. ref. no.: STRaeemsptopprlteerdriaoetdce:e:ived.: 99312-4 1007-2.10021..0.12910..990990 AnalyEEtliicnnagHlintoeercevhrenician SenQiourgaRelieMtsyeoAalrvsceshursrSmacnyicerent1ist Enclosed: Datasheets Tfohrims toetshterrepthoarnt iisnisfusulle,dwbityhoaulatbthoeraptoerrmy aiscsciorendiintewd briytinNgobrwyeRgFia-nMAiclcjpreladbit.ation (NA). The report may not be reproduced in any 002353 L99a3b1re2f-.4no.: Page 13 o f31 PRIMARY DATA: Acute toxicity test with Acartia tonsa Test substance: Lab. ref.: Start date: Measurement: "FC-603 EF Light W ater TM ATC TM 3%" 99312-4 10.11.99 Number of dead Concentration (mg/l) Control 32 56 100 180 320 560 1000 Total number Number of specimen pr. vessel Specimen A C E pr. cone. B D F 5 56 30 4 5 5 5 55 20 5 5 55 20 5 5 55 20 5 5 55 20 5 '5 6 5 20 4 6 54 21 6 5 55 20 5 NUMBER OF DEAD 24 hours 48 hours ACE BDF Total ACE BDF Total 0 00 001 0 00 0 0 00 1 0 00 000 0 00 0 0 00 0 001 00 1 0 00 1 1 11 11 4 0 00 0 0 11 1 2 12 01 0 24 32 6 8 2 13 3 4 54 3 5 33 95 5 55 16 5 16 20 Reference substance (3,5-dichlorophenol): 1.0 mg/l 5 55 1 10 331 30 645 0 01 3 0 11 The result with the reference substance is within the range normally found. 9 002354 L9a9b3.1r2e-f4.no.: Page 14 of31 Test substance: "FC-603 EF Light W ater TM ATC TM 3%" Lab. ref.: Start date: 99312-4 10.11.99 Measurements o f oxygen and pH. Start Concentration (mg/l) Oxygen (mg/l) pH 24 hours 4 hours Oxygen pH Oxygen (mg/l) (mg/l) Control 7.3 8.2 7.4 32 7.6 8.1 7.1 56 7.7 8.1 7.1 100 7.8 8.2 7.1 180 7.7 8.2 6.8 320 7.7 8.2 6.8 560 7.6 8.2 6.5 1000 7.6 8.2 6.4 Reference substance (3,5-dichlorophenol): 1.0 mg/l 7.3 8.2 7 .4 . pH 8.2 8.2 8.2 8.2 8.2 8.2 8.1 8.1 8.2 | 002355 L99a3b1.r2e-f4.no.: Page 15 o f31 T est for toxicity Marine Amphipode: Corophium volutator 002356 L9a9b3.1r2e-j.4no.: Page 16 o f 31 TEST REPORT MACAURTINEETAOMXIPCHITIPYOSDEDCIMorEoNpThTiElSlTtttWVITOHlTuHtdEtO V TEST SUBSTANCE: "FC-603 EF Light Water TM ATC TM 3%" TEST RESULT: The lOd LC50was calculated to >3060 mg kg'1dry weight sediment. The test substance was supplied by Novatech, and the test results relate to this product. TEST DETAILS: METHOD: ASTM E1367-90, Standard Guide For Conducting 10 day Static Sediment Toxicity Tests with Marine and Estuarine Sediments. Test organism: Corophium volutator, from an unpolluted site near Trondheim. CULTIVATION: The test organisms were supplied as adults (4-8 mm in size) and were held in aerated seawater at 15C. A small amount o f natural seaweed was used for feeding. PREPARATION: NStaatvuarnagl eserawwaasteurse(sdalfionritdyi:lu3t4io%now) afrtoerm. an unpolluted site in Byfjorden outside Sediment collected at an unpolluted site was used for preparing test sediments. The sediment is a muddy sand and was analysed for organic content and proportion silt for each test. Each test concentration was prepared by adding the product in 600 grams of damp sediment in a plastic bucket. 150 ml o f seawater was added and the bucket was shaken for 3 hours. The sediment was divided into three beakers, 4ntcha0igel0chdumta.lamlt1oep0dfsasaeendsaiimwmmaagetlnsekrtwgww'e1raasessedtaahindmeadneleynrdsatetnddodrefoyoamwrchlwey,iagastheneltdre. cctthoenedtbefenoartk, eaearncsdhwabelelracekoaenercr.eanAtetrdsaaotmivoenprsleaoref INCUBATION: The test was run in glass containers (600 ml) each containing 200 g of test sceodnicmenetnrta,taiopnp.. 4T0h0embelaokfesresawwearteerkaenpdt u1n0daenricmonatlsin, 3uoruesplaiceraatetisofnoarteaach tinemdipceartaedtuorenothfe15datalshCe.etp. H and oxygen measurements were performed as 002357 L9 9a 3b ./r2e-f4.n o .: Page 17 of31 DATA ANALYSIS: CALCULATIONS: Ttmvrahaolteurioteeanflafioentycdftpatoehpnrertot9ehd5se%ut cscutocrlnoevcntiehvfniaadtllretaonotfci5oCe0nol%irwmoeopirtfheatiurhuemesectdaevlsocftoluuarltnacatiamteoldcraublislsya(teLuixosCpninr5.0eg)sT.tshheTedehsa1et0ascntdohdamaeyrbcdLoinCnec5de0n PROBIT Analysis, National Swedish Environmental Protection Board (Version 920.3%, 1o9f9t0h)e. tTeshtisanpirmogarlsa.mme also calculate test concentrations lethal to 10% and NOEC: CcfaoacnltccouernlaAtrtNiaotOnioVonfAmNfeOoalnEloCwwi(etNhdotbhOyeBbcsooennrtfvreeordlromEnfeifsaenpc.troCcoendcuernetfroarticoonm) ipsadroinngeebaychsingle- TEST RESULTS: Table l. LC and NOEC values obtained with "FC-603 EF Light Water TM ATCTM 3%" Corophium volutator 10 days LCio 99 ( -760) LC50 >3060 LC*, >3060 NOEC: 3060 cVVaaalllcuuueelssaaitnerddeibccyaatltechue1l0ac%otem,d5pb0uy%tePrRapnOrdoBg91r0Ta%manmlaeetlhyCsailsri,atyapahnfPdtea9rd51%I0nScdotaanytfs3i.d.0eU.ncneitsinintermvgalksga'1reseinddimiceantet dd.ryNwOeEigChta. re VALIDATION: Toxhyegveanlicdoitnycecnrittreartiiaongivatenthienetnhdeotefstthpertoetsotcwolaasrgerfeualtfeirlltehda,ninortheaqtutahletdoi6ss0o%lve, d tmhoerptaerlictyenintagaeremfeorretnalcietysuinbtshtaenccoenotrfo2ls0.w9emreglFeslusotrhaannth1e5n%e.kgT*h1edrpyerwceenigthagt e sediment was 16.7%. The sediment used is found to be within accepted limits fosoebrdsieomrrvgeeandnt)iacbscuiogtnntthiefenicmtabnourtttmatlhoiterytsaiinllitttyfhrieanchtoiignohneecissotnrcecocenoncmternmattrieoanntdioe(n1d0wh6i0agmshengro.ktgWsi*g1endihrfyaicvwaenetig. ht Table 2. Characteristics of the sediment used for testing Organic content {%) 0.7 Silt fraction (%) 0.8 LSaamb.prleef.rnecoe.:ived.: TReesptopretrdiaotde:: 007-21.900091..301.219202..990-9940 Enclosed: Datasheets Elin Horve AnalyEticnaglinteecehrnician SenQiAourgaRelieMtsyeoAalrvsceshrusrSmacnyiecrentist 002358 L99ab3.1r2e-f4.no.: Page 18 of31 PRIMARY DATA: Acute toxicity test with Corophium volutator Test substance: "FC-603 EF Light Water TM ATC TM 3%" Lab. ref.: Start date: Measurement: 99312-4 17.12.99 Number of dead Total number Number of specimen pr. vessel Concentration Specimen A C E (mg/kg dry weight) pr. cone. B D F Control 10 10 10 60 10 10 10 10 10 10 222 30 10 10 10 457 30 1060 10 10 10 30 3060 10 10 10 30 \ \ NUMBER OF DEAD 5 days 10 days ACE B DF Total ACE BDF Total 000 000 0 0 12 3 202 4 2 32 7 3 20 5 Reference substance (fluoranthene): 10 10 10 20.9 30 51 2 2 II 002359 L9a9b3.1r2e-f.4no.: Page J9 o f31 Test substance: Lab. ref.: Start date: "FC-603 EF Light Water TM ATC TM 3%" 99312-4 17.12.99 Measurements of oxygen and pH. Start Concentration Oxygen (mg/kg dry weight) (%) pH 5 days Oxygen (%) pH Control 94 8.1 90 8_J 222 96 8.1 92 8.1 457 97 8.1 93 8.1 1060 92 8.0 62 7.7 3060 100 8.1 93 8.0 10 days Oxygen (%) pH 97 8.1 94 8.0 97 8.1 96 8.1 96 8.1 20.9 95 8.1 92 8.1 83 8.0 002360 L99a 3b1.r2e-f4.n o .: Page 20 o f31 Data Sheets 002361 L99a3b1.r2e-f4.no.: Page 21 of31 HMS-DATA3LAD (Heise-, Miljo- og Sikkerhetsdatablad) Dokumentn r .: =.rsratter : 07-7297-0 Utgitt : 03/11/93 Versjon : 03.00 24/04/97 Skrevet ut : 11/11/98 Side 1 av ii HMS-databladet er utarbeidet av Miljaavdelingen ved 3M Norge A/S. Telefon: 63 84 75 00 / Telefax: 63 84 17 88 1 IDENTIC IXASJON AV KJSMIKALIET OG ANSVARLIG FIRMA". Importor: 3M Norge A/S Handelsr.avn: FC-603E? LIGHT WATER (TM) ATC 3% AR-AFFF Deklaras jor.snummer, Produktregisreret: Telefon, Giftinfonnasjonssentralen: 22 59 13 00 2 OPPLYSNINGER OM KJZMISK SAMMENSETNING. Informasjon om enkeltkomponenter, faresymbol(er) og risikosetninger STOFFNAVN CAS-nr VEXT-PROSENT VANN 1,2-ETANDIOL Faresymboler/risikosetninger: Xn; 2- (2-BCJTOKSYETOKSY)-STANOL / (DIETYLENGLYKOLMONOBUTYLETER) Faresymboler/risikosetninger: Xi; SYNTSTISK OVERFLATEAXTIVT STOFF FLUORALKYL OVERFLATEAKTIVE STOFFER FLUORKJEMISX AKRYLAT TRIETANOLAMIN TYKNERE RESTER AV ORGANISES FLUORKJE.MIKALIER 7732-18-5 107-21-1' 22 112-34-5 :36 Trade Secret Trade Secret Trade Secret 102-71-6 Trade Secret Trade Secret 52 - 56 - 22.5 - 10 4- 6 1- 5 1- 3 1 1- 3 Ikke kjent 002362 L99a3b1.r2e-j4.no.: tage 22 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF 3 VIKTIGSTS FAAEMOMENTER. Viktigste skadevirkninger Eelsefare: Kan vaere farlig ved svelging. Kan irritere aynene. Sikkerhet: Miljafare: Spesialavfall. I 4 F0RST! :JSL?STILTAX. Tiltak ayne: Skyll straks aynene med rikeLige mengder vann. Kontakt lege umiddelbart. Tiltak hud: Skyll straks huden med sipe og rikelige mengder vann. Fjern tilsalt tay. Evis symptomer oppstar, kor.takt lege. Vask tilsalt toy far det brukes igjen og kst tilsalt skotey. ..Ii j Tiltak innanding: Hvis symptomer oppstir, bring vedkommende bort fra eksponeringskilden. Sarg for frisk luft. Hvis symptomene vedvarer, kontakt lege. i Tiltak svelging: Kontakt lege umiddelbarn. Fremkall brekr.inger kun i samrid med medisinsk personell. Gi aldri noe gjennom munnen til en bevisstlas person. : j 5 TILTAK VED BRANNSLUKNING. -- Egnet brannslukningsmiddel: Produktet er et brannslukkingsraiddel. Ikke egnet brannslukningsmiddel: Eksponeringsrisiko ved brann: i ' 002363 L9a9b3.1r2e-f.4no.: Page 23 o/31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Forbrenningsprodukcer ved brann: Prosedyrer ved slukkir.g av brann: 6 TILTX VED UTILSIXTET UTSLIP?. Personlige vernetiltak: . Se vernetiltak under andre punkcer i dette HMS-databladet. Tiltak ved utilsiktet utslipp: Ventiler omr&det. Spill avgrenses umiddelbart. Dekk til med et absorberende materiale. Spill samles opp umiddelbart. Rengjer omridet med vann. Plasseres i en egnet beholder med lokk. Fjerning av kjemikalieavfall: Se punkt 13, Fjerning av kjemikalieavfall. 7 KANDTSRING OG 0PP3EVAING. I Betingelser for sikker oppbevaring: M ikke lagres i omrider hvor procuktet kan komme i kontakt med mat eller legemidier. Lagres ved temperatur under 49 grader celsius. Lagres ved temperaturer over 0 grader celsius. Hold beholderen tett lukkst n&r produkret ikke er i bruk. Uforenelige materialer: Ventilasjon: Oppbevar emballasjen p. et godt ventilert sted. kSikkerhetstiltak for forhindre brann: kSikkerhetstiltak for forhindre eksplosjon: Ikkebrennbar. kSikkerhetstiltak for forhindre statisk elektrisitet: Forholdsregler ved bruk: Hold emballasjen tett lukket. Reyking forbudt: Under bruk av produktet kan reyking forirsake forurensning av tobakken og/eller L99ab31.r2e-f4.no.: Page 24 o f31 FC-603EF LIGHT WATER (TM) ATC 3\ AR-AFFF 8 EKSPONERINGSXONTROLL OG PERSONLIG VERNEUTSTYR. t kOyevern: Unngi i damp, spray eller tike i aynene. Bruk tette vernebriller med ventiler. Hindvern: Bruk egnede vernehansker av hensiktsmessig materiale ved hindtering av produktet. Vernehansker av felgende materiale(r) anbefales: Butylgummi. Annet hudvern enn hindvern: Unngi hudkontakt. Andedrettsvern: * Unr.gi inninding av luftbiret materiale. Basert pi konsentrasjonen av luftforurensninger i arbeidsatmosfaren velges en av folgende type{r) godkjent incedrettsvern: halvmaske med filter mot organiske damper og vaskeformige partikler: A/P2SL. Svelging: Det mi ikke spises, drikkes eller ravkes under arbeid med produktet. Vask utsatte omricer grundig med sipe og vann. Vask hendene etter hindtering og far spising. Anbefalt ventilasjon: Hvis utsugningsvdntilasjon ikke er tilstrekkelig, mi egnet godkjent indedrettsvern ber.yttes. Sarg for tilstrekkelig ventilasjon slik at dampkor.sentrasjonene holdes under anbefalte administrative normer og/eller kontrolier spray eller tike. Adm. normer for foruser.sning i arteidsatmosfare, Februar 1996: VANN {7732-13-5) Anmerkninger-: Ingen adm. norm. 1,2-ETANDIOL (107-21-1) 8-timers verdi ppm: - (stov) 8-timers verdi mg/m3: 10 (stav) Takverdi ppm: 25 (damp) Takverdi mg/m3: - (damp) itI 2-(2-BUTOKSYETOKSY)-ETANOL / (DISTYLENGLYKOLMONOBUTYLETER) (112-34-5) 8-timers verdi ppm: 35 Anmerkninger: Amerikansk grenseverdi anbefalt av produsenten. (Ingen norsk adm. norm). SYNTETISK OVERFLATEAKTIVT STOFF (Trade Secret) Anmerkninger: Ingen adm. norm. -.j. 002365 L9a9b3.1r2e-f.4no.: Page 25 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF - ?SrH0RKJ5-t-3X AI-tyiLAT {Trade Secret)------ -----------------Anmerkninger: Ingen adm. norm. TRIETANOLAMIN (102-71-6) 8-timers verdi ppm: 8-timers verdi mg/m3: 5 TYXNERE (Trade Secret) Anmerkninger: Ingen adm. norm. RESTER AV ORGANISKS FLUORXJSMIKALIER (Trade Secret) 8-timers verdi mg/m3: 0,1 Grenseverdi anbefalt av 3M. iI 1 9 FYSISXE OG XJEMISXS EGENSXAPSR. Tilstandsform, farge, lukt: Gul/brun vsske. ! pH: 7.5 - 8.S - Xokepunkt/-intervall: Smeitepunkt/-intervall: < 150 C Ikke relevant i ; Flammepunkt: Ingen (Setaflash) Selvantennelighet: Ikke kjent Sksplosjonsgrenser, nedre: Ikke relevant Eksplosjonsgrenser, ovre: Ikke relevant 1 Damptrykk: ca. 15.8 mmHg (Kalkulert v/2QC) ; Laselighet i vann-: Spesifikk vekt: Fullstendig * ca. 1.06 (vann=*l) Damptetthet: ca. 0.88 (luft*l) (Kalkulert V/20C) ; ; i Flyktige organiske forbindeiser (VOC) - 355 g/1 SCAQMD v/20C). (Kalkulert | i Fordampningshastighet: Ikke kjent Viskositet: < 5000 centipoise - := : 002366 L9a9b3.1r2e-f4.no.: Page 26 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF IO STA3ILITET OG REAXTIVITET. Forhold som skal unngis: Materialer som skal unngis: Ikke kjent. Farlige spaltningsprocukter: Karbonmonoksid og karbondioksid. Hydrogenfluorid. Giftige damper, kgasser eller partikler. Dekomponering av bruksferdig blanding forventes ikke representere noen risiko. Stabilitet og reaktivitet: Stabilt. Farlig polymerisasjon vii ikke forekomme. j 1f ! 11 OPPLYSNINGER OM EEL5EFARS. Effekrer ayne: Mild eyeirritasjon: symptomer kan vare redhet, hevelse, smerter og tirer. j Effekter hud: Mild hudirritasjon .(etter langvarig eller gjentatt kontakt): symptomer kan vare redhet, hevelse og klee. Langvarig eller gjentatt eksponering kan fere til: Allercisk hudreaksjon: symptomer kan vare redhet, hevelse, blemmer og klee. Effekter inninding: En enkelt overeksponering som overstiger anbefalte administrative normer, kan fere til: Pvirkning av ser.tralnervesystemet: symptomer kan vare hodepine, erhet, sevnighet, manglende koordinasjonsevne, nedsatt reaksjonsevne, sleret tale, svimmelhet og i ekstreme tilfeller bevisstleshet. Irritasjon (evre luftveier): symptomer kan vare srhet i nese og svelg, hosting og nysing. Effekter svelging: Dyreforsek utfert med organiske fluorkjemikalier, som finns i s dette produktet, viser effekter som leverforstyrrelser, vekttap, manglende appetitt, slevhet samt effekter som pvirker neurologi, C02367 bukspytt, binyre og hematologi. Oet forelicawr f " " v -*-- L9a9b3.1r2e-f4.no.: Page 27 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF veiledning. Svelging kan fare til: Pivirkning av nyrene: symptomer kan vare redusert urinmengde, bld i urinen og rvggsmerter. Aspirasjonspneumoni (Lungebetennelse som skyldes at fremmedlegemer kommer ned i lungene. En alminnelig rsak til tilstanden er oppkast.): svmpromer kan vare hosting, pustevansker, gisping, hoste opp bld og lungebetennelse som kan vare livstruende. Informasjon on allergi, overfolsomhet: Xreftfremkaliende egenskaper: Arvestoffskadelige egenskaper: Reproduksjonsskadelige egenskaper: Andre skadevirkninger, helse: Dente Produkten ir.neholder en eller fiere organiske fuorkjemikaier, som muligens kan absorberes og lagres i kroppen over lengre tid, enter, som utgangsmolekyiet eller som necbrytningsstoffer (metabolitter), og kan akkumuleres ved gjentatte eksponeringer. Det foreligger ingen kjente helseeffekter for mennesker fraeksponering overfor organiske fuorkjemikaier nr de brukes som forutsatt og i benhold til veiledning. Forakomsnen av organiske fuorkjemikaier i blodet til den generelle befoikningen og underpopulasjoner som arbeidere, har vart publisern siden 1970 irene. 3Ms epidemiologiske studier av egne arbeidere viser ingen skadelige effekter. 12 OPPLYSNINGER OM MILJ0FARE. Persistens/aiologisk nedbrytbarhet: Dette stoffet er ikke lett biologisk nedbrytbart, men brytes delvis ned i tester for lett biologisk nedbrytbarhet. :rr^r" Dette produktet inneholder en eller fiere organiske fuorkjemikaier som muligens ikke er nedbrytbare og forblir i miljoet (persistente). Okotoksikologiske data: I Dmect/t1e<stlaovfof<est*aetrrmninimrarlt nt gi. fnt.i.g ovecrfo' r - a1k' v--ati- sk' e org--an''is' mer (100 0 0 2 3 6 8 L9a9b3.1r2e-f.4no.: Page 28 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF Andre skacevirkninger, miljo: Data on biologisk r.edbrytbarhet: COD (Chemical Oxygen Demand): 740000 mg/kg; BOD 5 dagers (Biochemical Oxygen Demand): 216000 mg/kg; 30D 10 dagers: 376000 mg/kg; BOD 20 dagers: 446000 mg/kg; BOD 28 dagers: 602000 mg/kg. Akvatisk giftighet: 96 timers EC50 for fisk (Fathead Minnow (Pimephales promelas)): > 1000 mg/1; 48 timers EC50 daphnia (Water Flea (Daphnia_-magna)): 480 mg/1; 30 mir.utters EC50 Microtox: 124 mg/1;24 timers-EC50 Alge (Selenastrum capricornutum)): 170 mg/1. Behandling i renseanlegg: 3 timers SC50, Activated Sludge Respiration Inhibition: > 1000 mg/1. Sorg for avfallshinctering iht. gjeldende fcrskrifter. 13 FJERKING AV KJEMIKALIEAVFALL. Metoaer for fjerning av kjemikalieavfall: Spesialavfall. Lever avfall til en offentlig godkjent innsamler, mottaks- eller behandlingsanlegg for spesialavfall. Anbefalte avfallsgrupper: HOVSDKATSGORI 07: AVFALL FRA ORGANISES PROSES5SR. UNDERRATEGORI 07 06: Avfall fra P3DB av fettstoffer, s&pe, rengjoringsmidler, desinfeks jonsmidler og kosaetikk. EAK-kode 07 06 03: Halogenerte organiske lesemidler, vaskevasker og moaerlut. Avfallsroffnummer: 7151 Organisk avfall med halogen. Spesielle komponenter: inneholder fluorforbindelser. 14 OPPLYSNINGER OM TRANSPORT. ADR-klasse: siffer: 00 L99a3b1.r2e-f4.no.: Page 29 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF bokstav: margnummer: CEFIC-kort: UW-nummer: ICAO-klasse: IMDG-klasse: IMDG-sice: EMS-r.r.: MFA G : Ovrige oppiysninger: Ikka transportfarlig gods. 15 OPPLYSNINGER OM LOVER OG FORSKRIFTER. Merkeetikett: Symbol(er): EELSESXAOELIG (Xn) laneholder: Etylenglykoi 22,5 %, 2-(2-butoksyetoksy)-etanol 10 %, Trietanolamin 1% R-setninger: Farlig ved sveiging. S-setninger: Unng inn&nding av damp/spreytetike. angi kontakt med huden og aynene- Fir man stoffet i aynene; skyll straks grundig med stre mengder vann og kontakt lege. Fir man Stoff pi huden, vaskes straks med stre mengder sipe og vann. Ved svelging, kontakt lege omgiende og vis denne beholderen eller etiketten. YL-gr0u0ppe: Korr, yrkeshyg. luftbehov: Ovrige oppiysninger om merkeetikettens _. Ovennevnte merking er basert pi tidligere merkeforskrifter og vil 00(2370 L99a3b1.r2e-f4.no.: Page 30 of31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF revider"e merkeforskrifter av januar 1993. Symbol: Ingen Inneholder: 1,2-etandiol, 2-(2-butoksyetoksy)-etanol, Triecar.olarain Xan vare farlig ved svelging. Kan irritere oynene. Unngi innir.ding av dartp/sproytetke. Unngi kontakt med huden og oynene. Fir man stoffet i oynene; skyll straks grundig med store mengder vanr. og kontakt lege. Fir man Stoff pi huden, vaskes straks med store mengder sipe og var.n. Ved svelging, kontakt lege pmgiende og vis denne beholderen eiler etiketten. Y00L-gruppe: Spesielle lover og bestemmelser: 1. Forskrift om utarbeidelse og distribusjon av Kelse-, Miljo- og Sikkerhetsdatablad for farlige kjemikalier. 2. Forskrift om klassifisering og merking av farlige kjemikalier. 3. Forskrift om klassifisering, merking m.v. av stoff som kan mediare miljofare. 4. Forskrift om stoffkartotek. 5. Forskrift om spesialavfall. 6'. Etc. 16 ANDRE OPPLYSNINGER AV 3ETYDNING FOR 3RUKSRNSS SIKXSRHET OG HELSE. Anvendelsesomrider: Filmdannende skumkonsentrat for slukking av brann. Viktigste kilde(r) ved utarbeidelsen av HMS-databladet: Datablad fra- 3M General Offices, St. Paul, Minnesota, USA. rsak til oppdatering: Hele databladet er revidert. Tilpasset nye merke- og HMS-databladforskrifter, fastsatt i januar 1998. Etc. Egne opplysninger: Informasjonen pi dette HMS-databladet er i henhold til vire nvrence opplysninger og beste oppfatning angiende riktig bruk og hindteringen av dette produktet under normale forhold. Enhver bruk av produktet som ikke er i samsvar med dette HMS-databladet, eller som medforer bruk av produktet i kombinasjon med andre produkter eller enhver annen prosess er brukerens ansvar. 0023tl rum L9a9b3.1r2e-f4.no.: Page 31 o f31 FC-603EF LIGHT WATER (TM) ATC 3% AR-AFFF FOLGENDE PRODUKTER DEXXSS AV DETTE HMS-DATABLADET: 3M ID-ar : XF-0008-0223-1 3M AFFF/ATC FC-603EF Brannslukn ingsskum Produksjon 3M ID-nr : XF-0008-0209-0 3M Light Water3/3 %AFFF/ATC Fire Fighting Foam, FC-603EF,1000 L 3M ID-nr : XF-0008-0208-2 3M Light Water3/3%AFFF/ATC Fire Fighting Foam, FC-603EF, 200 L 3M ID-nr : XF-0008-0207-4 3M Light Water3/3%AFFF/ATC Fire Fighting Foam, FC-603EF, 20 L 3 002372