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AR 226 0877 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: A mixture containing perfiuorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-783. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot was not noted. The test sample is FC728.30%(cSou7l8t4o-n1e).foCaumrere,nt2.i3nf%orsmoadtiiuonmioncdtiyclastuelsfaittei,s a1.m6i%xtpuorleyoofxy1e.t0h%ylPeFneOS, mmoonnooobcuttyyllpehtehnear,l 0et.h0e5r,%0to.l1yt%risaozodlieu,manladur6y7l.su9l5f%atew,at2e5r..0% diethylene glycol The following summary applies to a mixture with incompletely characterized concentrationsof impurities. Data may not accurately reflect the toxicity of the fluorochemical componentof the test sample. METHOD Method: Not given Test type: Static acute GLP: No Year Completed: 1979 Analytical monitoring: Dissolved oxygen concentrations. Statistical methods: Results were determined by graphic interpolation. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution water: Deionized water. Synthetic sewage: Not given. Reference and test solution preparation: Not given. Test vessels: Notgiven. Number of concentrations: one plus 4 blank controls. Temperature: Not given. Total suspended solids and pH on day of testing: Not given. Element Basis: Respiration inhibition as determined by oxygen consumption. Remarks: Study terminated at 7 minutes,sincethere was no indication of toxicity at the highest sample concentration. --= RESULTS Nominal concentrations: Blank controls and 1000 mg/L 002607 Element values: No acute inhibitory effect on activated sludge respiration rate at 1000 mg/L with a contact timeof 7 minutes. Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported applytothat mixture and not the fluorochemical proportion alone. CONCLUSIONS The FC-783 exhibited no inhibitory effect at 1000 mg/L over a 7 minute exposure. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 3. Study lacks description and recordsofthe method followed. The inoculum is not properly characterized. Sample purity was not properly characterized and the study lacks analytical confirmation of the amountof fluorochemical proportion in the solution. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number 4896S, 1679. OTHER Last changed: 6/26/00 002608 . ie Lis J A L HLL i HE neet nEEEEE EE oe J a TN E E A eme t E ; Ne Eo HN[HH HE foal LS a LE Re . ee L l EE e R Bil Fo EEE Ie hf NN i ee os a TN RE Lame ee ETEHEIN Hp HEHE ooze09 [4- [A eerie. dE i IM. a Acute xreiry oF ACTIVATED, SLUDGE A campPIuND `v MIXED LIPUOR = wZ Lo 4536'S (6000)| 21L|-0e ) ooo) (0) | 32? |(62): " 3 WAL) D. o eQ 0 D, 10. Er iv ; 3 us 22 | 7.9 72.9 | 2.6 k;L I) 3.15 [1.357] i 1s | evs |nes `H2i 30 i) Ig i`s : cC.66 ||ee?ps 485| 5 3.7 [34 a25.6 |[a7g -4 0.5 i . i 3: : . : % BR . HS ae t seFag2 et PESe g [@= m apd 17 HANIA Ts Ta Sp 0) |=) 3 Go J $ \ 35, Q 3k<. 33 002610