Document 6BbpNKmdo1N1nodQmrLdR8yQo

M AS.6 - O ACUTE TO XIC ITY TO FISH TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate;, which may also be referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 502. The test sample is FC206CE (2/16/89). Current information indicates it is a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctylphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample. METHOD:________________________________________________________ Method: OECD Method 203 and U.S. EPA Method EG-9 Type: Acute static-renewal GLP: No Y ear com pleted: 1989 Species: Pimephales promelas Supplier: Aquatic Bio Systems, Inc., Fort Collins, CO A nalytical m onitoring: Temperature, pH, and DO Exposure period: 96-hours S tatistical m ethods: Not given. T est fish age: Juveniles Length and weight: Average length = 19.9 mm Average weight = 0.06 g (wet) Loading: 0.20g fish /L Pretreatm ent: None T est conditions: Dilution w ater: Carbon-filtered well water Dilution w ater chem istry: Tem perature: 20 C DO: 9.6 mg/L pH: 8.0 Lighting: Not given. Stock and test solution preparation: The test material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Daily S tability o f the test chem ical solutions: Not noted Exposure vessels: 4 L Pyrex glass cylindrical tanks containing 3 L of solution. Num ber of replicates: 2 001736 Num ber o f fish per replicate: 10 Num ber o f concentrations: one plus a blank control W ater chem istry during the study: pH range (0-96 hours): 7.9 - 8.0 (control exposure) 7.9 - 8.0 (1000 mg/L exposure) Tem perature range (0-96 hours): 20-21 C Dissolved oxygen range (0-96 hours): 7.8 - 9.6 mg/L (control exposure) 3.6 - 9.6 mg/L (1000 mg/L exposure) RESULTS______________________________________ Nom inal concentrations: Bk control and 1000 mg/L Elem ent values: 96-hour LC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ___________________________________________________ The FC-206CE 96-hour LC50 for fathead minnow was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G1358-2,1989. O T H E R ___________________________________________________________ Last changed: 6/26/00 001737 JCiNVIKUINMfcl'AL LAUKIUKY FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) I.R , TYPE T E S T TRSTFD ____f/jb/ft9 trrr SfiZ. TEST ORGANISM H t f a r f tiflMGti___R O i ^ O A T F . RFJVn *//< $ /> .BATCH NO.^dSZ. AVG. W.QiQtoCl-aQlSsrm^ AVG. SIZE M SS^A JitJcSL?' NO. OF OGRANISMS PER TREATMENT /O LOADING FACTOR___C 'J rQ - _ g /L , FISH DISEASE TREATMENT A T M E ___________________ TEST VESSEL: Liter, frrex * glass, qdinririral.tank TEST SOLUTION VOLUM E_JS_JL TIME STARTED 2 u 3 t o t C - .. DATE STARTED < $ //i f t 9 DATE OTMPI.FTEn ^ /a<T/7R DILUTIONWATER C a r b o n - filte r e d .w ell w ater C T e n y .,Z Q .-JC , D.Q. mg/ L r pH Z Q ) A N A L Y L C s o( 95% Confidence T.-imitO Tank I n i t i a l T o t a l _______ 24 Hours___ 48 Hours 72 Hours 96 Hours No. Cone. Amount T o ta l Nc. pH D.O. T o ta l No. pH D.O. r o t a i N. PH D.O. T o ta l No. pH D.O. mg/L AMTd3daed Dead mg/L Dead mg/L Dead mg/L Dead mg/L X Control n I ff,if, O - - w %t O S.O O 1 ft,A, JL_ -- * n I P>.2j Q . I nW , l \Artn ?*nnn n I \n Ci I <%( n Ci . 1 O I t, lrmn *OO I O 1 ,v< O 2/? cit( O Iti T e st Temp. C I n it ia ls Date Z ~ ^ 3 s/zz/m Z Z-! ^ s .5//3m - K * ZjO T5 COMENTS: . L^lInnyO rtH ^ iuLsrlhl^ utUu LOafpr. IrtitiofspU o f IQflOwifl/LTX^---------------------------------- SECONDARY EFFECTS (OTHER THAN MORTALITY) lank No. X P 1 Symptoms o r R eactio n s 24-Hr. / A T o ta l Numoist A ffe c te d - " 48-Hr. 72-Hr. / 96-Hr. *< 3 r-- w cy / .. T e st m ethodology conforms with th e fo llo w in g two t e s t p ro to c o ls: (1) OECD #203 " F i s h , Acute T o x ic it y T e s t " . (2) US-BPA #BG-9 "Fish Acute Toxicity Test". BEST COPY AVAILABLE 001738