Document 6JKa5MJ26JNzrLZZ9jq6794

A R W -o H IJ ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: [2-(N-Ethylperfluorooctanesulfonamido) ethyl acrylate; may also be referred to as B1228, D-1, EtFOSEA, or FX-13. (2-Propenoic acid, 2[ethyl[heptadecafluorooctyl)sulfonyl]amino]ethyl ester, CAS # 423-82- 5) Remarks: Material is an amber solid. Sample purity and not recorded. 3M lot number 13. METHOD Method: Not given Type: Static acute range-finder GLP: No Year completed: 1984 Species: Pimphales promelas Supplier: Brady, Neb. Analytical monitoring: Nominal concentrations only. Temperature, pH and dissolved oxygen recorded. Exposure period: 96-hours Statistical methods: LCso could not be calculated as there was insufficient toxicity at concentrations above the water solubility. Test fish age: Not given Length and weight:: Length: 3.79 cm Weight: 0.359 g Loading: 0.6 g/L Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry during the study: DO: 6.5 - 9.3 mg/L pH: 7.9-8.2 Test temp, range: 21-22C Stock and test solution preparation: Test solutions prepared by mass addition of test substance to 3L of dilution water. Concentrations dosing rate: Once Stability of the test chemical solutions: Not recorded Exposure vessels: Wide mouth one gallon glass jars Exposure volume: 3 liters Number of replicates: one Number o ffis h per replicate: five 000537 Number of concentrations: Four plus a negative control RESULTS Nominal loading concentrations: Bk control, 10, 100, 500, 1000 mg/L Element value: 96-hour LC50 > 1000 mg/L Percent Moritality Nominal Test Concentration, mg/L 24-Hours Negative Control 10 100 500 1000 0 0 0 0 0 48-Hours 0 0 0 0 20 72-Hours 0 0 0 0 20 96-Hours 0 0 0 0 20 Remarks: All values reported are for nominal concentrations. No measurement of test concentrations was conducted. CONCLUSIONS The Et FOSEA 96-hour LC50for fathead minnow was determined to be > 1000 mg/L DATA QUALITY Reliability: Klimisch ranking 2. This study, while conducted using the methodology acceptable at the time, lacks analytical data for determination of the test substance concentration in the test solutions and determination of the sample purity. Testing was also done well above the solubility limit of the test substance. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number B1228, 4/1/84 OTHER Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 Last changed: 5/22/00 000538 ENVIRONMENTAL LABORATORY CHEMICAL ENVIRONMENTAL EFFECTS EVALUATION TOXICITY TEST: SUMMARY REPORT C hem ical T ested p y KOT IS T e s t Type= A yMXH' {p . t e i a t o ^ C ^ y E C s o /I C s o /G R s o (95% C . L . I : ^ ; / / l / L A / P r t m t \M /W /S )/Y 1 m q /1 T e s t S p e c i e s ! f r m je S M MtMAJOu) t r , a - i r m f i / ) T e st Pate-.- ^ - J K - <-/ / J - J - f v J L .R . 7 ^ /J -? g W A n a ly st(s ) : ( M 'A J ____________ su r e/T e st C on d ition s) Exposure Type: S ta tic_ \ / S ta tic/C o n tin u o u s ________________ O r b i t a l S h a k in g _________ S t a t i c R e c i r c u l a t o r y _____ S t a t i c R en ew a l__________ F lo w -T h r o u g h D ilu en t: * Si A erated W ell W ater ( I n i t i a l P .0 . % iw g /1 ; p H <P{,2 a Tem p. "Z / C ( /7?Q F ) ) W ater H ard n ess: ppm H a r d n e ss C l a s s i f i c a t i o n : ___________ D isso lv e d Oxygen % S a tu r a tio n : 4 8 -h r 9 6 -h r E xten d ed E xp osu re C on trol( s) Low est E xperim ental Dose IQ k ^ d H ig h e s t E x p e r im e n t a l D o se 1 0 0 0 inqUi pH L e v e l s : J f. 4 8 -h r J& S- 9 6 -h r E xten d ed E xp osu re C on trol(s) Lowest E xperim ental Dose H ig h est E xp erim en tal Dose lO O O ^ tf T e s t T e m p e r a tu r e C ( F ) : _ 8 iL jS jI Q xZ j 0 S t-L P h otop eriod : 4 8 -h r 9 6 -h r E x ten d ed E xp osu re ^-lcno>) g c n z i #C( ) S ix te e n hours lig h t w ith a 15-30 m inute tr a n s itio n p e r io d . BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET EXPLORATORY (RANGE-FINDING TEST) ly pe T e st O rganlsm ^ a t g M/MOo)touYYTYf& yteT. Pe t e R ec'd A vg, Wt. grains A vg. S i z e ^ f f i C M N o. O rg a n ism s p e r d o s e _____5_ T e s t S o l u t i o n V o l u m e _ 3 j , i t e r s / A l l - G l a s s Tank Tank Dimensions:WIDE-MOUTH 1 GALLON GLASS JAR Time S t a r t e d Date S ta r te d D ilu tio n W ater C arb on -fi l l e d w e llw a t e r (Temp. 2/ D ate Com pleted C ,. D.O. ppm, pH ft.2\ (95% C o n fid en ce L im its ) yiQ Q O m C f/f __ t x t :-- lk Cone. - */* T otal Amount Added 24 Hour s T otal D .O . N o . Dead pH ppm 48 Hours --------- 7 0 5 3 ? S T otal D.O. T o ta l I ) . 0 , N o .D ea d pH ppm N o .D ea d d H ppm ___ i 9 6 Hou :s T otal D.O. N o.D ead >H ppm C ontrol ... - - J L _____ J i i L . < m o ,j 2 Q Q _ . - 7 2 I . S O O - .... }OOQ .&CQLL. -- til . a f i l l Y/P n ft\ p . c fa$ ... n fnO b,(n o r? 7C s . (ftfy Bi h i a. f/2K o % UU- n f.x m n ic & *r (rl ?r /nrf ] w? , \ / 8i / Ri' / ?n 6rV T e s t Temp. C . . . - 2 J C L ... In L tla U 8 Date Comments:^ . j ^ 0 C W ) L J U f e l f y n ^ -_ 2 fP ~ L iz z Z L , .'SS3/2l/ L ^ T ^ L lK lU E ffe c ts o th er than M ortality EC5 0 - Tank No. Symptoms o r R e a c tio n s LC o n tr o l 1 2/ ;*s 24-H r. y/ vU^ ..... --------* / T otal No. A ffected ,, 48 Hr. 7 72-H r. , 96-H r. T7 ^__________ O v V u. / AKr - - A 1/5 A f f o\ c ^ . ________ M oo/ _________ _____________ V Lommonts