Document 6RKdmrYDQBRmakZxZxvgZ0Rm9

M AL6 - O Z ^ O ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FX-601. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was cc 835-34. The test sample is L-9235 (FX-601). Current information indicates it is a mixture of 1% PFOS, 12% diethylene glycol butyl ether, 81.25% water, 2% propane Sultone foamer, 1% sodium octyl sulfate, 1% sodium decyl sulfate, 0.1% N-(3- chloroallyl) hexaminium chloride, 0.8% xanthan gum, 0.8% starch, and 0.05% tolyltriazole. _ The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD_________ _______________________________________________ Method: Activated sludge protocol EAR/WAS 5-09-84 T est type: Aerobic G L P : No Y ear Com pleted: 1984 Analytical m onitoring: Dissolved oxygen concentrations S tatistical methods: Results were determined by calculation of the oxygen consumption rate. Test organism source: Activated sludge mixed liquor from the Metropolitan Wastewater Treatment Plant, St. Paul, MN Test condition: Dilution w ater: Deionized water. Synthetic sewage: Not given. Reference and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. T est vessels: Not given. Number o f concentrations: three plus blank control, in duplicate (two studies run) Total suspended solids and pH on day o f testing: Not given. Elem ent basis: Respiration inhibition as determined by oxygen consumption. RESULTS_______________________________ '_________________________ Nom inal concentrations: Blank control, 10 ,10 0, and 1000 mg/L. 002100 Elem ent values: No inhibitory effect on activated sludge respiration rate was seen at 1000 mg/L with a contact time of 7 minutes. Element values based on nominal concentrations. Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ ___ The material exhibited no inhibitory effects at 1000 mg/L over a 7 minute exposure. The respiration rate of the test bioreactor was higher than that of the blank controls. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking = 3. The test lacks record of methodology used. The method was not an agency-approved method. The inoculum is not properly characterized. There is a lack of characterization of the test sample and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_________________________________________ :__________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number B 1 6 8 4 ,1984. O T H E R __________________________________________________________ Last changed: 6/26/00 002101 3M Environmental Laboratory Acuta T o x ic ity o f a Compound (Substance) touA ctlvated Sludge Mixed Liq u o r Protocol Reference: EAR/WAS 5-09-84 Lab request no*: //> Date: /* A nalyst:' ^ * * T The a c tiv a te d sludge mixed J la u o r wps c o lle c te d from the S t. Paul/M Inneepolis Metro Wastewater P lant on . Sample d e s c rip tio n : ______ COMMENTS: * llUXXUUXAL. ID loo I0O fM C t^ S O O 3^003 30,03 300,IL. BEST COPY AVAILABLE 3H Environmental Laboratory Acuta T o x ic ity o f a Compound (Substance) to -A c tiv a te d Sludge Mixed liq u o r Protocol Reference* EAR/WAS 5-09-84, Lab request n o .: Date: ^ 2 0 / A n a ly s t*.-; The a c tiv a te d sludge mixed tlq u was/C lJected from the S t. Paul/MtnneapolIs Metro Wastewater P la n t on Sample d e s c r ip t io n : . . . ^ - P rep ara tion o f sto ck s o lu tio n : ^ grams o f sample were d ilu te d to w it h d e io n ise d reagent w a te r.e Dw wavte o fer p re p a ra tio n *.p n e | # w i w f c i w i i ^ ^ TTm t min. sec. T > i> s o t > .Q Q y s ftrx C CENTRATION OF TEST MATERIAl T n AC:Ti|l\VAT6D 1 lf li o /J l in o ^ g g j lt> r/^ J ? o n lf f5 l ml I \r~ a o t o 1t if i 1y / U - L o.Q U v . y 1 b \ ( r f 1 `U M LL g o \M \ \ 5 '% i t i <jS lLL\ k i2 = . LJ Q < - ? r it th l1 s. nAs.q 3 .7 \&n 2 2< -- dIL JL JM . 34Ln o \ . * > , 2 ) \ S > ,0 ?-<2~ o o U ,^ U V 3 n o //^ Z 'O / / ^ jS M - (7 /5 " 3 -' ! r?o t.n !. 0 .LM IQ on? I i S 1 O r f i 1 Ml // O S ' ML ML /*> ML LL M O 0 . I . I . B O . r * . JJM S i Olios COMMENTS* * \ u d io &jlq\ lo e ^ V v t s wjxJ -J WajxJ S O O yuJ IO 3 ,0 0 5 - lO O IO OO 30.05 3 0 0 ,3 BEST COPY AVAILABLE cU .... 0 0 2 1 3 . Form 10620-8-B PWO > Sample Description J^r<?Z&S (EXC*/) F /z ^ r^ /A T / a A J J ^ ^ o' K ~ Environmental Lab - Work Sheet ^/OX/?f/ J* ( V!ASC//O<1770A& s i 3s_ : Y -l& O Lab Requast Ne, Page _ _ _ / _ -- of___^ J Date:._ s / j z ^ s y Analyst: ^ - Q x v i y H roiA<^iw<^niA ( uta C ^ p l fu ir liu lw ) ^ v u J b s la u r/' __ _________________________________ C i =-CkvdtvnA `1 __________________ __________ f 'm d tr o A Z ___________________________________________ \/a l u i ^ V lL O o ^ tr ^ A m u ^ci l j |n n (m q u 0 .1 ^ 3 0 O tlp L fliz i |O O O v u a /^ ~~K T 2 ) _________________ _________________________O . ^ A ^ T _______________________________ l u M i^ h i+ tW mV - Z P > .0 ~ L *y */ & Io m i m T . 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