Document 6RXoxr7pVq73KvpkRGZ6QYpnE

AR A A S _ C& 0 3 ACUTE TO XICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) TEST SUBSTANCE_______________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 502. The test sample is FC206CE (2/16/89). Current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. The following summary applies to a mixture with Incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the ftuorochemicaI component o f the test sample. METHOD:________________________________________________________ Method: OECD Method 202 and U.S. EPA Method EG-1 Type: Acute static GLP: No Y ear com pleted: 1989 Species: Daphnia magna Supplier: In-house cultures. Initial brood stock from Aquatic Bio Systems, Inc, Fort Collins, CO. A nalytical m onitoring: Temperature, pH, and DO Exposure period: 48-hours T est organism age: <24 hours old Statistical method: Not given. Test conditions: Dilution w ater: Carbon-filtered well water Dilution w ater chem istry: Tem perature: 21 C Dissolved oxygen: 9.0 mg/L pH: 7.9 Lighting: Not given. Stock and test solution preparation: Not noted. Exposure vessels: 250 mL Pyrex glass beakers containing 200 mL of test solution and a water depth of 6 cm. Num ber o f replicates: one Num ber o f daphnids per replicate: 10 Num ber o f concentrations: 4 plus two blank controls W ater chem istry during the study: pH range(0-48 hours): 7.9 - 8.3 (control exposure) 001730 7.9 - 8.0 (1000 mg/L exposure) Tem perature (0-48 hours): 20 C Dissolved oxygen range (0-48 hours): 9.0 - 9.2 mg/L (control exposure) 3.4 - 9.0 mg/L (1000 mg/L exposure) R E S U L T S ______________________________________________________ Nom inal concentrations: Two blank controls, 1, 1 0 ,1 0 0 , and 1000 mg/L Elem ent values: 48-hour E C 50= >1,000 mg/L Element values based on nominal concentrations Rem ards: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The FC-206CE 48-hour EC50 was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G 1358-2,1989. O T H E R __________________________________________________________ Last changed: 6/26/00 001731 BEST COPY AVAILABLE ENVIROiWENTAL LABORATORY DAPHNIA TOXICITY DATA SHEET. LR TYPF. /ftA fir CHB1ICAL TESTED^2^K ?^!g: ( /( m ) LOT^S ORGANISLZfertif/ffl. M /ard . AGE OF TEST ORGANIS4S:<24 h r s . o ld I n s t a r s (n e o n a te s). /fC ,___ DATE REC'D //Mit/ef. 9-IS PER TREATMENT /iO TEST VESSEL: 2SQ m l P v rex R p l a s s b e a r e r TEST SOLUTION VOLUME: 200 mLWATER DEPTH: .fi.o n TIME STARTED- DATE STARTED. V /V /fr? DATE COMPLETED V / o / y 9 DILUTION WATER C a r b o n - f i l t e r e d w e ll w a te r (Tem p.. / / .,, ? C , D.O. mg/L. p H _ 2 _ _ ) ANALYST -yMs/Zf E C ^ O S l C o n fid e n c e L im its ) -------------------------------- ------------- --- Test In itia l V essel Cone, No. mg/L X C ontrol n r CoMroi 11 z. h p io n 43 lo co T otal Amount Added *\ --- 0 .2 / Zj zn Zoo' 24 Hours 48 Hours T o ta l No. pH D.O. T o ta l No. Dead mg/L Dead D.O. mg/L O ft ft 0 0'0 __ f t 0 "n .o -' ' ft m r* 3$; <h( 5 r '>*> fiA 7 ? HA 7,7 HA 7, 7 'A,*/ - T e s t t T e n p l i ' & & 'z'n s i H f- Z#- r - Toxiclt rotocol 0 0 1 /4 ,