Document 6Rz3LMj23QRnJKpRLGg05wLVR

FOR DU PONT USE ONLY E. I, du ft>nt de Nemours and Co., Inc. Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P. 0. Box 50, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 793-81 Haskell No. 3,-- Studies Inltiated/Completed " 1 0 / 2 / ^ 8 0 - 1 1 / 2 1 / 8 ----- 1/5/81 - 1/30/81 Material Submitted bv Iblymer Products Department Washington Works Parkersburg, WV Summary: SUBACUTE INHALATION TOXICITY STUDY "TrTS ^ a r a t ^ x p e r l m e n t s , male and female CrlrCD* rats were exposed to each study, groups of 10 rats were exposed to design concentrations of either 0, 500, 5000, or 50,000 ppm for periods of 6 hours/day, 5 days/week for 2 weeks. The actual test concentrations for the aalej exposures were 541, 4750, and 46,300 ppm; female exposures were 473, 5140 arid 46,800 ppm. PP^ renale Clinical observations of test rats during exposure were indistinguishable from those of controls. Male and female rats In all test groups showed a normal rate of weight gain throughout the study. Clinical chemistry measurements on rats exposed to 500 ppm showed no compound-related changes, following 10 exposures male rats exposed to 50,000 ppm tended to have significantly lower platelet counts and to excrete more fluoride in the urine than the controls. Female rats exposed to 50,000 ppm tended to excrete more fluoride and urobilinogen in the urine than the controls. Females exposed to 5000 and 50,000 ppm had significantly more noeytes in the blood than: the controls. No compound-related changes were observed 14 days post-exposure. 8 Pathologic examinatii showed no compound-related macroscopic or microscopic changes in any tats exposed to - 1- Company Sanitized. Does not contain TSCA CB! * cr ^ : z ^ : ( s z z s . ' s `r -- > < . M ii8S i l c M t l y hlgher than controls i ^ b o X * T * ' i 1*"7 wel*ht# were 50 000 ppm. Liver weights were s i g n i f i c M C l ^ h i e X / X * 16 rat" e*po8ed to rats exposed to 50.000 ppm. Fourteen lif-II X f" COntro18 in male effects were still aeen in male rat y> f5? a * these liver and kidney mmii1cTMrosc1o"pi?c6 6 ,lc^.lotMh e M ,,e f foebc.t os cvloid ilnf f l c u l t 1 o 8l bt8 l d e d tD f 6 m a l e S - ^ _I. Introductions -- mmSSmmSmSl^T S d famaia ^ to determine the effects " W t a f t e r r X e f CheSe studies was to concentrations were 0 ppm (Group I) son inhal-a-t-i-o-n e--xposure. uDesxign and 50,000 ppm (Group IV) P ' ' v.QGrr0o1u1p0 ITIT)', *5n00n0n ppffl (Gtou; i n ) 11 Procedure; A. Animal: Male and female rvi .nne> x 14" stainless steel canes and m- *a a ratS 16,61:6 housed 2/cage in 8" x 8" w " =-r" osy (,1 lihl"g 230 only. All rats were w i s h e d a n d ^ b s f r i e V d l i l v 1^ CanUSl,y exposed toair exposure period and for 14 days post-exposuie^7 ( pt Weekends) through the liq"ld a SprayingfSystems neb*!!66*1 ^ SJrria*6 driving the surface (30'C) of a rouid-Stfo7^ fi X * nt the heated vapors into a 20 L glass exposure c h ^ U r ^ * lr carrled the resulting D. Analytical * flHBli. 'ii1" . n - C * L S S t S ^ i S e S r * HS eccti R,c," ri. 5710 085 a L r s s s i . " & - w/" s r < i 2 J 5 ` n s a df,iuci" f lacerv^a. Standards and sasipLi m e . la...!1,' **r collected at half-hour syringe. Atmospheric conceritratioX were^alXl!? !? *he 00 by &atight are e i p r M M d - - - n c . c t r . t i o i ' i o t " 1: ; =TM Company Sanitized. Does not contain TSCA CB1 2 12 Clinigal Measurements * : Clinical laboratory measurements were made on urine samples collected overnight following the 9th exposure and the 13th day of recovery. Analysis Included a quantitative measure of the volume, osmolality and pH, and semi-quantitative tests for occult blood, protein, sugar, bilirubin, acetone, and urobilinogen. Each specimen was noted for color and appearance and the sediment from pooled specimens examined microscopically. The concentration of fluoride and total fluoride were also measured. Blood samples were taken from the rats tails after the 10th exposure and the 14th day of recovery for the measurement of the following: erythrocytes, hemoglobin, mean corpuscular volume, platelets, leukocytes and relative number of neutrophils, lymphocytes, eosinophils, monocytes and basophils. The-- hemat^CTi-t^mean wr pu se ui aT hemoglobin and"mean"corpuscular hemoglobin concentration wete calculated from these data. Alkaline phosphatase, glutamic-pyruvic transaminase, glutamic-oxaloacetic transaminase, urea nitrogen, creatinine, total protein and cholesterol were also measured. F. Pathology3 : After the 10th exposure, 5 rats from each group were selected at random and sacrificed for gross and histopathological examination. Remaining rats were sacrificed on the 14th day of recovery for identical examinations. Organs and tissues examined included the following: liver, kidneys, heart, spleen, bw.se marrow, thymus, brain, eyes, skin, ear, adrenals, pancreas, lungs, trachea, thyroid, parathyroid, lymph node, esophagus, stomach, small ibtestines, colon, and cecum. Also examined were testes and epididymides in males; ovaries, uterus, bladder, and vagina in G* gggan and Body Weight Analysis: At each sacrifice organ weights and organ to body weight ratios were determined for the heart, liver, lungs, kidneys, spleen, testes (males), ovaries (females) and thymus. These data are detailed in Appendices I and II. ' "Subacute Inhalation of fi ni ca l Laboratory Report, H-13,783, January 16, 1981. Walter^B^Koniecki and John R. Barnes, "Subacute Inhalation of MmHMI >H-13,783, March 20, 1981. Clinical Laboratory Report, Edwin F. Stula and William C. Krauss, "Pathology Report No. 19-81" H-13,783, June 17, 1981. ' .Company Sanitized. Does not contain TSCA CB1 3 J i -- I .. ? 5= I > 5* Islllilll! I ??S7S5SS? : 5 IHlIliili ? ; = ig ;: sfiiiiiin ; fiiiiiiiii 32;i5Ss;4 i I 5 h- i:i-i; = -:j. ^ IT : i * :j '' I| : I I 1 I I I I I I I * S?S55*;;?5 I S j t???ili*lf 1N, P IMN IJIIIIIIII i'l,IllfillllS J :J_ -ir * 5S = ;s?: Is! =!s Ji " !**s3*SS55 si * 1 i ISfIslllll i ii ! ? ? s ? * m s * n|-j3 ;! j S= iS iS iS ji a3i?l9U5oa - J ^ v C <0r-irt i: ; - 5 = 5 s' s s s s i. i * f i 5i2S355S13 I i r= ^*= ( 111 1 SSSSlSfslI s lllillllll ssSsIssill =Sg=55=SS IIs 2\i i.1'if!s ;' = 1 s;|S:ssss; I Itro e ^ Jr Sanitized- Does not contain TSCA CB1 Company r B \ C1.inlcal Observations: During exposure, clinical observations of test rats were indistinguishable from those of controls. C. C U n l c a l Chemistry: Clinical chemistry measurements on Croup II IV ratstfndpd compound-related changes. Following 10 exposures, male Group J tended to have significantly lower platelet counts and to excrete ? more fluoride in the urine than the controls. Female Group IV rats tended to excrete more fluoride and urobilinogen in the urine than the controls. Groun thhee ccoonnttrroollss.1* XNofecmoamlpeosun]d?a-drellagtendlficchaanntgleys wDeorree omobnseorcvyetdes 1i4ndatyhse blood than post-exposure. 7 D. Ethology: No compound-related macroscopic or microscopic changes were observed-in any of the rats exposed P cnanges ,, E * ,,-- 5 and 0rgan Wel*ht Analysis: Male and female rats in all test fndUS) S i* norinai of welght gain throughout the study (Figures 1 and 2). Female rats in Group IV had significantly lower body w e i g h t than ooffntihhee1itepsftrralnndf ifshenoetxp0c8ounrseidePreerdiode,xpohsouwreev-erre,lattheids. was so prior to the start A comparison of organ to body weight ratios between exnosed rats and eontrois showed a few differences. Following the 10th exposure, kidney IV 3lgnifiCantly hl*hp than controls in both male and f e S l e Group ii r a ^ 'oniver !ight Were s ^ i c a n t l y higher than controls in male &oup aftlr dl y ` uTi*!IeJ11V! r and kldney effects were still evident in males if d < % d?lS bu, h8d 8ub8ided in fenales. The significance of these effects difficult to interpret since no specific microscopic lesions were observed in any of the rats exposed to M B Company Sanitized. Does not contain TSCA CBI 5 Work by: B o d e r 7- 1 '' " 'i Rocere T. Turned Technician oy ; --- ^yanne l7~FeraS Toxicologist Study Director Reviewed by: J M i f f y tfjj Bruce' A^ ur ge ii--Research Ti6/icicologist Approved by: HIT;jrg:WP:ls.i4 Date Report No. 793-81 1 2 . 19a? JZLuhLA. ^irTTSSfcV Section Supervisor, Acute Investigations Company Sanitized. Does not contain TSCA CBI 6- FIGURE 1 w TEST BAYS - 7^ t1 I I Company Sanitized. Does not contain TSCA CBI FIGURE 2 a9 HE W l l I f PAr.MAMO SAVOISU II e s Company Sanitized. Does not contain TSOA C 31 APPENDIX I BODY WEIGHT SUMMARY I Company Sanitized. Does not contain a oni -9 (HALE) - BODY HEIGHT SUMMARY CROUP INTERVALS IN TEST CAYS 1. 3. CONTROLS LOU (SOO PPM) INT. <3.000 PPM) HI8H <30.000 PPM) F RATIO!1> LSD<2) DUNNETT<3) U N S (4 > 249.3000 242.3000 249.0000 248.2000 0.873 9.8247 11.9342 117.1167 34.3000 249.,0000 233.3000 230.7000 0.386 11.6802 14.2119 163.3306 262.3000 233.4000 239.2000 233.3000 1.101 12.1913 14.8339 180.3389 4. 263.6000 237.2000 263.3000 258.6000 0.900 12.0293 14.6369 173.3806 3. 283.2000 277.4000 282.8000 277.3000 0.808 12.6372 13.4006 194.3806 GROUP INTERVALS IN TEST DAYS a. 9. 10. CONTROLS LOU (300 PPM) I M T . i3.000 PPM) HIGH <30.000 PPM) 293.7000 2B4.BOOO '289.8000 237.0000 F RATIO(l) LSB(2) DUNNETT(3 > UNS <4) 0.609 14.1238 17.1831 242.0361 299.8000 290.7000 296.3000 290.3000 0.763 14.9761 18.2222 272.1306 303.9000 293.1000 300.4000 293.9000 0.692 14.1802 17.2338 243.9750 11. 109.4000 297.1000 303.7000 299.0000 1.206 13.0118 18.2656 273.4278 12. 303.0000 294.5000 304.7000 296.9000 0.937 13.9331 19.3890 308.0972 GROUP INTERVALS IN TEST Dar'i 15. 16. :- CONTROLS LOU (SOO PPM) INT. (3.000 PPN) HIGH <30.000 PN> F RATIO!1) LSD< 2> DUNNETT!3) UNS <4 > 336.6000 317.4000 322.6000 314.2000 1.492 24.2828 30.1238 328.0230 339.6000 319.6000 326.0000 316.8000 1.297 26.7418 33.1766 397.9230 345.2000 325.4000 334.8000 323.2000 1.341 26.0187 3 2 .2 7 9 h 376.4000 18. I S . ..050 331.0000 338.4000 330.2000 1.034 :' .3897 34.2284 423.4500 19. 356.8000 338.>000 346.0000 334.0000 1.229 26.9986 33.4931 >03.5000 Company Sanitized. Doe3 not contain TSCA CB1 I GROUP INTERVALS IN TEST DAYS 22. 23. 24. CONTROLS LOU :300 PPM) INT. 5.000 PPH) HIGH (50.000 PPH) F RATIO!1) LSD(2> DUNNETT!3) W H S (4) 370.2000 330.0000 361.8000 330.0000 1.087 27.7262 34.3977 427.6500 376.2000 336.4000 368.8000 337.0000 0.935 29.8005 36.9812 494.3000 384.4000 364.1000 374.aOOO 362.6000 ' .078 29.0101 35.9907 468.1750 2t. 389.6000 368.2000 378.0000 367.6000 1.012 30.7992 38.2102 527.7000 26. 376.4000 362. 0 370.8000 336.6000 1.0S2 25.759? 3 1 . 9 5 'f 369.125: <1) RATIO OF ANONO- TO WITHIN-GROUP VARIATION-- ONE-FACTOR ANALYSIS OF VARIANCE. (2) LEAST SIGNIFICANT DIFFERENCE-- GIVEN A SIGNIFICANT (ALPHA-0.03) F RATIO. ANY TWO MEANS DIFFERING BY MORE THAN THE LSD ARE S IGNIFICANT DIFFERENT HITH A VARIABLE-HISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. <3> DUNNETT TEST-- ANY TREATMENT MEAN DIFFERING FROH THE CONTROL MEAN BY MORE THAN THE DUNNETT STATISTIC IS SIGNIFICANTLY DIFFERENT FROH THE CONTROL MEAN WITH A VARIABLE-WISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. (4) WITHIN-QROUP MEAN SQUARE. + SIGNIFICANTLY DIFFERENT (P<0.03) FROH CONTROL GROUP BY LSD. # SIGNIFICANTLY DIFFERENT <P<0.05: FROH CONTROL GROUP BY DUNNETT TEST AND LSD. Company Sanitized. Does not contain TSCA CBI (FEMALE) - BODY WEIGHT SUMMARY GROUP INTERVALS IN TEST DAYS 1. 2. 3. 4. 3. CONTROLS LOU (300 PPM) INT. (3.000 PPM) HIGH (30.000 PPM) F RATIO(l) LSD(2) DUNNETT(3) U M S ( 4) 197.1000 191.1000 190.4000 184.40000 2.437 9.3102 11.3716 109.7389 2*0.1000 194.8000 193.2000 188.3000t 1.972 9.7221 11.8294 114.6833 203.7000 197.8000 199.2000 189.9000 2.928* 10.8839 13.2434 143.7933 207.7000 198.4000 200.1000 192.7000# 2.723 10.7646 13.0979 140.3972 210.0000 243.3000 203.1000 194.6000 3.102* 10.4713 12.7409 133.0389 GROUP INTERVALS IN TEST DAYS 8. 9. 10. 11. 12. CONTROLS LOU (300 PPN) INT. (3.000 PPN) HIGH (30.000 PPM) F RATIO!1) LSD(2) DUNNETT(3) UM8(4 > 220.3000 214.4000 212.9000 20S.6000t 1.946 12.4439 13.1411 187.8833 220.7000 213.4000 214.8000 206.2000 2.341 11.2396 13.7002 133.6230 226.8000 219.3000 219.3000 211.3000* 2.604 11.1129 13.3216 149.8417 227.2000 222.0000 222.2000 212.3000 2.493 11.3282 13.7833 153.7028 '26.7000 .2.7000 222.3000 213.3000t 1.771 12.0083 14.6113 t 7 4 .9667 GROUP INTERVALS IN TEST DATS 13. 16. 17. CONTROLS LOU (300 PPN) INT. (3.000 PPM) HIGH (30.000 PP> F RATlO(l) LSD(2) 9UNNETT(3) WHS(4 > 240.6000 232.8000 239.6000 228.2000 1.381 14.9673 18.3690 124.6250 240.4000 226.8000 242.0000 228.0000 2.662 14.7177 18.2391 120.5000 243.0000 234.4000 239.4000 231.8000 0.773 17.1017 21.2163 162.7000 18. 245.2000 236.6000 243.4000 236.8000 0.69S 15. 979 14.8474 142.3750 19. 248.0000 240.4000 246.4000 239.2000 0.780 14.7630 13.3215 '.21.3250 .Company Sanitized. Does not contain TSCA CBI GROUP INTERVALE IN TEST DAYS 23. 24. CONTROLS LOU (SOO PPM) INT. <5000 PPM) HIGH (SOtOOO PPM) F RATIO(1> LSD<2) DUNNETT(3) W M S (4 > 260.2000 248.2000 236.2000 230.6000 0.746 18.8290 23.3397 197.2230 233.6000 230.8000 239.0000 230.8000 0.473 17.4000 21.3868 168.4230 237.8000 230.8000 261.2000 248.2000 0.892 19.1473 23.7346 203.9300 23. 261.2900 232.0000 263.2000 234.4000 0.706 21.6987 26.9199 261.9230 24. 261.6000 234.8000 263.4000 237.2000 0.484 20.2670 23.1437 228.3000 (1) RATIO OF AMONG- TO UITHIN-GROUP VARIATION-- ONE-FACTOR ANALYSIS OF VARIANCE. (2) LEAST SIGNIFICANT DIFFERENCE-- GIVEN A SIGNIFICANT (ALPHAaO.OS) F RATIO* ANY TWO MEANS DIFFERING BY MORE THAN THE LSD ARE SIQNIFICATLY DIFFERENT WITH A VARIABLE-WISE FALSE POSITIVE (ALPHA) ERROR RATE OF O.OS. (3) DUNNETT TEST-- ANY TF 4TMENT MEAN DIFFERING FROM THE CONTROL MEAN BY MORE THAN THE DUNNETT STATISTIC IS SIGNIFICANTLY DIFFERENT FROM THE CONTROL MEAN WITH A VARIABLE-WISE FALSE POSITIVE (ALPHA) ERROR RA E OF O.OS. !4' WITHIN-GROUP MEAN SQUARE. SIGNIFICANTLY DIFFERENT CP-TO.OS) FROM CONTROL GROUP BY LSD. SIGNIFICANTLY DIFFERENT <P<0.03> FROM CONTROL GROUP BY DUNNETT TEST AND LSD. Company Sanitized. Does not contain TSCA CB1 13 - APPENDIX II ORGAN & BODY WEIGHT DATA Company Sanitized. Does not contain TSCA CB! 1-4 (HALE) - ORGAN 1 BODY HEIGHT HEAM ABSOLUTE D A T A RATS SACRIFICED AFTER 10 EXPOSURES GROUP FINAL HOT. HEART LUNGS LIUER CONTROLS LOU (900 PPH) INT. <3.000 PPM) HIGH (50.000 PPH) F RAT10(1> LSD<2> D U N N E T T (3) UNS(4> 299.4000 292.8000 303.8000 295.4000 .456 20.9699 26.0157 244.6250 1.0100 .9400 1.0760 1.0920 2.517 .1312 .1628 .0096 1.9280 1.8320 1.8240 1.7360 .603 .3031 .3761 .0511 10.8380 10.7260 11.9740 13.22204 4.046a 1.7388 2.1572 1.6819 SPLEEN .6940 .5940 .6480 .6360 .900 .1443 .1790 .0116 GROUP KIDNEY CONTROLS LOU (500 PPH) INT. (5000 PPH) HIGH (50.000 PPH) F RA T I O (1> LSD<2) DUNNETT<3) UHS<4) - 2.2520 2.2420 2.3760 2.6820 3.3811 .3347 .4152 .0623 TESTIS 2.9360 3.1300 2.8580 2.9940 1.401 .2907 .3606 .0470 THYHUS .6660 .7600 .7520 .6960 .775 .1536 .1906 .0131 (1> Ratio of aoono- to within-arouP variation-- ona-factor analwsis of varianca. (2> Laaat sianificant difference-- aIvan a significant (alPha0.05) F ratio, anv two ooana differind bw aora than tho LSD ara sianificantlw different with a variable-wise falsa positive (alpha) arror rata of 0.05. <3> Dunnatt tast-- Anw traataant aaan diffarins froa tha con'.rol aaan bw nor* than tha Dunnatt statistic is sianifieantlw diffarantfroa t.ia control aaan with a variable-wise falsa positive (alpha) arror rate of 0.05. (4) Uithin-eroup Mean Souare. + Sianificantlw different <P<0.05) froa control srour bv LSD. Sianificantlw diffarant (P<0.05> froa control aroup bw Dunnatt tast and LSD. s Sianifleant at tha 0.05 probabilltv level. (HALE) ORGAN 1 BOOT HEIGHT MEAN RELATIVE DATA R A T S SACRIF I C E D AFTER 10 EXPOS U R E S QR0UP CONTROLS LOW (500 PPH) INT. (3.000 PPH) HIGH (30.000 PPH) F RATIO(l) LSD(2) BNHETK3) U H S (4) HEART .3391 .3213 .3343 .3493 .484 .7391 ..>483 .0009 LUNGS .4434 .4294 .4007 .5875 .587 .1034 .1283 .0040 LIVER 1.4314 3.4404 3.9419 4.44204 7.108 .4330 .3372 .1043 SPLEEN .2313 .2030 .2131 .2319 1.025 .0422 .0324 .0010 KIDNEY .7321 .7438 ,7924 .90744 3.744 .0893 .1108 .0044 GROUP TESTIS THYHU8 CONTROLS LOU (500 PPH) INT. (5.000 PPH) HIGH (50.000 PPH) F RATIO(l) L S D (2) DUNNCTT<3> UH8< 4) .9833 1.0749 .9413 1.0134 1.787 .1273 .1379 .0090 .2234 .2379 .2472 .2333 1,104 .0424 .0328 .0010 <1> Ratio of aeong- to *lthin-grouP variation-- ono-factor analvsis of variance. (2) Laast significant difference-- aivgn a significant (alrha0.03> F ratio, anw two aoans Oiffarina be ore than tha LSD r# sianificantl* different with a verleble-wise falsa positive (alpha) error rate of 0.03. <35 Dunnatt test-- An* traataant aaan Oiffarina froa tha control naan b* acre than the Dunnatt statistic is sianificantl* different froa the control aaan *ith a veriable-utsa falsa positive (alpha) error rata of 0.03. (4) Hithin-grouP Haan Sauara. + Sianificantl* different <P<0.05) froa control group b* LSD. * Sianificantl* different <P<0.03) froa control srour b* Dunnatt test and LSD. t Significant at tha 0.03 probability level. Company Sanitized. Does not contain TSCA CBI (HALE) - ORGAN t BODY HEIGHT MEAN ABSOLUTE DATA RATS SACRIFICED AFTER 14 -DAY OBSERVATION GROUP FINAL WOT. HEART LUNGS LIVER CON.ROLS LOU (300 PPH) INT. (5.000 PPH) HIGH (30.000 PPH) F RATIO!1) LSD(2> DUNHETT(3) UHS(4> 376.4000 362.2000 370.8000 336.6000 1.032 23.7392 31.9575 369.1250 1.2140 1.1420 1 .ISSO 1.1720 .420 .1420 .1772 .0113 1.0440 1.9080 2.1440 1.9230 2.467 .2093 .2397 .0244 14.30GO 13.0680 15.2000 15.2600 2.29G 2.0238 2.5133 2.2831 SPLEEN .6580 .7220 .7020 .6720 ,434 .1316 .1633 .0096 GROUP KIDNEY TESTIS THYHUS CONTROLS LOU (300 PPH) INT. <3*000 PPH) HIGH (30*000 PPH) F RATIO!1) LSD(2> D U N N E T T (3) U N S (4) 2.7760 2.3740 2.9380 3.0220 2.495 .3747 .4649 .0781 3.3140 3.2940 3.2720 3.1740 .288 .3470 .4304 .0670 .7760 .7340 .8060 .8660 .553 .1938 .2429 .0213 (1) Ratio of aona- to uithin-aroup variation-- ona-faetor analasis of variance. (2) Laaat sianiflcant difference-- "Siven a sianiflcant (alpha"0.05) F ratio. ana tuo aan difforins ba ora than tha LSD ara sianifeantla diffarant with a voriablo-wisa falsa positiva (alpha) arro ? rata of 0.05. (3) Dunnatt test-- "Ana traataant an diffarina fro th control ean ba ore thar, th Dunnatt statistic is sianificantlv dlffarant fro tha control naan Kith a varlabla-wlse fai* coattiva(alpha) arror rata of 0.03. (4) Uithin-aroup Naan Souara. + Sianificantla diffrant <P<0.03> fro control aroup be LSD. Sianifleantle dlffarant (P'O.OS) fro control aroup be Dunnatt test and LSD* - 17 - Company Sanitized. Does not eonfgin T8S^ <3ff (MALE) - ORGAN t BODY WEIGHT MEAN RELATIVE 3ATh RATS SACRIFICED AFTER 14-DA.' OBSERVATION GROUP HEART LUNGS LIVER SPLEEN CONTROLS LOU (300 PPM) INT. (3.000 PPM) HIGH (50.000 PPM) F RATIO(l) LSD(2> 0UNNETT3) UM3(4) .3234 .3154 .3110 .3280 .473 .0334 .0413 .0000 .3179 .3278 .57824.3400 2.125 .0343 .0073 .0010 3.A09S 3.0002 4.0909 4.2713+ 3.719 .4002 .3709 .1178 .1731 .1991 .1393 1897 .738 .0340 .0421 .0000 KIDNEY .7401 .7123 .7908 .3433+ 3.143 .1013 .1237 .0037 GROUP CONTROLS LOU (300 PPM) INT. (3.000 PPM) HIGH (30.000 PPM) F RATIO(l) LSD(2> DUNMETT(3 > U M S ( 4) TESTIS .8841 .9093 8829 .8933 .103 .1153 .1408 .0072 THYMUS .2052 .2082 .2170 .2420 1.148 .0407 .0579 .0012 (1) Ratio of aaona- to uithin-srour variation-- one-factor analysis of varianca. ,2> sianificant difference-- aiven a sianificant <alrha0.05) F ratio anw too naans differina bo s o n than tha LSD ara sianificantlw different with a variable-wise falsa positive (alpha) arror rata of 0.05. (3) Dunnatt tast-- Ano traataant naan differina froa tha control aaan bo aora than tha Dunnett statistic is sianificantlo diffrantfroa the control aaan with a variable-olte falsa positive (alpha) arror rate of 0.05. (4) Within-sroup Mean Souara. + Sianificantlo diffrant (P<0.03> froa control drour bo LSD. Sianificantlo different (P<0.05) froa control sroup 3o Dunnatt ind LSD* tast * Sianificant at tha 0.05 probability laval. Company Sanitized. Does not contain TSCA CBt (FEMALE) - ORGAN I BODY WEIGHT MEAN ABSOLUTE DATA RATS SACRIFICES AFTER 10 EXPOSURES GROUP FINAL UGT. CONTROLS ' LOU <300 PPH) INT. (5.000 PPH) HIGH (30.000 PPH) F RATIO(l) LSD(2) DUNNETT(3) U N S (4) 222.0000 223.2000 210.4000 208.8000 2.432 14.3132 18.0054 117.1730 HEART .7780 .8640 .8260 .7360 1.772 .1071 .1354 .0066 LUNGS 1.3720 1.3780 1.4420 1.3700 1.678 .2181 .2706 .0265 LIVER 7.0640 8.3200 8.3640 8.3500 .731 a 1.1124 .4473 SPLEEN .6040 Z 1*0 .5 0 2 0 1.277 1163 .0075 GROUP KIDNEY THYHUS CONTROLS LOU (300 PPH) INT. (SiOOO PPH) HIGH (50F000 PPH; F RATIO(l) LSD<2> DUNHETT(3) UHSC4) 1.7880 1.8460 1.8320 1.7180 .801 .1782 .2210 .0177 .6020 .7160 .3740 .6740 1.220 .1767 .2173 .0174 (1) Ratio of aaon*- to within-aroua variation-- one-factor analval* of varianc*. Ia> L..t aianificant diff.r.nc a - a t v . n i.nificant anw two aaana differina b* ora than tha LSD ara aianifieantlw different uith a variable-wiae falaa aoaitive (alaha) arror rata of 0.03. (3) than Dunnatt taat-- Anw traataant aaan differina froa tha control an b oora tha Dunnatt atatlatic ia aianifieantlw diffrant froa tha control aaar. uith a variabla-wiaa falaa aoaitive (alaha) error rate of 0.05. (4>. Uithin-aroua Naan Sauara. , Sianifieantlw different (P<0.05> fro control aroua bw LSD. Sianifieantlw different (P<0.05) fron control arouP bw Dunnatt teat and LSD. Company Sanitized. Does no! contain TSCA CB (FEHALE) - ORGAN J BODY WEIGHT 1EAN RELATIVE "ATA RATS SACRIFICED AFTER 14-Da / OBSERVATION GROUP CONTROLS l0U <300 PPN) INT. <3.000 PPN> HIGH <30.000 PPH> F RATIOU) LSD(2) DUNNETT<3) WNS< 4) HEART .3346 .3341 .3704 .3333 .899 .0463 .0574 .0012 tMM?? .4989 .42434 .4370 .4394 2.173 .0404 .0749 .0020 LIVER 3.6533 3.7989 3.8730 3.8890 .407 5013 .4221 .1399 SPLEEN .2334 2323 .1939 .2332 1.722 .0469 .0382 .0012 GROUP CONTROLS LOU (300 PPN) INT. <3.000 PPN) HIGH <30.000 PPN) F RATIOU) LSD<2> DUNNETT(3) UHS<4> OVARIES .0602 0636 .0634 .0393 .167 .016L .0201 .0001 THYHUS .2647 3071 .2605 .2611 1.173 .0623 .0776 .0022 MONEY ..77951763 .S087 .7702 .638 .3841 .1044 .0039 *nw tL* M t t>Bifl*,n*0^ ^ * ^ n " * - - 4 t v # r r i i a n r n c M r ( r an* lv* 1* of ''`Anca. ?i:h u s s x s ? x f & ? TM ' x s i ; . r ' S . ' . T . s . - * - S H i u f t c a n t l * diffarant <P-:o.0 5 ) fro. i Sianiflcantl* diffarant (P'n n , # control aroun bi LSD. an* LSD. 4 ` 0 03, f|,o control araua Sa Dunnatt `a** -- Company Sanitized. Does not contain TSCA CBI  (FEMALE) - ORGAN 1 BODY UEZGHT MEAN RELATIVE DATA RATS 9ACRI:- ICED AFTER 10 EXPOSURES GROUP HEART LUNGS LIVER SPLEEN CONTROLS LOU <300 PPM) INT. <3.000 PPM) HIGH <30.000 PPM) F RATIO!1) LSD<2> DUMNETK3) U N S (4) .3314 .3877 .3914 .3626 1.466 .0480 .0596 .0013 .7C34 .7071 *863 .'>60 .423 .0869 .1078 .0042 4. "'831 3.8187 4 0637 4.2426 2.619 .3243 .4023 .0383 .2719 .2201 .2384 .2593 1.436 .0479 .0394 .0013 KIDNEY .3074 .3274 .3810 .9136* 3.908* .0768 .0933 .0033 GROUP THYMUS CONTROLS LON <300 PPM) INT. (3.000 PPM) HIGH (50.000 PPM) F RATIO!l> LSD<2> DUNNETT(3> u n s <4 ; 2698 .3212 .2727 .3233 1.230 .0792 .0982 .0033 (1) Ratio of on*- to uithin-aroua variation-- -one-factor analysis of variane*. <2> Laat sianificant differance--aiven a sisnificant (alrh#*0.05> F ratio, anw tuo a#an* differirti bu aora than th* LSD art aisnificantlu different uith a verieble-vi* fait* positive (alpha) irror rata of 0.03. (3) Ounnatt teat--Anu traataant aaan differina froa tn# control **an Du or* than th* Dunnatt tatiatic ia axanificantlu different fro th* control ear. with a varlable-wiea false positive (alpha) error rata of 0.03. (4) U i t h m - a r o u p Naan Sauer*. + Siflnificantlu different <P<0.05> froa control roup bu LSD. # SianifIcantlu diffrant <P<0.03> froa control arour bu Dunnatt teat and LSD. < Sisnificant at the 0.03 probabilit laval. Company Sanitized. Does no! contain TSCA CBt <FEMALE> - ORGAN ( BODY UEIGHT MEAN ABSOLUTE DATA RATS SACRIFICED AFTER 14-OAY OBSEK'J*-13N GROUP FINAL UGT. HEART LUNGS LIVER CONTROLS LOU <300 PPM) INT. <3*000 PPM) HIGH <30*000 PPM) F RATI0<1) LSD<2) DUNNETT<3) MHS <4) 201.6000 234.8000 263.4000 237.2000 .484 20.2670 23.1437 228.3000 .8740 .9040 .9840 .9100 .880 .1494 .1853 .0124 1.8260 1.59604 1.7420 1.7020 1.797 .2136 .2650 .0254 9.3800 9.6800 10.2700 10.0040 .363 1.3669 1.9439 1.3638 SPLEEN .6120 .3940 .3140 .6140 1.003 .1413 .1733 .0111 GROUP CONTROLS LOU <300 PPN) INT. <3*000 PPM) HIGH <30*000 PPM) F RATIOU) LSD<2) DUNNETT <3) UNS <4 > KIDNEY 1.9780 2.0180 2.1460 1.9760 .993 .2408 .2988 .0323 OVARIES .1360 .1620 .1680 .1320 .302 .0382 .0473 .0008 THYMUS .7000 .7820 .6880 .6720 .647 .1827 .2266 .0186 ?" tl? of ona" to " lthin-srour variation-- >ona-factor analysis of varianea. <2i Laast slanificsnt dlffaranca-- sivan a sisnificant <alrha0.05) F ratio* ant two aaanc dlffarin* bv ora than tha LSD ara sisnificantly diffarant uith a v t r u b l a - v m falsa aositiva <alrha> arror rata of 0.05. <3> Dunnatt last-- Anw traataant aaan diffarina froa tha control aaan bw aora than tha Dunnatt statistic is sisnifieantlw difft'snt froa tha control aaan m t h a variabla-uisa falsa aositiva (alaha) arror rata of 0.0!. (4) Ulthin-aroun Naan Sauara. + Sianificantls diffarant (P<0.03) froa control sroua bv LSD. i t s m f l c a n t l w diffarant <P-'0.05> froa control srour bv Dunnatt and LSD. tast Company Sanitized. Does not contain TSCA CBI