Document 5knMYqMGJZMDYwLYeBYG3nr1N

AR226-2791 FOR DUPONT USE ONLY Approximate Lethal Concentration of i l H H B ^ H n B f i 9 9 E 9 n i n Rats Haskell Laboratory Report No. 276-85 E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine P.0. Box 50, Elkton Raod Newark, Delaware 19714 Date Issued: Ju1y 9, 1985 Company Sanitized. Does not contain TSCA CBI r 4, Approximate Lethal Concentration of " " -- lin Pats SUMMARY Groups of 6 male Crl : C D ( S D ) B R ^ ^ ^ twe-re e--x,posed for sing-le 4-hour . . . periods to vapor concentrations o f ^ ^ | V "j n ai.r.*...U.n.d.e.r...thceoncdolnditions of t m s study, the approximate lethal concentraron was 160 mg_/L. This material is considered to have very low toxicity by inhalation. 2- not contain T S C A CBB Conpan? Sanitized. Does Hork by: Clarence W. Hutt, 1 Technician & IT /Or T_ Robert T. Turner Technician .______6?/^> / s s f ------;-- C? ! ,.?5" Thomas Kegelman Technician Supervised by: Reviewed by: A . k U ____L Laura A. Kinney Chemist /S'/ 3 S' JLiSjJS Approved by: 0 N a n c y # Chromey, PJpl. Study Director Section Supervisor, Acute Investigations ?/ f /*S Acknowledgements: Bruce A. Burgess also participated in the conduct of this study. DPK:TAK:smk:HLR9.6 ' 3 ' company sanitized* Does not contain TSCA C B ` Haskell Laboratory Report No. 276-85 MR No Haskell No. 14.808 Material Tested: Sponsor: Material Submitted By: Test Facility: Study Initiated-Completed: Chemicals and Pigments Department E. I. du Pont de Nemours and Company Wilmington, Delaware Chemicals & Pigments Department Jackson Laboratory E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine P.0. Box 50, Elkton Road Newark, Delaware 19714 3/22/83 - 4/11/83 Distribution: -4 Company Sanitized. Dosa not contain TSC CB! INTRODUCTION HLR 276-85 The purpose of this study was to determine a 4-hour approximate lethal concentraion (ALC) f o r l ^ B w n rats. The ALC is defined as the lowest atmospheric concentrat>ont5sted that caused the death of one or more rats either on the day of exposure or within 14 days post exposure. Except as documented in the study records, this study was conducted according to the applicable Good Laboratory Practice Regulations. MATERIALS AND METHODS A. Animals Young adult male Crl :CD*(SD)BR rats were obtained from Charles River Breeding Laboratories, Kingston, New York. Each rat was assigned a unique 6-digit identification number which corresponded to h numbered card affixed to the cage. Rats were quarantined for one weex prior to testing, and were weighed and observed twice during the quarantine period. During the test, rats were housed 1n pairs in 8" x 14" x 8 " suspended, stainless steel, wire-mesh cages. The rat assigned the lower number in each cage was identified by a slash in the right ear. Rats* tails and cage cards were color-coded with water-insoluble markers so that individual rats could be identified after exposure. Except during exposure, Purina Certified Rodent ChovP #b002 and water were available ad libitum. -- B. Exposure Protocol Groups of 6 rats, 8-9 weeks old and weighing between 231-322 grams w ^ ^ g ^ o s e d '-hole-body for a single 4-hour period to a vapor atmosphere air; Rats were weighed prior to exposure and were observed for clinical signs of toxicity during exposure. Surviving rats were weighed and observed daily for 14 days post exposure, weekends excluded, except when deemed necessary by the rats' condition. C. Test Material Company Sanitized. Does not contain T SC A CBt Stability: The test material was assumed to be stable throughout the exposure phase of the study. 0. Atmosphere Generation Vapor atmospheres ofgpHfirere generated by pumping the liquid into a round bottom flask. The flaslT was at ambient temperature for the first ALC exposure. In the remaining four exposures, the round bottom flask was heated to approximately 62-121C to attain higher concentrations. The resulting vapors were carried by houseline air to the 17 liter glass exposure chamber. ..iai---- The atmospheric concentration oflj)H(p*as determined at approximately 30-minute intervals by drawing samples of the test atmosphere from the exposure chamber with a glass syringe. Samples were analyzed isothermally at 90C with a Hewlett-Packard 5790A gas chromatograph ei .ipped with a flame ionization detector and a 6 ` X 1/4" 0.0. glass column packed with 10 % SE 30 on Supelcoport 80/100 mesh. Concentrations were determined by comparison with standard curves, which were^reoared daily. Gas standards were prepared by evaporating l i q u i d | M V # n calibrated glass bottles. U^ Chamber temperature was measured ;;ith a mercury thermometer, and chamber oxygen was measured with a BioMarine Model 225 Oxygen Analyzer. F. Records Retention All raw data and the final report will be stored in the archives of Haskell Laboratory for Toxicology and Industrial Medicine, Newark, Delaware, or in the DuPont Hall of Records, E. I. du Pont de Nemours and Co., Wilmington, Delaware. - 6- Company Sanitized. Does not contain T SCA CB1 RESULTS A. Exposure Conditions and Associated Mortality Chamber temperatures ranged between 26-30C and chamber oxygen content was 21%; Atmospheric concentration and mortality data are presented below. Atmospheric Concentrations nd Associated Rat Mortality Concentration (mg/L) *fean S.O. 4.9 1.8 20 4.1 61 2.2 160 4.5 290 Range 0.17-5.7 9.7-23 56-63 150-160 280-300 Fractional Mortality # deaths/ # exposeo 0/6 0/6 0/6 5/6 6/6 B. Clinical Observations During exposure, no clinical signs of toxicity were observed in rats exposed to 4.9, 20, or 61 mg/L. Rats exposed to 160 mg/L exhibited labored breathing and salivation. Rats exposed to 290 mg/L exhibited pale ears, labored breathing, staggering and sporadic movement. All deaths occurred during exposure. The sole survivng rat exposed to 160 mg/L exhibited slight weight loss (3.0%) on the day after exposure, followed by a normal weight gain throughout the recovery period. During the 14 day recovery period, some surviving rats exhibited Slight weight loss at 24 hours post exposure. No other abnormalities were observed. CONCLUSIONS Under the conditions of this test, the inhalation ALC of 160 mg/L. This material is considered to have very low toxi n rats was inhalation. - 7Campif/iySanitized'.. Does not contain TSCffi CBfi