Document 5kg8mOjoGxeZqxkDY2bZnR16D
R&S 112377
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BIO-MEDICAL.RESEARCH
DOCUMENT DESCRIPTION FORM 63
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rtf* I 0000377
year as-1961- File number
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Author (s)f as Last Name FS (No Punctuation) and coden for journal as JAMA preceeded by one blank space
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20 21____________________40 4160 61 62
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Title of Report; end with space-hyphen-hyphen-space. Follow with Index Terms,
separated from each other with comma-space. Avoid other punctuation;
do not abbreviate.
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61 62 21
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Source (Journal, Vol., Number, Pages,Date)
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61 62 31 32
Brief Summary 1 2______ 10 SUMMARY:
61 62
61 62 63
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CENTRAAL INSTITUUT VOOi VOEDINGSONDERZOEK
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CENTRAL 'NITTTUTE FOR NUTRITION AND FOOD RESEARCH INSTrruT CENTRAL DE LA NUTRtTION ET D L'ALIMENTATION ZENTRAUNSTtTUT FOR ERnAhRUnCSFORSCHUNG
rime sequence inhalation toxicltv st.iiv .-.ith ylnvl chloride in rata dhort preliminary summary
(Incorim Information III)
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1. Conduct of the study
Cee interim information of 15th .December 1976-
2. Results available at the end of the test period of 52 seeks
2.1. 3odjr_weights
VCH m the
Body e ighta (g) at the end of week
atmosphere (ppm)
0
.- 1
12
28
0 5000
0 5000
37 '37
77 77
129 117***
103 96"*
males 386 349*"
females
215 204***
472 414"*
251 239*
52
523 44i***
291 257**
2.2. Mortality
7CM in the atmosphere
(ppm)
0 5000
0 5000
Number of deaths at week
23 32
44
males 00 00
females 00 01
0 11
0 7
52
1 23
0 22
Initial numoer of rats: 62 males and 62 females/group. After A 1J, 26 and 92 weeks IC/sec zroup were killed.
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R&S 112378
R&S 112379
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CENTRAAL INSTITUUT VOOR VO EDI N G SO N 0 E RZ O E K
since after the last interim kill at week 52 all test animals ere dead, the remaining control-s were killed and autop3ied in week
55-
2.3. Slightly decreased haemoglobin content and packed cell volume
in both sexes at week 52.
2.4. 31ood biochemistry
31ood urea nitrogen was slightly increased in females at week 26 and in both sexes at week 52. Albu-.in content of the blood serum was
* clearly decreased in males and females at week 52 only.
2.5. Serum electrolytes
rhe potassium content in blood serum was slightly increased in fetales after 4, 13 and 26 weeks. (Results obtained at week 52 not yet available).
#
2.6. Urine analyses
Volume and SOT-activity were increased, specific gravity was decreased in both sexes at week 52 only.
2.7. Liv_HSli2 33? retention was slightly decreased in both sexes after 13
and 26 weeks, but not after 4 and 52 weeks.
2.8. 25S2_I2iS!l5
Relative weights of liver and kidneys were slightly increased in both sexes at 13, 26 and 52 weeks, but not at week 4. Relative spleen weights were increased in females after 26 weeks and in both sexes after 52 weeks.
2.9. Pathology Foci of "clear" hepatocyies and small hepatocyte3 with basophilic
cytoplasm were seen in the liver after 26 and 52 weeks. Some of the most striking changes 3een in the liver after 52 week3 were: pronounced variation in the morphology of hepatooytes, extensive focal necrosis, focal proliferation of sinusoidal cells (iCunffer cells, endothelial ceils, reserve-celLsV), Large blood filled spaces often surrounded 07 reactive changes, "neo-Lvetio nodules', tumours v-rying from heoeto-
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CENTRAAL INST1TOUT VOOR VOEDINCSONOERZOEK
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cellular carcino?,as to rlupffer cei. sarcomas (an'iosarnomas?) and very anaplastic tumours.
Tumours found outside the liver in test animals that died, ere killed in extremis or at week 52 included among others ad enomas and anaplastic lung tumours, veil-differentiated, highly keratinized squamous cell carcinomas of the external ear duct (Glands of Zymoal7) and highly malignant brain tumours.
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