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BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-203. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumbers were not noted. The test sample isFC-203. Currentinformatioinndicatesitisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37-85% water,20% ethyleneglycol, 2.66 % Sultonefoamer,3% sodium octylsulfate0,.1% sodium laurylsulfate, and 0.05% tolyltfiazole. The followingisan abbreviated summary of muldple biodegradation studies on FC-203. These studies apply to a mixture with incompletely characterized concentrations of impurities.Date does not accurately reftct the degradation potendat of the fluorochemical component of the testsample. METHOD: Method: Va@ous methods, most not noted. Type: BOD and Dichromate RefluxCOD GLP: No Year completed: 1973 - 1980 Contact time: 20 days lnoculum: Seed from varioussources. RESULTS Chemical Oxygen Demand (COD) range: 420,000 - 1,070,000mg/kg BiochemicalOxygen Demand (BOD) 5-Day: 1O-Day: 12-Day: 15-Day: 18-Day: 20-Day: range: 206,250 - 560,000 mg/kg 450,000- 646,875 rrtpg 660,000 - 830,000 mg/kg 426,312 - 604,687 mg/kg 660,000 mg/kg 426,562 - 1,100,000nig/kg Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS No reliablceonclusionscan be derivedfrom these studies.The indicatioins thatthe majorityofthe productcomponents are readilybiodegradable. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilityK:limischranking= 3. Study lacksdescriptioannd recordsofthe method followed.The inoculum isnot properlycharacterizedT.he testing was conducted on a mixturewithchemicalshavingvaryingdegrees of biodegradabilitlye,adingto potentiamlisinterpretatiofnthe results.The sample puritywas not propedy characterizedand the studylacksanalytical confirmatioonfthe amount offluorochemicalproportioninthe solution. REFERENCES The studieswere conducted by the 3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request numbers 1940 and 5360S in 1973. OTHER Last changed: 6/28/00 Porm 10620-5.PWO cc: LAS REQUEST BOOK - 42.5W Ref,@INVI@R. AS ES,@. IN Q., # L4rAw(t4 Ck^ PRODUCT'. i-C -e-oz PRODUCT REQUEST DIVIS15N- ECOLOGICAL ASSESSMENT FOR LABORATORY WORK LAB REQUEST No. 3M. REQUESTER Name: nr (-I 4"r, Phone No. 509 Reque s-tdate: ProjectNo.: Prob. No. Code No.: Sample Date: IJevJ '@tt,@To Date Needed: T-oC- 4 SA P Description: #Vcdo 6aiolCoiO Sfcdy o*FC-zd3 Purpose of Tests: j., "esi-/f3 4r-C, Ael' (6-3'J f?,% t- - &L4,L ttg44 S gti'-iOiAl PHYSICAL & CHEMICAL PROPERTIES HeatofCombustion Cal/G (BTU/Lb.) Density -Ash Other LABORATORY Date Received SamQle- Analyst: w ft - of Date Completed Hours Spent: 8'71t, y -B-P 2 le"i.c.S-;,Orp PleaseCallRequester ItDelays Are Encountered. WATER POLLUTIBN ow- CIOD - )c COD - I )< BOD 5 )< BOD, Ultimate TOC 4r7C,wo Ito-) . - -Ge, bdm A.@,t Oxygen Uptake DehydrogenaseActivity(TTC) c@@ Other A/OTC-5.1 -roc- 4L /.IW,cco .00 yyl- m'7 0. 00 )r ofi?7-s-I '1600 FFrT., E 84sca A\ie&4F7be- BIOASSAY SpecifyOrganism and Method: PEI 4C- LEACHATE FROM LEACHING PH COD BOD5 BOO, U!timate TOC GC Bioa!;Say Other TEST OTHER Bioaccumulation Persistence DegradationProducts Ot 801).12. ioi@o. a, 920, 04!0 AAP A LrAe.&l-.4A 7'e,j&@: )Silo 9 oaa i OoD White - Return to Requesto Carorv-Environrnentsfii@- Offl. Fli.' Plink-EnvirorwnwnalErma. Lob 'Goldenrod nowm to asou@ter EnvironnwtaLlabwatory WO Work ShoK -Sompla Lab Rtq-NO AngoVit 000Run ft.# S?O- OWA ftwftd DayBOD Readinp Ame" Date pl*Dowiption lmdd ft"W OWIlm Fasew ii2iOQ at Swnpledilutetdo 2@,TO miwithD Iwater 10ilutiofnacto-r I ifundiluted) ft"le Gem d #I& %Wfbi 99 yea 23- 13.3 9^ 01) C-5c) i.o 2,o piam D& mal TO* DA16 tDi -IV 1, --2-,-00 4-Yo D@Pled"ow IB) 1411000 ).(#(p 49)6.OW Day DOD Note: 1. Conc. Concentration of umpi4 (mi/i BOO battles. 2. Sample Size mi or 9 @f sunple or dilutedumple added to SOID Battle. Comments 19756 PWO Envir,innw-ItLWabor&WY .130DWork ghw. sanipin abR*q@NO-6 @nsiva ---------- 4E)4.).rtA- DOWIPO" 0-5 some comai 123 #,fez 13.3 1#0 2,0 3m Fw Noo.-** f?O- S' DW oW ftosdbw A .49@0i0 pa LOT- I ofswwm dilutetdo mAwitthhDiwater (g)JlMUtiigatoi I If#tA@nWduiwiuuitde@d) TOW Dia. 0 W40 a& ow cov 7-Ad my 900 F- Not$: 1. Conc. Conantratioonfwnpwiw@ inBOD bottin. 2. SOMPieSin W or9 ofS@mpleordiluteUdMPIObddw tO000 Botdo' com"nu Form 19756 PWO EnwitOnnw*%W gt&Y BOD wo* shot -sompla -bRo%No.$ r4)4.)C4.4- Sc, oew*lw* L-oDdidkilim ud"DowdFo~m sews 23 C> 2#0 a&oil DOD Run No. .,IC)- DW BOD Roodko - WD R" LOT- rmwit0h1water (Diluftaicomn Iufrkdiiuted) Tod0.& 0 -OW -7Ad 'AN~ -20 3,-(p j Note: cone. carmntmdw of$owl* (adA BW bottlb:. SwnpleSize allofg otsunpleordilutesdunpisaddedtoBOO Botue. Conwnents Form 19766PWO -4 EnvironmiiLnatbaolrs" BW WorkShoot-Nmb. controobw,sta,dao- LobRequesNto. C" Se moo Run No. 3m Day SOD Readinp mom (All 7, etc?s .470 0 gsWW tmkodm -Wow mink kediwa$ hww DAL ;?I gi Da 04*"* IAI -Aal (D 4@o; el Cal Avmmp DA. Dopledon (Al A2) A"rop D.O. S"d Con#Ws 2 Ll 9, .30 lr" fall homm D& 2-n 9-,.40 $70 7. 7-@@. 0 70 0', 0.0. 3,@5D Gkmu Gkian* AcM SUN"D "ttm P". smqvw Sim W" oil ft*Mt 0.0. 02) PotuMuHmydropnPhduiauStwidudtoptimw) 8ottNloe. SwnplSeize hAtWD.O. oil Fi@ tDM. ID2) Teem 0.0. Dowd" (01 -D2) 7 o 2-0 rl-::Wt't-5le 0"4"6" DW Tosommm Dw am rig@. ow 0 C) lit N q-5 2-(. q,-5C, TotaDl.O. Ow"on (DI 021 DepladmDue To 0' ..coy Avwsp, SOD- Day Form 19755 PWO Envkonnwm LAbwo" V" ib"W" cz,@@ - 12.9 INM O&AM oo"lo 13 tail W@W NO& b"d DA16 S*W Controls Battle No. WED" 160- V- -CW BW R"dinp -c-, 19 9&'70 1a E. 0__ - I Awwop DA. 0""don Avwep DA. Z!d" 21 OiE] 0.0. copo all Glucose - Glutwnic Acid SUOdUd Battle No. son"" sin 3 0 Potassium Hydrogen Phduhft bottle No. Sampit Sin IDI) ID21 5-0 SUrAwil (OpdonW) . frAIW D.C. oil Present 0.0.* ID2) Toltd D.O. IDI -D2) Too- 4, i.!Yg -so@raoo" T. Totg D.G. Dopbbon 401-02) aspietim ow To Stwmiwd 1 lf77 IS- DOV FA_VOAv6wo0p 0 0W Boo Form1975P'W3O limikonim moo wolta" -okras, convokoNd owww* b RorjWNO. WA NO. 7-0 go- q@r-Ow KO NoodkW 00odw WOW VFWAMh 12X a-dip JAI o 6) Dopkfflw IXI X2) 13 sawcoovab 60"10 No. %0910 somplegi n . id0 bag" a& k4" DA16 all 7. 7 all 3,60 o. *mw D.O. o, as-%I 1#1 41 alucoo - Glutmk #Aid $UvWwd Boni*1". $"M" $in lewd 0 A.* hamt 0.0. 021 3 Pounium Hydmpn Phdataft Bonk No. sompis sin ttmmiwd (OPTIOW) &WOW DA. IDII Po@ t D.O. (D2) Tad OM. 044own IDI -D2$ Dwiodm a" TO sura" .3!r L3.oo S@-59 7, 15@ .0w @3(7+ 331fp @. 4 A Totw 0.0. D$Pbtim'Due O&V DWetion To Sondwd to, -D2) .71 Form 19755 PWO lo-I IPPV^ 'rc. zoo rc -zoo. -ZO@4, 0'37. 2 00 soot 000 14- AIS zo-bkis 24-bo4@s 450000, 4@5.000 340000 ewtooo 46,Oft 41s,ooo 540,ooo IbAvi 20-b^ft 2A.'&lo 2F24A-btAk,AYS 4!5,000 4@5,560 60@4poo 540,060S-b&is i7,bAys Lek% o44- AN @-04- I%%ZR Z4,OW 12-b4s IS-D^ys -37,1.500 12-tAll 12-b*,ys 19-bAls ZAOIOOT 4*W.000 "0= -uy C. 1'2-bAls %VAGOYO- WOj-- S-bk(c. '12bA @.o *b4A OOAC4 FORM'10620-PWO cc:LAB iEQU@ST BOOK - 42-SW File: re-pol Water and Wastewater REQUEST FOR LABORATORY WORK Lab Request No. TO: ENVIRONMENTAL ENGINEERING LABORATORY - BUILDING 53-6N Requestor Name: "gt4c-,D-A)-RequestDate 12-, ProjectNo@7-t2O7--lrProb No 0 -? Code Description: c::5 Sample Date: Date Needed: Hold Sample For -Days Est.Time: Hrs. Lab Personnel Date Rec'd.SamDle:. I I - -I a - I Analyst C- r I*k%A@ t -%-- y B P Date Completed: Hrs. Spent: Sample Description Total Hardness, mg/i as CaC03 Calcium Hardness, mgA as CaC03 7'P-"Alkalinity,Mg/i as C8C03 "M" Alkalinity,mg/l as CaC03 TH Sulfate,mg/l as S04 Nitrate,mg/i as N03 Chloride, mg/l as Cl Silica,Mg/i as Si02 Fluoride, mg/i as F Ortho Phosphate, mg/l as P Total Phosphate, mg/i as P Total Chromium, mg/i as Cr ffe-x.Chromium, mg/l as Cr Total Cyanide, mg/l as CN Ferrocyanide, mg/i as CN n, mg/i as Fe Manganese, mg/i as Mn Surfactant,mg/i as LAS Phenol, mg/i BOD, mg/i r COD, mg/l (ShortLLanguilute) Total Suspended Solids,mg/i Volatile Suspended Solids,mg/i Turbidity, mg/l Silica Total Solids, mg/l Total VolatileSolids, mg/i Conductivity, mhos Total Bacteria,No./100 mi 7-otalColiform, No./100 ml FecalColiform,No./100 mi Fecal Streptococcus, No./100 ml Ammonia Nitrogen, mg/l as N Tiedidahl Nitrogen, mg/i as N Oil and Grease, mg/i @:S I)A fo-T C, 20-3) f evit4lV,5 %. LB,4 ri 1;r.ai- c.t,., d;@j= 1.07 IW%OF.O :IMF Comments: White - Fnwirnnmental Enorn.Lab r-a@rv - Environmentalgnarel L-ob Pink - EnvironmentolEngra. Lab aold*nrod - ReauwWs 3MCwbommftw; COPY FORM 10"0-2-PWO Bottle No. oo*w Blar*s: Sample Size ENVIRONMENTAL. ENGINEERING LAD BOD WORK SHEET Date: Dilution Water.& o@6Seed: Description D.O. Bk Ave. I Depletion 5 Day BOD Av iu 7J FC-Jo5 iL fr2@,k@6 *37S.CCO 1>iw PRORM iog$6-2-pwo Bottle No. Blanks: Sample Size ENVIRONMMTAL ENGINEERING LAB BOD WORK SHEET Date: Dilution Water:,eT--g Seed: PDA.*4 Bk Ave. Description D.O. Depletion 5 Day DOD Ave. 7,6 FC 00,3to?o (o-5 </ do 0. eD eP (47 1)00 ratem iolf6a-2-rwo Bottle No, Blanks: Z Sample Size l@ci ENVIRONMENTAL ENGINMUNG. LAB BOD WORK SHEET Date: Dilution Water: Seed: 7 Bk Ave. Description D.O. Depletion 5 Day BOD Av o tj Arm V.-W /,37 139 'FCJo3 4(o7o -e--3J-@(3 I :!;-, -szoo.ooc) 3,2 Y. .2 8@64 atb,& gal.1,(.,6 C</-/YW,6 IV .006 Ito (a IWCADI- '192,n' 1ALA.FGF S @2L 3.0 t -4-x@_r- 't(3 vortmIolioo-a-rwo Blanks: A, ENVIRMMENTAL ENGINMUNG LAB Date:,Zo Dilutionr Wa"ter: BOD WORK SHEET Seed:- --.,,I&eAv,e.,- LBk Bottle No. Sample Size -el Description D.O. Depletion 5 Day BOD Av 7 / .-@e R3 75.:oS. C, 4 ZZA)E -V. 0. 1..5 IbS- :s- 7