Document 5oYgmY6oZXJme3E4KKvX8QR8

A fO O irW jL BIODEGRADATION (BOD/COD) TEST SUBSTANCE Identity: Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafluoro, ammonium salt, CAS # 3825-26-1) Remarks: The 3M production lot number was 37. The test sample is FC143. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 96.5 - 1 0 0 % test substance and 0 - 3.5% C6, C7, and Cg perfluoro analogue compounds. METHOD: Method: Not noted. Type: Aerobic GLP: No Year completed: 1980 Contact time: 20 days Inoculum: Sewage taken from the treatment system at 3M Chemolite, Cottage Grove, M N . . RESULTS Chemical Oxygen Demand (COD): Biochemical Oxygen Demand (BOD): 5-Day: 10-Day: 20-Day: 570 - 870 mg/kg* <38 mg/kg <38 mg/kg <38 mg/kg * Two tests were conducted. The raw data sheets are not available. The summary sheet states "a fraction of the sample went up into the condenser each time, so the results are not consistent when run in duplicate". CONCLUSIONS No reliable conclusions can be derived from this study. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 3. Study lacks description and records of the method followed. The inoculum is not properly characterized. There is no 003683 analytical measurement of the test substance in the solutions. Test substance was not characterized for purity. REFERENCES The COD and BOD studies were conducted the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number 5625S, 1980. OTHER______________________________________________________ , Last changed: 5/24/00 003684 Form 10620-5-PW O coi LAB REQUEST BOOK - 42-5W PRODUCT ECOLOGICAL ASSESSMENT REQUEST FOR LABORATORY WORK RjJ: ENVIR. ASSESS. INga, #_____________t______ DIVISION PRODUCT: c - - / < / 3 ~llt - 3 ? c c &QU~=:?r LAB REQUEST NO. REQUESTER LABORATORY m cttmrEQOPi "F Reauest Date: / / / / fP O ProiectNo.: < 3 1 * ? t a i 1 . 0 0 Prob No. Code No.: Sample Date: 7 7 - l l - t Dite Needed: DescriDtion: C, " / V 3 L o 7~ r Purpose of Tests: r rw S c / < f O J. #& Cawf'i/'pvt Analyst: *( L Date Complte1: Hours Spent: M p " Y B' lA T g - W ' ' < Z - S - '3 H '> AS Please Call Reauester If Delays Are Encountere il. I6 x -rs -rr-o PHYSICAL & CHEMICAL PROPERTIES H e a to f C om bustion C al/G (B T U /L b .) D ensity Ash O ther r C - !W L o r 37 WATER POLLUTION -P H - C . O h COD BOD5 L l/ P 1/ BO D , U ltim ate A TOC ' GC ^ O xygen U ptake ToxtciS & s U J j < - Dehydrogenase A c tiv ity (TTC ) O ther 570 n / Jb K 2 70 3 3Y < n H /k & X 'r M / k f J (/ (J X K A *C * *r> 4 \ fl A-- te ` /* > --/ 7, oC * 5 rO BIOASSAY L<Lso Specify Organism and M e thod : /4 LEACHATE FROM LEACHING TEST PH COD bod5 BO D , U ltim ate TOC GC Bioassav O ther K 7 C 7 V 3 -7 y ' / / 1 OTHER B ioaccum ulation - 7 Persistence D egradation Products ------------ r y ------------- ~ 7 ; . Z TM s rf L //t W hite - R eturn to Reauester C snsru . E ~ - * J Ml* P ink - E nviron m en tei Enora. C03 6 8 5 > / e y i'ij j&KSStt L I M r k M * - & e tia , oh, J------------- --- Raquast N o. -- Typ 4 toure - 5 z5 5 BOO Run No. | Data Startad Y -Z S -% 3 *7* M Coeeentretton ^ ^ (S u o -l-Z V -_____________ <gQ _. j f j - O ay BOD Readings - #i V i V i V O VI BeMaNo. MtWiAOt)O.. FreelAwji)tDA D.OlA.D|e*pAlejtlion ^ - ^ 'l ' Y .0 5 Z .O O .6 S 1 , V5 . 50 >eaded Dilution Water B link (optional) B ottle No. >/ 3 U Controls - In itia l 0 /) . < C ,I Preaani 0 .0 . (C s) 9.00 K. 9 5 r.7 S ?,V<r 9. o______ i 5o________ 0 .0 . D epletion IC , -C 2 ) 0.5 5 OW. 0 .5 0 Bottle No. TT9 x! 93 ____ L____ Sample State Z 40 6d C ) I Z Q , ____ in itia i 0 .0 . 1 # ,< / s Z 30 7 .9 0 9 . 2 S ____ _ Praw nt 0 .0 . lB*] 9 .7 S n. 3 5 7 ;O 5 .6 5 Glucoee Glutamic Add Standard B ottia No. Semple Size /<? 3 .0 / 5-0 --25-- . . 7j O .. In itia i D.O. (0 ,1 <o2>Present 0 .0 . <?0 l 95 m . ______ 5: 7-0 3 ,4 0 Total 0 .0 . D e p le tio n (0 , - 0 2) 2 .Z S 3, 75 5. SO O apiation Oua To Standard A7 / o ..\ 1-97 . Potassium Hydrogen Phtkalata Standard (optional) B ottia No. J Sample S ite ' In itia l 0 .0 . ' <0,1 Praaant 0 .0 . <02 l T otal 0 .0 . O a p ia tio n (D , D2) O apiation Due To Standard ' AD(eaeplrai-afetio20n).0. 0 .5 3 Average 0 .0 . -O ap iatio n (C i C jl o .^ (B,D.O. Oapiation B2 ) 0 .70 H 0 -90 i /, 60 < s*i 3" . Qay BOD p A v tra g i 5 -D ev BOO 171 /q 3 ;; 2n o 2 /3 " Day BOO /ru*^* Avaraga Day BOO r o im 19755 PWO 0036S6 ^ -Vi1*-- -1 aJZGr 5 <rj ACs3 .7 M U J l ta, fYY m ^ .lai Ljtoratory Shwt dottitelo. MtiaiO.O. U ,l fnmtOA. (Aal D.O. Captation (A, Aji m < p ...... Y. 5 0 - /> > & 50 ST - fistiati O totoi Wtm Blank (pptiond) *" 1 1 1* 1--1 -BotttoW Initial DA. ,) 7.9ST % '0O .9 PraaantDA. a 0 .7 S 37 n .6 o ~ DA. Dapiatlon ,-Ca 3 . Vy " Seed Controls ' 9 ,0 0 X .Z S V .H p gV70 9 . g o ___________ 0 ,6 0 (W E n ./iS . Botda No. initial 0.0. ,) frasant 0A . a T f *7 0 i <73 2o ______________________ / o __________ Glucosa - Glutamic Add Standard # .< & 2 7 .9 0 Z 2S - 7.4 ? 6 .2 0 <b>, 0 5 " 9.5 5 B otti No. "^ /3 I S am plaSiio / In itia l D A . (D ,l 5 .0 7-0 $ .9 0 f .95 2 .9 0 Araaant D A . (O j) 25' ..4 0 T otal 0 .0 . Dapla tion ( D i'D 2) ^ . Co5 4 -so 6 .5 0 Captation Qua To Standard Potassium Hydrogen Phthdata Standard (optional) BotilaNo. J Sampia S ita In itia l 0 .0 . U>t> Praaant 0 .0 . (o2) Total 0 .0 . O apiation (0 , - D jI O apiation Oua To Standard AvaraeO.O. D(Ae^la*tio2n) O .C ^ Arara 0.0. Captation ie , -e2i 0 *5 7 0A . Captation ia) /. 0 0 ! -50 / . S ' 2 . 7 0 Dov / A ra ta ti BOO -3 L _ _ -0 ty ' BOO Day 635" Araraga - Day BOO 0036S7 ^ oc- S '- CA'K^noi^J, talU 6*eS*y %and Stand** > ,0 R a q u a s t No. S alyat ^ j. . 1 M Vvpn aou/a iW aiw i tff/% "Jihtiw .- BOO R u n No. | /j ^ O M tW M d _ -2 - 0 An*vat //,C rt * i f t n --------------------- *^ 0 " / 7 ' D a y B O D Readings _ a .. Data s //s /m ....O..N..vVtio'-'n-"'Wdtor1 to t* No. >^ totoi frill Wl /> 0 4r y , 0 / >> S - :, v * & >' taidad OSMoii'WaHr Manli (iMNlMd) Prtaint OX). U, ., - ? r .s s r OX). Oaplation (A,.A,i / .9 5 0 .1 5 /-7 S Avaraga0.0. O( ialpl*a^tio2n) ASS MNMdkO. ; '* > h n u OX), al 0.0. Oaplation Kr C,) v 3 ,,. 9 .0 0 tv s - 9 . Q Q . __________ x> . % o __________ 2 .Z O 2 .0 0 2 . 2. 6 , Avaraga0.0. Oaplation 16, C2I 2.13 Saad Control ' BotdoN. . f-/ <73 -- - ........ 2p' 0 /2 Q MMOA 1 vsr &30 7 90 ___ y 2s PraaaiuPJ. a> <i. 20 .2 . S O : 0 .7 0 ! < 0 .5 , DJ. Papladan a V .2 5 5 .? 0 7 -2 0 Giucoao ottlaNo. r frtfr* : T il Praaant0.0. >2 3 .0 , . i-< ? o </. /O 1 5 ,0 r & $ 5 2 .H S - nX ~ 7.0 : f t - 9 0 Potassium H yd ro * H *d d * Siw dMfd (optional) Total oxt. <Ot -0*1 < i 0 6 . SO OaplationDna ToStandard aoo -Daw -JAaw .7* BOO Onv BottlaNo. 1 _y L1 _ Bampla Blaa MW DA 1) Praaant 0 .0 . <o2i Total 0 .0 . Oaplation (O fO a l Captation Dus Day Avaraga -------- 55" Day *"'*9755 PWO C 0 3 6 S8 ^ X * . S - <&l j virbnjxntal Laboratory 3i7 Work Sheet *Samples 1-------------------------:------ :------------------------------------------------------------------------- j R t. N o. 7 5 /0 2 5 < Data Startad B O O R unN o.l 1-- db y / 2 5/% 0 - D ay BOD Readings Data V -3 y K > F orm 1976 PWO -eotol Laboratory 'o jrk Shoot - Somplor 0(toq.N .. . . 1 5 _____ __ BOO Run No b Data Karnd V /Z S /to _ . < 0 - / 7 - Day BOD Readings ~ - s0 ! I A Oat* jr -5 -^ r o np*a Oaaoriptlon Initial Dilution r^H 3 of Samplediluted to . ml with 01 water lnHW7POMhlR Factor (Ollutlon factor - 1 if undiluted) __ *SSI* 2$ 5? .u im >. a it ) PtM M t OJO.IO3! 4 ^Tt f*A? 77 2fl$ * 270 w %-&> 3.61 9 ./a to o 50 X.90 70 u Total 0 .0 . OspIMteii (D , - 0 ,1 --O tpltilfft om ToOMWle KJl/vAvarea -D m 1 BOD <n0 /|CQ w- __ -- fo 'm PVVO 003690 1 onto! Laboratory jrk Shoot Somptoft --------------------- -- b R e q .N o * ^ /0 25 D a ta Started y / 2 S /Z O BOO Run N o # * % ()- \7 2 -0 D a y B O D R ead ings Sampl* Description ......... -!-------------------------------------------------------------- D a te s-yg-SQ Initial Dilution Initial ONirtlon Paetor.. of Samplediluted to ml with Dl Water (Dilution factor - 1if undiluted) " n S!* Cane. ef iMnsiei S* ImMM O jO . <0,1 hw K OJO. <0,1 ToM D.O. V O a" Oil# TaSansda ___ 2c L .*ay OOO ' BOO 77 /t- i S) T- " 'r *' ' k`C-% - f-2 5 " I 3 fc 5 K s y .s s ; fi /o /O / 1Mote: V* -a < t. * a* . -i 1.. Coflfr ' Cobflefttratlonof sample (m l/l or m g/l) In BOD bottles. 2. Sample S its - ml or g o f sample or diluted sample added to BOO Bottle. Comments 's _ e 'ff- -' u 003691