Document 6QqmbB4R5Y2XY8jZ6Epa2vdd

TRADE SECRET AR226-3196 DuPont-8862 Study Title H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna Laboratory Project ED: DuPont-8862 A u t h o r : Laurie D. Bouchelle S t u d y C o m p l e t e d o n : November 30,2001 P e r f o r m i n g L a b o r a t o r y : E.I. du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 Company Sanitized. Does not contain TSCA Cjf H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna DuPont-8862 CERTIFICATION We, the undersigned, declare that this report provides an accurate evaluation of data obtained from this study. W ork Completed by: Laurie D. Bouchelle Associate Scientist Issued by Study Director: OL J~J Alan Samel, M.S. Research Ecotoxicologist Date 3o A > Zoo! Date Company Sanitized. Does not contain fSCA CSi -2- H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna STUDY INFORMATION Submitter's Notebook Number(s):| Haskell Number: 25134 CAS Registry Number: DuPont-8862 Physical Characteristics: Dark brown solid Sponsor: E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. Tn-T,ife Tnitiated/Completed: November 27, 2001 / November 29, 2001 Company Sanitized. Does not contain i SCA CBf -3 - H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna DuPont-8862 SUMMARY The acute toxicity of H-25134 to the water flea, Daphnia magna (less than 24 hours old) was determined in an unaerated, 48-hour, static test. The study was conducted with 4 concentrations of H-25134 and a dilution water control at a mean temperature of 20.3C (range of 20.2-20.6C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control was clear and colorless at test start. The 1 and 10 mg/L test concentrations were clear and colorless with undissolved test material on the water surface at test start. The 100 and 1000 mg/L test concentrations were slightly cloudy with undissolved test material on the water surface and on the bottom of the test chambers at test start. All water quality parameters were within acceptable limits during the exposure. Exposure of H-25134 to the water control and nominal H-25134 concentrations of 1, 10, 100, and 1000 mg/L resulted in 0, 0, 0, 10, and 10% immobility, respectively, at the end of 48 hours. No immobility or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no immobility at test end was 10 mg/L. The results are summarized as follows: Nominal concentrations of H-25134, mg/L dilution water control, 1, 10, 100, and 1000 48-hour EC50 for H-25134, based on >1000 nominal concentrations, mg/L H-25134 exhibited low concern for aquatic hazard3in an unaerated, 48-hour, static, acute test using the water flea, Daphnia magna (less than 24 hours old). Smrchek, J., Clements, R., Morcock, R., and Rabert, W. (1993). Assessing ecological hazard under TSCA: methods and evaluation o f data. Environmental Toxicology and Risk Assessment, ASTM STP 1179. (W.G. Landis, J.S. Hughes, and M.A. Lewis, Eds.), pp 22-39. American Society for Testing and Materials, Philadelphia. Company Sanitized. Does not contain TSCA -4 - H-25134: Static, Acute, 48-Hour Screening Test to Daphnia magna DuPont-8862 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal H-25134 Concentration (mg/L) Test Initiation (0 hours) Dissolved Oxygen (mg/L) pH Test Completion (48 hours) Dissolved Oxygen (mg/L) PH_____ Water Control 8.6 7.9 8.7 7.9 1.0 8.2 7.7 8.6 7.8 10 8.1 7.7 8.6 7.8 100 8.1 7.6 8.6 7.8 1000 7.9 7.6 8.5 7.8 TABLE 2 IMMOBILITY OF Daphnia magna, IN AN UNAERATED, STATIC, ACUTE, 48-HOUR TEST WITH H-25134 Nominal H-25134 Concentration (mg/L) Water Control 1.0 10 100 1000 Number of Dead Test Organisms / Number of Test Organisms at Study Start 24 Hours 48 Hours 0/10 0/10 0/10 0/10 0/10 0/10 0/10 1/10 0/10 1/10 Company Sanitized. Does not contain TSCA CBI