Document 5x2mwrn9rOXQxD9OQZyzBx7e
ACUTE TOXICITY TO AQUATIC INVERTEBRATES TEST SUBQ@TANCE
(DAPHNIA MAGNA)
Identity: A mixture containingperfluorooctanesulfonate,which may also be referredtoas PFOS, FC-95, or as a component ofFC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was not noted.The testsample isFC-206, identifiebdy the laboratoryas "Sample C". Currentinformation indicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether, 78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate0,.04% sodium laurylsulfate0,.5% polyoxyethylenemonooctylphenylether,and 0.05% benzotriazole.
The followingsummary applies toa mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemicalcomponent of the testsample.
METHOD:
Method: Methods forToxicityTestswithAquaticOrganisms as formulatedby the Committee forToxicitTyests withAquaticOrganisms Type: Acute static GLP: No Year completed: 1974 Species: Daphnia magna
Supplier:In-houseculturemsaintainedatBionomics,EG&G, Inc., Wareham, MA. Analyticalmonitoring: Not given. Exposure period: 48-hours Test organism age: 12 -24 hours old Statisticamlethod: TL50 (median tolerancelimitv)aluescalculatedusinga linear egressionequation. Test conditions:
Dilutionwater: Well water,aged 24 hours Dilutionwater chemistry: Not given Lighting: Not given Stock and testsolutionpreparation: Not given. Exposure vessels: 250 mL beakers with200 mL exposure solution Number of replicates:4 Number of daphnids per replicate:5 Number of concentrations: fiveplusa blankcontrol Food: Food, consistingofa homogenization ofstartertroutfood and Cerophyl inwater,was added atrateof0.5 mUL afterintroductioonftest substance. Water chemistry during the study:
Temperaturerange(0-48hours):20 1 "C RESULTS
NominalconcentrationBsl:ankcontro3l2,00,4200,5600,7500,and 10000 mg/L.
Elementvalues:
48-hourTL,50=5850(4810-7110)mg/L 48-hour NOEL = 3200 mg/L
Element valuesbased on nominal concentrations.
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The valuesreportedapplyto thatmixtureand not the fluorochemicalproportionalone.
CONCLUS
S
The FC-206 48-hourTL50 was determined tobe 5850 mg/L witha 95%
confidenceintervaolf4810to7llO mg/L. The 48-hourno observed effect level(NOEL) was 3200 mg/L.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking= 2.Thisstudy meets the criterifaorquality testing.However, the sample puritywas notproperlycharacterizedand the studylacksanalyticaclonfirmatioonftheamount offiuorochemicalproportion inthe solution.
REFERENCES
Test was conducted by Bionomics,EG&G, Inc.,ofWareham, MA atthe requestofthe 3M Company, St.Paul,MN, 1974. OTHER
Last changed: 6/28/00.
BIOASSAY REPORT SUBMITTED,TO 3M COMPANY
ST.- PAUL, MINNESOTA
ACUTE TOXICITY OF SAMPLE C TO WATER AND SCUD (jammarus fasciatus).
FLEA
(Daphnia
magna),
Bionomics E G & G, Inc.
Environmental Consultants 790 Main Street Wareham, Massachusetts August, 1974
METHODS AND MATERIALS
These investigations were performed at the aquatic
toxicology laboratory of Bionomics, E G & G, Inc.,
Wareham, Massachusetts. The susceptibility of water
flea (2ap]lniamagna), and scud (Gammarus fasciatus)
to Sample C, a brown liquid, was evaluated under static
bioassay conditions for a 48-hour period. Results were
expressed as the median tolerance limit (TL50)* the
nominal concentration of the test compound in.water
causing 50 percent immobilization for the Daphnia
and Gammarus.
The TL -Value and its 95% confidence .50
intervals were calculated by converting the test
concentrations and the corresponding observed percent
mortalities to logs and probits, respectively. These
values were then used to calculate a linear regression equation.
Procedures for static bioassays as described.in the Methods
for Toxicity Tests.with Aquatic organi-sms as formulated by the Committee for Toxicity Tests with Aquatic Organisms were followed, except for those conditions described below. The 222hnia, and Gammarus were acquired from laboratory cultures.
The Daphnia and Gammarus bioassays were conducted at
20 0 c
1.0). The invertebrate tests were conducted in
Page two 250 ml beakers containing approximately 200 ml of well water which was aged for 24 hours. Five 12-24 hour old D?_phnia, and five.fourth instar Gammarus were placed in each beaker' (4 replicates/concentration). In the Daphnia bioassaysi Daphnia food1 was added at a rate of 0.5 ml/liter after the'introductionof the compound into the test vessels. RESULTS The predicted TL50 values and 95% confidence intervals for the Daphnia and Ggmmarus are presented in Table 1. Table 2 presents a summary of observed immobilization for each individual test after 24 and 48 hours.
Food was prepared by homogenizing 5 g of starter trout food,and 1 g of cerophyl in 100 ml of water.
SUBMITTED BY:
Bionomics E G & G, Inc.
Environmental Consultants 790 Main Street Wareham, Massachusetts August, 1974
PREPARED BY:
Robert E. Bentley
Chief, Acute Toxicit Investigations
APPROVED BY:
Bevier Hasbrouck Sleight, III
ddw,
Director, Freshwater Programs
Table 1
Acute toxicity of Sample
b
Gammarus..
The data are
C to DRU !Iniaa and based on results
of bi oassays.conducted at the fish toxicology
laboratory of Bionomicst- E G & G, Inc., Wareham,
ma ssa chusetts.
Species
TL 0-milligram active ingredient/liter
24 hour
48 hour
No Effect Level
(mg/1)
Daphnia Gammarus.
5109-000 5,310(4,340-6,510)
5,850(4,810-7,110)c 3,200 5,170(4,190-6,370) 3,200
a Bioassay conducted at 20 0 c at the initiation of tests.
b Bioassays conducted at 200 c at the initiation of tests.
c
95% confidence intervals.
1.0), Daphnia 12-24 hour.s.old
1.0) Gammarus 12-24 hours old
Table 2
Concentrati*ons tested and observed percent immobilization for Daphnia and Gammarus exposed to Sample C after 24 and 48 hours.
Species Daptnia
Gammarus
Concentration (mg/1)
10,000 7,500 5,6oo 4,2,oo 3,200 control
lo,oob
7,500 5,6oo 4,200 -.3,200 control
immobilization observed 24 hour
20
100
25
50
0
40
0
10
0
0
0
0
100
100
80
80
6o
75
45
50
0
0
0
0