Document 6BX3Qm9KoJweojgLkdo1gEvZ1
p. 1 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
Quantitative Peak Review
1^ 7
K1000911 -013
5.30
PFBA 1 213.0/169.0 Da Baseline Missed i : Wronn Peak
.. ( 4.25
1i
13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak ,..i 6.31
...... L ... ............
PFBS 1 299.0/80.0 Da Baseline Missed i : Wrnnn Peak
l
180-PFHS 403.0/103.0 Da Baseline c Missed n Wronn Peak i 5.^3
;J ri
3.60 ...Jl . .
PFPeA 1 263.0/219.0 Da Baseline u Missed n Wronn Peak
ia
i JS m *
PFHxA 1 313.0/269.0 Da c Baseline c Missed i- Wronn Peak
i 6| 1
ai
13C-PFHxA 315.0/270.0 Da u Baseline Missed
Wronn Peak 5.30
> '= ;
[
13C-PFHXA 315.0/270.0 Da
Baseline c Missed r Wronn Peak
,j 6,^1
PFHpA 1 363.0/169.0 Da
l: Baseline Missed r Wronn Peak 6 .3^1
p; i
13C-PFHxA 315.0/270.0 Da
Baseline Missed n Wronn Peak
; 7.64
PFHxS 1 399.0/80.0 Da
u Baseline u Missed p Wronn Peak
j 7.64
180-PFhis 403.0/103.0 Da Baseline u Missed p Wronn Peak , J 7.64
1 l
jd l
S 180-PFHS
13C-PFDA
S 13C-PFDA
403.0/103.0 Da
515.0/470.0 Da
515.0/470.0 Da
Baseline c Missed
c Baseline Missed
Baseline Missed
_____ i Wronn Peak_____ _____ n Wronn Peak_____ _____ n Wronn Peak_____
Before After
13C-PFDA 515.0/470.0 Da g Baseline Missed p Wronn Peak
Page 99 of 245 137
P-2 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
--4.27....
K1000911-013
3.60
PFBA2 213.0/169.0 Da Baseline c Missed u Wrong Peak
PFPeA2 263.0/219.0 Da Baseline Missed c Wrong Peak
PFBS 2 299.0/99.0 Da Baseline Missed Wrong Peak
: "l
j|1 A ^
... j
I
j
J /V
j J -.-
!
Vi
.... 0 .Cf.. -(!
iH
' 1........
PFHpA 2 363.0/319.0 Da Baseline Missed l Wrong Peak
PFHxS 2 399.0/99.0 Da Baseline Missed u Wrong Peak
S 13C-PFDA 515.0/470.0 Da g Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
fg-l'V'VWVyv* ... .1^* ~ . ...-- PFHxA2 313.0/119.0 Da Baseline g Missed Wrong Peak
ihii imagesnotsalable
N/A Baseline Missed
Wrong Peak
Page 100 of 245 138
P-3 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrum ent Name
Ewan's Projects\PFCs 02081 OtASamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name A c q u is itio n Date A cquisition Method Injection Vial
K1000911-014 2/8/2010 10:32:18 PM PFCkinetexLong.dam 54
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown
1 .0 0 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 2263700 1935200 5611700 2227400
92742 5611700 114340 114340 114340
Analyte RT (Exp RT ) 0.00(1.36) 0.00(2.42) 2.38 (2.53) 0.00 (3.34) 4.13 (4.06) 0.00(4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74)
Analyte Area
0 0
13929
0
5395
0 0 0 0
Calc. Cone (ng/mL) N/A N/A 0.038 N/A 0.040 N/A N/A N/A N/A
Modified? Mo Mo Mo No No No No No Mo
e <y
,.
Page 102 of 242 139
Columbia Analytical Services1
p.4 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
IRC Summary
i Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 SJ3C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 0.00 (1.36) 1.36 (1.36) 2.47(2.44) 2.30 (2.44) 2.30 (2.53) 2.32 (2.53) 0.00 (3.34) 3.30 (3.34) 0.00 (4.06) 4.10(4.06) 4.29 (4.10) 4.79 (4.64) 4.74 (4.64) 5.33 (5.13) 5.74 (5.56)
Area
0
2263700 170
1935200 3022
5611700
0
2227400
0
92742 273 870
114340 262 182
Modified?
Calc (Exp) MRM Ratio
No 0.00 (2.38)
Ratio Confirms ID
No
No 0.00(50.17)
No
No 0.00(0.28)
No
No 0.00(19.17)
No
No 0.22 (0.32)
No
No 402.88 (22.53)
No
No 0.00(0.15)
No
No 0 .0 0 (2 2 .1 0 )
No
No 0 .0 0 (0 .2 2 )
No
No 17.19(1.86)
No
No 0.00(0.32)
No
No 0.00(0.29)
No
No 0.00(3.08)
No
No 0.00(0.32)
No
No 0.00 (0.36)
No
Page 103 of 242 140
Columbia
A n a lytica l S o rm e s 1
Quantitative Peak Review
Created with A n a lyit Reporter Printed: 14/02/2010 6:41:00 PM
K1000911-014
Before After
141
Page 104 of 242
jT i C o lu m b ia
4L-J rslytcai Sorvicos"
Ion Ratio Confirmation
Created with Anafyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000911-014
PFOA2 413.0/369.0 Da d Baseline c Missed c Wrong Peak
i', ~v}\ ,T Tu
PFNA2 463.0/219.0 Da o Baseline o Missed Wrong Peak
PFOS2 499.0/99.0 Da d Baseline Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline u Missed Wrong Peak
PFUnA2 563.0/269.0 Da Baseline Missed u Wrong Peak
4.29
PFDS 2 599.0/99.0 Da c Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da Baseline Missed u Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline o Missed l: Wrong Peak
Before After
Page 105 of 242 142
Columbia Analytical Services1
Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
P ro je c t D ata File R esult Table Instrum ent Nam e
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
S am ple Nam e A cquisition Date A cquisition M ethod Injection Vial
K1000911-014 2/9/2010 8:50:50 AM PFCkinetexShort.dam 54
Injection Volum e Sam ple Type D ilution Factor W eight to Volum e
5 Unknown
1 .0 0 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
130-PFHxA
PFBS 1
180-PFHS
PFHxA 1
130-PFHxA
PFHpA 1
130-PFHxA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
SJ3C-PFDA
13C-PFDA
IS Area 2638300 3308200 2977200 3308200 3308200 2977200 4233000 4233000
Analyte RT (Exp RT ) 1.26 (1.27) 3.60 (3.60) 4.26 (4.26) 5.29 (5.31) 6.23 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 28036 24414 4726 53347 15031 39048
2977200 4233000
Calc. Cone (ng/mL) 0.04 0.06
0 .0 2
0 .1 0
0.06 n ki0.03 0.80
1.00
Modified? No No No No NIO No No Mo
/T
'P' ( ( ^
Page 101 o f 245 143
p. 8 Created with Analyst Reporter
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IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp R T ) 1.26(1.27) 3.60 (3.60) 0.00 (4.26) 5.34(5.31) 6 .2 1 (6 .2 2 ) 6.31 (6.32) 1.26(1.26) 5.30 (5.30)
Area 28036 24414
0
5516 238640 16817 2638300 3308200
Modified?
Calc (Exp) MRM Ratio
No 1 .0 0 (1 .0 0 )
Ratio Confirms ID
YES
No 1 .0 0 (1 .0 0 )
YES
No 0.00(0.40)
No / uI l
No 0 .1 0 (0 .1 0 )
YES
No 15.88(3.03)
NO ii/j
No 0.43(0.40)
YES
No 94.10(13.55)
No
No 62.01 (13.06)
No
Page 102 of 245 144
Columbia Analytical Services
p. 9 Created with Analyst Reporter Printed: 15/02/2010 2 34:00 PM
Quantitative Peak Review
, ; 1.26
...., . L , ..... . ^
1.26
i
PFBA 1 213.0/169.0 Da l Baseline l Missed n Wronn Peak
" ;|
13C-PFBA 217.0/172.0 Da d Baseline Missed n Wrnnn Peak
; Z. j
4.26
' , . . . - jL _ ...
1
1
PFBS 1 299.0/80.0 Da u Baseline u Missed r Wrnnn Peak
180-PFHS 403.0/103.0 Da g Baseline g Missed n Wrnnn Peak j 5.^3
PFHpA 1
363.0/169.0 Da i_ Baseline Missed
i Wronn Peak 6.^1
13C-PFHxA 315.0/270.0 Da U Baseline c Missed n Wrnnn Peak
7.64
K1000911-014
j .II
3.60 ,.i .....,,....A--
PFPeA 1 263.0/219.0 Da a Baseline l Missed n Wronn Peak
d 5f
4 ! ........... i
...........
13C-PFHxA 315.0/270.0 Da o Baseline a Missed n Wrnnn Peak j 5.30
:j |
PFHxA 1 313.0/269.0 Da u Baseline Missed n Wrnnn Peak
13C-PFHxA 315.0/270.0 Da o Baseline Missed n Wrnnn Peak
1I
PFHxS 1 399.0/80.0 Da g Baseline u Missed ri Wrnnn Peak :,l 7.^4
>l |
18 0 -P F H S 403.0/103.0 Da c Baseline u Missed r Wrnnn Peak
7.^4
I
S 180-PFHS
13C-PFDA
S 13C-PFDA
403.0/103.0 Da
515.0/470.0 Da
515.0/470.0 Da
u Baseline g Missed
c Baseline Missed
c Baseline Missed
_____ r Wrnnn Peak_____ _____ n Wrnnn Peak_____ _____ r Wrnnn Peak_____
Before After
13C-PFDA 515.0/470.0 Da Baseline Missed i ' Wrnnn Peak
Page 103 of 245 145
Created with Analyst ^Reporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
K1000911-014
T .
"1 .-j 3.60
;
9 1 -0.j
.>
ft J^
....
PFBA 2 213.0/169.0 Da l Baseline Missed g Wrong Peak
..i 6-|1
PFPeA2 263.0/219.0 Da Baseline o Missed Wrong Peak
PFBS2 299.0/99.0 Da c Baseline Missed Wrong Peak
-I
PFHxA2 313.0/119.0 Da Baseline Missed c Wrong Peak
; ;l- L J
6| 1 9 T "
PFHpA 2 I P F H x S 2
363.0/319.0 Da
399.0/99.0 Da
i_ Baseline c Missed
Baseline Missed
o Wrong Peak I Wrong Peak
S 13C-PFDA 515.0/470.0 Da Baseline Missed g Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
Tliisstragesnot.ivalable
N/A Baseline Missed
Wrong Peak
Page 104 of 245 146
p. 11 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrum ent Name
Ewan's ProjectsVPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name A cquisition Date Acquisition Method Injection Vial
K1000911-015 2/8/2010 10:39:52 PM PFCkinetexLong.dam 55
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown
1.00 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFD0A
PFTrA 1
130-PFDoA
PFTeA 1
13C-PFDOA
IS Area 3008200 2230200 5767700 2614100
90636 5767700
121000 121000 121000
Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.30 (2.53) 0.00 (3.34) 4.12 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74)
Analyte Area
0 0
34416
0
4850
0 0 0 0
Calc. Cone (ng/mL) N/A N/A 0.071 N/A 0.035 N/A N/A N/A N/A
Modified? No No No Mo Mo No Mo No Mo
do *
Page 106 of 242 147
f i C o lilftlb la A n a ly tic a l S ^ n /io s r
p. 12 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
IRC Summary
. Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 1.30(1.36) 1.35(1.36) 2.36 (2.44) 2.26 (2.44) 0.00(2.53) 2.31 (2.53) 0.00(3.34) 3.28(3.34) 0.00 (4.06) 4.09 (4.06) 3.94 (4.10) 4.73 (4.64) 4 .7 4 (4.64) 5.21 (5.13) 5.72(5.56)
Area 9216 3008200 510 2230200
0
5767700
0
2614100
0
90636 285 1744
121000
141 893
Modified? No
Calc (Exp) MRM Ratio
0.00 (2.38)
Ratio Confirms ID
No
No 0.00(50.17)
No
No 0.00(0.28)
No
No 0.00(19.17)
No
No 0.00(0.32)
No .
No 167.59 (22.53)
No
No 0.00 (0.15)
No
No 0 .0 0 (2 2 .1 0 )
No
No 0 .0 0 (0 .2 2 )
No
No 18.69(1.86)
No
No 0.00 (0.32)
No
No 0.00(0.29)
No
No 0.00(3.08)
No
No 0.00(0.32)
No
No 0.00(0.36)
No
Page 107 of 242 148
Quantitative Peak Review
PFOA 1 413.0/169.0 Da Baseline l Missed r Wrnnn Pnak
13C-PFOA 417.0/372.0 Da
g Baseline o Missed ___n Wrnnn Peak___
2.31
PFOS 1 499.0/80.0 Da Baseline c Missed n Wrnnn Peak
I1
13C-PFOS 503.0/80.0 Da Baseline c Missed n Wrnnn Peak
p. 13 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000911-015
12.26
PFNA 1 463.0/419.0 Da Baseline u Missed n Wrnnn Peak
f* '!. ,'V ',!V>'Va
13C-PFNA 468.0/423.0 Da a Baseline Missed ___n Wrnnn Peak
3.28
PFDA 1 513.0/469.0 Da Baseline Missed
n Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak
PFUnA 1 563.0/519.0 Da c Baseline c Missed n Wrnnn Peak
v
PFDoA 1 613.0/569.0 Da c Baseline o Missed i~ Wrnnn Peak
..L,L 13C-PFUnA 565.0/270.0 Da Baseline Missed
r Wrnnn Peak
PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFOS 503.0/80.0 Da u Baseline Missed ___n Wrnnn Peak
4.74
}. f
13C-PFDoA 615.0/169.0 Da Baseline a Missed
r- Wrnnn Peak
PFTrA 1 663.0/619.0 Da u Baseline u Missed ___n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da g Baseline Missed ___ n Wrnnn Peak___
:! V v y J .f'i ^ 0 /
PFTeA 1 713.0/669.0 Da g Baseline g Missed ___ p Wrnnn Peak______
13C-PFD0A 615.0/169.0 Da g Baseline Missed ______p W rnnn Peak___
Before After
Page 108 of 242 149
Ion Ratio Confirmation
Created with Anal$W*i4eporter Printed: 14/02/2010 6:41:00 PM
K1000911-015
Before After
Page 109 of 242 150
/ ? C o lu m b ia
4L J Analytical Sarvkasr
p. 15 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name A c q u isitio n Date A cquisition Method Injection Vial
K1000911-015 2/9/2010 9:00:23 AM PFCkinetexShort.dam 55
Injection Volume Sample Type Dilution Factor W eight to Volume
5 Unknown
1.00 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
130-PFHxA
PFHpA 1
13C-PFHXA
PFHxS 1
180-PFHS
S J8 0 -P F H S
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 3432600 4675900 3152600 4675900 4675900 3152600 4149500 4149500
Analyte RT (Exp RT ) 1.27 (1.27) 3.58 (3.60) 4.25 (4.26) 5.29 (5.31) 6.23 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 41844 37860 6043 52858 16153 97998
3152600 4149500
Calc. Cone (ng/mL) 0.06 0.06
0 .0 2
0.05 0.03 \ 0.09
0 .8 6
1.00
Modified? No Mo No No No No No No
i c^
i- * 1
Page 105 of 245 151
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IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp R T ) 1.27 (1.27) 3.58(3.60) 4.25 (4.26) 5.31 (5.31) 6 .2 2 (6 .2 2 ) 6.31 (6.32) 1.27 (1.26) 5.30(5.30)
Area 41844 37860 3068 7597 295190 35772 3432600 4675900
Modified?
Calc (Exp) MRM Ratio
No 1 .0 0 (1 .0 0 )
Ratio Confirms ID
YES
No 1 .0 0 (1 .0 0 )
YES
No 0.51 (0.40)
No .r* lEL-
No 0.14 (0.10)
No
No 18.27(3.03) No 0.37(0.40)
No vf YES
No 82.03 (13.55)
No
No 88.46(13.06)
No
Page 106 of 245 152
p. 17 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Quantitative Peak Review
K1000911-015
- : I . . . .^
i
PFBA 1 213.0/169.0 Da c Baseline Missed r Wronn Peak '"'j
13C-PFBA 217.0/172.0 Da Baseline g Missed n Wrnnn Peak : ; - j 6.31
""i 4.25
.... JL,-.-
PFBS 1 299.0/80.0 Da c Baseline Missed n Wrnnn Peak
6[
;I l
18 0 -P F H S 403.0/103.0 Da u Baseline c Missed n Wrnnn Peak ,.,.j 5.^0
"'l t "l `
,'d
3.58 ____ J L .. - ..
PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Peak
9 5f
: | n ...... m
.........
PFHxAl
313.0/269.0 Da
l Baseline c Missed n Wrnnn Peak
''! 6.31
5^0
13C-PFHxA 315.0/270.0 Da L Baseline Missed i ' Wrnnn Peak
5.3
|
: n-s
13C-PFHxA 315.0/270.0 Da Baseline c Missed n Wrnnn Peak 9. 6 A 1
PFHpA 1 363.0/169.0 Da l Baseline l Missed r Wrnnn Peak .: 6.31
13C-PFHxA
315.0/270.0 Da g Baseline Missed
n Wrnnn Peak - 7.^4
s1
PFHxS1 399.0/80.0 Da Baseline u Missed i ' Wrnnn Peak ' " 1 7.64
L .L
i
I
31
S 180-PFHS
13C-PFDA
403.0/103.0 Da
515.0/470.0 Da
u Baseline Missed
Baseline Missed
_____ r . Wrnnn Peak_____ _____ n Wrnnn Peak_____
S 13C-PFDA 515.0/470.0 Da u Baseline Missed i ; Wrnnn Peak
Before After
180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak
i. I'm1.'
7^
13C-PFDA
515.0/470.0 Da
Baseline Missed
n Wrnnn Peak
Page 107 of 245 153
p. 18 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
K1000911-015
."...jA./vy -vv. ML
3.58 ..Jl.-'..'--- ------
.. 4f .
PFBA 2 213.0/169.0 Da c Baseline u Missed c Wrong Peak
PFPeA 2 263.0/219.0 Da C Baseline Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
6 | 2 :; j I
;:i| ... -
, J | ............. ;
'~.i . . ./V .
..
J i __ A
! E|
\
I L^
PFHpA2 363.0/319.0 Da c Baseline u Missed Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed d Wrong Peak
S 13C-PFDA 515.0/470.0 Da Baseline Missed u Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
; Ik
:.,,l r^ j s . 31
PFHxA2 313.0/119.0 Da Baseline a Missed u Wrong Peak
ThisInage isnot svaiaMe
N/A Baseline Missed
o Wrong Peak
Page 108 of 245 154
p. 19 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrum ent Name
Ewan's ProjectsVPFCs 02081 Ob\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name A c q u is itio n Date A cquisition Method Injection Vial
K1000911-016 2/8/2010 10:47:24 PM PFCkinetexLong.dam 56
Injection Volume Sample Type Dilution Factor W eight to Volume
5 Unknown
1.00 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDoA
IS Area 2227200 2054700 6628100 2632800 112700 6628100 143560 143560 143560
Analyte RT (Exp RT ) 1.37 (1.36) 0.00 (2.42) 2.34 (2.53) 0.00(3.34) 4.12 (4.06) 0.00(4.10) 0.00 (4.63) 0.00(5.14) 0.00 (5.74)
Analyte Area 171870
0
69508
0
7794
0 0 0 0
Calc. Cone (ng/mL) 1.620 N/A 0.113 N/A 0.051 N/A N/A N/A N/A
Modified? Mo Mo No No No No No No No
fy ( ( Ur(
'U
Page 110 of 242 155
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IRC Summary
Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 1.37 (1.36) 1.35(1.36) 2.29 (2.44) 2.29 (2.44) 2.37 (2.53) 2.34(2.53) 0.00(3.34) 3.31 (3.34) 0.00 (4.06) 4.10(4.06) 4.11 (4.10) 4.73 (4.64) 4.75 (4.64) 5.27(5.13) 5.37 (5.56)
Area 361430 2227200
491 2054700
21468 6628100
0
2632800
0
112700 551 1196
143560 354 199
Modified?
Calc (Exp) MRM Ratio
No 2.10(2.38)
Ratio Confirms ID
YES
No 12.96(50.17)
No
No 0 .0 0 (0.28)
No
No 0.00 (19.17)
No
No 0.31 (0.32)
YES
No 95.36 (22.53)
No
No 0.00 (0.15)
No
No 0 .0 0 (2 2 .1 0 )
No
No 0 .0 0 (0 .2 2 )
No
No 14.46(1.86)
No
No 0.00 (0.32)
No
No 0.00 (0.29)
No
No 0.00 (3.08)
No
No 0.00 (0.32)
No
No 0.00 (0.36)
No
Page 111 of 242 156
/ T Columbia w a Analytical Services'
Quantitative Peak Review
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K1000911-016
Before After
157
Page 112 of 242
Ion Ratio Confirmation
1.37
p. 22 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000911-016
PFOA2 413.0/369.0 Da l Baseline Missed c Wrong Peak
PFUnA2 563.0/269.0 Da c Baseline l Missed l Wrong Peak
PFNA2 463.0/219.0 Da c Baseline Missed Wrong Peak
PFOS 2 499.0/99.0 Da c Baseline o Missed Wrong Peak
i; .
PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak
f.on
PFDS2 599.0/99.0 Da G Baseline Missed Wrong Peak
W 4ajiLe4L."v - * 4U *e! $n SS
PFDoA 2 613.0/169.0 Da Baseline c Missed Wrong Peak
PFTrA 2 663.0/169.0 Da 3aseline c Misse q Wrong Peak
PFTeA2 713.0/169.0 Da Baseline d Missed c Wrong Peak
Before After
Page 113 of 242 158
Columbia Arslyikaf services"
p. 23 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
P ro je c t Data File R esult Table Instrum ent Nam e
Ewan's ProjectsVPFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sam ple Nam e A cquisition Date A cquisition M ethod Injection Vial
K1000911-016 2/9/2010 9:09:55 AM PFCkinetexShort.dam 56
Injection Volum e Sam ple Type D ilution Factor W eight to Volum e
5 Unknown
1.00 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
130-PFHxA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHxA
PFHpA 1
13C-PFHxA
PFHxS 1
180-PFHS
S J8 0 -P F H S
13C-PFDA
S J 3C-PFDA
13C-PFDA
IS Area 2405200 3016900 3081200 3016900 3016900 3081200 4358600 4358600
Analyte RT (Exp RT ) 1.27 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 32001 54685 34014 86119 20942 185170
3081200 4358600
Calc. Cone (ng/ml_) 0.07 0.17 0.05
0.21
0 .1 2
0.17 0.80
1.00
Modified? No No No No No No No No
|iC'
Page 109 of 245 159
p. 24 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp R T ) 1.27(1.27) 3.59(3.60) 4.25 (4.26) 5.31 (5.31) 6 .2 2 (6 .2 2 ) 6.31 (6.32) 1.26(1.26) 5.30(5.30)
Area 32001 54685 13650 9677 246540 65974 2405200 3016900
Modified?
Calc (Exp) MRM Ratio
No 1 .0 0 (1 .0 0 )
Ratio Confirms ID
YES
No 1 .0 0 (1 .0 0 )
YES
No 0.40(0.40)
YES
No 0.11 (0 .1 0 )
YES
No 11.77(3.03)
No lA jL l
No 0.36(0.40)
YES
No 75.16(13.55)
No
No 35.03(13.06)
No
Page 110 of 245 160
Quantitative Peak Review
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K1000911-016
Before After
Page 111 of 245 161
Ion Ratio Confirmation
p. 26 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
K1000911-016
PFBA2 213.0/169.0 Da l Baseline c Missed c Wrong Peak
PFPeA2 263.0/219.0 Da Baseline d Missed o Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed o Wrong Peak
PFHxA 2 313.0/119.0 Da Baseline o Missed Wrong Peak
Thisi m p nolavAfcfc
PFHpA2 363.0/319.0 Da L_ Baseline c Missed l Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak
S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
N/A
Baseline Missed Wrong Peak
Page 112 of 245 162
Created with A n a ls t Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrum ent Name
Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name A cqu isitio n Date A cquisition Method Injection Vial
K1000911-017 2/8/2010 10:55:01 PM PFCkinetexLong.dam 57
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown
1 .0 0 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 1980500 1787500 6105700 2639200 118220 6105700 130870 130870 130870
Analyte RT (Exp RT ) 1.39 (1.36) 0.00 (2.42) 2.35 (2.53) 0.00(3.34) 4.12 (4.06) 4.13 (4.10) 0.00 (4.63) 0.00 (5.14) 0.00(5.74)
Analyte Area 145550
0
88469
0
3539 4367
0 0 0
Calc. Cone (ng/mL) 1.550 N/A 0.151 N/A
0 .0 1 0
0.027 N/A N/A N/A
Modified? No No No No No No No No No
,.r/< A
Page 114 of 242 163
p. 28 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_130-PFDoA PFTrA 2 PFTeA2
RT (Exp R T ) 1.38(1.36) 1.38 (1.36) 2.26(2.44) 2.30 (2.44) 2.39 (2.53) 2.37 (2.53) 0.00 (3.34) 3.34 (3.34) 0.00 (4.06) 4.12 (4.06) 4.11 (4.10) 4.73 (4.64) 4.74 (4.64) 5.06(5.13) 5.78 (5.56)
Area 312200 1980500
600 1787500 28901 6105700
0
2639200
0
118220 1169 1331
130870 432 341
Modified? No
Calc (Exp) MRM Ratio
2.14(2.38)
Ratio Confirms ID
YES
No 13.61 (50.17)
No
No 0 .0 0 (0.28)
No
No 0.00(19.17)
No
No 0.33(0.32)
YES
No 69.02(22.53)
No
No 0.00(0.15)
No
No 0 .0 0 (2 2 .1 0 )
No
No 0 .0 0 (0 .2 2 )
No
No 33.40(1.86)
No
No 0.27(0.32)
YES
No 0.00 (0.29)
No
No 0.00(3.08)
No
No 0.00(0.32)
No
No 0.00(0.36)
No
Page 115 of 242 164
p. 29 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Quantitative Peak Review
1.39
1.38
K1000911-017
2.30
i PFOA 1 413.0/169.0 Da Baseline c Missed i - Wrnnn Peak
PFOS 1 499.0/80.0 Da Baseline c Missed n Wrnnn Pnak
13C-PFOA 417.0/372.0 Da u Baseline Missed n Wrnnn Peak
i' 2i 7 13C-PFOS 503.0/80.0 Da Baseline Missed
n Wrnnn Peak
PFUnA 1 563.0/519.0 Da g Baseline Missed r Wrnnn Peak
:
'
. ; :4iu * r-
j,' 4 ^ ^
PFDoA 1 613.0/569.0 Da c Baseline Missed i Wrnnn Peak
13C-PFUnA 565.0/270.0 Da Baseline Missed __ n Wrnnn Peak___
4.74
13C-PFD0A 615.0/169.0 Da Baseline Missed n Wrnnn Peak
PFNA 1 463.0/419.0 Da Baseline d Missed
n Wrnnn Peak
L
13C-PFNA 468.0/423.0 Da g Baseline c Missed n Wrnnn Peak
3.34
*4* : \-i 1 V'w
PFDA 1 513.0/469.0 Da u Baseline c Missed
n Wrnnn Pnak
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Pfiak
2.37
4.13
PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFOS
503.0/80.0 Da u Baseline u Missed
Wrnnn Peak 4.74
.-'V-'ii ' 1
PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak
i_______i ____
13C-PFDOA 615.0/169.0 Da c Baseline Missed ___ n Wrnnn Peak___
PFTeA 1 713.0/669.0 Da l Baseline c Missed ___n Wrnnn Peak___
13C-PFD0A 615.0/169.0 Da a Baseline Missed ___n Wrnnn Peak___
Before After
165
Page 116 of 242
Ion Ratio Confirmation
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000911-017
Before After
Page 117 of 242 166
Created with A n a lyif Reporter Printed: 15/02/2010 2 34:00 PM
P ro je c t D ata File R esult Table Instrum ent Nam e
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sam ple Nam e A cquisition Date A cquisition M ethod Injection Vial
K1000911-017 2/9/2010 9:19:28 AM PFCkinetexShort.dam 57
Injection Volum e Sam ple Type Dilution Factor W eight to Volum e
5 Unknown
1.00 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
130-PFHxA
PFHpA 1
13C-PFHXA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
SJ3C-PFDA
13C-PFDA
l__________________
IS Area 2478600 3024600 2902700 3024600 3024600 2902700 4680100 4680100
Analyte RT (Exp RT ) 1.26 (1.27) 3.58 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65)
Analyte Area 35923 40217 31747 84373 20716 180790
2902700 4680100
Calc. Cone (ng/mL) 0.08
0 .1 2
0.05
0.21
0 .1 2
0.18 0.70
1.00
Modified? No No No No No No No No
A" *
Page 113 of 245 167
Created with Anal^sfReporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp R T ) 1.26 (1.27) 3.58(3.60) 4.25 (4.26) 5.30(5.31) 6 .2 0 (6 .2 2 ) 6.31 (6.32) 1.26(1.26) 5.30 (5.30)
Area 35923 40217 11811 7290 74802 66014 2478600 3024600
Modified? No
Calc (Exp) MRM Ratio
1 .0 0 (1 .0 0 )
Ratio Confirms ID
YES
No 1 .0 0 (1 .0 0 )
YES
No 0.37(0.40)
YES
No 0.09(0.10)
YES
No 3.61 (3.03)
YES
No 0.37 (0.40)
YES
No 69.00 (13.55)
No
No 35.85 (13.06)
No
Page 114 of 245 168
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Before After
Page 115 of 245 169
p. 34 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
K1000911-017
,.:a
PFBA2 213.0/169.0 Da c Baseline Missed u Wrong Peak
6^0
PFPeA2 263.0/219.0 Da g Baseline c Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
!5f.
. a. ; : : : ':
......... 6.31_____ _ i '*'* is
ufl I T :i)
PFHpA 2 363.0/319.0 Da Baseline Missed Wrong Peak
PFHxS2 399.0/99.0 Da a Baseline Missed Wrong Peak
S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
PFHxA 2 313.0/119.0 Da c Baseline a Missed Wrong Peak
Thisinageisnolavaiable
N/A Baseline Missed
Wrong Peak
Page 116 of 245 170
p. 35 Created with Analyst Reporter
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P ro je c t D ata File R esult Table Instrum ent Nam e
Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sam ple Nam e A cquisition Date A cquisition M ethod Injection Vial
K1000911-018 2/8/2010 11:02:36 PM
PFCkinetexLong.dam
58
Injection Volum e Sam ple Type Dilution Factor W eight to Volum e
5
Unknown
1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3103100 2075500 5243700 2376700
96754 5243700 108200 108200 108200
Analyte RT (Exp RT ) 1.39 (1.36) 2.32 (2.42) 2.37 (2.53) 3.34 (3.34) 4.10 (4.06) 4.11 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74)
Analyte Area 232070 36650 1767900 22682 6862 20200 0 0 0
Calc. Cone
(ng/m L)
1.570 0.178 3.180 0.119 0.053 0.053 N/A N/A N/A
Modified? No No No No No No No No No
,u ( ' c Z -/
Page 118 of 242 171
p. 36 Created with Anafyst Reporter Printed: 14/02/2010 6:41:00 PM
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT) 1.40(1.36) 1.39 (1.36) 2.32(2.44) 2.34 (2.44) 2.37(2.53) 2.36 (2.53) 0.00(3.34) 3.32 (3.34) 0.00 (4.06) 4.11 (4.06) 4.13 (4.10) 4.74 (4.64) 4.75 (4.64) 5.32(5.13) 5.34 (5.56)
Area 467030 3103100 10898 2075500 532070 5243700
0 2376700
0 96754 7486 1494 108200
460 252
Modified? No
Calc (Exp) MRM Ratio
2.01 (2.38)
Ratio Confirms ID
YES
No 13.37(50.17)
No
No 0.30(0.28)
YES
No 56.63(19.17)
No
No 0.30(0.32)
YES
No 2.97(22.53)
No
No 0.00 (0.15)
No
No 104.78 (22.10)
No
No 0.00(0.22)
No
No 14.10(1.86)
No
No 0.37 (0.32)
YES
No 0.00 (0.29)
No
No 0.00(3.08)
No
No 0.00(0.32)
No
No 0.00 (0.36)
No
Page 119 of 242 172
Columbia Analytical Services
Quantitative Peak Review
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K1000911-018
Before After
173
Page 120 of 242
Ion Ratio Confirmation
Created with Analty$?&eporter Printed: 14/02/2010 6:41:00 PM
K1000911-018
Before After
Page 121 of 242 174
/ ? Columbia Analytical Services
p. 39 Created with Analysi Reporter
Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrum ent Name
Ewan's Projects\PFCs 02081 OXSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name A cquisition Date A cquisition Method Injection Vial
K1000911-018 2/9/2010 9:29:03 AM PFCkinetexShort.dam 58
Injection Volume Sample Type Dilution Factor W eight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
130-PFHxA
PFBS 1
18 0 -P F H S
PFHxA 1
13C-PFHxA
PFHpA 1
130-PFHxA
PFHxS 1
180-PFHS
S J8 0 -P F H S
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 3905700 5026500 3180100 5026500 5026500 3180100 4170300 4170300
Analyte RT (Exp RT ) 1.26 (1.27) 3.60 (3.60) 4.26 (4.26) 5.31 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65)
Analyte Area 156640 260310 245730 316170 93061 1309100 3180100 4170300
Calc. Cone (ng/mL) 0.32 0.52 0.24 0.53 0.42 1.23 0.87 1.00
Modified? No No No No No No No No
r y c '
Page 117 of 245 175
/ ? C o lu m b ia
L j Analytical S-m m cm *
p. 40 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT) 1.26 (1.27) 3.60 (3.60) 4.25(4.26) 5.30(5.31) 6.22 (6.22) 6.31 (6.32) 1.26 (1.26) 5.30(5.30)
Area 156640 260310 92190 27049 300620 497560 3905700 5026500
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.38(0.40)
YES
No 0.09(0.10)
YES
No 3.23(3.03)
YES
No 0.38(0.40)
YES
No 24.93 (13.55)
No
No 15.90(13.06)
No
Page 118 of 245 176
p. 41 Created with Analyst Reporter
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Quantitative Peak Review
126
K1000911 -018
Vi ,*p 5`^
PFBA 1 213.0/169.0 Da c Baseline l Missed i Wrnnn Peak
4^
PFBS 1 299.0/80.0 Da
c Baseline c Missed r. Wronn Peak
: 6.22
11
13C-PFBA 217.0/172.0 Da c Baseline Missed n Wronn Peak
I
PFPeA 1 263.0/219.0 Da Baseline c Missed
n Wrnnn Peak
:^ I
180-PFHS 403.0/103.0 Da u Baseline Missed _____ n Wrnnn Peak_____
LO
PFHxA 1 313.0/269.0 Da G Baseline o Missed r Wrnnn Peak_____
J
13C-PFHXA 315.0/270.0 Da Baseline c Missed n Wrnnn Peak
13C-PFHXA 315.0/270.0 Da u Baseline Missed p Wrnnn Peak
C' 6.^1
""J cn
/L
PFHpA 1 363.0/169.0 Da u Baseline p Missed p Wronn Peak
6.31
13C-PFHxA
315.0/270.0 Da
u Baseline c Missed
n Wrnnn Peak
PFHxS 1 399.0/80.0 Da U Baseline Missed p Wrnnn Peak
18 0 -P F H S
403.0/103.0 Da
l Baseline Missed i >Wrnnn Peak
j 7.^5
:-
1l
i
S 180-PFHS
13C-PFDA
403.0/103.0 Da
515.0/470.0 Da
u Baseline Missed
u Baseline Missed
_____ i : Wrong Peak_____ _____ ri Wrnnn Peak_____
S 13C-PFDA
13C-PFDA
515.0/470.0 Da
515.0/470.0 Da
c Baseline l Missed
u Baseline Missed
r Wrnnn Peak_____ _____ n Wronn Peak_
Before After
Page 119 of 245 177
S ervices"
p. 42 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
1.26
K1000911-018
_______ __
,J|__
M - ` 4.j 5
PFBA 2 213.0/169.0 Da Baseline a Missed c Wrong Peak
: 6.22
PFPeA 2 263.0/219.0 Da Baseline u Missed o Wrong Peak
-! I
PFBS2 299.0/99.0 Da g Baseline Missed u Wrong Peak
,0J .. . -
7 -
/ '1
::<
........-
,,, ......
l
PFHp 2 363.0/319.0 Da g Baseline Missed g Wrong Peak
PFHxS 2 399.0/99.0 Da Baseline Missed Wrong Peak
S 13C-PFDA 515.0/470.0 Da g Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
:3 I L_
PFHxA2 313.0/119.0 Da Baseline l Missed c Wrong Peak
Thismap rriavble
N/A g Baseline g Missed
Wrong Peak
Page 120 of 245 178
p. 43 Created with Analysl Reporter
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P ro je c t D ata File R esult Table Instrum ent Nam e
Ewan's Projects\PFCs 02081 Ob\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000
Sam ple Nam e A cquisition Date A cquisition M ethod Injection Vial
K1000911-019 2/8/2010 11:10:10 PM PFCkinetexLong.dam 59
Injection Volum e Sam ple Type D ilution Factor W eight to Volum e
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3138700 2375900 5901000 2419900
96973 5901000 122360 122360 122360
Analyte RT
(Exp RT )
1.34 (1.36) 0.00 (2.42) 2.32 (2.53) 0.00 (3.34) 4.10 (4.06) 4.10 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74)
Analyte Area 8053 0 50829 0 4308 2434 0 0 0
Calc. Cone
(ng/m L)
0.087 N/A 0.095 N/A 0.025 0.024 N/A N/A N/A
Modified? No No No No No No No No No
nr
S ', Y 7
Page 122 o f 242 179
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
m s*
p. 44 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
RT (Exp RT) 1.32(1.36) 1.34(1.36) 2.36 (2.44) 2.28 (2.44) 2.34 (2.53) 2.33 (2.53) 0.00 (3.34) 3.30 (3.34) 0.00 (4.06) 4.09 (4.06) 4.11 (4.10) 4.73 (4.64) 4 .7 4 (4.64) 5.26(5.13) 5.74 (5.56)
Area 19968 3138700 450 2375900 16101 5901000
0 2419900
0 96973
502 1626 122360 933 362
Modified? No
Calc (Exp) MRM Ratio
2.48(2.38)
Ratio Confirms ID
YES
No 389.76(50.17)
No
No 0.00(0.28)
No
No 0.00(19.17)
No
No 0.32(0.32)
YES
No 116.10(22.53)
No
No 0.00 (0.15)
No
No 0.00 (22.10)
No
No 0.00 (0.22)
No
No 22.51 (1.86)
No
No 0.21 (0.32)
No
No 0.00 (0.29)
No
No 0.00 (3.08)
No
No 0.00 (0.32)
No
No 0.00 (0.36)
No
Page 123 of 242 180
JT l C o lu m b ia
Analytical Services''
Quantitative Peak Review
1.34
p. 45 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000911-019
PFOA 1 413.0/169.0 Da c Baseline Missed i Wronn Peak
PFOS 1 499.0/80.0 Da Baseline u Missed n Wrnnn Peak
4.10
PFUnA 1 563.0/519.0 Da Baseline u Missed r Wrnnn Peak I
L ; ;'C,: 4/ \ , f 0 l
PFDoA 1 613.0/569.0 Da Baseline g Missed r Wrnnn Peak
13C -P F O A 417.0/372.0 Da Baseline Missed 11 Wrnnn Peak
13C-PFOS 503.0/80.0 Da u Baseline Missed n Wrnnn Peak
13C-PFUnA 565.0/270.0 Da o Baseline g Missed ___ n Wronn Peak
13C-PFDoA 615.0/169.0 Da Baseline c Missed r: Wrnnn Peak
I ' . i.'A
PFNA 1 463.0/419.0 Da u Baseline Missed n Wrnnn Peak
i
il
!V v'v!rC Z ; '!/; `
PFDA 1 513.0/469.0 Da Baseline c Missed n Wrnnn Peak
4.10
PFDS 1 599.0/80.0 Da u Baseline u Missed ___ n Wronn Peak
13C-PFNA 468.0/423.0 Da Baseline Missed r Wronn Peak
13C-PFDA 515.0/470.0 Da Baseline c Missed r Wrnnn Peak
:u
L
13C-PFOS 503.0/80.0 Da Baseline u Missed ___ n Wronn Peak ___
4.74
'vYv' k i-
PFTrA 1 663.0/619.0 Da Baseline c Missed ___ r Wrnnn Peak___
13C-PFDoA 615.0/169.0 Da Baseline g Missed ___ r Wrnnn Peak___
PFTeA 1 713.0/669.0 Da
g Baseline c Missed i Wrnnn Peak
13C-PFDoA 615.0/169.0 Da
_c___Bra sWerlinnnencPMeaisksed
Before After
181
Page 124 of 242
Created with A nalfst4$eporter Printed: 14/02/2010 6:41:00 PM
Ion Ratio Confirmation
7 ' 1.32 :V
/ ..i
K1000911-019
s
i-
wmi!
\
Ha A
PFOA2 413.0/369.0 Da l Baseline Missed l Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak
PFOS2 499.0/99.0 Da c Baseline Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak
PFUnA2 563.0/269.0 Da l Baseline Missed Wrong Peak
PFDS 2 599.0/99.0 Da Baseline Missed d Wrong Peak
PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak
'W
PFTeA2 713.0/169.0 Da lj Baseline Missed Wrong Peak
Before After
Page 125 of 242 182
/ ? C o lu m b ia
Analytics! S e r v a is*
p. 47 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
P ro je c t Data File R esult Table Instrum ent Nam e
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sam ple Nam e A cquisition Date A cquisition M ethod Injection Vial
K1000911-019 2/9/2010 9:38:35 AM PFCkinetexShort.dam 59
Injection Volum e Sam ple Type Dilution Factor W eight to Volum e
5 Unknown
1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHxA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHxA
PFHpA 1
13C-PFHxA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 3763200 4768300 3463800 4768300 4768300 3463800 4549400 4549400
Analyte RT
(Exp RT )
1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 115610 69518 21603 101400 19991 90995 3463800 4549400
Calc. Cone
(ng/m L)
0.23 0.13 0.04 0.14
0.07
0 .8 6 1.00
Modified? Mo M'o Mo Mo Mo No No No
i '2 ( i b (L O
Page 121 of 245 183
p. 48 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS 2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT ) 1.26(1.27) 3.59 (3.60) 4.25(4.26) 5.30(5.31) 6 .2 1 (6 .2 2 ) 6.31 (6.32) 1.25 (1.26) 5.30(5.30)
Area 115610 69518 7942 9080 371810 36014 3763200 4768300
Modified? No
Calc (Exp) MRM Ratio
1 .0 0 (1 .0 0 )
Ratio Confirms ID
YES
No 1 .0 0 (1 .0 0 )
YES
No 0.37(0.40)
YES
No 0.09(0.10)
YES
No 18.60 (3.03)
No / MiLt
No 0.40(0.40)
YES
No 32.55 (13.55)
No
No 47.02(13.06)
No
Page 122 of 245 184
/ticai Strv'ices Quantitative Peak Review
PFBA 1 213.0/169.0 Da
Baseline Missed r Wronn Peak
4.?5
13C-PFBA 217.0/172.0 Da Baseline Missed 11 Wrnnn Peak
6.3
p. 49 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
K1000911-019
5.30
-4 3 59
j . .A . - -
PFPeA 1 263.0/219.0 Da c Baseline Missed n Wrnnn Peak
13C-PFHxA 315.0/270.0 Da Baseline Missed r. Wrnnn Peak
PFBS 1 299.0/80.0 Da g Baseline u Missed n Wrnnn Peak
180-PFHS 403.0/103.0 Da c Baseline Missed n Wrnnn Peak
____
p f h x a 'i 313.0/269.0 Da Baseline c Missed n Wrnnn Peak
13C-PFHxA 315.0/270.0 Da Baseline g Missed n Wrnnn Peak
PFHpA 1 363.0/169.0 Da Baseline g Missed i Wrnnn Peak
13C-PFHxA 315.0/270.0 Da c Baseline Missed
n Wrnnn Peak
PFHxS 1 399.0/80.0 Da Baseline c Missed r Wrnnn Peak
180-PFHS 403.0/103.0 Da u Baseline u Missed
n Wrnnn Peak
S_180-PFHS 403.0/103.0 Da g Baseline c Missed r Wrnnn Peak
13C-PFDA 515.0/470.0 Da g Baseline g Missed ___p Wrnnn Peak___
Before After
SJ3C-PFDA 515.0/470.0 Da Baseline g Missed 11 Wrnnn Peak___
13C-PFDA 515.0/470.0 Da Baseline Missed ___r ! Wrnnn Peak___
Page 123 of 245 185
p. 50 Created with Analyst Reporter
bis Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
1.26
i M L ._ _
PFBA 2 213.0/169.0 Da d Baseline o Missed u Wrong Peak
5J .......
3.59
PFPeA2
263.0/219.0 Da g Baseline c Missed
Wrong Peak
K1000911-019
: - . . . . : ____ .4 . 5 . . .. .: _____
ij i
I M L oW -W '
PFBS 2
299.0/99.0 Da Baseline u Missed
c Wrong Peak
PFHxA 2
313.0/119.0 Da Baseline u Missed
Wrong Peak
'.r.i"' . /v....
*.S ' -5U
'0
ii r
PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak
PFHxS2 399.0/99.0 Da c Baseline u Missed Wrong Peak
S 13C-PFDA 515.0/470.0 Da Baseline c Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
T/k triageisnotavaiable
N/A g Baseline Missed
Wrong Peak
Page 124 of 245 186
p. 51 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrum ent Name
Ewan's Projects\PFCs 02081 OtSamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name A cquisition Date Acquisition Method Injection Vial
KQ1000532-04 2/9/2010 10:07:18 AM PFCkinetexShort.dam 62
^
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown
1.00 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
130-PFHxA
PFHpA 1
13C-PFHXA
PFHxS1
180-PFHS
S_180-PFHS
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 4940500 5543000 3134300 5543000 5543000 3134300 4457300 4457300
Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.31 (5.31) 6.22 (6.23) 6.31 (6.32) 6.30 (6.31) 7.64 (7.65)
Analyte Area 57792 73994 10230 64231 16836 52991
3134300 4457300
Calc. Cone (ng/mL) 0.05
0 .1 2
0.03 0.05
0.02
0.04 0.80
1.00
Modified? No No No No No No No No
y\
Page 133 of 245 187
Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
l Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT ) 1.26(1.27) 3.59 (3.60) 4.26 (4.26) 0.00 (5.31) 6 .2 2 (6 .2 2 ) 6.31 (6.32) 1.26(1.26) 5.30(5.30)
Area 57792 73994 4317
0
224960 18134 4940500 5543000
Modified?
Calc (Exp) MRM Ratio
No 1 .0 0 (1 .0 0 )
Ratio Confirms ID
YES
No 1 .0 0 (1 .0 0 )
YES
No 0.42(0.40)
YES
No 0 .0 0 (0 .1 0 )
No
No 13.36 (3.03)
No
No 0.34(0.40)
YES
No 85.49(13.55)
No
No 86.30(13.06)
No
Page 134 of 245 188
p. 53 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Quantitative Peak Review
KQ1000532-04
PFBA 1 213.0/169.0 Da Baseline Missed r Wronn Peak
4.25
PFBS 1 299.0/80.0 Da Baseline Missed n Wrnnn Peak
6.22
13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak
180-PFHS 403.0/103.0 Da d Baseline Missed r Wrnnn Peak
3.59 ...Jl . PFPeA 1 263.0/219.0 Da a Baseline Missed n Wrnnn Peak 5.81
PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnno Peak _
13C-PFHxA 315.0/270.0 Da Baseline a Missed r Wrnnn Peak
13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak
1
PFHpA 1 363.0/169.0 Da Baseline Missed n Wrnnn Peak
13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak
PFHxS 1 399.0/80.0 Da Baseline c Missed n Wrnnn Peak
ecir,
180-PFHS 403.0/103.0 Da Baseline o Missed p Wronn Peak
S J8 0 -P F H S 403.0/103.0 Da Baseline o Missed ___ p Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed r Wrnnn Peak
S J3C -P F D A 515.0/470.0 Da g Baseline c Missed ___ n Wrnnn Peak
Before After
13C-PFDA 515.0/470.0 Da Baseline a Missed r Wrnnn Peak
Page 135 of 245 189
Created with Anal^fstReporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
KQ1000532-04
PFBA2 213.0/169.0 Da Baseline Missed c Wrong Peak
3.59
A
v,
PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed c Wrong Peak
PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak
Thisinage ii not aralable
PFHpA2 363.0/319.0 Da Baseline Missed c Wrong Peak
PFHxS2 399.0/99.0 Da Baseline a Missed Wrong Peak
SJ3C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
N/A
Baseline Missed Wrong Peak
Page 136 of 245 190
Created with AnalysPpeporter & Printed: 14/02/2010 6:41:00 PM
P ro je c t D ata File R esult Table Instrum ent Nam e
Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sam ple Nam e A cqu isitio n Date A cquisition M ethod Injection Vial
K Q 1000532-04 2/8/2010 11:32:52 PM
PFCkinetexLong.dam
62
/IP
Injection Volum e Sam ple Type Dilution Factor W eight to Volum e
5
Unknown
1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
130-PFDoA
IS Area 3286600 2543200 6352500 2564200 134510 6352500 143760 143760 143760
Analyte RT (ExpRT) 0.00 (1.36) 0.00(2.42) 2.32 (2.53) 0.00 (3.34) 4.09 (4.06) 4.12 (4.10) 4.75 (4.63) 0.00(5.14) 0.00 (5.74)
Analyte Area 0 0
34857
0
7404 17556 6189
0 0
Calc. Cone (ng/m L) N/A N/A 0.066 N/A 0.036 0.044 0.045 N/A N/A
Modified? No No No No No No No No No
-7 ( l ^ i L
Page 134 of 242 191
C o lu m b ia
O Analytical Services'-
Createci with AnalysP'Feporter Printed: 14/02/2010 6:41:00 PM
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFD0A PFTrA 2 PFTeA2
RT(ExpRT) 1.35 (1.36) 1.36(1.36) 2.26 (2.44) 2.29 (2.44) 2.33 (2.53) 2.33 (2.53) 0.00 (3.34) 3.31 (3.34) 0.00 (4.06) 4.10 (4.06) 4.12 (4.10) 4.73 (4.64) 4.73 (4.64) 5.28 (5.13) 5.74(5.56)
Area 9693 3286600 835 2543200 11713 6352500
0
2564200
0
134510 4513 2080 143760 354 123
Modified?
Calc (Exp) MRM Ratio
No 0.00 (2.38)
Ratio Confirms ID
No
No 0.00(50.17)
No
No 0 .0 0 (0.28)
No
No 0.00 (19.17)
No
No 0.34(0.32)
YES
No 182.24 (22.53)
No
No 0.00 (0.15)
No
No 0 .0 0 (2 2 .1 0 )
No
No 0 .0 0 (0 .2 2 )
No
No 18.17(1.86)
No
No 0.26 (0.32)
YES
No 0.34(0.29)
YES
No 23.23(3.08)
No
No 0.00 (0.32)
No
No 0.00 (0.36)
No
Page 135 of 242 192
jT i C ^ U R lb ia
J S Analytical Services"
Quantitative Peak Review
1 36
PFOA 1 413.0/169.0 Da c Baseline Missed r Wrnnn Peak
O*50
:1
PFOS 1 499.0/80.0 Da u Baseline Missed n Wrnnn Peak
m
;i! i13C-PFOA 417.0/372.0 Da q Baseline Missed r Wrnnn Peak
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak ...| 4^0
Created with Analyst fcJporter Printed: 14/02/2010 6:4" :00 PM
IKQ1000532-04 ;
i'
PFNA 1 463.0/419.0 Da c Baseline c Missed n Wrnnn Peak
l mmi
} IJ"'| ,
;
"4 "'':"' p
, r ' '`J
PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak
13C-PFNA 468.0/423.0 Da c Baseline c Missed n Wrnnn Peak
3.31
i
13C-PFDA 515.0/470.0 Da Baseline Missed i ' Wrnnn Peak
2^
Ai
PFUnA 1 563.0/519.0 Da lj Baseline c Missed <- Wrnnn Peak
'.j 4.75
13C-PFUnA 565.0/270.0 Da u Baseline Missed n Wrnnn Peak -4 4.73
PFDS 1 599.0/80.0 Da l Baseline Missed n Wrnnn Peak
:
PFDoA 1 613.0/569.0 Da e Baseline a Missed r- Wrnnn Peak
i: [
13C-PFDOA 615.0/169.0 Da o Baseline Missed p Wrnnn Peak
PFTrA 1 663.0/619.0 Da Baseline Missed _____ n Wrnnn Peak_____
13C-PFOS 503.0/80.0 Da c Baseline c Missed r Wrnnn Peak
4 |3
1
13C-PFD0A 615.0/169.0 Da c Baseline Missed n Wrnnn Peak
PFTeA 1
13C-PFDoA
713.0/669.0 Da
615.0/169.0 Da
l Baseline c Missed
d Baseline c Missed
r Wronn Peak_____ I_____ Wrnnn Peak_______
Before After
193
Page 136 of 242
f % Columbia Analytical Services1
p. 58 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Ion Ratio Confirmation
KQ1000532-04
s .. 1.35 *
. H
213B
I / " BB88l,\
i ::!
A
i
PFOA2 413.0/369.0 Da c Baseline Missed c Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak
PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed d Wrong Peak
PFUnA2 563.0/269.0 Da Baseline c Missed Wrong Peak
PFDS 2 599.0/99.0 Da Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da Baseline c Missed Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline c Missed c Wrong Peak
PFTeA2 713.0/169.0 Da c Baseline Missed c Wrong Peak
Before After
Page 137 of 242 194
f i Columbia
C J S A n a ly tic a l S ervices"
Created with Analystfaeporter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrum ent Name
Ewan's ProjectsVPFCs 02081 ObVSamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name A cqu isitio n Date Acquisition Method Injection Vial
KQ1000532-05
LC 5
2/8/2010 11:17:45 PM
PFCkinetexLong.dam
60
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown
1.00 0 .0 0
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA1
130-PFDoA
PFTrA 1
130-PFDoA
PFTeA 1
13C-PFDOA
IS Area 3152100 2636500 6337500 2560600 131220 6337500 142110 142110 142110
Analyte RT (Exp RT ) 1.37 (1.36) 2.31 (2.42) 2.35 (2.53) 3.33 (3.34) 4.11 (4.06) 4.12 (4.10) 4.75 (4.63) 5.28 (5.14) 5.73 (5.74)
Analyte Area 2911500 5762100 13202000 4633500 2628600 8543700 1576800 797260 780880
Calc. Cone (ng/mL) 19.000 19.100 19.600 17.200
2 1 .2 0 0
1 2 .0 0 0
20.400 15.900 20.800
Modified? No No No No No No No No No
A
[ID
/p
Page 126 of 242 195
/ ? C o lu m b ia A n a lytica l
Created with Analyit'Reporter Printed: 14/02/2010 6:41:00 PM
IRC Summary
i Analyte Peak Name PFOA 2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 1.37(1.36) 1.35 (1.36) 2.33 (2.44) 2.32 (2.44) 2.35 (2.53) 2.35(2.53) 3.31 (3.34) 3.31 (3.34) 4.11 (4.06) 4.12(4.06) 4.12 (4.10) 4.76 (4.64) 4.75 (4.64) 5.28(5.13) 5.73(5.56)
Area 6889000 3152100 1679600 2636500 4229100 6337500 699450 2560600 581530 131220 2744500 456750 142110 263580 275250
Modified?
Calc (Exp) MRM Ratio
No 2.37 (2.38)
Ratio Confirms ID
YES
No 1.08 (50.17)
No
No 0.29(0.28)
YES
No 0.46(19.17)
No
No 0.32(0.32)
YES
No 0.48 (22.53)
No
No 0.15(0.15)
YES
No 0.55 (22.10)
No
No 0.22(0.22)
YES
No 0.05(1.86)
No
No 0.32(0.32)
YES
No 0.29(0.29)
YES
No 0.09 (3.08)
No
No 0.33(0.32)
YES
No 0.35 (0.36)
YES
Page 127 of 242 196
,/*if
l L 3 Analytical Services"
Created with AnalysPifeporter Printed: 14/02/2010 6A1:00 PM
Quantitative Peak Review
\i 5
- t >*$
!
PFOA1 413.0/169.0 Da Baseline Missed p Wrnnn Peak
O'. Hi : t . Il
13C-PFOA 417.0/372.0 Da c Baseline Missed n Wrnnn Peak
w> 2 ^
KQ1000532-05
|
I H. ;tl
10
PFNA 1 463.0/419.0 Da Baseline Missed p Wrnnn Peak
3 ^3
l
13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak
3^
.-
.'i. i-
PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak
:c Hi
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak
PFDA 1 513.0/469.0 Da Baseline Missed 11 Wrnnn Peak
PFUnA 1 563.0/519.0 Da
u Baseline Missed n Wrnnn Peak
,, j 4.75
I
13C-PFUnA 565.0/270.0 Da Baseline c Missed n Wrnnn Peak
4.^5
if-4
e >&1
Ci.r,
4^
PFDS 1 599.0/80.0 Da c Baseline Missed n Wrnnn Peak . 0*'. 5.^8
13C-PFDA 515.0/470.0 Da Baseline Missed p Wrnnn Peak
,35
Sr--,
1
13C-PFOS 503.0/80.0 Da C Baseline Missed n Wrnnn Peak
4.^5
PFDoA 1 613.0/569.0 Da Baseline o Missed p Wrnnn Peak j 5.73
_j
'" I , L
PFTeA 1 713.0/669.0 Da c Baseline Missed r Wrnnn Peak
13C-PFDOA
615.0/169.0 Da
L Baseline Missed n Wrnnn Peak 4.^5
PFTrAI 663.0/619.0 Da Baseline q Missed p. Wrnnn Peak_____
; ifj
13C-PFDOA 615.0/169.0 Da C Baseline Missed p Wrnnn Peak_____
Before After
13C-PFDoA 615.0/169.0 Da c Baseline Missed _n Wrnnn Peak
Page 128 of 242 197
jT i Columbia
* 3 A n a lytica l Services"
Ion Ratio Confirmation
Created with AnalysPR*eporter Printed: 14/02/2010 6:41:00 PM
KQ1000532-05
PFOA2 413.0/369.0 Da Baseline Missed c Wrong Peak
PFUnA2 563.0/269.0 Da Baseline c Missed Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak
PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak
i. .. .... 4. 12........ . H ~ <0 .<.5.........S.ti
PFDS2 599.0/99.0 Da Baseline o Missed o Wrong Peak
. ,*5
--4,
PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline Missed c Wrong Peak
Before After
Page 129 of 242 198
p. 63 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrum ent Name
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name A cquisition Date Acquisition Method Injection Vial
KQ1000532-05
LC $
2/9/2010 9:48:11 AM
PFCkinetexShort.dam
60
Injection Volume Sample Type Dilution Factor W eight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHxA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHxA
PFHpA 1
13C-PFHXA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 4885800 5625300 3191100 5625300 5625300 3191100 4359600 4359600
Analyte RT (Exp RT ) 1.27 (1.27) 3.59 (3,60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 10205000 10578000 18405000 10749000 4275400 17590000 3191100 4359600
Calc. Cone (ng/mL) 19.70 19.70 18.10 19.00 19.70 18.50 0.83 1.00
Modified? No No No No No No No No
ty p * ?
'( 3
-zJ l
Page 125 of 245 199
p. 64 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT) 1.27(1.27) 3.59(3.60) 4.25 (4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.26 (1.26) 5.30 (5.30)
Area 10205000 10578000 7547600 1021600 12331000 7293000 4885800 5625300
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.41 (0.40)
YES
No 0.10(0.10)
YES
No 2.88(3.03)
YES
No 0.41 (0.40)
YES
No 0.48(13.55)
No
No 0.52(13.06)
No
Page 126 of 245 200
$ r k 5 `
Quantitative Peak Review
p. 65 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000532-05
Before After
Page 127 of 245 201
C<lSiJtYli3i*l
Lm Ji Analytical Services"
Ion Ratio Confirmation
1.27
3.59
Created with A n a ly it Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000532-05
PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak
6.21
PFPeA2 263.0/219.0 Da d Baseline d Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
BM
6'J*5
55 50 5 76
PFHpA 2 363.0/319.0 Da d Baseline Missed c Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed d Wrong Peak
S_13C-PFDA 515.0/470.0 Da a Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
PFHxA 2 313.0/119.0 Da a Baseline Missed Wrong Peak
This maqe is not ayaiable
N/A Baseline Missed
Wrong Peak
Page 128 o f 245 202
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P ro je c t D ata File R esult Table Instrum ent Nam e
Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sam ple Nam e A cquisition Date A cquisition M ethod Injection Vial
KQ1000532-06
DpA)
2/8/2010 11:25:20 PM
PFCkinetexLong.dam
61
Injection Volum e Sam ple Type D ilution Factor W eight to Volum e
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFD0A
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 2974900 2493800 6108000 2412900 139300 6108000 133470 133470 133470
Analyte RT (Exp RT ) 1.38 (1.36) 2.32 (2.42) 2.34 (2.53) 3.30 (3.34) 4.10 (4.06) 4.11 (4.10) 4.75 (4.63) 5.27 (5.14) 5.72 (5.74)
Analyte Area 2942100 5012000 12744000 4356100 2450600 8366400 1562200 885070 702240
Calc. Cone (ng/m L) 20.400 17.600 19.600 17.100 18.600 12.200 21.500 18.800 19.900
Modified? No No No No No No No No No
iu o-
Page 130 of 242 203
Created with AnalysPlR*eporter Printed: 14/02/2010 6:41:00 PM
IRC Summary
Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp R T ) 1.36(1.36) 1.38(1.36) 2.30 (2.44) 2.29 (2.44) 2.34 (2.53) 2.34 (2.53) 3.31 (3.34) 3.31 (3.34) 4.11 (4.06) 4.11 (4.06) 4.11 (4.10) 4.74 (4.64) 4.74 (4.64) 5.27(5.13) 5.72 (5.56)
Area 6585700 2974900 1595000 2493800 4022000 6108000 627100 2412900 562390 139300 2634800 434820 133470 31.8130 265680
Modified?
Calc (Exp) MRM Ratio
No 2.24(2.38)
Ratio Confirms ID
YES
No 1.01 (50.17)
No
No 0.32(0.28)
YES
No 0.50(19.17)
No
No 0.32(0.32)
YES
No 0.48 (22.53)
No
No 0.14(0.15)
YES
No 0.55 (22.10)
No
No 0.23 (0.22)
YES
No 0.06 (1.86)
No
No 0.31 (0.32)
YES
No 0.28(0.29)
YES
No 0.09 (3.08)
No
No 0.36(0.32)
YES
No 0.38(0.36)
YES
Page 131 of 242 204
,/T C o lu m b ia
Analytical Srke$w
Quantitative Peak Review
i 1.38
p. 69 Created with Analysl Reporter Printed: 14/02/2010 6:41:00 PM
KQ1000532-06
i 2.32
l
PFOA 1 413.0/169.0 Da Baseline Missed n Wrnnn Peak
2.34
1
PFOS 1 499.0/80.0 Da Baseline Missed p Wrnnn Peak
t13C-PFOA
417.0/372.0 Da Baseline Missed ___n Wrnnn Peak___ ' 2,34
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak
4^1
PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak
PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak
4.11
13C-PFNA 468.0/423.0 Da c Baseline Missed n Wrnnn Peak i 3.31
1
13C-PFDA 515.0/470.0 Da Baseline Missed p Wrnnn Peak
PFUnA 1 563.0/519.0 Da Baseline o Missed r Wrnnn Peak
' 1 401 J'.
13C-PFUnA
PFDS 7
565.0/270.0 Da
599.0/80.0 Da
Baseline Missed
d Baseline Missed
p Wrnnn Peak_____ _____ n Wrnnn Peak
*.ti
13C-PFOS 503.0/80.0 Da 11
Wrnnn Peak
PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak
PFTrA 1 663.0/619.0 Da d Baseline Missed __ p Wrnnn Peak
13C-PFDoA 615.0/169.0 Da c. Baseline Missed ___ p Wrnnn Peak
PFTeA 1 713.0/669.0 Da c Baseline Missed n Wrnnn Paak
13C-PFDOA 615.0/169.0 Da Baseline Missed ___ p.W rong Peak___
Before After
205
Page 132 of 242
/ "# COIIFr IjS
**J Analytical Services"
Createci with AnalysPReporter Printed: 14/02/2010 6:41:00 PM
Ion Ratio Confirmation
............1,36.............. i:
A
"'I - j j f c - - - -
PFOA2 413.0/369.0 Da u Baseline g Missed g Wrong Peak
PFNA 2 463.0/219.0 Da Baseline Missed Wrong Peak
KQ1000532-06
; :j
J\
IJb h .
PFOS 2 499.0/99.0 Da Baseline Missed a Wrong Peak
GI
::i..........
PFDa "2 513.0/269.0 Da c Baseline Missed Wrong Peak
/4
PFUnA2 563.0/269.0 Da l Baseline g Missed d Wrong Peak
PFDS 2 599.0/99.0 Da Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da c Baseline Missed c Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline Missed c Wrong Peak
PFTeA2 713.0/169.0 Da Baseline c Missed c Wrong Peak
Before After
Page 133 of 242 206
S e rv ic e s '
p. 71 Created with Analyst Reporter
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Project Data File Result Table Instrum ent Name
Ewan's ProjectsVPFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name A cq u isitio n Date Acquisition Method Injection Vial
KQ1000532-06 ( X A S 2/9/2010 9:57:46 AM PFCkinetexShort.dam 61
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
130-PFHxA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHxA
PFHpA 1
13C-PFHxA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
S 13C-PFDA
13C-PFDA
IS Area 4715000 5375000 3039500 5375000 5375000 3039500 4200600 4200600
Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.30 (6.32) 6.30 (6.31) 7.64 (7.65)
Analyte Area 9724500 10288000 18277000 10462000 3819000 17248000 3039500 4200600
Calc. Cone (ng/mL) 19.40 20.00 18.90 19.30 18.40 19.10 0.82 1.00
Modified? No No No No No No No No
$
cn ~ z ( l
Page 129 o f 245 207
S e rv ic e s '
Created with A n a ly it Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
I Analyte Peak Name
PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT) 1.26(1.27) 3.59(3.60) 4.25 (4.26) 5.29 (5.31) 6.21 (6.22) 6.30 (6.32) 1.26 (1.26) 5.29(5.30)
Area 9724500 10288000 7504800 1023400 11011000 7117900 4715000 5375000
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.41 (0.40)
YES
No 0.10(0.10)
YES
No 2.88(3.03)
YES
No 0.41 (0.40)
YES
No 0.48(13.55)
No
No 0.51 (13.06)
No
Page 130 o f 245 208
Quantitative Peak Review
p. 73 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000532-06
Before After
Page 131 o f 245 209
la
TIon Ratio Confirmation T.26
p. 74 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000532-06
4.
PFBA2 213.0/169.0 Da Baseline c Missed Wrong Peak
--24-
PFPeA2 263.0/219.0 Da o Baseline Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
:os M
PFHpA2 363.0/319.0 Da Baseline Missed c Wrong Peak
PFHxS2 399.0/99.0 Da Baseline n Missed o Wrong Peak
SJ3C-PFDA 515.0/470.0 Da Baseline Missed d Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
PFHxA 2 313.0/119.0 Da Baseline Missed d Wrong Peak
tenwisMi)te
N/A Baseline Missed
c Wrong Peak
Page 132 of 245 210
Analyte Name: Internai Standard:
eri/i s"
PFOA 1 13C-PFOA
p. 75 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Data File A c q u is itio n Date A cquisition Method Project
02081 OtACalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:________y = 0.0486 x + -0.00166 (r = 0.9989)'
Exp Cone (ng/mL)
# o f Values
Mean Calc Cone (ng/mL)
0.1 1 0.109 0.5 1 0.444
1 1 1.070 5 1 4.884 10 1 10.218 20 1 18.401 50 1 51.474
% Accuracy
109 89 107 98 102 92 103
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
-'L" 7 /Q t (ti
Page 1 of 242 211
f \ C o lu m b ia A n a ly tic a l S ervices''
p. 76 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFNA 1 13C-PFNA
Data File A cqu isitio n Date Acquisition Method Project
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's ProjectsVPFCs
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
y = 0.115 x + -0.00273 (r = Q.-9981)
# of Values
Mean Calc Cone (ng/mL)
1 0.109 1 0.467 1 0.975 1 4.961 1 10.803 1 17.941 1 51.343
% Accuracy
109 93 98 99 108 90
j103
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
Page 2 of 242 212
Columbia
A n a ly tic a l S ervices1
p. 77 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFOS 1 13C-PFOS
Data File A c q u is itio n Date A cquisition Method Project
02081 ObVCalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table A lgorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:________y = 0.107 x + -0.00155 (r= 0.9990) ^
Exp Cone (ng/ml_)
# of Values
Mean Calc Cone (ng/mL)
0.1 1 0.089 0.5 1 0.488
1 1 1.059 5 1 5.162 10 1 10.967 20 1 18.843 50 1 49.992
% Accuracy
89 98 106 103 110 94 10p 7
STD
NaN NaN
NaN NaN NaN NaN NaN
%CV
NaN
NaN NaN NaN NaN NaN NaN
!^ ;
t l U i iC
Page 3 of 242 213
p. 78
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFDA 1 13C-PFDA
Data File A cqu isitio n Date Acquisition Method Project
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20
_________ ___________
y = 0.106 x + -0.00306 (r = 0.9994) /
# of Values
Mean Calc Cone (ng/mL)
1 0.108 1 0.477 1 1.023 1 4.698 1 10.406 1 18.965 1 50.923
% Accuracy
108 95 102 94 104 95 102
STD
NaN NaN NaN NaN NaN NaN NaN
%cv
NaN NaN NaN NaN NaN NaN NaN
-<r. r.
Page 4 of 242 214
Cm um m sk
p. 79 Created with Analyst Reporter Piinted: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFUnA 1 13C-PFUnA
Data File A c q u is itio n Date A cquisition Method Project
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
/ Regression Equation:________y = 0.943 x + 0.0207 (r = 0.9983) `
Exp Cone (ng/mL)
# of Values
Mean Calc Cone (ng/mL)
% Accuracy
STD
%CV
0.1 1 0.092
92 NaN NaN
0.5 1 0.411
82 NaN NaN
1
1
1.199
120 NaN NaN
5 1 5.187 104 NaN NaN
10
1
10.978
110 NaN NaN
20 1 18.446 92 NaN NaN
50 1 50.287 101 NaN NaN
/
*
p / X<r r i /u
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f i C o lu m b ia A n a ly tic a l S ervices'
p. 80
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFDS 1 13C-PFOS
Data File A cqu isitio n Date Acquisition Method Project
Regression Equation:
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
02081 OfcACalLong.wiff
Result Table
02081 OSamplesLong.rdb
2/8/2010 6:00:11 PM
Algorithm Used
IntelliQuan
PFCkinetexLong.dam
Instrum ent Name API 5000
Ewan's Projects\PFCs
/
y = -0.000453 xA2 + 0.118 x + -0.00243 (r = O ^ S y )7
# of Values
Mean Calc Cone (ng/mL)
% Accuracy
STD
%CV
1 0.094
94 NaN NaN
1 0.490
98 NaN NaN
1 1.041 104 NaN NaN
1 5.064 101 NaN NaN
1 10.924 109 NaN NaN
1 18.516
93 NaN NaN
1
50.535
101
NaN
NaN
ii
Page 6 of 242 216
u a tu m i& a
Analytical Services'
p. 81 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFDoA 1 13C-PFDoA
Data File A c q u isitio n Date A cquisition Method P ro je c t
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
02081 ObVCalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's ProjectsVPFCs
Result Table A lgorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
/
# of Values
1 1 1 1 1 1 1
Mean Calc Cone (ng/mL)
0.082 0.518 0.985 5.302 11.377 19.829 48.506
% Accuracy
82 104 99 106 114 99 97
T
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
4
-Ly.
, iU i< c
Page 7 of 242 217
/ ? C o lu m b ia
*J Arelytkal Services''
p. 82 Created with Analyst Reporter Printed: 14/02/2010 5:41:00 PM
Analyte Name: Internal Standard:
PFTrA 1 13C-PFDoA
Data File A cqu isitio n Date Acquisition Method Project
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table A lgorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:________y = 0.352 x + 0.013 (r = 0,9 9 67 /'
Exp Cone (ng/mL)
# of Values
Mean Calc Cone (ng/mL)
0.1 1 0.062 0.5 1 0.567
1 1 1.018 5 1 5.785 10 1 11.661 20 1 18.568 50 1 48.939
% Accuracy
62 113 102 116 117 93 98
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
n ! ( jL&
Page 8 of 242 218
Columbia nrelytkal Services'
p. 83 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFTeA 1 13C-PFDoA
Data File A cq u isitio n Date A cquisition Method Project
02081 OtACalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong .dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:________y = -0.000306 x A2 + 0.27 x + -0.001 (r = 0.9948)
Exp Cone (ng/mL)
# of Values
Mean Calc Cone (ng/mL)
% Accuracy
0.1 1 0.080
80
0.5 1 0.587 117 1 1 0.928 93 5 1 5.191 104
10
1
11.943
119
20 1 50 _______1_____
17.075 50.817
85
102 /
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
.s '
A r iIM'L-
Page 9 of 242 219
'I/Lj CAonalulymtibciaal Services"
p. 84 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrum ent Name
Ewan's ProjectsVPFCs 02081 Ob\CalLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name A cqu isitio n Date Acquisition Method Injection Vial
IB 2/8/2010 6:00:11 PM PFCkinetexLong.dam 1
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA1
13C-PFD0A
PFTrA 1
13C-PFD0A
PFTeA1
13C-PFD0A
IS Area 305 253 326 97 33 326 139 139 139
Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.36 (2.53) 0.00(3.34) 4.16 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74)
Analyte Area 0 0
955 0
2031 0 0 0 0
Calc. Cone (ng/mL) N/A N/A 27.500 N/A 65.500 N/A N/A N/A N/A
Modified? No No No No No No No No No
'T '
S': \ i
k l*
Page 10 of 242 220
J Analytical Services'
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp R T ) 0.00(1.36) 1.25 (1.36) 2.25 (2.44) 2.48 (2.44) 2.29 (2.53) 2.77 (2.53) 0 .0 0 (3.34) 3.34 (3.34) 0.00(4.06) 4.17 (4.06) 4.06(4.10) 4.69(4.64) 4.85 (4.64) 5.14 (5.13) 5.59(5.56)
Area 0
305 63 253 255 326 0 97 0 33 365 635 139 376 236
p. 85 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Modified?
Calc (Exp) MRM Ratio
No 0.00 (2.38)
Ratio Confirms ID
No
No 0.00 (50.17)
No
No 0.00(0.28)
No
No 0.00 (19.17)
No
No 0.27 (0.32)
YES
No 0.34 (22.53)
No
No 0.00 (0.15)
No
No 0.00 (22.10)
No
No 0.00 (0.22)
No
No 0.02(1.86)
No
No 0.00(0.32)
No
No 0.00(0.29)
No
No 0.00 (3.08)
No
No 0.00 (0.32)
No
No 0.00(0.36)
No
Page 11 of 242 221
# \ WtuW^fci
c L 3 Analytical Services
Created with Analyst' Reporter Printed: 14/02/2010 6:41:00 PM
Quantitative Peak Review
1.25
I'i[ [i|Jli !; 1
IB
2.48
PFOA 1 413.0/169.0 Da l Baseline d Missed r Wronn Peak
13C-PFOA 417.0/372.0 Da c Baseline Missed ___n Wrnnn Peak___
.36
PFOS 1 499.0/80.0 Da Baseline Missed
p Wrnnn Ppak
4.16 . .y. o H i'1,'
PFUnA 1 563.0/519.0 Da Baseline q Missed p Wronn Peak
"*/r <\-r
2.77
13C-PFOS 503.0/80.0 Da Baseline Missed r Wrnnn Peak
I I: 4.17
M .......W-
13C-PFUnA 565.0/270.0 Da Baseline c Missed n Wrnnn Peak
PFNA 1 463.0/419.0 Da d Baseline Missed p Wrnnn Peak
PFDA 1 513.0/469.0 Da Baseline c Missed p Wrnnn Peak
13C-PFNA 468.0/423.0 Da c Baseline c Missed n Wrnnn Peak
3.34
i'll
13C-PFDA 515.0/470.0 Da Baseline Missed p Wrnnn Peak
\ "W i
1!.
PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak
2.77
13C-PFOS 503.0/80.0 Da a Baseline d Missed n Wrnnn Peak
> Wi. i!1
4.85,ii^iyh a,
`""hid ,F ii iilU i'i A i
I ij j j
* !! ( !
|1 4.85 j .
uili
PFDoA 1 613.0/569.0 Da c Baseline Missed p Wrnnn Peak
?%!\: ' ,iiifh1:'"AW; `i
'
'
:i;> 'i
r*1'pa|ryi!
13C-PFDOA 615.0/169.0 Da Baseline Missed r, Wrnnn Peak
rI jli P
PFTrA 1 663.0/619.0 Da Baseline Missed i - Wrnnn Paak
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Ppak
PFTeA 1 713.0/669.0 Da d Baseline Missed ___r Wrnnn Peak___
13C-PFDOA 615.0/169.0 Da Baseline Missed
n Wrnnn Peak
Before After
Page 12 of 242 222
rM .<***k>
CTM J Analytical Servkss
p. 87 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
ion Ratio Confirmation
:" i i
PFOA2 413.0/369.0 Da c Baseline Missed d Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed a Wrong Peak
I "1
IB
PFOS 2 499.0/99.0 Da c Baseline Missed 1 c Wrong Peak
i
, j . ............................. V
.'
PFDA2 513.0/269.0 Da I Baseline o Missed | d Wrong Peak
li fnj
M
i
i
PFUnA2 563.0/269.0 Da o Baseline c Missed c Wrong Peak
PFDS2 599.0/99.0 Da Baseline Missed o Wrong Peak
PFDoA2 613.0/169.0 Da a Baseline c Missed c Wrong Peak
PFTrA2 663.0/169.0 Da Baseline Missed Wrong Peak
l^
M
0i
4 .'--rV"-". a r c a v c
PFTeA2 713.0/169.0 Da c Baseline q Missed d Wrong Peak
Before After
Page 13 of 242 223
Columbia Analytical
Created with Analy^'F^eporter Printed: 14/02/2010 6:41:00 PM
P ro je c t D ata File A cquisition M ethod
Ewan's Projects\PFCs 02081 Ob\CalLong.wiff PFCkinetexLong.dam
Sam ple Nam e A cquisition Date A cquisition M ethod S a m p le ID Sam ple Com m ent
PFC 0.10ppb 2/8/2010 6:22:50 PM PFCkinetexLong.dam LCMS2-85A
Results Summary
Analyte Name
Analyte RT (Expected)
PFOA 1
1 .3 6 (1 .3 6 )
IS Name
1 3 C -P F O A
PFNA 1
2.31 (2.42)
1 3 C -P F N A
PFOS 1
2.35 (2.53)
1 3C -P F O S
PFDA 1
3 .3 2 (3 .3 4 )
1 3 C -P F D A
PFUnA 1
4.10(4.06) 13C-PFUnA
PFDS 1
4 .1 2 (4 .1 0 )
1 3 C -P F O S
PFDoA 1
4.76 (4.63) 13C-PFDO A
PFTrA 1
5.29 (5.14) 13C-PFDO A
PFTeA 1
5.74 (5.74) 13C-PFDO A
IS Area
2771900 2300200 6845000 2198200 162500 6845000 239910 239910 239910
R esult Table A lgorithm Used Instrum ent Name
Injection Vial Injection Volum e Sam ple Type Dilution Factor W eight to Volum e
02081 OSamplesLong.rdb intelliQuan API 5000
2 5 Standard 1.00 0.00
Analyte Area
10143 22540 54526 18255 17391 59395 15176 8315 4924
Exp Cone (ng/mL)
0.100 0.100 0.100 0.100 0.100 0.100 0.100 0.100 0.100
Calc. Cone (ng/mL)
0.109
Accuracy
(%)
109
0.109
109
0.089
89
0.108
108
0.092
92
0.094
94
0.082
82
0.062
62
0.080
80
Mod? No No No No No No No No No
i ,,w fJ
'U
(tO ( <4
Page 14 of 242 224
wiwx * ,3 Analytical Services
IRC Summary
Analyte Peak Name
PFOA 2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S_13C -PFO S PFDA 2 S_13C -PFDA PFUnA2 S_13C -PFUnA PFDS 2 PFDoA2 S_13C -PFDoA PFTrA 2 PFTeA2
RT (Expected)
1.33 (1.36) 1.35 (1.36) 2.32 (2.44) 2.28 (2.44) 2.33 (2.53) 2.32 (2.53) 3.33 (3.34) 3.32 (3.34) 4.10 (4.06) 4 .1 0 (4 .0 6 ) 4.12 (4.10) 4.75 (4.64) 4.76 (4.64) 5.29 (5.13) 5 .7 5 (5 .5 6 )
p. 89 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Area
19840 2771900
3319 2300200
16682 6845000
2768 2198200
2974 162500 17921
2899 239910
2346 1505
Modified?
No No No No No No No No No No No No No No No
Calculated Ion Ratio (Expected Value)
1 .9 6 2 7 3 .2 8
0 .1 5 1 0 2 .0 5
0.31 1 2 5 .5 4
0 .1 5 1 2 0 .4 2
0 .1 7 9 .3 4 0 .3 0 0 .1 9 15.81 0.28 0.31
Page 15 of 242 225
C o lu m b ia c L J A n a ly tic a l S ervices"
iQuantitative Peak Review
1.35
PFOA 1
413.0/169.0 Da Baseline Missed
n Wrnnn Peak
2.35
13C-PFOA 417.0/372.0 Da Baseline Missed n Wrnnn Peak
L
IPFOS 1
499.0/80.0 Da u Baseline Missed
i Wrnnn Peak
PFUnA 1 563.0/519.0 Da c Baseline c Missed ! Wrnnn Peak
1 13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak
I
p. 90 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
PFC O.IQppb
iPFNA 1
463.0/419.0 Da Baseline Missed
n Wrnnn Peak
13C-PFNA 468.0/423.0 Da o Baseline c Missed
Wrnnn Peak
PFDA 1 513.0/469.0 Da Baseline Missed
n Wrnnn Peak
4.12
13C-PFDA 515.0/470.0 Da
Baseline a Missed n Wrnnn Peak 2.32
PFDS 1
599.0/80.0 Da
Baseline Missed __ n Wrnnn Peak
5.29
13C-PFOS 503.0/80.0 Da
u Baseline Missed p Wrnnn Peak 4,76
PFDoA 1 613.0/569.0 Da
c Baseline Missed r Wrnnn Peak 5.74
13C-PFD0A 615.0/169.0 Da Baseline Missed p Wrnnn Peak
PFTrA 1 663.0/619.0 Da Baseline Missed ___n.Wrnnn Peak___
13C-PFDoA 615.0/169.0 Da Baseline d Missed ___ r Wrnnn Peak___
PFTeA 1 713.0/669.0 Da c. Baseline Missed i Wrnnn Peak
13C-PFDoA 615.0/169.0 Da Baseline Missed ___n Wrnnn Peak___
Before After
226
Page 16 of 242
p. 91 Created with Analyst Reporter
nSs<5! Printed: 14/02/2010 6:41:00 PM
Ion Ratio Confirmation
- . :"
1.33
i .'
!\ i.2t - .
PFOA2 413.0/369.0 Da l Baseline c. Missed c Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak
PFC 0.10ppb
k
.L
PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak
.3 A
PFDA2 513.0/269.0 Da Baseline c Missed l. Wrong Peak
1 ..:
4.1 D
PFUnA 2 563.0/269.0 Da Baseline Missed c Wrong Peak
PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da c Baseline Missed o Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak
5 ............ ' . rA/U^Ar' jf w V W
PFTe4 2 713.0/169 .0 Da c Baseline l Missed u Wrong Peak
Before After
Page 17 of 242 227
/ ? C o lu m b ia
Analytical Servicer
p. 92 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
P ro je c t D ata File A cquisition Method
Ewan's Projects\PFCs 020810b\CalLong.wiff PFCkinetexLong.dam
S am ple Nam e A cquisition Date A cquisition M ethod S a m p le ID Sam ple C om m ent
PFC 0.5ppb 2/8/2010 6:30:24 PM PFCkinetexLong.dam LCMS2-85B
Results Summary
Analyte Name
Analyte RT (Expected)
PFOA 1
1 .3 7 (1 .3 6 )
PFNA 1
2.34 (2.42)
PFOS 1
2.36 (2.53)
PFDA 1
3.34 (3.34)
PFUnA 1
4 .1 2 (4 .0 6 )
PFDS 1
4 .1 3 (4 .1 0 )
PFDoA 1
4 .7 7 (4 .6 3 )
PFTrA 1
5.29 (5.14)
PFTeA 1
5.74 (5.74)
IS Name 1 3 C -P F O A 1 3C -P F N A 1 3C -P F O S 1 3 C -P F D A 1 3 C -P F U n A 1 3 C -P F O S 1 3C -P F D O A 13C -P F D O A 13C -P F D O A
IS Area 2869800 2221600 6831100 2150700 185430 6831100 197790 197790 197790
R esult Table A lgorithm Used Instrum ent Name
Injection Vial Injection Volum e Sam ple Type D ilution Factor W eight to Volum e
02081 OSamplesLong.rdb IntelliQuan API 5000
3 5 Standard 1.00 0.00
Analyte Area 57106
112750 344960 101810 75738 378480 59384 42023 31152
Exp Cone (ng/m L)
0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500
Calc. Cone (ng/m L)
0.444
Accuracy (%)
89
0.467
93
0 .4 8 8
98
0.477
95
0.411
82
0.490
98
0.518
104
0.567
113
0.587
117
7
Mod? No No No No No No No No No
*
iJl
Page 18 of 242 228
r\y>
Analytical
IRC Summary
Analyte Peak Name
PFOA 2 S_13C_PFOA PFNA 2 S _13C P FN A PFOS 2 S_13C -PFO S PFDA 2 S_13C -PFDA PFUnA 2 S_13C -PFUnA PFDS 2 PFDoA2 S_13C -PFDoA PFTrA 2 PFTeA2
p. 93 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
RT (Expected)
1.37 (1.36) 1.36 (1.36) 2.32 (2.44) 2.33 (2.44) 2 .3 5 (2 .5 3 ) 2 .3 5 (2 .5 3 ) 3.36 (3.34) 3.33 (3.34) 4.13 (4.06) 4.13 (4.06) 4.13 (4.10) 4.76 (4.64) 4.76 (4.64) 5.30 (5.13) 5 .7 4 (5 .5 6 )
Area
148080 2869800
32633 2221600 110050 6831100
15344 2150700
18267 185430 117570 19133 197790 12533 12051
Modified?
No No No No No No No No No No No No No No No
Calculated Ion Ratio (Expected Value)
2.59 5 0 .2 5 0 .2 9 1 9 .7 0 0 .3 2 1 9 .8 0 0 .1 5 2 1 .1 2 0 .2 4 2 .4 5 0.31 0 .3 2 3 .3 3 0 .3 0 0 .3 9
Page 19 of 242 229
/ ? Columbia A n a ly tic a l Services"
Quantitative Peak Review
1.36
p. 94 Created with Analyst Reporter
Printed: 14/02/2010 6:41.00 PM
PFC 0.5ppb
2.33
PFOA 1 413.0/169.0 Da c Baseline c Missed i Wrnnn Peak
13C-PFOA 417.0/372.0 Da c Baseline Missed
r Wrnnn Peak
2.35
L
PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak
13C-PFNA 468.0/423.0 Da g Baseline Missed
n Wrnnn Peak ______ 3 ^3 ________
PFOS 1 499.0/80.0 Da c Baseline Missed n Wrnnn Peak
4.12
1
13C-PFOS 503.0/80.0 Da c Baseline Missed r Wrnnn Peak
4.13
1
PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak
13C-PFDA 515.0/470.0 Da c Baseline c Missed
Wrnnn Peak
PFUnA 1 563.0/519.0 Da Baseline Missed r Wrnnn Peak__
4.77
13C-PFUnA 565.0/270.0 Da Baseline Missed ___n Wrnnn Peak__ _
PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFOS 503.0/80.0 Da Baseline Missed
n Wrnnn Peak
PFDoA 1
613.0/569.0 Da g Baseline Missed
r Wrnnn Peak 5.74
13C-PFDOA 615.0/169.0 Da
o Baseline Missed r Wrnnn Peak
PFTrA 1 663.0/619.0 Da Baseline d Missed ___r W rnnn Peak___
13C-PFDOA 615.0/169.0 Da Baseline c Missed ___r Wrnnn Peak
PFTeA 1 713.0/669.0 Da Baseline c Missed i Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed i Wrnnn Peak___
Before After
230
Page 20 of 242
,f i ro lu m b ia
< 3 Arektical Services"
Created with A nalyst^eporter Printed: 14/02/2010 6:41:00 PM
Ion Ratio Confirmation
:::;----------1- f - -----------
A
i ......... 4 - . . . . . .
PFOA2 413.0/369.0 Da n Baseline c Missed l Wrong Peak
PFNA2 463.0/219.0 Da Baseline n Missed u Wrong Peak
PFC 0.5ppb Aj
1
i-
PFOS2 499.0/99.0 Da Baseline Missed u Wrong Peak
:____ --
PFDA2 513.0/269.0 Da Baseline c Missed c Wrong Peak
:rJz
PFUnA 2 563.0/269.0 Da Baseline Missed d Wrong Peak
PFDS 2 599.0/99.0 Da o Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da d Baseline Missed c Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline Missed [_ Wrong Peak
Before After
Page 21 of 242 231
/ ? C o lu m b ia c L A n a ly tic a l S arvioe s"
p. 96 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
P ro je c t D ata File A cquisition M ethod
Ewan's Projects\PFCs 02081 Ob\CalLong.wiff PFCkinetexLong.dam
Sam ple Nam e A cquisition Date A cquisition M ethod S a m p le ID Sam ple C om m ent
PFC 1.0ppb 2/8/2010 6:37:56 PM PFCkinetexLong.dam LCMS2-85C
Results Summary
Analyte Name
Analyte RT (Expected)
PFOA1
1.33 (1.36)
IS Name 1 3 C -P F O A
IS Area 2783400
PFNA 1
2.31 (2.42)
1 3 C -P F N A
2203300
PFOS 1
2.34 (2.53)
13C -P F O S
6650300
PFDA 1
3 .3 2 (3 .3 4 )
1 3 C -P F D A
2119100
PFUnA 1
4.12(4.06) 13C-PFUnA
155180
PFDS 1
4 .1 2 (4 .1 0 )
1 3C -P F O S
6650300
PFDoA 1
4.75 (4.63) 13C -P F D o A
234070
PFTrA 1
5.29 (5.14) 13C -P FD oA
234070
PFTeA 1
5.74 (5.74) 13C-PFDO A
234070
R esult Table A lgorithm Used Instrum ent Nam e
Injection Vial Injection Volum e Sam ple Type D ilution Factor W eight to Volum e
02081 OSamplesLong.rdb IntelliQuan API 5000
4 5 Standard 1.00 0.00
Analyte Area
140010 240150 740480 222700 178520 800030 129680 86772 58420
Exp Cone (ng/m L)
1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000
Calc. Cone (ng/m L)
1.070
Accuracy (%)
107
0.975
98
1.060
106
1.020
102
1.200
120
1.040
104
0.985
99
1.020
102
0.928
93
/
Mod? No No No No No No No No No
\U
-V
Page 22 of 242
232
IL 3"2 Ar Anlaiilmytikcbal Sen/kcs"
IRC Summary
Analyte Peak Name
PFOA 2 S_13C_PFOA PFNA 2 S_13C_PFNA PFOS 2 S 13C -P F O S PFDA 2 S_13C -PFDA PFUnA2 S_13C -PFUnA PFDS 2 PFDoA 2 S_13C -PFDoA PFTrA 2 PFTeA2
RT (Expected)
1.35 (1.36) 1.31 (1.36) 2.28 (2.44) 2.27 (2.44) 2.30 (2.53) 2.31 (2.53) 3.31 (3.34) 3 .3 2 (3 .3 4 ) 4.13 (4.06) 4.11 (4.06) 4.12 (4.10) 4.75 (4.64) 4.75 (4.64) 5.31 (5.13) 5.75 (5.56)
Created with Analyst R e a d e r Printed: 14/02/2010 6:41:00 PM
Area
331790 2783400
72566 2203300 228880 6650300
29896 2119100
41151 155180 253850 42524 234070 29476 19065
Modified?
No No No No No No No No No No No No No No No
Calculated Ion Ratio (Expected Value)
2 .3 7 1 9 .8 8 0 .3 0 9 .1 7 0.31 8 .9 8 0 .1 3 9.52 0 .2 3 0 .8 7 0 .3 2 0 .3 3 1.80 0 .3 4 0 .3 3
Page 23 of 242 233
/ C o lu m b ia
Analytical Services''
Quantitative Peak Review
p. 98 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
PFC 1.0ppb
2.27
1
PFOA 1 413.0/169.0 Da c Baseline c Missed r Wrnnn Peak
13C-PFOA 417.0/372.0 Da Baseline Missed
J~: WrQn,O..P.flak
PFNA 1 463.0/419.0 Da
Baseline Missed ri Wrnnn Peak
13C-PFNA 468.0/423.0 Da Baseline o Missed __ n Wrnnn Peak
PFOS 1 499.0/80.0 Da c Baseline Missed 11 Wrnnn Peak
PFUnA 1 563.0/519.0 Da a Baseline c Missed r Wrnnn Peak
4.75
L 13C-PFOS 503.0/80.0 Da d Baseline Missed r Wrnnn Peak
13C-PFUnA 565.0/270.0 Da Baseline c Missed p Wrnnn Peak
PFDA 1 513.0/469.0 Da Baseline u Missed n Wrnnn Peak
PFDS 1 599.0/80.0 Da c Baseline a Missed
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak i 21 1
1
13C-PFOS 503.0/80.0 Da c Baseline Missed n Wrnnn Peak
PFDoA 1 613.0/569.0 Da l Baseline Missed r Wrnnn Peak
5.74
13C-PFDOA 615.0/169.0 Da c, Baseline Missed n Wrnnn Peak
PFTrA 1 663.0/619.0 Da
Baseline Missed n Wrnnn Peak___
13C-PFDOA 615.0/169.0 Da Baseline c Missed ___n Wrnnn Peak___
PFTeA 1
713.0/669.0 Da
l Baseline c Missed
r Wrnnn Peak
13C-PFDOA 615.0/169.0 Da c Baseline Missed n Wrnnn Peak
Before After
234
Page 24 of 242
/ ? Columbia 3 Analytical Service??
Ion Ratio Confirmation
1.35
p. 99 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
PFC 1.0ppb
PFOA2 413.0/369.0 Da Baseline c Missed l Wrong Peak
PFNA 2 463.0/219.0 Da j Baseline Missed u Wrong Peak
PFOS2 499.0/99.0 Da c Baseline Missed c Wrong Peak
PFDA2
513.0/269.0 Da
Baseline Missed lj Wrong Peak
PFUnA 2
563.0/269.0 Da
c Baseline u Missed l Wrong Peak
PFDS2 599.0/99.0 Da g Baseline Missed d Wrong Peak
PFDoA2 613.0/169.0 Da c Baseline Missed Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline c Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline c Missed c Wrong Peak
Before After
Page 25 of 242 235
A n a ly tic a l Services'
p. 1 0 0 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Acquisition Method
Ewan's Projects\PFCs 020810b\CalLong.wiff PFCkinetexLong.dam
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Sample Name A cqu isitio n Date Acquisition Method Sample ID Sample Comment
PFC 5.0ppb 2/8/2010 6:45:29 PM PFCkinetexLong.dam LCMS2-85D
Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume
5 5 Standard 1.00 0.00
Results Summary
Analyte Name
Analyte RT (Expected)
PFOA 1
1 .3 9 (1 .3 6 )
PFNA 1
2.30 (2.42)
PFOS 1
2.36 (2.53)
PFDA 1
3 .3 4 (3 .3 4 )
PFUnA 1
4 .1 2 (4 .0 6 )
PFDS 1
4 .1 3 (4 .1 0 )
PFDoA 1
4.76 (4.63)
PFTrA 1
5.29 (5.14)
PFTeA 1
5.76 (5.74)
IS Name 1 3C -P F O A 1 3C -P F N A 13C -P F O S 1 3 C -P F D A 1 3 C -P F U n A 1 3C -P F O S 1 3C -P F D O A 13C -P F D O A 13C -P F D O A
IS Area 2766100 2154600 6761100 2111500 172740 6761100 199680 199680 199680
Analyte Area
651680 1218400 3709300 1041800 848100 3957300 578950 408680 278340
Exp Cone (ng/m L)
5.000 5.000 5.000 5.000 5.000 5.000 5.000 5.000 5.000
Calc. Cone (ng/m L)
4.880
Accuracy (%) 98
4.960
99
5.160
103
4.700
94
5 .1 9 0
104
5.060
101
5.300
106
5 .7 8 0
116
5.190
104
/
Mod? No No No No No No No No No
\ fU I !0
Page 26 of 242 236
