Document 65qm1yKXabRgjGYYrQneq2evE

A R S 2 6 . 0705 ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE___________________________________________ Identity: Mixture containing perfluorooctanesulfonate which may also be referred to as PFOS, FC-95, or as a component of FC-201F. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 1. The test sample is FC-201 F (F-11129). Current information indicates that FC-201 F is a mixture of 3.0% PFOS, 36.0% diethylene glycol butyl ether, 36.6% water, 8.25% acrylic foamer, 7.0% sodium octyl sulfate, 6.0% sulfonated alkyl ether sodium salts, 3.0% triethanolamine, and 0.15% tolyl triazole. The following summary applies to a mixture with incompletely characterized concentration of impurities. Data may not accurately reflect toxicity of the fluorochemical component o f the test sample. METHOD:___________________________________________________ Method: OECD Guideline 203. Test type: Acute static GLP: No Year Completed: 1992 Species: Pimephales promelas Supplier: ETC, Superior, Wl, maintained at AScl-DETD, Duluth,MN Analytical monitoring: Dissolved oxygen, Temperature, pH, conductivity Exposure period: .96-hours Statistical methods: Not noted Test fish age: Juveniles (45 days) Average Length and weight: 1 L Beakers: Length: Weight: Loading: 1.42 cm 0.034 g Aquarium: Length: Weight: 1.5 cm 0.041 g Beakers: 0.57 g/L 0.34 g/L 0.17 g/L Pretreatment: Not noted. Aquaria: 0.21 g/L 0.12 g/L 0.06 g/L Test conditions: Dilution water: Shallow well water, Two Harbors, MN. Dilution water chemistry: Hardness: 178 mg/L (as CaC03) Alkalinity: 147 mg/L (as CaC03) Lighting: Not noted Stock and test solution preparation: Not noted. Concentrations dosing rate: Once Stability of the test chemical solution: Not noted. Exposure vessels: 1 L beakers having a surface area of 87 cm2 and aquarium having a surface area of 300 cm2. Testing was conducted at several different volumes in each vessel type to study the relationship between the surface area of the exposure chamber to the volume of the dilution water / test solution. Number of replicates: two Number offish per replicate: 5. Number of concentrations: 1 plus a blank control for each vessel type Water chemistry during the study: Conductivity range (0-96 hours): 322 - 391 pmhos/cm (controls) 359 - 400 pmhos/cm (1.0 L exposure in Beakers) 361 - 373 pmhos/cm (3.4 L exposure in Aquariums) pH range (0-96 hours): 8.22 - 8.63 (controls) 8.44 - 8.50 (1.0 L exposure in Beakers) 8.37 - 8.51 (3.4 L exposure in Aquariums) Temperature range (0-96 hours): 20.8 - 21 C (all test vessels) Dissolved oxygen range (0-96 hours): 7.9 - 8.6 mg/L (controls) 8.3 - 8.4 mg/L (1.0 L exposure in Beakers) 8.0 - 8.6 mg/L (3.4 L exposure in Aquariums) RESULTS Type of exposure chamber 1-L Beaker 1-L Beaker 1-L Beaker 1-L Beaker Aquarium Aquarium Aquarium Aquarium D escription o f test system Surface Volum e (L) Volum e (pL) area o f dilution of test (cm 2) water used. substance added 87 1 0 (control) 87 0.3 273 87 0.5 454 87 1 909 300 1 0 (control) 300 1 909 300 1.3 1,545 300 3.4 3,091 Surface area/volum e o f dilution water used 87 290 174 87 300 300 177 88 3h 0 0 0 50 0 0 0 60 % M ortality 6 h 24 h 48 h 72 h 96 h 00000 00000 00000 70 70 70 70 70 00000 00000 20 20 20 20 20 70 70 70 70 70 Element values based on nominal concentrations. 000342 Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The value reported applies to that mixture and not the fluorochemical proportion alone. CONCLUSIONS______________________________________________ No reliable conclusions can be made based on this study. However, the data indicates that the acute toxicity of the test sample may be dependent upon the ratio of the surface area of the exposure vessel to the volume of the test solution. The test sample appeared to be acutely toxic only when the surface area to volume ratio was about 87-88 cm2/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________________ Reliability: Klimisch ranking 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. REFERENCES__________________________________________ ;___ _ This study was conducted by AScI Corporation, Duluth Environmental Testing Division, Duluth, MN at the request of the 3M Company, Lab Request number K1792, 1992. OTHER________ ,____________________________________________ Last changed: 6/26/00 000343 ASci CorpofUioo/AScI-Dululii AESncvIirRoenpmoretnDtalJ#T0eIt5ta-FHDAiv.Ris3ioMn AScI Study ID# S030-OI5-03 STUDY TITLE FURTHER INVESTIGATION ON THE ACUTE TOXICITY OF FC-201F TO FATHEAD MINNOW (PIMEPHALES PROMELAS) DATA STANDARD OECD GUIDELINE 203 STUDY COMPLETED October 5, 1992 TESTING FACILITY AScI Corporation AScI-Duluth Environmental Testing Division 112 East Second Street Duluth, MN 55805 Tel. No. (218) 722-4040 STUDY IDENTIFICATION unit; ERS AScI Study ID# 5030-015-03 3M Company Lab Request# K1792 Notes: This report is a continuation of the AScI Report ID# 015-FHA.R3M for AScI Study IDf 5030-015-03. The original study was completed on August 24, 1992. SSppoonnssoorrS3tuMdyCOEom>#paKn1y792 11 000344 EAnSvicrIoCnmorepnotarlatTioens/tAinSgcDI-Divuisluioni AScI Report ID# 015-FHA.R3M AScI Study ID# 5Q30-01J-03 1.0 GENERAL INFORMATION Study Title Data Standard Sponsor Sponsor's Representat ive Sponsor's Lab Request# Testing Facility Principal Investigator Project Director Testing Facility Director Test Dates Data Validation and Report Review Retention of Raw Data and Final Report Location of Raw Data and Final Report Further Investigation on the Acute Toxicity of FC-201F to Fathead Minnow (Pimephales promelas) OECD Guideline 203. 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106. Susan A. Beach, 3M Environmental Laboratory, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55106; Tel No. (612) 778-7452. K1792. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55-805; Tel. No. (218) 722-4040. Jo A. Thompson Dinesh Vaishnav Donald Mount August 28-September 1, 1992. Dinesh Vaishnav Report Signature: /o/r/Vi -- - ) TJ Two years from the date study is completed. AScI Corporation/AScI-Duluth Environmental Testing Division, 112 East Second Street, Duluth, MN 55805; Tel. No. (218) 722-4040. Sponsor 3M Company Sponsor Study ID# K1792 12 000345 AESAneASvlicSrRIocenCIpmSootreruptndoTtysnO!tT#iTcO0oe#1/dA55in-0SFe3cH0ID--AD0iv.1Rui5sl3-iuo0Mtn3h 2.0 OBJECTIVE(S) AND RESULTS To determine the effect of the surface area to volume ratio on the acute toxicity of the test substance, FC-201F, to fathead minnow (Pimephales promelas) under static test conditions. This study was conducted according to the AScI study plan retained by the Sponsor, OECD Guideline 203 (OECD 1984), and the previous report submitted for this particular test substance. Results from this study are described in Table 1.______________ 3.0 CONCLUSIONS 1. The test substance acute toxicity appears to be dependent upon the ratio of the surface area of the exposure chamber to the volume of the dilution water, but not on the type of the exposure chamber. 2. The test substance was acutely toxic only when the above ratio was about 87-88 cm-square L'1. 4.0 REFERENCE Organization for Economic Cooperation and Development (OECD). 1981/1984. OECD Guidelines for Testing of Chemicals. OECD Publication Information Center, Washington, D.C. Sponsor 3M Company Sponsor Study TD # K1792 13 000346 EAnSvicrioCnmaiepnotnalliTooc/sAtinSfcID-Diviidsiuolnh AScI Report ID 015-FHA.R3M AScl Study ID SOKMUS-OS Table 1. Acute toxicity of 1,000 mg/L FC-201F (test substance) to fathead minnows (P . promelas) in various test systems Description of test system Type of exposure chamber Surface Volume (L) area (cm- of dilution square) water used 1-L Beaker 1-L Beaker 1-L Beaker 1-L Beaker Aquarium Aquarium Aquarium Aquarium 87 87 87 87 300 300 300 300 1 0.3 0.5 1 1 1 1.3 3.4 Volume (pi) of test substance added 0 (control) 273 454 909 0 (control) 909 1,545 3,091 Surface area/volume of dilution water used 87 290 174 87 300 300 177 88 % Mortalityr 3 h 6 h 24 h 48 h 72 h 96 h 000 0 0 0 000 0 0 0 000 0 0 0 50 70 70 70 70 70 000 0 0 0 000 0 0 0 0 20 20 20 20 20 60 70 70 70 70 70 *^ I M Sponsor. 3M Company Sponsor Study ID K1792 14