Document 5kbNDvrnm5eJvBJ09noY7V7E5
AR R26 -- 0754
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f9l5u,oroorocatsanaescuoimfponoanteen,twhoficFhC-m2a0y3CaEls.o b(1eOctanesulfonic acid) (CAS # 2795-39-3). R2e0m3aCrEks(2:/21/8T9)h.e 3CMurrpernotduicntfioornmaltotionnuimnbdeicrawteassit5i1s9a. mTihxteutreesotfs1a.mp8l4e% is FCfPoFaOmSer,,330.%1%diseotdhiyluemneocgtlyylcsoullfbauttey,l 2et.h1e%r,p6o0ly.o2x3y%etwhaytleern,e2m.o5n6o%ocStuylltpohneeny! ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. cThhaerafocltleorwiiznegd csounmcmeantrryataipopnlisoesftiompaumriitxiteusr.eDwaittha imnacyomnpoltetaeclcyurately reflect the toxicityofthe fluorochemical componentofthe test sample.
METHOD
Method: OECD 209 GLP: No
Year Completed: 1990
Analytical monitoring: Dissolved oxygen concentrations, pH and
tSetamtpiesrtaitcuarlem.ethods: Results were determined by calculationofthe % Tinehsibtitoirognainn irsesmpisroautriocne:rateA.ctivated sludge mixed liquor collected from the TMeetsrtocWonadsitteioTnr:eatment Plant, St. Paul, MN
SDiylnutthieotniwcasteerw:agMei:llippeorreOMEiClDi-mQeTMtwhaotder Reference andtestsolution preparation: A stock solution of the 5re0f0ermegncien s1u0bsmtLan1cNe,Na3,O5H-,dicdihlluotreodphteono3l0,mwLawsitphreMpialripeodrbeyMdiilsis-oQlvTMing waantderd,iuttheedn tboro1uLghwittthotMhiellp-oQiTMntwoafteirnc.ipTihenetpprHoecfipitthaetiroenfweirtehnc1eNsoHlzuStiOo,n wveasssemlesaasnudrewdastoabpepa7r.e4n.tlTyesmtisscuibbslteawnicthe wwaatsera.dded directly to test TNeusmtbveerssoeflsc:oncNeonttgriavteinons: three plus 1 reference substance and 2
Tbleamnpkercoantturroels: 20-22 C Total suspended solids and
pH on
dayoftesting:
5.033 g/L and
---
winaitsial2.p0H206.g6ILa,npdHfi6na.l6paHnd6.f9i.nalInTitSiaSl TofS1S0o0f01m0g0/0Lmcgo/nLcecnotnrcaetnitornawtiaosn
22009, pH 68.
001122
Element Basis: Respiration inhibition as determinedby oxygen `consumption.
Remarks: Values corrected to 20 C for calculations.
RESULTS
00000000 000
Nominal concentrations: 2 Blank controls, reference substance, 320, 560
and 1000 mg/L Element values:
30-minute ECs=o >1000 mg/L
3-hour ECso = >1000 mg/L.
Element values based on nominal concentrations.
Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not
the fluorochemical proportion alone.
CONCLUSIONS
_
The test substance 3-hour ECso for activated sludge respiration inhibition was determined to be >1000 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St.
Paul, Minnesota, 55133
DATA QUALITY
r--------
Reliability: Kiimisch ranking 2. However, sample purity was not
Testing properly
meets all criteria for quality testing. characterized and the study lacks
analytical confirmationofthe amountoffluorochemical proportion in the
solution.
REFERENCES
_
TPhauils,sMtuNd,yLwaabsRceoqnudeuscttendumbbyetrheH134M80C-o1m,pa19n8y0,.Environmental Laboratory, St
OTHER
Last changed: 6/26/00
001123
N
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE MIXED LIQUOR
Tl idelicmaile wi Date.
Analys: S
Type Test ActivatedSludge Respioation lnnibitionTest OECTEZ0
Source MeynWaste TreatmentPak,StPadMa
Date Obtatned__%/1a/90
Date Used__%/14/90
Mixed Liquor Suspended Solids (MLSS) ,0x% g/L Adjustment
BIL
PH_igitial late, allnG
~
Comente_______.
Test Substance
Sample Description,
some pH AAS highest er
rasvoent
Comments MLSS of 1000mgfl test Sein luitial 202.g/ls, Tioal(x\228
Reference Substance
Sample Description 3S = Ili Sample PH Wianihteisalt to Sonas l lkak: 10A) dustaent
Coments, Sock solu, pI MM
eeeeerrer--
Exposure Conditions
Test Duration XMoues
Test Temperature 20-22
c
-
Comments_
----
--_
---
--_----------------------------ooT24
ample Description
Environmental Lab -- Work Sheet
a
RESPI(ROAETCIDOANCTIINVHAITBEIDTISOLNUTDEGSET #209
"
oad .
Lab RequestNo. HIRO]
"0 --1-- of S--
oae: _x/19/a0
prayer "ZF3S
efeset Cronc. ng addedeemle m wee arteemrereeemely m syn. sewage e ml mis crobial i eesTe ots alrvot le
220
len + PYRE
o
+ 200
ALO
se + spd + . + 200
1000*
500 + 28
+
AContoll 6 + zag +
2
+
200
. + 200
= S00
= sm
= S00
= soo
Very foamy.Large amount.oof foam homed,
i Se riom------me-- ee -- seep---- r-- e-- i
001125
So
ENVIRONMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 RAW DATA
Rese torE
Test Substance: LCRORCE, Loot 59 jasfer)
Source of Activated Sludge MixedLiquor: Mets Waste Torabseset Plant, St Bue bli
Date Recefved: S//g/90
Date Used: 3/9/90
Mnalyst: ZZclpele"Xabiidecu
30 - MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS mo
CONTROL
2.
9
Q
2
5
9125
noo
CONTROL 11
3 - HOUR EXPOSURE
EST Substance
CONTROL I
EEEEEERRREE |soluslu|elo17TBg|d|
Cerrrrrrr
nalozlwolala som zl z aleelie bo
92
DISSOLVED OXYGEWHE-HCIONNUCTEEN:TRATIONS ng
TH
2 Wh
2.2|2.42.0 |2
EEEEERREEREA
comma 1
lsalssloiluslyelaslzaladzslziliel
PH of Activated Sludge Mixed Liquor:
TPeHstofTehmipgehreastturceo:nc2e0nt-r2a2tioCn. tested:
Initial b.bo Initial L.lo
Final b.9 Final 4.8
001126
ENVIRONMENTAL LABORATORY
LR A480 -L
.
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
Date 3
:
CALCULATION OF RESULTS
a.5
Test Substance L-203CE. Lot NP(22189)0
30. MINUTE EXPOSURE
ConcTeensttraStuibosntan(cneg/L) Oxygen UpRtake Rate RrtoCo2r0pCected!) + RIenshpiibriattioinoniRante,(?)
CONTRO)
|_og|4 osa u 7|
0
|_ioses | losgs | -- ~ 3s
20
|Lzde | ums | --
000 [en
1den | -- Ay
1
CONTRO)
|ose |ox nano|
Percent Difference in Controls) ECgy (95% Confidence Limits) mg/l
____% 2,%%
GR ROS
ConTceesnttraStuibosntan(cneg/L)
cor)
Q 20 00a
oeenen UpUptake Rate
RtoC2o0rrCecteTMd) %
| oso
| anwis |
| oo1za |oqva=|
|071 |o0 v109 a |
|om| a ondr er |
-
RIenshpiibriattiioonniRnate(?) 0 o
CONTRO
[c | fw | oo 7esee|
Percent Difference in Controls)___2.5%
:
EC. (95% Confidence Limits) mg/L mmo
ooo
(1) Tew.Correctionz--[5 L (ng 0,/L-min.)
(2) Inhibitions:
+ RC 7
Se X 100
RC, + RC.
The=reO:xygen uptake rate (ng2 O,/L-nin.)
T,obs = Sample temp. ,*C
6 C = C=1o,n0t4r7,ol x=Tob - 20C
(3) % Difference H ( +d) 100 The two control respir:ation rates should be within 15% of each other
001127
SM-- ErL anD bSaolriadts[TS]A[o158O][tTaDsSh]at
sot onsRDPSET]Se 500
i[n=l
i
5
=[mo]om[en[| on[oem| on ----
Yor LRHe INEO-1r ,3b,b as 5,5-Dcfet
Sm] lc Times [ues[oww|on |
mot
Ts es | wuss| acess| souo [oXesosseizegh
t | ides e|russ| sets |soz0 PD
| Ten|wer|coon|2020 [X00 20mg
|TT s iesa [was|oom2220Prowstiem]
--
rrrrT
TrTTT
TT 1I
a5m5pl8e Desclroipttiionon 31%
rir re
hiol t aDo,s0,6W0i-s0
Lab Request No.H1HBQ
Environmental Lab -- Work Sheet
RESPIOREACTIDOANCTIINVHAITBIETDIOSNLUTDEGSET #209
P20 --L-- of 3 Date:
Analyst:
Stock and Test Solutions Preparation
STOCKSOLUTION: 500mg A S-2cp)
Each Im! =0.5mg
Cestoncm.l stock soln mM) water ml syn. sewage
ng/L
2 500 ml
EL 500 ml feedper 500 ml
10
10 + 2 ow
.
3 Cubed TO + oon +
e
Wl microbial
Total volume
inoculum REL, 500 ml
ml
+ 200 + 200
= sen = 500
'
001129
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LR45
ENVIRONMENTAL LABORATORY
Le ZFS
OECD ACTIVATED SLUDGE RESPIRATION INIIDITION TEST #200
RAK DATA
+
Test Substance:3,5-Di(RcefehrenlceSoubr stao neeg ) iennl
Source of Activated Sludge MixedLiquor: MemWaste TreatmentPlank,SkPaulMu.
Date Received:__%/19/d0
Date Used: _=/19d0
malysteZacpelle.Fnbidesu
30 - MINUTE EXPOSURE TST Subs ance
DISSOLVED OXYGEN CONCENTRATIONS. 0g HE-HTNG
: TH
CONTROL Tp
8
9
o
a
oul
BEEEERERE=EE FES
rirrrrrrrtrtd
NI rrrrrrrr
1 PT FPS YF PYPe
3 = HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS mo
5T Substance
WE-HINUTES
TE
como x Concentration
(mg/L)laa [ns[ma [use
[uz[xa[an[aafoslon
c
[ue fia
ee Ctrrrrrrrror
rrrrrrrrrrrr
-- [ss [solu
PH of Activated Sludge Mixed Liquor: PTHestofTehmipgehreastturceo:nc2e1n-tr2a2t.ionC.tested:
.
lu [ae salrelozdiaTiaTuo 2)
Initial bolo
Initial 6,1
Final 04
Finalb.8
001130
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
rest. Substance 5, S=DickiloeaplienCiAoLlCULTATeIOpNlOFiRcESeULTSSubstaure)
EE 30 - MINUTE EXPOSURE Test Substance CONTROL 10
gen Uptake Rate R Corpected0
|_o8| zov= 8kl0|
| osisz. [| onan |
pate =
Ae
S
+ Lie InhibiT tion in
8
CONTROL, ITY
|ogm | oa mast a |
Percent Difference in CantrolsTM __/ 47
SQ EXPOS BCgo (95% Confidence Limits) mg/l
Test Substance
en Uptake Rate
R Corrected (1)
cCONToROL ri a) |om |owe as, | a
|_ozsNz, |oid |
C7
% EE Inhibivitedi, on i% n
2
CONTROL I
|
1
|oca| ooss ziezs , |
Percent Difference in Controls)___.5/%
:
Egg (95% Confidence Limits) mg/l
() Temp.Correctionz --L2 (ng 0,/L-nin.)
&
RC, (2) % Inhibition:
+ RC.
2.
ew) Se
x 100
(3) 3Diferences--l----dx 00
RC, + =)
2
The two controlrespiration rates
should be within 15% of each other
WRhe=rOex:ygen uptae ke raS te(ng O,/L-nin.)
T,obs = Sampletemp.,'C . 2 6C'==1C,o0n4t7r,ol x = T,obs - 20C
BEST COPY AVAILABLE
001131