Document 6R31yg742pEOqNxZG4GVKMz2R
ARRAG - 07/8
ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
IESTSUBS ~~ T~ ANC 0000E 000
Identity: ArefmeirxrteudretocaosntPaiFnOiSng, pFeCr-f9i5u,oroorocatsanaescuolmfponoanteen,twhoficFhC-m2a0y3.al(s1o-be Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: is FC-203.
The 3M production lot number was not noted. The test sample Current information indicates itis a mixture of 1.34% PFOS, 35%
dSiueltthoynleenfeoagmleyrc,ol3b%utysloedtihuemr,o3ct7y.l8s5u%lfawtaet,er0,.12%0%soedtihuymlelnaeurgyllycsoull,fa2t.e,66an%d
0.05% tolyltriazole.
The following summary applies to a mixture with incompletely crehfalreaccttteorxiizceitdycoofnctehentfrlautoirooncshoemfiicmaplurciotimepso.nDeanttaofmtahyentoetstacscaumrpaltee.ly
M0 ETHOD: 00000
Method: Standard Methods (APHA), 1970 Edition
Type: Acute static
GLP: No
Year completed: 1973
Species: Daphnia magna
Supplier: Analytical
In-house cultures maintained at monitoring: Temperature, pH,
Bionomics, Inc., conductivity and
Wareham, DO
MA..
Exposure period: 48-hours
Test organism age: 12: 12 hrs. old
Statistical method: TLeo (median linear regression equation.
tolerance
limit)
values
calculated
using
a
Test conditions:
Dilution water: Well water
Dilution water chemistry:
Alkalinity: pH:
35 mg/L as CaCOy 74
-
Lighting: Not given
Stock and test solution preparation: direct addition of test substance.
Exposure
concentrations
prepared
by
Exposure vessels: 250 mL Number of replicates: 4
beakers
with
200
mL
exposure
solution
Numberofdaphnids per replicate: 5
Numberof concentrations: five plus a blank control
FCeoroodp:hyFloiondw,actoenrs,iwstaisngaoddfaedhoatmorgaetneiozfa0t.i5onmUo/fLstaafrtteerritnrtoruotdfuoctoidonanodf test
substance.
Water chemistry during the study:
pH range (048 hours): 7.1
000546
DTiesmspoelrvaetduroexyrgaennger(a0n-g4e8 (h0o4ur8s)h:our2s)1:15.C3 -- 8.9 mg/L
RESOLYS
00
Nominal concentrations: Bk control, 560, 870, 1000, 1800, and 3200mg/L. Element values: 48-hour ECso = 1560 (1280 - 2140) mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
conclusions
The test substance 48-hour ECso was determined to be 1560 mg/L with a 95% confidence Interval of 1280 to 2140 mgiL.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
patAQuANTY
Reliability: Kiimisch ranking = 2. This study meets the criteria for quality stetsutdiyngl.acHkoswaenvaelyrt,ictahlecsoanfmiprlmeatpiuornitoyfwtahse anomtouprnotpeorflfyluchoarroacchteemriiczaeldparonpdortthieon
in the solution.
REFERENCES~~~
000000
Test was conducted by Bionomics, Inc.,of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1973.
o0 TWER 0000000
Last changed: 6/27/00.
000547
ai3 n \| TF :
Bea a o
oo
0
|
BIOASSAY REPORT
.
|
SUBMITTED TO
-
| MINNESOTA MINING & MANUFACTURING COMPANY
!
ST. PAUL, MINNESOTA
ii
! |
:
.
]
.
-
;
.
:
:
;
Ai CUTE TOXICITY OF .3M SANPLES"wa(Ae ANcDaeB) TO THE WATER FLiEA :
(Daphnia magna)AND SCUD (Gammarus fasciatus).
!
:
L
Jl
--
J
B79i0onoMmaiicns,StrIence.t
= WDaerceehmabme,r,Ma1s9s7a3chusetts
.
2
ii. ooosgl
. ~
.
)
.
;
3 J
i
METHODS AND MATERIALS
These investigations were performed at the aquatic
invertebrate toxicology laboratory of Bionomics, Inc.,
Wareham, Massachusetts. The susceptibility of the
water flea, Daphnia magna and scud Camarus fasciatus
to 3M samples designated as sample numbers A and B
(100% active) was evaluated under static conditions for
a 48 hour experimental period. Results were, expressed
as the median tolerance pu
nominal
concentration of the test conpoumd Inwatercausing note
50 percent mortality. The TLg, values and 95% confidence ny
intervals were calculated by converting the test
concentration and the corresponding observed percent
mortalities to logs and probits, respectively. These
values were then used to calculate a linear regression
.
equation.
&
Both Daphnia and Gammarus were obtained from laboratory
cultures and judged to be in excellent condition. Procedure_s
for the static bioassays adhered to the protocol described
by the 1970 edition of Standard Methods (APHA). Static
Daphnia and Gammarus bioassays were conducted in 250 ml
beakers with 200 ml of the experimental diluent. The
diluent water was obtained from a well source and was used
as such. The experimental chambers were maintained in a
.
|
3
000549
:
3
)
1
water bath at 21 1c. Five 12 12 hour old Daphnia
were introduced into each experimental beaker and five fourth instar Gammarids were introduced into each experimental chamber for the Daphnia and Gammarus bioassays,respectively.
In the Daphnia bioassays, food was added at a rate of
0.5 ml/liter after the introduction of the compound to
the test vessels.
:
.
Bioassays were conducted using four replicates of each
concentration for both compounds (A and B) for both species.
The pH.of the standard diluent was 7.1 and the methyl orange
alkalinity vas 35 ppm as CaCO. Dissolved oxygen values
for the various test vessels for both compounds ranged
from 8.9 initially to 5.3 mg/l at the end of the test.
:
.
w
x
Food was prepared by homogenizing 5 g of starter trout
food and 1 g of Cero-phyl in 100 ml of water.
J
:
.
Sosa
3
Hip
Co
!
y
RESULTS
The predicted 48 hour TLgy (i.e. that concentration of active compound that allows 50 percent survival to the experitiental animals) and 95% confidence intervals for Gammarus and Daphnia exposed to compounds A and B are presented in Table 1. A summary of the observed mortality of Daphnia and Gammarus exposed to various. concentrations of compound A is given in Table 2. The observed mortality of Daphnia ahd Gammarus exposed to experimental concentrations of compound B is given in Table 3,
:
3
&
J
Los 000558
SUBMITTED BY:
PREPARED BY:
APPROVED BY:
Bionomics, Inc.
|
790 Main Street
Wareham, Mass.
December, 1973 :
~s. Krogh Derr, Ph.D.
2 SwNeeOnS y Ndee see
Invertebrate Toxicologist &
Kenneth J. Macek, Ph.D.
Director
.
J
:
5:
Table 1 - Acute toxicity of 3 M compounds A and B to the water flea (Daphnia magna) and scud (Gammarus fasciatus) exposed for a 48 hour experimental period.
eeeeeee eer
eter ere
48 hour TLg,-mg/liter
= Sw pecies Comi pounAd nop m Comr pBow 5u55nd
DaphnwiaahmPal gna
31.1 (23.3:41.5) -
1560.0 (1280.0-2140.
t
GammSaorunsd fasciatus 15.4 (12,8-20.1) -
1'130.0 (843.0-1310.0
-- re-- re rr------ see-- r -- ------i-- eeeemer mmsot
)
Bioassay conducted at 21 1c, Daphnia, 12 12 hours old
at initiation of tests.
:
PBioassays Conducted at 21C 1, Gammarus, fourth ingtar at
initiation of tests.
95% confidence intervals.
|
000553
Sg
A.
y
=
d)
i
.
Table 2 - Concentrations tested and corresponding observed
percent `mortalities for the water flea, (Daphnia magna), and such (Gammarus fasciatus) exposed to
3M compound A for a 48 hour experimental period.
Species
_----m Water Flea
(Daphnia magna)
Concentration
42.0
32.0
24.0
12.0 10.0
7.5
Control
Scud
' 32.0
(Gammarus fasciatus) 24.0
18.0
12.0
7.5 |
Control
-_--
%HariSiLly Shsbevet
.
100
60
0
*
0
0
0
:
0
;
100
:
70
. 60
50
=
0
0
J
:
.
5
.
Table 3 - Concentrations tested and corresponding observed
Y
percent mortalities for the water flea (Daphnia magna)
and scud' (Gammarus fasciatus) exposed to 3M compound
B for a.48 hour experimental period.
species
Concentration
% rorcaliey gbaerved
"Vater fla
3200.0
100
(Daphnia magna)
1800.0
-
4s
oe
1060.0
5
870.0
0
.
560.0
0
Control
0
3
Scud
3200.0
.
(Gammarus fasciatus) 1800.0
1000.0
870 0
+
560.0
Control
2
100
9,
4st
.
40
0
)
0
-
reece
reseeeeesereeeenemies
)
-
000555