Document 6R9go5O3LgB2JRXZVVewZJMn4

ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE Identity:Mixturecontainingperfluorooetanesulfonawtheich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3003. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was notgiven.The testsample isFC-3003, identifiebdy the laboratorays R1331. Currentinformation indicatesitisa mixtureof1% PFOS, 15% diethyleneglycolbutylether, 79.39% water,2% Sultonefoamer, 1.5% sodium octylsulfate1,% sodium decylsulfate0,.06% sodium laurylsulfatea,nd 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the tluorochemkal component of the testsample. METHOD: Method: OECD Guideline203. Type: Acute static GLP: No Year completed: 1997 Species: Pimephalespromelas Supplier:In-houseculturemsaintainedatAScl-DETD, Duluth,MN Analyticalmonitoring: Temperature,pH, conductivitayn,d dissolved oxygen. Exposure period: 96-hours Statisticamlethods: The LC50 valueswere calculatedby Trimmed Spearman-Karber method. The NOEC valueswere calculatedby Fisher's Exact testusingToxstatstatisticsaolftware,Version 3.5. Test fishage: Juveniles Length and weight: Average length 16.6 mm Average weight 0.036g (wet) Loading: 0.12g/L Pretreatment:None Test conditions: Dilutionwater: DechlorinateCdityofDuluth,MN tapwater,post- carbon filterT.he waterwas aeratedfor24 hours priortothe test. Dilutionwater chemistry: Hardness: 48 mg/L as CaC03 Alkalinity: 44 mg/L as CaC03 Conductivity: 119 pmhos/cm pH: 8.08 Lighting:Cool-whitefluorescentlights.A dailyphotoperiodof 16 hours light and 8 hours darkwas maintainedthroughoutthetestingperiod. Stockand testsolutiopnreparationT:he testmateriawlas addeddirectly to thedilutiownater on a weight/volumebasis. Concentrations dosing rate:Once. Stabilityof the testchemical solutions: Not noted. Exposure vessels: 4 L glassjarscontaining3 L testsolutions. Number of replicates:2 Number offishper replicate:10. Number of concentrations: fiveplusa blankcontrol Water chemistry during the study: pH range (0-96hours): 7.68 - 8.08 (controelxposure) 7.60- 7.92(1000 mg/L exposure) Temperature range (0-96hours): 20.5- 20.7 'C Conductivityrange (0-96hours): 119 - 121 pmhos/cm (controelxposure) 133 - 136 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96hours): 6.9- 8.7 mg/L (controelxposure) 5.1- 8.6 mg/L (1000 mg/L exposure) RESULTS Nominal concentrations: Blank control,130,216, 360, 600, and 1000 mg/L Element values: 96-hourLC50 = 472 (376- 593) mg/L 96-hourNOEC = 216 mg/L Element values based on nominal concentrations Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hourLC50 forfatheadminnow was determined to be 472 mg/L witha 95% confidenceintervaolf376 to 593 mg/L. The 96-hour No Observed EffectConcentration(NOEC) is216 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking= 2. Testingmeets the criterifaorquality testing.However, sample puritywas notproperlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportioninthe solution. REFERENCES Thistestwas conducted by ASci Corporation,Duluth EnvironmentalTesting DivisionD,uluth,MN atthe requestofthe 3M Company, Lab Request number R1331,1997. OTHER Last changed: 6/26/00 ASclCorpomtion/AScl-Dulut EnvironmentTaelstinDgivision ASciStudy[D# 5010-M STUDY 3UL ACUTE TOXICITY OF R1331 TO FATHEAD MINNOW (Pimephalepsromelas) DATASTANDARD OECD GUIDELINE 203 STUDY COMPLETED June 26, 1997 TESTING FACILITY ASel Corporation/ASel-Duluth EnvironmentalTestingDivision 4444 AirparkBoulevard Duluth,MN 55811 Tel.No. (218)722-4040 Fax No. (218)722-2592 STUDY IDENTIFICATION NUMBERS AScl Study ID# 5010-068 3M Company Lab Request#R1331 Sponsor. 3M Company Sponsor Study ID# R1331 1.0GENERAL PUORMATION ASel Corpomtion/AScl-Dukith EnvironmentaTlestingDivision ASci Study[Df 5010-M StudyTitle DataStandard Sponsor Sponsor'sRepresentative Sponsor'sLab Request# TestingFacility PrincipalInvestigator ProjectDirector TestingFacilitDyirector DefinitivTeestDates Data Validationand ReportReview AcuteToxicitoyfR1331toFatheaMdinnow (Pimephalpersomelas). OECD Guideline203. 3M EnvironmentalTechnology and Safety Services,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133; Tel No. (612)778-7452. Susan Beach, 3M EnvironmentalTechnology and SafetyServices,Building2-3E-09,935 Bush Avenue, St.Paul,MN 55133; TelNo. (612)778-7452. R1331. ASCI Corporation/AScl-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040. Joe Dierkes Joe Amato Donald Mount June 17-21, 1997. Alan Mozol R eportSignature*. Retentionof Raw Data and FinalReport Locationof Raw Data and FinalReport Two yearsfrom thedatestudyiscompleted. AScl Corporation/AScl-DulutEhnvironmental TestingDivision,4444 AirparkBoulevard, Duluth,MN 55811;Tel.No. (218)722-4040. Sponsor: 3M Company SponsorStudy [D# R1331 2 ASclCorpomtiontASel-Duluth EnvironmentTaelstinDgivision A&I Study[D# 5010-M 2.0OBJECTIVE(S) TO determintehe96-hou1r0% and50% effecctoncentrati(oLnCsIOandLCSO),andthe96-hour no observableeffectconcentratio(nNOEC) ofthetestsubstanceR,1331, forfatheadminnow (Pimephalespromelas)under statitcestconditions. Ilis studywas conductedaccordingtotheAScl studyplanretainedby theSponsor and OECD Guideline203 (OECD 1993). 3.0TEST METHODS TestSubstance Test Organisms DilutionWater PropertiesR:1331, amber liquids,pecifigcravityca.1.0(water= 1), stablea,nd completelysolubleinwater. Testconcentrations0: (dilutiownater control),130,216, 360, 600, and, 1000 mgll. Test solutionpsreparedby mass additionof testsubstanceto dilutiownaterfollowedby vigorousstirring. Source: Healthy,juvenilefatbeadminnows (Pimephalespromelas)obtained from AScl-DETD in-houseculture. HoldinglacclimationT:he fishused inthetestswere obtainedfrom AScIs in-houseculture.The fishused in thestudywere hatchedbetween May 1'7 and 23, 1997. For the48 hoursprecedingtestinitiatimoonrtalitwyas 0%, and thefishwere not fed for24 hoursbeforetestinitiationT.he fishmean lengthwas 16.6 2.5 mm, and mean wet weightwas 36 19 mg. Loadingratewas calculatetdo be 120 mg of fishper L testsolution. Use intest:Used 10 organisms/repicateand 2 replicates/test concentration. Source: DechlorinateCdityof Duluth,MN, tapwater.The most recent chemicalanalysisisin Raw Data Package. CharacteristicsA:t testtime,thewater had a hardnessof 48 mgll (as CaCO3) and a pH of 8.08.Aeratedfor24 h priortouse inthetest. Sponsor. 3M Company Sponsor Study ID# R1331 3 Exposure Chambers Incubation Observations Endpoint Calculations ASciCorporation/AScl-Dulu EnvironmentTaelstinDgivision ASelStudyID# 5010-M 7ype:4-Lglasjsars. Use in test:3.0 L of dilutionwater or testsolution/chamber.During the test,keptcovered,except.whenexperimentalobservationswere made. Solutionswere notaeratedduringtheexposures. Duration: 96 hours. Dailyphotoperiod:16 h lightand 8 h dark, maintainedusingcoolwhite fluorescenltamps. Temperature: 21.0* I*C. Biological:Daily- Test organismswith abnormal behaviorand signsof stress,and dead testorganisms. Water chemistry:(1)At testinitiatiotno-talhardnessand alkalinity(,2) daily-temperatureD,O, conductivitya,nd pH. Alldeterminationwsere made accordingtoAPHA methods (1995). LCIO: InhibitioCnoncentratio(nlCp)Method. LC50's: Trimmed Spearman-KarberMethod. NOEC. Fisher'sExactTest (TOXSTAT statisticsaolftware,Version3.5, West Inc.,and Universitoyf Wyoming). Sponsor.,3M Company Sponsor Study [D# R[331 4 ASciCorpomtion/AScl-Dulut EnvironmentaTlestingDivWm ASclStudy[D#5010468 3.0RESULTS TestSubstanceToxicity(Pertinendtataarein Table 1) Ranges of Water ChemistryParameters 24-hLC.50: > 1000 mg/L (Not Calculable). 48-hLC50: > 1000 mg/L (Not Calculable). 72-hLC.50: > 1000 mg/L (Not Calculable). 96-hLCjo: 173 mg/L (NotCalculable). 96-hLC50: 472 mg/L (376-593). 96-hNOEC- 216 mg/L. Totalhardness (asCaCO3 mgll): 44-48. Alkalinit(yasCaCO3 mgll): 42-44. Temperature(*C): 20.5-20.7. DO (mglL): 5.1-8.7. pH: 7.60-8.08. c conductie!y(gmhE&ML 18-136. Sponsor: 3M Company SponsorStudyID# R1331 5 ASci Corpomtion/ASel-Duluth EnvironmentaTlestingDivision ASci StudyID# 5010-068 4.0TEST QA/QC QA/QC Criteria Data Mortalityincontrolexposuremust notbe more am 10%. The lowestDO concentratioanttest terminatiomnust be atleast60% of theair saturatiovnalueattesttemperature. 0% mortality. The lowestDO concentratiomneasured was 57% of theairsaturatiovnalueat21.0*C. The lowestvaluewas measured atthe72-hour testintervaflorthe 1000 mg/L concentration. At the96-hourintervatlheDO was 64% of saturationT.he reducedDO atthehightest concentratiodnidnot appeartoinvalidattehe testassignificalnetthaliatnyd acceptablDeO's occurredatthe600 mg/L testconcentration. Testrdurcationmust be 96 h. The testdurationwas 96 h. efRoeeplfstetidrnoeunnraecalet)S4iu.'bstanceToxicityTest(thistestis Controlchartforsodium chloridereference optional). I toxicittyestinigsinRaw Data Package. 6 Sponsor. 3M Company Sponsor Study M# R1331 ASciCorporation/AScI-Dulut EnvironmentTaelstinDgivision ASel StudyID# 5010-068 5.0REFERENCES American PublicHealthAssociatio(nAPHA). 1995.StandardMethods forthe xaminationof Water and Wastewater.APHA, Washington,D.C. OrganizationforEconomic Cooperationand Development (OECD). 1981/1993.OECD GuidelinesforTestingof Chemicals.OECD PublicatioInnformationCenter,Washington,D.C. Hamilton,M.A., R.C. Russo,and R.V. Thurston.1977.Trimmed Spearman-KarberMethod for EstimatingMedian LethalConcentrationisnToxicityBioassays.Environ.Sci.Technol.11(7): 714-719;Correction12 (4):417 (1978). USEPA. 1993.A LinearInterpolatiMoenthod forSublethaTloxicity:The Inhibition Concentratio(nICp)Approach. Version2.0. West Inc.,and UniversityofWyoming. ToxstatSoftwarePackage.Version3.5.Cheyenne, Wyoming. Sponsor: 3M Company Sponsor Study ID# R1331 7 ASelCorporation/ASel-Dulut EnvironmentTaclstinDSivision ASol StudyID# 5010-069 TABLE 1. ACUTE TOXICITY OF R1331 TO FATHEAD MINNOWS (P.promelas) ts@baitai@ oncentm idii 0 (control) 130 216 360 600 1000 CumulativeNumber of Dead Organisms.and Percent 24 b.: 48h.. h 0(0) 0(()) 0(0) 0(0) 0(0) 0(0) 0(0) 0(0) 1(5) 0(0) 0(0) 0(0) 0(0) 1 (5) 1(5) -3 (15) 3 (IS) 9(4S) Percentagesare in parenthesis. 06 h 0(0) 0(0) 4(20) 9(45) 9(45) 18(go) LETHAL AND NO-EFFECT CONCENTRATIONS WITH CORRESPONDING 95% CONFIDENCE INTERVALS tc's andNOEC 24-h LC30 48-h LC50 72-h LC50 96-H LCIO 96-h LC50 96-h NOEC Valtiesbased on nominal testconcentr,.itotns. Data(mg/L)l > 1000 > 1000 > 1000 173 472 216 95% confid@@ Not calculable, Not calculable Not calculable Not reliable 376-593 Not applicable Slionsor:3M Company SI)onsorStudy ID# R1331 8