Document 5knv7BYJ9x4wmNY3QXEJbGjKe

ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may also be referredtoas PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonicacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 502. The testsample isFC206CE (2/16/89).Currentinformationindicatesitisa mixtureof 0.92% PFOS, 15% diethyleneglycolbutylether,76.09% water,4% urea,1.28% Sultonefoamer, 1.55% sodium octylsulfate1,.05% polyoxyethylene monooctylphenyl ether,0.06% sodium laurylsulfatea,nd 0.05% tolytriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD Method: OECD 209 Test type: Acute static GLP: No Year Completed: 1990 Analyticalmonitoring: Dissolvedoxygen concentrationpsH, and temperature. Statisticamlethods: Resultswere determined by calculatioonfthe % inhibitioinn respirabonrate. Test organism source: Activatedsludge mixed liquorcollectedfrom the Metro Waste Treatment Plant,St.Paul,MN Test condition: Dilutionwater: MilliporMeilli-QTwmater Synthetic sewage: per OECD method Reference and testsolutionpreparation: A stocksolutionofthe referencesubstance,3,5-dichlorophenolw,as prepared by dissolving 500 mg in1OmL 1N NAOH, dilutedto 30mL with MilliporMeilli-QTM water,then brought to the pointof incipienptrecipitatiownith 1N H2SO4 and dilutedto 1L withMilli-QTwmater. The pH ofthe referencesolution was measured tobe 7.2. Test substance was added directltyothe testvesselsand was apparentlymisciblewithwater. Test vessels: Not given. Number of concentrations: threeplus1 referencesubstance and 2 blankcontrols Temperature: 17 - 19 OC Total suspended solids and pH on day of testing: 7.853 g/L (TSS), initiaplH 6.7 and finalpH 6.9. InitaTlSS in1000 mg/L concentration was 3.14 g/L,initipaHl 6.7,finalpH 6.8,and MLSS 3.34 g/L. ElementBasis:Respiratiionnhibitaisondeterminebdy oxygen consumption. Remarks: Values were correctedto20 OC forcalculations. RESULTS Nominal concentrations: 2 Blank controlsr,eferencesubstance,320, 560 and 1000 mg/L Elementvalues: 30-minuteECw=>1000mg/L 3-hou rEC50 = >1000 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on a mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The FC-206CE 3-hour EC50 foractivatedsludge respiratioinnhibitiownas determined to be >1000 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY Reliability:Klimischranking2. Testingmeets allcriterifaorqualitytesting. However, sample puritywas not properlycharacterizedand the studylacks analyticaclonfirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES Thisstudy was conducted by the 3M Company, EnvironmentalLaboratory,St Paul,MN, Lab Request number H 1480-2,1990. OTHER Last changed: 6/26/00 ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE MIXED LIQUOR Type TestAc:@-jva+PrA Analyst Source Date Obtained ;I@12-A ICIO Mixed Liquor Suspended Solids PH Comments t..I-b--c-iri1A.l,q (MLSS) Date Used- ci o g/L Adjustment g/L Test Substance Sample Description CE L.-iW-, (ZjliQiea) "htbi- COACCV*XftUOV,'tCakiMiloooniL,- Sample p Adjustment CommentaMLS--j &,p u Reference Substance Sample Sample DescriptiorL,@IA, V@3kC,-& 000.CXKtr%UOVttCdtAdj%On-BiL - pH *%vj.Agtip,p@ -gy,@d 11-0 ) Adjustment Comments Exposure Conditions Test Duration, Test Temperature 11 -[Ci 0 c Comments in- lo -a -o Pwo Sample Description Environmental Lab - Work Sheet OEM ACTIVAIE6 SLUDGE RESPIRATION INHIBITION TEST #209 Lab Request No. Page- of Date: Analyst: 11 --3,Lth.%f-CAtp-cln MRFP 7- Test Conc. mg added iilp/l riar q 0 ik Arm ah 05,@ IT ml mo water ml syn. sewage ner d nt-r -SOO mi + P.RIL + 4 +2 4- 4- + ml microbial innriilivnt-r 2 t)C) 2nn 2)00 zr)o Total volume MI d.='n,iA -Lna,-tci,AtL rA4- LL--,M :E'Ov-04.e&S Iei%A -rrns LR ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 RAW DATA Test Substance:;7('- Z-Ola:CF, L>o+-wc)z (-zltt,,Isci) Source of Activated Sludge Mixed Liquor: \A, -F Date Received:- -:SI7.5/cin Date Used: -51 Analyst:@@- 30 - MINUTE EXPOSURE - DISSOLVED OXYGEN CONCE TRATIONS ma/i iest Substance TI@IE MINUT S Concentration(mg/L) -0.5 1.0 1.5 12-O.@0@k22.551 13.0 3.5 4.0 14.5 5.0 5.5 c CONTROL 1 1.4 I15. C)8. 1 L4.L *-a"si. A.1 \. n t,.c) 4.in L4.o ? -Z ol CONTROLI-I 14,C)IA.,S12-.q.A.5 I,q 3 - HOUR EXPOSURE - lest Substance Concentration(mg/L) 0.5 CONTROL I DISSOLVED OXYGEti CO@ICENTRATIONS mg/L 1.0 1.5 1 2.0 12.5 1T3IM.E0-MI1N3U.TE5S 1@4*.00#4-*-54.5 5.0 1--5.5 TOEC@ LD, I "Is.10 4.q L4-.4- --@2LL- i's 1,C) lci A?.() 9%.-l -9.21 -4.lo 4.1 A.-c@6.0 ?,,L41.9 1---xt3l.Q lp -t@,9 5.:@s 4.-1 1 4.?. -*isI. z6s 2-.o i.L4 i.c@ if-)Oo Z3.5 I.Cl 0. 8 C? CONTROL II pH of Activated Sludge Mlxed Liquor: pH of highest concentrationtested: Test Temperature:Il-19 OC Initial to."I Initial Final (P.q Final ip,8 Test Substance ACTIVATED EWIRONME-NTAL [ABORATORY SUJDGE RESPIRATION IMIIBITION CALCULATION OF RESULTS TEST #209 30 - MINURE EXPOSLJPX. Test Substance Concentration(mg/L) MNTROL T Oxygen Uptake Rate R '7Io!i R Corrected(') to 2e C 1,180 LR -W 11-4[90 - Z, Date Anal)rsi@*L I Inhibitionin Respiration Rate(2) Lk ,i AYNMOL I1 1.1-2.l1q Percent Difference in Controls(3) BCSO (9SI Confidence Limits) mg/L Test Substance Concentra@ion (mg/L) COMMOL T 0 Oxygen Uptake Rate R 9 j%q I-OS-(DI @l IID9LI 1. R Corrected(') to 2e C I Inhil)itiion Respiration Rate(2) 1.I-RZ-s -?-E@ CONML-II Percent Differencein Controls(3 BC,O (9st ConfidenceLimits) mg/L (1)Temp. Correction-- (mg 0 2/L-min.) 1 + RC2) (2) t Inhibition@ RS x 100 Where: -1 + RC2) 2 R - Oxygen uptake rate (mg 02/L-min.) T,obs - Sample temp..,*C & a 1.047, x - T,obs - 2eC C - Control -IRRCC1 - RC (3) Difference x loo. RC 1 + RC2 2 The two control resdirationrates should be within 1S@ of each other At--E&ML-et--C,atctAlable,@oy-nexi deple-4-e-teoio Y-e6pidt@)'%16l-e-smt@AaK orie-rr-@%vAute. FQfm'.-UM-6 D.-PWO Sample Description ldv-lch 'twau3i.-C, @:at zli otoo-o Environmental Lab - Work Sheet OECD ACRIVATED SLUDGE RESPIRATION INHIBITION TEST #209 6Lab Request No. H1!A4(-) Page of Date: Analys Stock and Test Solutions Preparation STOCK SOLUTION: 5w 5-,D,%Qbln p@ievv%l(D&)/L, E.Aril IV"[.= n"-=;Ynq -Aeicie.di-.oK H.%. ic.-+3vNi iW-ID; Test Conc. ml s-to-csloln mg/L per 500 ml ml MD water per SOO ml 4 ml syn. sewage feed per 500 ml + Ila ml microbi-a-I inoculum per SOO ml Total volume ml w -F-,kG@v4@L-s wiib L17\ q 1 4 t.-S. ENVIRONMENTAL LABORATORY LR 414,Rt) 0 OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TE3T #209 RAW DATA Test Substance: Source of Activated Sludge Mixed Liquor: Date Received: V!@@w Fc.in Analyst zzm-r a @Ala5Lr,-T?rab22Cili3@lakt+Date Used: 30 MINUTE EXPOSURE iest bubstance DISSOLVED OXYGEN CONCE r4LTRATIONS malt TIME-MINUT S Concentration(mg/L) 05.5: 1.0 -1.5 1024-2.0, 2.5 13.0 3.5 4.0--1-4.515.0 CONTROL I tL44, T[t 5..5 rIc Ecr' a-ID 4.2- @S-S -6.4 jE CONTROL Il 3 - HOUR EXPOSURE - DISSOLVED OXYGEN CONCENTRAIIONS mq/-L- Test 5ubstance Concentration(mg/L) 0.5 1.0 1.5 TIME-MINUTE. TEP, 12.0 12.5 13.0 *13.5 4.0 4-.55 15.o -.5.5 Oc CONTROL 1 10.5 0 1*5 1-1."l Ill.-I1n. L4.q L4.Ll 112.!R io-ta 14 -9. g %".ONTROL II - 1!I@L4,-q-fI@4,941@3i2.sIL.117.t4 pH of Acti-vated Sludge Mixed Liquor: pH of highest concentrationtested: Test Temperature:IS-190C, Initiallpll Initial(p.73 FinaI to.ci Fina1 11.0 EWIRONME-NTAL LABORATORY Test Substance ACRIVATED SUJDGE RESPIRATION IMiIBITIO,?4TEST #209 CALCULATION OF RESULTS ic@lLir-mn@@vir, v vIre K!%4 cp N 30 - MI.MM EXposupE- Test Substance Concentration(mg/L) MNMOT. T Oxygen Uptake Rate R r)-iioL4 R Corrected(') to 2e C IiI LR 1414-R6 Date Analyst :st % Inhibitionin -RespirationRate(2) OONTROL II 2.q--I. Percent Differencein Controls(3) ECSO (951 Confidence Limits) mg/L Test Substance Concentration(mg/L) COMMOL I in Oxygen Uptake Rate R qw t)t) R Corrected(' to 2d'c 1.2-":@()L L4 Inhil)itioin Respiration Rate(2) CORML II Percent Differencein Controls(3) ECSO (9SI Confidence Limits) mg/L (1) Temp. Correction= obs e (mg 02/L-min.) RC -+ RC2) 2 RS (2)1 Inhibitionw RC1 + RC2) x 100 Where: 2 RT .uobO3xyngSeanmupplteatkeemrpa.t,e*C(mg 02/L-min.) & - 1.047, x - T,obs - 2eC C - Control (3) IRC Rc Difference- RC1 + RC2 2@2 x loo. The two control respiration rates should be within 1SI of each other 4C) So +1 +1 +1 0 'r'o- oc cr p LO o 0 OP I 1 x 9 &-tr *.26 3co ICY qcl E cr 4z C cc cc @.2 tr to TO c CIC e Lrl Ir VI W, CV) cbl E E E d 13 2E .0 2CL 11 c@ -t \r @2 cc @r V V -11 V,