Document 6B1D66Qo1RnopEvqw7ovOZ684

Copies to: AR226-2750 E. I. du Font de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine HASKELL LABORATORY REPORT NO. 253-79 Material Tested Ainmonium PerEluorooctanoate Haskell No. 12,037 Other I: a Codes Sample Ready for Testing 1/5/78 INHALATION SUBACUTE I f Material Subm HHUPlastic Produc client, Washington We -ks Introduction; Existing data| 'indicate that Ammonium perflnorooct'.anoatt enlargement following acute i ^posure via inh'Slation or oral routes. This experiment was"des1 effect" level. ause ied t Procedure; Houseline aiT^(-2 K./min) was passed through a cyclone-head dust generator connected to r_e_s_er_v_oi_r which concaine_d_H_R^ The resulting airborne particulate was passed into a 30 1 battery j The atmospheric concentration of|]pB6j^nthe exposure chamber was monitored at 30-min interval fiber filters (type AE, 47 mm). The paniculate concentration was determined by weight gain of the size was determined on a Brink cascade Impactor. SixtyChR-CD'" albino male rats (20/group) were exposed to design level atmospheric concentra 0.08 mg/K.||^._K Rats were exposed, head only, for 5 consecutive days, 6 hr per day. Affter 5 days, a 2-day recovery (weekend) which was followed by 5 daily, consecutive 6-hr exposures. All rats were weighed and observed daily (except weekends) during the exposure and recovery p Laboratory Chow and water were available ad 1tbitum at all times other than during actual exposure Clinical laboratory examinations were performed on 10 rats from each group at 0, 14, and 28 (details in Appendix B). After a total of 10 exposures, 5 rnta from each of the 3 groups were pat at 0, 14, 32 and 42 days post-exposure (details in Appendix C). Select organs were weighed: absolu weights were calculated. On the 5th and 9th dayo of exposure, 10 rats were selected from each group for eye examinatio Company Sanitized. Does HLR #253-79 - 2 Results: Acmosplieric|----^ Concentrations W<0_______ Atjii.' < pheric t S.D. 0.0 (control) 0.008 0.080 0.0 0.011 t 0.005 0.083 0.017 Mean Mas 3 Median Diameter ^ S.I), (u) 0.0 1.63 t 0.70 4.47 t 3.41 Weight Gain; Racs exposed cogBl^showed a suppression of body weight throughout the test. The group was more severely affected during exposure (loss of body weight) and for 14 days of recove Clinical Observations; During each exposure, sporadic cases of blinking, pawing, chewing and re discharge were seen in all groups. Clinical Pathology: Some rats exposed tolflUdust displayed elevated alkaline phosphatase acti were seen in glutamic-pyruvic cranaaminase and glutamic-oxalacetic transaminase after 0, 14 and days. The incidence was related to dose; higher activities were found at the higher exposure le .only the latter activity at 0.083 mg/H was statistically different from controls. ^^f-------- Pathology: Pathologic evaluations showed,cloudy swelling or granular degeneration of hepatocyte of rata (5 of 5 examinrd) exposed ti^----Q|or 10 days with no recovery. Thia effect, was not see ^ longer recovery period. No other compound-related histologic changes were noted. VIPWMH------ [Appendix C.) Organ/Body Weinhts; Various organ weight effects were evident in rats exposed t seen after 32 or 48 recovery days and in the absence of related hiatologic abi compound-related (Appendix D). The dose-related increase in liver weights correlated with existing dat described above. These e ItTes were n and Eye Examinations; 9 No corneal, iricic or conjunctival effects were seen in any of the rats exam exposure days (Appendix E). -irorn^. -^Hi^- P^ - 3 - Summary; Groups of 20_niale rats were exposed, head only, to particulate atmospheres of 0, 0.01,1 or perfluorooctanoatejimn 6 hr /day, S days/wk for 2 wka with 42 days recovery. Rats exposed tofJJUshowed a dose-related suppression of body woi^lit. Rats exposed Co|Cflhad dose-related elevations of plasma enzyme activities for up to 28 days injury or dysfunction war suggested. Liver degeneration was detected at the end of the 2-week exposure period in all examined rats liver effect was not detected after 14, 32 or 42 days of recovery. Eye examinations revealed no compound-related effects. GTH:scg Report No. 253-79 .Date Issued; July 20. 191 Report by: ;-<-- >c_ Sflbrg Toxi Approved by; j.^^T..Lajl JJ. 1I Gerald Chief, Acute In o Sanded. Does" compa^ (.6rj t4TO 13-JUL-7 APPENDIX A <ssssss> 450. GROUTH CURVE-AnPIONIUn PERFLUOROOCTANOATE RATS ^ ^ 1 1 1 1, 1 1 f 1 1 1 1 1 1t i. .^ .L . 1 I1 .1 t 1 . 1 . 1 I1 1 1 ^^ 0) 400. < Ct 0 >-^ -- 350. - 0 1-- UJ >- 300. # AA / ^AA t^*^ ^AA .^/^/A ..-^ <-k . *..^-^ AA* ' ^ .**<A ^"^ A^ . x /flt# / * -' .AA A^ AA < ---- A ^ -' ^ A* ^'1 >l^'^^< * " '^ N N" M * " ->. .^-y...^ l.*..^'""' CD o. 0 250. CL ,*/./ A / ' ,'"" 0 "x / x < ^! 200. 150. I ILE LEOEM)) ( 3NTR -- fOU *< IIOH -- - ., &. ,.. ^--^--.--(^.^--^--?..--y--,--^.^-----,--^--y--r- i i i -^r--r--r--r--r 10. 20. 30. 40. 50. DAYS ON TEST \ ^s.n^.o APPENDIX B SUBACHTE INHALATION OF AMMOBltM PERFLUOROOCTAMOATE BM^|| Haskell Laboratory No..12037 Clinical Laboratory Report Samples of urine were collected overnight (16 hours) from groups 8,of ten rats that were exposed nine times for four hours per day, five * per week over a two-week period to 0 mg/L, 0.008 ag/L or 0.08 mg/L use. Blood was taken from the tail of these rats after the tenth ' ure. Fourteen and 28 days after the last exposure blood and urine (24 hour) samples were again collected from the animals. The examination of the blood and urine included the following: Hematology: Erythrocyte count (RBC), hemoglobin (Hb), hemaeocrit (Ht), total leukocyte count (WBC), relativ; number of oeucrophils Oieut), lymphocytes (Lymph), eosinophils (Eosib), oonocytes (Mono) and basophils (Baso). The indices, mean corpuscular volume (MCV), mean corpuscular hsasoglobic (MCH) and mean corpuscular hemoglobin concentration (MCHC), were calculated from these data. Chemistry: Alkaline phosphatase (AP), glutamlc-pyruvic crans- aminase (GPT), glutamic-oxalacetic transaminase (GOT), ganma-glucamyi cranspeptidase (gGTP), bilirubin (BILRN), urea nitrogen (BUN) and total protein (TPROT). Urinalysis: A measure of the volume (VOL), osmolality (mOs) and pH; a seai-quantitative test for sugar; protein and urobilinogen (UROBL); a test for blood and bilirubin; a microscopic examination of the sediment from pooled speeiaess. The data were analyzed by a one-way analysis of variance (AOV) to compare the exposed animals with the controls at each sampling interval and by a nested AOV for the three sampling intervals. Significance was Judged at the 0.05 probability level. Results; The results of the clinical are sumnarized in Table 1, and for Measurements on individual animals attached to this report. laboratory measurements or observations th statistical analyses in Table 2. are listed in the computer print-out Company Sanitized. Does not contain TSCA CBI Higher than normal activity wasfound cclfKlust for the plasma enzymes AP, after the last .exposure and GPT and GOT in some rats exposed The incidence of elevated enzyme activity was re 14 and 28 days later. lated tothe dose, occurring more frequently in the racs exposed to 0.08 indicated that only mg/LmQlusc. The statistical analysis, however, exposed to 0.08 mg/L was significantly the Sbl activity of the rats elevated. Increased activity of these enzymes is associated with liver injury study with dogs or dysfunction. A similar effect was seen in an-earlier QKHMUCl)' single non-lethal oral dose By These retcsQultsB,Ck chat were given a Chat the livers of rats, exposed inhalation that the effect may persist for dthuesrte, fomraey , besugagffeesctted by the exposure and 28 days or longer. (1) Effect;^ Dogs. on the Livers of Rats and O/^fc 6. K'S^^L. Report by; uW-a1l.t.e..r, aB. Koniecki Clinical Chemist WBK:JRB:ljo Date: October 19-. 178 Approved by: -^L^fL ^L\^j. JoHn R. Barnes Chief .^Clinical Pathology Section Does 1:31 caniain TSCA C'sti CoilipAi^ Saiiii.u.fcc. TA11LE 1 SUCMAKY OP CLINICAL LABORATORY MEASURED EXPOSED TO AMMOMIUM PERFLUOROOCTAMOAT Teat 0 mft/L Recovery I* 26 UeHAIOLCCX Erythrocytes X HeLglobtngX Heortocrit 1 MCVH' MCH ng ,IO,,'/,BB*i 5s .8R66 IS.l ^ " 26 o6 'W30 ^8 46 72 23 o6 t2>5 IA-9 '-7 75 24 MCHC % 33 32 32 Leukocytes X 10/r Nentrophlls H Lyophocycus 1 Eolilnuphlla 1 15.115 18 81 0.8 17.76 22 75 1.2 15.73 18 7t 2.7 Honncytua I Basophlla 1. C11EM1STBY AP 1U GPT IU GOT IU g-CTF IU Blllrubin Hi Ure* altrogeo RX Tofl Protein gX IIH1HALYSIS 1.0 2.1 3.4 . 201 226 270 23 23 25 0 0 068 68 1.7 . 6 2O.J 16 0.9 0.6 21 1.0 0.3 18 6.6 6.7 6.7 Volume al 7 18 14 Oaoolallty OB pit blood, niwber polt!-' Sugcr, number biiw.nuti Protein, nuaber i'inor--1 Uroblllnogen, Ehrllch unit* Blllrubin, nuober positive HIcroicopIc, nuober bnonul 20I9 1327 1706 7.2 7.1 6.7 1.0 0000 00001.0 10001 . 0 Test 0.008 nin/L Recovery 14 28 5.H7 14.7 45 77 25 33 10.77 19 79 . 0.6 1.2 0 fr.12 14.3 45 73 23 32 13.17 19 78 1.3 1.6 0 6.24 14.7 47 75 24 32 12.92 15 81 1.3 2.6 0 242 28 72 1.2 0.3 19 6.3 296 25 74 0.7 0.4 . 22 6.7 291 26 67 0.8 0.3 19 6.8 6 16 12 2337 1490 1818 7.7 6.9 7.3 0 0 0 0 1.0 1.0 0000 0 0 1 0 001 1 . 0 0.08 Test 5.99 l!>.2 46 76 25 33 13.44 20 78 0.8 1.6 0 0 244 27 72 1.1 0.4 20 6.6 8 1624 1 7.9 0 0000 0 1.0 Company Sanitized. Does net  Erythrocytes Hemoglobin Hematocric MCV MC11 MCHC LeukocyCes Neutr.;nhlls Lymphocytes Eoslnophila Monocytes Basophlls AP GPT GOfT gGTP Billrubin Urea nitrogen Total Protein Urine VOL Urine mOs TABU-: 2 SUMMARY OF STATISTICAL DATA F; RATIO OF VARIANCE Tenth Exposure 0.5 1.3 1.1 0.8 1.7 0.1 7.A 0.4 0.7 0.2 0.7 0.0 4.4 1.4 1.5 1.3 3.0 4.8 1.9 2,0 7.4 14-Day PostExposure 1.5 1.7 1.5 2.0 0.0 2.9 4.4 l.b 2.7 0.5 1.2 0.0 2.0 0.8 1.1 0.6 2.1 1.4 0.3 0.3 0.9 28-Day PostExposure 1.0 1.7 0.7 0.2 0.5 0.4 2.2 1.0 2.9 4.9 2.0 0.0 1.0 1.4 5.4 1.8 2.5 1.8 0.4 0.8 1.0 All Data 0.7 2.2 1.8 0.3 1.4 0.4 5.2 0.5 1.4 2.9 1.2 0.0 2.3 2.1 2.7 1.6 6.1 3.2 0.1 0.6 1.0 C^pany Seized. Does net co APPENDIX C Inhalation Subacute - ChR-CD Rats February 27. 1979 Summary and Conclusion Sixty young adult male rats were divided equally into three groups with II Group I serving as a negative control and exposed only to air. Groups and III were exposed to ammonium perfluorooctanategfHIIIyat concentrations of 0.01 and 0.08 mg/1 respectively, 6 hrs/day, 5 days/week for 2 weeks. On 0, 14, 32, and 42 days post-exposure, 5 animals from each group were sacrificed for pathological evaluation. At necropsy, the animals were examined grossly, and the following tissues or organs saved for hisiopathologic evaluation: external ear, abdominal skin, trachea, lungs*, heart*, thyroid, adrenals, thymus*, mediastinal tissue, spleen*, sternebrae with bone marrow, stomach, small intes tine, large intestine, liver*, kidneys*, testes*, epididymis, brain and eyes. The results of pathologic findings are summarized in Table I. As the table shows, an apparent compound-induced toxic effect consisting of a cloudy II III swelling or granular degeneration of hepatocytes was detected in the livers of all five rats (5/5) in both Group (0.01 mg/1) and Group (0.08 mg/1) at the end of the rwo-week exposure period. However, the hepatocoxic effect was not detected after a 14-day or longer post-exposure recovery period. All of the remaining pathologic findings listed in the cable are considered to be spontaneous or the result of intercurrent disease. * Organs weighed HHCC:WCK:mjb Report bv: -7^ (Uj^. X^?. Bans H. C. Chen, D.V.M. Senior Research Pathologist Approved bv: /. [/,//^y/j^,W^ ,,, (^ W ill1 i-ai --m_ ^ C. /y' //VVT^-jFL'J^.UW^^ K_r--a--u--s--s, DT\ .VTT .M . Chief, Pathology Section Company Sanitszsd. Does not contain TSCA CBJ TABU; i BIIMMAIIY OF rATiioLOisic rmDnr.s IN HAI.E cim-cn BATn EXPOSED TO /UMOHIUH rEnnjJOIlOOCTAHATE Animal.No. R pViV.i i j - 8 n I 0 ,. ^ d 3 " h i u h ri 58 5 d"<v iopkR "tia v a8 g H^ dJT< l! I It u gi b n. S 8'^ g s 4 " KSl a T<T K "; V " Pi)i p> ft - fj ** 5 8 II ti ^li Mii i? JBl 'h rl ? .tih 1" 3 P. < s fl "u p< M ia ^Is' ^a aS? ^B s^ ' 11 y p ' lr l a- 3tf &) ft" 'a i! f1 d S' Q c u i1 ttii n '-< ' -a ay a tta an>a ,3*> Ha 10 0 s i i R yi.W-.h I 0 10 0 1 i - ~ - i i R ?^5<>S R ??fi'ft> R ??6?f>7 R S^55B R 2?('?';9 I 0 T 0 T. 0 I 0 T 0 10 0 s 10 0 2 10 0 3 10 ll 1 10 lh 1 i - i - - - - - - - - - - i i - - . R ?76?f0 I 0 10 14 1 i - - - s R ?^6l R ?^fi3 I 0 I 0 10 Id a 10 I't l i - - i i - R 2^5"55 I 0 10 52 i i R ?5<>Ti' I 0 10 5S l i Code: -< (to legions 1$ very mtlid change S: Slighb change the tinnnc not avallnhle for tilRtologIc evniuatlon --..-- - - - - 5j Moderate change Pi PreBent ^4 g|^a'& * --1 1 Ul T C -< 35|ff 0 0 3 .s.sas3. u 0 u> tfl h (- O 4l - - - - - - Xi (Iroe Company Sanftized. Do ? & ;aaaiaaaaaa:gaaa a i\^7r>^y>'\~\?>-^^y>S -*Tal 033303 iu>-0'a3'3\nse a :u >j sji ji u J4 3\ vjl -o i\i ji ji <J> ^Jl .- -3i o -a M sj4 j< .1 ji -a M M \ \> -- j< j< j< -ji a -I -.rf '^N '^ ^* J 'J* J* 'J* M <J1 sfl sfi 5" .=" J \^1 ,9 1 a t -4 ' j< c3 ifI ^it^M w--I wq iI -4 M >4 -1 X |QlOU? ' -- -< 1-] >-1 - -- oooo ooooo 1 33 ooo ?~ S S :? ISOM (ag/1) 1 i3 t 3333J5ooooSoS 2,& ^..a' C&ya on teat 11 a 3 sS a3 a9 ^ft? SU ,,M^ ^f0 S M ^M 3 ?;;3 '5 .'U aSSeWeoVVwiyy SSsJyS g -IM 3" OJM-M eg ? iiii M N i>, 31 ' 1 111 11 njMi-'h^Mi-i^k' Lung: Chronic maTin* pnetZBOBia, eirly jtage l i Luag: Focal alveolar iiiiiiii . a ospbyifBA i ^ 1-1 H i Uwrt Bon-^tcilc Bicro-grKBuloBa/foci of >m^nT-r heoonolejii Livr: Cloudy veiling/ 111 11111 Sraiuilar iageimtlon of of hcpfttoc^^c^ "< ; ^ 3 . o p S?a j^ 3 E 3 E3 I01^ i 5 |:3 ; 31 H V u-wr; ?ocal pcriportal 5 g trad&tls | ai i i i i i i i i '3 Ll-nr: foe&l perltonj cla 11111111 irt.tl lobar adhesion i i i i i- i i i i ,-> i i Kidney; Chronic ?rogre- 3 - alT> rrphroala, ewly S atae 3 i i i i i ry i i i i i i Kidney; ^srdronepbrcala, 1 unilateral 3 i i i .1 i i i Tesces: Focal atrophy/ "" degneraclon of sendal- faroua cubules x a iiiiiiiiiii & i i i M 11111111 a Taatia; Tartleulxr acropny^heaa, unilacerai Thyau: Atrophy External aar: Focal aec- 3 iiii i i i i . i "lyils af cart,LL;;e early scaae af dsfochon-' 'droaatosia 11 iiiiiiii i'4eatc: Chronic focal nvocar-iicis ^aro^aiua^roa^iau^iaa^rao'a^naa^'ya^wa'^VBM^-M.^-ra^oMa^ a a '^M."O^ g K 3 j - i ^S ^S ^ '^ ^ ^3 ^ ^ ^ '^ ^ a 2 ^3 ^3 s " |^".o^6&~l^^<n^^^'-loo 3 s|!l^rt|t4-l(_l-4'>M4MM>-4l1---ll>-4l--MIl-ll->|l^-l'^^l'^-4"i<4 " t-s !p^^P ^ So9o'o9oo9o9oofo!oio3oohoo"o^afooi^ooipo^>po-po" p0*p0 i-'t- pose rag/I) & S ooooooooooooci 5 s bays on te.t Si^^i^^^iS-iS !?&'? & o P^o^y days m\ iji-if'h-nj^i-ii-it-'rorM-oroi-i M Lung; Chronic aurine pneuaonia., erly stage , ^ 1< iiiiiiiiiiiiiii h-h'h.^.^.i-i-i^.-;^-! " i Lung: Focal alirol*r eapfcyeBa j i ^ UVT": Heft-specific adaro-l graBuloua/faci o: aKtra- 1 wdallcy bcnaeolaii.s i i i i i i i i i 1 < i t i i ^ Ll-wrs dcudy swelling/ grunjar degenes&tloa of >estoc7te . I^iiiiiiiiiiiiiii i i i i i i i i i i i i i i i i Liver! Focal flerlportil tnditia i Liven Pocal perltonlila Tith lobar adhesion 3 wi-i i-> i i i i i_> i-< r- ^ ci i_< i t-i i c- .idney; ChTonic pzogreBaive nephroeia, early 0s1- atage a i i i i i ro i i i i i i i i i 'g a i Kidney: Wrw.sybxwi.s, aoilaceral 3111111111111111 i x a: iiiiiiiiiiiiii ^l i < i i i i . i 11 i i iiiii i i , 1,111111111 , 111111 iii 11 111 r i Testes: Focal atrophy/ degeneration of seaioiEerous rubules JTeatia: TeBtlcular acrsBhy head, ina.laceral ThTBUA: Atronh, Excernal ear: Fecal nec- roiysis of .sartll.w, early stage -it isteochondromatosis 'Hear;: Chronic focal -vocariittis i |8 - r" s"3 JS 25 =; JS I" 3 S v |_ "' |' 3 1 q - M 3 ^ FART IV TABLB I OIIMMAHT OF PATllOljOOIC FIHIlTWiB TN HUiF. L'lm-CR BATS EXroSED TO AWOMIW PCTFmOKOOnTAHA'fE ^ a; 1 Aninnl Ho. a 5 --.. - g -a----*---3-- 3g ti ,,& a I ^?tj&jg 5 5 ^J58 s1 B^ f ^ o- 0^5 u u a V ^ too D fi P "p P 0 g- K if a l!-s! S- K *! 'J - ^ Oe-TnJ f -<jQ 0 M 01 1 ^l fj "1 |^1 S : 1. |ug.^-3^ a EH}S CT & V ff 6 ? fc a a u " n 0 ! Q p t0( .T- o r i< ^ f> fi ' u in HI C 3 1 t sl Jllll 1 I? I g S -a ? ar ^ IJ 01 0 in u- h s d^^ di] nS IT$ a 1 U . ,fci.-g. H P'<.ll0' ITI n.on 10 0 1 H ?y'iioi m 0.00 10 0 1 5 1 - .- n s-^iin^ III o.on 10 0 ? 5 - - - p Wior, P 2:">fif|07 n s?^9B B S^W B 25^100 R ?5C'iioi B 2?6h02 Code: -; thp MFISIIP Trr 0.08 10 0 S III 0.08 10 0 S III O.OB 10 lit 1 III 0.08 10 lit 1 III 0.08 10 lt 1 III 0.08 10 Ib 1 III 0.08 10 lit 2 No lesions I: VIsry i"lld chanRa ?! not nvnl|nb4<> for lilntolnnlc rvniuntinn 2 Rllpht 1 2 - i - 1 2 - - - - - 1 - - i - 1 - ,, p. 1---1.-- 1 - .. - - - 1 - ,, - - - change 5 Moderate ctiange r; IBlon present X Company Sanitized. Does no PAHT V TABLE I auwART or PA'nioKxnc FtNiiiNus in HME cim-cn RATS EXFOBEP TO W10HIW rERFUJUROOCTAMATE ? Animal No. 0 R 216195 III R ?1^19' ni B ?5ft19'5 in R 25<W' . HI B ??<'.?97 ITI R 216588 in B 2Vi509 TTT n 256590 ill R 216191 III R ?1?;592 III | li 8 0.08 0.00 0.08 0.08 o.oB 0.08 0.08 0.08 O.OB 0.08 1P. 8 8 ? J I1 10 52 10 12 10 52 10 52 10 52 10 !? 10 4S 10 iia 10 h3 10 li;? I? a; y a Q 2 2 P 1 2 1 3 1 X 1 a 1 1 f 1 ' 1 H i tuigrlgd 'J-^j M? lb o ij "?fi U1(H0 fi & s^^F vf m 4-1 d(i o 5 ?j^J8 KaSKB ?;? 5 aft'o *) R >} 4J i - l i - - i - - i - - i - - i - - i - - i - - i - - i - - . ' n K,S d'S 5'-< S5 l?g ?hq5-sS 3 R dd ^ a ,1 S - :} ",} [ U I 1 ^?;1^ n " Fn l&g fe>f la i 1 S . - n ?^ ?arj P - - 1 - - - 2 - 1 - - - 1 - 1 - - - 1 - "?.^ " C C QJ 2 CI;T< " <" a * '-< 0 0) It u c r-i o o3 b v4 J3 " 3 u S^ ri ^ f b B a -4 U . --i; a a f^ 5 ^ "mono ti n ..) 1-1 m m u C3 in 03 ki 1 1 U ,, >? n x; T< D. 5 M U -<" ( n - - - - -' i Code: -! No leHoM li Very nlld chanRe 2i Slight change 5l Moderate change r: Leclon preBent X: the tIflBuc ott nvallnhftc for hIslnloRlr evd hint Inn Company Sanitized. Does not conta ^'^^B"S APPENDIX D MEAN ABSOLUTE ORGAN AND BODY WEIGHTS AMMONIUM PERFLUOROOCTANOATE VIA INHAL. OF MALE RATS^I <SUBACUTE-6;f||||^| ' .'-:" . ^ C -:?:'. -ceuuE'- I CONTROL IIIII LOU LEVEL HIGH LEVEL F RAT3CX1) LSD<2) DUNNETT(3) UHS < A) -EItlAL-BU 273.6000 258.8000 239.0000+ 6.125* 21.6130 24.7992 246.0000 .----.-.-G&QUE. -KIDyEY. J CONTROL IIIII LOUI LEVEL HIGH LEVEL 2.5560 2.2840+ 2.2:180-* F RATIO<1) LSD<2) DUNi<(ETT(3) UMS < 4) 5.9205K 0.2269 0.2603 0,0271 <1> RATIO OF AMONG- TO UlTHIN-GROUP VARIATION--ONE-FHCTOR ANALYSIS' ^m^^i^l ^^lil^^;^ <2) LEAST SIGNIFICANT DIFFERENCE--GIVEN H SIGNIFICANT (ALPHA=0. OSy^.R^R^piaSpl"^^ ANY Tyn MEi^NR GIFFER1MG BY MORE THAN THE LSD ARE SI5NIFICATLY rsIFFERE^Tl^^^^ kITrh A VARIABLE-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. m THAN WTTi-i "%^ DUNNETT TEST--ANY TREATMENT MEA'l H1FFEPINO FROM THE CONTROL MEAN'S'Ka^^E;^!! THE CONTROL ^l^iljp ^III THE HUNNETT STATISTIC IS SIGNIFICANTLY DIFFERENT FROM -- A VARIABLE-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. (-li kl; THIN-GROUP NE(4N SQUnKF . + SIGNTF-ICANfLV DIFFERENT <p<0.0'5> FROh CONTROL GROUP BY LSD. < IG-'II-1CANTLY DIFFERENT (P<0.0^> FR'3n CONTROL GROUP BY DUNNETT TEST^^gjgl^;!;^ * S:1'NIFICANT AT THE 0,05 FROPAPIL :[ ! Y LEVEL. * MEAN RELAT IUE ORGAN AND BODY WEIGHTS3 TO AMMONIUM PER FLUOROOCTANOAT E VIA INHAL. GBQUE------.------ ,,-- --HatF. tAatBt.*I--- ---------- I CONTROL II LOU LEVEL HI HIGH LEVEL P RATIO<1) LSD<2) DUNNETTO) UMS(4) 0.3548 0.3243 0.3254 1.725 0.0406 0.0466 0.0009 LUNGS----- 0.6206 0.5447+ 0.6083 4.569* 0.0587 0.0674 0.0018 F(SOURBAMCAULETER-Cfi^) TSRECEXOPVOERSEYD DAYISjy ;'.H LIUER-----..SELEEN---- ..? "v^, :|$iDyEX-^ ' 4.7259 4.9997 5.9226+ 02075 0.1665+ 01455+ " ;b.9348;| 0.8920% 0.9292:^ 4.316* 0.9299 1.0670 0.4554 17,638i; 0.0232 0.0266 0.0003 0.273^ 0.1372^ 0.1574;H 0.0099;| ;5 ,6 ^ "'?? .^' . -GE:OUE.----.---------- IESIIS----- IciXdua------ '; III CONTf.OL 1.0253 0.2418 ;; LOU LEVEL 1.0059 0.1808+ HI HIGH LEVEL 1.1105 0.1438+ "I^^-^^^1^ ^^^ri^^"^^'^^^^^"0' F RATIO(1) 1.836 7*713* LSD < 2) 0.1266 0.0549 DUNNETTO) 0.1452 0.0629 in3<4) 0.0084 0.0016 ,1, ..TIO OP ^ONO- TO W1THIN-GR03P ^I.TION-ONE-F.CTO. ^LYSIS 0? ^I.NCE <2> L^ST S^IFIC^T ^ ---- or o.cs. ^ S ^ ^ ^ ^ z ^ ^ ^^^O^O^OL^r <3, BU^TT TEST-.HV TP..TMENT ^ ^^ ^ ^ S ^ ^ ^ ^ S S (4 UITHIN-GROUP MEAN SQUARE. ^ ^ '- 0-- , cin.TFTnANTLY DIFFERENT (P-::0.05.) FROM CONTROL GROUP BY LSD. SIONIFIC.NTLY DIFFERENT <P<0.05> FROM CONTROL GROUP BY DUNNETT TEST. SIGNIFICANr AT THE 0.05 PROBABILITY LEVEL. M^L .-:.^,..fc^.,a-^jaiSa^a^^ Con^ Saniti^d. Does not contain TSCAC6L MEAN ABSOLUTE ORGAN AND BODY WEIGHTS OF MALE RATS EXPOSED TO AMMONIUM PERFLUOROdCTANOATE VIA INHAL. (SUBACUTE-14 RECOVERY DAYS) G&OLP I CONTROL IIIII LOU LEVEL HIGH LEVEL F RATIOd) LSD(2) DUNNETTO) UhS(4) Elbli&L U - - - - -..HE^fil- -.--.- LL1UGS---- 341.2000 341.0000 303.9000 1.1660 1.1720 1.0480 1.8480 1.6900 1.6640 2.604 41.1241 47.1867 890.6333 1.810 0.1601 0.1838 0.0135 1.226 0.2771 0.3180 0.0405 LIUE6 - 14.4980 16.0560 15.0360 0.684 2.9474 3.3819 4.5750 SilLEEhl ^8 - ^ 'i:- ' .^Q . 7 5 ' 0 0'-^i^ 0 .6260^ 6 .58601 ; ^3 3.0291^ 0 .151.4ii 0 . 173'7^ "0 .Ol2.iw i ... -.-..GROUE-..-..-..-....- , -- -......-.KIDNEY--..,..-.- . -- .T1.1C--Ci3TI-TAi6.3; " -- -- -..-.-lyjcuus.--. - I fOMTROL LOU) LEVEL IIIII HIGH LEVEL F RATIOd) LS-JLI < ? ) DUNNETT(3) UMS<4) 2.8520 3.0060 2.6360 1.793 0.4278 0.4908 0.0964 3.2760 3.1580 2.5840+ 3.949* 0.5740 0.6586 0.1735 0.6040 0.6520 0.6020 0.250 0.1743 0.2000 0.0160 (1) RATIO OF AMONG- TO U:l THIN-GROUP VARIATION--ONE-FACTOR ANALYSIS OF VARIANCE (2^ LEAST SIGNIFICANT IHFFERENCE--GI*.'EN A SIGNIFICANT <ALPHA=0.05) F RATIO, ANY TUO MEANS DIFFERING BY MORE THAN THE LSD ARE SIGNIFICATLY DIFFERENT UTTH A VARIABLE-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. <3) THAN WITH DUNNETT TEST--ANY TREATMENT MEAN DIFFERING FROM THE CONTROL MEAN BY MORE THE DUNNETT STATISTIC IS SIGNIFICANTLY DIFFERENT FROM THE CONTROL MEAN A UARIABLE-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. (4) UI THIN-GROUP MEAN SQUARE. + SIGNIFICANTLY DIFFERENT (P<0.05) FROM CONTROL GROUP BY LSD. * SIGNIFICANTLY DIFFERENT (P<0.05) FROM CONTROL GROUP BY DUNNETT TEST. :: *" SIGNIFICANT AT THE 0.05 PROBABILITY LEVEL. Comply Sanitized- Ooeso^cor!faiti,;ISi|ACB^ J - ''--ssssssssK-Sfe MEAN RELATIVE ORGAN AND BODY UEIGHTS FOR MALE RATS EXPOSED TO AMMONIUM PERFLUOROOCTANOATE VIA INHAL. (SUBACUTE-14 RECOVERY DAYS) -GBQUE, I CONTROL IIIII LOU LEV;EL HIGH L-EVEL F RATIO(l) LSD(2) DUNNETT<3) UMS(4) UlTAC-T 0.3420 0.3438 0.3458 0.029 0.0339 0.0389 0.0006 LUNGS 0.5392 0.4965 0.5514 1.952 0.0637 0.0731 0.0021 ---- LIUEE sifLEEtsl---- -K3;lh!Ey-a .: '";. ' - -^ 4.2487 4,7164 4.9095t ' 0.21BO 0.1838+ 0.1925+ : W.83A&IJ OS. 8837.; 0*8647 a : ^i 3.350 0.5720 0.6563 0.1723 5.090*0,0243 0*0279 0.0003 ' .0.62411 ^0920? O^IOSS^ ^.0045' .BKOUE- -iE3iis.--.---iyYaus- I CONTROL LOU LEVEL IIIII HIGH LEVEL F RAT 10(1) LSIK2) DUNNETT(3) UIMS<4) 0.9658 0.9263 0.8528 1.004 0.1763 0.2023 0.0164 0.1773 0.1916 0.2003 0.408 0.0560 0.0642 0.0017 (1) RATIO OF AMONG- TO UITHIN-GROUP VARIATION--ONE-FACTOR ANALYSIS OF VARIANCE (2) LEAST SIGNIFICANT DIFFERENCE--GIVEN A SIGNIFICANT (ALPHA=0.05) F RATIO? ANY TWO MEANS DIFFERING BY MORE THAN THE LSD ARE SIGNIFICATLY DIFFERENT UITH A UARIABLE-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. (3) THAN WITH DUNNETT TEST--ANY TREATMENT MEAN DIFFERING PROM THE CONTROL MEAN BY MORE THE DUNNED STATISTIC IS SIGNIFICANTLY DIFFERENT PROM THE CONTROL MEAN A VARIABLE-UISE FALSE POSITIVE (ALPHA) ERROR RATE OF 0.05. :: <4) UITHIN-GROUP MEAN SQUARE. : + SIGNIFICANTLY DIFFERENT (P::0.05) FROM CONTROL GROUP BY LSD. * SIGNIFICANTLY DIFFERENT <P<0.05> FROM CONTROL GROUP BY DUNNETT TEST. 'M.&TI TTV i FUEL. '-C-^o(m^p'^a"n^yBS.aSfit^itMSf^fl-iafef'cg.e-aQ-a^'^^W^^r'.C^t^'eM^aHsaala 5W6B ^S ' -^f'.- '}':v'^^'y;^^.^'iW"?-it'ii^sa<"Ti'e?t'^.M^^';y1i: "^i-aa i '^| -M MEAN ABSOLUTE ORGAN AND BODY UEIOHT s OF MALE RATS <8UBACUTE-32 VIA AMMONIUM PERFLU OROOCTANOA rE INHAL * - ^ n&niip I CONTROL IIIII LOU LEVEL HIGH LEVEL F RATIO(l) LSD(2) DUNNETT(3) 1MS(4) EIH6L-BU- - 367.2000 361*6000 351.6000 0.494 34.6587 39.7681 632.6000 ----UE6RI-- - --LUNGS------ LlUEfi-,: 1.1100 1.1260 1.1340 1.7940 1.6840 1.7600 12.244^ :' ^^a-^r 12.5760; 0.054 0.1628 0.1868 0.0140 0.575 0.2289 0.2626 0.0276 . 0.099' ;::.^fc?rf?.*'?i3!^ 1.6240 1.8634 .;^105A 1.3889 "''^ig 004^1 ^.'^'fgl . .^i^ . ------------------- -- -.GSQUE- I CONTROL 111 'I LOU LEVEL II HIGH LEVEL - -- .- KIDNEY-- 2.6740 2.8200 2.6660 .Tj.Ct-"aCxT^TBC--- 3.0520 3.2400 3.1060 ----IUXBUS--- 0.5900 0.5220 0.6340 .,:'' '.; ''"M^i :.' i^ag ' ----;%:1^| III F RATIO(l) 0.279 0.891 0.3159 1.816 0.1290 LSD(2) DUNNETT(3) 0.3058 0.5803 0.3625 0.1480 ^.'.';^ ^N UwMnaS\(4 )/ 0-.._13_ 4. 7 0.0526 0.0088 .^'^ ':s ^s VARIATION--ONE-FACTOR A--N..A. LYSIS . ^OrF. ;v|ii[ANCE:| "-. ^. ^.? ' 'n^t-.a'AMr'C"^ . . ,, (1) RATIO OF AMONG- TO UITHIN-GROUP (2) LEAST SIGNIFICANT DIFFERENCE--GIVEN LASDSIGARNEIFISCIAGNNTIFIC(AALTPLHYA=D0I.F0F5E)REFNTRA^Hlb !3 AUNITYH TMAO VMAERAIANSBLDE-IFUFIIESREINFGALSBEY MPOORSEITITVHEAN (ATHLPEHA) ERROR RATE OF 0*05^ TREATMENT MEAN DIFFERING FROM THE CONTROL MEAN BYltWRE MEi?iN T(H3A) N DTHUENVNADERTUITANNBTELETETS-UTS--ISTAEANTYFISATLSICE PISOSSITIGIVNEIFIC(AALNPTHLAY) DERIFRFOERRERNATTEFROOFM 0T.0HE5. CONTROL ' ;:;| WITH A : I (4) UITHIN-GROUP MEAN SQUARE. + SIGNIFICANTLY DIFFERENT (P<0.05) FROM CONTROL GROUP BY LSD. * SIGNIFICANTLY DIFFERENT (P<0.05) FROM CONTROL GROUP BY DUNNETT TEST. , .':!!i -. -t TSCACBJ Company Sanitized. Does not contain .;;ii.a,:a5^,^S.,^,.,,.-.. HP '^'T^SJB ORGAN AND BODY WEIGHTS FOR MALE RATS EXPQsi&^IS^SI RECOVERY; Bilt^iil MEAN RELATIVE PERFLUOROOCTANOATE VIA INHAL. (SUBACUTE-32 " .'- Vi -:'. -^A^-w-.Kg^S^ TO AMMONIUM ._-.----GBQUE:----_- I CONTROL IIIII LOk) LEVEL HIGH LEVEL F RATIO<1) LSD(2) DLINNETTO) UMS<4) -UEAKI-- 0.3028 0.3104 0.3221 1.379 0.0255 0.0293 0.0003 0.4884 0.4662 0.5004 1.449 0.0443 0.0509 0.0010 3.3384 3,4328 3.5735 1.472 0.3004 0.3447 0.0475 0.1789 0.1661 0.1704 0.765 0.0231 0.0265 0*0003 '^M m^ '.:W3r4tg . W ^ i -.SfF567;;! ' ' . ; . ^ ':'"'":';^KS?:i'-3" ^r-s. ; -^6s3s .;:.;>31&3_ ;8. -0. .4. 1 ..;^w'^i2--6"r^ ';.;.,!,<&!,, .. ,, ,':^t -? .IESIIS- .laXBUS-- .--.---GEs.aUE:--.---------. I COMTI-<01. LOU LfcyiE-.L II HTGH L&'yFL 0.8336 0.8991 0.8849 0.1627 0.1439 0.1810 III F RATIO(1) LSD'2) 1.067 0.1029 0.1180 1.913 0.0413 0.0474 DUMNETT(3) UUIM">SS(44,>> 0.-.0.^0-5-_6 0.000'? VARIATION--ONE-FACTOR ANALYSIS OP VARIANCE. .1) RATIO OF AMONG- TO UITHIN-GROUP (ALPHA=0.05) F RATIQ.- LEAST SIGNIFICANT DIFFERENCE--GIVEN M SIGNIFICANT LSD ARE SIGNIFICATLY DIFFERENT THE ; .2) DIFFERING BY MORE THAN POSITIVE (ALPHA) ERROR RATE OF 0.05. Alo N1:1/ l-l iUftCi VMAEFAIMNSBLE-UISF- FALSE CONTROL MEAN BY MOF.-E DLINNFTT TEST--ANY TREATMENT MEAN DIFFERING FROM THE DIFFERENT FROM THE CONTROL MEAN 0)UTH:[ATHN TAHEV: ADRLIiANtNfELTET-USISTEATFISALTSICE PISOSSITIGIVNEIFIC(AALNPTHLAY) ERROR RATE OF 0.05. i , (4) UITHTH-GR-01JP MEAN SQUARE. ' DIFFERENT (P<0.05) FROM CONTROL GROUP BY LSD. + SIGNii-](:p)N(|..'r CONTROL GROUP BY DUNNETT TEST. < Sir-mFICANTLY DIFFERENT (P<0.05) FROM S^.Oo.no.co^TSC.CB, Company ....-aK.l.iaiiiil-fea^^!?, ' " ' ' ' ...-.-...,----'-"^^"-p--"^^-- -.^^^ai.s^s^g^ E ^ ^ AMMMEOANNIUMABSPEORLFULTUEOROOROGACNTANAONDATEBODVYIA WIENIGHHATLS. O(SFUMBAALCEUT-R42ATSREC^^j| ____-GE;OUE--------------EHit61--BU- I CONTROL IIIII LOU LEVEL HIGH LEVEL F RATIO(l) LSEK2) DUNNETT(3) UM3(4) 427.8000 409.8000 372.8000* 3.4A9 46.3909 57.22^B 1133.3667 .UE6&I- 1.3460 1.2420 1.1640 3.078 0.1604 0.1840 0.0135 -LUaGS------ 1.9080 1.8920 1.8480 0.035 0.3275 0.3758 0.0565 JjEOUE- _KIDblE^- -ICSIIS-- ...lU^CtiUS-- I CONTROL LOW LEVEL iIIn HIGH LEVEL F RATIO(1) LSD(2) DUNNETT': 3) kiM&'<4) 3.2100 3.2220 2.8500 1.241 0.5846 0.6708 0.1800 3.3000 3.2560 3.2100 0.235 0.2862 0.3284 0.0431 0.6040 0.5920 0.5440 0.540 0.1331 0.1527 0.0093 (1; RATIO OF AMONG- TO UITHIN-GROUP VARIATION--ONE-FACTOR ANALYSIS^ CH||ip|:IAN;l A STGMIFIC:(">NT (ALPHA=.0.05) F^IIN^O' ' (2) ANY LEAST SIGNIFICANT DIFFERENCE--GIVEN TWO MEANS DIFFERING BY MORE THAN THE L&E! fSRE SIGNIFICATLY Or DIFFERED "^M yi-"!-! A VARIABLE UISE FALSE POSITIVE (ALPHA) ERROI- RATE 0.05. (3) THAN UITH DUNNETT TEST--ANY TREATMENT MEAN DIFFERIf.'Q FF<OM THE CONTROL MEAN ^Y^CiW PROM THE CONTROL; .MEAn ATHEVADRUIANBNELTET-USISTEATFIASLTSICE PISOSSITIGIVNEIFIC(AALNPTHLAY) DE.R' RF-OFRERER.NA'Tl E OF 0-05. ^^l ;^ (4) UIITHIN-GROUP MEAN SQUARE. + SIGNIFICANTLY DIFFERENT <P<0.05) FROM CCN f i":OL GROUP BY LSI:'. ^.^A * SIGNIFICANTLY DIFFERENT (P<-0<05> FRCiM C(!?-T^a!. O.ROUP BY HUNNET't ;"ESI^||, Coinpany s^^'^^^Sl -- ^ MY|| MEAN RELATIVE ORGAN AND BODY UEI6HTS (SFUORRACMUALTEE--4R2ATSRECEOXPVOERSEYD UA PERFLUOROOCTANOATE VIA INHAL. TO AMMONIUM "^Mn ; SfS^ : '.jg -- - G&QUE ----- --------UE6EI-- -- 1 CONTROL IIIII LOU LEVEL HIGH LEVEL F RATIO(1) LSD(2) DUNNETT(3) UMS(4) 0.3162 0.3029 0.3125 0.405 0.0333 0.0382 0.0006 -LUNGS- 0.4451 0.4629 0.4950 1.530 0.0629 0.0722 0.0021 --.HUEK- . 3.8684 3.7516 3.6035 0.573 0.3807 0.4368 0.0763 SELEEH--...--. .SWsISUl^^-^.H,^i . 0.1847 0*1613 0.1.875 '^.719<SJl| .^0.7855|| ;!'y^,7&2QSj 2.089 0.0307 0.0352 0.0005 -[Q^ ^^Rifi ; . : r;;U SO^-SS "0.0904!^ ':' 0.1037-1 0.0043l .bKOUE- .IESIIS- .lU^ttUS- I CONTROL 0.7745 0.1422 i LOW LEVEL 0.7982 0.1451 II HIGH LEVEL 0.8630 0.1456 ^ III F RATIO<D 2.687 0.027 LSIK2) D'-INNE-TT (3) 0.0861 0.0988 0.0345 0.0396 UMS(4) 0.0039 0.0006 W)O1F\J \ AifMONG- TO UITHTN-GROIJP VARIATION--ONE-FACTOR ANALYSIS OF VARIANCE; (1) l':ATIO A SIGNIFICANT <ALPHA=0.05) F RATIO ;; (2) LEAST SIGNIFICANT DIFFERENCE--GIVEN BY MORE THAN THE LSD ARE SIGNIFICATLY DIFFERENT AUNITYH TUAO VMAERAIANSBLDEI-FWFIESREINFGALSE POSITIVE (ALPHA) ERROR RATE OF 0*05. DUNNETT TEST--ANY TREATMENT MEAN DIFFERING PROM THE CONTROL MEAN BY MORE FROM THE CONTROL MEAN TU(H3ITA) HN TAHEVADRUIANBNELTET-WSISTEATFISALTSICE PISOSSITIGIVNEIFIC(AALNPTHLAY) DERIFRFOERRERNATTE OF 0.05* . (4) UITH1N-GROUP MEAN SQUARE. + SIGNIFICANTLY DIFFERENT (P<0.05) FROM CONTROL GROUP BY LSD. * SIGNIFICANTLY DIFFERENT (P<0.05) FROM CONTROL GROUP BY DUNNETT TEST* Co^pani"*1' S:i an"is^c. Dcs'"s-' wnos;,ic^amnlwain TSCA CB'.' Procedure: Sixty ChR-CD young adult male rats were divided in^|;( three groups of 20 rats each and exposed (head-only) six houa;^^^^ day, five days per week for a total of 10 exposures over a two-;'^?]! |' week period to O.OC, 0.01 or 0.08 mg/1 octanoic acid, perfluoro-^f ammonium salt paniculate. for eye examination on the Ten rats were selected from each grolig 5th and 9th days of exposure. ObseirvtG-; ; tions of the cornea, iris and conjunctiva were made with a hand-"^; : slit lamp and a biomicroscope after using Fluor-i-strip stain, ^ ';: Results: The results of the eye examinations for each rat are ; presented in Tables II III. halation to octanoic I, acid, and When rats were exposed by in-^;l; perfluoro-, ammonium salt particulate;at^ ^^ 0.01 ag/1, two of ten rats at five days and five of ten rats at ni ex ne po s da ed y s to h ad 0.0 d 8 r ied mg/ 1 r ed of di t sh es t b ro m w a n te disc rial, h arg two e a of r oun ten d r the ats ir h a e d ye d s. rie d IW ^ I ie ^ n reddish brown discharge at five days and five of ten had brown dits-y charge^and one had dried reddish brown discharge at nine days. ; ^ This discharge (chromodacryorrhea) is often seen in animals under stress. No corneal or iritic effects were seen in these rats. : No corneal, iritic or conjunctival effects were seen in the control; rats at any time. Summary; No significant ocular effects were seen in rat eyes when examined after the fifth and ninth exposures to octanoic acid, perfluoro-, ammonium salt. Report by; ^fcua-^ fjlLs Doris F. Edwards Toxicologist Approved by: ^^fGYera^ld,^^i.. Kennedy Chief, Acute Investigations Section >DFE;scg ite Issued: *^ June 2r, 1979 contain T5CACB1 Company San^d. Doa..,ot -.--;. --.'tMSiiJ?.'-^. -r?-wyw/-^f.a - TABLE I ^ Conttrol (0.0 mcr/U H-12.037 ;f 143 CSW spaci-iy araa ^i Csaiuse-sy/a fdsw silaaasia diaei.ar;* riaor-t.-a^i?9 Steals e5 esr-.sa 3S Csfxea ape-sy W Csaluaes.i.va rdas3 CSCW1.S i-ie.-.Arg* Tiao.c-i.-atri?5 3-esi.e ci ear-in* Is50 - Cassfti opteisy &c-- 1?-3 Caajuas^va c*daaa ctMBoaia disc!&f9 Fluec-i-atr;.?3 3&3aic =S e3ra .6351 Caca-- Bpc:.=y ara ^s. Caa^'sacs^v rdaa eifBoai.j Aiacirrg* riaor-i-ttr:.?' Siesic e; saraw ;63S2 & ^ tezaea o?acs.t7 r*a Iri.* Cony-nc^iTa c!a* sisjmosi.j dijehAf;* ?laor-s.-^i.p* Steals e5 cara S ftJiys a/'4 i* -3- 3 0 0 0 3 0 0 a 3 0 1 3 9 0 3g ? te v 9 11r/ 9/1 0 3 3 3 3 3 0 3 0 ng 3 0 0 3 ag 0 3 a 0 3 0. 3 0 3 3 0 0 0 0 0 0 0 0 0 9 a 3 0 Q 0 0 0 5 =? nq - n^ te -3-- L a 0 3 0 0 0 3 0 0 3 0 3 0 3 0 0 3 0 3 0 3 r.g n? u a 3 0 0 0 0 0 - 0 - cg to a 0 0 0 0 9 3 0 C 0 0 0 3 a n9 ~~3. ." S S 0 3 0 3 . 0 3 ii 3 0 3 3 ee 0 3 3 0 3 0 0 0 0 0 3 0 . 0 - ? M 3. Q a C 0 3 0 - - 3 0 3 0 3 0 a ^ " ? ^dried reddish brown 2 brown / v a*e S&y 9 a3*/1y0 a 236333 .CasaM 8p*ci=y f-- ;si.s Csniwess-ya rds* eiuaosij iie&s?e riaor-*.-esi?9 3iic af eoKr*. 0 0' a a 0 C 0 0 3 0 0 0 0 0 0 0 0 0 0 sag ** --3---- 0 0 0 9 3 3 0 -.-? 23S3S esraea spass.s? f a 2ri. Canjaaesi.va rdjrs ehjBosi^ die&r; riaoE-i-xsrs.?' BtqmiT o; ears** 5 3 0 0 0 0 0 Q 0 0 0 0 * 0 ' any 3 8 3 3 0 C a 9 0 0 Q 0 * 0 CM^ 2363S5 Casa* opACi-ly rft =i8 eaajuaeciva ?da* ehoexij iie&s?c yluoE-l.-t'ssi?3 Blaaie of esss 1 ' 0 9 0 0 0 S 0 0 0 0 9 9 0 0 0 ^ 0 a - 0 Q 0 0 0 e 0 - 0 a9 * ' --sr~ - Wf . a, 2363S6 C5Za opae^sy f-- 2si Csa}ueei7a ;das e&csaclj . iisebkz;* Masr-i-scri?* aisaie af csrsea 9. 0 a 0 0 0 0 5 0 0 3 0 a 0 0 0 0 0 3 0 0 Q 0 0 0 - 0 * B-? " "* -3---- ^ 3*? ""A :33S7 f Cora-- opMisy ;ris e 0 0 Q 0 0 0 Q 0 2 0 0 Canjuac'si-yi fds*a. eiiool die!tr; 3 0 0 0 0 0 S 0 0 3 0 0 ylaes-I.-sss'i?9 - 0 - 0 leoie ef earaaa Mg if ? Cowanysa"-"--"083^co,*""^"' u - en LJ -I Bu m "i i- r o r " n i 0 rI - ill M !{ )! S ^ 01' u > ii' n >i 9 r !rn' (Buf PIi -'PHi M lNr o U M I) li l| r H r r u ; " " 1> u r u i i'() U i* r n I- II s' '"I u ^"^ ^-' u '' '' 01 <r. 1'1 1-1 1J .'! -I 0> S ;' p t? [i1 fl 8 P-S,," 1; (S-ah i o h Sfl&S S8S n !k fi - a- o n p h -- M ii in - w <1 II M M r < ^ n 1| r pit r 11 |i (* N Hi "1 P' 1- u i: " . n i lk ." W&gr2;J{ n i4 n. n ii Uii >1 r K 1 u u t " (i '! n '." " y " n 11 n!-ofiLif' SR8 ii i ui p H C- 1-' U 11 a c u< o n p > n o { i i-' tr -< M 11 P.I: u iiifiuliu 5 0 r1- p(i b U M n 11 * !" i. " !' !i " " a ". n 0 o *( li-au r Nn r "il B8 g P U ('fr.0 W "I"1 1 f tt; '0 t,' K. 8 !' f p^ n 11 E i u h a n>. F[> - ir 5 n n i k u ii I" II M >< M r > jli dl u < 11 >1 .' 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' 0 0 ^j...^ .0. ,,",,",," ," H TABLE III - (0.08 mq/L) H-12,037 ?.K 236339 Cam** ooaex;-/ c ;ri Casluacsiva cda*e eaBOlj discharge ?luor-s.-t=;.?* SicstC oi caw* 23B389 CS?3 op*etsy ?* lrt Cott3uae^i ?&& chaoiJ dj.sehax; yiaor-i-sss:.?* 3iea-e o5 earr-ae 236390 f * SaraM opaeisy Sri Canluae'si.v rdas ehifasis Aics&r5 lBBS-i.-SSS.?9 aicsie ofi easaaa 236391 CaraM afsew uw -S3.S Caaiaaes-ya ;SS;^ &ie!iu? Tiaas-i.-w.?9 3iea-e at assess. 2363S2 essa-- e?acl.w ;* Zf^x Can^ttce^iv ?&r cMnoi Aiea;? y-aos-s.-*^:.?* a-ggis a; ecra 1 reddish brown 5 ia? a/'T4 " 0 0 Q a 0 0 0 0 0 0 Q' 01 0 0 ns ? /^ 3 &iy 8 f 0 0 0 s 0 0 0 0 0 11 1' 0 " sag -- BB'S" 0 0 a 0 0 0 0 0 0 - .- -- 9 0 0 0 asg 0 0 0 0 0 0 0 0 x- s- ^ 0 &? 0 0 0 9 0 0 0 0 0 0 0 0 0 n? 0 0 3 a 0 0 0 0 0 0 0 0 0 B<? g a o a o 0 0 01 - tt"? "I" a 0 0 0 3 0 ; 0 0 Q a 0 0 0 - a? 0 0 3 3 0 0 3 8 1 , ;, - 0 . " s*^ 0 C ; C 3 0 0 C 0 0 r -^ 0 - =; tec SVS^VS 236393 Cars-- optc^ey f Sri. Cocjuaesi-.-a ecda*-- chaaoi. Aiaeluu;* FlKac-l-Kri.?' 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