Document 6BBLjvwEMk41jN3YxaVoyKoZm

FOR DUPONT USE ONLY AR226-2791 Approximate Lethal Concentration of Haskell Laboratory Report No. 276-85 MR No Haskeli E. I. du Pont de Nemours and Company Laboratory for Toxicology and Industrial P.O. Box 50. Elkton Raod Newark, Delaware 19714 Medicine Date Issued: Ju^y 9, 1985 C^^ Sanitized. Does not contain TSCA CB Approximate Lethal Concentration of lin Pats SUMMARY Groups periods to study, the considered owff\^ of 6 male Cri :CD(SD)BRj,atswere exposed for single 4-hour vapor concentrations ai''. Under the conditions of approximate lethal concentration was 160 mg/L. This material to have very low toxicity by inhalation. this is 2 - - Coiaspa'.^^ Sanitized. Does not contain TSCA CBB HLR 276-85 Work by: ^ ^ ^.^^p Cfarence W. Hutt. Ill Technician 6 13-18- J^^. ^U^A T Robert T. Turner Technician ^i^i^ '^^A^(L^ Thomas y, Kegelman Technician ^ITI^ Supervised by: ^a^.^ A . IvL^-_____6/S"/ 3S' Laura A. Kinney Chemist Reviewed by: ^4^---- WB/ --------- p^Ke^ly ebloa-fst ^ 1^1^ Approved by: ^6^., PG^L^CX</ Nancy V. Chromey, Py?0. Study Director Section Supervisor, Acuts Investigations 7/^7^ Acknowledgements: Bruce A. Burgess also participated in the conduct of this study. DPK:TAK:smk:HLR9.6 - 3 - Conp.n,S,n,>,zed.Dono,c.n.ainTSC*CBl Haskell Laboratory Report No. 276-85 MR No.f|HT) Haske11 No- 14y808 Material Tested: Sponsor: Chemicals and Pigments Department E. I. du Pont de Nemours and Company Wilmington, Delaware Material Submitted By: Chemicals 8 Pigments Department Jackson Labo-atory Test Facility: E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine P.O. Box 50, Elkton Road Newark, Delaware 19714 Study Initiated-Completed: 3/22/83 - 4/11/83 'There are 7 pages in this report. Distribution: 4 - Company Sanitized. Doss not captain TSCA CBS INTRODUCTION HLR 276-85 The purpose of thisstudy was to determine a 4-hour approximate lethal concentralon (ALC) fotfffl^n rats. The ALC Is defined as the lowest atmospheric concentratTon tested that caused the death of one or more rats either on the day of exposure or within 14 days post exposure. Except as documented In the study records, this study was conducted according to the applicable Good Laboratory Practice Regulations. MATERIALS AND METHODS A. Animals Young adult male Cri:CD(SD)BR rats were obtained from Charles River Breeding Laboratories, Kingston, New York. Each rat was assigned a unique 6-digit Identification number which corresponded to h numbered card affixed to the cage. Rats were quarantined for one wee< prior to testing, and were weighed and observed twice during the quarantine period. During the test, rats were housed in pairs in 8" x 14" x 8" suspended, stainless steel, wire-mesh cages. The rat assigned the lower number in each cage was identified by a slash in the right ear. Rats' tails and cage cards were color-coded with water-insoluble markers so that individual rats could be identified after exposure. Except during exposure, Purina Certified Rodent Chow* *b002 and water were available ad ~ Ubitum. Exposure Protocol Groups of 6 rats, 8-9 weeks old and weighing between 231-322 grams wereexposed -hole-body for a single 4-hour period to a vapor atmosphere ofwIHnin air. Rats were weighed prior to exposure and were observed for^nnical signs of toxicity during exposure. Surviving rats were weighed and observed daily for 14 days post exposure, weekends excluded, except when deemed necessary by the rats' condition. C. Test Material Physical Form; Composition: Company Sanitized. Do.s not co.tain TSCA CBl Synonyms: HLR 276-85 CAS Registry No.: Stability: The test material was assumed to be stable throughout the exposure phase of the study. 0. Atmosphere Generation Vapor atmospheres o^HlB<ere generated by pumping the liquid into a round bottom flask. The flaslTwas at ambient temperature for the first ALC exposure. In the remaining four exposures, the round bottom flask was heated to approximately 62-121C to attain higher concentrations. The resulting vapors were carried by houseline air to the 17 liter glass exposure chamber. The atmospheric concentration otj----^as determined at approximately 30-minute intervals by drawing sampresof the test atmosphere from the exposure chamber with a glass syringe. Samples were analyzed isothermally at 90C with a Hewlett-Packard 5790A gas chromatograph e*. .ipped with a flame ionization detector and a 6" X 1/4" 0.0. glass column packed with 10 % SE 30 on Supelcoport 80/100 mesh. Concentrations were determined by comparison with standard curves, which were.prepared daily. Gas standards were prepared by evaporating liquidflH^n calibrated glass bottles. w ' Chamber temperature was measured .."'th a mercury thermometer, and chamber oxygen ^as measured with a B-ioMarine Model 225 Oxygen Analyzer. F. Records Retention All raw data and the final report will be stored in the archives of Haskell Laboratory for Toxicology and Industrial Medicine, Newark, Delaware, or in the DuPont Hall of Records, Co., Wilniington, Delaware. E. I. du Pont de Nemours and Compa"y'sa^e-."-------"1""^"1 RESULTS HLR 276-85 A. Exposure Conditions and Associated Mortality Chamber temperatures ranged between 26-30C and chamber oxygen content was 211; Atmospheric concentration and mortality data are presented below. Atmospheric Concentration-; nfl^Mlnil Oiipciated Rat Mortality ----^ i^-------------------------------- Concentration (mg/L) 'leanS.D. 4.9 1.8 20 4.1 61 2.2 160 4.5 290 :J Range 0.17-5.7 9.7-23 56-63 150-160 280-300 Fractional Mortality ^ deaths/ w exposed 0/6 0/6 0/6 5/6 6/6 B. Clinical Observations During exposure, no clinical signs of toxicity were observed in rats exposed to 4.9, 20, or 61 mg/L. Rats exposed to 160 mg/L exhibited labored breathing and salivation. Rats exposed to 290 mg/L exhibited pale ears, labored breathing, staggering and sporadic movement. All deaths occurred during exposure. The sole survivng rat exposed to 160 mg/L exhibited slight weight loss (3,01) on the day after exposure, foil-owed by a normal weight gain throughout the recovery period. During the 14 day recovery period, some surviving rats exhibited Slight weight loss at 24 hours post exposure. No other abnormalities were observed. CONCLUSIONS Under the conditions of this test, the inhalation ALC ofUH^pn rats was 160 mg/L. This material is considered to have very low toxicity5y inhalation. 7 - Company Sanitized: Doe^ not contain TSCffiCBS