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5::.; 5: " n: PPIILLOOTT RRAATT TTEERRAATTOOLLOOGGYY SSTTUUDDYY 3 TT--33335522 { :::::: .FFIIN. NAALL RREEPPOOR. RTT . / .:: : HAZLETON m LABORATORIES AMERICA, INC. LABORATORIES AMERICA, INC. 9200 LEESBURG TURNPIKE, VIENNA,VIRGINIA 52180, U.S.A. -::: a ae ~'HONI~ (~'03] B93 5400, TELEX 899436 (HAZLABS VINA), CABLE HAZ/ABS WASH DO Exhibit 2786 22778866..00000011 cone avo 33MMAA0000224488228866 1I 1I @uazeiemoume 1I I 1 I 1 PPIILLOOTT RRAATT TTEERRAATTOOLLOOG6YY SSTTUUDDYY I1 TT--33335522 II11 FFIINNAALL RREEPPOORRTT I 1 1I 1I 1I 1I 1I SSuubbmmitittteedd ttoo 1I 3MM Commppaany 1I Stt.. PPaauull,, MMiinnnneessoottaa I1I11I MWaayy 1111,, 11998833 22778866..00000022 m3MA0A002024408226877 I 1I @HAZLETON| I SIRRCT: prio fa Teratology tats SUBJECT: Pilot Rat Teratology Study Hal Project No. 154-159 Ii Ve, the designed, hereby dslare tat the ork wis pertrend We, the undersigned, hereby declare that the work was performed I nt oS nas Ne str under our supervision, according to the procedures herein described. Sta ptectars I Study Director: I Lit Tierr 1 S[SLttAaaWffRffENSSCEcciiTee.nn-tttii;IssfttjTZEL, Ph.D. Soar Toxtantony Department of Toxicology I1I sores pes ---- Laboratory Supervision: Teratology: I Gaff 1 Rodent Toxicooay 1 GEORGECA. BURDOCK, Ph.D. Rodent Toxicology I I a 5 tatony Department of Toxicology Bont Duelo RRRUeeTssHeeaarrScc.hhDAUAsRssLsoOoc0ci,iaattBee.S. Sereno Toreatony Department of Toxicology I I II 1 1I on blm I Separt raparation: Report Preparation: Mpadeong e. MARCIA A. MENSE, B.A. TSeiEl iieorices vparoant Technical Writer Scientific Resources Department I I I 22778866..00000033 swsorziasa 3MA00248288 I I @HAZLETONucomrons svenca ne I I rae 1 TTAABBLLEE OOFF CCOONNTTEENNTTSS Page I rRONUCTION 3 1 SSuUMmMAyRY i 1 INTRODUCTION 1 CCOONNTTRROOLL AANNDD TTEESSTT MMAATTEERRIIAALLSS 3 I1 TEST Avs ` TEST ANIMALS I WEH0DS . METHODS 4 Hating Period 3 1 GMGrraootuuinppsgs PaanneddrioDDdoossaaggee LLeevveellss 54 5 Conpoing reparation and Adninistration : Compound Preparation and Administration 6 I Haternai Observations and Records s Maternal Observations and Records Cesarean Sacrifice and Tissue Preservation 7 Cesarean Sacrifice and Tissue Preservation 7 1 Statistical Analyses : Statisti cal Analyses 8 l Spacinen, Rau Data, and Firal Report Storage s Specimen, Raw Data, and Final Report Storage 9 1 RESULTS - MATERML DATA 10 RESULTS - MATERNAL DATA i0 Crinica1 Signs i Clinical Signs i0 I Hortaiity 10 Mortal ity BBooddyy W Weeiigghhttss i0 I100 I1 Foot Consumption 1 GFGrorooosdsss CPPoanatsthhuoomllopogtgioyyn Ii1Ii UUtteerriinnee WWeeii gghhttss uini 1I Regus csi pn 2 RESPPFUerretSLeaTagngaS]dnnaanVn-ITmcicoyaypCpbElmRiRSaalAanatiRtttetEeaisyAst,,Nio,onHnCCDeoAoiErTErgAppfhtooftirir,acaciieeLLannunucctdteeyyaaS,e,xUUtteerriinnee plantations, Implantations, Ix12 12 I FFeettaall Deveroment Viability, Weight, and Sex i13 13 Fetal Devel opment !1 aus TATBTTaLaaEbbbSllleee 211 --- HCCollaiinnniiccMaaaltl eOrOnbbassleerrvovaadttiyioonnesstaDDhuutrrsiinnggandGGeesSstotadatytiiooHnneight Change 1115s5 i 1 TTaabbllee TTTaaabbbllleee Table 452 3 3 4 ---- - MMMeeeaaannn MFFcoaootdderCnCoaonlnssuBumompdptytiiooWnneiVVgaahlltsuueessand Body Weight MSeuamnarTyermoifnaGlrosBsodpyaNtheoilgohgty,sFGinrdaivnigdsUterine Su,~mary of Gross Pathology Findings Change Weights, and 111u67 18 Tabl e 5 - MeTaanorTmeinrma)inaSol dyBoHdey1gWheisighMtisn, usGrGarvaidvidUtUetreinreinW e eAicghttos,s and 2200 I1 Koy to Tate's 3 TKTTaaaebbybilleeteo's67Ta-= bhSSleeuuaTnmn~6eanrramTryyhincooaiflfenOOBcvoveadaryrfViaaW anlne,l,iegsUUhtttseferorirMinnieenV,ui,sscaaoGnnrddraalLLviiidttattneeUdrrtSekDDreaianltteeaataWleiFgihntsdings 2222123 I 1 Table 7 - Mean Incidence KKeeyy ttoo TTaabbllee 88 Tablas ~ MeapnerInLciitdteenrce Table 8 - Mean Incidence of Visceral and Skeletal Findings Values for Visceral and Skeletal Findings of Visceral and Skeletal Findings =23 2255 per Litter 26 I I 22778866..00000044 swaonzsezss 3MA00248289 I I 1 @HAZLETON ueonsronesmemcane LABORATORIES _AMERICA, . I TTAABBLLEE OOFF CCOONNTTEENNTTSS ((CCOONNTTIINNUUEEDD)) 1 pagePave I APPENDICES APPENDICES 1 Key to Aependix 1 7 Key to Appendix i 27 I Aependta 1 Individual end Mean Maternal Body Weights and Appendix I - Individual and Mean Maternal Body Weights and eign Changes = Weight Changes 28 1 appendix 2 = Individual ang Mean Daily and Total Food Consumption 31 Appendix 2 - Individual and Mean Daily and Total Food Consumption 31 Appendix 3 Individual Body Nelgnts, Gravid Uterine Neignts, and Appendix 3 - Individual Body Weights, Gravid Uterine Weights, and I Terminal Body el his Minus Gravid Utering Weights 34 Terminal Body Weights Minus Gravid Uterine Weights AAppppeennddiixx 44 -- IInnddiivviidduuaall aanndd MMeeaann OOvvaarriiaann,, UUtteerriinnee,, aanndd LLiitttteerr DDaattaa 3374 37 Ii Ropencix 5 - Individual and Mean Live Feta Data i 1 Appendix 8 - References 3 Appendix AAppppeennddiixx 5 66 - - - Individual IInnddiivviidduuaall and Mean VViisscceerraall FFLiiivnneddiinnFggesstaffloorrDaEEtaaacchh LLiitttteerr 40 4433 AAppppeennddiixx 77 -- IInnddiivviidduuaall SSkkeelleettaall FFiinnddiinnggss ffoorr EEaacchh LLiitttteerr 444a i Appendix 8 - References 46 I, I I I I I I i I 22778866..00000055 aMADO248290 3MA00248290 1 EYHAZLETON uiorurones senca nc LABORATORIES AMERICA, INC. 1 SSPPOONNSSOORR:: 33M CCoommppaannyy I MATERIAL: T-3352 DDAATTEE:: tMaayy 1111,, 11998633 1 Pi $S1UJBBJJEECCTT:: FFIINNAALL RREEPPOORRTT PPiilloott RRaatt TTeerraattoollooggyy SSttuuddyy Project No. 154-159 } sonar SUMMARY 1 sn, sh 41 5. pg Ss T-3352, suspended in corn oil at concentrations intended to I ddeelliivveerr I1,, 1100,, 3377..55,, oorr 7755 mmgg//kkgg//ddaayy ((GGrroouuppss 22,, 33,, 44,, aanndd 55,, 1 rreessppeeccttiivveellyy)),, wwaass aaddmmiinniisstteerreedd bbyy oorraall iinnttuubbaattiioonn ttoo ffoouurr ggrroouuppss ooff I mmaatteedd ffeemmaallee SSpprraagguuee--DDaawwlleeyy SS--DD rraattss oonn ggeessttaattiioonn DDaayyss 66 tthhrroouugghh 1155.. AA ffiifftthh ggrroouupp ((GGrroouupp 11)) ooff mmaatteedd ffeemmaalleess sseerrvveedd aass tthhee ccoonnttrrooll aanndd rreecceeiivveedd 1 tthhee vveehhiiccllee oonnllyy.. I iran tot tres of oon rom tt on ox te ow 1 Apparent ccoommppoouunndd--rreellaatteedd ddeeaatthhss ooccccuurrrreedd iinn sseevveenn ooff sseevveenn Group 5 and three of seven Group 4 females on or before Day 17 of gestation. Clinical observations in Groups 4 and 5 included hunched, thin appearance, languid behavior, and apparent anorexia. Body weight 1 ggaaiinn dduurriinngg ttrreeaattmmeenntt wwaass lleessss iinn GGrroouupp 33,, 44,, aanndd 5 ffeemmaalleess ccoommppaarreedd ttoo control values obtained during that time. The terminal body weight minus 1 tn ets aterm wiih vey ORE S08 4 Sih SEES 0 the gravid uterine weight was decreased in Group 4 females compared to I control. A number of gross pathology findings at necropsy or sacrifice 1 sre tad fn Groups od 5 on tase clad nonce of Ther, were noted in Groups 4 and 5 and these included incidences of liver, adrenal, lung, and/or gastrointestinal tract effects. 22778866..00000066 3MA00248291 I 1 ! 1 @HAZLETONueorronesmma ne ~ H LA~Z'~~r~ LABORATORIES _AMERICA, INC. ! -z- 1 115544--115599 -2- I1 Elation of nen fetal weight data indicated that feta] Evaluation of mean fetal weight data indicated that fetal I1 Wits of both sexes decressed with fncreasing dose level. A i wseiiggnhitfsicanotf debcortehasesefxnesmeadnecrfeetaasled weiwgihtth ocicnucrrreeadsinign Gdroosuep 4lemvaelle.s andA significant decrease in mean fetal weight occurred in Croup 4 males and ! females, although data for only tio litters were available for that 1 females, although data for only group. Cleft. palate was fond in two two olfitstiexrs Grwoeurpe 4 afveatiulsaebsle (ofnoer ftehtauts group. Cleft palate was found in two of six Group 4 Fetuses (one fetus ! iinn eeaacchh lliitttteerr)) eexxaammiinneedd bbyy WWiillssoonn''ss tteecchhnniiqquuee.. IInnccoommpplleetteellyy ddeesscceennddeedd 1 estes vere oted in ane fetus fram cach Group 4 litter evaluated for testes were noted in one fetus from each Group 4 litter evaluated for I Visceral defects. Incidences of skeletal varfants were similar for al visceral defects. Incidences of skeletal variants were similar for all 1I ggrroouuppss.. 1I 1I i 1 1I I 1 1I 1i I 1 1I 1I 22778866..00000077 amaonzsszs 3MA00248292 I 1 ! I HAZLETON vscnronessemen ic. 1 Pete unmeonae ots -3- 154-159 !I1 rection INTRODUCTION I1 This study was designed to determine the maternal and. anbryo/ ffeettaall This study was designed to determine the maternal and embryo/ ttooxxiicciittyy ooff TT--33335522 wwhheenn aaddmmininiisstteerreedd bbyy ggaavvaaggee ttoo pprreeggnnaanntt rraattss I during the period of fetal organogenesis for the purpose of setting dose 1 levels for an expanded teratology stuly. The rats were placed in levels for an expanded teratology study. The rats were placed in ! breeding on January 17, 1983, and cesarean sections were completed on breeding on January 17, 1983, and cesarean sections were completed on 1 February 18, 1983. This report presents the methods and results from February 18~ 1983. This report presents the methods and results from I this study. this study. !11 CCOONNTTRROOLL AANNDD TTEESSTT MMAATTEERRIIAALLSS I The vehicle and control material, Duke's Corn OF1 (C. F. Saver The vehicle and control material, Duke's Corn Oil (C. F. Sauer 1 CCoo..,, RRiicchhmmoonndd,, VViirrggiinniiaa)),, lloott 8800223355,, wwaass rreecceeiivveedd oonn JJaannuuaarryy 2244,, 11998833,, I1 and vas stored at roo temerature. and was stored at room temperature. i The test material, T-3352, an off-white solid, was received from The test material, T-3352, an off-white solid, was received from I test material was assumed to be 1004 active compound. Information on 1 tthhee ssppoonnssoorr oonn JJaannuuaarryy 55,, 11998833,, aanndd wwaass ssttoorreedd aatt rroooomm tteemmppeerraattuurree.. TThhee test material was assumed to be 100% active compound. Information on I1 methods of synthess and stability, as well as data on composition or other characteristics hich define the test material, are on file with methods of synthesis and stability, as well as data on composition or other characteristics which define the test material, are on file with 1I sr sr the sponsor. 1! I1, iI 1i 22778866..00000088 aMA00240293 3MA00248293 I 1 ! 1 @HAZLETON sonrones auemca ne .I1 mmm-------- "e -4- 115544--115599 I I TTEESSTT AANNIIMMAALLSS 1 SSeexxuuaallllyy mmaattuurree cceessaarreeaann--ddeerrivveedd SSoprraagguuee--DDaavwlleeyy CCrrlI::CcDo ((SSDD))SBRR ! rraattss wweerree cchhoosseenn ffoorr tthhiiss ssttuuddyy bbeeccaauussee tthheeyy aarree sseennssiittiivvee to to a a nurber number of of !1 srt hic re ace 15% mg. gor sez. oo agents which are known to be embryotoxic and/or teratogenic. Rats have ! required by the regulatory agencies. Thirty-six tuelve to fourteen-neek 1 hhiissttoorriiccaallllyy bbeeeenn uusseedd iinn ssaaffeettyy eevvaalluuaattiioonn ssttuuddiieess ooff tthhiiss ttyyppee aanndd aarree required by the regulatory agencies. Thirty-six twelve to fourteen-week 1 O16 male rats and thirty-six eight to tanwesk ola female rats were old male rats and thirty-six eight to ten-week old female rats were ! received fron Charles River Breeding Laboratories, Kingston, New York, on received from Charles River Breeding Laboratories, Kingston, ~ew York, on 1 star 2, 1502. Th ras wre eld in curative for thr and December 22, 1982. The rats were held in quarantine for three and ! oonnee--hhaallff wweeeekkss,, dduurriinngg wwhhiicchh ttiimnee a a health health status status examination examination was was I 1 ppeerrffoorrmmeedd bbyy aa ssttaaffff vveetteerriinnaarriiaann.. 1 The rats vere haused one male and one female per cage during The rats were housed one male and one female per cage during ! bbrreeeeddiinngg.. FFoolllloowwiinngg ccoonnffiirrmmaattiioonn of of meting, mating, the the females females were were housed housed i iinnddiivviidduuaallllyy iinn eelleevvaatteedd wwiirree--mneesshh ccaaggeess wwiitthh ffoooodd ((PPuurriinnae RRooddeenntt ! LLaabboorraattoorryy CChhooww~ 55000011)) aanndd ttaapp wwaatteerr aavvaaiillaabbllee aadd lliibbiittuumm.. TThhee ffeemmaalleess 1! ae nicely dun1ed by ar tap ater cating was confirm. Tamera: 1 were uniquely tures in the identified study room by ear ranged tag after mating was confirmed. from 70 to 78F with a relative Theummpiedriat-y tures in the study room ranged from 70 to 78F with a relative humidity ! between 44 and 691. between 44 and 69%. !1 WETHODS METHODS 1! Mating perio Mating Period 1 during the mating period, one fesale sas paired with one wale During the mating period, one female was paired with one male 1!!!1 uunnttiill mmaattiinngg wwaass ccoonnffiirrmmeedd oorr uunnttiill ttwwoo wweeeekkss hhaadd eellaappsseedd.. Daily vaginal Daily vaginal 22778866..00000099 aMADO248204 3MA00248294 I I 1 E{~@YHe HAA~Z,LL,IE~'r TI:IO~Nr~vLoAnBOe oRAnTOeRsIES AeMEoRInCA,nINeC.. se I 5- ots 154-159 ! examinations of each female were performed to detect the presence and 1 vviiaabbililiittyy ooff ssppeerrmm oorr tthhee pprreesseennccee ooff aa ccooppuullaattoorryy pplluugg.. TThhee ddaayy ooff ! observation of spera or copulatory plug vas designated as "Day 0 of observation of sperm or copulatory plug was designated as "Day 0" of I1 gestation. gestation. 1 GGrroouuPpss aanndd. DDoossaaggee LLeevveellss I Upon confirmation of mating, each female was assigned to one of Upon confirmation of mating, each female was assigned to one of i the following groups. the following groups. I GGrroou;up DDoossee"a NNuummbbeerr ooff FFeemmaalleess 1 fe wore fuser fei mglkglday I 11 00 1 2 1 6 s & 2 3 101 76b 1 3 a a10 s 77 7 4 37.5 I 55 77%5 77 1 during the dosing period. a2BBaasseed on individual animal body weeiigghhttss aatt eeaacchh wweeiigghhiinngg iinntteerrvvaall during the dosing period. I1 5 hn adttanal Group 2 female was confirmed to have mated on a day shen no 1 b AoGRnativheearrddbiemmteaanottininaknglngsos.wGnrwwoeeuarrptee c2cceoofsnneaffmriiasrrlammneeeddws..aascricBfoSeinecccfaaeiuu,rssmeeesdhttehhtiiowssash.aaannvriieemnnaaomllva''etssdedfddrooonossneeatllhdeeeavvyeesllwthuedwwynoo.uullnddo have been known at cesarean sacrifice, she was removed from the study. i The females were placed into the dose groups one at @ tine The females were placed into the dose groups one at a time 1I begiming with the high-dose group and continuing sequentially through 1 beginning with the high-dose group and the control group until all mated females were assigned to group. continuing sequentially through the control group until all mated females AL mates vere sacrificed via cwoerrdeonasdsiigonxeidde toasaphgyrxoiuapt.ion end i All males were sacrificed via carbon dioxide asphyxiation and 1 discarded without necropsy once a sufficient musber of matings were discarded without necropsy once a sufficient number of matings were / confirmed. confirmed. I I 22778866..00001100 aMAD0248295 3MA00248295 1 1I HAC ZLETONwomy sronss erica.ne. 6 - ots 154-159 I RPP Compound Preparation and Administration prepared on a weignt per volume basis. The required smut of compound 1 SSuussppeennssiioonnss ooff tthhee tteesstt mmaateterriiaall iinn tthhee ccoorrnn ooiill vveehhiiccllee wweerree prepared on a weight per volume basis. The required amount of compound 1 for Tews 2, 3, or wis weighed nto a homgenizer on an electronic arbor 126 balance. Compound for Group 5 wes weighed on poder paper on for levels 2, 3, or 4 was weighed into a homogenizer on an electronic Arbor 126 balance. Compound for Group 5 was weighed on powder paper on 1 an trbor 126 balance and vised into a Haring Slender. pproxiastely & am Arbor 126 balance and rinsed into a Waring blender. Approximately 4 1 a1 of com ofl was added and the test material was ground into 3 fine ml of corn oil was added and the test material was ground into a fine I! suspension. This suspension was rinsed fron the homogenizer or the suspension. This suspension was rinsed from the homogenizer or the 1 bblleennddeerr iinnttoo aa bbeeaakkeerr aanndd aaddddiittiioonnaall ccoorrnn ooiill wwaass aaddddeedd ttoo pprroodduuccee tthhee desired concentration. The sussension was mixed on a stirrer for desired concentration. The suspension was mixed on a stirrer for 1 approcinately fifteen minutes. Prepared suspensions were mixed with 3 approximately fifteen minutes. Prepared suspensions were mixed with a mugretic stirrer during dosing. magnetic stirrer during dosing. Females were given the appropriate dosing suspension or vehicle 1 by oral intubation on a daily basis begining on Day 6 and continuing by oral intubation on a daily basis beginning on Day 6 and continuing through Day 16 of gestation. The test material was administered orally through Day 15 of gestation. The test material was administered orally 1 because of th relative ese and accuracy of dosing. Fresh dosing suspensions were prepared weekly and stored under because of the relative ease and accuracy of dosing. Fresh dosing suspensions were prepared weekly and stored under 1 refrigeration (proximately 4%). Swmples of sich test mixture as 1 well ss the vehicle were sent to the sponsor for analysis. refrigeration (approximately 41F). Samples of each test mixture as well as the vehicle were sent to the sponsor for analysis. Vaternal Observations end Records Maternal Observations and Records All animals were examined twice daily for mortality and mori- 1 bundity (fron day of recefpt through study termination) and once caily bundity (from day of receipt through study termination) and once daily for clinical signs of toxicity and pharmacologic effects (throughout for clinical signs of toxicity and pharmacologic effects (throughout 1 ,1! 22778866..00001111 amonziszss 3MA00248296 1 11 HS AZLETONuLsAoBa OmRrAoTOnRsIEsS sAeMnERiICA, nINeC. ress 154-159 ! ashton). iy wih sd fe cansetion ar record on 7, 1 6.8, 12, 16, and 20 of gestation. gestation). Body weights and food consumption were recorded on Days O, 6, 8, 12, 16, and 20 of gestation. I 1 Cesarean Sacrifice and Tissue Preservation Cesarean Sacrifice and Tissue Preservation I AA ggrroossss nneeccrrooppssyy wwaass ppeerrffoorrmmeedd oonn aallll aanniimmaallss ((ffoouunndd ddeeaaddss,, 1 moribund sacrifices, and terminal sacrifices). The uterus and ovaries moribund sacrifices, and terminal sacrifices). The uterus and ovaries i fron found dead animals and animals sacrificed becasse of moribundity from found dead animals and animals sacrificed because of moribundity 1 were removed and examined for the mmber of implantation sites and were removed and examined for the number of implantation sites and I corpora utes, respectively. Prior fo gestation Day 2, surviving corpora lutea, respectively. Prior to gestation Day 20, surviving I females were assigned random numbers and all personnel performing 1 cesarean sections and/or external, visceral, or skeletal examinations of cesarean sections and/or external, visceral, or skeletal examinations of I the fetuses vere unaware of the dose level fron wnich the antasls vere 1 derived. On Day 20 of gestation, all surviving females were weighed and the fetuses were unaware of the dose level from which the animals were derived. On Day 20 of gestation, all surviving females were weighed and I sacrificed by carbon dioxide asphyxiation. sacrificed by carbon dioxide asphyxiation. 1 The uterus fron escn fensle as veighed and exomied for the The uterus from each female was weighed and examined for the I nnuummbbeerr aanndd ppllaacceemmeenntt ooff uutteerriinnee iimmppllaannttaattiioonn ssiitteess,, nnuummbbeerr ooff lliivvee aanndd ! dead fetuses, early and late resorbing fetuses, and any abnormalities. I The ovaries were examined for the number of corpora lutea. Each live |} TThhee uutteerruuss ooff eeaacchh aanniimmaall wwaass rreewweeiigghheedd aafftteerr tthhee ccoonntteennttss wweerree rreemmoovveedd.. The ovaries were examined for the number of corpora lutea. Each live 1I fetus vos sexed, weighed, and exsmined for external abnormalities. Findings were recorded. fetus was sexed, weighed, and examined for external abnormalities. Findings were recorded. 1 ego 35 the evrin rd of he rig uterine orn, te Beginning at the ovarian end of the right uterine horn, the i1 first sic fetuses, regadiess of sex, were selected for further first six fetuses, regardless of sex, were selected for further I11II evaliation. After the external examination was completed, the first, evaluation. After the external examination was completed, the first, 22778866..00001122 swao02eezs7 3MA00248297 1 1 e. 1 @HAZLETONuaonsronessenca nc. LABORATORIES AMERICA, INC. rmmm------ -8- 115544--115599 I 1 nr od 1 fetus rs. red ih 1a ad i 1 ove third, and fifth fetuses were identified with a tag and fixed in Bouin's ! Fourth, and sixth fetuses were eviscerated, tagged, and processed for 1 ssoolluuttiioonn ffoorr ssoofftt ttiissssuuee eevvaalluuaattiioonn ((WWiillssoonn,, eett aall,, 11996655)).. TThhee sseeccoonndd,, fourth, and sixth fetuses were eviscerated, tagged, and processed for I 1 sskkeelleettaall eexxaammiinnaattiioonn uussiinngg aa tteecchhnniiqquuee mmooddiiffiieedd ffrroomn tthhaatt rreeppoorrtteedd bbyy SSttaapplleess ((SSttaapplleess aanndd SScchhnneellll,, 11996644)).. I 1 SSttaattiissttiicacla.l.AA~.naall.yysseess 1 MMeeaann mmaatteerrnnaall bbooddyy wweeiigghhtt gcaaiinnss ((DDaayyss 66--1166 aanndd 00--2200)),, ttoottaall ffoooodd I ccoonnssuummppttiioonn,, ggrraavviidd uutteerriinnee wweeiigghhtt,, tteerrmmiinnaall bbooddyy wweeiigghhtt mmiinnuuss ggrraavviidd 1 erie vet, reoration fndece, pecan mates nd fetal vihiTity uterine weight, resorption incidence, percent males, and fetal viability I 1 wweerree aannaallyyzzeedd iinn tthhee ffoolllloowwiinngg oorrddeerr.. LLeevveennee''ss tteesstt ffoorr hhoommooggeenneeiittyy ooff I vvaarriiaanncceess ((LLeevveennee,, 11996600;; ODrraappeerr aanndd HHuunntteerr,, 11996699)) wwaass ppeerrffoorrmmeedd aanndd iiff 1 tthhee vvaarriiaanncceess pprroovveedd ttoo bbee hhoommooggeenneeoouuss,, tthhee ddaattaa wweerree aannaallyyzzeedd bbyy oonnee--wwaayy I ccllaassssiiffiiccaattiioonn aannaallyyssiiss ooff vvaarriiaannccee ((AANNOOVVAA)) ((WWiirnieerr,, 11997711)).. IIff tthhee I variances proved to be heterogeneous, a series of transformations was variances proved to be heterogeneous, a series of transformations was I ppeerrffoorrmmeedd uunnttiill vvaarriiaannccee hhoommooggeenneeiittyy vwaass aacchhiieevveedd.. TThheessee ttrraannssffoorrmmaattiioonnss I 1 wweerree:: lloogglyo,, ssqquuaarree ((xX22)),, ssqauarree rroooott ((Xx~9),, rreecciipprrooccaall ((1l7/X%)),, 1 aanngguullaarr ((aarrccssiinnee XX)),, aanndd rraannkk,, finn tthhaatt oorrddeerr.. IIff rraannkk ttrraannssffoorrmmaattiioonn I wwaass. iinneeffffeeccttiivvee iinn rreemmoovviinngg vvaarriiaannccee hheetteerrooggeenneeiittyy,, AANNOOVVAA ooff rraannkkeedd ddaattaa 1 was comets. If MWA of wtrantored or trinsforsed ta vas was completed. If ANOVA of untransformed or transformed data was I ssiiggnniiffiiccaanntt,, ODuunmneetttt''ss tt--tteesstt ((DDuunmneetttt,, 11995555 aanndd 11996644)) wwaass uusseedd ffoorr 1 Control vs. compndetrested ro sen compra. If HOW was oot control vs. compound-treated group mean comparisons. If ANOVA was not 1!I1!I1 ssiiggnniiffiiccaanntt,, tthhee aannaallyyssiiss wwaass ccoommpplleettee.. 22778866..00001133 a3MMAA000022448229988 1I I 1 @ !~HHA~ZL.~ ETrO~ NwLsAoenOa.ArToOnR,eEsSmAeMEs.,oCsA. n,NeC.. m---- 1I 9 - 115544--115599 I 1 In aketion to the sb deta, amiss of covriame (COW) In addition to the above data, analysis of covariance (ANCOVA) 1 ((WWiinneerr,, 11997711)) wwaass uusseedd ttoo aannaallyyzzee mmeeaann ffeettaall wweeiigghhttss.. TThhee lliitttteerr wwaass I used as the experimental unit. used as the experimental unit. 1 Levene's tests and ANOVA were evaluated at the 5.06 one-tailed Levene's tests and ANOVA were evaluated at the 5.0% one-tailed I pprroobbaabbiilliittyy lleevveell.. CCoonnttrrooll vvss.. ccoommpoouunndd--ttrreeaatteedd ggrroouupp mmeeaann ccoommppaarriissoonnss 1 ere evaluated at the .0 to-taiTed pranabiliy level. were evaluated at the 5.0% two-tailed probability level. ! Statistical references are appended to this report, and statisti- Statistical references are appended to this report, and statisti- I cally significant differences, as indicated by the aforementioned tests, 1 aarree ddeessiiggnnaatteedd tthhrroouugghhoouutt tthhiiss rreeppoorrtt bbyy tthhee tteerrnm ""ssiiggnniiffiiccaanntt"? aanndd//oorr aass I ffoolnlaowwss:: I 1 5S++ == SSiiggnniiffiiccaannttllyy hhiigghheerr tthhaann tthhee ccoonnttrrooll vvaalluuee.. 1 5- = Significantly lover than the control value. S- = Significantly lower than the control value. I Speciren, Ra Data, aid Fina) Report Storage Speci.men, Raw Data, a6d Final Report Storage 1 AAlIl ssppeecciimneennss,, rraaww ddaattaa,, aanndd tthhee ffiinnaall rreeppoorrtt aarree ssttoorreedd iinn, tthhee I 1 archives of Hazleton Laboratories Anerica, Inc. I archives of Hazleton Laboratories America, Inc. I 1 ! 1 1 1 1I 1I 1I 22778866..00001144 aMAD024829 3MA00248299 I 1 I1 @HAZLETON usomrories mena ve. 1 mm---- S10- - 10 - !1 ners - wr, or ! RESULTS - MATERNAL DATA 115544--115599 1 Simic sans Clinical Signs. ! 1 SSuummmaarryy cclliinniiccaall ssiiggnnss aarree pprreesseenntteedd iinn Table Table 1. 1. ! TTrreeaattmneenntt--rreellaatteedd cclliinniiccaall oobbsseerrvvaattiioonnss were were noted noted In in the the Groups Groups 1 44 aanndd 55 aanniimmaallss dduurriinngg ttrreeaattmmeenntt aanndd iinn GGrroouupp 44 aanniimmaallss posttreatment. posttreatment. I No Group rats survived the treatment phase. Clinical signs for Groups No Group 5 rats survived the treatment phase. Clinical signs for Groups 1 4 and 5 antaals focluded hunched and thin appearance, Tonguid behavior, 4 and 5 animals included hunched and thin appearance, languid behavior, I uurriinnee ssttaaiinnss,, aanndd bblloooaddyy ccrruusstteedd eyes, eyes, eyelids, eyelids, rose, nose, mouth, mouth, legs, legs, paws, paws, I or genitals. Anorexia, ataxia, dyspnea, rough coat and pale appearance 1 Vers 2150 noted for several Group & or 5 rats. One Grow 4 rat aborted were also noted for several Group 4 or 5 rats. One Group 4 rat aborted ! tthhiirrtteeeenn ffeettuusseess oonn DDaayy 2200 pprriioorr to to sacrifice. sacrifice. Alopecia was noted for Alopecia was noted for ! 1 Ssoommee rraattss iinn eeaacchh ddoossee ggrroouupp aanndd tthhee ccoonnttrrooll ggrroouwp.. 1 MoMorrttaalliittyy ! AAllll GGrroouupp 5 5 ffeemmaalleess wweerree ffoouunndd dead dead on on or or before before Day Day 16 16 of of 1 ggeessttaattiioonn aanndd tthhrreeee GGrroouupp 44 ffeemmaalleess were were found found dead dead on on or or before before Day Day 17 17 ! of gestation. All other animals survived to cesarean sacrifice on Day 20. of gestation. All other animals survived to cesarean sacrifice on Day 20. I1 BBooddyy VWeeitgghnttss 1 IInnddiivviidduuaall aanndd mmeeaann body body weights weights and and body body weight weight changes changes are are ! pprreesseenntteedd iinn AAppppeennddiixx 11.. Mean weight values and body weight changes are Mean weight values and body weight changes are 1 presented in Tt 2. presented in Table 2. ! 1 DDuurriinngg ggeessttaattiioonn,, lloowweerr than than control control mean mean body body weight weight values values ! Wweerree nnootteedd iinn GGrroouupp 5 5 bbeaggiinmniinngg on on Day Day 8 8 and and in in Group Group 4 4 beginning beginning on on Day Day !I11 1122.. DDeeccrreeaasseedd bbooddyy wweeiigghhtt ggaaiinn during during gestation gestation was was noted noted in in Groups Groups 3 3 22778866..00001155 3MMAA002042848330000 I 1 I11 @HAZLETON Nusome ronesmenos te: ss I Su- - 11 - 154-159 I and 4. The decrease in the Group 4 animals was significantly different I included tn statistical evaluation. 1 tthhaann tthhee ccoonnttrrooll vvaalluuee.. BBeeccaauussee ooff hhiigghh mmoorrttaalliittyy,, GGrroouupp 55 ddaattaa wweerree rnoott included in statistical evaluation. I Food Consumption ~ 1 IInnddiivviidduuaall aanndd mmeeaann ffoooodd ccoonnssuummppttiioonn vvaalluueess aarree pprreesseenntteedd iinn I fopendix 22.. MMeeann ffoooudd ccoonnssuumppttiioonn vvaalluueess aarree pprreesseenntteedd in in Table Table 3. 3. 1 Appendix SH decrassad food consumption values were noted for Groups I 4 and 5 cSolmipgahrteldy tdoeccroenatsreodl foonodancdonasfutmeprtiDoany 8 of valuesgewsetraetinoont.ed Mfeoarn Grtooutapls 4 and 5 compared to control on and after Day 8 of gestation. Mean total food consumption values for Groups 2, 3, and 4 were statistically I1 comparable to control. Total food consumption for Group was not comparable to control. Total food consumption for Group 5 was not I deternined because of orally. determined because of mortality. I 1 Gross PathSoulmoagryy gross pathology findings are presented in Table 4. I YSoummgarroyssgroTsestopnasthowleorgey fnionteddingast arneecrporpessyentfeodr inanTiasballes 4f.ron the No gross lesions were noted at necropsy for animals from the 1 control group or se Groups 2 and 3. Findings noted for Group 4 anil vere discolored Tiver, Kidney, and adrenals; enlarged adrenals; dilated control group or dose Groups 2 and 3. Findings noted for Group 4 animals were discolored liver, kidney, and adrenals; enlarged adrenals; dilated 1I rena bland vessel, and ed vaginal Gichorse. Findins rated for Group S aninats were reddened and/or distended lungs, enlarged and/or friable renal blood vessels, and red vaginal discharge. Findings noted for Group 5 animals were reddened and/or distended lungs, enlarged and/or friable I liver, enlarged adrenals, discolored and/or thin glandular gastric 1 mmuuccoossaa,, fflluuiidd iinn ssttoommaacchh oorr iinntteessttiinneess,, eanndd rreedd vvaaggiinnaall discharge. discharge. I 1 erine eignts I Uterine WIenidgihvtsidual and mean uterine weights are presented in Appendix 1 3. SumrIynduivtiedruianel waenidghtmseanareutperreinseentewdeiginhtsTaalre 5.presented in Appendix 1II 3. Summary uterine weights are presented in Table 5. 22778866..00001166 Swaouzsesor 3MA00248301 I 1 I 1 HAZLETON visomronessemaine I 1 m---------- Sie - 12 - 115544--115599 I Mean terminal body weights were comparable for Groups i, 2, and i compared to control animal weights. There was an approximate 9% decrease 1 33,, bbuutt't`hthee mmeeaann tteerrmmiinnaall body wweeiigghhtt ooff tthhee GGrroouupp 44 ffeemmaalleess wwaass ddeeccrreeaasseedd compared to control animal weights. There was an approximate 9% decrease 1I 1 the sean gravid uterine weights of the Group 2, 5, ard 4 mimals con pared to the control mean weight. For Grow 2, this may be attributeble in the mean gravid uterine weights of the Group 2, 3, and 4 animals compared to the control mean weight. For Group 2, this may be attributable I1 one dim ith it early resending fetuses. Terminal body weight to one dam with eight early resorbing fetuses. Terminal body weight 1 Sinus gravid uterine weights were comparable for Groups 1, 2, and 3, but minus gravid uterine weights were comparable for Groups I, 2, and 3, but I that weight was decreased in the Grow 4 animals compared to the control that weight was decreased in the Group 4 animals compared to the control II vvaalluuee.. I 1 RESULTS RESULTS = - CESAREAN OATA CESAREAN DATA ! 1 IInnddiivviidduuaall aanndd mmeeaann oovvaarriiaann,, uutteerriinnee,, aanndd lliitttteerr ddaattaa aarree presented presented iinn AAppepnenddiixx 44.. IInnddiivviidduuaall aanndd mmeeaann lliivvee ffeettaall ddaattaa aarree 1! presented presented iinn AAppppeennddiixx 55.. SSuummmararyy oovvaarriiaann,, uutteerriinnee,, aanndd lliitttteerr ddaattaa aarree _ pprreesseenntteedd iinn TTaabbllee 66.. LJ PPrreemE_a~rnncicycya RRaartetees,sr,orCCoRorropgororrtaaLuLLuuttteeesa,,, UUUtteteerrriiinnneeeImIImnpplplaalanntntaatattitiiooonnnss,s,, aaannnddd InIImpmplplalaannntttaaatttiiiooonnn Preguancy Efficiency Pregnancy rraatteess wweerree 110000 ppeerrcceenntt ffoorr GGrroouuppss 11,, 22,, @anndd 33,, aanndd 85.7 85.7 percent percent ffoorr GGrroouuppss 44 aanndd 55.. TThhee menmean nnuummbbeerr ooff ccoorrppoorraa lluutteeaa aanndd tthhee mmeeaann nnuummbbeerr ooff uutteerriinnee iimmppllaannttaattiioonnss wweerree ccoommppaarraabbllee ffoorr aallll ggrroouuppss aas5 wweerree mmeeaann iimmppllaannttaattiioonn eeffffiicciieenncciieess.. 22778866..00001177 amaonzssscz 3MA00248302 1I I1 EHAZLETONwsorsronss enon we. 1 I m------ ne - 13 - 115544--115599 I I FFeettaall VViiaabbiilliittyy,,.. WWeeiigghhtt,,...aan~dd SSeexx I for the control group, but these differences were not statistically 1 MMeeaann rreessoorrppttiioonn iinncciiddeenncceess wweerree hhiigghheerr ffoorr GGrroouuppss 22 aanndd 44 tthhaann for the control group, but these differences were not statistically I1 Sinificant, The increased tcidarce in Grow 2 may be stiributable to ane dm with eight resorbing fetuses. Mean incidence of fetal viability significant. The increased incidence in Group 2 may be attributable to one dam with eight resorbing fetuses. Mean incidence of fetal viability and percent males were comparable for Groups i-4. Mean live fetal I1 weights of both sexes decreased with Increasing dose Tevel with Group 4 weights of both sexes decreased with increasing dose level with Group 4 I values significantly over than contr values significantly lower than control. i sea pero: Fetal Development 1I Individual visceral findings are presented fn Append 5 fndivi- Individual visceral findings are presented in Appendix 6; indiviI dduuaall sskkeelleettaall ffiinnddiinnggss aarree pprreesseenntteedd iinn AAppppeennddiixx 77.. MMeeaann iinncciiddeennccee vvaalluueess 1 for visceral and skeletal findings are presented in Table 7 (based on for visceral and skeletal findings are presented in Table 7 Ibased on ! nnuummbbeerr ooff ffeettuusseess)) aanndd TTaabbllee 88 ((bbaasseedd oonn nnuummbbeerr ooff lliitttteerrss)).. 1 Gross extarnal exesimation of fetuses at cesurean section Gross external examination of fetuses at cesarean section I revealed are abrornal fetus from a Group 3 can. This fetus had 0 tal revealed one abnormal fetus from a Group 3 dam. This fetus had no tail ! and appeared to have no thoracic or lumbar vertebrae. However, no 1 Skeletal evaluation was done on this fetus so that Finding could not be skeletal evaluation was done on this fetus so that finding could not be I verified. verified. I1 Setetal exanination of the selected fetes revealed tht the incidence of skeletal variants vas comardble for the control group and Skeletal examination of the selected fetuses revealed that the incidence of skeletal variants was comparable for the control group and I1 ented oro. lo sels] aoneles vere rotss, The fncdece of 1 visceral variants was markedly hier for Group 4 when compared to the treated groups. No skeletal anomalies were noted. The incidence of III11 visceral variants was markedly higher for Group 4 when compared to the 22778866..00001188 Swaouzsesos 3MA00248303 I 1 11 HE AZE LETRO-- N ueommonesaves =ne. rn I 154-159 I 1 ccoonnttrrooll ggrroouupp ((5500 ppeerrcceenntt vvss.. 55..5566 ppeerrcceenntt)).. HHoowweevveerr,, oonnllyy ttwwoo GGrroouupp 44 1 lliittteterrss wweerree aavvaaiillaabbllee ffoorr eevvaalluuaattiioonn ccoommppaarreedd ttoo ssiixx ooff tthhee ccoonnttrrooll I ggrrooupp.. VVaarriiaannttss nnootteedd wweerree ddiillaatteedd rreemnaall ppeellvveess ((GGrroouupp 22)),, ddiillaatteedd 1 ureters (Groups 1 and 4), and incompletely descended testes (Group 4). ureters (Groups 1 and ~), and incompletely descended testes (Group 4). I Cleft patate was found in two of six Group 4 fetuses examined by Cleft palate was found in two of six Group 4 fetuses examined by Wilson sectioning. That anomaly was noted in one fetus from each of the 1I two Hitters fran rich the fetuses selected far visceral examination vers two litters from which the fetuses selected for visceral examination were I derived. No other visceral anomalies were observes. derived. No other visceral anomalies were observed. 1I 1I 1I 1I 1I 1I I 1 I 1 1I I 1 1I 22778866..00001199 awaoo2ees0s 3MA00248304 !LI ae - 15 - ! 1I Be ~ 1I ama Fa oa ! :I fil = . I Sf ~ ~ I I I 1 # ud- - 1! EB. - i :i - - 1 2 ! Ho . on I = I 1 - b PoE ! qi i ! 1 jPileediliiibliiBeiBePna:FEk : ! I 1 22778866..00002200 -- 3MA00248305 1 --1165 -- 1 i 3 f gz af it fI y 1 Fo a La 1 5 I Vo HERR I1 I ! I0 1 gd EEAR 1 rl EAC RE I! 5 | 52 88 23 af 1I Fi ie gue gn gar I 1 i JE[1 877 BST 8ST EET ge I 2 | 8 oF of oB ne I 1I i 38. 2582 228 o0 f 38 1| II BR RAT BRT ge 3 I 1 | 2 #8 58 ef a8 RTC 9 9 BR BR. i 1I g I 1I HACE I 22778866..00002211 3MA00248306 1I -17- I 1 1 a2 g 1 3 By fLiadl ETal LLEa i. Lo 1 z :1 a | | 2g8e vg8re =gon omfde re 1 & 23 | 22 dre gf ge =E nen kf die 110 i 3 23 < 3 !1 z3i3s oga3 2 g2s8 3=33 rRSa 2288 :5 3: Ey] ge ge gin de <de & 1 Zs 9a BoB ae a8 et en a8 3 1 z gre gfe Ads doe ade f i ! 55,8 08 st sh} I ! i I 1I $35=- B3=- B=3- B=3- BB== @ 1 = . 3 i 2 2 2 5 fg 1 22778866..00002222 swsorzissn 3MA00248307 1 Ce - 18- 1I 1I i I 1 oe To ww Wow TEE 1I EE oe an :I gn I I 1 @ I 1 E Sp T ee 1 2 &% I I ek I 1I 5 1 2 : 2 s5z .22 1 5 } Bf g o> :- FH 5 I : GEER ESE Le EE : v I I 1 22778866..00002233 3MA00248308 1: i.- 19 ! 1! 1 "* 1! +. ! 1 "R 1I 1 or |] Sas 3 1 "ts 1 iE si gu; 2 1 1 22778866..00002244 33MMAA0000224488330099 1 i ca 11 - 2O 1g ! 32 i1 sI] p E2E2e 3R8Re g28de gs8an sdfd ve 3 BE 3 ; FETT STR ow 1 Hi i 1 3|- B3i- B3|- HE -BT- | I 3; gz 2 3 og I I 1 11 11 22778866..00002255 3MA00248310 I Cu- - 21 I1I tse Key to Table 6 Key to Table 6 154-159 I 1 PPrreeggnnaannccyy RRaattee ((ppeerrcceenntt)) == ((.nuummbbeerr ooff pprreeggnnaanntt rraattss//nnuummbbeerr ooff rraattss mmaatteedd)) x i00. I1 Sti tate (reap) = (uber of rats suring 0 tay Bymaber of Tats pTacedon study) x 100. Survival Rate (percent) = (number of rats surviving to Day 20/number of rats placed on stud~)""x i00. I1 Mean tnplantat on Efficiency (percent) = Group mean of ({ plantations re Ea Fr fob) Mean Implantation Efficie.cy (percent) = Group mean of ([ implantations per litter/corpora lutea per litte~ x 100). 1 Men Resorption lncidance (percent) = Group mean of ({resorptions per MealniTtEttReeerrs//oiirmmpppt'lilaaonn.ttaattIiinoocnnissdeppn'ecerer l(itpteVietrtercr]]e"nxtx")"l1O0:00))..Group mean of ([resorptions per I MMeeaann IInncciiddeennccee ooff FFeettaall MMoorrttaalliittyy (p(eprecrecenntt)) = = Group Group mean mean of of (Idead ([dead 1 ee atonsrion paTr ar 100) fetuses per litter/impla.tations per I Mean tncidence of Fetal Viability litter ]'k (percent) =100G)r.oup mean of ({live 1 Tomtom Sar Tr 0h: Mean Incidence of Fetal Viability (percent) = Group mean of ([ live I ietus6s per li'tter/impla"tation's~ p'er l'itter] x 100). I I I I I I I I I I I 22778866..00002266 aMA00248311 3MA00248311 I 1 --222-- I | 1I a ] i k ~rgeeo dd 8 i i 1I "PB E~eEgeRq 58S grog Ncheandgs O32 1I + ges BFE FRE BEE OG 1I 1I g5E8 [F egeg a@m9osgZm ggreoez 2 o a25d8d8i8 Ig2ssd Se 2 1I Lii3 + cegeg daiu"ucods gS8o0s8 yoAeKoeEg 5o0f I 23 EB 2= 1I BC gz % =; ii . iFH I fz ze hi I 1i [A gig: 3 EE H SEE GEE ur I i BLUES oop 3p ERRmy ESSE, oF I E5283 532%% 3Ee8E 3:03 EZ 1I1 35853 23253 fssgs 23% 8 GER fii: B==2 2222 S52 a F2% I I 1 22778866..00002277 -- 3MA00248312 1 . Cw. 11 i8 + = gg c8 8 8 s "=8E8 ge 1 b 1 8 08 %3 EEE ! 2 3 2 _8 8. NIB8 5858% 1 os ~5 85% 1 222:3 3E 3 - EId . 1 =:iH :: :: st : P oicias st gEzed f S FI] E- z TF z8Fi:t3 fei: BIg Ii s5s5 jEoSeSc3sEde 3EEf t8o2 33 ge3Esl it g 5353iE3TiE 18882 1 22778866..00002288 -- 3MA00248313 1 1i :a cu +& g3 oei5i eee ! + , 3 ERR 2 1 8 Ams = 1 5 + = 3 Ee oF. 1 _ Voss, i ' hin of i 5s&5f4ze BSc2 5k 1 55 5 1I 22778866..00002299 3MA00248314 I Sos 25- I1 ssa-9 1 KKeeyy ttoo TTaabbllee 88 154-159 I MMeeaann IInncciiddeennccee ooff Visceral Visceral Anomalies Anomalies (p(eprebrccBeennott)) f:=etGGurrsooeuurpp cmmreeaaannmioonffd [(+[ninusumcmbabererarllooffy 1 fppeeerrtuslleiistttteewrri]]thxx ~11n00o00m))~..lies per litter/number of fetuses examined viscerally I eMeaann IIncciddeennccee ooff VViisscceerraall VVaarriiaannttss ((ppeerrcceenntt)) = = Group Group mean mean of of (numer ([number of of 1 fpeeertfuussleeisstteWwirit]thhx vv1aar0ri0ia)na.tnsts ppeerr litte11rt/tder"/un~umbbeerr Ooff ffeettuusseess eexxaammiinneedd vviisscceerraallllyy per litter] x 100). I I MMeeaann IInncciiddeennccee ooff SSkkeelleeeettaallboAArnnoommaatlleiieesss (p(eprenercacreennatt7)) f==etGGurrsooeuwspp xmmaeeaannninooeffd ((S[[knneuurmmebbenerralloofyf I fppeeetrruslleiistttteewrri]]thxx a11n00o00m))a..lie~'p~r litter/number offetuses examined skeletally I i MMeeaann IInncciiddeennccee ooff SSkkeelleettaall VVaarriieaannuttss m(bp(eaprerrccoeenntFt)) f-=-etGGurrsooeuuspp ammeveaaannntnooaffd ((sI[kmneuuTmmebbeevrrallooyff pfpeeerrtuslleiistttteewrri)lthxx v11a00r00i))a..nts per 'lifter/number of fetuses examined skeletally I I I I I I I I I ! I 22778866..00003300 M3MA0A000224488331155 I 1 cu - 26 - I 1 i1! 82 "f4F ~eggeg8 "18E8S8E3 weongg 2g3eo I I 1 = + rocco ocooo noong8 5H8gec I :F i i11 25 + coongsodphos weoesg oJyges II1 i f59ss 35 3% 3 5 3 I 53 &% ,8% ER i 1I 58 SBisEEaisye IHrEEaf I =z Esfsf SLs Es@sf iiss I 3338 3848s 33532 38:35 I T3T5U5T0y g8p5f3p5E8 TBoIoIStD g5p8%d3 I I 1I I 1 22778866..00003311 3MA00248316 - 27 - I - 154-159 KKeeyy ttoo AAppppeennddiixx I1 Individual and Mean Maternal Body Weights Pilot Rat Teratology Study of T-3352 1 + pregnant P = Pregnant I 8 NP = Not pregnant GC RETIN porta (sep i of station) C = Cesarean section performed (preceded by day of gestation) FD : Found dead (preceded by day of gestation I AA == AAbboorrtteedd ppuuppss ((pprreecceeddeedd bbyy ddaayy ooff ggeessttaattiioonn)) I I 2a Booddyy wweeiigghhtt oonn ddaayy ooff ddeeaatthh.. b Statistical group comparison performed on rank-transformed data. I NNoottee:: BBooddyy wweeiigghhttss iinn ppaarreenntthheesseess aarree eexxcclluuddeedd ffrroomm mmeeaann ccaallccuullaattiioonnss and statistical evaluation. I I I I I I I I I I I I 22778866..00003322 3MA00248317 I . a 28- 3IVo: gfi mm? mad I 2s geome 85 eeean~ 8 I 1 i #ss8s gRINES 93 1I 1 ) i wFaemrarnis r 88 sze owas 8% I1 |iPI a:d mmBE BREE 1 2E2E 3 28 amane -32 03 .3 _ 1 iBis1:jmEn hE mE m 25 9 | pnvae BY wenaue 23 I 3 | yagmen 88 sanany 2 1 3i | sg%a8 E=%aRE 8 1 Z| I 1 1 22778866..00003333 awocsess 3MA00248318 1 a - 29 - i1 ii i3y BEaE 2331% oe 1I 4#3 cacesss 3 5 Tux G4e 1I 5 REE EE fe = 1 | ommad iiEE 1I i gta ad I I i2 anannen 35. e3te-s 83. 1 I 3i14 gagagds 5 E3NMEdE 1 iBRol2g mRadmeRmE R8 ag AmREsRR 8 I 1I i H: HE Hide I ! 2: 8 1 ennees 38 snesnus af 3 4 EEE EEE 1! 1 Di o|o: : aHmEouHsaEs 85 HsneEnnEes= 28 i I 1I 47 1 EE I I - .-I 1I 22778866..00003344 Swaouzsesia 3MA00248319 I 1 ep- 30 - Iog 1i i 1I 43 I 1 II1VoPl l| aaae 1 5g I w3 l E--E--E i 1 Ey 1I AEE i fF 1!bE #3 a&md 'I PH smd I ! [i 1I El ! 1I 1 jg mann I 1I 22778866..00003355 3MA00248320 1 cae 1 1;& 11 2a 8F 9 EsuoE uey BH po 23 hi 3 1 5 1 i 1: iBE | : oR=E=R9H%RaT 2a8 nFaenwddsd e3s8 1g 1 553.2s 3 fy { < |. nsSouesndneew 8a8T ETB.EE s3 El Vg 1 ACEH E Ls ianntiamd89 : 3 gdnsy BE EP Ry = Lf 1 ol zg SuRngEeSs a4 wsiodndosgsn g G&gL 1 2 | 4m8o33m33 2% a defm ied 8 38 1 1 1 i 8338gs8 gs 3 BIEEEE S%S8E% 5 5 i 3 1 2 1 1 2786.00 2786.0036 [te 3MA00248321 1 w- - 32 i IrT a I 1 2 Lo I!1 3 : | cwnmmmn SB ~B<8Get 93 .o I 1 Ex g i 23 33 I 1 JF | gussans BE zauaizE BO I 2 gc fi .-es if ! 1I EE Boo : 5 is I | f onenasy TB qoronem RE 52 33 I SngNngs d4 6 ddgencd dd 33 1I FFE smmun ocak HE TT TTT Bg 11I = | s conmmanee B88 daemdeaannacsd a855 8BX I i= . : 1 22778866..00003377 3MA00248322 1I I 1 1I I1 3 I II we - 33 - 3 oo ; SH dings z 3* Z2ININS : ! I I SE EB |F gzznzdEuzszeanz f3 o 1I1 Ee3 32 sI323I833E33%E z: ! s%23fp 3 Fl! fe SIEBREEEIEEEEE Z5 !I B2s7, 2sE 85 gead=nge 8a8 3f 1! 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I 1 I |: I ! 18 II1' :i I 1 =B I I 1 H 3 e3 3. 2g 1H I I1 id I I gemuwae 1 { 5% se I . : I I I 1 | I I 1 22778866..00004477 3MA00248332 eet gen I1 Cae 43- 1I i =e med 3 I 1I ! L=|I v 8 p --2- I ane] me Eo 8 I II mfme c Seed dese3 1I 3 mer] ne 3 -3 en] me 3 -3 I 5Fob t 03 geen] me .do3. bo fhm 22 Jods o3 I 327 | I - - Voom 1l Fob ees o8 feedees 03 I HO i Hi z 11I 3F2 is3 3I HE fi 3 3i EI I 2 Bi 3d I 1 emg io gezf , B2 EHs ioii uis . perf, 3 iri I 1 iiantiif Efasgioin n2n:d, f I 1 gi pind git I I 1 22778866..00004488 SwAouzsesss 3MA00248333 ! 1 c-44- 1I i edd = 2 ! 1 1 Er fog - =e I I i 3 3 i | #n el -3 I 1 |a 3! I i cere ng mT - c i II1I E2Bl3 dleeene?d- . - Ee3Nli I 1I II i F33iBa2E lifa,h 3i1 di 1I f$bi mislmiEadl, iidos1 1I gglyiamha pa i1. d ITER HEN I I 1 HpEEE fr FH I 1 22778866..00004499 3MA00248334 I 1 -5- 11I i2 3 I Boorfro nd vn oo 03 . 1I 3 . 1 sheen] om wg -- = 3 I 1I I rmeee] fmen+ Fen 3 .3 a I Pood ro : . I I Sg pele - -3 1I =BE ef oo mg8 oem <3. :I oped -3 I I : i d11ioB3d3He. 13 I Bi naib bi I 1I pSrL,EELEERNLEW LH I 1 pps BG ne I I Emr fF I 1I 22778866..00005500 3MA00248335 1 o-- 46 - I Appendix 8 115540--115599 I 1 Pilot Rat TerRARaepetfpfoeeelrnrodeeginnyxcceeSss8tudy of T-3352 Pilot Rat Teratology Study of T-3352 1 SSTTAATTIISSTTIICCAALL MMEETTHHOODDSS/!LLAABBOORRAATTCORRYY PPRROOCCEEDDIURREESS I 1 ABBgaarrrittclleuettlttt,,ureMM.. aSSn..d,, A""pSSpaolmnieeedEExxaaBmmippollleeossgy.oo"ff SSttJaa.ttiissRttoiiyccaaalll MMSeetttahhtooiddssst. ooffSoRRcee.sseeaaSrrucchphplii.nn I AI1gVV:r:i11c33u77-l-11t88u33r,,e 1199a33n77d.. Applied Biology." J. Royal Statist. Soc. Suppl,, 1 RDDerrvaaippseeirr,t,edNN".., RRT..echaannnddoreWWt..riGrc~..s,HHuu1nn1tt:ee2rr3-4((011.9%699)),, ""TTrraannssffoorrmmaattiioonnss:: SSaommee EExxaammpplleess I Revi sited", Technometrics, 11:23-40. 1 TDDruuennannetettmtte,,ntsCC..wiWWt..h,, a ""CAAonMMtuurllotlt.ii"pplleeJ.CCaoAnmnpp.aarrSiitssaootnn. PPArrsoosccoeecdd.uu,rree50:ff1oo0rr96CC-oo1mm1pp2aa1r,riinn1gg955SS.eevveerraall Treatments with a Control." J. Am. Star. Assoc., 50:1096-1121, 1955. I 1 BDDiuuonmmnmeeetttrtti,,cs,CC.. 2WW0..:,,482""-NN4ae9ww1,TTaa1bb9ll6ee4ss. ffoorr MMuullttiippllee CCoommppaarriissoonnss wwiitthh aa CCoonnttrrooll.."" Biometrics, 20:482-491, 1964. I Gw6aainmteehss,,UnPPe..quAAa..l,, aaWnn'dds HHoo"wwaeenl|d/llo,,r JJ..VarFF..i,,anc""ePPsaa:iirrwwiiAssee MMMouunllttteiippllCeearCClooommppaaSrrtiiussdooynn."PPrrooccJee.dduurrEeedss. wSSitttaahttiissttU..n,,equ11a::l111133--N'11s2255,,an11d99/77o66r.. Variances.- A Monte Carlo Study." J. Ed. I 1 LCLeoevvneetnnreei,,butHHi..o,,ns""RRootbbouussPttroTTbeeassbttisslitffyoorr aEEnqqduuaallSiitttaytyisootfficVVsaa,rriiaaSnntccaeenssf,,o""rdiinnUnIIi..veOOrTlskkiiitnny eeddPiirtteeesdds,, I CPaolnotFRrIiAGblATtuEotS,i,on1s960t.Toom, Probability an.d SSntaat.ltaitstiicces,, Stanford University Press, 1 SSitntaapptllheeess,, KROR..H-AEE..liazannaddrinSSccRhhenndeellllS,, MVVe..thLL.o.,,d ""fRRoeerffiinnFeeemmteeannlttssSoiinnne.RR"aappiiddStCCallieenaarriiTnneggcnTToeelccohhgnnyii,cc I i33n99::66t11h--e6633,,KOH1199-66A44l..izarin Red S Method for Fetal Bone." Stain Technology, 1I A WTTieeclchshnoniniq,quueess,J., TTGhh.ee UUannniidvv.. WooaffrkCCahhniiycc,aaggooJ.PP,rreessessd,,itCoChnriiscc.aaggoos,Ter1a9t6o5l,ToEobs qppyph:.e 2P5r1i-Bn2Rc7iI7pT.loes oand 1I HWWeiibnnreearr,,r-HBiB..TT,JJ..,,RoSSotctaattiissChttoeiiccaaSllTO,PPrriinncciippTO lloeesso iinn EExxoppeeerrriiimmneeennntttaaalllfeDDesessiii.ag~nnn,, 22nndd EEdd..,, McGraw-Hill, ~.Y., Chs. 3-10, 1971. 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